Biological experiment method and device

Abstract

The invention discloses a biological experiment method and device, and the method comprises the steps: enabling two reaction slides to get close to each other at a first speed V1, and switching to a second speed V2 to continue to get close to each other when the distance reaches a preset critical distance L1; observing whether bubbles appear in a target area; if yes, controlling the at least one reaction slide to be far away from each other at a third speed V3; then controlling the at least one reaction slide to be close to each other at the second speed V2; and circulating until no bubble exists in the target area. Bubbles in a target area are directionally repelled or eliminated by dynamically lifting the reaction slide and controlling the speed in stages, so that the experiment efficiency and accuracy are improved, the manual operation difficulty and the sample safety are reduced, the experiment process is ensured to be smoothly carried out, and an effective data result is obtained. The bubble removal speed is higher, and the success rate is higher; the method is more friendly to a sample attached to the reaction slide. And the compatible surface is wider, and different types of bubbles can be well removed.

Description

Technical Field

[0001] This invention belongs to the field of biological experimental equipment technology, and in particular relates to a biological experimental method and apparatus. Background Technology

[0002] In biological sample preparation (such as cell staining, tissue section mounting, and biochip information transfer), attaching coverslips to samples or reagents on slides is a crucial step. However, due to liquid surface tension, tissue surface unevenness, and improper operator handling, air bubbles are easily generated when attaching reagent samples to slides. These bubbles interfere with optical observation, thus affecting the experimental reaction process; if bubbles appear in the biological reaction area, they can lead to inaccurate experimental data. Traditional methods rely on manually tilting the coverslip to remove air bubbles, which is time-consuming and has the following drawbacks: 1. Low operational efficiency and high requirements for operator experience; 2. Uneven manual force can easily damage samples or break the coverslip.

[0003] Chinese patent document CN118707122B discloses a laboratory biological tissue block detection device and method, including a base plate with a drive motor mounted on top of the base plate, and further including: a turntable rotatably disposed above the base plate; a detection component disposed on top of the base plate for detecting biological tissue blocks; a dripping component disposed on top of the base plate for adding physiological saline to the biological tissue block before detection; a feeding component disposed on top of the base plate; and an air-removing component disposed on top of the base plate. The device automatically and slightly rotates the coverslip to eliminate air bubbles between the slide, biological tissue, and coverslip, preventing air bubbles from affecting the detection results during electron microscopy detection of biological tissue, thereby improving the detection efficiency of the device.

[0004] The basic principle of the aforementioned patented solution is that the relative rotation of two glass slides generates a shear force on the liquid film, breaking the surface tension between the bubble and the liquid film, thus releasing the bubble. In fluid mechanics, this is known as Couette flow. The shear force is calculated using Newton's law of viscosity, with the formula F = (μ*A*U) / h. That is, the magnitude of the shear force F is directly proportional to the relative rotational speed U of the two glass slides and inversely proportional to the thickness h of the liquid film.

[0005] In practical applications, the liquid film thickness h is extremely small. For example, the liquid film thickness between a microscope coverslip and a slide is typically on the order of 0.1 mm or even smaller. This means that even a small change in the relative rotation speed U can lead to a large change in the shear force F. Excessive shear force F can cause the sample attached to the slide to shift or even be damaged, while insufficient shear force F is insufficient to drive the bubble. Therefore, this method requires highly precise control and adjustment of the relative rotation speed U, necessitating complex mechanical structures and control systems.

[0006] In addition, droplets also have various uncertainties in practical applications. Although the above-mentioned patented solution can remove tiny bubbles in principle (the bubble only contacts one glass slide), for larger bubbles (the two ends of the bubble contact two glass slides respectively), the relative rotation of the two glass slides cannot allow the liquid to pass through the top of the larger bubble, but will only go around the larger bubble. Therefore, the above-mentioned patented solution cannot remove larger bubbles and has certain limitations in application. Summary of the Invention

[0007] To overcome the technical problems of air bubbles generated during the attachment of two glass slides in existing technologies, which are difficult to remove manually and whose automated tools are complex in mechanical structure and control system, costly, and unable to remove larger bubbles, this invention aims to provide a biological experimental method and apparatus. Through staged speed switching and dynamic displacement control, the distance between two reaction glass slides is repeatedly changed. This not only crushes bubbles but also generates a pressure gradient and velocity field in the fluid. According to the Reynolds equation or lubrication approximation theory, reciprocating flow occurs between the center and edge of the liquid, causing bubbles to flow outwards and break up. This method can remove both small and large bubbles, has a wide range of applications, and offers faster and more efficient removal speeds.

