An agricultural fungicidal composition containing WML-01 and fludioxonil, preparation and application thereof

Abstract

The application discloses a bactericidal composition containing WML-01 and fludioxonil, and active ingredients of the bactericidal composition include WML-01 and fludioxonil, and the mass ratio of the WML-01 and the fludioxonil is 10:1-1:10. The bactericidal composition can be used for preventing and treating rice false smut, wheat basal stem rot, wheat scab, soybean root rot, rice blast, pepper anthracnose and rape sclerotinia rot, can effectively solve the problems of less bactericides for preventing and treating the diseases, unstable prevention and treatment effect, high prevention and treatment cost and drug resistance in current production, and has a wide market application prospect.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, and in particular to an agricultural fungicide composition containing WML-01 and fludioxonil, its formulation and application. Background Technology

[0002] Rice bakanae disease, also known as excessive growth disease, is a significant disease affecting rice production. The main pathogen is *Fusarium oxysporum* (Fusarium oxysporum). Fusarium fujikuroi Infected seeds and diseased plant debris in the field are the main primary sources of infection for rice bakanae disease. Infected grains often fail to germinate or emerge after sowing. Typically, infected seedlings are thinner and taller than healthy seedlings, with slender leaf sheaths, pale yellow leaves, and poor root development; some seedlings die before transplanting. After transplanting, the mycelium can spread throughout the entire plant under suitable conditions, stimulating excessive vegetative growth. During flowering, the pathogen spreads to the flowers, invading the glumes and endosperm, causing shriveled or deformed grains. If the pathogen invades late, although the grains may not show symptoms, the mycelium has already penetrated internally, making the seeds infected and affecting the planting of subsequent rice crops, causing significant damage. Chemical control remains the primary method for controlling rice bakanae disease. Currently, fungicides registered for the control of rice bakanae disease on the market include carbendazim, prochloraz, difenoconazole, and fludioxonil. However, the long-term, large-scale, and frequent use of chemical agents has led to pathogens gradually developing resistance to different fungicides, resulting in a significant reduction in the effectiveness of the agents. Increasing the amount of pesticides used will lead to problems such as pesticide residues, seed safety, and environmental pollution.

[0003] Wheat stem base rot, commonly known as "crown rot" or "dryland foot disease," is caused by Fusarium graminearum (…). Fusarium pseudograminearum Fusarium graminearum ( ), Fusarium graminearum This is a global wheat stem base rot caused by various Fusarium fungi, including [list of fungi]. Early symptoms of wheat stem base rot include browning and rotting of young roots or coleoptiles, leading to seedling death in severe cases. The disease then spreads to the first and second stem nodes, causing the affected areas to turn dark brown or rot. The leaf sheaths at the stem base wither, and the stem turns brown and necrotic. Later, the browning at the stem base hinders the transport of water from the roots to the ear, resulting in a completely white ear. After the ear forms a "white ear," the wheat grains cannot continue to develop, significantly impacting the grain filling rate and yield. Currently, only eight pesticide products are registered for the control of this disease, including difenoconazole, tebuconazole, fludioxonil, prothioconazole, triflupyridine, and thiamethoxam. There is an urgent need for new pesticides to control this disease.

[0004] Fusarium head blight, commonly known as "wheat rot," is a global wheat disease caused by various Fusarium fungi, including Fusarium graminearum. F. graminearumFusarium head blight (FHB) is a dominant pathogen in many wheat-producing areas. It causes seedling blight, stem rot, and ear rot; in epidemic years, the disease incidence rate often exceeds 50%, resulting not only in a significant reduction in yield and deterioration in quality, but also in the presence of toxins in the diseased grains, which can cause poisoning in humans and animals. Fungicides used to control FHB in production mainly include benzimidazole fungicides, triazole fungicides, methoxyacrylate fungicides, and methoxyacrylate fungicides. The long-term and extensive use of chemical fungicides has led to increasingly prominent resistance issues to the FHB fungus.

[0005] Root rot is a common disease in plant production. The pathogen primarily damages the plant roots, causing poor plant growth, seed rot, root decay, stunting, yellowing flowers, and in severe cases, complete plant death, leading to significant yield losses. Soybean root rot is a major root disease in soybean production, with pathogens including Fusarium, Rhizoctonia solani, and Pythium. In the seedling stage, root rot affects seedling growth and can even cause seedling death, reducing the number of seedlings remaining in the field. In mature plants, root damage affects the growth and number of root nodules, resulting in poor above-ground growth, stunting, and reduced pod number and grain weight, thus lowering yield. Currently, fungicides registered for the control of soybean root rot include difenoconazole, prochloraz, fludioxonil, carbendazim, and thiram.

[0006] Rice blast, also known as rice fever, fire blast, or knocking blast, is one of the important diseases of rice. The pathogen is *Pyrrosia oryzae* (rice blast). Pyricularia oryzae Rice blast (Cav.) mainly includes seedling blast, leaf blast, node blast, neck blast, and grain blast, with neck blast and node blast being the most severe, causing yield losses of 10%-30% in mild cases and 40%-50% or even total crop failure in severe cases. Fungicides used in production to control rice blast mainly include tricyclazole, carbendazim, isoprothiolane, prochloraz, pyraclostrobin, and jinggangmycin. The long-term and extensive use of chemical fungicides has led to increasingly prominent issues of herbicide resistance in rice blast.

