Device and method for extracting and purifying antibody

Abstract

The invention belongs to the technical field of purification devices, and discloses an antibody extraction and purification device and a method thereof.The antibody extraction and purification device comprises a device body and a stirring barrel installed at the upper end, a discharging pipe is connected to the upper end of the left side of the device body, a filtering hopper is arranged at the lower end of the stirring barrel, and a filtering box is arranged at the lower end of the filtering hopper; the stirring barrel is fixed to the inner wall of the device body, a stirring motor is fixed to the upper end of the stirring barrel, a transmission shaft is connected to the lower shaft end of the stirring motor, a stirring plate is connected to the outer wall of the transmission shaft, a limiting plate is fixed to the upper end of the filter hopper, a filter screen is connected into the filter hopper, and power motors are fixed to the two sides of the device body; a transmission disc is fixed to the inner side end of the power motor, a transmission rod is fixed to the inner side of the transmission disc, guide frames are fixed to the two sides of the filter hopper, a positioning frame is arranged in the filter box, an ultrafiltration membrane is installed in the positioning frame, guide blocks are fixed to the upper ends of the two sides of the positioning frame, and limiting protruding blocks are connected to the outer wall of the transmission disc.

Description

Technical Field

[0001] This invention relates to the field of purification equipment technology, specifically to an apparatus and method for the extraction and purification of antibodies. Background Technology

[0002] Antibodies are large Y-shaped proteins secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. They are only found in the body fluids of vertebrates, such as blood, and on the cell membrane surface of their B cells. Antibodies can recognize a unique feature of a specific foreign substance, which is called an antigen. During the production process, antibodies contain some visible particles, macromolecular impurities (such as HCP, DNA, viruses, and aggregates), and some small molecule contaminants. They need to be filtered and purified during the production process, which requires the use of purification equipment.

[0003] However, existing equipment and methods for antibody extraction and purification still have certain problems in use: A high-throughput antibody purification device, as described in Chinese Patent Application No. CN202022123462.6, includes a chromatography column, a sealing plug on the upper side of the chromatography column, a filter screen installed inside the chromatography column, a first sleeve ring sleeved on the outer side of the chromatography column, and a second sleeve ring hinged to the left side of the first sleeve ring. A hinge plate is hinged to the upper side of the first sleeve ring, and a receiving groove is formed on the lower side of the hinge plate. A pull rod is horizontally inserted inside the receiving groove, and an insert is fixedly connected to the right side of the pull rod. A spring is sleeved on the outer surface of the pull rod. However, existing antibody purification devices rely solely on a simple filtration structure for filtration, and rely solely on the gravity of the raw materials for filtration, resulting in low filtration efficiency and unsatisfactory filtration effects.

[0004] To address the aforementioned issues, an innovative design was developed based on existing antibody extraction and purification devices and methods. Summary of the Invention

[0005] The purpose of this invention is to provide an apparatus and method for the extraction and purification of antibodies, in order to solve the problem mentioned in the background art that existing antibody purification devices rely on only a single filtration structure, resulting in low filtration efficiency and unsatisfactory filtration effect.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an apparatus and method for antibody extraction and purification, comprising a main body and a stirring tank mounted on the upper end, a feed pipe connected to the upper left side of the main body, a filter bucket disposed at the lower end of the stirring tank, and a filter box disposed at the lower end of the filter bucket. The mixing tank is fixed to the inner wall of the main body of the device, and a stirring motor is fixed at the upper end of the mixing tank. A transmission shaft is connected to the lower shaft end of the stirring motor, and a stirring plate is connected to the outer wall of the transmission shaft. A limit plate is fixed at the upper end of the filter bucket, and a filter screen is connected inside the filter bucket. Power motors are fixed on both sides of the main body of the device, and a transmission disc is fixed on the inner end of the power motor, and a transmission rod is fixed on the inner side of the transmission disc. Guide frames are fixed on both sides of the filter bucket. The filter box is equipped with a positioning frame, and an ultrafiltration membrane is installed inside the positioning frame. Guide blocks are fixed at the upper ends of both sides of the positioning frame, and limit protrusions are connected to the outer wall of the transmission disk.

