Breeding method of special rice variety for patients with kidney diseases and diabetes

Abstract

The invention belongs to the technical field of rice variety breeding, and particularly relates to a breeding method of a special rice variety for patients with kidney diseases and diabetes mellitus, which comprises the following specific steps: selecting a parent material: taking a low-water-soluble protein mutant variety LGC-1 introduced from a radiation breeding field of the Japanese biological resource research institute as a female parent; the Qingyou 308 rice variety bred by Institute of Rice Institute of Academy of Agricultural Sciences in Fujian Province is used as a male parent for hybridization; hybrid progenies are obtained through hybridization, selfing is carried out, progeny seeds are analyzed from F1, and seeds with low glutelin characters and high alcohol-soluble protein characters are screened out; selecting seeds with low glycemic index from the seeds with low glutelin character and high prolamin character; seeds with high amylose content are selected from the seeds with the low glycemic index. The breeding method has the advantages that the bred seeds can be suitable for kidney patients and also suitable for diabetics.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of breeding rice varieties, in particular to a method for breeding rice varieties for patients with kidney disease and diabetes. BACKGROUND

[0002] The protein metabolism disorder of patients with kidney disease and diabetes (with kidney disease) cannot eat rice with more than 4% of absorbable protein content during treatment, so a low-protein diet combined with treatment is an effective measure.

[0003] Although rice with a protein content of not more than 4% can be bred at present, it is generally more suitable for kidney patients, because in existing rice, the lower the protein content, the higher the glycemic index may be. Therefore, a method for breeding rice varieties for patients with kidney disease and diabetes is invented. SUMMARY

[0004] To solve the above technical problems, according to one aspect of the present application, the present application provides the following technical scheme:

[0005] A method for breeding rice varieties for patients with kidney disease and diabetes, comprising the following specific steps:

[0006] Step one, selecting parent materials: introducing a low water-soluble protein mutant variety LGC-1 from the Japan Biological Resources Institute Radiation Breeding Field as the female parent, and selecting Qingyou 308 rice variety from the Fujian Provincial Academy of Agricultural Sciences Rice Institute as the male parent, and hybridizing;

[0007] Step two: obtaining hybrid offspring by hybridization, and selfing, and analyzing the seeds of the offspring from F1 to screen out seeds with low glutelin traits and high prolamin traits;

[0008] Step three: selecting seeds with low glycemic index from seeds with low glutelin traits and high prolamin traits;

[0009] Step four: selecting seeds with high amylose from seeds with low glycemic index;

[0010] Step five: thus, rice seeds with low glutelin traits, high prolamin traits, low glycemic index and high amylose are obtained.

[0011] As a preferred scheme of the method for breeding rice varieties for patients with kidney disease and diabetes, in the step two, the seeds with low glutelin traits have a glutelin content of 3% to 4%.

[0012] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, in the step two, the high prolamine content of the seed is 60-65%.

[0013] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, in the step three, the low glycemic index of the seed is 45-50.

[0014] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, in the step four, the high amylose content of the seed is 30-40%.

[0015] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, in the step two, the seed is analyzed by sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis.

[0016] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, in the step three, the glycemic index of the seed is analyzed by an instrument.

[0017] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, the instrument includes a high-performance liquid chromatograph, a mass spectrometer, and an infrared spectrometer.

[0018] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, in the step three, the amylose content of the seed is analyzed by near-infrared analysis.

[0019] As a preferred method for breeding a rice variety for kidney disease and diabetes patients, the specific process of the near-infrared analysis is as follows:

[0020] Process one: obtain the amylose content reference value of the sample single grain by using "Determination of Amylose Content in Rice, Corn, and Millet Grains" (gb7648-87), establish a reference value matrix, and obtain the experimental standard deviation of the experimenter according to the national standard method.

[0021] Process two: pretreat the spectrum, select effective wavelengths, increase the signal-to-noise ratio, and eliminate interference.

[0022] Process three: extract the spectral characteristics, reduce the dimension of the data using PLS, and establish a spectral matrix.

[0023] Process four: the information of the spectral matrix and the information of the reference value matrix are regressed and correlated by using the PLSR method and the SVR method, and a model for detecting amylose in the single-seed grain of the crop is established;

[0024] Process five: after collecting the diffuse reflection spectrum of the sample, the abnormal spectrum is removed, and the original spectrum of the sample is obtained, at this time, the amylose in the seed can be analyzed by the model for detecting amylose in the single-seed grain of the crop.

