Traditional Chinese medicine composition for treating qi stagnation and collateral arthralgia type depression

Abstract

The invention provides a traditional Chinese medicine composition for treating qi stagnation and collateral arthralgia type depression. The composition is mainly prepared from 25 to 400 parts of radix curcumae, 30 to 500 parts of radix angelicae sinensis, 30 to 600 parts of rhizoma chuanxiong, 30 to 150 parts of radix paeoniae alba, 30 to 150 parts of cassia twig and 30 to 150 parts of poria cocos. Animal experiments show that the traditional Chinese medicine composition can effectively improve depression-like symptoms of mice with depression, the anti-depression effect of the traditional Chinese medicine composition is equivalent to that of a positive drug fluoxetine, and no obvious adverse drug reaction exists. In addition, the traditional Chinese medicine composition also has obvious curative effects of regulating qi and dredging collaterals, and the positive drug fluoxetine has no response effect. The traditional Chinese medicine composition has the advantages of simple formula, easily available raw materials, strong adaptation specificity, obvious curative effect and simple preparation process, and is suitable for industrial production.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically relating to a traditional Chinese medicine preparation for treating depression of the Qi stagnation and meridian obstruction type. Background Technology

[0002] Depression is a prevalent mental disorder worldwide, clinically characterized by persistent low mood, loss of interest, cognitive impairment, and physical symptoms. World Health Organization data shows that over 300 million people worldwide suffer from depression, and the incidence rate continues to rise. Modern medicine believes that the pathogenesis of depression involves the interaction of multiple factors, including genetics, environment, neurotransmitter imbalances (such as abnormalities in the 5-HT, DA, and NE systems), HPA axis dysfunction, and neuroinflammation. Current Western medicine treatment primarily relies on antidepressants (such as SSRIs and SNRIs), but these have limitations such as slow onset of action, insufficient response in 30%-50% of patients, withdrawal syndrome, sexual dysfunction, and weight gain, prompting the medical community to explore safer and more effective alternative therapies. Traditional Chinese medicine (TCM) has a long history and unique theoretical system in the prevention and treatment of depression. TCM categorizes depression under "Yu Zheng," "Bai He Bing," and "Zang Zao," believing that its core pathogenesis lies in emotional imbalance leading to liver dysfunction, qi stagnation, and consequently, disharmony of qi and blood, and dysfunction of the internal organs. Unlike most commonly used antidepressants that target the nervous system, Traditional Chinese Medicine (TCM) believes that different TCM formulas should be used to treat patients with depression based on their specific causes and mechanisms, achieving holistic regulation at multiple levels and targeting multiple points. This approach offers high safety and addresses both the symptoms and the root cause. Therefore, developing effective TCM antidepressant formulas can provide new ideas and methods for the prevention and treatment of depression. Summary of the Invention

[0003] This invention provides a traditional Chinese medicine composition for treating depression of the Qi stagnation and collaterals type. The composition is mainly composed of the following traditional Chinese medicines: 25-400 parts of Curcuma longa, 30-500 parts of Angelica sinensis, 30-600 parts of Ligusticum chuanxiong, 30-150 parts of Paeonia lactiflora, 30-150 parts of Cinnamomum cassia, and 30-150 parts of Poria cocos.

[0004] The traditional Chinese medicine composition may also include 20-200 parts of yam.

[0005] The preferred formulation of this traditional Chinese medicine composition is as follows: 80-400 parts of turmeric, 150-500 parts of angelica sinensis, 30-200 parts of chuanxiong rhizome, 80-150 parts of white peony root, 150 parts of cinnamon twig, 30-150 parts of poria cocos, and 80-200 parts of yam.

[0006] One of the specially selected formulas is: 400 parts turmeric, 500 parts angelica, 30 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 200 parts yam.

[0007] The second specially selected formula is: 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 30 parts poria, and 80 parts yam.

[0008] The third specially selected formula is: 80 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 120 parts yam.

[0009] The fourth specially selected formula is: 120 parts of turmeric, 100 parts of angelica, 160 parts of chuanxiong, 80 parts of white peony root, 150 parts of cinnamon twig, and 80 parts of poria.

