Plant growth promoter

Abstract

A method and raw material for promoting the growth of a plant is provided. A growth promoter for plants selected from the group consisting of fruits and vegetables, leaf and stem vegetables, root vegetables, and cereals, said promoter being obtained by combining (A) ascorbic acid or a salt thereof, (B) an antioxidant, and (C) an organic solvent, the antioxidant (B) being at least one substance selected from the group consisting of tocopherol, dibutylated hydroxytoluene, and butylated hydroxyanisole; the (C) organic solvent is one or more selected from the group consisting of alcohols having 5 or less carbon atoms, dimethyl sulfoxide, and polyhydric alcohols, and when the component (B) is 1, the ratio (mass ratio) of the (A) ascorbic acid or the salt thereof is 10-3,000,000.

Description

Technical Field

[0001] This invention relates to a method and raw materials for promoting plant growth. Background Technology

[0002] Approximately one-third of the Earth's landmass is arid, and this aridity is projected to increase further due to global warming. Furthermore, as a countermeasure to the severe food shortages caused by population growth, the development of technologies to improve, maintain, and increase yields of fruits, vegetables, leafy greens, root vegetables, and grains in arid regions, saline areas, and areas with high or low temperatures—regions where growth is difficult or deteriorates, leading to decreased yields—is of paramount importance.

[0003] Oxygen is essential for the survival of many plants and animals. However, it also produces highly reactive reactive oxygen species (ROS) within plant and animal cells, causing serious damage to organisms, such as gene damage or enzyme inactivation. To address this, plants accumulate large amounts of antioxidants, including L-ascorbic acid, and a complex enzyme system utilizing these antioxidants to scavenge ROS works throughout the cell.

[0004] From this perspective, antioxidants containing ascorbic acid are often incorporated into raw materials applied to plants to promote growth. For example, Non-Patent Document 1 discloses that applying ascorbic acid to olives, sugarcane, wheat, etc., produces growth-promoting and yield-increasing effects. Furthermore, Non-Patent Document 2 discloses that spraying the leaves of leguminous crops with a certain concentration of ascorbic acid dissolved in distilled water three times during their vegetative or reproductive growth stages can achieve a yield increase of up to 30%. Additionally, Patent Document 1 discloses the use of an aqueous composition containing iron(II) compounds and L-ascorbic acid to promote plant growth, and Patent Document 2 discloses the use of an aqueous solution containing glycyrrhizin and L-ascorbic acid to promote plant growth.

[0005] Existing technical documents

[0006] Patent Document 1: Japanese Patent Application Publication No. 60-202805

[0007] Patent Document 2: Japanese Patent Application Publication No. 08-143406

[0008] Non-patent document 1: Akram et al., (2017) Ascorbic Acid-A Potential OxidantScavenger and Its Role in Plant Development and Abiotic Stress Tolerance. Frontiers in Plant Science, 8:613

[0009] Non-patent literature 2: Zarghamnejad et al., (2014) Chickpea response to ascorbicacid foliar application at vegetative and reproductive stages. International Journal of Biosciences, 5: 166-170 Summary of the Invention

[0010] This invention relates to the following 1) to 10).

[0011] 1) A plant growth promoter selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the promoter is a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0012] 2) A plant growth promoter selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the promoter is a combination of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (C) the organic solvent is 4 to 100,000.

[0013] 3) A yield enhancer for plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the enhancer is a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0014] 4) A plant yield enhancer selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the enhancer is a combination of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent, wherein the (B) antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and the (C) organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of the (C) organic solvent is 4 to 100,000.

[0015] 5) A method for promoting the growth of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the method includes: applying a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent with water to soil or plants, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0016] 6) A method for increasing the yield of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the method includes: applying a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent with water to soil or plants, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0017] 7) Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a plant growth promoter selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols having 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0018] 8) Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a plant growth promoter selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols having 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (C) the organic solvent is 4 to 100,000.

[0019] 9) Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a yield enhancer for plants selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols having 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0020] 10) Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a yield enhancer for plants selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols having 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of the organic solvent (C) is 4 to 100,000. Attached Figure Description

[0021] Figure 1 This refers to the fresh weight of the above-ground parts of the cabbage 8 days after foliar spraying.

[0022] Figure 2 This is the dry weight of the above-ground part of the Komatsuna plant 7 days after foliar spraying.

[0023] Figure 3 This is the fresh weight of the above-ground parts of spinach 7 days after foliar spraying.

[0024] Figure 4 This is the dry weight of the above-ground parts of the strawberry plant 22 days after foliar spraying.

[0025] Figure 5 This is the fresh weight of the above-ground part of the lettuce 7 days after foliar spraying.

[0026] Figure 6 This is the fresh weight of the above-ground part of the scallion 7 days after foliar spraying.

[0027] Figure 7 This is the fresh weight of the above-ground part of the scallion 7 days after foliar spraying.

[0028] Figure 8 This is the fresh weight of the underground part of the carrot 7 days after foliar spraying.

[0029] Figure 9 This is the potato yield 12 days after foliar spraying.

[0030] Figure 10 It is the fresh weight of each tomato fruit after foliar spraying treatment.

[0031] Figure 11 It is the fresh weight of each tomato fruit after foliar spraying treatment.

[0032] Figure 12 This refers to the number of fruits per cucumber plant after foliar spraying treatment.

[0033] Figure 13 It is the dry weight of the above-ground parts of the rice plant 7 days after foliar spraying.

[0034] Figure 14 It is the dry weight of the aboveground part of wheat 7 days after foliar spraying.

[0035] Figure 15 This is the dry weight of the above-ground parts of the chives 5 days after foliar spraying.

[0036] Figure 16 This is the fresh weight of the underground part of the radish 7 days after foliar spraying.

[0037] Figure 17 This is the dry weight of the above-ground parts of *Salad Burnet* 7 days after foliar spraying.

[0038] Figure 18 This is the dried weight of the underground part of the bitter gourd 7 days after foliar spraying.

[0039] Figure 19 This is the fresh weight of the above-ground parts of the perilla plant 7 days after foliar spraying.

[0040] Figure 20 This is the fresh weight of the above-ground parts of the celery stalk 7 days after foliar spraying.

[0041] Figure 21 This is the fresh weight of the underground part of the burdock 7 days after foliar spraying.

[0042] Figure 22 This is the fresh weight of the above-ground parts of the beet 7 days after foliar spraying.

[0043] Figure 23 This is the total number of wild strawberry flowers up to 62 days after foliar spraying.

[0044] Figure 24 This is the weight of wheat grains 47 days after foliar spraying.

[0045] Figure 25 This is the fresh weight of the above-ground part of Komatsuna 7 days after foliar spraying.

[0046] Figure 26 This is the fresh weight of the above-ground part of Komatsuna 7 days after foliar spraying.

[0047] Figure 27 This is the fresh weight of the above-ground part of Komatsuna 7 days after foliar spraying.

[0048] Figure 28 This is the fresh weight of the above-ground part of Komatsuna 7 days after foliar spraying.

[0049] Figure 29 This is the fresh weight of the above-ground part of Komatsuna 7 days after foliar spraying.

[0050] Figure 30 This is the corn yield 20 days after foliar spraying.

[0051] Figure 31 This is the fresh weight of the above-ground parts of the melon 7 days after foliar spraying.

[0052] Figure 32 This is the fresh weight of the above-ground parts of the okra 7 days after foliar spraying.

[0053] Figure 33 This is the fresh weight of the above-ground parts of the garland chrysanthemum 7 days after foliar spraying.

[0054] Figure 34 This is the fresh weight of the above-ground part of the pumpkin 6 days after foliar spraying.

[0055] Figure 35 This is the fresh weight of the underground part of a small radish 6 days after foliar spraying. Detailed Implementation

[0056] This invention relates to a method and raw materials for promoting plant growth.

[0057] The inventors conducted research on the growth promotion of plants using ascorbic acid, and found that by combining ascorbic acid with specific antioxidants dissolved in organic solvents, the growth-promoting effect can be achieved in fruits and vegetables, leafy and stem vegetables, root vegetables, or grains.

[0058] According to the method of the present invention, the growth of plants selected from fruits and vegetables, leafy vegetables, root vegetables, and cereals can be increased. That is, according to the present invention, the yield of crops such as fruits and vegetables, leafy vegetables, root vegetables, and cereals can be increased.

[0059] In the plant growth promoter of the present invention, "growth promoter" means to increase the growth (fresh weight, elongation, etc.) of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables, and cereals, thereby increasing yield. When the plant growth promoter of the present invention is specifically intended to increase grain yield, it is referred to as a "plant yield enhancer".

[0060] In this invention, the plants are selected from fruits and vegetables, leafy and stem vegetables, root vegetables, and grains. Examples of fruits and vegetables include cucumber (Cucurbitaceae), watermelon (Cucurbitaceae), cantaloupe (Cucurbitaceae), pumpkin (Cucurbitaceae), zucchini (Cucurbitaceae), bitter melon (Cucurbitaceae), tomato (Solanaceae), eggplant (Solanaceae), green pepper (Solanaceae), strawberry (Rosaceae), wild strawberry (Rosaceae), okra (Malvaaceae), and other fruits and vegetables other than legumes. Examples of leafy and stem vegetables include: cabbage (Brassicaceae), Chinese cabbage (Brassicaceae), bok choy (Brassicaceae), komatsuna (Brassicaceae), cauliflower (Brassicaceae), broccoli (Brassicaceae), lettuce (Asteraceae), garland chrysanthemum (Asteraceae), spinach (Amaranthaceae), celery (Apiaceae), coriander (Apiaceae), scallion (Amaryllidaceae), onion (Amaryllidaceae), leek (Amaryllidaceae), burnet root (Rosaceae), perilla (Perillaaceae), and long-capsulated jute (Malvaaceae). Examples of root vegetables include: potato (Solanaceae), carrot (Apiaceae), white radish (Brassicaceae), small radish (Brassicaceae), turnip (Brassicaceae), burdock (Asteraceae), sugar radish (Amaranthaceae), beet (Amaranthaceae), sweet potato (Convolvulaceae), taro (Araceae), and yam (Dioscoreaceae). Examples of cereals (grains of the Poaceae family) include: rice, wheat, barley, rye, and corn.

[0061] From the perspective of promoting growth, the preferred fruits and vegetables are those belonging to the Cucurbitaceae, Solanaceae, Rosaceae, Malvaceae, or Poaceae families; the preferred leaf and stem vegetables are those belonging to the Brassicaceae, Asteraceae, Amaranthaceae, Apiaceae, Amaryllidaceae, Rosaceae, or Perillaaceae families; and the preferred root vegetables are those belonging to the Solanaceae, Apiaceae, Brassicaceae, Asteraceae, Amaranthaceae, or Convolvulaceae families.

[0062] In addition, among fruits and vegetables, cucumber (Cucurbitaceae), bitter melon (Cucurbitaceae), cantaloupe (Cucurbitaceae), pumpkin (Cucurbitaceae), tomato (Solanaceae), strawberry (Rosaceae), wild strawberry (Rosaceae), and okra (Malvaceae) are preferred. Among leafy and stem vegetables, cabbage (Brassicaceae), komatsuna (Brassicaceae), lettuce (Asteraceae), garland chrysanthemum (Asteraceae), spinach (Chenpoirae), celery (Apiaceae), scallion (Amaryllidaceae), leek (Amaryllidaceae), burnet root (Rosaceae), and perilla (Perillaceae) are preferred. Among root vegetables, potato (Solanaceae), carrot (Apiaceae), radish (Brassicaceae), burdock (Asteraceae), and beet (Amaranthaceae) are preferred. Among grains, rice, wheat, and corn are preferred.

[0063] The plant growth promoter of the present invention is a combination of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent.

[0064] The combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent is carried out by pre-mixing or mixing the components (A), (B), and (C) in appropriate mass ratios. One approach is to combine the components (A), (B), and (C) to form a single formulation (composition). Another approach is to prepare, for example, a two-dosage formulation (set) containing component (A) and a formulation containing components (B) and (C) respectively, which are then combined upon use.

[0065] In this invention, the IUPAC system name for ascorbic acid, which is component (A), is (R)-3,4-dihydroxy-5-((S)-1,2-dihydroxyethyl)furan-2(5H)-one. Ascorbic acid can be any of the D-type, L-type, and DL-type, preferably the L-type (so-called L-ascorbic acid).

[0066] Ascorbic acid can be used in various grades of commercially available products.

[0067] Salts of ascorbic acid include, for example, sodium salts, potassium salts, calcium salts, magnesium salts, barium salts, ammonium salts, and salts of nitrogen-containing organic bases such as pyridine, trimethylamine, triethylamine, tributylamine, and diethylamine.

[0068] When the plant growth promoter of the present invention is applied to plants, the concentration of (A) ascorbic acid or its salt in the composition may, for example, be in the range of more than 100 ppm by mass and less than 300,000 ppm by mass, and may be appropriately adjusted according to the method of supply.

[0069] For example, when spraying using a sprayer (e.g., a nozzle sprayer), the concentration of the spray solution is preferably 100 ppm by mass or more, more preferably 300 ppm by mass or more, even more preferably 500 ppm by mass or more, and preferably 20,000 ppm by mass or less, more preferably 10,000 ppm by mass or less, and even more preferably 4,500 ppm by mass or less. Furthermore, it is preferably 100 to 20,000 ppm by mass, more preferably 300 to 10,000 ppm by mass, and even more preferably 500 to 4,500 ppm by mass.

[0070] Furthermore, when spraying in the air, the concentration of the spray liquid is preferably 20,000 ppm by mass or more, more preferably 80,000 ppm by mass or more, even more preferably 150,000 ppm by mass or more, and preferably 300,000 ppm by mass or less, more preferably 250,000 ppm by mass or less, and even more preferably 200,000 ppm by mass or less. Additionally, the concentration is preferably 20,000 to 300,000 ppm by mass, more preferably 80,000 to 250,000 ppm by mass, and even more preferably 150,000 to 200,000 ppm by mass.

[0071] In this invention, the antioxidant as component (B) is specifically selected from at least one of tocopherol (vitamin E), butylated hydroxytoluene (BHT), and butylated hydroxyanisole (BHA). Among them, BHT and BHA are preferred as phenolic antioxidants, and BHT is more preferred.

[0072] Furthermore, in this invention, as an antioxidant, antioxidants other than tocopherol, BHT and BHA may also be used in combination, such as sodium isoascorbate, propyl gallate, sodium sulfite, potassium sulfite, potassium metabisulfite, chlorogenic acid, catechin, glutathione, uric acid, etc., preferably one or more of sodium sulfite, potassium sulfite, potassium metabisulfite, chlorogenic acid, catechin, glutathione and uric acid.

[0073] Therefore, as an antioxidant for component (B), preferably, one or more phenolic antioxidants selected from BHT and BHA may be used, and in addition to the phenolic antioxidant, one or more of sodium sulfite, potassium sulfite, glutathione and uric acid may be used in combination.

