An automated sterile sampling and cryopreservation integrated device

Abstract

The application discloses an automatic sterile sampling and low-temperature preservation integrated equipment, and belongs to the technical field of microbial fermentation, comprising a storage mechanism for storing and taking reagents, a blockage cleaning mechanism arranged in the storage mechanism for cleaning pipelines to avoid blockage, and a sending-out mechanism for sending out the reagents; the storage mechanism is provided with precise positioning, closed conveying and ultraviolet disinfection system, realizes automatic sampling, storage and taking of the reagents, does not need manual intervention in the whole process, the equipment is always isolated from the outside world when taking, microbial contamination is effectively avoided, and the accuracy of experimental data and the safety of the reagents are ensured; the application adopts a rotary cleaning cylinder, cooperates with a high-pressure water flow to drive a scraper to rotate and clean the inner wall of the conveying pipeline, completely removes residual reagents, prevents pipeline blockage and cross contamination, and significantly improves the long-term stability and reliability of the equipment.

Description

Technical Field

[0001] This invention relates to the field of microbial fermentation technology, and in particular to an automated aseptic sampling and low-temperature preservation integrated device. Background Technology

[0002] In the field of microbial fermentation technology, reagent sampling, preservation, and sterilization are crucial for ensuring the safety and efficiency of experimental and production processes. Traditional methods for handling microbial reagents typically rely on manual operation, which has the following technical drawbacks: Traditional methods often use chemical disinfectants or simple ultraviolet irradiation, making it difficult to achieve complete sterilization of pipe and container inner walls, easily leading to microbial contamination and affecting reagent purity and experimental results; conventional refrigeration equipment cannot precisely control reagent storage temperature, and frequent opening and closing of the door can cause temperature fluctuations, increasing the risk of reagent deterioration; sampling, sample retention, and cleaning processes rely on manual intervention, which is cumbersome and prone to introducing contamination, especially in continuous fermentation processes where aseptic and standardized operations are difficult to achieve; reagents (such as high-viscosity fermentation broth) easily leave scale residues in the transport pipelines, which are difficult to clean manually, and long-term accumulation may cause cross-contamination or system malfunctions. While some automated sampling equipment exists, its complex structure (e.g., relying on multi-axis sliding rail positioning) results in high maintenance costs and a lack of integrated sterilization and low-temperature preservation functions. Furthermore, traditional cleaning methods (such as high-pressure water flushing) are insufficient to thoroughly remove residues from the inner walls of pipes, affecting the purity of subsequent reagents. Therefore, there is an urgent need for a device that integrates automatic disinfection, precise sample retention, low-temperature preservation, and pipeline self-cleaning functions to solve the aforementioned technical bottlenecks in the field of microbial fermentation. Summary of the Invention

[0003] To address the aforementioned technical problems, the present invention adopts the following technical solution: an automated aseptic sampling and low-temperature preservation integrated device, comprising a storage mechanism for storing and retrieving reagents, the storage mechanism comprising a housing, and the storage mechanism being provided with a cleaning mechanism for cleaning pipes to avoid blockage and a delivery mechanism for delivering reagents, the cleaning mechanism comprising an inlet pipe fixedly installed on the housing;

[0004] The storage mechanism includes a placement plate fixedly installed inside the box, a storage box fixedly installed on the placement plate, a plurality of test tubes for storing reagents being provided inside the storage box, and a cooling pipe being provided inside the storage box.

[0005] Furthermore, the storage mechanism also includes four wheels at the bottom of the box, an ultraviolet disinfection tube inside the box, an intake fan and an exhaust fan on the box, a compressor fixedly installed inside the box, a door rotatably installed on the box, a cooling pipe fixedly installed on the compressor, and the other end of the cooling pipe connected to a cooling pipe inside the storage box.

[0006] Furthermore, a fixed base is fixedly installed on the placement plate, a rotating column is rotatably installed inside the fixed base, a swing arm motor is fixedly installed on the fixed base, the swing arm motor drives the rotating column to rotate through the swing arm belt, a rotating arm motor is fixedly installed on the rotating column, a motor gear is fixedly installed on the motor shaft of the rotating arm motor, a rotating arm is rotatably installed on the rotating column, a rotating arm gear is fixedly installed on the rotating arm, the rotating arm gear meshes with the motor gear, a front arm is rotatably installed on the rotating arm, a column is fixedly installed on the front arm, and a rotating arm transmission belt is wound around the rotating column and the column.

[0007] Furthermore, a lifting motor is fixedly installed on the column, a vertical lead screw is rotatably installed inside the front arm, and a lifting seat is slidably installed inside the front arm. The lifting seat and the vertical lead screw form a threaded transmission. A sampling tube is fixedly installed below the lifting seat. The lifting seat is connected to the inlet tube. The lifting motor drives the vertical lead screw to rotate through a lifting belt.

[0008] Furthermore, a waste container is fixedly installed on the box body, and a hole with a diameter larger than the outer diameter of the sampling tube is provided on the top of the waste container. A sewage pipe is fixedly installed below the waste container and is connected to an external sewage pipe.