[0007] Preferably, the lower end of the mixing tank is designed in a funnel shape, and the mixing tank is fixed to the inner wall of the main body of the device. The end of the mixing plate is in contact with the inner wall of the mixing tank, and the mixing plates are arranged in an array with the drive shaft as the axis.

[0008] By adopting the above technical solution, through the design of the stirring tank and stirring structure, stirring can be achieved during the antibody extraction and purification process. The drive shaft is driven by a power motor to rotate, and the drive shaft drives multiple sets of stirring plates to rotate, thereby stirring the materials, dispersing and mixing them to improve the efficiency of subsequent filtration and purification.

[0009] Preferably, the lower end of the drive shaft is connected to an auger shaft, and the auger shaft is located on the lower side of the mixing tank, and the auger shaft is adapted to the inner wall of the lower end of the mixing tank.

[0010] By adopting the above technical solution, the cooperation between the auger shaft and the drive shaft can provide rotational power to the auger shaft when the drive shaft rotates. After the mixing is completed, the auger shaft can be driven to rotate in the opposite direction and the material can be fed out by the auger shaft, which improves the convenience and efficiency of feeding.

[0011] Preferably, a one-way rotating rod is rotatably connected inside the feeding pipe, and a limiting plate is fixed on the outer wall of the one-way rotating rod. The limiting plate is adapted to the inner wall of the feeding pipe. The right side of the one-way rotating rod extends outside the feeding pipe, and a driven bevel gear is fixed at the right end of the one-way rotating rod. The lower end of the stirring motor is connected to a driving bevel gear, and the driving bevel gear and the driven bevel gear are meshed.

[0012] By adopting the above technical solution, through the cooperation of the active bevel gear and the driven bevel gear, the active bevel gear can drive the driven bevel gear and the one-way rotating rod to rotate when the stirring motor rotates, and the one-way rotating rod can drive the limiting plate to rotate to realize material feeding. The limiting plate and the driven bevel gear are connected by a one-way rotating rod, which can realize the one-way transmission between the stirring motor and the limiting plate.

[0013] Preferably, a limiting plate is fixed at the upper end of the filter bucket, a through hole is opened in the middle of the limiting plate, and the lower end of the stirring bucket extends into the through hole. Guide rods are passed through both sides of the filter bucket, and the guide rods are slidably connected to the filter bucket, and the two ends of the guide rods are fixed to the inner wall of the main body of the device.

[0014] Using the above technical solution, the filter bucket can be supported by two sets of guide rods, enabling the filter bucket to move horizontally and maintain the stability of horizontal movement. The through hole in the middle of the limiting plate can prevent interference between the filter bucket and the mixing tank during reciprocating movement.

[0015] Preferably, the transmission rod is connected to the edge of the transmission disk, the guide frame is a rectangular frame design, and the transmission rod is slidably connected to the inner wall of the guide frame.

[0016] By adopting the above technical solution, the transmission disc and the transmission rod are connected off-axis. When the transmission disc rotates, it can drive the transmission rod to make a circular motion. When the transmission rod rotates, it moves inside the rectangular guide frame and drives the guide frame and the filter bucket to reciprocate.

[0017] Preferably, a baffle is provided at the lower end of the filter bucket, and the lower end of the filter bucket passes through the lower end of the baffle. A through hole is opened in the middle of the baffle. Sealing plates are fixed on both sides of the filter bucket, and the sealing plates pass through the interior of the baffle. The baffle is slidably connected to the interior of the through hole, and the transmission disc passes through the lower end of the baffle.

[0018] By adopting the above technical solution, the filter bucket and the filter box below can be separated by the baffle, and the sealing plate and the filter bucket can be used to maintain the seal between the filter bucket and the sealing plate when the filter bucket is reciprocating.

[0019] Preferably, the positioning frame is slidably connected to the inner wall of the filter box, and a reset device is connected to the lower ends of both sides of the positioning frame. The lower ends of the reset devices are fixed to the inner wall of the filter box. Guide blocks are fixed to the upper ends of both sides of the positioning frame. Limiting protrusions are connected to the outer wall of the transmission disk. The limiting protrusions are arranged in a circular array around the center of the transmission disk, and the outer wall of the limiting protrusions contacts the upper end of the guide block.