[0010] In the above-mentioned formula, turmeric can be replaced by bupleurum; chuanxiong can be replaced by one or two of cyperus and tangerine peel; white peony can be replaced by one or two of angelica and fleeceflower root; cinnamon twig can be replaced by polygala; and poria can be replaced by one or two of poria cocos, stir-fried yam, stir-fried atractylodes macrocephala, and stir-fried white hyacinth bean.

[0011] Wash the above-mentioned medicinal materials, mix them in the specified proportions, add 8-10 times the total weight of the medicinal materials in water and soak for 30 minutes. Then, decoct each time to 400 ml, and combine the two decoctions. Concentrate the decoction to obtain a concentrated extract. Then, freeze-dry the extract to obtain a freeze-dried powder.

[0012] According to conventional pharmaceutical manufacturing processes, the above raw materials can be prepared into commonly used clinical drug dosage forms, such as pills, tablets, granules, oral liquids, capsules, ointments, emulsions, chewable tablets, and injections.

[0013] The traditional Chinese medicine composition of this invention has the advantages of soothing the liver and regulating qi, promoting blood circulation and unblocking collaterals, relieving depression and calming the mind. Turmeric is the chief ingredient, which soothes the liver and relieves depression, regulates qi, and calms the mind. Angelica sinensis and Ligusticum chuanxiong are the assistant ingredients, which nourish blood, promote blood circulation, and relieve depression. White peony root, cinnamon twig, and Poria cocos are added to nourish blood, soften the liver, warm and unblock the meridians, promote diuresis, and strengthen the spleen, collectively exerting the effects of promoting qi circulation, relieving depression, nourishing yin, and calming the mind. Animal experiments show that this invention can effectively improve depressive-like symptoms in mice with depression, with an antidepressant effect comparable to the positive control drug fluoxetine, and no obvious adverse drug reactions. Furthermore, this traditional Chinese medicine composition also has significant effects in regulating qi and unblocking collaterals, while the positive control drug fluoxetine has no corresponding effect. This traditional Chinese medicine composition has a simple formulation, readily available raw materials, strong indication specificity, significant efficacy, and a simple preparation process, making it suitable for industrial production. Attached Figure Description

[0014] Figure 1This figure illustrates the effects of different doses of this composition on depressive-like behavior in a CSDS-induced depression mouse model. Subfigure A shows the weight changes in CSDS-induced depression mice during drug administration. Subfigure B shows the standardized statistical results of sucrose preference in CSDS-induced depression mice. Subfigure C shows the standardized statistical results of the percentage of immobility during the forced swimming test (FST) in CSDS-induced depression mice. (CON: blank control; CSDS: chronic mild unpredictable stress; Fluoxetine: fluoxetine, a positive drug for depression; SPT: sucrose preference test; FST: forced swimming test.)

[0015] Figure 2 This is a diagram illustrating the effect of this composition on the qi deficiency phenotype in CSDS model animals; Subplot A: Statistical chart of the ratio of open-arm dwell time in the elevated cruciate maze for CSDS mice; Subplot B: Statistical chart of attack latency in CSDS mice; Subplot C: Statistical chart of the number of attacks in CSDS mice; Figure 2 The new compound in this context refers to this composition.

[0016] Figure 3 This is a graphic illustration of the effect of this composition on the paralytic phenotype in CSDS model animals. Sub-figure A: Representative laser speckle images of cerebral perfusion in each group; Sub-figure B: Representative laser speckle images of blood flow perfusion in the right hind limb in each group; Sub-figure C: Statistical results of cerebral perfusion in each group; Sub-figure D: Statistical results of perfusion in the right hind paw in each group; Sub-figure E: Statistical results of reactive hyperemia time in the right hind paw in each group (PU: perfusion unit).

[0017] Figure 4 The illustration shows the effect of the compositions 1 to 9 on reducing the sugar water preference rate in CSDS model animals. The serial numbers of the examples correspond to the composition numbers in the figure.

[0018] Figure 5 The illustration shows the effect of formulations 10 to 18 on reducing the sugar water preference rate in CSDS model animals. The serial numbers of the examples correspond to the formulation numbers in the figure.

[0019] Figure 6 The illustration shows the effect of formulations 19 to 27 on reducing the sugar water preference rate in CSDS model animals. The serial numbers of the examples correspond to the formulation numbers in the figure.