[0009] The reagents to be stored are pumped through the inlet tube to the lifting seat, and finally enter the test tubes in the storage box through the sampling tube. The rotating arm motor drives the motor gear to rotate, which in turn drives the rotating arm gear and the rotating arm motor to rotate. As the rotating arm rotates, the front arm moves together. When the rotating column is not rotating, the rotating arm transmission belt drives the column and the front arm to rotate, so that the sampling tube always moves along the horizontal line connecting the sampling tube axis and the rotating column axis. The swing arm motor drives the rotating column to rotate relative to the fixed seat through the swing arm belt. Through the combination of the two actions, the sampling tube can reach the top of all the test tubes in the storage box, and the top of the waste tank and the discharge test tube. The rotating arm transmission belt and the front arm replace the complex slide rail structure, making the equipment lighter. The lifting motor drives the vertical screw to rotate through the lifting belt, which drives the lifting seat and the sampling tube to rise and fall, so that the sampling tube can enter the test tubes in the storage box and the hole at the top of the waste tank.

[0010] The reagents stored in the test tubes of the storage box are sterilized by ultraviolet disinfection tubes. Air is drawn in by an intake fan and exhausted by an exhaust fan. Cooling water is circulated in the cooling pipes of the storage box by a compressor and cooling pipes, so that the reagents stored in the test tubes of the storage box can be preserved at low temperature.

[0011] Furthermore, the unblocking mechanism includes a rigid pipe, a connecting frame, and a pump fixedly installed on the housing. The connecting frame has a through hole, the rigid pipe is connected to the inlet pipe, the connecting frame is connected to the rigid pipe, and the connecting frame is connected to the pump. The pump is equipped with a connecting pipe for connecting external reagents or clean water.

[0012] Furthermore, an electric cylinder is fixedly installed on the box body, an electromagnet is fixedly installed on the output end of the electric cylinder, a placement strip is slidably installed inside the connecting frame, the electromagnet attracts the placement strip, the placement strip is provided with multiple through holes, and a cleaning cylinder is placed in the other through holes except for the bottom through hole. The cleaning cylinder is provided with multiple cleaning scrapers, and two rows of rotating blades are provided inside the cleaning cylinder, the two rows of rotating blades are arranged alternately.

[0013] When placing the reagent, the bottom through-hole in the placement strip is aligned with the through-hole in the connecting frame. The pump is connected to the external reagent and delivers the reagent through the connecting frame, the through-hole below the placement strip, the rigid tube, and the inlet tube into the sampling tube. After the reagent is delivered, the electric cylinder extends, so that the hole with the cleaning cylinder reaches the through-hole of the connecting frame. The pump is connected to the external clean water, and the sampling tube moves above the waste tank. The sampling tube descends and is inserted into the through-hole of the waste tank. The pump delivers clean water through the connecting frame, the through-hole below the placement strip, the rigid tube, and the inlet tube into the sampling tube. At the same time, the pump simultaneously delivers the cleaning cylinder into the inlet tube. The water flow drives the cleaning cylinder to rotate through the rotating vanes. The cleaning scraper rotates and scrapes the inner wall of the rigid tube and the inlet tube, and the clean water removes the scraped impurities. The two rows of rotating vanes are arranged alternately to increase the rotation speed of the cleaning cylinder. Finally, the rinsed clean water and the cleaning cylinder enter the waste tank together through the sampling tube and are finally discharged from the drain pipe.

[0014] Furthermore, the feeding mechanism includes a feeding frame fixedly mounted on the placement plate, a feeding motor fixedly mounted on the feeding frame, a feeding lead screw rotatably mounted on the feeding frame, the feeding lead screw being fixedly mounted to the motor shaft of the feeding motor, and an isolation box fixedly mounted on the feeding frame.

[0015] Furthermore, an isolation plate is slidably installed on the isolation box, and an isolation electric cylinder is fixedly installed on the plate, with the output end of the isolation electric cylinder fixedly installed to the isolation plate.

[0016] Furthermore, an external feeding frame is slidably installed on the feeding frame, and the external feeding frame and the feeding screw form a threaded drive. A tilting motor is fixedly installed on the external feeding frame, and a tilting frame is rotatably installed on the external feeding frame. A discharge test tube is fixedly installed on the tilting frame, and the tilting frame is fixedly installed on the motor shaft of the tilting motor. The feeding frame is provided with multiple steam sterilization nozzles and multiple drainage holes. The drainage holes are connected to an external drainage pipe, and the steam sterilization nozzles are connected to an external steam generator.

[0017] When reagents are needed, the sampling tube first moves above the designated test tube, the pump reverses, and the reagent is drawn out of the test tube through the sampling tube. Then, the sampling tube moves above the discharge test tube, and the pump, in conjunction with the sampling tube, delivers the reagent into the discharge test tube. Under normal conditions, the isolation plate seals the isolation box. When reagents are needed, the delivery motor drives the delivery screw to rotate, moving the delivery frame, tilting frame, and discharge test tube outwards. When the delivery frame seals one end of the isolation box, the isolation cylinder retracts, causing the isolation plate to move away from the isolation box. The delivery motor then continues to rotate, moving the delivery frame, tilting frame, and discharge test tube to the outermost position. During this time, the delivery frame always keeps the isolation box closed, achieving continuous operation during the movement of the discharge test tube. During the process, the equipment remains in a closed and sterile state. The experimenter then removes the reagent from the discharge tube, and the delivery rack slides inward. When the discharge tube reaches above the steam sterilization nozzle, the isolation cylinder extends, causing the isolation plate to re-close the isolation box. At this point, the discharge tube is located between the delivery rack and the isolation plate. The delivery rack and the isolation plate, together with the isolation box, form a sealed space. The flipping motor drives the flipping frame and the discharge tube to rotate 180 degrees, so that the opening of the discharge tube faces downward. Steam is sprayed out through the steam sterilization nozzle to perform high-temperature steam sterilization and cleaning of the discharge tube. The cleaning water is discharged through the drain hole. Then, the flipping motor drives the flipping frame and the discharge tube to rotate 180 degrees to reset.