[0007] Anthracnose is a common plant disease that primarily affects leaves, fruits, and branches, causing sunken lesions. These lesions are often covered with numerous small black dots (conidiophores), severely impacting the yield and quality of plant products. The pathogens causing anthracnose are mainly fungi of the genus *Anthracnose* (*Hylocereus*). Colletotrichum spp.) fungi, including collodion fungus ( Colletotrichum gloeosporioides Anthracnose is a representative species that can cause anthracnose in plants such as citrus, mango, pomegranate, grape, strawberry, pepper, tomato, ginger, onion, rubber, corn, sugarcane, and various flowers. Currently, the main pesticides for controlling anthracnose include fludioxonil, methoxyacrylates, and benzimidazoles. While there are many pesticide products available, the variety is relatively concentrated; products containing difenoconazole and pyraclostrobin account for over 25% of the total. This long-term, concentrated use of chemical pesticides has led to increasingly prominent problems with anthracnose resistance.

[0008] Sclerotinia sclerotiorum is the most serious disease of oilseed rape, and the pathogen is Sclerotinia sclerotiorum. Sclerotinia sclerotiorum The disease can infect the stems, leaves, flowers and silique of oilseed rape, among which the stem is the most seriously affected. After the disease occurs, water-stained, light brown lesions appear on the stems of oilseed rape, and the central part of the lesions turns white in the later stage, with brown edges, and the boundary between the diseased and healthy parts is obvious. White fluffy mycelium grows on the lesions in humid conditions, eventually leading to stem rot, breakage, and the death of the whole plant. At present, there are very few pesticide varieties for the prevention and control of oilseed rape sclerotinia, most of which are concentrated in carbendazim, procymidone and fludioxonil. The resistance of Sclerotinia sclerotiorum to carbendazim has already occurred seriously. There is an urgent need for new pesticide varieties for the prevention and control of the disease to replace carbendazim and control the occurrence of resistance.

[0009] Fludioxonil belongs to the pyrrole fungicide, and its mechanism of action is to interfere with and destroy the biological oxidation and biosynthesis process of the pathogen, and to destroy the synthesis of nucleic acids and proteins to cause the death of the pathogen. It has good control effect on gray mold, sclerotinia, damping-off, root rot, wilt, and wilt of various crops. Fludioxonil has no systemic conductive activity, but has significant protective activity and high safety, and is widely used for seed treatment of crops. At present, there are 305 pesticide products containing fludioxonil registered in China, of which 111 are used for the prevention and control of plant root rot, 72 are used for the prevention and control of rice sheath blight, and 2 are used for the prevention and control of wheat scab. The development trend in the future is to combine fludioxonil with highly systemic active agents to prevent and control plant diseases.

[0010] At present, there are few pesticide varieties for the prevention and control of rice sheath blight, wheat foot rot, wheat scab, plant root rot, rice blast, plant anthracnose and oilseed rape sclerotinia. With the long-term use of single-mechanism selective agents, the pathogen has developed resistance to varying degrees. It is of great significance to develop new fungicides with novel mechanisms of action, and to reasonably combine them with fludioxonil, which has broad-spectrum, high efficiency and significant protection, to reduce the amount of pesticide and improve the efficacy, and to delay the occurrence of resistance, control the occurrence of disease and prolong the life cycle of the pesticide. SUMMARY

[0011] The purpose of the present application is to provide an agricultural fungicidal composition containing WML-01 and fludioxonil, as well as its preparation and application, to solve the technical problem that there are few pesticide varieties for the prevention and control of rice sheath blight, wheat foot rot, wheat scab, plant root rot, rice blast, plant anthracnose and oilseed rape sclerotinia, and with the long-term use of single-mechanism selective agents, the pathogen has developed resistance to varying degrees.

[0012] In order to achieve the object of the present application, the first aspect of the present application provides an agricultural fungicidal composition, wherein the active ingredient of the agricultural fungicidal composition comprises WML-01 and fludioxonil, the mass ratio of the WML-01 and the fludioxonil is 10:1-1:10, the WML-01 is a butenolide compound containing thiazolidine structure, and the structural formula is shown in formula I:

[0013] .

[0014] In an alternative embodiment, the mass ratio of the WML-01 and the fludioxonil is 10:1-1:1.

[0015] In an alternative embodiment, the mass ratio of the WML-01 and the fludioxonil is 3:1-1:10.

[0016] The second aspect of the present application provides a fungicidal preparation, wherein the fungicidal preparation comprises the agricultural fungicidal composition.

[0017] In an alternative embodiment, the dosage form of the fungicidal preparation is seed coating agent, suspension agent, water emulsion, microemulsion, emulsifiable concentrate, microcapsule suspension preparation, nano preparation, granule, wettable powder or water dispersible granule.

[0018] In an alternative embodiment, the components of the fungicidal preparation further comprise adjuvant or carrier for assisting the agricultural fungicidal composition, the adjuvant is xanthan gum, wetting agent, dispersing agent, defoaming agent, antifreeze or warning color.

[0019] In an alternative embodiment, the wetting agent is nonylphenol polyoxyethylene ether NP-10, alkylphenol polyoxyethylene ether OP-10 or alkyl naphthalene sulfonate EFW; the dispersing agent is sodium salt D-425 of alkyl naphthalene sulfonate condensate or sulfonate D-450 of alkyl naphthalene sulfonate condensate; the defoaming agent is n-octanol or silicone SAG1522; the antifreeze is ethylene glycol; and the warning color is basic roseine, gold red or fast peach red.