[0074] When ascorbic acid is dissolved in tap water or other water containing metal ions, the metal ions react with the ascorbic acid to produce hydrogen peroxide. The hydrogen peroxide further reacts with the metal ions in a Fenton reaction to generate hydroxyl radicals. If these hydroxyl radicals invade the plant, they trigger a continuous lipid peroxidation reaction, reacting with lipids present in cell membranes and other structures to generate lipid free radicals or lipid peroxide free radicals. These free radicals can be captured by using antioxidants.

[0075] In this invention, (B) the antioxidant is used in a solution dissolved in (C) the organic solvent, thereby effectively exerting the plant growth promoting effect of ascorbic acid.

[0076] Therefore, in the plant growth promoter of the present invention, it is preferable to prepare a two-component formulation (set) by separately preparing a formulation containing component (A) (first agent) and a formulation containing components (B) and (C) (second agent), and use the two in a mixed manner. By preparing a two-component formulation, discoloration that occurs when storing the formulation containing all components (A), (B), and (C) can be suppressed.

[0077] In this case, the first agent containing ingredient (A) may contain an antioxidant different from tocopherol, BHT and BHA, such as one or more selected from sodium sulfite, potassium sulfite, potassium metabisulfite, chlorogenic acid, catechin, glutathione and uric acid.

[0078] The concentration of antioxidant (B) in the composition when the plant growth promoter of the present invention is applied to plants is preferably 0.001 ppm by mass or more, more preferably 0.01 ppm by mass or more, even more preferably 0.1 ppm by mass or more, and preferably 100 ppm by mass or less, more preferably 20 ppm by mass or less, and even more preferably 5 ppm by mass or less. Furthermore, it is preferably 0.001 to 100 ppm by mass, more preferably 0.01 to 20 ppm by mass, and even more preferably 0.1 to 5 ppm by mass.

[0079] The organic solvent used as component (C) is a solvent for dissolving the antioxidant (B), specifically selected from one or more alcohols having five or fewer carbon atoms, dimethyl sulfoxide (DMSO), and polyols.

[0080] Here, alcohols with 5 or fewer carbon atoms are preferably listed as ethanol, methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, 2-methyl-2-propanol, isobutanol, 1-pentanol, 3-methyl-1-butanol, 2,2-dimethyl-1-propanol, etc.

[0081] Examples of polyols include: diols such as ethylene glycol, diethylene glycol, hexanediol, polyethylene glycol, propylene glycol, isopentyl glycol, 1,3-propanediol, dipropylene glycol, and polypropylene glycol; triols or more such as glycerol, diglycerol, and triglycerol; and sugars or sugar alcohols such as erythritol, pentaerythritol, maltitol, xylitol, sorbitan, and sorbitol. Ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, and glycerol are preferred, and diethylene glycol, propylene glycol, and glycerol are more preferred.

[0082] The organic solvents used in (C) are more preferably isobutanol, DMSO and glycerol.

[0083] When using one or more of BHT and BHA as an antioxidant (B), DMSO, isobutanol, or a mixture thereof is preferably used as an organic solvent (C).

[0084] When the plant growth promoter of the present invention is applied to plants, the concentration of (C) organic solvent in the composition can be appropriately adjusted within the range of more than 1 ppm by mass and less than 100,000 ppm by mass, depending on the method of supply.

[0085] For example, when spraying using a sprayer (e.g., a nozzle sprayer), the concentration of the spray solution is preferably 1 ppm or more by mass, more preferably 10 ppm or more by mass, even more preferably 50 ppm or more by mass, and preferably 10,000 ppm or less by mass, more preferably 5,000 ppm or less by mass, and even more preferably 1,000 ppm or less by mass. Furthermore, it is preferably 1 to 10,000 ppm by mass, more preferably 10 to 5,000 ppm by mass, and even more preferably 50 to 1,000 ppm by mass.

[0086] Furthermore, in the case of aerial spraying, the concentration in the spray liquid is preferably 100 ppm or more by mass, more preferably 1,000 ppm or more by mass, even more preferably 5,000 ppm or more by mass, and preferably 100,000 ppm or less by mass, more preferably 50,000 ppm or less by mass, and even more preferably 10,000 ppm or less by mass. Additionally, it is preferably 100 to 100,000 ppm by mass, more preferably 1,000 to 50,000 ppm by mass, and even more preferably 5,000 to 10,000 ppm by mass.

[0087] The ratio (by mass) of (A) ascorbic acid or its salt, (B) antioxidant, and (C) organic solvent is such that when component (B) is set to 1, component (A) is preferably 10 or more, more preferably 100 or more, further preferably 500 or more, and preferably 3,000,000 or less, more preferably 2,000,000 or less, further preferably 200,000 or less. Additionally, it is preferably 10 to 3,000,000, more preferably 100 to 2,000,000, and further preferably 500 to 200,000. When spraying with a sprayer, the concentration is preferably 500 to 45,000; when spraying by aerial spraying, the concentration is preferably 150,000 to 2,000,000; more preferably 500 to 4,500 when spraying with a sprayer; and preferably 150,000 to 200,000 when spraying by aerial spraying.

[0088] Furthermore, when component (B) is set to 1, component (C) is preferably 4 or more, more preferably 10 or more, further preferably 20 or more, further preferably 50 or more, and preferably 100,000 or less, more preferably 50,000 or less, further preferably 20,000 or less, and further preferably 10,000 or less. Additionally, it is preferably 4 to 100,000, more preferably 10 to 50,000, further preferably 20 to 20,000, and further preferably 50 to 10,000. Specifically, when spraying using a sprayer, it is preferably 20 to 10,000, and when spraying by aerial spraying, it is preferably 2,000 to 100,000. Furthermore, when spraying using a sprayer, it is more preferably 50 to 1,000, and when spraying by aerial spraying, it is more preferably 5,000 to 10,000.

[0089] In this invention, (D) surfactants can be used in combination with (A) ascorbic acid or its salts, (B) antioxidants and (C) organic solvents.

[0090] By using surfactants, the wettability, adhesion, and penetration of (A) ascorbic acid or its salts on plant surfaces can be improved, thereby enhancing the effect of (A) ascorbic acid or its salts or making it more effective.

[0091] As described above, when the plant growth promoter of the present invention is formulated into a two-component formulation (set) comprising a formulation (first agent) containing component (A) and a formulation (second agent) containing components (B) and (C), the surfactant (D) is preferably contained in the second agent containing components (B) and (C).

[0092] As surfactants, examples include nonionic surfactants, anionic surfactants, and amphoteric surfactants. One or more of these can be used, and preferably, at least one nonionic surfactant is contained.

[0093] As nonionic surfactants, examples include one or more selected from sorbitan fatty acid esters, glycerol fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, polyglycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters, polyoxyethylene alkyl ethers, polyoxyethylene alkyl aryl ethers, polyoxyethylene aryl ethers, polyoxyethylene alkenyl ethers, alkyl polysaccharide glycosides, polyoxyethylene alkyl polysaccharide glycosides, and sucrose fatty acid esters.

[0094] As anionic surfactants, examples include one or more selected from alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene alkenyl ether sulfates and polyoxyethylene alkyl aryl ether sulfates, fatty acid salts, pyrophosphates, lauryl phosphates, polycarboxylic acid polymers, polyoxyethylene alkylene alkylacetic acids, aromatic sulfonate formaldehyde condensates, polyoxyethylene styrene ether sulfates, alkyl diphenyl ether disulfonates, dialkyl sulfosuccinates, alkyl naphthalene sulfonates, etc.

[0095] As amphoteric surfactant, one or more can be selected from, for example, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazoline betaine, alkyl dimethylaminoacetic acid betaine, alkyl acid amide propyl betaine, etc.

[0096] From the viewpoint of preventing phytotoxicity to plants due to over-application, the nonionic surfactant is preferably selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters, polyoxyethylene alkyl ethers, alkyl polysaccharides, and sucrose fatty acid esters; more preferably, it is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters, and polyoxyethylene alkyl ethers. The anionic surfactant is preferably selected from one or more of alkyl sulfates (e.g., sodium lauryl sulfate, ammonium lauryl sulfate, triethanolamine lauryl sulfate, etc.), aromatic sulfonate formaldehyde condensates, and fatty acid salts. The amphoteric surfactant is preferably 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazoline betaine.

[0097] When the plant growth promoter of the present invention is applied to plants, the concentration of the surfactant (D) in the composition can be in the range of 10 ppm by mass to 30,000 ppm by mass, and can be appropriately adjusted according to the method of supply.

[0098] For example, when spraying using a sprayer (e.g., a nozzle sprayer), the concentration of the spray solution is preferably 10 ppm by mass or more, more preferably 100 ppm by mass or more, even more preferably 200 ppm by mass or more, and preferably 5,000 ppm by mass or less, more preferably 1,000 ppm by mass or less, and even more preferably 500 ppm by mass or less. Furthermore, a concentration of 10 to 5,000 ppm by mass is preferred, more preferably 10 to 1,000 ppm by mass, and even more preferably 100 to 500 ppm by mass.

[0099] Furthermore, when spraying in the air, the concentration of the spray solution is preferably 100 ppm or more by mass, more preferably 500 ppm or more by mass, even more preferably 1,000 ppm or more by mass, and preferably 10,000 ppm or less by mass, more preferably 8,000 ppm or less by mass, and even more preferably 5,000 ppm or less by mass. Additionally, it is preferably 100 to 10,000 ppm by mass, more preferably 500 to 8,000 ppm by mass, and even more preferably 1,000 to 5,000 ppm by mass.

[0100] Furthermore, when the proportion (mass ratio) of surfactant (D) in combination is set to 1, component (B) is preferably 10 or more, more preferably 50 or more, and even more preferably 100 or more, and preferably 300,000 or less, more preferably 100,000 or less, and even more preferably 5,000 or less. Additionally, it is preferably 10 to 300,000, more preferably 50 to 100,000, and even more preferably 100 to 5,000. Specifically, when spraying using a sprayer, it is preferably 100 to 5,000, and when spraying by aerial spraying, it is preferably 1,000 to 50,000. Furthermore, when spraying using a sprayer, it is more preferably 100 to 500, and when spraying by aerial spraying, it is even more preferably 1,000 to 5,000.

[0101] In addition, in this invention, the chelating agent (E) can be used in combination with (A) ascorbic acid or its salt, (B) antioxidants and (C) organic solvents.

[0102] By using chelating agents, the stability of (A) ascorbic acid or its salts can be improved, thereby stabilizing the effects of ascorbic acid or its salts.

[0103] As described above, when the plant growth promoter of the present invention is made into a two-component formulation (set) consisting of a formulation containing component (A) (first agent) and a formulation containing components (B) and (C) (second agent), it is preferable that the chelating agent (E) is included in the first agent containing component (A).

[0104] Examples of chelating agents include aminocarboxylic acid chelating agents, phosphonic acid chelating agents, hydroxycarboxylic acid chelating agents, and polycarboxylic acid chelating agents. Examples of aminocarboxylic acid chelating agents include: ethylenediaminetetraacetic acid (EDTA), nitrotriacetic acid (NTA), hydroxyethylethylenediaminetriacetic acid (HEDTA), diethylenetriaminepentaacetic acid (DTPA), methylglycine diacetic acid (MGDA), triethylenetetraminehexaacetic acid (TTHA), glutamic acid diacetic acid (GLDA), hydroxyethyliminodiacetic acid (HIDA), dihydroxyethylglycine (DHEG), aspartic acid diacetic acid (ASDA), ethylenediaminesuccinic acid (EDDS), and their salts.

[0105] Examples of phosphonic acid chelating agents include: hydroxyethylidene diphosphonic acid (HEDP), nitrotrimethylenephosphonic acid (NTMP), phosphonobutanetricarboxylic acid (PBTC), ethylenediaminetetramethylenephosphonic acid (EDTMP), and their salts.

[0106] Examples of hydroxycarboxylic acid chelating agents include citric acid, malic acid, tartaric acid, gluconic acid, lactic acid, and their salts.

[0107] Examples of polycarboxylic acid chelating agents include: succinic acid, oxalic acid, glutaric acid, adipic acid, fumaric acid, malonic acid, and their salts.

[0108] The concentration of the (E) chelating agent in the composition when the plant growth promoter of the present invention is applied to plants is preferably 0.01 ppm by mass or more, more preferably 0.1 ppm by mass or more, further preferably 1 ppm by mass or more, and preferably 100 ppm by mass or less, more preferably 50 ppm by mass or less, and further preferably 10 ppm by mass or less. Additionally, it is preferably 0.01 to 100 ppm by mass, more preferably 0.1 to 50 ppm by mass, and further preferably 1 to 10 ppm by mass.

[0109] Furthermore, regarding the proportion (mass ratio) of the chelating agent in the combination (E), when component (B) is set to 1, component (E) is preferably 0.01 or more, more preferably 0.1 or more, and even more preferably 1 or more, and preferably 100 or less, more preferably 50 or less, and even more preferably 10 or less. Additionally, it is preferably 0.01 to 100, more preferably 0.1 to 100, even more preferably 1 to 100, and even more preferably 1 to 10.

[0110] As a preferred combination that can be used with (A) ascorbic acid, (B) antioxidants, (C) organic solvents, and (D) surfactants, the following combinations can be listed as examples.

[0111] • Component (B) contains one or more antioxidants selected from BHT and BHA, component (C) contains an organic solvent containing isobutanol or DMSO, component (D) contains one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters and polyoxyethylene alkyl ethers, and, as required, also contains a combination of one or more surfactants selected from fatty acid salts, aromatic sulfonate formaldehyde condensates and alkyl sulfates.

[0112] • Component (B) is an antioxidant containing BHT, sodium sulfite, glutathione and uric acid; component (C) is an organic solvent containing isobutanol or DMSO; and component (D) is a combination of surfactants containing one or more selected from sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters and polyoxyethylene alkyl ethers, and, if required, also containing alkyl sulfates.

[0113] As shown in the examples described later, in the case of cultivating fruits and vegetables, leafy vegetables, root vegetables and grains, the addition of antioxidants such as BHT and organic solvents to ascorbic acid has been shown to promote the growth of the plants.

[0114] Therefore, ascorbic acid, in combination with specific antioxidants and organic solvents, can be used as a growth promoter for plants selected from fruits, vegetables, leafy greens, root vegetables, and cereals, and can be used to promote the growth of these plants. In addition, it can be used to manufacture growth promoters for plants selected from fruits, vegetables, leafy greens, root vegetables, and cereals.

[0115] The aforementioned plant growth promoters can be compositions (e.g., various agricultural or horticultural materials) used to promote the growth of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, or raw materials (monomers) or preparations used to add to or combine with cultivation substrates for cultivating plants, such as soil, culture medium, and hydroponics solutions.

[0116] The above composition can be in the form of a liquid or gel, or in a solid state (block, powder, granules, etc.).

[0117] Here, the composition can be a composition in which components (A), (B) and (C) are mixed in advance, or it can be a composition in which a preparation containing component (A) and a preparation containing components (B) and (C) are prepared separately and then mixed together when used.