[0018] Furthermore, the placement plate is equipped with a detection mechanism for testing the stored reagents. The detection mechanism includes a temporary placement seat and a fixed turntable fixedly installed on the placement plate. A front end arm is rotatably installed on the fixed turntable, and a rear end arm is rotatably installed on the front end arm. A moving block is fixedly installed on the rear end arm. A magnet is fixedly installed on the temporary placement seat, and a magnetic sleeve is fixedly installed on the moving block. A lifting sleeve is slidably installed inside the moving block, and a detection probe is fixedly installed below the lifting sleeve. A compression spring is provided between the lifting sleeve and the moving block. A protective motor is fixedly installed on the storage box, and an output gear is fixedly installed on the motor shaft of the protective motor. A protective plate is rotatably installed on the storage box, and an internal gear ring is fixedly installed on the protective plate. The internal gear ring meshes with the output gear.

[0019] The protective motor drives the output gear to rotate, which in turn rotates the internal gear ring and the protective plate. The protective plate can seal the storage box. Before each use of the reagent in the storage box, the sampling tube first reaches the top of the lifting sleeve. Then, the sampling tube extends into the lifting sleeve and moves together with the lifting sleeve to the top of the reagent. The rear arm rotates relative to the front arm, and the front arm rotates relative to the fixed turntable. Then, the sampling tube continues to extend, causing the lifting sleeve and the detection probe to descend. The pressure spring is compressed, and the detection probe is inserted into the reagent to detect its acidity or alkalinity. Then, the sampling tube rises, the pressure spring rebounds, and the detection probe leaves the reagent. Then, the sampling tube, along with the lifting sleeve and the moving block, returns to the temporary placement seat and is attracted by the magnetic sleeve by the magnet.

[0020] The beneficial effects of this invention compared with the prior art are: (1) The storage mechanism set up in this invention realizes automatic sampling, storage and retrieval of reagents through precise positioning, closed conveying and ultraviolet disinfection system, without manual intervention throughout the process, and the equipment is always isolated from the outside world when it is retrieved, effectively avoiding microbial contamination and ensuring the accuracy of experimental data and the safety of reagents; (2) This invention uses a rotating cleaning cylinder, combined with a high-pressure water flow to drive a scraper to rotate and scrape the inner wall of the conveying pipe, thoroughly removing residual reagents, preventing pipe blockage and cross-contamination, and significantly improving the long-term stability and reliability of the equipment; (3) This invention uses a compressor refrigeration combined with a cooling pipe circulation system, combined with a sealed box and a temperature-controlled fan to realize constant temperature storage of reagents, reduce the impact of temperature fluctuations on microbial activity, and extend the shelf life of reagents; (4) The rotating arm transmission belt and lifting screw structure replace the complex slide rail, making the equipment structure lighter and more compact; at the same time, the delivery mechanism is integrated with the steam disinfection system to realize automatic cleaning and sterilization of the discharge test tubes, reduce manual maintenance costs and improve experimental efficiency. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0022] Figure 2 This is a schematic diagram of the storage mechanism structure of the present invention. Figure 1 .

[0023] Figure 3 This is a schematic diagram of the storage mechanism structure of the present invention. Figure 2 .

[0024] Figure 4 This is a schematic diagram of the storage mechanism structure of the present invention. Figure 3 .

[0025] Figure 5 This is a schematic diagram of the storage mechanism structure of the present invention. Figure 4 .

[0026] Figure 6 This is a schematic diagram of the unblocking mechanism of the present invention. Figure 1 .

[0027] Figure 7 This is a schematic diagram of the unblocking mechanism of the present invention. Figure 2 .

[0028] Figure 8 This is a schematic diagram of the unblocking mechanism of the present invention. Figure 3 .

[0029] Figure 9 This is a schematic diagram of the delivery mechanism of the present invention. Figure 1 .

[0030] Figure 10 This is a schematic diagram of the delivery mechanism of the present invention. Figure 2 .

[0031] Figure 11 This is a schematic diagram of the detection mechanism of the present invention. Figure 1 .

[0032] Figure 12 This is a schematic diagram of the detection mechanism of the present invention. Figure 2 .

[0033] Figure 13 This is a schematic diagram of the detection mechanism of the present invention. Figure 3 .