[0020] Figure 7 The illustration shows the effect of the compositions 28 to 31 on reducing the sugar water preference rate in CSDS model animals. The serial numbers of the examples correspond to the composition numbers in the figure. Detailed Implementation

[0021] The present invention will be further described below with reference to specific embodiments.

[0022] I. Validation of the efficacy of the combination of the present invention and determination of the optimal dosage.

[0023] 1. Experimental Methods

[0024] 1.1 Establishment and grouping of experimental animal models

[0025] 120 SPF-grade C57BL / 6J male mice, 7 weeks old, were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. The animal license number is SCXK(Beijing)2021-0006. The animals were housed at a temperature of 25±2°C and a humidity of 55±5%, with a 12-hour light-dark cycle. During the experiment, the animals had free access to food and water.

[0026] After one week of adaptive feeding, an open field test was conducted on all animals to exclude those with abnormal mental states or impaired motor abilities, ensuring the smooth progress of subsequent experiments.

[0027] 1.2 Chronic social stress-induced depression model (CSDS)

[0028] C57BL / 6J mice were selected. The mice in the model group were exposed to different CD-1 aggressive mice for 10 minutes every day. Within 24 hours after being attacked, the two types of mice were separated by a transparent plastic plate with holes, allowing the mice to observe and smell the CD-1 mice while in non-contact isolation. The stress lasted for 10 days, and each mouse was exposed to different CD-1 mice. Twenty-four hours after the last stress, a social avoidance test was conducted to screen susceptible mice for subsequent experiments.

[0029] 1.3 Preparation of drugs

[0030] The original drug of the preparation in this invention is a yellowish-brown traditional Chinese medicine freeze-dried powder, with the specific ratio as follows: 120 parts of Curcuma aromatica Salisb., 100 parts of Angelica sinensis (Oliv.) Diels, 160 parts of Ligusticum chuanxiong Hort., 80 parts of Paeonia lactiflora Pall., 150 parts of Cinnamomum cassia Presl, and 80 parts of Poria cocos (Schw.) Wolf. According to the dosage requirements, the freeze-dried powder of the preparation in this invention was dissolved in pure water to prepare high, medium, and low doses. Fluoxetine was dissolved in pure water to prepare a 20mg / ml solution as the positive drug. The new compound was administered at doses of 20.4g crude drug / kg (high dose), 10.2g crude drug / kg (medium dose), and 5.1g crude drug / kg (low dose), and fluoxetine was used as the positive drug at 20mg / kg. The blank group was given the same volume of pure water.

[0031] 1.4 Drug treatment

[0032] After modeling, the model mice were randomly divided into 5 groups of 12 mice each: CSDS model group, CSDS + low-dose new formulation group, CSDS + medium-dose new formulation group, CSDS + high-dose new formulation group, and CSDS + fluoxetine group. The blank control group and CSDS model group were administered pure water by gavage once daily; the CSDS + low-dose, medium-dose, and high-dose new formulation groups were administered a lyophilized aqueous solution of the new formulation by gavage once daily; and the CSDS + fluoxetine group was administered a fluoxetine aqueous solution by gavage once daily. Behavioral experiments were conducted 14 days after administration.

[0033] 1.5 Evaluation of Depressive-like Phenotype (see Table 1 for details)

[0034] Table 1 Evaluation indicators of depressive behavior in mice

[0035]

[0036] 1.6 Statistical Methods

[0037] Statistical analysis was performed using SPSS 19.0 software. Experimental results are expressed as mean ± standard error (Mean ± SEM). One-way ANOVA was used to test the significance of the data. * indicates p < 0.05, and ** indicates p < 0.01. The final results were imported into Prism 9.3 for plotting.

[0038] 2. Experimental Results

[0039] Depressive-like behaviors in CSDS mice were assessed using body weight, sucrose water test, and forced swimming test.

[0040] Mouse weight change

[0041] Fourteen days after administration, the CSDS group mice showed a significant decrease in body weight compared to the blank control group. However, after drug treatment, mice in the low, medium, and high doses of the new compound and the positive control group (fluoxetine) showed an increase in body weight, demonstrating that the new compound can improve the weight loss in CSDS mice, and its efficacy is comparable to that of the positive control group (see...). Figure 1 A).

