Screening method of taxis compound of neopterus albus and luring or repelling application of neopterus albus

Abstract

The invention relates to a method for screening a chemotactic compound of neopterus albus and attraction or avoidance application of the neopterus albus. According to the method, differential compounds of volatile compounds in leaves of hobby host plants and non-hobby host plants of congeneric sibling species of neopterus albus under the same habitat are compared; a positive taxis compound and a negative taxis compound can be screened at the same time by selecting, from among these differential compounds, a compound having a particularly high content or characteristic content in a hobby host plant and / or a non-hobby host plant to carry out an indoor and outdoor insect trapping test. The method has the advantages of strong pertinence and high success rate, and greatly shortens the time for screening the taxis compound. In addition, the positive taxis compound screened from the specific volatile compounds can be used as an attractant for the neopterus albus, and the negative taxis compound can be used as a repellent for the neopterus albus.

Description

Technical Field

[0001] This invention relates to a method for screening insect tropism compounds, specifically a rapid screening method for positive and negative tropism compounds in the gall-causing aphid, *Neopteryx hygrophorus*, belonging to the field of entomology. Background Technology

[0002] *Distylium sieb. et Zucc.*, belonging to the Hamamelidaceae family of the Rosales order, is an important landscaping plant. From March to May each year, *Distylium spp.* plants in Hunan, Hubei, Jiangsu, and Anhui provinces frequently develop purplish-red, green, or yellowish-green galls on their leaves, leading to localized necrosis and drying. This is particularly severe in *Distylium racemosum* Sieb. & Zucc. (e.g.) Figure 1 As shown), *Distyliu yricoides* and *Distyliu chinense* cause relatively minor damage, while *Distyliu buxifolium* is almost unaffected (e.g.). Figure 2 (As shown). *Neothoracaphis yanonis Matsumura* and *Neothoracaphis hangzhouensis Zhang* are two major pests causing galls in *D. racemosum*. These two aphids are very similar in morphology and habits.

[0003] Insects choose specific plants as hosts not usually for nutritional reasons, but because different plant species possess unique secondary metabolites, and different insect species have specialized chemoreceptors. These secondary metabolites attract or repel certain insects. The nutrient content of the host plant plays a crucial role in the feeding selection of herbivorous insects, while plant secondary metabolites, especially volatile secondary metabolites, play a key role in the host selection for insect oviposition. Volatile compounds released by plants play an important communication and guidance role in the identification of host plants by herbivorous insects. Plants can attract insects to remotely locate and approach the host by releasing volatile signaling compounds, stimulating insects to land, feed, mate, mate, and lay eggs on the host.

[0004] Herbivorous insects often exhibit specificity in recognizing volatile signaling compounds. In-depth research on plant volatile signaling compounds not only helps to reveal the host selection mechanisms of herbivorous insects, but also provides a theoretical basis for the development of attractants and repellents for volatile plant-derived pests, and even new pest control strategies.

[0005] Aphids, including the mosquito aphid (Neopteryx fasciatus), have multiple generations per year, with winged and wingless aphids alternating in generation. Aphids mainly migrate and spread through winged aphids, making the trapping and control of winged aphids crucial to preventing their spread. Developing attractants for winged aphids is an important aphid control measure, while developing repellents for winged aphids can prevent or reduce aphid damage to relevant host plants.

[0006] Currently, research on positive tropism compounds or attractants and negative tropism compounds or repellents for pests often needs to be conducted independently rather than simultaneously. Furthermore, their screening process is somewhat blind, time-consuming, labor-intensive, and has a low success rate. Summary of the Invention

[0007] In view of the above problems, according to one objective of the present invention, a method for rapidly screening insect tactic compounds (positive and negative tactic compounds) is provided. Specifically, this method provides a method for simultaneously screening positive and negative tactic compounds of *Neothoracaphisyanonis* by rapidly screening insect tactic compounds of closely related host species with significantly different damage conditions.