[0034] Reference numerals: 101-Box body; 102-Box door; 103-Storage box; 104-Intake fan; 105-Exhaust fan; 106-Base wheel; 107-Compressor; 108-Cooling pipe; 109-UV disinfection pipe; 110-Placement plate; 111-Fixed base; 112-Swing arm motor; 113-Swing arm belt; 114-Rotating column; 115-Rotating arm motor; 116-Motor gear; 117-Rotating arm; 118-Rotating arm gear; 119-Rotating arm transmission belt; 120-Front end arm; 121-Lifting motor; 122-Lifting belt; 123-Lead screw; 124-Lifting seat; 125-Sampling tube; 126-Waste tank; 127-Drainage pipe; 128-Column; 201-Inlet pipe; 202-Hard pipe; 203-Pump; 204-Electric cylinder ; 205-Electromagnet; 206-Placement bar; 207-Cleaning cylinder; 208-Cleaning scraper; 209-Rotating plate; 210-Connecting frame; 301-Feeding frame; 302-Isolation electric cylinder; 303-Isolation plate; 304-Isolation box; 305-Feeding motor; 306-Feeding screw; 307-External feeding frame; 308-Tilting motor; 309-Tilting frame; 310-Discharge test tube; 311-Steam sterilization nozzle; 401-Temporary placement seat; 402-Fixed turntable; 403-Front end arm II; 404-Rear end arm; 405-Lifting sleeve; 406-Detection probe; 407-Compression spring; 408-Moving block; 409-Magnetic sleeve; 410-Magnet; 411-Protective motor; 412-Output gear; 413-Internal gear ring; 414-Protective plate. Detailed Implementation

[0035] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings.

[0036] Example: Reference Figures 1-10 An automated aseptic sampling and low-temperature preservation integrated device includes a storage mechanism for storing and retrieving reagents. The storage mechanism includes a housing 101. The storage mechanism is equipped with a cleaning mechanism for cleaning pipes to avoid blockage and a delivery mechanism for delivering reagents. The cleaning mechanism includes an inlet pipe 201 fixedly installed on the housing 101.

[0037] The storage mechanism includes a placement plate 110 fixedly installed inside the housing 101, a storage box 103 fixedly installed on the placement plate 110, a plurality of test tubes for storing reagents are provided inside the storage box 103, and a cooling pipe is provided inside the storage box 103.

[0038] like Figures 2-5 As shown, the storage mechanism also includes four casters 106 at the bottom of the box 101, an ultraviolet disinfection tube 109 inside the box 101, an intake fan 104 and an exhaust fan 105 on the box 101, a compressor 107 fixedly installed inside the box 101, a door 102 rotatably installed on the box 101, a cooling pipe 108 fixedly installed on the compressor 107, and the other end of the cooling pipe 108 connected to a cooling pipe inside the storage box 103.

[0039] like Figures 2-5 As shown, a fixed base 111 is fixedly installed on the placement plate 110. A rotating column 114 is rotatably installed inside the fixed base 111. A swing arm motor 112 is fixedly installed on the fixed base 111. The swing arm motor 112 drives the rotating column 114 to rotate through the swing arm belt 113. A rotating arm motor 115 is fixedly installed on the rotating column 114. A motor gear 116 is fixedly installed on the motor shaft of the rotating arm motor 115. A rotating arm 117 is rotatably installed on the rotating column 114. A rotating arm gear 118 is fixedly installed on the rotating arm 117. The rotating arm gear 118 meshes with the motor gear 116. A front arm 120 is rotatably installed on the rotating arm 117. A column 128 is fixedly installed on the front arm 120. A rotating arm transmission belt 119 is wound around the rotating column 114 and the column 128.

[0040] like Figures 2-5 As shown, a lifting motor 121 is fixedly installed on the column 128, a vertical lead screw 123 is rotatably installed inside the front arm 120, and a lifting seat 124 is slidably installed inside the front arm 120. The lifting seat 124 and the vertical lead screw 123 form a threaded transmission. A sampling tube 125 is fixedly installed below the lifting seat 124. The lifting seat 124 is connected to the inlet pipe 201. The lifting motor 121 drives the vertical lead screw 123 to rotate through the lifting belt 122.

[0041] like Figures 2-5 As shown, a waste tank 126 is fixedly installed on the housing 101. The top of the waste tank 126 has a hole with a diameter larger than the outer diameter of the sampling tube 125. A sewage pipe 127 is fixedly installed below the waste tank 126 and is connected to an external sewage pipe.

[0042] The reagent to be stored is pumped by pump 203 through inlet pipe 201 to lifting seat 124, and finally enters the test tube in storage box 103 through sampling tube 125. Rotating arm motor 115 drives motor gear 116 to rotate, which in turn drives rotating arm gear 118 and rotating arm motor 115 to rotate. As rotating arm 117 rotates, it drives front arm 120 to move together. When rotating column 114 does not rotate, rotating arm transmission belt 119 drives column 128 and front arm 120 to rotate, so that sampling tube 125 always moves along the horizontal line connecting the axis of sampling tube 125 and the axis of rotating column 114. Swing arm motor 112 drives the swing arm The drive column 114 rotates relative to the fixed base 111 via the belt 113. Through the combination of the two actions, the sampling tube 125 can reach above all the test tubes in the storage box 103, and the sampling tube 125 can reach above the waste tank 126 and the discharge test tube 310. The complex slide rail structure is replaced by the rotating arm drive belt 119 and the front arm 120, making the equipment lighter. The lifting motor 121 drives the vertical screw 123 to rotate via the lifting belt 122, which drives the lifting base 124 and the sampling tube 125 to rise and fall, so that the sampling tube 125 can enter the test tubes in the storage box 103 and the hole at the top of the waste tank 126.