[0020] The third aspect of the present application provides application of the fungicidal composition or the fungicidal preparation in plant disease prevention and treatment, wherein the plant disease is one or more of rice seedling blight ( Fusarium fujikuroi ), wheat basal stem rot ( Fusarium pseudograminearum ), wheat scab ( Fusarium graminearum ), soybean root rot ( Fusarium spp.), rice blast ( Pyricularia oryzae ), pepper anthracnose ( Colletotrichum spp.) or rape sclerotinia ( Sclerotinia sclerotiorum ).

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The agricultural fungicide composition provided by this invention, with WML-01 and fludioxonil as active ingredients, has a synergistic effect on the control of rice seedling blight, wheat stem base rot, wheat scab, plant root rot, rice blast, plant anthracnose, and rapeseed sclerotinia rot. It can effectively improve the control effect of diseases, reduce the amount of pesticides used, delay the occurrence of pesticide resistance, and increase crop yield. It is safe, effective, and economical, and has broad market application prospects. Detailed Implementation

[0023] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0024] Agricultural fungicidal compositions and their formulations can be applied in various ways, and this invention does not impose any particular limitation. One or a combination of various application methods can be used, such as seed coating, seed soaking, spraying, root drenching, broadcasting, hole application, and furrow application. Depending on crop cultivation, formulation type, and target pests, seed coating, seed dressing, and seed soaking are preferred methods for this fungicidal agricultural composition.

[0025] Example 1: In vitro antibacterial activity of WML-01 and fludioxonil against *Bacillus bakanae*, *Bacillus thuringiensis*, *Fusarium graminearum*, *Fusarium graminearum*, *Bacillus oryzae*, *Sclerotinia sclerotiorum*, *Sclerotinia sclerotiorum*, and *Anthracnose* of peppers.

[0026] 1. Test materials

[0027] 1.1 Test strains

[0028] Rice seedling blight pathogen ( F. fujikuroi (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0029] Soybean root rot pathogen ( F. oxysporum (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0030] Wheat stem rot fungus ( F. pseudograminearum (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0031] wheat scab (Fusarium head blight) F. graminearum (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0032] Rice blast fungus ( P. oryzae (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0033] Sclerotinia sclerotiorum (Lib.) de Bary (provided by the College of Plant Protection, China Agricultural University), identified by routine methods. S. sclerotiorum

[0034] Colletotrichum capsici (Atkinson) E. Tulasne (provided by the College of Plant Protection, China Agricultural University), identified by routine methods. C. gloeosporioides

[0035] 1.2 Test agents

[0036] WML-01 (99% technical material), provided by the School of Science, China Agricultural University. The compound and its preparation method are disclosed in Patent Publication No. CN 118724887 A, and the compound is numbered as 3-1.

[0037] The structural formula is shown in Formula I:

[0038]

[0039] Fludioxonil (98% technical material), product of Shangyu Yingtai Fine Chemical Co., Ltd.

[0040] The above-mentioned pesticide technical materials were respectively prepared into mother liquor with a concentration of 10 mg / L by using dimethyl sulfoxide as the solvent, and the mother liquor was stored in a 4℃ refrigerator for standby use. 4 mg / L by using dimethyl sulfoxide as the solvent, and the mother liquor was stored in a 4℃ refrigerator for standby use.

[0041] 1.3 Test medium

[0042] Potato dextrose agar (PDA): 200 g of potato, 18 g of glucose, 12 g of agar powder, and 1000 mL of distilled water.

[0043] 2 Test method

[0044] The test was carried out in accordance with NY / T 1156.2-2006 Guidelines for Pesticide Indoor Biological Test Inhibition of Pathogenic Fungus Mycelium Growth Test Plate Method. The main operation process of the plate method is as follows: 5 concentration gradients are set between 10% and 90% of the inhibition rate of the agent on the target fungus, then a puncher with a diameter of 6 mm is used to take fungus cakes on the same circumference near the edge of the activated target fungus (to ensure that the fungus age of the same repeat of the test pathogenic fungus is the same), and a inoculation needle is used to inoculate the fungus cakes to the center of the series of drug-containing medium plates under sterile conditions, with the mycelium facing down, and a plate without drug is set as a control, and placed in the dark for culture. Each treatment is repeated 3 times for determination. The colony diameters of each concentration treatment are recorded, and the mycelium growth inhibition rate is calculated. Then the inhibition rate is converted into a probability value (Y), and the concentration of the agent is converted into a logarithmic value with 10 as the base (X), the X-Y is made into a regression straight line, and the toxicity regression curve equation Y=a+bX of each agent on the target fungus, as well as the correlation coefficient r and the effective inhibition medium concentration EC 50 .​​

[0045] The joint toxicity of the complex preparation is expressed by the method of co-toxicity coefficient of Sun Yunpei:

[0046] Theoretical toxicity index of the complex preparation TTI =∑(the toxicity index of a drug × the percentage of the active ingredient of the drug in the mixture)。

[0047]

[0048] CTC ≧120 is synergistic effect; CTC ≦ 80 is antagonistic effect; 80 CTC <120 is additive effect.

[0049] 3 Test results

[0050] Table 1 In vitro antibacterial activity of WML-01 and fludioxonil complex on Pyricularia grisea

[0051]

[0052] The above results show that: WML-01 and fludioxonil in the range of 10:1~1:10 (w / w) complex, the co-toxicity coefficient of Pyricularia grisea is between 84.21~169.37, showing additive and synergistic effect. Among them, when the mass ratio of WML-01 and fludioxonil is in the range of 10:1~1:1, the co-toxicity coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 169.37.