[0020] By adopting the above technical solution, through the cooperation of the positioning frame and the filter box, when the transmission disc drives the limiting protrusion to rotate, the limiting protrusion can continuously push the guide block, positioning frame and ultrafiltration membrane up and down during rotation. In conjunction with the resetter, the positioning frame is provided with upward reset power, thereby ensuring that the ultrafiltration structure can achieve up and down reciprocating vibration when the transmission disc and the limiting protrusion rotate, thus improving the filtration and purification efficiency.

[0021] Preferably, guide rods are fixed on both sides of the filter bucket, and airbags are fixed on the outer side of the guide rods. Two sets of airbags are symmetrically arranged front and back, and a connecting pipe is connected to the middle of the left side of the airbag. An air outlet one-way valve is connected to the middle of the connecting pipe. A hose is connected to the lower end of the air outlet one-way valve, and a conduit is connected to the lower end of the hose. The lower end of the conduit passes through a baffle plate. The outer end of the airbag passes through the outside of the main body of the device, and an air inlet one-way valve is connected to the outer end of the airbag. A dust cover is provided on the outside of the air inlet one-way valve, and the dust cover is fixed to the outer wall of the main body of the device.

[0022] By adopting the above technical solution, the reciprocating motion of the filter bucket, combined with the airbag structure, can pressurize the area below the sealing plate, continuously inflating and pressurizing the lower end of the sealing plate to further improve the filtration efficiency. During the reciprocating motion of the filter bucket, the guide rod continuously compresses the airbag and continuously draws in and delivers external air to the lower end of the sealing plate.

[0023] An apparatus for antibody extraction and purification includes the following steps: Step 1: The antibody raw material is fed at a uniform rate and in a quantitative manner, and stirring is carried out simultaneously during the feeding process. Step 2: After the antibody is fed into the container, continue stirring to disperse it. After stirring, further feed the antibody raw materials. Step 3: After stirring and dispersing, the raw materials are pre-filtered to remove larger debris and impurities; Step 4: After removing some impurities, perform final filtration using an ultrafiltration system, and store the purified antibody raw material. Compared with the prior art, the beneficial effects of the present invention are as follows: the antibody extraction and purification device and method, by setting up a multi-layer filtration structure, can achieve pre-filtration of antibody raw materials during ultrafiltration. Pre-filtration can remove large particulate impurities, microorganisms, fine particles, and residual cells, and remove larger residues, avoiding increasing the probability of clogging during ultrafiltration. In addition, the reciprocating structure and the stamping structure can improve the filtration and purification efficiency of antibody raw materials.

[0024] 1. The design of the mixing tank and mixing structure is as follows: the power motor drives the transmission shaft to rotate, which drives multiple sets of mixing plates to rotate and disperse the mixed materials, providing a good foundation for subsequent filtration and purification, and improving the filtration and purification efficiency. It is also equipped with an auger shaft and transmission shaft structure, which provides power to the auger shaft when the transmission shaft rotates. After the mixing is completed, the auger shaft can be driven to rotate in the opposite direction to drive the material to be discharged, which improves the convenience and efficiency of material discharge. 2. As the core component of the purification device, the filter box features a sealed design at the bottom, allowing for up-and-down movement during antibody raw material processing. Combined with a pressurized structure, this dynamic processing method effectively improves ultrafiltration efficiency. Ultrafiltration can remove molecular impurities (such as HCP, DNA, viruses, and aggregates) and some small molecule contaminants while preserving the integrity and activity of the target antibody. Furthermore, the filter box is detachable, facilitating subsequent cleaning and maintenance by the user, further enhancing the practicality and durability of the device. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the mixing tank and filter bucket structure of the present invention; Figure 3This is a cross-sectional view of the mixing tank of the present invention; Figure 4 This is a schematic diagram of the filter bucket and filter screen structure of the present invention; Figure 5 This is a schematic diagram of the transmission disc and limiting protrusion structure of the present invention; Figure 6 This is a schematic diagram of the positioning frame and ultrafiltration membrane structure of the present invention; Figure 7 This is a schematic diagram of the filter bucket and guide rod structure of the present invention; Figure 8 This is a schematic diagram of the unidirectional rotating rod and the limiting plate structure of the present invention; Figure 9 This is a schematic diagram of the airbag and guide rod structure of the present invention.