[0043] The reagents stored in the test tubes of the storage box 103 are sterilized by the ultraviolet disinfection tube 109. Air is introduced by the intake fan 104 and discharged by the exhaust fan 105. Cooling water is circulated in the cooling pipe of the storage box 103 by the compressor 107 and the cooling pipe 108, so that the reagents stored in the test tubes of the storage box 103 can be preserved at low temperature.

[0044] like Figures 6-8 As shown, the unblocking mechanism includes a rigid pipe 202, a connecting frame 210, and a pump 203, which are fixedly installed on the housing 101. The connecting frame 210 has a through hole. The rigid pipe 202 is connected to the inlet pipe 201. The connecting frame 210 is connected to the rigid pipe 202 and the pump 203. The pump 203 is provided with a connecting pipe for connecting external reagents or clean water.

[0045] like Figures 6-8 As shown, an electric cylinder 204 is fixedly installed on the housing 101, and an electromagnet 205 is fixedly installed on the output end of the electric cylinder 204. A placement strip 206 is slidably installed inside the connecting frame 210. The electromagnet 205 attracts the placement strip 206. The placement strip 206 is provided with multiple through holes. Except for the bottom through hole, the other through holes are filled with cleaning cylinders 207. Multiple cleaning scrapers 208 are provided on the cleaning cylinder 207. Two rows of rotating blades 209 are provided inside the cleaning cylinder 207. The two rows of rotating blades 209 are arranged alternately.

[0046] When placing the reagent, the lowest through-hole in the placement strip 206 is aligned with the through-hole in the connecting frame 210. The pump 203 is connected to the external reagent and pumps the reagent through the connecting frame 210, the through-hole below the placement strip 206, the rigid tube 202, and the inlet tube 201 into the sampling tube 125. After the reagent is placed, the electric cylinder 204 extends, so that the hole with the cleaning cylinder 207 reaches the through-hole of the connecting frame 210. The pump 203 is connected to the external clean water, and the sampling tube 125 moves above the waste tank 126. The sampling tube 125 descends and inserts into the through-hole of the waste tank 126. The pump 203 pumps clean water through the connecting frame. 210. The through hole, rigid pipe 202 and inlet pipe 201 below the placement strip 206 are sent into the sampling pipe 125. At the same time, the pump 203 simultaneously sends the cleaning cylinder 207 into the inlet pipe 201. The water flow drives the cleaning cylinder 207 to rotate through the rotating blade 209. The cleaning scraper 208 rotates and scrapes the inner wall of the rigid pipe 202 and the inlet pipe 201. The scraped impurities are removed with the help of clean water. The two rows of rotating blades 209 are arranged alternately to increase the rotation speed of the cleaning cylinder 207. Finally, the rinse water and the cleaning cylinder 207 enter the waste tank 126 through the sampling pipe 125 and are finally discharged from the drain pipe 127.

[0047] like Figure 9 , Figure 10 As shown, the feeding mechanism includes a feeding frame 301 fixedly installed on the placement plate 110, a feeding motor 305 fixedly installed on the feeding frame 301, a feeding screw 306 rotatably installed on the feeding frame 301, the feeding screw 306 being fixedly installed with the motor shaft of the feeding motor 305, and an isolation box 304 fixedly installed on the feeding frame 301.

[0048] like Figure 9 , Figure 10 As shown, an isolation plate 303 is slidably installed on the isolation box 304, and an isolation electric cylinder 302 is fixedly installed on the placement plate 110. The output end of the isolation electric cylinder 302 is fixedly installed with the isolation plate 303.

[0049] like Figure 9 , Figure 10 As shown, an external delivery frame 307 is slidably installed on the delivery frame 301. The external delivery frame 307 and the delivery screw 306 form a threaded drive. A tilting motor 308 is fixedly installed on the external delivery frame 307. A tilting frame 309 is rotatably installed on the external delivery frame 307. A discharge test tube 310 is fixedly installed on the tilting frame 309. The tilting frame 309 is fixedly installed with the motor shaft of the tilting motor 308. The delivery frame 301 is provided with multiple steam sterilization nozzles 311 and multiple drain holes. The drain holes are connected to an external drain pipe, and the steam sterilization nozzles 311 are connected to an external steam generator.