[0008] To achieve the above objectives, the present invention employs the following technical solution: a biological experimental method, comprising the following steps:

[0009] S1. Prepare two reaction slides, install at least one of the reaction slides on the clamping mechanism, add a reaction liquid droplet to the target area of ​​at least one of the reaction slides to form a droplet with a diameter of D, and maintain an initial distance L0 between the two reaction slides.

[0010] S2 causes the two reaction slides to approach each other at a first speed V1. When the distance between the two reaction slides reaches a preset critical distance L1, the speed is switched to a second speed V2 to continue approaching each other. The second speed V2 is less than the first speed V1.

[0011] During the process of the two reaction slides approaching each other at the second speed V2 in S3, the droplet is gradually compressed and expands between the two reaction slides to form a liquid film. The liquid film is observed in real time to see if there are bubbles in the target area: if there are no bubbles in the target area, step S7 is executed directly; if there are bubbles in the target area, the distance between the two reaction slides is recorded as L2, and steps S4, S5, S6, and S7 are executed in sequence.

[0012] S4 controls at least one of the reaction slides to move the two reaction slides away from each other at a third speed V3 until the distance between the two reaction slides reaches the preset critical distance L1. The third speed V3 is greater than the second speed V2. During this period, the liquid film is subjected to shear force and is crushed or squeezed out of the target area. S5 controls at least one of the reaction slides to move the two reaction slides closer to each other at the second speed V2 until the liquid film completely covers the target area. At this time, the distance between the two reaction slides is L3, where L... min <L3≤L2, L min This is the minimum distance that the two reaction slides need to achieve;

[0013] S6 repeats steps S4 and S5 until no more bubbles are present in the target area;

[0014] S7 continues to press and move closer together, the liquid film spreading evenly between the two reaction slides until the two reaction slides reach their minimum distance L. min The two reaction slides stop moving, completing the attachment process.

[0015] Specifically, the first velocity V1 is greater than or equal to 0.1 mm / s and less than or equal to 100 mm / s; the second velocity V2 is greater than or equal to 0.01 mm / s and less than or equal to 1 mm / s; and the third velocity V3 is greater than or equal to 0.1 mm / s and less than or equal to 10 mm / s.

[0016] Specifically, the initial spacing L0 is greater than or equal to 0.01 mm and less than or equal to 100 mm; the preset critical distance L1 is greater than or equal to 0.1 mm and less than or equal to 3 mm; and the L3 is greater than or equal to 0.1 mm and less than or equal to 5 mm.

[0017] Specifically, the diameter D is greater than or equal to 0.5 mm and less than or equal to 30 mm; the droplet volume is greater than or equal to 0.1 μL and less than or equal to 50 μL.

[0018] A biological experimental apparatus for the above-mentioned biological experimental method includes an experimental stage, two clamping mechanisms disposed on the experimental stage for clamping two reaction slides respectively, a driving module disposed on the experimental stage for driving the two clamping mechanisms to move relative to each other, and a light source module and an imaging module disposed on both sides of the two reaction slides respectively.

[0019] Specifically, the imaging module is positioned below the two reaction slides; the light source module is positioned above the two reaction slides; and the imaging module includes a liquid sensor and a bubble sensor.

[0020] Specifically, at least one of the clamping mechanisms may be optionally equipped with a temperature control module; the temperature control module is used to heat or cool the reaction slide.

[0021] Furthermore, the biological experimental device also includes a control system module, which is used to adjust the brightness and color of the light source module, acquire and analyze the state of the droplets and bubbles provided by the imaging module, and send commands to the drive module to perform lifting and lowering actions and speed control.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] 1. In biological experiments, this invention monitors the application process of reaction slides in real time. During the contact between the droplet and the upper and lower reaction slides, the invention dynamically raises and lowers the reaction slides and controls the speed in stages to directionally drive away or eliminate air bubbles in the target area, thereby improving experimental efficiency and the accuracy of results. At the same time, it reduces the difficulty of manual operation and the safety of samples, ensures the smooth progress of the experimental process, and obtains effective data results.