[0008] According to one aspect of the present invention, a method for screening tactic compounds for the mosquito larvae *Neopteryx* is provided, wherein the tactic compounds include positive and negative tactic compounds, characterized by comprising the steps of:

[0009] The preferred and non-preferred host plants of the mosquito aphid were observed and screened under the same time period and habitat.

[0010] Fresh leaves were obtained from the selected preferred host plants and non-preferred host plants respectively, and a first volatile component was collected from the fresh leaves of the preferred host plants, and a second volatile component was collected from the fresh leaves of the non-preferred host plants.

[0011] Compare and analyze the compounds that differ between the first volatile component and the second volatile component;

[0012] Compounds present in the first volatile component but not in the second volatile component were selected as suspected positive tropism compounds of the mosquito mother aphid, and / or

[0013] Compounds present in the second volatile component but not in the first volatile component were selected as suspected negative tropism compounds of the mosquito mother aphid.

[0014] Insect attraction experiments were conducted using the suspected positive tropism compound and the suspected negative tropism compound, respectively, to screen out the positive and negative tropism compounds of the mosquito mother aphid.

[0015] According to one embodiment, the preferred host plant and the non-preferred host plant are closely related species of the same genus. Preferably, the preferred host plant and the non-preferred host plant are both from the genus *Acer buergerianum*; the preferred host plant is *Acer buergerianum*, and the non-preferred host plant is *Acer buergerianum* var. *microphyllum*.

[0016] According to another embodiment, the first volatile component and the second volatile component are collected by solid-phase microextraction; the differential compounds are detected, compared and analyzed by gas chromatography-mass spectrometry.

[0017] According to another embodiment, insect attraction tests were conducted using the suspected positive and negative tactic compounds to screen for positive and negative tactic compounds of *Neopteryx hygrophorus*. This included observing the attraction of *Neopteryx hygrophorus* winged aphids to the suspected positive and negative tactic compounds indoors using a four-arm olfactory instrument, and observing the insect attraction effect of the suspected positive and negative tactic compounds on *Neopteryx hygrophorus* winged aphids outdoors using sticky insect boards or traps. Suspected positive tactic compounds that attracted more insects than the blank control were identified as positive tactic compounds for *Neopteryx hygrophorus*, and suspected negative tactic compounds that attracted fewer insects than the blank control were identified as negative tactic compounds for *Neopteryx hygrophorus*.

[0018] According to yet another embodiment, the positive chemotactic compound is 1-octen-3-ol, and the negative chemotactic compound is one or both of acetic acid leaf ester and cis-3-hexenyl butyrate.

[0019] According to another aspect of the invention, an attractant for the mosquito larvae Neothorax is provided, comprising 1-octen-3-ol.

[0020] According to another aspect of the present invention, a repellent for the mosquito larvae Neothorax is provided, comprising one or both of ethyl acetate and cis-3-hexenyl butyrate.

[0021] According to another aspect of the invention, 1-octen-3-ol is provided for use in attracting the mosquito mother aphid.

[0022] According to another aspect of the invention, acetic acid leaf ester and / or butyrate cis-3-hexenyl ester are provided for use in repelling the mosquito mother aphid.

[0023] This method is highly targeted and can quickly and simultaneously screen for both positive and negative tropism compounds of the mosquito mother aphid from two different plants, greatly improving work efficiency. Attached Figure Description

[0024] Figure 1 The image shows a tree infested with the mosquito mother aphid;

[0025] Figure 2This shows a small-leaved tussock tree that is not harmed by the tussock aphid;

[0026] Figure 3 The GC-MS spectra of a young leaf sample of *Cymbidium goeringii* according to Example 1 are shown.

[0027] Figure 4 The GC-MS spectra of young leaf samples from *Mammillaria pulcherrima* according to Example 1 are shown.

[0028] Figure 5 The GC-MS spectra of young leaf samples from *Cymbidium goeringii* according to Example 2 are shown.

