Culture medium for breeding polygonatum kingianum tubers and breeding method of polygonatum kingianum tubers

Abstract

The invention belongs to the technical field of polygonatum rhizome breeding, and particularly relates to a polygonatum rhizome tuber breeding culture medium and a polygonatum rhizome tuber breeding method.The polygonatum rhizome tuber breeding culture medium is composed of vermiculite, a bactericide and water, the addition amount of the water meets the standard that the vermiculite fully absorbs the water and the vermiculite is clustered by hand without water, and the mass concentration of the bactericide is 0.1-0.2%; according to the method, vermiculite is adopted, only vermiculite is used as a culture medium for polygonatum rhizome tuber breeding, the vermiculite provides a relatively loose environment for polygonatum rhizome tubers, compared with a mixed culture medium of perlite, peat soil and decomposed crushed bark, the condition is possibly closer to the breeding condition of natural polygonatum rhizome tubers, and the survival rate and the new root germination rate are higher.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of propagation of Rhizoma Polygonati, and particularly relates to a culture medium for propagation of Rhizoma Polygonati and a propagation method of Rhizoma Polygonati. BACKGROUND

[0002] During the propagation of Rhizoma Polygonati, some specific culture medium formulations are required in different culture stages, which can be mainly divided into induction, proliferation and rooting, and the specific formulations are as follows: I. Induction medium for adventitious bud ‌MS+6-BA 1.0~4.0 mg / L+NAA 0.1~0.5 mg / L, 0.7% agar + 3% sucrose, pH 5.8, used for primary culture of explants to induce differentiation of adventitious buds.

[0003] ‌MS+6-BA 2.0mg / L+2,4-D 0.2mg / L, suitable for induction of rhizome explant callus, and low-concentration 6-BA promotes callus formation.

[0004] II. Proliferation medium ‌MS+6-BA 1.0~4.0mg / L+NAA 0.1~1.5mg / L (or IAA), 0.7% agar + 3% sucrose, pH 5.8, used for subculture and proliferation of multiple shoots.

[0005] ‌MS+6-BA 6.0mg / L+NAA 0.2mg / L or MS+6-BA 4.0mg / L+TDZ 0.2mg / L+NAA 0.2mg / L, low-concentration TDZ (thidiazuron) can promote proliferation and growth of adventitious buds.

[0006] III. Rooting medium ‌1 / 2 MS+IBA 0.5~2.0mg / L (or NAA, IAA), 0.7% agar + 1.5% sucrose, dark culture for 7 days and then light culture, to promote root development.

[0007] ‌1 / 2 MS+IBA 1.0mg / L, high-concentration IBA may inhibit rooting, and this formulation has better rooting effect.

[0008] ‌1 / 2 MS+NAA 1.0mg / L+activated carbon 0.20g / L, activated carbon adsorbs harmful substances, and the rooting rate is increased to more than 90%.

[0009] IV. Auxiliary components and culture conditions ‌Basic components: sucrose (3%), agar (0.7%) or carrageenan (7g / L).

[0010] Patent application publication CN 109463238 A discloses a kind of cultivation method of polygonatum sibiricum root tuber, including the following steps, fresh polygonatum sibiricum stem is according to every two node stem, as a breeding stem, the sticky material secreted by wound is dried, then is soaked in treatment liquid 3-7min, the treatment liquid includes the following components: gibberellin, 6-BA, ethyl propylthiocarbamate and captan, then the treated stem and culture medium interval layer are laid, sealed, the humidity of culture medium is controlled to be 55-65%, temperature is 22-27 DEG C, the culture medium includes the following components: perlite, peat soil, rotten bark powder and treatment liquid. SUMMARY

[0011] The technical problem to be solved by the present application is to provide a culture medium for breeding polygonatum sibiricum root tuber and a breeding method of polygonatum sibiricum root tuber, which effectively improves the survival rate and new root germination rate.

[0012] The culture medium for breeding polygonatum sibiricum root tuber provided by the embodiment of the present application is composed of vermiculite, fungicide and water, the water is added in an amount sufficient for the vermiculite to absorb water and form a lump without water leakage when the vermiculite is pinched by hand, and the mass concentration of the fungicide is 0.1-0.2%.

[0013] Preferably, the fungicide is carbendazim.

[0014] Preferably, the particle size of the vermiculite is 1-3mm.

[0015] The breeding method of polygonatum sibiricum root tuber provided by the embodiment of the present application includes the following steps: polygonatum sibiricum rhizomes are broken into a plurality of tubers, the tubers with buds are immersed in water containing a fungicide, dried, then the dried polygonatum sibiricum tubers and the culture medium according to any one of claims 1-3 are alternately and sequentially stacked, the culture medium is placed in the bottom layer and the top layer, the thickness of the single-layer culture medium is 2-5mm, the alternately and sequentially stacked polygonatum sibiricum tubers and culture medium are placed in a sealing film, a through hole is formed in the bottom of the sealing film, and then stored in a constant-temperature environment.

[0016] Preferably, the fungicide is carbendazim and prochloraz.

[0017] Preferably, the mass concentration of carbendazim is 0.1-0.2%, and the mass concentration of prochloraz is 0.1-0.2%.

[0018] Preferably, the tubers with buds are immersed in water containing the fungicide for 10-15min.

[0019] Preferably, the length of the polygonatum sibiricum tuber is 0.5-1cm.

[0020] Preferably, in the constant-temperature environment, the temperature is 20-25 DEG C.