[0024] 2. This invention has low requirements for mechanical structure and control system. The reciprocating change of the spacing between the reaction slides utilizes three-dimensional unsteady fluid motion to remove bubbles, resulting in faster bubble removal and a higher success rate. At the same time, the relative motion between the two reaction slides greatly reduces the shear force of the droplets in the horizontal direction, which is more friendly to the samples attached to the reaction slides and will not cause sample displacement or damage.

[0025] 3. The present invention has a wider range of compatibility and can effectively remove different types of bubbles. It can be applied not only to the removal of tiny bubbles, but also to the removal of large bubble cavities that are in contact with two reaction slides at both ends. Attached Figure Description

[0026] Figure 1 This is a flowchart of the method described in this invention.

[0027] Figure 2 This is a schematic diagram of the droplet morphology during the attachment process of the method described in this invention.

[0028] Figure 3 This is a schematic diagram of two states of step S3 in the method described in this invention.

[0029] Figure 4 This is a schematic diagram of the droplet morphology in steps S4 and S5 of the method described in this invention.

[0030] Figure 5 This is one embodiment of the method and apparatus described in this invention.

[0031] Figure 6 This is another embodiment of the method and apparatus described in this invention.

[0032] Figure 7 This is another embodiment of the method and apparatus described in this invention.

[0033] Figure 8 These are two sets of images of the bubble removal process captured by the imaging module of this invention.

[0034] In the figure: 1. First reaction slide fixing module; 11. First reaction slide; 12. First clamping mechanism; 2. Second reaction slide fixing module; 21. Second reaction slide; 22. Droplet; 23. Bubble; 24. Target area; 25. Second clamping mechanism; 3. Drive module; 4. Light source module; 5. Imaging module; 6. Control system module. Detailed Implementation

[0035] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0036] In the description of this invention, it should be noted that directional terms such as "center," "lateral," "longitudinal," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise" indicate the orientation and positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. They should not be construed as limiting the specific protection scope of this invention.

[0037] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features. Thus, the use of "first" and "second" to define a feature may explicitly or implicitly include one or more of that feature, and in the description of this invention, "a number" means two or more, unless otherwise explicitly specified.

[0038] In this invention, unless otherwise explicitly specified and limited, terms such as "set" and "install" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can also refer to a mechanical connection; they can refer to a direct connection or a connection through an intermediate medium; or they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0039] See also Figure 1-4 As shown, a biological experimental method includes the following steps:

[0040] Step S1: Prepare two reaction slides, mount at least one of the reaction slides on the clamping mechanism, and drop a quantitative reaction solution (reagent or sample) onto the target area 24 on the second reaction slide 21 to form a droplet 22. The first reaction slide 11 is positioned at the initial spacing L0 on the upper part of the second reaction slide 21. In a specific embodiment, a 10 μL reaction droplet forms a circular droplet 22 of approximately φ4.5 mm on the second reaction slide 21, with an initial spacing L0 = 4 mm.

[0041] In step S2, the first reaction slide 11 is brought closer together at a first speed V1, and the distance between the two reaction slides is monitored in real time. When the distance reaches a preset critical distance L1, the movement is switched to a second speed V2 (V2 < V1). In a specific embodiment, the first speed V1 = 0.8 mm / s, the second speed V2 = 0.05 mm / s, and the preset critical distance L1 = 1 mm.

[0042] In step S3, droplet 22 is compressed between the two reaction slides to form a liquid film. An imaging module positioned below the second reaction slide 21 captures the liquid film image in real time, and an image recognition algorithm is used to determine whether bubbles exist in the target area.

[0043] If there are no bubbles in the target area (e.g.) Figure 3 If state 3b is shown, proceed directly to step S7; if bubbles 23 appear in the target area (e.g.) Figure 3 When in state 3a), record the distance between the first reaction slide 11 and the second reaction slide 21 as L2, and execute steps S4, S5, S6 and S7 in sequence;

[0044] Step S4 as follows Figure 4As shown, the first reaction slide 11 is controlled to move in the opposite direction at a third speed V3 (V3 = 0.1-10 mm / s, V3 > V2) to a preset critical distance L1, during which the liquid film is subjected to shear force and migrates towards the edge of the liquid film. In a specific embodiment, the first reaction slide 11 returns to a distance of 1 mm from the second reaction slide 21 at a third speed V3 = 1.5 mm / s.