[0026] In the diagram: 1. Main body of the device; 2. Mixing tank; 3. Feed pipe; 4. Mixing motor; 5. Drive shaft; 6. Mixing plate; 7. Screw shaft; 8. One-way rotating rod; 9. Material limiting plate; 10. Driven bevel gear; 11. Driving bevel gear; 12. Filter hopper; 13. Limiting plate; 14. Guide rod; 15. Filter screen; 16. Blinding plate; 17. Sealing plate; 18. Filter box; 19. Positioning frame; 20. Ultrafiltration membrane; 21. Power motor; 22. Transmission disc; 23. Limiting protrusion; 24. Guide block; 25. Resetter; 26. Transmission rod; 27. Guide rod; 28. Airbag; 29. ​​Connecting pipe; 30. Conduit; 31. Hose; 32. Dust cover; 33. Guide frame. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Please see Figure 1-9This invention provides a technical solution: an antibody extraction and purification device, comprising a main body 1 and a stirring tank 2 mounted on the upper end. A feed pipe 3 is connected to the upper left side of the main body 1. The lower end of the stirring tank 2 is funnel-shaped and fixed to the inner wall of the main body 1. The ends of stirring plates 6 are in contact with the inner wall of the stirring tank 2, and the stirring plates 6 are arranged in an array around the drive shaft 5. The stirring tank 2 is fixed to the inner wall of the main body 1, and a stirring motor 4 is fixed to the upper end of the stirring tank 2. The lower shaft end of the stirring motor 4 is connected to the drive shaft 5, and the outer wall of the drive shaft 5 is connected to the stirring plates 6. The lower end of the drive shaft 5 is connected to an auger shaft 7, which is located on the lower side of the stirring tank 2 and is adapted to the lower inner wall of the stirring tank 2. During use, the purification device is placed stably on the ground, and the antibody raw material is transported to the stirring tank 2 at the top of the main body 1 through the feed pipe 3. When the raw material is transported into the stirring tank 2, the stirring motor 4 drives the drive shaft 5 to rotate. When the drive shaft 5 rotates, it drives the stirring plate 6 to rotate synchronously, realizing the stirring and dispersion of the raw material, providing a good foundation for subsequent filtration and purification, and improving the filtration and purification efficiency. At the same time, the drive shaft 5 can synchronously drive the auger shaft 7 to rotate when rotating, and after stirring is completed, the stirring motor 4 can be controlled to rotate in reverse. The reverse rotation of the auger shaft 7 is used to quickly discharge the raw material, and the funnel-shaped stirring tank 2 can also reduce the residue of raw material and improve the overall operation efficiency.