[0118] The above composition may contain, in addition to the components (A) to (C) used in this invention, and further components (D) and (E), any other components. Examples of such components include: solvents (e.g., water, buffer solutions, culture media, hydroponics solutions, etc.), carriers (diatomaceous earth or vermiculite, perlite, peat moss, activated carbon, humus, talc, zeolite, clay, carbon black, pulp, rice straw, soybean residue, bentonite, kaolin, montmorillonite, alumina, etc.), pH adjusters to promote the dissolution of the above compounds, spreading agents to improve spreading power on plants or soil, fertilizer components to enhance fertilizer efficiency, pesticide components, binders, expanders, rhizobia or fungi. Plant growth promoting microorganisms such as root fungi, essential plant nutrients, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleic acid bases, sugars, monohydric alcohols, food additives, microbial extracts, plant hormones, NOD factors (i.e., lipid chitosan oligosaccharides), synthetic lipid chitosan oligosaccharides, chitosan oligosaccharides, chitinous compounds, linoleic acid or its derivatives, linolenic acid or its derivatives, Karrikin, acyl-homoserine lactone derivatives, betaine compounds, phenolic compounds, etc.

[0119] When using the growth promoter of the present invention in the form of a single-dose formulation, from the perspective of the preservation stability of the formulation or the inhibition of browning, it is preferable to combine it with one or more carriers selected from zeolite, silica, bentonite, and mirabilite; from the viewpoint of preservation stability and browning inhibition, silica is more preferably combined. In this case, the concentration of the carrier (component (F)) in the composition is preferably 50 ppm by mass or more, more preferably 100 ppm by mass or more, further preferably 250 ppm by mass or more, and preferably 1,000 ppm by mass or less, more preferably 750 ppm by mass or less, and even more preferably 650 ppm by mass or less. Furthermore, it is preferably 50 to 1,000 ppm by mass, more preferably 100 to 750 ppm by mass, and even more preferably 250 to 650 ppm by mass.

[0120] Furthermore, when the component (B) is set to 1, the proportion (mass ratio) of component (F) is preferably 50 or more, more preferably 100 or more, and even more preferably 250 or more, and preferably 1,000 or less, more preferably 750 or less, and even more preferably 650 or less. Additionally, it is preferably 50 to 1,000, more preferably 100 to 750, and even more preferably 250 to 650.

[0121] In addition, the composition for aerial spraying may contain emulsifiers such as polyoxyethylene fatty acid esters and oils such as decanol.

[0122] Examples of the above-described compositions include, but are not limited to, cultivation substrates (e.g., agricultural or horticultural soil, potting mix, culture medium, hydroponics solution, water, etc.), fertilizers, irrigation water, microbial materials such as rhizobium materials, soil conditioners, pesticides, sowing materials, plant supplements (e.g., activators, nutrients, etc.) containing at least components (A) to (C) of the present invention.

[0123] This fertilizer, microbial material, soil conditioner, sowing material, and plant supplement are preferred because they help improve the soil for cultivating plants. This fertilizer, microbial material, soil conditioner, sowing material, and plant supplement can be solid or liquid; in the case of a solid, it can be in the form of blocks, powder, granules, etc., preferably powder or granules. In addition to containing ingredients (A) to (C) as active ingredients, this fertilizer, microbial material, soil conditioner, sowing material, and plant supplement may also contain ingredients commonly used in fertilizers, microbial materials, soil conditioners, sowing materials, and plant supplements for plant cultivation.

[0124] The microbial materials such as cultivation substrates, fertilizers, rhizobium materials, soil conditioners, pesticides, sowing materials, and plant supplements of this invention can be prepared by adding the components (A) to (C) of this invention to ordinary cultivation substrates (e.g., agricultural or horticultural soil, potting soil, culture medium, hydroponics solution, water, etc.), fertilizers, microbial materials such as rhizobium materials, soil conditioners, pesticides, sowing materials, and plant supplements (e.g., activators, nutrients, etc.).

[0125] It can be a composition in which a formulation containing component (A) and a formulation containing components (B) and (C) are prepared separately, and the two are mixed together when used. In the case of a two-dosage formulation in which a formulation containing component (A) (first agent) and a formulation containing components (B) and (C) (second agent) are prepared and the two are mixed together when used, when the second agent is set to 1, the weight ratio of the first agent is 1 to 100, preferably 1 to 50, more preferably 1 to 25.

[0126] When the first agent and the second agent are prepared by dissolving them separately in water, the concentration of the first agent in the aqueous solution is preferably set to 100 ppm by mass or more and 300,000 ppm by mass or less, and the concentration of the second agent in the aqueous solution is preferably set to 10 ppm by mass or more and 100,000 ppm by mass or less.

[0127] The method of supplying the plant growth promoter of the present invention is not particularly limited if it is applied to the plant in a manner that enables the effects of the present invention to be achieved.

[0128] That is, there are no particular limitations as long as the above composition comes into contact with or is delivered to the soil of the plant body or the plant rhizosphere of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals. Examples include spraying, irrigating, ploughing, spraying on plant leaves, mixing with fertilizers, adding to hydroponic solutions, or coating or applying to seeds before sowing (e.g., seed coating), but it is preferred to use it as a spray solution obtained by diluting the ingredients of the present invention with water, and foliar spraying is particularly preferred.

[0129] In addition, the spray solution can be prepared at the time of application, and the dilution water used at this time can be any kind of agricultural water, well water, groundwater, river water, lake water, tap water, etc.

[0130] There are no particular limitations on the method of spraying; for example, atomization, i.e., spraying the liquid in a mist form, can be used. Using this method, the plant growth promoter of the present invention adheres to the plant and exhibits good spreadability on the plant.

[0131] As a method of spraying using a spraying method, specific examples include spraying manually using sprayers, sprayers, spraying machines (e.g., nozzle sprayers), or spraying from the air using airplanes, helicopters, drones, etc.

[0132] The dosage of the plant growth promoter of the present invention depends on the concentration of components (A) to (C) contained in the composition at the time of application. For example, when the concentration of component (A) in the spray solution is 100 to 300,000 ppm by mass, the dosage of component (A) per plant is preferably 1 mg or more, more preferably 5 mg or more, more preferably 10 mg or more, and preferably 150 mg or less, more preferably 100 mg or less, more preferably 50 mg or less. Furthermore, it is preferably 1 to 150 mg, more preferably 5 to 100 mg, more preferably 10 to 50 mg. The plant growth promoter can be applied in a single application within the above-mentioned range or in multiple applications.

[0133] The timing and frequency of application vary depending on the plant species, etc. Generally, when applying by spraying, irrigating, ploughing or seed coating onto the surface of cultivation substrates such as soil, it is preferred to apply once or 1 to 3 times before or at the same time as sowing. When applying after sowing, it is preferred to apply between the reproductive growth period and the early vegetative growth period.

[0134] Regarding the above-described embodiments, the present invention also discloses the following methods.

[0135] <1> A plant growth promoter selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the promoter is composed of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, wherein when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or its salt is 10 to 3,000,000.

[0136] <2> A plant growth promoter selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the promoter is composed of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (C) the organic solvent is 4 to 100,000.

[0137] <3> A plant yield enhancer selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the enhancer is composed of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, wherein when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or its salt is 10 to 3,000,000.

[0138] <4> A plant yield enhancer selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the enhancer is composed of (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (C) the organic solvent is 4 to 100,000.

[0139] <5> The agent as described in any one of <1> to <4>, wherein the ratio (mass ratio) of (A) ascorbic acid or its salt and (C) organic solvent is 10 to 3,000,000, preferably 100 to 2,000,000, more preferably 500 to 200,000, and component (C) is 4 to 100,000, preferably 10 to 50,000, more preferably 20 to 20,000, and even more preferably 50 to 10,000 when component (B) is set to 1.

[0140] <6> The agent as described in any one of <1> to <5>, wherein (B) the antioxidant further comprises one or more selected from sodium sulfite, potassium sulfite, glutathione and uric acid.

[0141] <7> The agent as described in any one of <1> to <6>, wherein (C) the organic solvent is one or more selected from isobutanol, dimethyl sulfoxide and glycerol.

[0142] <8> An agent as described in any one of <1> to <7>, wherein it is further composed of (D) a surfactant.

[0143] <9> The formulation as described in <8>, wherein when component (B) is set to 1, the proportion (mass ratio) of surfactant (D) is preferably 10 to 300,000, more preferably 50 to 100,000, and even more preferably 100 to 5,000.

[0144] <10> The agent as described in <8> or <9>, wherein (D) the surfactant comprises at least a nonionic surfactant.

[0145] <11> The agent as described in <10>, wherein (D) the nonionic surfactant is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters and polyoxyethylene alkyl ethers.

[0146] <12> The agent as described in <10>, wherein (D) the surfactant further comprises anionic surfactant or amphoteric surfactant.

[0147] <13> The agent as described in <12>, wherein (D) the nonionic surfactant is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters and polyoxyethylene alkyl ethers, the anionic surfactant is selected from one or more of fatty acid salts, aromatic sulfonate formaldehyde condensates and alkyl sulfates, and the amphoteric surfactant is 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazoline betaine.

[0148] <14> An agent as described in any one of <1> to <13>, wherein it is further combined with (E) a chelating agent.

[0149] <15> The agent as described in <14>, wherein the proportion (mass ratio) of (A) ascorbic acid or its salt, (C) organic solvent, (D) surfactant and (E) chelating agent, when component (B) is set to 1, is as follows: component (A) is 10 to 3,000,000, preferably 100 to 2,000,000, more preferably 500 to 200,000; component (C) is 4 to 100,000, preferably 10 to 50,000, more preferably 20 to 20,000, further preferably 50 to 10,000; component (D) is preferably 10 to 300,000, more preferably 50 to 100,000, further preferably 100 to 5,000; and component (E) is preferably 0.01 to 100, more preferably 0.1 to 100, more preferably 1 to 100, further preferably 1 to 10.

[0150] <16> The agent as described in <14> or <15>, wherein (E) the chelating agent is one or more selected from ethylenediaminetetraacetic acid or its salt and ethylenediamine-N,N'-disuccinic acid.

[0151] <17> The agent as described in any one of <1> to <16>, wherein it is further combined with (F) a carrier.

[0152] <18> The agent as described in <17>, wherein when component (B) is set to 1, the proportion (mass ratio) of carrier (F) is preferably 50 or more, more preferably 100 or more, further preferably 250 or more, and preferably 1,000 or less, more preferably 750 or less, further preferably 650 or less, or preferably 50 to 1,000, more preferably 100 to 750, further preferably 250 to 650.

[0153] <19> The agent as described in <17> or <18>, wherein (F) the carrier is one or more selected from zeolite, silica, bentonite and mirabilite.

[0154] <20> An agent as described in any one of <1> to <17>, wherein it comprises a first agent containing said component (A) and a second agent containing said components (B) and (C), and the two are combined when used.

[0155] <21> A method for promoting the growth of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the method includes the step of applying (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent in combination with water to soil or plants, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or its salt is 10 to 3,000,000.

[0156] <22> A method for increasing the yield of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables and cereals, wherein the method includes the step of applying (A) ascorbic acid or its salt, (B) an antioxidant and (C) an organic solvent in combination with water to soil or plants, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or its salt is 10 to 3,000,000.

[0157] <23> The method as described in <21> or <22>, wherein a first agent containing said ingredient (A) and a second agent containing said ingredients (B) and (C) are prepared, and the two are combined and applied to soil or plants when used.

[0158] <24> The agent as described in <20>, wherein when the second agent is set to 1, the weight ratio of the first agent is 1 to 100.

[0159] <25> The method described in <23>, wherein the first agent and the second agent are dissolved in water for use.

[0160] <26> The method as described in <25>, wherein the concentration of the first agent in the aqueous solution is more than 100 ppm by mass and less than 300,000 ppm by mass, and the concentration of the second agent is more than 10 ppm by mass and less than 100,000 ppm by mass.

[0161] <27> Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a plant growth promoter selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols having 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0162] <28> Use of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a plant growth promoter selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (C) the organic solvent is 4 to 100,000.

[0163] <29> Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a yield enhancer for plants selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols having 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (A) ascorbic acid or a salt thereof is 10 to 3,000,000.

[0164] <30> Use of (A) ascorbic acid or a salt thereof, (B) an antioxidant and (C) an organic solvent in the manufacture of a yield enhancer for plants selected from fruits, vegetables, leafy vegetables, root vegetables and cereals, wherein (B) the antioxidant is at least one selected from tocopherol, butylated hydroxytoluene and butylated hydroxyanisole, and (C) the organic solvent is at least one selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide and polyols, and when component (B) is set to 1, the proportion (mass ratio) of (C) the organic solvent is 4 to 100,000.

[0165] <31> The use as described in any one of <27> to <30>, wherein the ratio (mass ratio) of (A) ascorbic acid or its salt and (C) organic solvent is 10 to 3,000,000, preferably 100 to 2,000,000, more preferably 500 to 200,000, and component (C) is 4 to 100,000, preferably 10 to 50,000, more preferably 20 to 20,000, and even more preferably 50 to 10,000 when component (B) is set to 1.

[0166] <32> The use as described in any one of <27> to <30>, wherein (B) the antioxidant further comprises one or more selected from sodium sulfite, potassium sulfite, glutathione and uric acid.

[0167] <33> The use as described in any one of <27> to <30>, wherein (C) the organic solvent is one or more selected from isobutanol, dimethyl sulfoxide and glycerol.

[0168] <34> The use as described in any one of <27> to <30>, wherein it is further combined with (D) surfactant.

[0169] <35> As described in <34>, wherein when component (B) is set to 1, the proportion (mass ratio) of surfactant (D) is preferably 10 to 300,000, more preferably 50 to 100,000, and even more preferably 100 to 5,000.

[0170] <36> As described in <34> or <35>, wherein (D) the surfactant comprises at least a nonionic surfactant.

[0171] <37> As described in <36>, wherein (D) the nonionic surfactant is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters and polyoxyethylene alkyl ethers.

[0172] <38> As described in <36>, wherein (D) the surfactant further comprises anionic surfactants or amphoteric surfactants.

[0173] <39> As described in <38>, wherein (D) the nonionic surfactant is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters and polyoxyethylene alkyl ethers, the anionic surfactant is selected from one or more of fatty acid salts, aromatic sulfonate formaldehyde condensates and alkyl sulfates, and the amphoteric surfactant is 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazoline betaine.

[0174] <40> The use as described in any one of <27> to <39>, wherein it is further combined with (E) a chelating agent.