[0045] After completing the reverse displacement in step S5, the forward bonding action is resumed, and the slide returns at the second speed V2 until the imaging module determines that the liquid film covers the target area 24. At this time, the distance between the two reaction slides is L3 (L min <L3≤L2). In a specific embodiment, L3 = 0.6 mm;

[0046] Step S6 repeats steps S4 and S5, with a maximum number of iterations n≤100 times (preferably 1≤n≤5 times), until the imaging module determines that the bubble 23 has been eliminated or migrated outside the target area 24.

[0047] After dynamic venting is completed in step S7, the two reaction slides continue to be bonded together until the minimum spacing L is reached. min The liquid film evenly covers the target area 24, completing the air bubble removal and adhesion. In a specific embodiment, based on the actual viscosity of the liquid and the different surface roughness of the glass slide, L... min It is 5-10 μm.

[0048] The first reaction slide 11 can be a glass slide, coverslip, slide with a thin layer of the tissue to be tested attached, or a pre-prepared bioreaction chip, etc. The second reaction slide 21 can be a glass slide, coverslip, slide with a thin layer of the tissue to be tested attached, or a pre-prepared bioreaction chip, etc. The first reaction slide 11 and the second reaction slide 21 need to be aligned during the experiment to ensure that the reaction areas at their corresponding positions coincide with or are within the target reaction area set in the experiment.

[0049] The droplets typically have a diameter of 0.5-30 mm, preferably 2-10 mm, corresponding to a droplet volume of approximately 0.1-50 μL. The initial spacing L0 is typically 0.01 mm to 100 mm, preferably 5 to 20 mm. The first velocity V1 is typically 0.1 to 100 mm / s, preferably 1 to 10 mm / s. The preset critical distance L1 < L0, preferably 0.1 to 3 mm. The spacing L3 ≤ L1, and L3 > L0. min Preferably, the spacing is 0.1 to 5 mm. The second speed V2 < V1, preferably set to 0.01 to 1 mm / s. The third speed V3 > V2, preferably set to 0.1 to 10 mm / s. The number of cycles n ≤ 100, preferably n = 1 to 5 times.

[0050] See Figure 5 A biological experimental device includes an experimental platform, two clamping mechanisms disposed on the experimental platform for clamping two reaction slides respectively, a drive module 3 disposed on the experimental platform for driving the relative movement of the two clamping mechanisms, a light source module 4 and an imaging module 5 disposed on both sides of the two clamping mechanisms respectively, and a control system module 6; the clamping mechanisms are the first reaction slide fixing module 1 and the second reaction slide fixing module 2.

[0051] The first reaction slide fixing module 1 includes a first reaction slide 11 and its first clamping mechanism 12; the second reaction slide fixing module 2 includes a second reaction slide 21 and its second clamping mechanism 25; biological samples or reagents can be dropped onto the target area 24 on the second reaction slide 21 to form droplets 22; the driving module 3 includes a guiding component (such as a linear guide rail or linear bearing), a motor and a speed transmission component; the first reaction slide fixing module 1 and the driving module 3 are coupled together, and the driving module 3 can drive the first reaction slide fixing module 1 to realize lifting and lowering actions and speed switching, and adjust the distance between the first reaction slide 11 and the second reaction slide 21.

[0052] The light source module 4 and the imaging module 5 are arranged on the upper and lower sides of the first reaction slide 11 and the second reaction slide 21. The light source module 4 and the imaging module 5 can capture or acquire in real time the image of the droplet 22 during the bonding process of the first reaction slide 11 and the second reaction slide 21. The control system module 6 integrates a control unit, which can adjust the light source intensity of the light source module 4, acquire and analyze the state of the droplets and bubbles during the experiment, and send commands to the drive module 3 to perform lifting and lowering actions, speed control, and optimize image quality.