[0029] A one-way rotating rod 8 is rotatably connected inside the feed pipe 3, and a limiting plate 9 is fixed to the outer wall of the one-way rotating rod 8. The limiting plate 9 is adapted to the inner wall of the feed pipe 3. The right side of the one-way rotating rod 8 extends outside the feed pipe 3, and a driven bevel gear 10 is fixed to the right end of the one-way rotating rod 8. The lower end of the stirring motor 4 is connected to a driving bevel gear 11, and the driving bevel gear 11 meshes with the driven bevel gear 10. When the stirring motor 4 rotates, it drives the driving bevel gear 11 to rotate synchronously, and the driving bevel gear 11 drives the driven bevel gear 10 and the one-way rotating rod 8. Rotating rod 8 drives multiple sets of limiting plates 9 to rotate synchronously, achieving uniform and quantitative feeding of antibody raw materials. Simultaneous stirring can be performed during feeding. After feeding is completed, the stirring motor 4 is controlled to rotate in the opposite direction. The unidirectional rotating rod 8 has an internal ratchet structure, enabling unidirectional transmission. When the stirring motor 4 rotates in the opposite direction, the feeding structure stops rotating. During reverse rotation, the drive shaft 5 and the auger shaft 7 rotate in the opposite direction. The auger shaft 7, in conjunction with the lower end of the stirring tank 2, enables rapid discharge of raw materials, improving the efficiency of subsequent filtration. A filter hopper 12 is installed at the lower end of the mixing tank 2. A limiting plate 13 is fixed at the upper end of the filter hopper 12, and a filter screen 15 is connected inside the filter hopper 12. A power motor 21 is fixed on both sides of the main body 1, and a transmission disc 22 is fixed on the inner side of the power motor 21. A transmission rod 26 is fixed on the inner side of the transmission disc 22. Guide frames 33 are fixed on both sides of the filter hopper 12. A limiting plate 13 is fixed at the upper end of the filter hopper 12. A through hole is opened in the middle of the limiting plate 13, and the lower end of the mixing tank 2 extends into the through hole. Guide rods 14 pass through both sides of the filter hopper 12. The guide rod 14 is slidably connected to the filter hopper 12, and both ends of the guide rod 14 are fixed to the inner wall of the main body 1 of the device. The transmission rod 26 is connected to the edge of the transmission disc 22. The guide frame 33 is rectangular, and the transmission rod 26 is slidably connected to the inner wall of the guide frame 33. After the mixing tank 2 has finished mixing the material, it is discharged into the filter hopper 12. The filter screen 15 fixed in the filter hopper 12 is used to intercept and filter debris and larger impurities. The pre-filtration of the filter hopper 12 can remove large particles, microorganisms, fine particles, and residual cells. To improve the filtration efficiency of impurities, the reciprocating motion of the filter hopper 12 is driven by the power motor 21 to improve the filtration efficiency. The power motor 21 drives the transmission disc 22 to rotate, and when the transmission disc 22 rotates, it can drive the transmission rod 26 to make a circular motion. When the transmission rod 26 rotates, it slides inside the guide frame 33 and drives the filter hopper 12 to reciprocate to improve the filtration efficiency. When the filter hopper 12 reciprocates, the guide rod 14 can be used to improve its activity stability and reliability.

[0030] A filter box 18 is provided at the lower end of the filter hopper 12. A positioning frame 19 is provided inside the filter box 18, and an ultrafiltration membrane 20 is installed inside the positioning frame 19. Guide blocks 24 are fixed at the upper ends of both sides of the positioning frame 19. Limiting protrusions 23 are connected to the outer wall of the transmission disk 22. The transmission disk 22 passes through the lower end of the baffle 16. The positioning frame 19 is slidably connected to the inner wall of the filter box 18. Resetters 25 are connected to the lower ends of both sides of the positioning frame 19. The lower ends of the resetters 25 are fixed to the inner wall of the filter box 18. Guide blocks 24 are fixed at the upper ends of both sides of the positioning frame 19. Limiting protrusions 23 are connected to the outer wall of the transmission disk 22. The limiting protrusions 23 are arranged in a ring array around the center of the transmission disk 22. The outer wall of the limiting protrusions 23 contacts the upper end of the guide blocks 24. After initial filtration in the filter bucket 12, the raw material is conveyed to the filter box 18, where it undergoes further filtration using the ultrafiltration membrane 20 installed inside the filter box 18. Ultrafiltration removes molecular impurities (such as HCP, DNA, viruses, and aggregates) and some small molecule contaminants. As the drive disc 22 rotates, it drives the limiting protrusion 23 to rotate synchronously. The rotating limiting protrusion 23 continuously presses the guide block 24 downwards, and the guide block 24 pushes the positioning frame 19 and the ultrafiltration membrane 20 downwards. Meanwhile, the reset device 25 pushes the positioning frame 19 and the ultrafiltration membrane 20 upwards, thereby causing the drive disc 22 to drive the ultrafiltration membrane 20 to bounce up and down, improving filtration efficiency and reducing the probability of clogging. The antibody purity after ultrafiltration typically reaches 90-95%. Combined with steps such as chromatography and virus filtration, the final product purity is ≥98%.