[0050] When reagents are needed, the sampling tube 125 first moves above the designated test tube, the pump 203 reverses, and the reagent in the test tube is drawn out through the sampling tube 125. Then, the sampling tube 125 moves above the discharge test tube 310, and the pump 203, in conjunction with the sampling tube 125, delivers the reagent into the discharge test tube 310. Under normal conditions, the isolation plate 303 closes the isolation box 304. When reagents are needed, the delivery motor 305 drives the delivery screw 306 to rotate, causing the delivery frame 307, the flipping frame 309, and the discharge test tube 310 to move outward. When the delivery frame 307 seals one end of the isolation box 304, the isolation cylinder 302 retracts, causing the isolation plate 303 to leave the isolation box 304. Then, the delivery motor 305 continues to rotate, causing the delivery frame 307, the flipping frame 309, and the discharge test tube 310 to move to the outermost position. At this time, the delivery frame 307 always keeps the isolation box 304 closed, achieving the desired effect in the discharge test tube. During the movement of 310, the equipment remains in a closed and sterile state. Subsequently, the experimenter removes the reagent from the discharge tube 310. Then, the external delivery rack 307 slides inward. When the discharge tube 310 reaches above the steam sterilization nozzle 311, the isolation cylinder 302 extends, causing the isolation plate 303 to re-close the isolation box 304. At this time, the discharge tube 310 is located between the external delivery rack 307 and the isolation plate 303. The external delivery rack 307 and the isolation plate 303, together with the isolation box 304, form a sealed space. The flipping motor 308 drives the flipping frame 309 and the discharge tube 310 to rotate 180 degrees, so that the opening of the discharge tube 310 faces downward. Steam is sprayed out through the steam sterilization nozzle 311 to perform high-temperature steam sterilization and cleaning of the discharge tube 310. The cleaning water is discharged through the drain hole. Then, the flipping motor 308 drives the flipping frame 309 and the discharge tube 310 to rotate 180 degrees to reset.

[0051] like Figures 11-13 As shown, the placement plate 110 is equipped with a detection mechanism for testing the stored reagents. The detection mechanism includes a temporary placement seat 401 and a fixed turntable 402 fixedly installed on the placement plate 110. A front end arm 403 is rotatably mounted on the fixed turntable 402, and a rear end arm 404 is rotatably mounted on the front end arm 403. A moving block 408 is fixedly mounted on the rear end arm 404. A magnet 410 is fixedly mounted on the temporary placement seat 401, and a magnetic sleeve 409 is fixedly mounted on the moving block 408. A lifting sleeve 405 is slidably installed inside the moving block 408. A detection probe 406 is fixedly installed below the lifting sleeve 405. A compression spring 407 is provided between the lifting sleeve 405 and the moving block 408. A protective motor 411 is fixedly mounted on the storage box 103. An output gear 412 is fixedly mounted on the motor shaft of the protective motor 411. A protective plate 414 is rotatably mounted on the storage box 103. An internal gear ring 413 is fixedly mounted on the protective plate 414. The internal gear ring 413 meshes with the output gear 412.

[0052] The protective motor 411 drives the output gear 412 to rotate, which in turn drives the internal gear ring 413 and the protective plate 414 to rotate. The protective plate 414 can seal the storage box 103. Before each use of the reagent in the storage box 103, the sampling tube 125 first reaches above the lifting sleeve 405. Then, the sampling tube 125 extends into the lifting sleeve 405. Subsequently, the sampling tube 125 moves together with the lifting sleeve 405 above the reagent. The rear arm 404 rotates relative to the front arm 403, and the front arm 403 rotates relative to the front arm 403. As the fixed turntable 402 rotates, the sampling tube 125 continues to extend, causing the lifting sleeve 405 and the detection probe 406 to descend. The pressure spring 407 is compressed, and the detection probe 406 is inserted into the reagent to detect its acidity or alkalinity. Then, the sampling tube 125 rises, and the pressure spring 407 rebounds, causing the detection probe 406 to leave the reagent. Subsequently, the sampling tube 125, along with the lifting sleeve 405 and the moving block 408, returns to the temporary seat 401, where the magnetic sleeve 409 is attracted by the magnet 410.