[0175] <41> As described in <40>, wherein the proportion (by mass) of (A) ascorbic acid or its salt, (C) organic solvent, (D) surfactant and (E) chelating agent, when component (B) is set to 1, is as follows: component (A) is 10 to 3,000,000, preferably 100 to 2,000,000, more preferably 500 to 200,000; component (C) is 4 to 100,000, preferably 10 to 50,000, more preferably 20 to 20,000, further preferably 50 to 10,000; component (D) is preferably 10 to 300,000, more preferably 50 to 100,000, further preferably 100 to 5,000; and component (E) is preferably 0.01 to 100, more preferably 0.1 to 100, more preferably 1 to 100, further preferably 1 to 10.

[0176] <42> As described in <40> or <41>, wherein (E) the chelating agent is one or more selected from ethylenediaminetetraacetic acid or its salts and ethylenediamine-N,N'-disuccinic acid.

[0177] <43> The use as described in any one of <27> to <42>, wherein it is further combined with (F) carrier.

[0178] <44> As described in <43>, when component (B) is set to 1, the proportion (mass ratio) of carrier (F) is preferably 50 to 1,000, more preferably 100 to 750, and even more preferably 250 to 650.

[0179] <45> As described in <43> or <44>, wherein (F) the carrier is one or more selected from zeolite, silica, bentonite and mirabilite.

[0180] <46> The use as described in any one of <27> to <30>, wherein the first agent comprising said ingredient (A) and the second agent comprising said ingredients (B) and (C) are combined in use.

[0181] <47> Among <1>, <2>, <3>, <4>, <21>, <22>, <27>, <28>, <29> and <30>, fruits and vegetables are preferably from the Cucurbitaceae, Solanaceae, Rosaceae, Malvaceae or Poaceae families; leaf and stem vegetables are preferably from the Brassicaceae, Asteraceae, Amaranthaceae, Apiaceae, Amaryllidaceae, Rosaceae or Perillaaceae families; and root vegetables are preferably from the Solanaceae, Apiaceae, Brassicaceae, Asteraceae, Amaranthaceae or Convolvulaceae families.

[0182] <48> Among <1>, <2>, <3>, <4>, <21>, <22>, <27>, <28>, <29> and <30>, the preferred fruits and vegetables are cucumber, bitter melon, cantaloupe, pumpkin, tomato, strawberry, wild strawberry or okra; the preferred leafy and stem vegetables are cabbage, komatsuna, lettuce, garland chrysanthemum, spinach, celery, scallion, leek, salsa burnet or perilla; the preferred root vegetables are potato, carrot, radish, burdock or beet; and the preferred grains are rice, wheat or corn.

[0183] Example

[0184] Example 1: Effects of the ascorbic acid composition on the growth of cabbage

[0185] (1) Soil preparation

[0186] Mid-term fertility potting mix (TAKII aqueous cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.) were mixed at a volume ratio of 1:1, and the mixture was filled into plastic seedling pots (10.5 cm in diameter and 9 cm in height). Each pot was supplied with 200 mL of tap water before transplanting cabbage seedlings purchased from a home improvement store. Furthermore, the number of replicates for each experimental area was set to 4 (n = 4).

[0187] (2) Cultivation conditions

[0188] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0189] (3) Foliar spraying treatment

[0190] Prepare the spray solution shown below and spray 6.7 mL onto each plant using a spray bottle on the 10th day after transplanting. Foliar spraying was performed only once in three experimental areas.

[0191] Regarding the preparation of the spray solution, agent 1 is prepared by pre-dissolving BHT in component (B) in component (C), then mixing component (C) with component (D). Component (A) is used as agent 2. Agent 1 is dissolved in water, and then agent 2 is dissolved to prepare the spray solution.

[0192] Here, ascorbic acid was "Food Additive Grade Vitamin C (L-Ascorbic Acid) Fine Mesh Type SSS" manufactured by Fuso Chemical Industry Co., Ltd. BHT and isobutanol were manufactured by Fujifilm and Kojun Pharmaceutical Co., Ltd. Sorbitan monolaurate was used as a nonionic surfactant. Test areas 1-3 for evaluation are shown below.

[0193] On day 18 after transplanting, the fresh weight of the aboveground parts of the plant was measured.

[0194] <Spray liquid>

[0195] • Zone 1: Control (no application)

[0196] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0197] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0198] (4) Results

[0199] The results of the determination of the fresh weight of the aboveground parts are expressed as follows: Figure 1 The bar chart in the figure represents the mean ± standard deviation. In cabbage, a comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a greater growth-promoting effect compared to using ascorbic acid alone. In test area 3, the average fresh weight of the aboveground parts increased by 9% compared to test area 1.

[0200] Example 2: Effects of the ascorbic acid composition on the growth of Komatsuna (Japanese mustard greens).

[0201] (1) Soil preparation

[0202] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-based cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (Akagi Engei Co., Ltd.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and Komatsuna seeds (variety: Misugi, SakataSeed Corporation) were sown. Four seeds were sown in each tray, one seed approximately 5 mm below the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0203] (2) Cultivation conditions

[0204] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0205] (3) Foliar spraying treatment

[0206] Prepare the spray solution shown below and apply it to the leaves using a sprayer on the 21st day after sowing. Foliar spraying was performed only once in six experimental zones. 6.7 mL was sprayed per plant in zones 1–4, and 0.125 mL was sprayed per plant in zones 5 and 6.

[0207] Regarding the preparation of the spray solution, BHT in component (B) was pre-dissolved in component (C), and then component (C) and component (D) were mixed to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used, along with isopropanol (manufactured by Fujifilm and Koichi Chemical Co., Ltd.), dimethyl sulfoxide (manufactured by Fujifilm and Koichi Chemical Co., Ltd.), polyoxyethylene resin ester, potassium salt of fatty acid soap, polyoxyethylene sorbitan monooleate, polyethylene glycol monolaurate, and glycerol fatty acid ester. The evaluation test areas 1 to 3 are described below.

[0208] On the 28th day after sowing, the aboveground parts of the plant were dried at 90°C for 24 hours, and the dried weight of the aboveground parts was measured.

[0209] <Spray liquid>

[0210] Zone 1: Control (tap water only)

[0211] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0212] Zone 3: Ascorbic acid 1,500 ppm + BHT 1 ppm + Isobutanol 100 ppm + Sorbitan monolaurate 350 ppm + Polyoxyethylene resin ester 28 ppm + Fatty acid soap potassium salt 22 ppm. Foliar spray (dissolve in tap water).

[0213] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isopropanol 115 ppm + polyoxyethylene sorbitan monooleate 258 ppm (dissolve in tap water).

[0214] Zone 5: Foliar spray with ascorbic acid 150,000 ppm (dissolved in tap water).

[0215] • Zone 6: Foliar spray with 150,000 ppm ascorbic acid + 1 ppm BHT + 8,000 ppm dimethyl sulfoxide + 24,000 ppm polyethylene glycol monolaurate + 16,000 ppm glycerol fatty acid esters (dissolve in tap water).

[0216] (4) Results

[0217] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 2 The bar chart in the figure represents the mean ± standard deviation. In Komatsuna, the comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to test area 1, the average increase in aboveground dry weight was 3% in test area 3, 12% in test area 4, and 3% in test area 6. These results indicate that the use of component (C) in spraying, in addition to isobutanol, with the addition of isopropanol and dimethyl sulfoxide, also results in a growth-promoting effect. Furthermore, it is shown that component (D), in addition to sorbitan monolaurate, also yields a growth-promoting effect when using polyoxyethylene resin esters, potassium salts of fatty acids, polyoxyethylene sorbitan monooleate, polyethylene glycol monolaurate, and glycerol fatty acid esters. Moreover, the results from test area 6 confirm that a growth-promoting effect can also be obtained with high concentrations and low water volumes during spraying.

[0218] Example 3: Effects of the ascorbic acid composition on the growth of spinach

[0219] (1) Soil preparation

[0220] Mid-term fertility-enhancing potting mix (TAKII hydroponic mid-term fertility-enhancing potting mix, TAKII Seedling Co., Ltd.) was mixed with fine vermiculite (Akagi Engei Co., Ltd.) at a volume ratio of 1:1. This mixture was then filled into plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was given 200 mL of tap water and then transplanted with spinach seedlings (variety: Super Arena 7, Tohoku Seed Co., Ltd.) purchased from a home improvement store. Furthermore, the number of replicates for each experimental area was set to 4 (n = 4).

[0221] (2) Cultivation conditions

[0222] Cultivation was carried out indoors, with cultivation conditions set as follows: 12 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0223] (3) Foliar spraying treatment

[0224] Prepare the spray solution shown below and spray 6.7 mL per plant using a spray bottle on day 18 after transplanting. Foliar spraying was performed only once in three experimental areas.

[0225] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0226] The fresh weight of the aboveground parts of the plant was measured on day 25 after transplanting.

[0227] <Spray liquid>

[0228] • Zone 1: Control (no application)

[0229] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0230] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0231] (4) Results

[0232] The results of the determination of the fresh weight of the aboveground parts are expressed as follows: Figure 3 The bars in the figure represent the mean ± standard deviation. In spinach, the comparison between test areas 2 and 3 shows that by adding components (B), (C), and (D) to ascorbic acid, a greater growth-promoting effect can be obtained than when ascorbic acid is used alone. Compared with test area 1, the average fresh weight of the aboveground parts increased by 5% in test area 3.

[0233] Example 4: Effects of the ascorbic acid composition on strawberry growth

[0234] (1) Soil preparation

[0235] After filling 2L of bottom stones (the stone mesh packaged at the bottom of the pot, Nature Applied Science Co., Ltd.) into flowerpots (width 65.3cm, depth 24.5cm, height 18.5cm), 9L of mid-term fertilization potting mix (TAKII water-containing cellular potting mix, mid-term fertilization type, TAKII Seedling Co., Ltd.) was added. Each flowerpot was then supplied with 2L of tap water before transplanting strawberry seedlings (variety: Dolce Berry, Suntory Flowers Co., Ltd.) purchased from a home improvement store. Furthermore, the number of replicates for each experimental area was set to 3 (n=3).

[0236] (2) Cultivation conditions

[0237] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigate with 1L of tap water about 3 times a week.

[0238] (3) Foliar spraying treatment

[0239] Prepare the spray solution shown below and spray 6.7 mL per plant using a spray bottle on day 17 after transplanting. Foliar spraying was performed only once in three experimental areas.

[0240] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The test areas 1 to 3 for evaluation are described below.

[0241] The aboveground dry weight of the plant was measured on day 49 after transplanting.

[0242] <Spray liquid>

[0243] • Zone 1: Control (no application)

[0244] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0245] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0246] (4) Results

[0247] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 4The bar chart in the figure represents the mean ± standard deviation. The comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to test area 1, the average fresh weight of the aboveground parts increased by 8% in test area 3.

[0248] Example 5: Effects of the ascorbic acid composition on the growth of lettuce

[0249] (1) Soil preparation and cultivation conditions

[0250] Fill plastic seedling trays (7.5cm in diameter, 6.5cm in height) with initial-stage fertile potting mix (TAKII Cellular Culture TM-1, TAKII Seedling Co., Ltd.). Use "Great Lake" (Atariya Farm Co., Ltd.) lettuce seeds. After adding 100mL of tap water to each tray, sow 4 seeds, just enough to cover them, in each tray. After germination, thin the seedlings to one plant per tray. On the 28th day after sowing, transplant the plants into plastic seedling trays (10.5cm in diameter, 9cm in height) filled with a 1:1 mixture of mid-stage fertile potting mix (TAKII Water-Containing Cellular Culture Mid-Stage Fertilizer, TAKII Seedling Co., Ltd.) and fine vermiculite (AkagiEngei Co., Ltd.), adding 250mL of tap water to each tray.

[0251] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 Irrigate with 600mL of tap water approximately twice a week.

[0252] In addition, the number of replicates for each experimental area was set to 6 (n=6).

[0253] (2) Foliar spraying treatment

[0254] Prepare the following spray solution and spray 6.7 mL per plant using a spray bottle on the second day after transplanting (30 days after sowing). Foliar spraying was performed only once in three experimental areas.

[0255] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0256] On the 9th day after transplanting (37 days after sowing), the fresh weight of the aboveground parts of the plant was measured.

[0257] <Spray liquid>

[0258] • Zone 1: Control (no application)

[0259] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in deionized water)

[0260] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0261] (3) Results

[0262] The results of the determination of the fresh weight of the aboveground parts are expressed as follows: Figure 5 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental zones 2 and 3 shows that in lettuce, the addition of components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. In experimental zone 4, the average fresh weight of the aboveground parts increased by 2.7% compared to experimental zone 1.

[0263] Example 6: Effect of the ascorbic acid composition on the growth of onions

[0264] (1) Soil preparation and cultivation conditions

[0265] Fill plastic seedling trays (7.5cm in diameter, 6.5cm in height) with initial-stage fertile potting mix (TAKII Cellular Culture TM-1, TAKII Seedling Co., Ltd.). Use "Kujo Onion" (Atariya Agricultural Co., Ltd.) seeds. After adding 100mL of tap water to each tray, sow 4 seeds, 1 seed at a depth of about 5mm from the soil surface. After germination, thin the seedlings to 1 plant per tray. On the 34th day after sowing, transplant the plants into plastic seedling trays (10.5cm in diameter, 9cm in height) filled with soil containing a 1:1 volume ratio of mid-stage fertile potting mix (TAKII Water-Containing Cellular Culture Mid-Stage Fertilizer, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.), adding 250mL of tap water to each tray.

[0266] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 Irrigate with 600mL of tap water approximately twice a week.

[0267] In addition, the number of replicates for each experimental area was set to 6 (n=6).

[0268] (2) Foliar spraying treatment

[0269] Prepare the spray solution shown below and spray 6.7 mL per plant using a spray bottle on the second day after transplanting (36 days after sowing). Foliar spraying was performed only once in three experimental areas.

[0270] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0271] The fresh weight of the aboveground parts of the plant was measured on the 9th day after transplanting (43 days after sowing).

[0272] <Spray liquid>

[0273] • Zone 1: Control (no application)

[0274] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0275] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0276] (3) Results

[0277] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 6 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental areas 2 and 3 shows that in scallions, adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental area 1, the average fresh weight of the aboveground parts increased by 9.3% in experimental area 3.

[0278] Example 7: Effect of the ascorbic acid composition on the growth of onions

[0279] (1) Soil preparation

[0280] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (Akagi Engei Co., Ltd.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and onion seeds (variety: Ishikura onion, Kaneko Seeds Co., Ltd.) were sown. Four seeds were sown one at a depth of approximately 5 mm from the soil surface in each tray. After germination, seedlings were thinned to three plants per tray. The replicates for each experimental area were set to 12 (n = 12).

[0281] (2) Cultivation conditions

[0282] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0283] (3) Foliar spraying treatment

[0284] The following spray solution was prepared for foliar spraying. Five test areas were studied. In test areas 1-4, foliar spraying was performed only once on day 21 after sowing, with 6.7 mL sprayed per pot (3 plants). In test area 5, foliar spraying was performed twice, once on day 21 and once on day 26 after sowing, with 6.7 mL sprayed per pot. The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The reagents were the same as in Example 2.

[0285] On the 28th day after sowing, the fresh weight of the above-ground parts of the plant was measured.