[0031] A baffle plate 16 is provided at the lower end of the filter bucket 12, and the lower end of the filter bucket 12 passes through the lower end of the baffle plate 16. A through hole is opened in the middle of the baffle plate 16. Sealing plates 17 are fixed on both sides of the filter bucket 12, and the sealing plates 17 pass through the interior of the baffle plate 16 on both sides. The baffle plate 16 is slidably connected to the interior of the through hole. Guide rods 27 are fixed on both sides of the filter bucket 12, and airbags 28 are fixed on the outer side of the guide rods 27. Two sets of airbags 28 are symmetrically arranged at the front and back. A connecting pipe 29 is connected to the middle of the left side of the airbag 28. An air outlet one-way valve is connected to the middle of the connecting pipe 29. A hose 31 is connected to the lower end of the air outlet one-way valve. A conduit 30 is connected to the lower end of the hose 31. The lower end of the conduit 30 passes through the baffle plate 16. The outer end of the airbag 28 passes through the exterior of the main body 1 of the device. Furthermore, an air inlet check valve is connected to the outer end of the air bladder 28, and a dust cover 32 is provided on the outside of the air inlet check valve, and the dust cover 32 is fixed to the outer wall of the device body 1. During the reciprocating motion of the filter bucket 12, the air bladder 28 can be squeezed synchronously by the guide rod 27. When the air bladder 28 is squeezed, the gas inside the air bladder 28 can be transported to the lower end of the sealing plate 17 through the connecting pipe 29, the air outlet check valve, the hose 31 and the conduit 30, thereby increasing the filtration efficiency of the ultrafiltration membrane 20 after pressurization. During the reciprocating motion of the filter bucket 12, when the air bladder 28 is reset, the air inlet check valve can be used to draw in external air, and a dust cover 32 is provided on the outside of the air inlet check valve to prevent impurities from entering the device body 1 and causing antibody contamination.

[0032] An apparatus for antibody extraction and purification includes the following steps: Step 1: The antibody raw material is fed at a uniform rate and in a quantitative manner, and stirring is carried out simultaneously during the feeding process. Step 2: After the antibody is fed into the container, continue stirring to disperse it. After stirring, further feed the antibody raw materials. Step 3: After stirring and dispersing, the raw materials are pre-filtered to remove larger debris and impurities; Step 4: After removing some impurities, use an ultrafiltration structure for final filtration and store the filtered and purified antibody raw material.

[0033] The contents not described in detail in this specification are prior art known to those skilled in the art. Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An apparatus for extracting and purifying antibodies, comprising a main body (1) and a stirring tank (2) mounted on the upper end, wherein a feed pipe (3) is connected to the upper left side of the main body (1), a filter bucket (12) is provided at the lower end of the stirring tank (2), and a filter box (18) is provided at the lower end of the filter bucket (12), characterized in that: The mixing tank (2) is fixed to the inner wall of the main body (1) of the device, and the mixing motor (4) is fixed at the upper end of the mixing tank (2). The lower shaft end of the mixing motor (4) is connected to the transmission shaft (5), and the outer wall of the transmission shaft (5) is connected to the mixing plate (6). The upper end of the filter bucket (12) is fixed with a limiting plate (13), and a filter screen (15) is connected inside the filter bucket (12). The main body (1) of the device is fixed with a power motor (21) on both sides, and a transmission disk (22) is fixed on the inner end of the power motor (21), and a transmission rod (26) is fixed on the inner side of the transmission disk (22). The filter bucket (12) is fixed with guide frames (33) on both sides. The filter box (18) is provided with a positioning frame (19) and an ultrafiltration membrane (20) is installed inside the positioning frame (19). Guide blocks (24) are fixed on the upper ends of both sides of the positioning frame (19), and limit protrusions (23) are connected to the outer wall of the transmission disk (22).

2. The apparatus for antibody extraction and purification according to claim 1, characterized in that: The lower end of the mixing tank (2) is designed in the shape of a funnel, and the mixing tank (2) is fixed to the inner wall of the main body (1) of the device. The end of the mixing plate (6) is in contact with the inner wall of the mixing tank (2), and the mixing plate (6) is arranged in an array with the drive shaft (5) as the axis.