[0029] Figure 6 The GC-MS spectrum of a young leaf sample of *Symplocos simonii* according to Example 2 is shown. Detailed Implementation

[0030] The method for rapidly screening insect tropism compounds (simultaneously screening positive and negative tropism compounds) according to the present invention is applicable to the rapid screening of tropism compounds in insects such as Neothoracaphis yanonis, which exhibit significant differences in damage to closely related host species. The screened positive tropism compounds can be used to prepare attractants, and the negative tropism compounds can be used to prepare repellents.

[0031] According to one specific embodiment, the method of the present invention for screening tactic compounds of the mosquito mother aphid, the tactic compounds including positive tactic compounds and negative tactic compounds, includes the following steps.

[0032] Step 1: Observe and screen the preferred and non-preferred host plants of the mosquito aphid under the same time period and habitat. Preferred host plants are generally defined as those with a fresh leaf infestation rate greater than 30%, while non-preferred host plants are generally defined as those with a fresh leaf infestation rate less than 1%. Here, "fresh leaves" refers to fresh, young leaves. In one embodiment, preferred and non-preferred host plants are closely related species within the same genus. Closely related species have similar chemical compositions (including volatile secondary metabolites), which narrows the screening range when comparing the differences in volatile compounds within preferred and non-preferred host plants, thereby improving screening efficiency. Furthermore, selecting preferred and non-preferred host plants from the same habitat ensures consistent growth conditions, avoiding changes in host plant compounds caused by inconsistent growth conditions, which could increase uncertainty in the screening process.

[0033] According to one embodiment, the preferred and non-preferred host plants can be of the genus *Distylium* spp. For example, the preferred host plant can be *Distylium racemosum* Sieb. & Zucc., and the non-preferred host plant can be *Distylium buxifolium*.

[0034] Step 2: Fresh leaves are obtained from the selected preferred and non-preferred host plants, respectively. A first volatile component is collected from the fresh leaves of the preferred host plants, and a second volatile component is collected from the fresh leaves of the non-preferred host plants. The fresh leaves are preferably fresh, young leaves. According to one embodiment, the first and second volatile components are collected by solid-phase microextraction.

[0035] Step 3: Compare and analyze the differences between the first volatile component and the second volatile component.

[0036] Step 4: Select compounds contained in the first volatile component but not in the second volatile component as suspected positive tactile compounds of *Neopteryx fasciatus*, and / or select compounds contained in the second volatile component but not in the first volatile component as suspected negative tactile compounds of *Neopteryx fasciatus*.

[0037] According to one embodiment, the aforementioned differential compounds can be detected, compared, and analyzed using gas chromatography-mass spectrometry (GC-MS).

[0038] Step 5: Use the above-mentioned suspected positive tropism compounds and the above-mentioned suspected negative tropism compounds to conduct insect attraction tests to screen out the positive tropism compounds and negative tropism compounds of the mosquito mother aphid.

[0039] According to one embodiment, the step may include:

[0040] Indoors, a four-armed olfactory instrument was used to observe the tropism of the winged aphid *Neopterygii* to the aforementioned suspected positive and negative tropism compounds.

[0041] Outdoors, sticky traps or baits were used to observe the insect-attracting effects of suspected positive and negative tropism compounds on the winged aphid *Neopteryx hygrophorus*.

[0042] The suspected positive tactic compounds that attract more insects than the blank control are positive tactic compounds for *Neopteryx hymenatus*, for example, the number of insects attracted by the positive tactic compound is 2 to 3 times that of the blank control. The suspected negative tactic compounds that attract fewer insects than the blank control are negative tactic compounds for *Neopteryx hymenatus*, for example, the number of insects attracted by the negative tactic compound is significantly less than that of the blank control, for example, the damage rate on fresh leaves can be kept below 1%.

[0043] According to one embodiment, for both preferred and unpreferred host plants of the genus *Neopteryx*, the positive tropism compound for *Neopteryx nigra* is 1-octen-3-ol, and the negative tropism compound is cis-3-hexenyl butyrate.