[0053] In one embodiment, the observation process is completed by the experimental operator through visual perception system, and the operator determines whether the experimental requirements are met and whether to proceed with subsequent steps. In another embodiment, the observation process is completed by sensors (such as liquid sensors, bubble sensors, etc.) pre-set inside the instrument, and the results are fed back to the experimental operator or controller, who then determines whether the experimental process meets the requirements. In yet another embodiment, the observation process is detected in real time by a visual recognition device (such as a CCD, CMOS camera, etc.) and judged in conjunction with an image recognition algorithm.

[0054] like Figure 6In another embodiment shown, the second reaction slide fixing module 2 is coupled to the driving module 3. The driving module 3 can drive the second reaction slide fixing module 2 to realize lifting and lowering actions and speed switching, and adjust the distance between the first reaction slide 11 and the second reaction slide 21.

[0055] like Figure 7 In another embodiment shown, the driving module 3 of the present invention can also be manually operated to control the first reaction slide fixing module 1 to adjust the distance between the first reaction slide 11 and the second reaction slide 21. The experimenter completes this by visually observing through the imaging module 5, and then judges whether the experimental requirements are met, and subsequently decides whether to proceed with the next steps.

[0056] At least one of the first reaction slide 11 and the second reaction slide 21 has a target region 24 of a predetermined geometry, the target region 24 being configured to contain biological samples or reagents and defined as a target reaction area. Preferably, the target region 24 is square.

[0057] At least one of the first clamping mechanism 12 and the second clamping mechanism 25 may be equipped with a heating / cooling module to heat or cool the reaction slide to adapt to different experimental conditions (such as isothermal biological reaction of samples or low temperature treatment).

[0058] Figure 8 These are two sets of bubble removal processes captured by the imaging module of this invention. During the unfolding process, bubbles are generated in the liquid film formed on the reaction slides, and a bubble removal mechanism is implemented. As the distance between the two reaction slides increases, the area of ​​the liquid film captured by the imaging module shrinks. During this process, the bubbles within the liquid film disappear after migrating to the edges. Once the distance between the two reaction slides reaches a critical distance, the adhesion is restored, and the experimental procedure is completed.

[0059] The technical solution proposed in this invention uses multiple changes in the distance h between two glass slides to remove bubbles. Because the glass slides are vertically pulled, the gap h increases over time, and the flow is not only shear flow but also involves compression or stretching flow. The velocity distribution of the fluid is no longer linear but a two-dimensional unsteady motion. When the two plates are pulled apart or brought closer, the fluid needs to flow in or out from the edge, thus generating a pressure gradient and velocity field. The calculation of shear force may not be as simple as that of Couette flow and requires the use of Reynolds equations or lubrication approximation theory. According to lubrication theory, for the vertical motion of incompressible fluid between parallel plates, the resulting pressure gradient and velocity distribution may be related to the time derivative. The total shear force expression is F=a*μ*A*(dh / dt) / h^3; where a is a constant, the specific value of which depends on boundary conditions and other factors, μ is the liquid viscosity, A is the area of ​​the liquid film between the two glass slides, and h is the thickness of the liquid film. dh / dt is the rate of change of the liquid film thickness in the vertical direction, i.e., the speed at which the glass slides rise or fall in this case.

[0060] Because the method in this case is based on three-dimensional fluid motion, and the magnitude of the total shear force is inversely proportional to the cube of the liquid film thickness, when the distance between the two glass slides increases, the shear force of the fluid decreases rapidly, while the normal stress of the fluid increases rapidly. Therefore, during the ascent of the upper glass slide, the bubbles adhering to the lower glass slide first leave the lower surface and are lifted up with the fluid motion. Then, during this process, they are crushed under the strong external normal stress, or pushed towards the center of the liquid film.

[0061] i) If the bubble is crushed, the bubble disappears; this is the first case of this method.

[0062] ii) If the bubble is pushed toward the center, since the liquid film (or liquid column) after being stretched is a cone shape, that is, smaller at the top and larger at the bottom, during the process of the slide descending, due to the release of the normal phase pressure, the bubble will quickly flow to the outside and then be discharged outside the target observation area. This is the second case of this method.