[0053] The working principle of the automated aseptic sampling and cryogenic preservation integrated device disclosed in this invention is as follows: When placing reagents, the lowermost through hole in the placement strip 206 is aligned with the through hole in the connecting frame 210. The pump 203 is connected to the external reagent. The pump 203 delivers the reagent through the connecting frame 210, the through hole below the placement strip 206, the rigid tube 202, and the inlet tube 201 into the sampling tube 125. The pump 203 transports the reagent to be stored through the inlet tube 201 to the lifting seat 124, and finally through the sampling tube 125 into the test tube in the storage box 103. The rotating arm motor 115 drives the motor gear 116 to rotate, which in turn drives the rotating arm gear 118 and the rotating arm motor 115 to rotate. Since the rotating arm 117 rotates, it drives the front arm 120 to move together. When the rotating column 114 does not rotate, the rotating arm transmission belt 119 carries... The rotating column 128 and the front arm 120 rotate, causing the sampling tube 125 to always move along the horizontal line connecting the axis of the sampling tube 125 and the axis of the rotating column 114. The swing arm motor 112 drives the rotating column 114 to rotate relative to the fixed seat 111 through the swing arm belt 113. Through the combination of the two actions, the sampling tube 125 can reach above all the test tubes in the storage box 103, and the sampling tube 125 can reach above the waste tank 126 and the discharge test tube 310. The rotating arm transmission belt 119 and the front arm 120 replace the complex slide rail structure, making the equipment lighter. The lifting motor 121 drives the vertical screw 123 to rotate through the lifting belt 122, driving the lifting seat 124 and the sampling tube 125 to rise and fall, so that the sampling tube 125 can enter the test tubes in the storage box 103 and the hole at the top of the waste tank 126. The reagents stored in the test tubes of the storage box 103 are sterilized by ultraviolet disinfection tube 109. Air is drawn in by intake fan 104 and exhausted by exhaust fan 105. Cooling water circulates in the cooling pipe of the storage box 103 through compressor 107 and cooling pipe 108, enabling the reagents stored in the test tubes of the storage box 103 to be preserved at low temperature. After the reagents are fed in, electric cylinder 204 extends, so that the hole with cleaning tube 207 reaches the through hole of connecting rack 210. Pump 203 is connected to external clean water. Sampling tube 125 moves above waste tank 126 and descends to insert into the through hole of waste tank 126. Pump 203 delivers clean water into sampling tube 125 through connecting rack 210, through the through hole below placement bar 206, rigid tube 202 and inlet tube 201. At the same time, pump 203 simultaneously delivers clean water into the sampling tube 125. The cleaning cylinder 207 is fed into the inlet pipe 201. The water flow drives the cleaning cylinder 207 to rotate through the rotating blades 209. The cleaning scraper 208 rotates and scrapes the inner wall of the rigid pipe 202 and the inlet pipe 201. The scraped impurities are removed with the help of clean water. The two rows of rotating blades 209 are arranged alternately to increase the rotation speed of the cleaning cylinder 207. Finally, the clean water and the cleaning cylinder 207 enter the waste tank 126 through the sampling pipe 125 and are finally discharged from the drain pipe 127.When reagents need to be retrieved, the protective motor 411 drives the output gear 412 to rotate, which in turn rotates the internal gear ring 413 and the protective plate 414. The protective plate 414 can then seal the storage box 103. Before each retrieval of reagents from the storage box 103, the sampling tube 125 first reaches above the lifting sleeve 405. Then, the sampling tube 125 extends into the lifting sleeve 405, and subsequently moves the sampling tube 125, along with the lifting sleeve 405, above the reagents. The rear arm 404 rotates relative to the front arm 403, and the front arm 403 rotates relative to the fixed turntable 402. Then, the sampling tube 125 continues to extend, causing the lifting sleeve 405 and the detection probe 406 to descend, compressing the pressure spring 407. The detection probe 406 is inserted into the reagent to detect its acidity or alkalinity. Then, the sampling tube 125 rises, and the spring 407 returns, causing the detection probe 406 to leave the reagent. The sampling tube 125, along with the lifting sleeve 405 and the moving block 408, returns to the temporary placement seat 401, where it is attracted by the magnetic sleeve 409 via the magnet 410. The sampling tube 125 then moves above the designated test tube, and the pump 203 reverses, drawing the reagent out of the test tube through the sampling tube 125. The sampling tube 125 then moves above the discharge test tube 310, and the pump 203, in conjunction with the sampling tube 125, delivers the reagent into the discharge test tube 310. Under normal conditions, the isolation plate 303 seals the isolation box 304. When retrieving the reagent... The delivery motor 305 drives the delivery screw 306 to rotate, causing the delivery frame 307, the flipping frame 309, and the discharge test tube 310 to move outward. When the delivery frame 307 seals one end of the isolation box 304, the isolation cylinder 302 retracts, causing the isolation plate 303 to move away from the isolation box 304. Then, the delivery motor 305 continues to rotate, moving the delivery frame 307, the flipping frame 309, and the discharge test tube 310 to the outermost position. At this time, the delivery frame 307 keeps the isolation box 304 closed, ensuring that the equipment remains in a closed and sterile state during the movement of the discharge test tube 310. Subsequently, the experimenter removes the reagent from the discharge test tube 310. Then, the delivery frame 307 slides inward, and when the discharge test tube 310... Upon reaching the steam sterilization nozzle 311, the isolation cylinder 302 extends, causing the isolation plate 303 to re-close the isolation box 304. At this time, the discharge test tube 310 is located between the delivery frame 307 and the isolation plate 303. The delivery frame 307 and the isolation plate 303, together with the isolation box 304, form a sealed space. The flipping motor 308 drives the flipping frame 309 and the discharge test tube 310 to rotate 180 degrees, so that the opening of the discharge test tube 310 faces downward. Steam is sprayed out through the steam sterilization nozzle 311 to perform high-temperature steam sterilization and cleaning of the discharge test tube 310. The cleaning water is discharged through the drain hole. Then, the flipping motor 308 drives the flipping frame 309 and the discharge test tube 310 to rotate 180 degrees to reset.

[0054] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the present invention based on the technical solution and inventive concept of the present invention should be covered within the scope of protection of the present invention.