[0042] Sugar water preference experiment

[0043] The results of saccharide preference analysis showed that, compared with the blank control group, the saccharide preference rate of mice in the CSDS model group was significantly reduced; while the saccharide preference rate of mice in the high-dose group of the new compound was significantly increased, and the low-dose group had no effect on improving the saccharide preference of the model mice. This indicates that the anhedonia phenotype of CSDS model mice was improved after taking the new compound, and this therapeutic effect was comparable to that of fluoxetine (see...). Figure 1 B).

[0044] Forced swimming experiment

[0045] Forced swimming test results showed that, compared with the blank control group, the immobility time of CSDS model mice was significantly reduced; the immobility time of mice in the high-dose group of the new compound was significantly reduced, while the low-dose group had no effect on improving the immobility time of model mice. This indicates that the high-dose group of the new compound can have the same effect as fluoxetine in improving the despair behavior of CSDS model mice, and its therapeutic effect is comparable to that of fluoxetine (see...). Figure 1 C).

[0046] Based on the above, both high and medium doses of the new compound significantly improved depressive-like behavior in CSDS mice. Secondly, considering the difficulty of administering high doses of the new compound via gavage, this study subsequently selected a medium dose of the new compound as the experimental study dose.

[0047] II. Detection of pneumostasis-like behavior in mice using the composition of the present invention.

[0048] 1. Materials and Methods

[0049] The experimental animals, experimental instruments, experimental reagents, establishment and grouping of experimental animal models, drug preparation, drug treatment, and statistical methods are the same as above.

[0050] 2. Determination of indicators for detecting gas stagnation in mice:

[0051] By retrieving 3766 clinical studies related to depression and 2843 clinical studies related to qi stagnation up to 2024, inclusion and exclusion criteria were established for these studies. Studies that did not meet the inclusion criteria were removed. Data extraction and standardization were then performed on the collected data. Standardized data resulted in a database of depressive symptoms / signs and a database of qi stagnation symptoms / signs. Based on similar symptom_ids in both databases, common symptoms were summarized, resulting in 296 common symptoms of depression and qi stagnation. After screening, the following indicators were ultimately established as the detection indicators for "qi stagnation" in mice (see Table 2).

[0052] Table 2. Indicators of gas stagnation in mice

[0053]

[0054] 3. Experimental results

[0055] Here, we use the elevated cross maze test to assess the "irritability" state of mice and aggressive behavior to assess the "anger" state of mice.

[0056] 3.1 Evaluation of "Agitation" Symptoms in CSDS Mice

[0057] Compared with the blank control group, the CSDS group mice had a shorter open-arm dwell time; compared with the CSDS group mice, the new compound group mice had an increased open-arm dwell time, while no improvement was observed after administration of fluoxetine (see...). Figure 2 A) The elevated cross maze experiment proved that the new compound could improve the "irritability" symptoms in mice.

[0058] 3.2 Evaluation of the "irritable" state in CSDS mice

[0059] Compared with normal control mice, mice in the CSDS group had a significantly shorter attack latency and a higher number of attacks; compared with mice in the model group, mice in the new compound group had a significantly longer attack latency and a lower number of attacks (see...). Figure 2 (BC). Aggressive behavior testing in mice demonstrated that CSDS mice exhibited an "irritable" phenotype, which could be improved after treatment with the new compound, but fluoxetine did not improve the above symptoms.

[0060] III. Detection of paralytic-like behavior in mice using the composition of the present invention.

[0061] 1. Materials and Methods

[0062] The experimental animals, experimental instruments, experimental reagents, establishment and grouping of experimental animal models, drug preparation, drug treatment, and statistical methods are the same as above.

[0063] 1.2 Determination of Indicators for Detecting Collateral Pain in Mice

[0064] By retrieving 3766 clinical articles related to depression and 3341 clinical articles related to blood stasis / collateral obstruction from 2024 onwards, inclusion and exclusion criteria were established for these articles. Articles that did not meet the inclusion criteria were removed. Data extraction and standardization were then performed on the collected articles. Standardized data resulted in a database of depressive symptoms / signs and a database of qi deficiency symptoms / signs. Based on similar symptom_ids in these two databases, common symptoms were summarized, resulting in 156 common symptoms of depression and collateral obstruction. Furthermore, based on the characteristics of small animal detection indicators, the following indicators were ultimately established as detection indicators for "collateral obstruction" in mice (see Table 3).