[0053] Table 2 In vitro antibacterial activity of WML-01 and fludioxonil complex on Gaeumannomyces graminis

[0054]

[0055] The above results show that: WML-01 and fludioxonil in the range of 10:1~1:10 (w / w) complex, the co-toxicity coefficient of Pyricularia grisea is between 84.21~169.37, showing additive and synergistic effect. Among them, when the mass ratio of WML-01 and fludioxonil is in the range of 10:1~1:1, the co-toxicity coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 169.37.

[0056] Table 3 In vitro antibacterial activity of WML-01 and fludioxonil complex on Gaeumannomyces graminis

[0057]

[0058] The above results show that: WML-01 and fludioxonil are mixed in a mass ratio of 10:1~1:10 (w / w), and the synergistic coefficient of wheat scab fungus is 98.4~182.13, which shows additive and synergistic effect, and when the mass ratio of WML-01 and fludioxonil is in the range of 3:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 182.13.

[0059] Table 4 In vitro antibacterial activity of WML-01 and fludioxonil after compounding on soybean root rot fungus

[0060]

[0061] The above results show that: WML-01 and fludioxonil are mixed in a mass ratio of 10:1~1:10 (w / w), and the synergistic coefficient of wheat scab fungus is 98.4~182.13, which shows additive and synergistic effect, and when the mass ratio of WML-01 and fludioxonil is in the range of 3:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 182.13.

[0062] Table 5 In vitro antibacterial activity of WML-01 and fludioxonil after compounding on rice blast fungus

[0063]

[0064] The above results show that: WML-01 and fludioxonil are mixed in a mass ratio of 10:1~1:10 (w / w), and the synergistic coefficient of wheat scab fungus is 98.4~182.13, which shows additive and synergistic effect, and when the mass ratio of WML-01 and fludioxonil is in the range of 3:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 182.13.

[0065] Table 6 In vitro antibacterial activity of WML-01 and fludioxonil after compounding on Sclerotinia sclerotiorum

[0066]

[0067] The above results show that: WML-01 and fludioxonil are mixed in a mass ratio of 10:1~1:10 (w / w), and the synergistic coefficient of wheat scab fungus is 98.4~182.13, which shows additive and synergistic effect, and when the mass ratio of WML-01 and fludioxonil is in the range of 3:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 182.13.

[0068] Table 7 In vitro antibacterial activity of WML-01 and fludioxonil after compounding against Colletotrichum gloeosporioides of pepper

[0069]

[0070] The above results show that: WML-01 and fludioxonil are compounded at a mass ratio of 10:1~1:10 (w / w), and the co-toxicity coefficients of the two against Colletotrichum gloeosporioides of pepper are between 80.24~191.82 by the co-toxicity coefficient method, showing additive and synergistic effects. When the mass ratio of WML-01 and fludioxonil is in the range of 5:1~1:1, the co-toxicity coefficients are all greater than 120, showing synergistic effects, and the synergistic effects are significant, with the highest reaching 191.82.

[0071] Example 2 Field control effect of WML-01 and fludioxonil compounding on wheat scab

[0072] 1. Materials and methods

[0073] 1.1 Test materials

[0074] 10% WML-01·fludioxonil suspension concentrate, self-made, containing WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, and the balance water, % being mass percentage content, prepared by sand milling process; 10% WML-01 suspension concentrate, self-made, containing WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, and the balance water, % being mass percentage content, prepared by sand milling process; 30% fludioxonil suspension concentrate, product of Jiangxi Zhengbang Crop Protection Co., Ltd.

[0075] 1.2 Test site profile

[0076] The test was conducted in Fengtai County, Anhui Province, with clay loam soil, medium fertility, wheat variety Huaiyumi No. 1, and seed rate of 25 kg per mu.

[0077] 1.3 Test design

[0078] Table 8 Test scheme of seed treatment on wheat scab

[0079]

[0080] 1.4 Investigation method and calculation

[0081] Randomly sample 5 points per treatment area, 100 ears per point, and grade according to the percentage of ear area affected by disease to the whole ear area, calculate disease index and control effect.

[0082] Gibberella disease grading standards:

[0083] 0, no disease on the whole ear;

[0084] 1, the area of withered ear is less than 1 / 4 of the whole ear area;

[0085] 3, the area of withered ear is 1 / 4-1 / 2 of the whole ear area;

[0086] 5, the area of withered ear is 1 / 2-3 / 4 of the whole ear area;

[0087] 7, the area of withered ear is more than 3 / 4 of the whole ear area;

[0088] Pharmacodynamic calculation method:

[0089] Disease index (%) =∑(number of plants (ears) of each level × representative value of each level) / (total number of plants (ears) surveyed × representative value of the highest level) × 100

[0090] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100

[0091] 2 Test results

[0092] Table 9 Effect of pesticide treatment on wheat Gibberella disease

[0093]

[0094] Note: * The average value of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0095] From the contents in the above table, it can be seen that after 2 times of spraying, the high dose 200 ml treatment (effective ingredient 20 g) of 10% WML-01·fludioxonil suspension agent has a control effect of 90.49% on wheat Gibberella disease, which is obviously better than that of fludioxonil single agent treatment (effective ingredient 12 g) and WML-01 single agent treatment (effective ingredient 20 g); the low dose 100 ml treatment (effective ingredient 10 g) has a control effect of 85.41% on wheat Gibberella disease, which is equivalent to WML-01 single agent treatment and better than fludioxonil single agent treatment.