[0044] According to one specific embodiment, the method for screening tropist compounds of the mosquito larvae *Neopteryx fasciatus* according to the present invention includes the following steps:

[0045] (1) Determination of preferred and non-preferred hosts and analysis of volatile compounds in leaves of the closely related species *Neopteryx hygrophorus*.

[0046] To identify the preferred and non-preferred host plants of closely related species of *Neopteryx hymenatus* in the same habitat and at the same time period, namely *Neopteryx hymenatus* and *Neopteryx microphylla*, fresh young leaves were collected from the preferred host plant (*Neopteryx hymenatus*) and the non-preferred host plant (*Neopteryx microphylla*) of *Neopteryx hymenatus* in the same habitat and at the same time period.

[0047] Volatile components of two fresh young leaves were collected by solid-phase microextraction, and the differences in compounds were compared and analyzed by GC-MS.

[0048] (2) Select specific volatile compounds suspected of having attractant or repellent effects from compounds with different volatility.

[0049] From the volatile differential compounds of the genus *Neopteryx* (*Neopteryx spp.*) of the preferred host plant (*Neopteryx spp.*) and the non-preferred host plant (*Neopteryx spp.*), one or more compounds with particularly high content in the preferred host plant and / or non-preferred host plant (e.g., selecting the top one to three components in terms of content) or unique to the non-preferred host plant are selected as suspected tactic compounds for indoor and outdoor insect attraction experiments.

[0050] (3) Indoor and outdoor insect attraction tests using suspected tactic compounds

[0051] Indoors, a four-armed olfactory instrument was used to observe the tropism of different compounds on the winged aphid *Neopterygii*. Outdoors, sticky traps or baits were used to observe the insect-attracting effect of different suspected tropism compounds on the winged aphid *Neopterygii*. If the number of insects attracted was significantly higher than that of the blank control (e.g., 2-3 times that of the blank control), it indicates positive tropism on *Neopterygii* and can be used as a positive tropism compound (i.e., attractant compound). If the number of insects attracted was significantly lower than that of the blank control (e.g., 1 / 4 to 1 / 8 that of the blank control), it indicates negative tropism on *Neopterygii* and can be used as a negative tropism compound (i.e., repellent compound).

[0052] According to another embodiment of the present invention, an attractant for the mosquito larvae *Neopteryx fasciatus* is provided, comprising the above-screened positive tactic compound. Further, the positive tactic compound may be 1-octen-3-ol.

[0053] According to another embodiment of the present invention, a repellent for the mosquito larvae *Neopteryx* is provided, comprising the aforementioned screened negative repellent compound. Further, the negative repellent compound may be one or both of ethanol acetate and cis-3-hexenyl butyrate. According to one embodiment, the combined use of ethanol acetate and cis-3-hexenyl butyrate yields a better repellent effect.

[0054] According to another embodiment of the present invention, the application of the above-mentioned tactic compounds in the monitoring and control of the winged aphid *Neopteryx fasciatus* is provided. Specifically, 1-octen-3-ol is used to attract *Neopteryx fasciatus*. Leaf ester acetate and / or cis-3-hexenyl butyrate are used to repel *Neopteryx fasciatus*.

[0055] The present invention will be further described in detail below with reference to specific embodiments. These embodiments are only used to illustrate the present invention and do not limit the scope of the present invention.

[0056] Example 1

[0057] In March 2021, in the green belt of Xueyuan Road, Louxing District, Loudi City, Hunan Province, we observed and selected *Aphidius chinensis* plants with a damage rate of more than 30% (i.e., the preferred host of *Aphidius chinensis*) on fresh tender leaves and *Aphidius simonii* plants with a damage rate of less than 1% (i.e., the non-preferred host of *Aphidius chinensis*).