[0065] Table 3 Indicators for detecting paralysis in mice

[0066]

[0067] Spearman correlation analysis was performed on the above symptom assessment indicators within a variable range to explore the potential association between them. The results showed that in the CSDS model, microvascular assessment variables (meningeal microvascular perfusion, right hind paw microvascular perfusion, and reactive hyperemia time) were significantly correlated with depressive behaviors (sucrose preference and forced swimming immobility time). Therefore, meningeal microvascular perfusion, right hind paw microvascular perfusion, and reactive hyperemia time were selected as evaluation indicators.

[0068] 2. Experimental Results

[0069] This study evaluated the therapeutic effect of the new compound on microcirculatory dysfunction in CSDS model mice using laser Doppler experiments (see...). Figure 3 (AB). The results showed that, compared with the blank control group, the CSDS model group mice had significantly reduced cerebral and paw blood perfusion and prolonged reactive hyperemia time; after treatment with the new compound, the cerebral and paw blood perfusion of mice significantly increased and the reactive hyperemia time was shortened (see AB). Figure 3 (CE). This study demonstrated that the microcirculatory damage in the whole body and brain of CSDS model mice was restored after administration of the new compound, but mice treated with fluoxetine did not show significant changes in cerebral blood flow perfusion, paw blood flow perfusion, or reactive hyperemia.

[0070] IV. Verification of the antidepressant effect of this composition with different ratios

[0071] Example 1:

[0072] 25 parts turmeric, 500 parts angelica, 500 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, and 150 parts poria.

[0073] The drug preparation and testing methods are the same as above.

[0074] The sucrose preference test has become the most commonly used behavioral detection method for depression in the CSDS model due to its direct association with core depressive symptoms, ease of operation, high sensitivity, and well-defined neural mechanisms. Therefore, the efficacy of this traditional Chinese medicine composition was evaluated using the sucrose preference test, with the specific method described above.

[0075] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0076] Example 2:

[0077] 400 parts of turmeric, 30 parts of angelica, 600 parts of chuanxiong, 150 parts of white peony root, 150 parts of cinnamon twig, and 150 parts of poria cocos.

[0078] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0079] Example 3:

[0080] 400 parts of turmeric, 500 parts of angelica, 30 parts of chuanxiong, 150 parts of white peony root, 150 parts of cinnamon twig, and 150 parts of poria cocos.

[0081] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0082] Example 4:

[0083] 400 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 30 parts white peony root, 30 parts cinnamon twig, and 150 parts poria.

[0084] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0085] Example 5:

[0086] 400 parts of turmeric, 500 parts of angelica, 600 parts of chuanxiong, 150 parts of white peony root, 150 parts of cinnamon twig, and 30 parts of poria cocos.

[0087] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 son Figure 5 .

[0088] Example 6:

[0089] 400 parts of turmeric, 500 parts of angelica, 600 parts of chuanxiong, 150 parts of white peony root, 150 parts of cinnamon twig, and 150 parts of poria cocos.

[0090] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0091] Example 7:

[0092] 25 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, and 150 parts poria.

[0093] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0094] Example 8:

[0095] 400 parts of turmeric, 30 parts of angelica, 600 parts of chuanxiong, 150 parts of white peony root, 150 parts of cinnamon twig, and 150 parts of poria cocos.

[0096] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0097] Example 9:

[0098] 400 parts turmeric, 30 parts angelica, 600 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 150 parts stir-fried yam.

[0099] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0100] Example 10:

[0101] 400 parts turmeric, 500 parts angelica, 30 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 200 parts stir-fried yam.

[0102] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0103] Example 11:

[0104] 400 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 30 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 200 parts stir-fried yam.

[0105] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0106] Example 12:

[0107] 400 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 30 parts poria cocos, and 200 parts stir-fried yam.

[0108] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0109] Example 13:

[0110] 400 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 100 parts stir-fried yam.