3. The apparatus for antibody extraction and purification according to claim 2, characterized in that: The lower end of the drive shaft (5) is connected to the auger shaft (7), and the auger shaft (7) is located on the lower side of the mixing tank (2), and the auger shaft (7) is adapted to the inner wall of the lower end of the mixing tank (2).

4. The apparatus for antibody extraction and purification according to claim 1, characterized in that: The feed pipe (3) is rotatably connected to a one-way rotating rod (8), and a limiting plate (9) is fixed on the outer wall of the one-way rotating rod (8). The limiting plate (9) is adapted to the inner wall of the feed pipe (3). The one-way rotating rod (8) extends to the outside of the feed pipe (3) on the right side, and a driven bevel gear (10) is fixed at the right end of the one-way rotating rod (8). The lower end of the stirring motor (4) is connected to a driving bevel gear (11), and the driving bevel gear (11) meshes with the driven bevel gear (10).

5. The apparatus for antibody extraction and purification according to claim 1, characterized in that: The upper end of the filter bucket (12) is fixed with a limiting plate (13), and a through hole is opened in the middle of the limiting plate (13). The lower end of the stirring tank (2) extends into the through hole. Guide rods (14) pass through both sides of the filter bucket (12), and the guide rods (14) are slidably connected to the filter bucket (12). The two ends of the guide rods (14) are fixed to the inner wall of the main body (1) of the device.

6. The apparatus for antibody extraction and purification according to claim 1, characterized in that: The transmission rod (26) is connected to the edge of the transmission disk (22), the guide frame (33) is a rectangular frame design, and the transmission rod (26) is slidably connected to the inner wall of the guide frame (33).

7. The apparatus for antibody extraction and purification according to claim 6, characterized in that: The filter bucket (12) is provided with a baffle plate (16) at its lower end, and the lower end of the filter bucket (12) passes through the lower end of the baffle plate (16). A through hole is opened in the middle of the baffle plate (16). Sealing plates (17) are fixed on both sides of the filter bucket (12), and the sealing plates (17) pass through the interior of the baffle plate (16) on both sides. The baffle plate (16) is slidably connected to the interior of the through hole, and the transmission disc (22) passes through the lower end of the baffle plate (16).

8. The apparatus for antibody extraction and purification according to claim 1, characterized in that: The positioning frame (19) is slidably connected to the inner wall of the filter box (18), and a resetter (25) is connected to the lower ends of both sides of the positioning frame (19). The lower end of the resetter (25) is fixed to the inner wall of the filter box (18). A guide block (24) is fixed to the upper ends of both sides of the positioning frame (19). A limiting protrusion (23) is connected to the outer wall of the transmission disk (22). The limiting protrusion (23) is arranged in a ring array with the center of the transmission disk (22) as the axis. The outer wall of the limiting protrusion (23) is in contact with the upper end of the guide block (24).

9. The apparatus for antibody extraction and purification according to claim 8, characterized in that: The filter bucket (12) has guide rods (27) fixed on both sides, and airbags (28) are fixed on the outside of the guide rods (27). Two sets of airbags (28) are symmetrically arranged in front and behind, and the middle of the left side of the airbag (28) is connected to a connecting pipe (29). The middle of the connecting pipe (29) is connected to an outlet one-way valve. The lower end of the outlet one-way valve is connected to a hose (31), and the lower end of the hose (31) is connected to a conduit (30). The lower end of the conduit (30) passes through the baffle (16). The outer end of the airbag (28) passes through the outside of the main body (1) of the device, and the outer end of the airbag (28) is connected to an inlet one-way valve. The inlet one-way valve is covered with a dust cover (32), and the dust cover (32) is fixed to the outer wall of the main body (1) of the device.

10. An apparatus for antibody extraction and purification according to any one of claims 1-9, characterized in that: Includes the following steps: Step 1: The antibody raw material is fed at a uniform rate and in a quantitative manner, and stirring is carried out simultaneously during the feeding process. Step 2: After the antibody is fed into the container, continue stirring to disperse it. After stirring, further feed the antibody raw materials. Step 3: After stirring and dispersing, the raw materials are pre-filtered to remove larger debris and impurities; Step 4: After removing some impurities, use an ultrafiltration structure for final filtration and store the filtered and purified antibody raw material.

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