[0286] <Spray liquid>

[0287] Zone 1: Control (tap water only)

[0288] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0289] Zone 3: Ascorbic acid 1,500 ppm + BHT 1 ppm + Isobutanol 100 ppm + Sorbitan monolaurate 350 ppm + Polyoxyethylene resin ester 28 ppm + Fatty acid soap potassium salt 22 ppm. Foliar spray (dissolve in tap water).

[0290] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isopropanol 115 ppm + polyoxyethylene sorbitan monooleate 258 ppm (dissolve in tap water).

[0291] Zone 5: Ascorbic acid 1,500 ppm + BHT 1 ppm + Isobutanol 100 ppm + Sorbitan monolaurate 350 ppm + Polyoxyethylene resin ester 28 ppm + Fatty acid soap potassium salt 22 ppm. Foliar spray (dissolve in tap water).

[0292] (4) Results

[0293] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 7 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental areas 2 and 3 shows that in scallions, adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental area 1, the average fresh weight of the aboveground parts increased by 3.5% in experimental area 3.

[0294] Example 8: Effects of the ascorbic acid composition on the growth of carrots

[0295] (1) Soil preparation and cultivation conditions

[0296] Fill plastic seedling trays (7.5cm in diameter, 6.5cm in height) with initial-stage fertile potting mix (TAKII Cellular Culture TM-1, TAKII Seedling Co., Ltd.). Use "Xiangyang No. 2" carrot seeds (TAKII Seedling Co., Ltd.). After adding 100mL of tap water to each tray, sow 4 seeds one at a depth of about 5mm from the soil surface. After germination, thin the seedlings to one plant per tray. On the 34th day after sowing, transplant the plants into plastic seedling trays (10.5cm in diameter, 9cm in height) filled with soil containing a 1:1 volume ratio of mid-stage fertile potting mix (TAKII Water-Containing Cellular Culture Mid-Stage Fertilizer, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.), adding 250mL of tap water to each tray.

[0297] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 Irrigate with 600mL of tap water approximately twice a week.

[0298] In addition, the number of replicates for each experimental area was set to 6 (n=6).

[0299] (2) Foliar spraying treatment

[0300] Prepare the spray solution and spray 6.7 mL per plant using a spray bottle on the second day after transplanting (36 days after sowing). Foliar spraying was performed only once in three experimental areas.

[0301] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0302] The fresh weight of the underground part of the plant was measured on the 9th day after transplanting (43 days after sowing).

[0303] <Spray liquid>

[0304] • Zone 1: Control (no application)

[0305] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0306] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0307] (3) Results

[0308] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 8 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental zones 2 and 3 shows that in carrots, the addition of components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to experimental zone 1, the average fresh weight of the underground parts increased by 6.7% in experimental zone 3.

[0309] Example 9: Effect of the ascorbic acid composition on potato yield

[0310] (1) Soil preparation and cultivation conditions

[0311] Fill flowerpots (65.3cm wide, 24.5cm deep, 18.5cm high) with 6L of mid-term fertility potting mix (TAKII water-based cellular potting mix, TAKII Seedling Co., Ltd.). After adding 500mL of tap water to each pot, plant seed potatoes (variety: Baron). The number of replicates for each experimental area was set to 3 (n=3). Cultivation was carried out outdoors.

[0312] (2) Foliar spraying treatment

[0313] Prepare the spray solution shown below and spray 6.7 mL per plant using a spray bottle on the 72nd day after seed potato planting. Foliar spraying was performed only once in three experimental areas.

[0314] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0315] The fresh weight of each seed potato was measured 84 days after planting.

[0316] <Spray liquid>

[0317] • Zone 1: Control (no application)

[0318] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0319] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0320] (3) Results

[0321] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 9 The bar chart in the figure represents the mean ± standard deviation. The comparison between test areas 2 and 3 shows that in potatoes, the addition of components (B), (C), and (D) to ascorbic acid results in higher yields than when ascorbic acid is used alone. In test area 3, the average fresh weight of the aboveground parts increased by 7% compared to test area 1.

[0322] Example 10: Effect of the ascorbic acid composition on tomato yield

[0323] (1) Soil preparation

[0324] After filling 2L of bottom stones into flowerpots (65.3cm wide, 24.5cm deep, 18.5cm high), 9L of mid-term fertilization potting mix (TAKII water-based cellular potting mix, TAKII Seedling Co., Ltd.) was added. Each flowerpot was then supplied with 2L of tap water before transplanting tomato seedlings (variety: Home Momotaro, TAKII Seedling Co., Ltd.) purchased from a home improvement store. Additionally, the number of replicates for each experimental area was set to 3 (n=3).

[0325] (2) Foliar spraying treatment

[0326] Prepare the following spray solution and spray 6.7 mL per tomato seedling using a spray bottle on the 22nd day after planting. Foliar spraying was performed only once in three experimental areas.

[0327] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0328] Tomatoes were harvested from 35 to 78 days after planting, and the fresh weight of each tomato plant was measured.

[0329] <Spray liquid>

[0330] • Zone 1: Control (no application)

[0331] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0332] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0333] (3) Results

[0334] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 10 The bar chart in the figure represents the mean ± standard deviation. The comparison between test areas 2 and 3 shows that in tomatoes, by adding components (B), (C), and (D) to ascorbic acid, larger fruits can be obtained per fruit than when ascorbic acid is used alone. Compared to test area 1, in test area 3, the average fresh weight of each tomato fruit increased by 40%.

[0335] Example 11 Effect of ascorbic acid composition on tomato yield

[0336] (1) Soil preparation and cultivation conditions

[0337] Fill plastic seedling trays (7.5cm in diameter, 6.5cm in height) with initial-stage fertile potting mix (TAKII Cellular Culture TM-1, TAKII Seedling Co., Ltd.). Use "Micro-Tom" tomato seeds. After adding 100mL of tap water to each tray, sow two seeds at a depth of about 5mm from the soil surface. After germination, thin the seedlings to one plant per tray. On the 32nd day after sowing, transplant the plants into plastic seedling trays (13.5cm in diameter, 11cm in height) filled with a 1:1 volume mixture of mid-stage fertile potting mix (TAKII Water-Containing Cellular Culture Mid-Stage Fertilizer, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.). After adding 375mL of tap water to each tray, transplant the plants.

[0338] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation with 600mL of tap water approximately twice a week. Additionally, the number of replicates for each experimental area was set to 4 (n=4).

[0339] (2) Foliar spraying treatment

[0340] Prepare the following spray solution and spray 6.7 mL per plant using a spray bottle on the second day after transplanting (34 days after sowing). Foliar spraying was performed only once in three experimental areas.

[0341] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0342] Harvest was carried out between day 46 (day 80 after sowing) and day 73 (day 107 after sowing), and the fresh weight of the fruit was measured.

[0343] <Spray liquid>

[0344] • Zone 1: Control (no application)

[0345] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0346] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0347] (3) Results

[0348] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 11 The bar chart in the figure represents the mean ± standard deviation. The comparison between test areas 2 and 3 shows that in tomatoes, by adding components (B), (C), and (D) to ascorbic acid, larger fruits per fruit can be obtained than when ascorbic acid is used alone. Compared to test area 1, in test area 3, the average fresh weight of each tomato fruit increased by 3.4%.

[0349] Example 12 Effect of ascorbic acid composition on cucumber yield

[0350] (1) Soil preparation

[0351] After filling 2L of bottom stones into flowerpots (65.3cm wide, 24.5cm deep, 18.5cm high), 9L of mid-term fertilization potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) was added. Each flowerpot was then supplied with 2L of tap water before transplanting cucumber seedlings (variety: disease-resistant summer-growing cucumber, Nikko Seedling Co., Ltd.) purchased from a home improvement store. Furthermore, the number of replicates for each experimental area was set to 3 (n=3).

[0352] (2) Foliar spraying treatment

[0353] Prepare the following spray solution and spray 6.7 mL per seedling using a spray bottle on the 22nd day after cucumber seedling planting. Foliar spraying was performed only once in three experimental areas.

[0354] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The types of reagents were the same as in Example 1. The evaluation test areas 1 to 3 are described below.

[0355] The cucumbers were harvested from day 28 to day 63 after planting, and the number of fruits per plant was measured.

[0356] <Spray liquid>

[0357] • Zone 1: Control (no application)

[0358] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0359] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0360] (3) Results

[0361] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 12 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental areas 2 and 3 shows that in cucumbers, by adding components (B), (C), and (D) to ascorbic acid, more fruits per plant can be obtained than when ascorbic acid is used alone. In experimental area 3, the number of fruits per cucumber plant increased by 1.5 times compared to experimental area 1.

[0362] Example 13 Effects of the ascorbic acid composition on rice growth

[0363] (1) Soil preparation

[0364] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (Akagi Engei Co., Ltd.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and rice seeds (variety: Koshihikari) were sown. Five seeds were sown one at a depth of approximately 5 mm from the soil surface in each tray. After germination, seedlings were thinned to two plants per tray. The replicates for each experimental area were set to 12 (n = 12).

[0365] (2) Cultivation conditions

[0366] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0367] (3) Foliar spraying treatment

[0368] Prepare the following spray solution for foliar spraying. Three test areas were studied. Foliar spraying was performed only once on day 21 after sowing, with 6.7 mL sprayed per pot. The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The reagents used were the same as in Example 2.

[0369] The aboveground dry weight of the plant was measured on the 28th day after sowing.

[0370] <Spray liquid>

[0371] Zone 1: Control (tap water only)

[0372] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0373] Zone 3: Ascorbic acid 1,500 ppm + BHT 1 ppm + Isobutanol 100 ppm + Sorbitan monolaurate 350 ppm + Polyoxyethylene resin ester 28 ppm + Fatty acid soap potassium salt 22 ppm. Foliar spray (dissolve in tap water).

[0374] (4) Results

[0375] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 13 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental areas 2 and 3 shows that in rice, adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental area 1, the average dry weight of the aboveground parts increased by 2.7% in experimental area 3.

[0376] Example 14 Effects of the ascorbic acid composition on wheat growth

[0377] (1) Soil preparation

[0378] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and wheat seeds (variety: Nōrin 61) were sown. Five seeds were sown one at a depth of approximately 5 mm from the soil surface in each tray. After germination, seedlings were thinned to two plants per tray. The replicates for each experimental area were set to 12 (n = 12).

[0379] (2) Cultivation conditions

[0380] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0381] (3) Foliar spraying treatment

[0382] Prepare the following spray solution for foliar spraying. Three test areas were studied. Foliar spraying was performed only once on day 21 after sowing, with 6.7 mL sprayed per pot. The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The reagents used were the same as in Example 2.

[0383] The aboveground dry weight of the plant was measured on the 28th day after sowing.

[0384] <Spray liquid>

[0385] Zone 1: Control (tap water only)

[0386] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0387] Zone 3: Ascorbic acid 1,500 ppm + BHT 1 ppm + Isobutanol 100 ppm + Sorbitan monolaurate 350 ppm + Polyoxyethylene resin ester 28 ppm + Fatty acid soap potassium salt 22 ppm. Foliar spray (dissolve in tap water).

[0388] (4) Results

[0389] The results of the determination of the dry weight of the aboveground parts are expressed as follows: Figure 14 The bar chart in the figure represents the mean ± standard deviation. The comparison between experimental areas 2 and 3 shows that in rice, adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental area 1, the average dry weight of the aboveground parts increased by 0.6% in experimental area 3.

[0390] Example 15: Effects of the ascorbic acid composition on the growth of leeks

[0391] (1) Soil preparation

[0392] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.) was mixed and filled into plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and chive seeds (variety: large-leaf chives, Atariya Agricultural Co., Ltd.) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The replicates for each experimental area were set to 6 (n = 6).

[0393] (2) Cultivation conditions

[0394] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0395] (3) Foliar spraying treatment

[0396] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on day 52 after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0397] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0398] <Spray liquid>

[0399] Zone 1: Control (tap water only)

[0400] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0401] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0402] (4) Results

[0403] On day 57 after sowing, the aboveground parts of the plants were dried at 90°C for 48 hours, and the dried weight of the aboveground parts was measured. The results of the aboveground part drying weight measurement are expressed as follows: Figure 15 The bar chart in the figure represents the mean ± standard deviation. In chives, the comparison between experimental zones 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to experimental zone 1, the average dry weight of the aboveground parts increased by approximately 15.1% in experimental zone 2 and by approximately 35.6% in experimental zone 3.

[0404] Example 16 Effect of ascorbic acid composition on the growth of radishes

[0405] (1) Soil preparation

[0406] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (Akagi Engei Co., Ltd.) was used to fill plastic seedling trays (10.5 cm in diameter, 12 cm in height). Each tray was supplied with 250 mL of tap water, and small radish seeds (variety: Isabel, KanekoSeeds Co., Ltd.) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 1 cm from the soil surface. After germination, thinning was performed so that each tray contained one plant. The number of replicates for each experimental area was set to 6 (n = 6).

[0407] (2) Cultivation conditions

[0408] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0409] (3) Foliar spraying treatment

[0410] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 20th day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0411] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0412] <Spray liquid>

[0413] Zone 1: Control (tap water only)

[0414] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0415] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0416] (4) Results

[0417] On day 27 after sowing, the fresh weight of the underground parts of the plant was measured. The results of the fresh weight measurement of the underground parts are expressed as follows: Figure 16 The bar chart in the figure represents the mean ± standard deviation. In radishes, the comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to test area 1, the average increase in aboveground dry weight was approximately 2.3% in test area 2 and approximately 15.6% in test area 3.

[0418] Example 17 Effect of ascorbic acid composition on the growth of Sanguisorba officinalis

[0419] (1) Soil preparation

[0420] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (Akagi Engei Co., Ltd.) was mixed and filled into plastic seedling trays (10.5 cm in diameter and 9 cm in height). Each tray was supplied with 250 mL of tap water, and seeds of *Sanguisorba officinalis* (Nikko Seedling Co., Ltd.) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The replicates for each experimental area were set to 6 (n = 6).

[0421] (2) Cultivation conditions

[0422] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0423] (3) Foliar spraying treatment

[0424] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 21st day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0425] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0426] <Spray liquid>

[0427] Zone 1: Control (tap water only)

[0428] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0429] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0430] (4) Results

[0431] On the 28th day after sowing, the plants were dried at 90°C for 48 hours, and the dry weight of the aboveground parts was measured. The results of the aboveground dry weight measurement are expressed as follows: Figure 17 The bar chart in the figure represents the mean ± standard deviation. In *Sanguisorba officinalis*, the comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to test area 1, the average increase in aboveground dry weight was approximately 7.6% in test area 2 and approximately 58.1% in test area 3.

[0432] Example 18 Effect of ascorbic acid composition on the growth of bitter melon

[0433] (1) Soil preparation

[0434] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (Akagi Engei Co., Ltd.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and bitter gourd seeds (Okinawa bitter gourd, Nikko Seedling Co., Ltd.) were sown. Two seeds were sown in each tray, one at a depth of approximately 1 cm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 3 (n = 3).