[0096] The above results show that after the compounding of WML-01 and fludioxonil, excellent control effect on wheat Gibberella disease is shown at the same dose as fludioxonil single agent, and the field use amount is reduced compared with WML-01 single agent treatment.

[0097] Example 3 Field control effect of WML-01 and fludioxonil compounding on soybean root rot

[0098] 1 Materials and methods

[0099] 1.1 Test materials

[0100] 10% WML-01 + fludioxonil SC, self-made, wherein WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance being water, % being mass percentage content, prepared by sand milling process; 10% WML-01 SC, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance being water, % being mass percentage content, prepared by sand milling process; 25 g / L fludioxonil seed treatment SC, produced by Beinong Haile (Zhuozhou) Seed Coating Ltd.

[0101] 1.2 Test site profile

[0102] The test site was located in the modern agricultural demonstration park of Beian City. The soil type was black soil, with an organic matter content of 5.6% and a pH value of 6.6. Sowing was carried out on May 21, 2024, using a 65 cm ridge three-cultivation mode, with 320,000 seedlings per hectare. 48% special soybean fertilizer was used, with a fertilizer application rate of 320 kg per hectare (N:P:K = 15:23:10). The seedlings emerged on June 1. The soil moisture content was good. The previous crop was soybean. Natural precipitation was relied on.

[0103] 1.3 Test design

[0104] Table 10 Test scheme of seed treatment on plant root rot

[0105]

[0106] 1.4 Investigation method and calculation

[0107] Soybean root rot investigation

[0108] Investigation was carried out once every 30 d and 60 d after the soybean seedlings emerged. Not less than 30 soybean plants were randomly dug from each plot for investigation, and the total number of plants and the number of plants at each level were recorded. The incidence of root rot was investigated, and the control effect was calculated.

[0109] Disease classification: (root lesion area)

[0110] 0 level: main root, fibrous root is healthy, no lesion, nodule is more;

[0111] 1 level: sporadic lesions on the main root, but not in pieces, a few lesions on the fibrous root;

[0112] 3 level: main root lesion is scattered, but less than 1 / 4 of the root area, fibrous root is slightly diseased;

[0113] 5: The area of main root disease spot is between 1 / 4 and 1 / 2 of the root area, and the fibrous root disease is more but not in pieces;

[0114] 7: The area of main root disease spot is between 1 / 2 and 1 / 4 of the root area, and the fibrous root disease is in pieces, and part of the fibrous root falls off;

[0115] 9: The whole root is surrounded by disease spot, and the root is rotten, and the fibrous root is almost none.

[0116] The efficacy calculation method is as follows:

[0117] Disease index (%) =∑(number of each level of diseased plants (ears) × each level of representative value) / (total number of plants (ears) surveyed × highest level of representative value) × 100

[0118] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100

[0119] 2 Test results

[0120] Table 11 Effect of the fungicide treatment on soybean root rot

[0121]

[0122] Note: * The average value of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0123] From the above table, it can be seen that 30 days after emergence, the 10% WML-01·fludioxonil suspension agent high dose 200 ml seed dressing treatment (effective ingredient 20 g) and 100 ml seed dressing treatment (effective ingredient 10 g) have an effect of 80.61% and 77.57% on soybean root rot, which is significantly better than the WML-01 single agent (effective ingredient 20 g) and the fludioxonil single agent treatment (effective ingredient 10 g).

[0124] 60 days after emergence, the 10% WML-01·fludioxonil suspension agent 100 ml and 200 ml seed dressing treatment have excellent effects on soybean root rot, which are 86.01% and 91.02% respectively, which are significantly better than the WML-01 single agent and the fludioxonil single agent treatment.

[0125] The above results show that after the WML-01 and fludioxonil are compounded, at the same dose as the fludioxonil single agent, excellent effects on soybean root rot are shown, and compared with the WML-01 single agent treatment, the field drug dosage is reduced while the effect is improved.

[0126] Example 4 Field effect of WML-01 and fludioxonil compound on wheat foot rot

[0127] 1 Materials and methods

[0128] 1.1 Test materials

[0129] 10% WML-01 + fludioxonil SC, self-made, wherein WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is mass percentage content, prepared by sand mill process; 10% WML-01 SC, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is mass percentage content, prepared by sand mill process; 25 g / L fludioxonil seed treatment SC, produced by Beinong Haile (Zhuozhou) Seed Coating Co., Ltd.

[0130] 1.2 Test site profile

[0131] The test was set in Feixiang District, Handan City, Hebei Province, with clay loam soil, medium fertility, wheat variety Woyu No. 3, and seed rate of 20 kg per mu.

[0132] 1.3 Test design

[0133] Table 12 Test scheme of seed treatment on wheat stem base rot

[0134]

[0135] 1.4 Investigation method and calculation

[0136] Stem base rot investigation

[0137] a) Investigation of control effect of stem base rot at the filling stage

[0138] Randomly sample 5 points per treatment area, 100 plants per point, investigate the number of diseased plants and disease index, and calculate disease index and control effect.

[0139] Stem base rot grading standards:

[0140] 0 level, plant not diseased;

[0141] 1 level, stem brown in the ground;

[0142] 3 level, first internode brown;

[0143] 5 level, second internode brown;

[0144] 7 level, third internode appears brown symptoms, but no white head;

[0145] 9 level, white head or plant death due to disease.