[0063] The advantages of this invention compared to existing technologies or cited prior art are as follows: 1) The device is simple and reliable, and can be operated manually; 2) It has high bubble removal efficiency, utilizing the three-dimensional unsteady fluid motion during the vertical ascent or descent of the glass slide to remove bubbles, resulting in significant removal effect and high efficiency; 3) It is more sample-friendly, as the fluid shear force along the horizontal plane of the glass slide is greatly reduced, making it more suitable for samples attached to the glass slide; 4) It can better handle various bubble types. When faced with large bubbles, the upper surface of the bubble will first separate from the upper glass slide, and then separate from the lower glass slide under the action of inward and upward fluid motion, and finally be crushed or flow out of the target observation area during the movement.

[0064] The above description is only a specific embodiment of the present invention, but the technical features of the present invention are not limited thereto. Any changes or modifications made by those skilled in the art within the scope of the present invention are covered by the patent scope of the present invention.

Claims

1. A biological experimental method, characterized in that: The steps include the following: S1. Prepare two reaction slides, install at least one of the reaction slides on the clamping mechanism, add a reaction liquid droplet to the target area of ​​at least one of the reaction slides to form a droplet with a diameter of D, and maintain an initial distance L0 between the two reaction slides. S2 causes the two reaction slides to approach each other at a first speed V1. When the distance between the two reaction slides reaches a preset critical distance L1, the speed is switched to a second speed V2 to continue approaching each other. The second speed V2 is less than the first speed V1. During the process of the two reaction slides approaching each other at the second speed V2 in S3, the droplet is gradually compressed and expands between the two reaction slides to form a liquid film. The liquid film is observed in real time to see if there are bubbles in the target area: if there are no bubbles in the target area, step S7 is executed directly; if there are bubbles in the target area, the distance between the two reaction slides is recorded as L2, and steps S4, S5, S6, and S7 are executed in sequence. S4 controls at least one of the reaction slides to move the two reaction slides away from each other at a third speed V3 until the distance between the two reaction slides reaches the preset critical distance L1, wherein the third speed V3 is greater than the second speed V2; S5 controls at least one of the reaction slides to move the two reaction slides closer together at the second speed V2 until the liquid film completely covers the target area. At this time, the distance between the two reaction slides is L3, where L... min <L3≤L2, L min This is the minimum distance that the two reaction slides need to achieve; S6 repeats steps S4 and S5 until no more bubbles are present in the target area; S7 continues to press and move closer together, the liquid film spreading evenly between the two reaction slides until the two reaction slides reach their minimum distance L. min The two reaction slides stop moving, completing the attachment process.

2. The biological experimental method as described in claim 1, characterized in that: The first velocity V1 is greater than or equal to 0.1 mm / s and less than or equal to 100 mm / s; the second velocity V2 is greater than or equal to 0.01 mm / s and less than or equal to 1 mm / s; and the third velocity V3 is greater than or equal to 0.1 mm / s and less than or equal to 10 mm / s.

3. The biological experimental method as described in claim 1, characterized in that: The initial spacing L0 is greater than or equal to 0.01 mm and less than or equal to 100 mm; the preset critical distance L1 is greater than or equal to 0.1 mm and less than or equal to 3 mm; and L3 is greater than or equal to 0.1 mm and less than or equal to 5 mm.

4. The biological experimental method as described in claim 1, characterized in that: The diameter D is greater than or equal to 0.5 mm and less than or equal to 30 mm; the droplet volume is greater than or equal to 0.1 μL and less than or equal to 50 μL.

5. A biological experimental apparatus, employing the biological experimental method according to any one of claims 1-4, characterized in that: It includes an experimental platform, two clamping mechanisms mounted on the experimental platform for clamping the two reaction slides respectively, a drive module mounted on the experimental platform for driving the relative movement of the two clamping mechanisms, and a light source module and an imaging module respectively mounted on both sides of the two reaction slides.

6. The biological experimental apparatus as described in claim 5, characterized in that: The imaging module is positioned below the two experimental slides; the light source module is positioned above the two reaction slides; the imaging module includes a liquid sensor and a bubble sensor.

7. The biological experimental apparatus as described in claim 5, characterized in that: At least one of the clamping mechanisms may be optionally equipped with a temperature control module; the temperature control module is used to heat or cool the reaction slide.

8. The biological experimental apparatus as described in claim 5, characterized in that: It includes a control system module, which is used to adjust the brightness and color of the light source module, acquire and analyze the state of the droplets and bubbles provided by the imaging module, and send commands to the drive module to perform lifting and lowering actions and speed control.

Images