[0021] The present application has the beneficial effect that the present application uses vermiculite and only uses vermiculite as the culture medium for the propagation of polygonatum tubers, and the vermiculite provides a relatively loose environment for the polygonatum tubers, which is more close to the natural propagation conditions of the polygonatum tubers than the mixed culture medium of perlite, peat soil and decomposed crushed tree bark, and has a higher survival rate and new root germination rate. DETAILED DESCRIPTION

[0022] Example 1 A propagation method of polygonatum tubers, comprising the following steps: 1. First, select polygonatum rhizomes that are thick and free of diseases, and dig the rhizomes in the autumn and spring every year. Then, the polygonatum rhizomes are treated by breaking the rhizomes into small pieces of 0.5-1 cm, and selecting the pieces with and without bud points. The pieces with bud points are poured into water containing carbendazim and prochloraz, soaked for 10-15 minutes, and then taken out to dry. The mass concentration of carbendazim is 0.125%, and the mass concentration of prochloraz is 0.125%.

[0023] 2. Prepare a culture medium for the propagation of polygonatum tubers, which is composed of vermiculite, carbendazim and water. The amount of water added is determined by the fact that the vermiculite fully absorbs water and the vermiculite does not come out when it is kneaded by hand. The mass concentration of carbendazim is 0.125%.

[0024] 3. Cut the black PE film to the appropriate length and punch holes on one side of the bottom of the frame. Sprinkle 3-5 cm of the culture medium on the bottom of the frame, and evenly place 100 polygonatum tubers on each layer. Sprinkle 2-3 cm of the culture medium again, and place 100 polygonatum tubers again. Finally, sprinkle 3-5 cm of the culture medium on the top layer. Cover the top of the frame with the PE film for storage. Store in a closed constant temperature (20-25℃) environment. Ensure good sealing to prevent water evaporation and bacterial invasion. Check the humidity regularly to keep the vermiculite moist but not waterlogged, so as to promote the germination and growth of the polygonatum tubers.

[0025] Comparative Example 1 Comparative Example 1 uses the culture medium in Patent Application Publication No. CN 109463238 A, i.e. perlite, peat soil and decomposed crushed tree bark (weight ratio 1:1:1) instead of vermiculite in Example 1. That is, the culture medium for the propagation of polygonatum tubers in Comparative Example 1 is composed of perlite, peat soil, decomposed crushed tree bark (weight ratio 1:1:1), carbendazim and water. The amount of water added is determined by the fact that the perlite, peat soil and decomposed crushed tree bark fully absorb water and do not come out when they are kneaded by hand. The mass concentration of carbendazim is 0.125%. The other aspects are the same as in Example 1.

[0026] Comparative Example 2 Comparative Example 2 and Example 1 are different in that no carbendazim is added to the culture medium for the propagation of polygonatum tubers, and the other aspects are the same as in Example 1.

[0027] Comparative Example 3 Comparative Example 3 and Example 1 are different in that the small pieces of rhizomes with bud points are poured into water, soaked for 10-15 minutes, and then taken out to dry, and the rest is the same as Example 1.

[0028] The same batch of rhizomes with similar quality and size are used for cultivation, and the survival rate and new root germination rate of rhizomes obtained by different cultivation methods are tested, and the experimental data shown in Table 1 are obtained.

[0029] Table 1 Survival rate and new root germination rate of rhizomes obtained by different cultivation methods

[0030] From the data in Table 1, it can be seen that the rhizome cultivation using the culture medium of the present application has a significantly better survival rate and new root germination rate compared to other culture media.

[0031] It should be understood by those skilled in the art that the above discussion of any of the embodiments is only exemplary and is not intended to suggest that the scope of protection of the present application is limited to these examples; under the idea of the present application, the above embodiments or technical features in different embodiments can also be combined, the steps can be implemented in any order, and there are many other changes of different aspects of one or more embodiments of the present application as described above. In order to be brief, they are not provided in details.

[0032] One or more embodiments of the present application are intended to cover all such alternatives, modifications and variations falling within the broad scope of the present application. Therefore, any omissions, modifications, equivalent replacements, improvements, etc. made in the spirit and principles of one or more embodiments of the present application should be included in the scope of protection of the present application.

Claims

1. A culture medium for propagating Polygonatum rhizome tubers, characterized in that it consists of... The product consists of the following components: vermiculite, bactericide, and water. The amount of water added should be such that the vermiculite fully absorbs the water and can be formed into a clump without releasing water when squeezed by hand. The mass concentration of the bactericide is 0.1-0.2%.

2. The culture medium as described in claim 1, characterized in that, The bactericide is carbendazim.

3. The culture medium as described in claim 1, characterized in that, The vermiculite has a particle size of 1-3 mm.

4. A method for propagating Polygonatum rhizome tubers, characterized in that, The procedure includes the following steps: breaking the rhizome of Polygonatum into multiple tubers, immersing the tubers with buds in water containing a bactericidal substance, drying them, and then alternately layering the dried Polygonatum tubers and the culture medium as described in any one of claims 1-3, with the culture medium placed at the bottom and top layers, and the thickness of a single layer of culture medium being 2-5 mm. The alternately layered Polygonatum tubers and culture medium are placed in a sealed film with a through hole at the bottom, and then stored in a constant temperature environment.

5. The breeding method as described in claim 4, characterized in that, The bactericidal substances are carbendazim and imazalil.

6. The breeding method as described in claim 5, characterized in that, The mass concentration of carbendazim is 0.1-0.2%, and the mass concentration of prochloraz is 0.1-0.2%.

7. The breeding method as described in claim 4, characterized in that, Immerse the tubers with buds in water containing a bactericidal substance for 10-15 minutes.

8. The breeding method as described in claim 4, characterized in that, The length of the rhizome of Polygonatum is 0.5-1cm.

9. The breeding method as described in claim 4, characterized in that, In a constant temperature environment, the temperature is 20-25℃.

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