[0435] (2) Cultivation conditions

[0436] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0437] (3) Foliar spraying treatment

[0438] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 22nd day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0439] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0440] <Spray liquid>

[0441] Zone 1: Control (tap water only)

[0442] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0443] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0444] (4) Results

[0445] On the 29th day after sowing, the plants were dried at 90°C for 48 hours, and the dried weight of the underground parts was measured. The results of the dried weight of the underground parts are expressed as follows: Figure 18 The bar chart in the figure represents the mean ± standard deviation. In bitter gourd, a comparison between test areas 2 and 3 shows that ascorbic acid alone did not confirm a growth-promoting effect, but by adding components (B), (C), and (D) to ascorbic acid, a growth-promoting effect was obtained. Compared to test area 1, in test area 3, the average dry weight of the underground parts increased by approximately 22.6%.

[0446] Example 19 Effect of ascorbic acid composition on the growth of perilla.

[0447] (1) Soil preparation

[0448] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and perilla seeds (red perilla, Nikko Seedling Co., Ltd.) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, thinning was performed so that each tray contained one plant. The replicate number for each experimental area was set to 6 (n = 6).

[0449] (2) Cultivation conditions

[0450] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0451] (3) Foliar spraying treatment

[0452] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 29th day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0453] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0454] <Spray liquid>

[0455] Zone 1: Control (tap water only)

[0456] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0457] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0458] (4) Results

[0459] On day 36 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground fresh weight measurement are expressed as follows: Figure 19The bar chart in the figure represents the mean ± standard deviation. In perilla, the comparison between experimental zones 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to experimental zone 1, the average fresh weight of the aboveground parts increased by approximately 52.4% in experimental zone 2 and by approximately 77.5% in experimental zone 3.

[0460] Example 20 Effect of ascorbic acid composition on the growth of *Cyperus difformis*

[0461] (1) Soil preparation

[0462] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and then 4 seeds of *Trifolium repens* (white stem trefoil, Kaneko Seeds Co., Ltd.) were sown, one seed at a depth of approximately 5 mm from the soil surface, in each tray. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0463] (2) Cultivation conditions

[0464] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0465] (3) Foliar spraying treatment

[0466] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 29th day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0467] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0468] <Spray liquid>

[0469] Zone 1: Control (tap water only)

[0470] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in deionized water).

[0471] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0472] (4) Results

[0473] On day 36 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground fresh weight measurement are expressed as follows: Figure 20 The bar chart in the figure represents the mean ± standard deviation. In *Cyperus difformis*, the comparison between experimental zones 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to experimental zone 1, the average fresh weight of the aboveground parts increased by approximately 38.2% in experimental zone 2 and by approximately 49.6% in experimental zone 3.

[0474] Example 21 Effect of ascorbic acid composition on the growth of burdock

[0475] (1) Soil preparation

[0476] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 12 cm in height). Each tray was supplied with 250 mL of tap water, and burdock seeds (variety: Cobalt Extremely Early Short Type, Utane Co., Ltd.) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, thinning was performed so that each tray contained one plant. The replicate number for each experimental area was set to 6 (n = 6).

[0477] (2) Cultivation conditions

[0478] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0479] (3) Foliar spraying treatment

[0480] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 27th day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0481] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0482] <Spray liquid>

[0483] Zone 1: Control (tap water only)

[0484] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0485] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0486] (4) Results

[0487] On day 34 after sowing, the fresh weight of the underground parts of the plant was measured. The results of the fresh weight measurement of the underground parts are expressed as follows: Figure 21 The bar chart in the figure represents the mean ± standard deviation. In burdock, the comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to test area 1, the average fresh weight of the underground parts increased by approximately 6.9% in test area 2 and by approximately 38.8% in test area 3.

[0488] Example 22 Effect of ascorbic acid composition on the growth of sugar beets

[0489] (1) Soil preparation

[0490] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 12 cm in height). Each tray was supplied with 250 mL of tap water, and beet seeds (variety: Detroit Dark Red, Tohoku Seed Co., Ltd.) were sown. Four seeds were sown one at a depth of approximately 1 cm from the soil surface in each tray. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0491] (2) Cultivation conditions

[0492] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0493] (3) Foliar spraying treatment

[0494] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 27th day after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0495] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0496] <Spray liquid>

[0497] Zone 1: Control (tap water only)

[0498] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0499] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0500] (4) Results

[0501] On day 34 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the fresh weight measurement of the underground parts are expressed as follows: Figure 22 The bar chart in the figure represents the mean ± standard deviation. In sugar beets, a comparison between test areas 2 and 3 shows that ascorbic acid alone did not demonstrate a growth-promoting effect, but the addition of components (B), (C), and (D) to ascorbic acid resulted in a growth-promoting effect. Compared to test area 1, the average fresh weight of the underground parts increased by approximately 35.3% in test area 3.

[0502] Example 23 Effect of ascorbic acid composition on the number of flowers in wild strawberry

[0503] (1) Soil preparation

[0504] A 1:1 volume ratio of mid-term fertility potting mix (TAKII water-based cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (13.5 cm in diameter, 11 cm in height). Each tray was supplied with 250 mL of tap water, and wild strawberry seeds (Sakata Seed Corporation) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 3 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 4 (n = 4).

[0505] (2) Cultivation conditions

[0506] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0507] (3) Foliar spraying treatment

[0508] Prepare the spray solution shown below and apply it to the leaves using a sprayer on day 63 after sowing. Foliar spraying was performed only once in three experimental areas. 6.7 mL was sprayed per plant.

[0509] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas 1 to 3 are described below.

[0510] <Spray liquid>

[0511] Zone 1: Control (tap water only)

[0512] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0513] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0514] (4) Results

[0515] The total number of flowers on each plant was measured from foliar spraying on day 63 after sowing to day 125 after sowing. The results of the flower count measurement are expressed as follows: Figure 23 The bar chart in the figure represents the mean ± standard deviation. In wild strawberry, the comparison between test areas 2 and 3 shows that by adding components (B), (C), and (D) to ascorbic acid, a greater increase in flower number can be obtained than when ascorbic acid is used alone. Compared with test area 1, the average increase in flower number in test area 3 was approximately 63.4%.

[0516] Example 24 Effect of ascorbic acid composition on wheat yield

[0517] (1) Soil preparation

[0518] Soil was filled into 1 / 5000 Warburg pots. 1.2 g of AG MAX (N:P:K = 10:10:10, i-Agri Corp.) was applied per pot at an N:P:K ratio of 6:6:6 (kg / 10a). Eight wheat seeds (Agroin 61, Tsurushin Seed Ltd.) were then sown in two rows per pot. After germination, seedlings were thinned to four plants per pot. The replicates for each experimental area were set to 10 (n = 10).

[0519] (2) Cultivation conditions

[0520] Cultivation was carried out in a plastic greenhouse. Irrigation was carried out appropriately when the soil was dry. On days 106 and 126 after sowing, 0.4g of NK fertilizer (N:P:K = 15:0:15) was applied per pot at a ratio of N:P:K = 3:0:3 (kg / 10a). Additionally, for pest control, 3mL of Tilt emulsion 25 (Syngenta Japan KK) diluted 1000 times was sprayed per pot on day 113 after sowing, and SILVACUR Flowable (Bayer CropScience KK) diluted 2000 times was sprayed on day 132 after sowing, at a rate of 150L / 10a.

[0521] (3) Foliar spraying treatment

[0522] Prepare the following spray solution and apply it to the leaves using a spray gun (rechargeable Airbrush ver. 2.5, 0.4mm nozzle, Aurochs Ltd.) on day 133 after sowing. Foliar spraying was performed only once in two experimental areas. 2 mL was sprayed per pot.

[0523] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The evaluation test areas are shown below.

[0524] <Spray liquid>

[0525] Zone 1: Control (tap water only)

[0526] Zone 2: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 100 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0527] (4) Results

[0528] Harvesting was carried out on the 180th day after sowing. After dehulling, the wheat was dried at 90°C for 48 hours, and the grain weight of 4 plants per pot was measured. The grain weight results are expressed as follows: Figure 24 The bar chart in the figure represents the mean ± standard deviation. It shows that in wheat, increased yield can be achieved by applying a combination of ascorbic acid supplemented with components (B), (C), and (D). Compared to test area 1, in test area 2, the average grain weight per 4 wheat plants increased by approximately 2.2%.

[0529] Example 25 Effect of ascorbic acid composition on the growth of Komatsuna (Japanese mustard greens)

[0530] (1) Soil preparation

[0531] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and Komatsuna seeds (variety: Misugi, SakataSeed Corporation) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0532] (2) Cultivation conditions

[0533] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0534] (3) Foliar spraying treatment

[0535] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 27th day after sowing. Foliar spraying was performed only once in eight experimental areas. 6.7 mL was sprayed per plant.

[0536] The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. The test areas 1 to 8 for evaluation are shown below.

[0537] <Spray liquid>

[0538] Zone 1: Control (tap water only)

[0539] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0540] • Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 0.001 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0541] • Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 0.01 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0542] Zone 5: Foliar spray with ascorbic acid 1,500 ppm + BHT 0.1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0543] Zone 6: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0544] Zone 7: Foliar spray with ascorbic acid 1,500 ppm + BHT 5 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0545] Zone 8: Foliar spray with ascorbic acid 1,500 ppm + BHT 10 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0546] (4) Results

[0547] On day 34 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground dry weight measurements are expressed as follows: Figure 25 The bar chart in the figure represents the mean ± standard deviation. In Komatsuna, the comparison between experimental zones 2 and 3–8 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to experimental zone 1, the average increase in fresh aboveground weight was approximately 32.3% in experimental zone 2, approximately 87.1% in experimental zone 3, approximately 81.1% in experimental zone 4, approximately 37.4% in experimental zone 5, approximately 56.7% in experimental zone 6, approximately 71.2% in experimental zone 7, and approximately 70.8% in experimental zone 8. These results indicate that even when the concentration of BHT in component (B) is set to 0.001 ppm to 10 ppm, adding components (B), (C), and (D) to ascorbic acid can achieve a higher growth-promoting effect compared to spraying ascorbic acid dissolved in tap water alone.

[0548] Example 26 Effect of ascorbic acid composition on the growth of Komatsuna (Japanese mustard greens)

[0549] (1) Soil preparation

[0550] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and Komatsuna seeds (variety: Misugi, SakataSeed Corporation) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0551] (2) Cultivation conditions

[0552] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0553] (3) Foliar spraying treatment

[0554] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on the 25th day after sowing. Foliar spraying was performed only once in six experimental areas. 6.7 mL was sprayed per plant.

[0555] The spray solution was prepared by dissolving BHT from component (B) in component (D) which was preheated to 60°C, and then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used, including glycerin (manufactured by Fujifilm and Hikari Pure Chemical Industries Co., Ltd.), diethylene glycol (manufactured by Fujifilm and Hikari Pure Chemical Industries Co., Ltd.), and propylene glycol (manufactured by Fujifilm and Hikari Pure Chemical Industries Co., Ltd.). The test areas 1 to 6 for evaluation are shown below.

[0556] <Spray liquid>

[0557] Zone 1: Control (tap water only)

[0558] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0559] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0560] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + glycerin 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0561] Zone 5: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + diethylene glycol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0562] Zone 6: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + propylene glycol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0563] (4) Results

[0564] On day 32 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground dry weight measurements are expressed as follows: Figure 26 The bar chart in the figure represents the mean ± standard deviation. In Komatsuna, the comparison between experimental zones 2 and 3–6 shows that adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental zone 1, the average increase in fresh aboveground weight was approximately 8.4% in experimental zone 2, approximately 21.9% in experimental zone 3, 19.0% in experimental zone 4, 18.7% in experimental zone 5, and 22.4% in experimental zone 6. These results indicate that even when component (C) uses glycerol, diethylene glycol, or propylene glycol, adding components (B), (C), and (D) to ascorbic acid results in a higher growth-promoting effect compared to spraying ascorbic acid dissolved in tap water alone.

[0565] Example 27 Storage stability of single-dose formulations

[0566] (1) Preparation of single-dosage composition

[0567] A single-dose composition was prepared using 300g of ascorbic acid as component (A), 0.2g of BHT as component (B), 10mL of isobutanol as component (C), and 70mL of sorbitan monolaurate as component (D) by the following method: Component (B) was dissolved in component (C), then component (D) was mixed, and the resulting mixture was mixed with component (A) to prepare the single-dose composition. The reagents were the same as in Example 1.

[0568] (2) Preparation of powdered composition

[0569] As component (F), the carrier shown below is used to mix with the single-dose composition to prepare a powder composition. The content of each component in the resulting powder composition is shown in Table 10.

[0570] Zeolite (ZEOBUILDER CO., LTD), silica (Oriental Silicas Corporation), bentonite (Kurosaki Shirato Kogyo Co., Ltd.), and mirabilite (China-Salt Huaian Hongyun Salt Chemical Co., Ltd., with an average particle size of 25 μm after dry grinding of anhydrous mirabilite).

[0571] (3) Storage stability test

[0572] After storing the prepared powdered compositions at room temperature for one day, the compositions were then stacked on trays with two sieves (2.0 mm and 9.5 mm mesh) in sequence, starting with the sieve with the smallest mesh. 30 g of the composition was added to the top 9.5 mm sieve, the tray was covered, and the assembly was placed in a mini sieving machine (AS ONE Corporation, vibration speed 3). After vibrating for 10 minutes, the mass of the particles remaining on the sieves and trays was measured. The sieve passing rate was calculated by dividing the mass passing through the 2.0 mm sieve by the total mass.

[0573] Screening pass rate = (mass passing through the screen / total mass) × 100

[0574] The results are shown in Table 1. According to the results in Table 1, each powder composition has a higher sieve permeability compared with the comparative example, that is, the large particle size caused by surface adhesion during storage is suppressed, and therefore, the storage stability is excellent.

[0575] (4) Browning prevention test

[0576] Dispense 5g of each of the prepared powdered compositions into transparent glass bottles. Store in a storage room at a set temperature of 50°C for 2 weeks. After 2 weeks, evaluate the degree of browning appearance using four levels: (0: no browning, 1: slight browning, 2: browning, 3: obvious browning).

[0577] The results are shown in Table 1. As can be seen from Table 1, the composition with added silica suppressed browning after storage. On the other hand, other compositions showed browning of the appearance. The results indicate that, from the viewpoint of appearance stability, the addition of silica is excellent.

[0578] [Table 1]

[0579]

[0580] Example 28 Effect of ascorbic acid composition on the growth of Komatsuna (Japanese mustard greens)

[0581] (1) Soil preparation

[0582] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and Komatsuna seeds (variety: Misugi, SakataSeed Corporation) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0583] (2) Cultivation conditions

[0584] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0585] (3) Foliar spraying treatment

[0586] The following spray solution was prepared and applied to the leaves using a sprayer on the 21st day after sowing. Foliar spraying was performed only once in seven experimental areas. 6.7 mL was sprayed per plant. In experimental area 3, the spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water used, and then agent 2 was dissolved to prepare the spray solution.