[0058] Fresh young leaves of *Ligustrum lucidum* and *Ligustrum sinense* were collected. Volatile components of both species were collected using solid-phase microextraction (65 μm PDMS / DVB solid-phase microextraction head). GC-MS (DB-5MS capillary column; mass spectrometry conditions: electron energy 70 eV, interface temperature 280℃, detector voltage 350 V, electron impact ionization (EI) source, ion source temperature 150℃, scan mass range 35–335 amu) was used for separation and detection. Identification of the volatiles was performed using a computer spectral library (NIST05) combined with literature review. Quantitative analysis was performed using peak area normalization to calculate the relative content of each component. The results are shown in [Figures to be inserted here]. Figure 3 and Figure 4 middle.

[0059] Depend on Figure 3 and Figure 4 The results showed that the content of volatile compounds in the leaves of the preferred host plant of *Neopteryx* (*Neopteryx spp.*) was significantly higher than that in the leaves of the non-preferred host plant (*Neopteryx microphylla*), namely cis-3-hexen-1-ol (42.72%). Figure 3 RT5.73), 3-octanone (11.34%) Figure 3, RT11.34), etc., and 1-octen-3-ol (7.41%), which is specific to the preferred host plant (Mosla chinensis) but not detected in the preferred host plant (Mosla chinensis var. spp.) (and not detected in the preferred host plant (Mosla chinensis var. spp.)). Figure 3 RT11.12), and the highest content of acetic acid leaf ester (46.07%) in non-addictive host plants (small-leaved privet tree) (RT11.12). Figure 4 RT12.36 was also used as a comparison compound. In March 2024, the standards (chromatographic grade) of the above four volatile compounds were purchased from Shanghai Anpu Cuishi Standard Technology Service Co., Ltd. and Anpu Cloud Laboratory Supplies (Shanghai) Co., Ltd. for the next step of the experiment.

[0060] Indoor insect attraction experiment: From 19:00 to 21:00 at night, a four-armed olfactometer was placed directly below an indoor light source. 0.5 ml of each of the four volatile compound standards was placed in one of the four sample vials of the olfactometer. Thirty adult *Neopterygii hymenatus* winged aphids were introduced into the center of the olfactometer's active chamber. A silent, oil-free vacuum pump was turned on (average flow rate 10 L / min, relative vacuum -88 kPa). After 0.5 hours, the tropism of the 30 *Neopterygii hymenatus* winged aphids towards different compounds was observed in the olfactometer. It was found that the number of aphids in the 1-octen-3-ol sample vial was significantly higher than the number in the sample vials containing other volatile compounds. Specific results are shown in Table 1.

[0061] Table 1 Indoor tropism test of *Neopteryx fasciatus* (Mosquito mother aphid)

[0062]

[0063] Note: Data in the same column with the same letter after it are not significantly different (p<0.05).

[0064] Outdoor insect-attracting experiment: 0.5 ml of each of the four volatile compound standards was applied to cotton balls. The cotton balls with the added volatile compounds were then fixed to sticky insect boards (25cm × 20cm, yellow) and hung approximately 1.5m high in the green belt of the *Nematophora muscaria* tree. Sticky insect boards without added volatile compounds were used as a control. The insect-attracting effects of different specific volatile compounds on the winged aphid *Nematophora muscaria* were observed after 24 hours. The results are shown in Table 2.

[0065] Table 2 shows that sticky insect traps containing cotton with added 1-octen-3-ol attracted significantly more winged aphids than those containing cotton with other compounds. 3-Octanone was the next most effective, while leaf ester acetate attracted significantly fewer aphids than the control group. This indicates that 1-octen-3-ol, unique to the leaves of the preferred host plant (*Gnaphalium affine*), has a significant attractant effect on winged aphids, while leaf ester acetate, the most abundant compound in the leaves of the non-preferred host plant (*Gnaphalium affine*), has a repellent effect on winged aphids.

[0066] Table 2 Outdoor trapping test of *Neopterygii* (a type of mosquito)

[0067]

[0068]

[0069] Note: Data in the same column with the same letter after it are not significantly different (p<0.05).