[0111] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0112] Example 14:

[0113] 400 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 50 parts stir-fried yam.

[0114] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0115] Example 15:

[0116] 400 parts turmeric, 500 parts angelica, 600 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 20 parts stir-fried yam.

[0117] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0118] Example 16:

[0119] 60 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, and 150 parts poria.

[0120] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0121] Example 17:

[0122] 150 parts turmeric, 80 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 100 parts poria cocos, and 150 parts stir-fried yam.

[0123] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0124] Example 18:

[0125] 150 parts turmeric, 180 parts angelica, 100 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 100 parts poria cocos, and 150 parts stir-fried yam.

[0126] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0127] Example 19:

[0128] 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 30 parts poria cocos, and 150 parts stir-fried yam.

[0129] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0130] Example 20:

[0131] 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 30 parts poria cocos, and 80 parts stir-fried yam.

[0132] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0133] Example 21:

[0134] 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 100 parts poria cocos, and 80 parts stir-fried yam.

[0135] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0136] Example 22:

[0137] 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 100 parts poria cocos, and 80 parts stir-fried yam.

[0138] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0139] Example 23:

[0140] 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 100 parts poria cocos, and 20 parts stir-fried yam.

[0141] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0142] Example 24:

[0143] 80 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 120 parts stir-fried yam.

[0144] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0145] Example 25:

[0146] 120 parts turmeric, 100 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 120 parts stir-fried yam.

[0147] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0148] Example 26:

[0149] 120 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 40 parts poria cocos, and 120 parts stir-fried yam.

[0150] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0151] Example 27:

[0152] 120 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 50 parts stir-fried yam.

[0153] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0154] Example 28:

[0155] 120 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 30 parts stir-fried yam.

[0156] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 7 .

[0157] Example 29:

[0158] 100 parts turmeric, 120 parts angelica, 130 parts chuanxiong, 65 parts white peony root, 150 parts cinnamon twig, 60 parts poria cocos, and 90 parts stir-fried yam.

[0159] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 7 .

[0160] Example 30:

[0161] 100 parts turmeric, 120 parts angelica, 130 parts chuanxiong, 65 parts white peony root, 150 parts cinnamon twig, 60 parts poria cocos, and 30 parts stir-fried yam.

[0162] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 7 .

[0163] Example 31:

[0164] 120 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 30 parts stir-fried yam.

[0165] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CSDS and reducing sucrose preference rate. (See results below.) Figure 7 .

Claims

1. A traditional Chinese medicine composition for treating depression of the Qi stagnation and meridian obstruction type, characterized in that, By weight, the traditional Chinese medicine composition mainly consists of the following traditional Chinese medicines: Turmeric 25-400 parts, Angelica sinensis 30-500 parts, Ligusticum chuanxiong 30-600 parts, Paeonia lactiflora 30-150 parts, Cinnamomum cassia 30-150 parts, Poria cocos 30-150 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition also includes 20-200 parts of yam.

3. The traditional Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: Turmeric 80-400 parts, Angelica sinensis 150-500 parts, Ligusticum chuanxiong 30-200 parts, Paeonia lactiflora 80-150 parts, Cinnamomum cassia 150 parts, Poria cocos 30-150 parts, Dioscorea opposita 80-200 parts.

4. The traditional Chinese medicine composition according to claim 3, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: 400 parts turmeric, 500 parts angelica, 30 parts chuanxiong, 150 parts white peony root, 150 parts cinnamon twig, 150 parts poria cocos, and 200 parts yam.

5. The traditional Chinese medicine composition as described in claim 3, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: 150 parts turmeric, 180 parts angelica, 200 parts chuanxiong, 100 parts white peony root, 150 parts cinnamon twig, 30 parts poria, and 80 parts yam.

6. The traditional Chinese medicine composition according to claim 3, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: 80 parts turmeric, 150 parts angelica, 160 parts chuanxiong, 80 parts white peony root, 150 parts cinnamon twig, 80 parts poria cocos, and 120 parts yam.

7. The traditional Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: 120 parts of turmeric, 100 parts of angelica, 160 parts of chuanxiong, 80 parts of white peony root, 150 parts of cinnamon twig, and 80 parts of poria cocos.

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