[0146] Method for calculating efficacy:

[0147] Disease index (%) =∑(number of diseased plants (ears) of each level × representative value of each level) / (total number of plants (ears) surveyed × representative value of the highest level) × 100

[0148] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100

[0149] b) Investigation of white ear rate during the grain-filling stage

[0150] Each treatment area was randomly sampled at 5 points, with 100 plants at each point, to investigate the total ear number and white ear number, and the control effect was calculated.

[0151] White ear rate (%) = white ear number / total number of plants surveyed × 100

[0152] Control effect (%) = [white ear rate in the control area - white ear rate in the treatment area with the fungicide] / white ear rate in the control area × 100

[0153] 2. Test results

[0154] Table 13. Control effect of the fungicide treatment on wheat foot rot

[0155]

[0156] Note: * The values are the average of 4 replicates; ** The significance level of variance analysis is p = 0.05.

[0157] From the above table, it can be seen that during the grain-filling stage, the 10% WML-01·fludioxonil suspension agent at a high dose of 200 ml seed dressing treatment (effective ingredient 20 g) has a control effect of 91.31% on wheat foot rot, and at a low dose of 100 ml treatment (effective ingredient 10 g), the control effect on wheat foot rot is 83.99%. Both are significantly better than fludioxonil single agent seed dressing treatment (effective ingredient 10 g) and WML-01 single agent treatment (effective ingredient 20 g).

[0158] The white ear rate investigation results show that the 10% WML-01·fludioxonil suspension agent at a high dose of 200 ml treatment has a control effect of 93.96% on white ear, and at a low dose of 100 ml treatment, the control effect on white ear is 87.77%. Both are significantly better than WML-01 single agent and fludioxonil single agent treatment.

[0159] The above results show that after the WML-01 and fludioxonil are compounded, at the same dose as the fludioxonil single agent, excellent control effect on wheat foot rot is shown, and compared with the WML-01 single agent treatment, the field drug dosage is reduced while the control effect is improved.

[0160] Example 5 Field control effect of WML-01 and fludioxonil complex on rice bacterial seedling blight

[0161] 1. Materials and methods

[0162] 1.1 Test materials

[0163] 10% WML-01·fludioxonil suspension concentrate, self-made, wherein WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand milling process; 10% WML-01 suspension concentrate, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand milling process; 25g / L fludioxonil seed treatment suspension concentrate, produced by Beinong Haile (Zhuozhou) Seed Coating Agent Co., Ltd.

[0164] 1.2 Test site profile

[0165] The test was set in Zhao Yuan Village, Yaojiang Town, Zhuji City, Zhejiang Province, with clay loam soil, medium fertility, medium-early 39 rice variety, 5kg per mu of seed quantity, seedling raising and transplanting.

[0166] 1.3 Test design

[0167] Table 14 Test scheme of seed treatment on rice bacterial seedling blight

[0168]

[0169] 1.4 Investigation method and calculation

[0170] Bacterial seedling blight: randomly sample 5 points per plot in the seedbed, investigate 10cm*10cm per point, repeat 3 times, record the disease rate, and calculate the control effect.

[0171] Disease rate (%) = number of diseased plants / total number of plants investigated x 100%

[0172] Control effect (%) = (disease rate of blank control area - disease rate of treatment area) / disease rate of blank control area x 100%

[0173] 2. Test results

[0174] Table 15 Control effect of pesticide treatment on rice bacterial seedling blight

[0175]

[0176] Note: * The average value of 4 repetitions; ** The significance level of variance analysis is p=0.05.

[0177] From the above table, it can be seen that during the seedling stage, the 10% WML-01 · fludioxonil suspension agent high dose 200 ml seed dressing treatment (effective ingredient 20 g) has a prevention effect of 97.97% on rice seedling disease; 100 ml seed dressing treatment (effective ingredient 10 g) has a prevention effect of 94.09% on rice seedling disease, which is significantly better than fludioxonil single agent treatment (effective ingredient 10 g) and WML-01 single agent treatment (effective ingredient 20 g).

[0178] The above results show that after the WML-01 and fludioxonil are compounded, at the same dose as the fludioxonil single agent, the rice seedling disease shows excellent prevention effect, and compared with the WML-01 single agent treatment, the field drug dosage is reduced while the prevention effect is improved.

[0179] Example 6 Field prevention effect of WML-01 and fludioxonil compound on rice blast

[0180] 1. Materials and methods

[0181] 1.1 Test materials

[0182] 10% WML-01 · fludioxonil suspension agent, self-made, wherein WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is the mass percentage content, prepared by sand milling process; 10% WML-01 suspension agent, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is the mass percentage content, prepared by sand milling process; 30% fludioxonil suspension agent, product of Jiangxi Zhengbang Crop Protection Co., Ltd.

[0183] 1.2 Test site profile

[0184] The test was conducted in Chadi Township, Shanghang County, Longyan City, Fujian Province, and the rice variety was Changliangyou 8.

[0185] 1.3 Test design

[0186] Table 16 Test scheme of pesticide treatment on rice blast

[0187]

[0188] 1.4 Investigation method and calculation

[0189] Five-point sampling method was used, not less than 20 spikes per point, 100 spikes per plot were investigated, and the total number of spikes, the number of diseased spikes and the disease grade of each plot were recorded. The disease index and control effect were calculated.