[0070] Example 2

[0071] In August 2020, in the green belt of Xueyuan Road, Louxing District, Loudi City, Hunan Province, we observed and selected *Neopteryx chinensis* plants with a damage rate of more than 30% (i.e., the preferred host of *Neopteryx chinensis*) on fresh tender leaves and *Neopteryx microphylla* plants with a damage rate of less than 1% (i.e., the non-preferred host of *Neopteryx chinensis*).

[0072] Following the same method as in Example 1, the volatile components of both compounds were collected by solid-phase microextraction, and the differences were compared and analyzed using GC-MS. The results are shown in [Figures 1-2]. Figure 5 and Figure 6 middle.

[0073] Depend on Figure 5 and Figure 6 The results showed that the content of volatile compounds in the leaves of the preferred host plant of *Neopteryx* (*Neopteryx spp.*) was significantly higher than that in the leaves of the non-preferred host plant (*Neopteryx microphylla*), namely cis-3-hexen-1-ol (39.95%). Figure 5 RT6.91), 1,3,5,7-cyclooctatetraene (12.38%) Figure 5 RT7.52), etc., and 1-octen-3-ol (1.55%), which is specific to the preferred host plant (Mosla chinensis) but not detected in the preferred host plant (Mosla chinensis var. spp.) (and not detected in the preferred host plant (Mosla chinensis var. spp.)). Figure 5 RT13.97) and cis-3-hexenyl butyrate (5.03%), which is present in higher amounts in non-loving hosts (small-leaved mosquito mother trees). Figure 6 RT28.32 was also used as a comparison compound. Figure 6 In February 2025, the above four volatile compounds were purchased as standards (analytical grade) from Shanghai Anpu Cuishi Standard Technical Service Co., Ltd. and Anpu Cloud Experimental Supplies (Shanghai) Co., Ltd. for further testing.

[0074] Indoor insect trapping experiment: From 19:00 to 21:00 at night, a four-armed olfactometer was placed directly below an indoor light source. 0.5 ml of each of the four volatile compound standards was placed in one of the four sample vials of the olfactometer. Thirty adult *Neopterygii* winged aphids were introduced into the center of the olfactometer's active chamber. A silent, oil-free vacuum pump was turned on (average flow rate 10 L / min, relative vacuum -88 kPa). After 0.5 hours, the tropism of the 30 *Neopterygii* winged aphids for different compounds was observed in the olfactometer. It was found that the number of aphids selecting 1-octen-3-ol was significantly higher than the number of aphids in the sample vials containing other volatile compounds. Specific results are shown in Table 3.

[0075] Table 3 Indoor insect attraction test for *Neopteryx fasciatus*.

[0076]

[0077] Note: Data in the same column with the same letter after it are not significantly different (p<0.05).

[0078] Outdoor insect-attracting experiment (compound dosage doubled): The dosage of the four volatile compound standards was doubled (1 ml) and applied to cotton. The cotton with the volatile compounds was fixed to a sticky insect board (25cm × 20cm, yellow) and hung at a height of about 1.5m in the green belt of the mosquito tree. Sticky insect boards without cotton containing volatile compounds were used as a control. The insect-attracting effect of different specific volatile compounds on the winged aphid *Neopteryx hymenatus* was observed after 24 hours. The results are shown in Table 4.

[0079] Table 4 shows that after doubling the dosage, sticky insect boards with cotton containing 1-octen-3-ol attracted more than 50 winged aphids of *Neopteryx chinensis*, while sticky insect boards with cotton containing other compounds and the control did not attract any *Neopteryx chinensis*. This indicates that 1-octen-3-ol, unique to the leaves of the preferred host plant (*Neopteryx chinensis*), has a significant attractant effect on winged aphids of *Neopteryx chinensis*, while cis-3-hexenyl butyrate, which is present in higher amounts in the leaves of the non-preferred host plant (*Neopteryx microphylla*), has a repellent effect on winged aphids of *Neopteryx chinensis*.