[0587] The spray solutions in test areas 4-7 were prepared by the following procedure. First, BHT of component (B) was premixed with isobutanol, glycerol, diethylene glycol, or propylene glycol of component (C), and then sorbitan monolaurate of component (D) was added and mixed at 60°C. Next, ascorbic acid of component (A) and silica of component (F) were added and mixed to prepare a powdered formulation. This formulation was then dispersed in tap water to prepare the spray solution. The same reagents as in Examples 26 and 27 were used. Test areas 1-7 were evaluated as shown below.

[0588] <Spray liquid>

[0589] Zone 1: Control (tap water only)

[0590] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0591] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0592] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm + silica 300 ppm (dissolve in tap water).

[0593] Zone 5: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + glycerin 50 ppm + sorbitan monolaurate 350 ppm + silica 300 ppm (dissolve in tap water).

[0594] Zone 6: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + diethylene glycol 50 ppm + sorbitan monolaurate 350 ppm + silica 300 ppm (dissolve in tap water).

[0595] • Zone 7: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + propylene glycol 50 ppm + sorbitan monolaurate 350 ppm + silica 300 ppm (dissolve in tap water).

[0596] (4) Results

[0597] On day 28 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground dry weight measurements are expressed as follows: Figure 27The bar chart in the figure represents the mean ± standard deviation. In Komatsuna, the comparison between test areas 2 and 3–7 shows that adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to test area 1, the average increase in fresh aboveground weight was approximately 8.6% in test area 2, approximately 17.3% in test area 3, 31.9% in test area 4, 17.3% in test area 5, 36.4% in test area 6, and 23.5% in test area 7. These results indicate that even when component (C) uses glycerol, diethylene glycol, or propylene glycol, adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect compared to spraying ascorbic acid dissolved in tap water alone. Furthermore, it is shown that further addition of silica (component (F)) also results in a growth-promoting effect.

[0598] Example 29 Effect of ascorbic acid composition on the growth of Komatsuna (Japanese mustard greens)

[0599] (1) Soil preparation

[0600] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and Komatsuna seeds (variety: Misugi, SakataSeed Corporation) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0601] (2) Cultivation conditions

[0602] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0603] (3) Foliar spraying treatment

[0604] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on day 21 after sowing. Foliar spraying was performed only once in seven experimental areas. 6.7 mL was sprayed per plant.

[0605] The spray solution in test area 3 was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution.

[0606] The spray solutions in test areas 4-7 were prepared by the following steps. First, BHT of component (B) was premixed with isobutanol, glycerol, diethylene glycol, or propylene glycol of component (C), and then sorbitan monolaurate of component (D) was added and mixed at 60°C. Then, ascorbic acid and silica of component (A) were added and mixed to prepare a powdered formulation. This formulation was then dispersed in tap water to prepare the spray solution. The same reagents and sodium lauryl sulfate and sodium β-naphthalenesulfonic acid formaldehyde condensate were used as in Example 28. Test areas 1-7 were evaluated as shown below.

[0607] <Spray liquid>

[0608] Zone 1: Control (tap water only)

[0609] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0610] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0611] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + glycerin 50 ppm + sorbitan monolaurate 350 ppm + silica 300 ppm (dissolve in tap water).

[0612] Zone 5: Ascorbic acid 1,500 ppm + BHT 1 ppm + Glycerin 50 ppm + Sorbitan monolaurate 350 ppm + Silica 300 ppm + Triethanolamine lauryl sulfate 100 ppm. Foliar spray (dissolve in tap water).

[0613] Zone 6: Ascorbic acid 1,500 ppm + BHT 1 ppm + diethylene glycol 50 ppm + sorbitan monolaurate 350 ppm + silica 300 ppm + sodium β-naphthalenesulfonic acid formaldehyde condensate 100 ppm. Foliar spray (dissolve in tap water).

[0614] • Zone 7: Foliar spray with ascorbic acid 15,000 ppm + BHT 10 ppm + glycerin 500 ppm + sorbitan monolaurate 3,500 ppm + silica 3,000 ppm (dissolve in tap water).

[0615] (4) Results

[0616] On day 28 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground dry weight measurements are expressed as follows: Figure 28 The bar chart in the figure represents the mean ± standard deviation. In Komatsuna, the comparison between test areas 2 and 3–7 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than using ascorbic acid alone. Compared to test area 1, the average increase in fresh aboveground weight was approximately 15.3% in test area 2, approximately 33.7% in test area 3, approximately 36.0% in test area 4, approximately 52.4% in test area 5, approximately 53.0% in test area 6, and approximately 35.0% in test area 7. These results indicate that growth-promoting effects can also be achieved when component (D) is used in addition to sorbitan monolaurate, along with triethanolamine lauryl sulfate and sodium β-naphthalenesulfonic acid formaldehyde condensate. Furthermore, it is shown that high concentrations and low dosages of silica spraying also resulted in growth-promoting effects.

[0617] Example 30 Effect of ascorbic acid composition on the growth of Komatsuna (Japanese amaranth)

[0618] (1) Soil preparation

[0619] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and Komatsuna seeds (variety: Misugi, SakataSeed Corporation) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The number of replicates for each experimental area was set to 6 (n = 6).

[0620] (2) Cultivation conditions

[0621] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0622] (3) Foliar spraying treatment

[0623] The following spray solution was prepared and applied to the leaves using a sprayer on the 19th day after sowing. Foliar spraying was performed only once in four experimental areas. 6.7 mL was sprayed per plant. The spray solution was prepared by mixing components (B), (C), and (D), and further mixing component (E) in experimental area 4 to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used, including tocopherol (±α-tocopherol, manufactured by Fujifilm and Kojun Pharmaceutical Co., Ltd.), sodium sulfite (manufactured by Fujifilm and Kojun Pharmaceutical Co., Ltd.), glutathione (glutathione (reduced form), manufactured by Fujifilm and Kojun Pharmaceutical Co., Ltd.), uric acid (manufactured by Fujifilm and Kojun Pharmaceutical Co., Ltd.), and disodium ethylenediaminetetraacetate (2NA (EDTA·2Na), manufactured by Dojin Chemical Research Institute Co., Ltd.). The test areas 1 to 4 for evaluation are shown below.

[0624] <Spray liquid>

[0625] Zone 1: Control (tap water only)

[0626] Zone 2: Foliar spray with 500 ppm ascorbic acid (dissolved in tap water).

[0627] Zone 3: Foliar spray with ascorbic acid 500 ppm + tocopherol 10 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm + sodium sulfite 1 ppm + glutathione 100 ppm + uric acid 100 ppm (dissolve in tap water).

[0628] Zone 4: Foliar spray with ascorbic acid 500 ppm + tocopherol 10 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm + sodium sulfite 1 ppm + glutathione 100 ppm + uric acid 100 ppm + disodium EDTA 5 ppm

[0629] (4) Results

[0630] On day 26 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground dry weight measurements are expressed as follows: Figure 29The bar chart in the figure represents the mean ± standard deviation. In Komatsuna, the comparison between experimental zones 2 and 3 / 4 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than using ascorbic acid alone. Furthermore, adding components (B), (C), (D), and (E) to ascorbic acid also resulted in a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental zone 1, the average increase in fresh aboveground weight was approximately 9.1% in experimental zone 2, approximately 22.4% in experimental zone 3, and approximately 36.4% in experimental zone 4. The results from experimental zone 3 indicate that using sodium sulfite, glutathione, and uric acid as component (B) in addition to tocopherol resulted in a higher growth-promoting effect compared to spraying ascorbic acid dissolved in tap water alone. Furthermore, the results from test area 4 indicate that when disodium ethylenediaminetetraacetate (EDTA) is used as component (E), a higher growth-promoting effect can be obtained compared to spraying ascorbic acid dissolved in tap water alone.

[0631] Example 31 Effect of ascorbic acid composition on maize yield

[0632] (1) Cultivation conditions

[0633] Cultivation was carried out on a farm in Ibaraki Prefecture. AG Max (N:P:K = 10:10:10, i-Agri Corp.) was applied as a base fertilizer at the farm in a ratio of N:P:K = 20:20:20 (kg / 10a). Ten days after the base fertilizer application, corn seedlings (variety: SK4-117, Sakata Seed Corporation) that had grown in cell trays for 15 days were transplanted in two rows with a row spacing of 1.8m, a plant spacing of 0.3m, and a row spacing of 0.45m. Top dressing was applied on days 18 and 35 after transplanting, respectively, using NK-based fertilizer (N:P:K = 15:0:15) in a ratio of N:P:K = 5:0:5 (kg / 10a). In addition, for pest control, Benevia OD (FMC Chemicals Japan) diluted 2000 times was sprayed at a rate of 200 L / 10a on the 29th and 52nd days after transplanting. On the 42nd day after transplanting, Prebason Flowable 5 (FMC Chemicals Japan) and Alvaline granular water-soluble solution (Agro-Kanesho Co., Ltd.) diluted 2000 times were sprayed at a rate of 200 L / 10a respectively.

[0634] (2) Foliar spraying treatment

[0635] On day 37 post-transplanting, foliar spraying was performed using a battery-powered sprayer (ADB150Li, Maruyama Corporation). Foliar spraying was performed only once, and three experimental plots, including a control without treatment, were studied. Each experimental plot consisted of a 2.4m × 1.8m area with 16 corn plants, and the spray solution was applied at a rate of 100 L / 10a. Furthermore, the number of replicates for each experimental plot was set to 3 (n = 3).

[0636] The spray solution in test area 3 was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same types of reagents as in Example 1 were used. The test areas for evaluation are shown below.

[0637] <Spray liquid>

[0638] • Zone 1: Control (No treatment)

[0639] Zone 2: Ascorbic acid 1,500 ppm (dissolved in well water)

[0640] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolved in well water).

[0641] (3) Results

[0642] The first ear of corn was harvested on day 52 after transplanting, and the second ear was harvested on day 57 after transplanting. The weights of the first and second ears of corn were measured. The combined yield of the first and second ears per plant was expressed as follows: Figure 30 The bar chart in the figure represents the mean ± standard deviation. In maize, the comparison between test areas 2 and 3 shows that applying a combination of ascorbic acid with added components (B), (C), and (D) resulted in a higher yield increase than when ascorbic acid was used alone. Compared to test area 1, the average yield per maize plant increased by approximately 0.2% in test area 2 and by approximately 5% in test area 3.

[0643] Example 32 Effects of the ascorbic acid composition on the growth of melon

[0644] (1) Soil preparation

[0645] Mid-term fertility-enhancing soil (TAKII water-containing cellular soil mid-term fertility type, TAKII Seedling Co., Ltd.) and fine vermiculite (AKAGI ENGEI CO., LTD.) were mixed at a volume ratio of 1:1, and the mixture was filled into plastic seedling trays (10.5 cm in diameter and 9 cm in height). After supplying each tray with 250 mL of tap water, one melon seed (variety: Earl's Royal Summer series, Kanda Breeding Farm Co., Ltd.) was sown in each tray at a depth of approximately 1 cm from the soil surface. Furthermore, the number of replicates for each experimental area was set to 6 (n = 6).

[0646] (2) Cultivation conditions

[0647] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0648] (3) Foliar spraying treatment

[0649] The following spray solution was prepared and applied to the leaves using a sprayer on the 22nd day after sowing. Foliar spraying was performed only once in three test areas. 6.7 mL was sprayed per plant. The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. Test areas 1-3 were evaluated as described below.

[0650] <Spray liquid>

[0651] Zone 1: Control (tap water only)

[0652] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0653] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0654] (4) Results

[0655] On day 29 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground fresh weight measurement are expressed as follows: Figure 31The bar chart in the figure represents the mean ± standard deviation. In melons, a comparison between experimental zones 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid yields a greater growth-promoting effect than using ascorbic acid alone. Compared to experimental zone 1, the average fresh weight of the aboveground parts increased by approximately 0.5% in experimental zone 2 and by approximately 17.7% in experimental zone 3.

[0656] Example 33 Effect of ascorbic acid composition on okra growth

[0657] (1) Soil preparation

[0658] Mid-term fertility potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (AKAGI ENGEI CO., LTD.) were mixed at a volume ratio of 1:1 and filled into plastic seedling trays (10.5 cm in diameter and 9 cm in height). After supplying each tray with 250 mL of tap water, four okra seeds (variety: Peak Five, SakataSeed Corporation) were sown, one seed at a depth of approximately 5 mm from the soil surface. After germination, thinning was performed so that each tray contained one plant. The replicate number for each experimental area was set to 6 (n = 6).

[0659] (2) Cultivation conditions

[0660] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0661] (3) Foliar spraying treatment

[0662] The following spray solution was prepared and applied to the leaves using a sprayer on the 22nd day after sowing. Foliar spraying was performed only once in three test areas. 6.7 mL was sprayed per plant. The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. Test areas 1-3 were evaluated as described below.

[0663] <Spray liquid>

[0664] Zone 1: Control (tap water only)

[0665] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0666] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0667] (4) Results

[0668] On day 29 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground fresh weight measurement are expressed as follows: Figure 32 The bar chart in the figure represents the mean ± standard deviation. In okra, the comparison between experimental zones 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than when ascorbic acid was used alone. Compared to experimental zone 1, the average fresh weight of the aboveground parts increased by approximately 5.3% in experimental zone 2 and by approximately 14.2% in experimental zone 3.

[0669] Example 34 Effect of ascorbic acid composition on the growth of garland chrysanthemum

[0670] (1) Soil preparation

[0671] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). Each tray was supplied with 250 mL of tap water, and garland chrysanthemum seeds (Atariya Agricultural Co., Ltd.) were sown. Four seeds were sown in each tray, one seed at a depth of approximately 5 mm from the soil surface. After germination, seedlings were thinned to one plant per tray. The replicates for each experimental area were set to 6 (n = 6).

[0672] (2) Cultivation conditions

[0673] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0674] (3) Foliar spraying treatment

[0675] The following spray solution was prepared and applied to the leaves using a sprayer on the 25th day after sowing. Foliar spraying was performed only once in six test areas. 6.7 mL was sprayed per plant. The spray solution was prepared by dissolving BHT from component (B) in component (D) preheated to 60°C, then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 29 were used. Test areas 1-6 were evaluated as shown below.

[0676] <Spray liquid>

[0677] Zone 1: Control (tap water only)

[0678] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0679] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 10 ppm + sorbitan monolaurate 100 ppm (dissolve in tap water).

[0680] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0681] Zone 5: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + triethanolamine lauryl sulfate 350 ppm (dissolve in tap water).

[0682] Zone 6: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sodium salt of β-naphthalenesulfonic acid formaldehyde condensate 350 ppm (dissolve in tap water).