Claims

1. An automated aseptic sampling and cryopreservation integrated device, comprising a storage mechanism for storing and retrieving reagents, characterized in that: The storage mechanism includes a housing (101), and the storage mechanism is provided with a cleaning mechanism for cleaning the pipes to avoid blockage and a delivery mechanism for delivering reagents. The cleaning mechanism includes an inlet pipe (201) fixedly installed on the housing (101). The storage mechanism includes a placement plate (110) fixedly installed inside the box (101), a storage box (103) fixedly installed on the placement plate (110), a plurality of test tubes for storing reagents are provided inside the storage box (103), and a cooling pipe is provided inside the storage box (103). The storage mechanism also includes four bottom wheels (106) set at the bottom of the box (101), an ultraviolet disinfection tube (109) is set inside the box (101), an intake fan (104) and an exhaust fan (105) are set on the box (101), a compressor (107) is fixedly installed inside the box (101), a box door (102) is rotatably installed on the box (101), a cooling pipe (108) is fixedly installed on the compressor (107), and the other end of the cooling pipe (108) is connected to the cooling pipe inside the storage box (103); A fixed base (111) is fixedly installed on the placement plate (110). A rotating column (114) is rotatably installed inside the fixed base (111). A swing arm motor (112) is fixedly installed on the fixed base (111). The swing arm motor (112) drives the rotating column (114) to rotate through the swing arm belt (113). A rotating arm motor (115) is fixedly installed on the rotating column (114). A motor gear (116) is fixedly installed on the motor shaft of the rotating arm motor (115). A rotating arm (117) is rotatably installed on the rotating column (114). A rotating arm gear (118) is fixedly installed on the rotating arm (117). The rotating arm gear (118) meshes with the motor gear (116). A front arm (120) is rotatably installed on the rotating arm (117). A column (128) is fixedly installed on the front arm (120). A rotating arm transmission belt (119) is wound around the rotating column (114) and the column (128). A lifting motor (121) is fixedly installed on the column (128), a vertical screw (123) is rotatably installed inside the front arm (120), and a lifting seat (124) is slidably installed inside the front arm (120). The lifting seat (124) and the vertical screw (123) form a threaded transmission. A sampling tube (125) is fixedly installed below the lifting seat (124). The lifting seat (124) is connected to the inlet pipe (201). The lifting motor (121) drives the vertical screw (123) to rotate through the lifting belt (122). The unblocking mechanism includes a rigid pipe (202), a connecting frame (210), and a pump (203) fixedly installed on the housing (101). The connecting frame (210) has a through hole. The rigid pipe (202) is connected to the inlet pipe (201). The connecting frame (210) is connected to the rigid pipe (202). The connecting frame (210) is connected to the pump (203). The pump (203) is equipped with a connecting pipe for connecting external reagents or clean water. An electric cylinder (204) is fixedly installed on the housing (101). An electromagnet (205) is fixedly installed on the output end of the electric cylinder (204). A placement strip (206) is slidably installed inside the connecting frame (210). The electromagnet (205) attracts the placement strip (206). The placement strip (206) has multiple through holes. Except for the bottom through hole, the other through holes contain cleaning cylinders (207). The cleaning cylinder (207) has multiple cleaning scrapers (208). The cleaning cylinder (207) contains two rows of rotating blades (209). The two rows of rotating blades (209) are arranged alternately.

2. The automated aseptic sampling and cryopreservation integrated device according to claim 1, characterized in that: A waste tank (126) is fixedly installed on the box (101). The top of the waste tank (126) has a hole with a diameter larger than the outer diameter of the sampling tube (125). A sewage pipe (127) is fixedly installed below the waste tank (126) and is connected to an external sewage pipe.

3. The automated aseptic sampling and cryopreservation integrated device according to claim 1, characterized in that: The delivery mechanism includes a delivery frame (301) fixedly installed on the placement plate (110), a delivery motor (305) fixedly installed on the delivery frame (301), a delivery screw (306) rotatably installed on the delivery frame (301), the delivery screw (306) being fixedly installed with the motor shaft of the delivery motor (305), and an isolation box (304) fixedly installed on the delivery frame (301).

4. The automated aseptic sampling and cryopreservation integrated device according to claim 3, characterized in that: An isolation plate (303) is slidably installed on the isolation box (304), and an isolation electric cylinder (302) is fixedly installed on the placement plate (110). The output end of the isolation electric cylinder (302) is fixedly installed on the isolation plate (303). An external delivery frame (307) is slidably installed on the delivery frame (301). The external delivery frame (307) and the delivery screw (306) form a threaded drive. A flip motor (308) is fixedly installed on the external delivery frame (307). A flip frame (309) is rotatably installed on the external delivery frame (307). A discharge test tube (310) is fixedly installed on the flip frame (309). The flip frame (309) is fixedly installed on the motor shaft of the flip motor (308). The delivery frame (301) is provided with multiple steam sterilization nozzles (311) and multiple drainage holes. The drainage holes are connected to the external drainage pipe, and the steam sterilization nozzles (311) are connected to the external steam engine.

5. The automated aseptic sampling and cryopreservation integrated device according to claim 1, characterized in that: The placement plate (110) is provided with a detection mechanism for detecting the stored reagents. The detection mechanism includes a temporary placement seat (401) and a fixed turntable (402) fixedly installed on the placement plate (110). A front end arm (403) is rotatably installed on the fixed turntable (402), and a rear end arm (404) is rotatably installed on the front end arm (403). A moving block (408) is fixedly installed on the rear end arm (404). A magnet (410) is fixedly installed on the temporary placement seat (401), and a magnetic sleeve (409) is fixedly installed on the moving block (408). 08) A lifting sleeve (405) is slidably installed inside. A detection probe (406) is fixedly installed below the lifting sleeve (405). A compression spring (407) is provided between the lifting sleeve (405) and the moving block (408). A protective motor (411) is fixed on the storage box (103). An output gear (412) is fixedly installed on the motor shaft of the protective motor (411). A protective plate (414) is rotatably installed on the storage box (103). An internal gear ring (413) is fixedly installed on the protective plate (414). The internal gear ring (413) meshes with the output gear (412).

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