[0080] Table 4 Outdoor trapping test of *Neopterygii* (a type of mosquito)

[0081]

[0082]

[0083] Note: Data in the same column with the same letter after it are not significantly different (p<0.05).

[0084] In summary, the method of this invention can significantly shorten the time required to screen for tropism compounds in the winged aphid *Neopteryx hygrophorus*, increasing the success rate. Furthermore, the strongly tropist compound (1-octen-3-ol) screened from the specific volatile compounds can be used as an attractant for *Neopteryx hygrophorus* and can be used for monitoring and control of the winged aphid using insect traps or other insect-attracting devices; while the negatively tropist compounds (acetic acid leaf alcohol ester, cis-3-hexenyl butyrate) can be used as repellents for *Neopteryx hygrophorus*, serving as protectants for the host plants of *Neopteryx hygrophorus*.

Claims

1. A method for screening tactic compounds in the mosquito mother aphid, wherein the tactic compounds include positive and negative tactic compounds, characterized in that, Including the following steps: The preferred and non-preferred host plants of the mosquito aphid were observed and screened under the same time period and habitat. Fresh leaves were obtained from the selected preferred host plants and non-preferred host plants respectively, and a first volatile component was collected from the fresh leaves of the preferred host plants, and a second volatile component was collected from the fresh leaves of the non-preferred host plants. Compare and analyze the compounds that differ between the first volatile component and the second volatile component; Compounds present in the first volatile component but not in the second volatile component were selected as suspected positive tropism compounds of the mosquito mother aphid, and / or Compounds present in the second volatile component but not in the first volatile component were selected as suspected negative tropism compounds of the mosquito mother aphid. Insect attraction experiments were conducted using the suspected positive tropism compound and the suspected negative tropism compound, respectively, to screen out the positive and negative tropism compounds of the mosquito mother aphid.

2. The method according to claim 1, wherein, The preferred host plants and the non-preferred host plants are closely related species of the same genus.

3. The method according to claim 2, wherein, The preferred host plants and the non-preferred host plants are both from the genus *Mammillaria*. The preferred host plant is *Ligustrum lucidum*, and the non-preferred host plant is *Ligustrum microphyllum*.

4. The method according to claim 1, wherein, The first volatile component and the second volatile component were collected by solid-phase microextraction. The differential compounds were detected, compared, and analyzed using gas chromatography-mass spectrometry.

5. The method according to claim 1, wherein, Insect attraction experiments were conducted using the suspected positive and negative tropism compounds to screen for the mosquito mother aphid, including: The tropism of the winged mosquito mother, *Neopterygii*, to the suspected positive tropism compound and the suspected negative tropism compound was observed indoors using a four-arm olfactory instrument. Outdoors, sticky traps or baits were used to observe the insect-attracting effects of the suspected positive tropism compound and the suspected negative tropism compound on the winged aphid *Neopteryx hygrophorus*. The suspected positive tactic compounds that attracted more insects than the blank control were positive tactic compounds of *Neopteryx hygrophorus*, and the suspected negative tactic compounds that attracted fewer insects than the blank control were negative tactic compounds of *Neopteryx hygrophorus*.

6. The method according to claim 1, wherein, The positive chemotactic compound is 1-octen-3-ol, and the negative chemotactic compound is one or both of acetic acid leaf ester and cis-3-hexenyl butyrate.

7. An attractant for the mosquito nymph Neothorax, comprising 1-octen-3-ol.

8. A repellent for the mosquito larvae Neothorax, comprising one or both of acetic acid leaf ester and cis-3-hexenyl butyrate. 9,1-Octen-3-ol was used to attract the female mosquito, *Neopteryx fasciatus*.

10. Application of leaf ester acetate and / or cis-3-hexenyl butyrate for repelling the mosquito mother aphid.

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