[0190] The grading standards of panicle neck blast are as follows:

[0191] 0 grade: no disease;

[0192] 1 grade: less than 5% loss per panicle (individual branch infection);

[0193] 3 grade: 6-20% loss per panicle (about one third of branch infection);

[0194] 5 grade: 21-50% loss per panicle (panicle neck or main axis infection, half shriveled grain);

[0195] 7 grade: 51-70% loss per panicle (panicle neck infection, most shriveled grain);

[0196] 9 grade: 71-100% loss per panicle (panicle neck infection, white panicle).

[0197] The calculation method of efficacy is as follows:

[0198] Disease index (%) =∑(number of plants (panicles) of each grade × representative value of each grade) / (total number of plants (panicles) surveyed × representative value of the highest grade) × 100

[0199] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100

[0200] 2 Test results

[0201] Table 17 Effect of the fungicide treatment on rice blast

[0202]

[0203] Note: * The values are the average of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0204] From the above table, it can be seen that after 2 applications, the 10% WML-01 + fludioxonil suspension concentrate at a high dose of 200 ml (effective ingredient 20 g) and a low dose of 100 ml (effective ingredient 10 g) had a control effect on rice blast of 90.46% and 84.40%, respectively, which were significantly better than that of fludioxonil single agent treatment (effective ingredient 12 g) and WML-01 single agent treatment (effective ingredient 20 g).

[0205] The above results show that after the combination of WML-01 and fludioxonil, excellent control effect on rice blast is shown at a dose lower than that of fludioxonil single agent, and compared with WML-01 single agent treatment, the field application amount is significantly reduced while the control effect is improved.

[0206] Example 7 Field control effect of WML-01 and fludioxonil complex on sclerotinia sclerotiorum of rape

[0207] 1. Materials and methods

[0208] 1.1 Test materials

[0209] 10% WML-01·fludioxonil suspension concentrate, self-made, wherein WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is mass percentage content, prepared by sand mill process; 10% WML-01 suspension concentrate, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is mass percentage content, prepared by sand mill process; 30% fludioxonil suspension concentrate, product of Jiangxi Zhengbang Crop Protection Co., Ltd.

[0210] 1.2 Test site profile

[0211] The test was set in Da Fasi Town, Wuxue City, Huanggang City, Hubei Province, and the soil texture was loam.

[0212] Rape variety Huayouza 62, sowing amount 400g / mu.

[0213] 1.3 Test design

[0214] Table 18 Test scheme of fungicide treatment on sclerotinia sclerotiorum of rape

[0215]

[0216] 1.4 Investigation method and calculation

[0217] Five-point sampling method was used, not less than 40 plants per point, 200 plants per plot were investigated, and the number of diseased plants and disease grade of each plot were recorded. Disease index and control effect were calculated.

[0218] The grading standard is as follows:

[0219] 0 level: no disease;

[0220] 1 level: the diseased area accounts for less than 5% of the main stem surface area;

[0221] 3 level: the diseased area accounts for more than 5% and less than 15% of the main stem surface area;

[0222] 5 level: the diseased area accounts for more than 15% and less than 30% of the main stem surface area;

[0223] 7 level: the diseased area accounts for more than 30% and less than 50% of the main stem surface area;

[0224] 9 grade: the disease area accounts for more than 50% of the main stem surface area.

[0225] Pharmacodynamic calculation method:

[0226] Disease index (%) =∑(number of plants at each level x representative value at each level) / (total number of plants surveyed x representative value of the highest level) x 100

[0227] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area x 100

[0228] 2 Test results

[0229] Table 19 Effect of fungicide treatment on Sclerotinia sclerotiorum of rape

[0230]

[0231] Note: * The average of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0232] From the above table, it can be seen that after 2 applications, the 10% WML-01 + fludioxonil suspension concentrate at a high dose of 150 ml (effective ingredient 15 g) has a control effect of 92.09% on Sclerotinia sclerotiorum of rape, which is significantly better than fludioxonil single agent treatment (effective ingredient 12 g) and WML-01 single agent treatment (effective ingredient 20 g). The low dose of 75 ml (effective ingredient 7.5 g) has a control effect of 85.20% on Sclerotinia sclerotiorum of rape, which is equivalent to fludioxonil single agent treatment and better than WML-01 single agent treatment.

[0233] The above results show that after compounding WML-01 and fludioxonil, the compound has a control effect on Sclerotinia sclerotiorum of rape at a significantly lower dose than fludioxonil single agent, and relative to WML-01 single agent treatment, the field application amount is significantly reduced while the control effect is improved.

[0234] Example 8 Field control effect of WML-01 and fludioxonil compound on pepper anthracnose

[0235] 1 Materials and methods

[0236] 1.1 Test material

[0237] 10% WML-01 + fludioxonil SC, self-made, containing WML-01 5%, fludioxonil 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % by mass, prepared by sand mill process; 10% WML-01 SC, self-made, containing WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % by mass, prepared by sand mill process; 30% fludioxonil SC, product of Jiangxi Zhengbang Crop Protection Co., Ltd.

[0238] 1.2 Test site profile

[0239] The test was conducted in Daxin Town, Fugou County, Zhoukou City, Henan Province, with soil texture being loam, and pepper variety being Mibao 19.

[0240] 1.3 Test design

[0241] Table 20 Test scheme of fungicide treatment on pepper anthracnose

[0242]

[0243] 1.4 Investigation method and calculation

[0244] 5 points were sampled in each plot, and 20 fruits were investigated in each point, with the percentage of lesion area to the whole fruit area as the grading standard.