[0683] (4) Results

[0684] On day 32 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground dry weight measurements are expressed as follows: Figure 33The bar chart in the figure represents the mean ± standard deviation. In *Chrysanthemum indicum*, the comparison between experimental zones 2 and 3–6 shows that adding components (B), (C), and (D) to ascorbic acid yields a higher growth-promoting effect than using ascorbic acid alone. Compared to experimental zone 1, the average increase in fresh aboveground weight was approximately 13.4% in experimental zone 2, approximately 29.2% in experimental zone 3, 41.6% in experimental zone 4, 23.3% in experimental zone 5, and 29.6% in experimental zone 6. These results indicate that, when component (D) is triethanolamine lauryl sulfate and sodium formaldehyde condensate β-naphthalenesulfonic acid, adding components (B), (C), and (D) to ascorbic acid also yields a higher growth-promoting effect than spraying ascorbic acid dissolved in tap water alone.

[0685] Example 35 Effects of the ascorbic acid composition on the growth of pumpkin

[0686] (1) Soil preparation

[0687] Mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) and fine vermiculite (AKAGI ENGEI CO., LTD.) were mixed at a volume ratio of 1:1 and filled into plastic seedling trays (10.5 cm in diameter and 9 cm in height). After supplying each tray with 250 mL of tap water, two pumpkin seeds (variety: Yunlong No. 1 Improved R type, Kurume Original Seed Breeding Association Co., Ltd.) were sown in each tray at a depth of about 1 cm from the soil surface. After germination, the seedlings were thinned to one plant per tray. The replicate number for each experimental area was set to 6 (n = 6).

[0688] (2) Cultivation conditions

[0689] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0690] (3) Foliar spraying treatment

[0691] The following spray solution was prepared and applied to the leaves using a sprayer on the 19th day after sowing. Foliar spraying was performed only once in three test areas. 6.7 mL was sprayed per plant. The spray solution was prepared by first dissolving BHT in component (B) in component (C), then mixing component (C) with component (D) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the working water, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 1 were used. Test areas 1-3 were evaluated as described below.

[0692] <Spray liquid>

[0693] Zone 1: Control (tap water only)

[0694] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0695] Zone 3: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + isobutanol 50 ppm + sorbitan monolaurate 350 ppm (dissolve in tap water).

[0696] (4) Results

[0697] On day 25 after sowing, the fresh weight of the aboveground parts of the plant was measured. The results of the aboveground fresh weight measurement are expressed as follows: Figure 34 The bar chart in the figure represents the mean ± standard deviation. In pumpkin, the comparison between test areas 2 and 3 shows that adding components (B), (C), and (D) to ascorbic acid resulted in a higher growth-promoting effect than using ascorbic acid alone. Compared to test area 1, the average fresh weight of the aboveground parts increased by approximately 0.7% in test area 2 and by approximately 4.4% in test area 3.

[0698] Example 36: Effects of the ascorbic acid composition on the growth of radishes

[0699] (1) Soil preparation

[0700] A 1:1 volume ratio of mid-term fertility-enhancing potting mix (TAKII water-containing cellular potting mix, TAKII Seedling Co., Ltd.) to fine vermiculite (AKAGI ENGEI CO., LTD.) was used to fill plastic seedling trays (10.5 cm in diameter, 9 cm in height). After supplying 250 mL of tap water to each tray, small radish seeds (variety: Isabel, KanekoSeeds Co., Ltd.) were sown. Four seeds were sown one at a depth of approximately 1 cm from the soil surface in each tray. After germination, thinning was carried out so that each tray contained one plant. The number of replicates for each experimental area was set to 6 (n = 6).

[0701] (2) Cultivation conditions

[0702] Cultivation was carried out indoors, with cultivation conditions set as follows: 16 hours of light, 25°C, LED light source, and light intensity of 400–440 μmol / m³. 2 / s. Irrigation is carried out by adding tap water to the saucer placed under the basin after the water has evaporated, enough to submerge the bottom 5cm of the basin.

[0703] (3) Foliar spraying treatment

[0704] Prepare the spray solution shown below and apply it to the leaves using a spray bottle on day 19 after sowing. Foliar spraying was performed only once in six experimental areas. 6.7 mL was sprayed per plant.

[0705] The spray solution was prepared by first dissolving component (B) in component (C) to prepare agent 1. Component (A) was used as agent 2. Agent 1 was dissolved in the water to be used, and then agent 2 was dissolved to prepare the spray solution. The same reagents as in Example 30, including sodium ascorbate (manufactured by Fujifilm and Kohden Chemical Co., Ltd.) and potassium sulfite (manufactured by Fujifilm and Kohden Chemical Co., Ltd.), were used. The evaluation test areas 1 to 6 are shown below.

[0706] <Spray liquid>

[0707] Zone 1: Control (tap water only)

[0708] • Zone 2: Foliar spray with ascorbic acid 1,500 ppm (dissolved in tap water)

[0709] Zone 3: Foliar spray with 1,500 ppm ascorbic acid + 1 ppm BHT + 50 ppm isobutanol (dissolve in tap water).

[0710] Zone 4: Foliar spray with ascorbic acid 1,500 ppm + BHT 1 ppm + dimethyl sulfoxide 50 ppm (dissolve in tap water).

[0711] Zone 5: Foliar spray with sodium ascorbate 1,500 ppm + tocopherol 10 ppm + isobutanol 50 ppm (dissolve in tap water).

[0712] Zone 6: Foliar spray with sodium ascorbate 1,500 ppm + tocopherol 10 ppm + isobutanol 50 ppm + sodium sulfite 1 ppm + potassium sulfite 1 ppm + glutathione 100 ppm + uric acid 100 ppm (dissolve in tap water).

[0713] (4) Results

[0714] On day 25 after sowing, the fresh weight of the underground parts of the plant was measured. The results of the fresh weight measurement of the underground parts are expressed as follows: Figure 35 The bar chart in the figure represents the mean ± standard deviation. In radishes, the comparison between experimental zones 2 and 3–6 shows that ascorbic acid alone did not confirm a growth-promoting effect, but growth-promoting effects were obtained by adding components (B) and (C) to ascorbic acid or sodium ascorbate. Compared to experimental zone 1, the average fresh weight of the underground parts increased by approximately 3.3% in experimental zone 3, approximately 17.6% in experimental zone 4, approximately 13.9% in experimental zone 5, and approximately 21.8% in experimental zone 6.

Claims

1. A plant growth promoter selected from fruits and vegetables, leafy and stem vegetables, root vegetables, and grains, wherein, The agent is a combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent. (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of (A) ascorbic acid or its salt is 10 to 3,000,000 by mass.

2. A plant growth promoter selected from fruits and vegetables, leafy and stem vegetables, root vegetables, and grains, wherein, The agent is a combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent. (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of organic solvent (C) is 4 to 100,000 by mass.

3. A yield-enhancing agent selected from plants including fruits and vegetables, leafy and stem vegetables, root vegetables, and grains, wherein, The agent is a combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent. (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of (A) ascorbic acid or its salt is 10 to 3,000,000 by mass.

4. A yield-enhancing agent selected from plants including fruits and vegetables, leafy and stem vegetables, root vegetables, and grains, wherein, The agent is a combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent. (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of organic solvent (C) is 4 to 100,000 by mass.

5. The agent according to any one of claims 1 to 4, wherein, The ratio of (A) ascorbic acid or its salt and (C) organic solvent, by mass ratio, is 10 to 3,000,000 for component (B) and 4 to 100,000 for component (C) when component (B) is set to 1.

6. The agent according to any one of claims 1 to 5, wherein, (B) The antioxidants also include one or more of the following: sodium sulfite, potassium sulfite, glutathione and uric acid.

7. The agent according to any one of claims 1 to 6, wherein, (C) The organic solvent is selected from one or more of isobutanol, dimethyl sulfoxide and glycerol.

8. The agent according to any one of claims 1 to 7, wherein, It is also composed of (D) surfactants.

9. The agent as claimed in claim 8, wherein, When component (B) is set to 1, the proportion of surfactant (D) is 10 to 300,000 by mass.

10. The agent as described in claim 8 or 9, wherein, (D) Surfactants include at least nonionic surfactants.

11. The agent as claimed in claim 10, wherein, (D) The nonionic surfactant is selected from one or more of the following: sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters and polyoxyethylene alkyl ethers.

12. The agent as claimed in claim 10, wherein, (D) Surfactants also include anionic surfactants or amphoteric surfactants.

13. The agent as claimed in claim 12, wherein, (D) The nonionic surfactant is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, and polyoxyethylene alkyl ethers. The anionic surfactant is selected from one or more of fatty acid salts, aromatic sulfonate formaldehyde condensates, and alkyl sulfates. The amphoteric surfactant is 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazoline betaine.

14. The agent according to any one of claims 1 to 13, wherein, It is also composed of (E) chelating agents.

15. The agent as claimed in claim 14, wherein, The proportions of (A) ascorbic acid or its salt, (C) organic solvent, (D) surfactant and (E) chelating agent, by mass ratio, are as follows: when component (B) is set to 1, component (A) is 10 to 3,000,000, component (C) is 4 to 100,000, component (D) is 10 to 300,000 and component (E) is 0.01 to 100.

16. The agent as described in claim 14 or 15, wherein, (E) The chelating agent is selected from one or more of ethylenediaminetetraacetic acid or its salt and ethylenediamine-N,N'-disuccinic acid.

17. The agent according to any one of claims 1 to 16, wherein, It is also combined with (F) carriers.

18. The agent as claimed in claim 17, wherein, When component (B) is set to 1, the proportion of carrier (F) is 50 to 1,000 by mass.

19. The agent as claimed in claim 17 or 18, wherein, (F) The carrier is selected from one or more of zeolite, silica, bentonite and mirabilite.

20. The agent according to any one of claims 1 to 7, wherein, It comprises a first agent containing said component (A) and a second agent containing said components (B) and (C). Combine the two when using them.

21. A method for promoting the growth of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables, and grains, wherein, The method includes the step of applying a combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent with water to soil or plants. (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of (A) ascorbic acid or its salt is 10 to 3,000,000 by mass.

22. A method for increasing the yield of plants selected from fruits and vegetables, leafy and stem vegetables, root vegetables, and grains, wherein, The method includes the step of applying a combination of (A) ascorbic acid or its salt, (B) an antioxidant, and (C) an organic solvent with water to soil or plants. (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of (A) ascorbic acid or its salt is 10 to 3,000,000 by mass.

23. The method of claim 21 or 22, wherein, Prepare a first agent containing ingredient (A) and a second agent containing ingredients (B) and (C), and apply them together to the soil or plants when in use.

24. The agent as claimed in claim 20, wherein, When the second agent is set to 1, the weight ratio of the first agent is 1 to 100.

25. The method of claim 23, wherein, Dissolve the first and second agents in water before use.

26. The method of claim 25, wherein, The concentration of the first agent in the aqueous solution is between 100 ppm and 300,000 ppm by mass, and the concentration of the second agent is between 10 ppm and 100,000 ppm by mass.

27. Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant, and (C) an organic solvent in the manufacture of a plant growth promoter selected from fruits, vegetables, leafy greens, root vegetables, and cereals, wherein, (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of (A) ascorbic acid or its salt is 10 to 3,000,000 by mass.

28. Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant, and (C) an organic solvent in the manufacture of a plant growth promoter selected from fruits, vegetables, leafy greens, root vegetables, and cereals, wherein, (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of organic solvent (C) is 4 to 100,000 by mass.

29. Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant, and (C) an organic solvent in the manufacture of a yield enhancer for plants selected from fruits, vegetables, leafy greens, root vegetables, and cereals, wherein, (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of (A) ascorbic acid or its salt is 10 to 3,000,000 by mass.

30. Use of a combination of (A) ascorbic acid or a salt thereof, (B) an antioxidant, and (C) an organic solvent in the manufacture of a yield enhancer for plants selected from fruits, vegetables, leafy greens, root vegetables, and cereals, wherein, (B) The antioxidant is at least one selected from tocopherol, butylated hydroxytoluene, and butylated hydroxyanisole. (C) The organic solvent is one or more selected from alcohols with 5 or fewer carbon atoms, dimethyl sulfoxide, and polyols. When component (B) is set to 1, the proportion of organic solvent (C) is 4 to 100,000 by mass.

31. The use as described in any one of claims 27 to 30, wherein, The ratio of (A) ascorbic acid or its salt and (C) organic solvent, by mass ratio, is 10 to 3,000,000 for component (B) and 4 to 100,000 for component (C) when component (B) is set to 1.

32. The use as described in any one of claims 27 to 30, wherein, (B) The antioxidants also include one or more of the following: sodium sulfite, potassium sulfite, glutathione and uric acid.

33. The use as described in any one of claims 27 to 30, wherein, (C) The organic solvent is selected from one or more of isobutanol, dimethyl sulfoxide and glycerol.

34. The use as described in any one of claims 27 to 30, wherein, It is also composed of (D) surfactants.

35. The use as described in claim 34, wherein, When component (B) is set to 1, the proportion of surfactant (D) is 10 to 300,000 by mass.

36. The use as described in claim 34 or 35, wherein, (D) Surfactants include at least nonionic surfactants.

37. The use as described in claim 36, wherein, (D) The nonionic surfactant is selected from one or more of the following: sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, polyoxyethylene resin esters and polyoxyethylene alkyl ethers.

38. The use as described in claim 36, wherein, (D) Surfactants also include anionic surfactants or amphoteric surfactants.

39. The use as described in claim 38, wherein, (D) The nonionic surfactant is selected from one or more of sorbitan fatty acid esters, glycerol fatty acid esters, polyalkylene glycol fatty acid esters, and polyoxyethylene alkyl ethers. The anionic surfactant is selected from one or more of fatty acid salts, aromatic sulfonate formaldehyde condensates, and alkyl sulfates. The amphoteric surfactant is 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazoline betaine.

40. The use as described in any one of claims 27 to 39, wherein, It is also composed of (E) chelating agents.

41. The use as described in claim 40, wherein, The proportions of (A) ascorbic acid or its salt, (C) organic solvent, (D) surfactant and (E) chelating agent, by mass ratio, are as follows: when component (B) is set to 1, component (A) is 10 to 3,000,000, component (C) is 4 to 100,000, component (D) is 10 to 300,000 and component (E) is 0.01 to 100.

42. The use as described in claim 40 or 41, wherein, (E) The chelating agent is selected from one or more of ethylenediaminetetraacetic acid or its salt and ethylenediamine-N,N'-disuccinic acid.

43. The use as described in any one of claims 27 to 42, wherein, It is also combined with (F) carriers.

44. The use as described in claim 43, wherein, When component (B) is set to 1, the proportion of carrier (F) is 50 to 1,000 by mass.

45. The use as described in claim 43 or 44, wherein, (F) The carrier is selected from one or more of zeolite, silica, bentonite and mirabilite.

46. ​​The use as described in any one of claims 27 to 30, wherein, It comprises a first agent containing said component (A) and a second agent containing said components (B) and (C). Combine the two when using them.

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