[0245] Grading method:

[0246] 0 level: no lesion;

[0247] 1 level: lesion area accounting for less than 5% of the whole fruit area;

[0248] 3 level: lesion area accounting for more than or equal to 5% and less than 10% of the whole fruit area;

[0249] 5 level: lesion area accounting for more than or equal to 10% and less than 25% of the whole fruit area;

[0250] 7 level: lesion area accounting for more than or equal to 25% and less than 50% of the whole fruit area;

[0251] 9 level: lesion area accounting for more than or equal to 50% of the whole fruit area, or fruit drop.

[0252] Pharmacodynamic calculation method:

[0253] Disease index (%) = ∑ (number of fruits at each level × representative value at each level) / (total number of investigated fruits × representative value at the highest level) × 100

[0254] Control effect (%) = (disease index of control area after treatment - disease index of treatment area after treatment) / disease index of control area after treatment x 100

[0255] 2 Test results

[0256] Table 21. Control effect of the agent treatment on pepper anthracnose

[0257]

[0258] Note: * The average of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0259] From the above table, it can be seen that after 2 times of spraying, the control effect of 10% WML-01 · fludioxonil suspension concentrate 200 ml treatment (effective ingredient 20 g) and 100 ml treatment (effective ingredient 10 g) on pepper anthracnose is 90.58% and 86.67% respectively, which is significantly better than that of fludioxonil single agent treatment (effective ingredient 9 g) and WML-01 single agent treatment (effective ingredient 20 g).

[0260] The above results show that after the compounding of WML-01 and fludioxonil, at about the same dose as fludioxonil single agent, excellent control effect on pepper anthracnose is shown, and compared with WML-01 single agent treatment, when the dose is equivalent, more excellent control effect is shown, and when the dose is significantly reduced, the control effect is also higher than that of WML-01 single agent treatment.

[0261] Although the present application has been described in detail in the foregoing with general description and specific embodiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application claimed.

Claims

1. An agricultural fungicidal composition, characterized by, The active ingredient of the fungicidal composition comprises WML-01 and fludioxonil, and the mass ratio of the WML-01 and the fludioxonil is 10:1-1:10; the structural formula of the WML-01 is shown in formula I: 。 2. The agricultural fungicidal composition according to claim 1, characterized by The mass ratio of the WML-01 and the fludioxonil is 10:1-1:

1.

3. The agricultural fungicidal composition according to claim 1, wherein The mass ratio of the WML-01 and the fludioxonil is 3:1-1:

10.

4. A bactericidal preparation, characterized by, The fungicidal preparation comprises the agricultural fungicidal composition of claim 1.

5. The bactericidal preparation according to claim 4, characterized in that, The dosage form of the fungicidal preparation is seed coating agent, suspension agent, water emulsion, microemulsion, emulsifiable concentrate, microcapsule suspension preparation, nano preparation, granule, wettable powder or water dispersible granule.

6. The sterilizing preparation according to claim 4, characterized in that, The components of the fungicidal preparation further comprise auxiliary agents for assisting the agricultural fungicidal composition, and the auxiliary agents are xanthan gum, wetting agent, dispersing agent, defoaming agent, antifreezing agent or warning color.

7. The bactericidal preparation according to claim 6, characterized in that, The wetting agent is alkyl phenol polyoxyethylene ether or alkyl naphthalene sulfonate; the dispersing agent is sodium salt of alkyl naphthalene sulfonate polycondensate or sulfonate of alkyl naphthalene sulfonate polycondensate; the defoaming agent is n-octanol or organic silicon; the antifreezing agent is ethylene glycol; and the warning color is basic roseine, gold red or fast peach red.

8. The application of the agricultural fungicide composition according to any one of claims 1-3 or the fungicide preparation according to any one of claims 4-7 in the control of plant diseases, wherein the plant disease is caused by Fusarium oxysporum. Fusarium fujikuroi The rice seedling blight caused by the fungicide composition or fungicide preparation has a mass ratio of WML-01 to fludioxonil of 10:1 to 1:

1. or the plant disease is caused by Fusarium pseudograminearum Fusarium pseudograminearum wheat seedling blight, the mass ratio of WML-01 and fludioxonil in the fungicidal composition or the fungicidal preparation is 3:1~1:10; or the plant disease is wheat scab caused by Fusarium graminearum Fusarium graminearum WML-01 and fludioxonil in the fungicidal composition or the fungicidal preparation have a mass ratio of 3:1 to 1:

10. or the plant disease is soybean root rot caused by Fusarium oxysporum Fusariumoxysporum WML-01 and fludioxonil in the fungicidal composition or the fungicidal preparation are in a mass ratio of 1:1~1:

10. or the plant disease is rice blast caused by Magnaporthe oryzae Pyricularia oryzae WML-01 and fludioxonil in the fungicidal composition or the fungicidal preparation are in a mass ratio of 3:1~1:1; or the plant disease is caused by Colletotrichum gloeosporioides Colletotrichumgloeosporioides pepper anthracnose, and the mass ratio of WML-01 to fludioxonil in the fungicidal composition or the fungicidal preparation is 5:1 to 1:

1. or the plant disease is caused by Sclerotinia sclerotiorum Sclerotinia sclerotiorum caused by Sclerotinia sclerotiorum, and the mass ratio of WML-01 to fludioxonil in the fungicidal composition or the fungicidal preparation is 2:1-1:10.

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