[0025] Compared with the prior art, the present application has the following advantages:

[0026] By screening the seeds with low glutelin traits and high prolamin traits, selecting the seeds with low glycemic index in the seeds with low glutelin traits and high prolamin traits, and selecting the seeds with high amylose in the seeds with low glycemic index, the selected seeds can not only be suitable for kidney patients, but also be suitable for diabetic patients. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the present application more clear, the embodiments of the present application will be further described in detail.

[0028] Embodiment 1:

[0029] The present application provides a breeding method for rice varieties for kidney patients and diabetic patients, which comprises the following specific steps:

[0030] Step one: selecting parent materials: a mutant variety LGC-1 with low water-soluble protein introduced from the Radioactive Breeding Field of the Biological Resources Institute of Japan is used as the female parent, and a Qingyou 308 rice variety selected by the Rice Institute of Fujian Academy of Agricultural Sciences is used as the male parent, and then hybridization is carried out;

[0031] Step two: after obtaining hybrid offspring by hybridization, selfing is carried out, and the seeds of the offspring are analyzed from F1 to screen out seeds with low glutelin traits and high prolamin traits; wherein the glutelin content of the seeds with low glutelin traits is 3%; the prolamin content of the seeds with high prolamin traits is 60%; in addition, in this step, the seeds are analyzed by sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis;

[0032] Step three: selecting seeds with low glycemic index in the seeds with low glutelin traits and high prolamin traits; wherein the glycemic index of the seeds with low glycemic index is 45; in addition, in this step, the glycemic index in the seeds is analyzed by an instrument, and the instrument includes a high-performance liquid chromatograph;

[0033] Step four: selecting seeds with high amylose in seeds with low glycemic index; wherein the seeds with high amylose have an amylose content of 30%; in addition, in this step, the amylose in the seeds is analyzed by near-infrared analysis method;

[0034] The specific process of the near-infrared analysis method is as follows:

[0035] Process one: obtain the reference value of the amylose content of the sample single seed by using the "Determination of amylose content in rice, corn and millet grains" (GB 7648-87), establish a reference value matrix, and obtain the experimental standard deviation of the experimenter according to the national standard method;

[0036] Process two: pre-process the spectrum, select effective wavelengths, increase the signal-to-noise ratio, and eliminate interference;

[0037] Process three: extract the spectral characteristics, reduce the dimension of the data using PLS, and establish a spectral matrix;

[0038] Process four: use PLSR method and SVR method to correlate the information of the spectral matrix and the reference value matrix through chemometrics method, and establish a crop single seed amylose detection model;

[0039] Process five: after collecting the sample diffuse reflectance spectrum, remove abnormal spectra, and obtain the original spectrum of the sample, at this time, the amylose of the seed can be analyzed through the crop single seed amylose detection model;

[0040] Step five: thus, the rice seed with low glutelin trait, high prolamin trait, low glycemic index and high amylose is obtained.

[0041] Example 2:

[0042] The application provides a breeding method of rice varieties for kidney disease and diabetes patients, which comprises the following specific steps:

[0043] Step one, selecting parent materials: taking the low water-soluble protein mutant variety LGC-1 introduced from the Radioactive Breeding Field of the Japanese Biological Resources Institute as the female parent, and taking the Qingyou 308 rice variety selected by the Rice Institute of Fujian Provincial Academy of Agricultural Sciences as the male parent, and performing hybridization;

[0044] Step two: obtaining hybrid offspring by hybridization, and performing self-crossing, and analyzing the seeds of the offspring from F1 to screen out seeds with low glutelin trait and high prolamin trait; wherein the seeds with low glutelin trait have a glutelin content of 3%-4%; the seeds with high prolamin trait have an alcohol-soluble protein content of 61%; in addition, in this step, the seeds are analyzed by sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis;

[0045] Step three: selecting seeds with low glycemic index from seeds with low glutelin traits and high prolamin traits; wherein the seeds with low glycemic index have a glycemic index of 46; in addition, in this step, the glycemic index in the seeds is analyzed by an instrument, and the instrument includes a mass spectrometer;

[0046] Step four: selecting seeds with high amylose from seeds with low glycemic index; wherein the seeds with high amylose have an amylose content of 32.5%; in addition, in this step, the amylose in the seeds is analyzed by near-infrared analysis method;

[0047] The specific process of the near-infrared analysis method is as follows:

[0048] Process one: obtaining the amylose content reference value of the sample single seed by using 'Rice, Corn, Millet Grain Amylose Determination Method' (gb7648-87), establishing a reference value matrix, and obtaining the experimental standard deviation of the experimenter according to the national standard method;

[0049] Process two: pre-processing the spectrum, selecting effective wavelengths, increasing the signal-to-noise ratio, and eliminating interference;

[0050] Process three: extracting spectral features, reducing dimensions using PLS, and establishing a spectral matrix;

[0051] Process four: using PLSR method and SVR method to correlate and analyze the information of the spectral matrix and the information of the reference value matrix through chemometrics method, and establishing a crop single seed amylose detection model;

[0052] Process five: after collecting the sample diffuse reflectance spectrum, removing abnormal spectra, obtaining the original spectrum of the sample, at this time, the amylose of the seeds can be analyzed through the crop single seed amylose detection model;

[0053] Step five: thus, the rice seeds with low glutelin traits, high prolamin traits, low glycemic index and high amylose are obtained.

[0054] Example 3:

[0055] The present application provides a kind of kidney disease and the breeding method of special rice variety for diabetic, including specific steps as follows:

[0056] Step one, selecting parent material: with the low water-soluble protein mutant variety LGC-1 introduced from Japan Biological Resources Institute Radiation Breeding Field as female parent, with Qingyou 308 rice variety selected by Fujian Academy of Agricultural Sciences Rice Institute as male parent, hybridization is carried out;

[0057] Step two: obtain hybrid offspring by hybridization, and self-crossing, and analyze the seed of the offspring starting from F1 to screen out the seed with low glutelin trait and high prolamin trait; wherein the seed with low glutelin trait has a glutelin content of 3-4%; the seed with high prolamin trait has a prolamin content of 62.5%; in addition, in this step, the seed is analyzed by sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis;

[0058] Step three: select the seed with low glycemic index from the seed with low glutelin trait and high prolamin trait; wherein the seed with low glycemic index has a glycemic index of 47.5; in addition, in this step, the glycemic index in the seed is analyzed by an instrument, and the instrument includes an infrared spectrometer;

[0059] Step four: select the seed with high amylose from the seed with low glycemic index; wherein the seed with high amylose has an amylose content of 35%; in addition, in this step, the amylose in the seed is analyzed by near-infrared analysis method;

[0060] The specific process of the near-infrared analysis method is as follows:

[0061] Process one: obtain the amylose content reference value of the sample single grain by using "Rice, Corn, Millet Grain Amylose Determination Method" (gb7648-87), establish a reference value matrix, and obtain the experimental standard deviation of the experimenter according to the national standard method;

[0062] Process two: pretreat the spectrum, select effective wavelengths, increase the signal-to-noise ratio, and eliminate interference;

[0063] Process three: extract the spectral characteristics, reduce the dimension of the data by using PLS, and establish a spectral matrix;

[0064] Process four: use PLSR method and SVR method to regress and correlate the information of the spectral matrix and the information of the reference value matrix through chemometrics method, and establish a crop single grain seed amylose detection model;

[0065] Process five: after collecting the sample diffuse reflectance spectrum, eliminate abnormal spectrum, and obtain the original spectrum of the sample; at this time, the amylose of the seed can be analyzed through the crop single grain seed amylose detection model;

[0066] Step five: thus, the rice seed with low glutelin trait, high prolamin trait, low glycemic index and high amylose is obtained.

[0067] Example 4:

[0068] The application provides a breeding method of rice varieties special for kidney disease and diabetes patients, which comprises the following specific steps:

[0069] Step one, selecting parent material: a mutant variety LGC-1 with low water-soluble protein introduced from the Radiation Breeding Field of the Biological Resources Institute of Japan is taken as the female parent, and Qingyou 308 rice variety selected by the Rice Institute of Fujian Academy of Agricultural Sciences is taken as the male parent to carry out hybridization;

[0070] Step two: hybrid offspring is obtained through hybridization, and self-crossing is carried out, and the offspring seeds are analyzed from F1 to screen out seeds with low glutelin and high prolamin; the glutelin content of the seeds with low glutelin is 3%-4%; the prolamin content of the seeds with high prolamin is 63%; in addition, the seeds are analyzed through sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis in this step;

[0071] Step three: seeds with low glycemic index are selected from the seeds with low glutelin and high prolamin; the glycemic index of the seeds with low glycemic index is 48.5; in addition, the glycemic index in the seeds is analyzed through instruments in this step, and the instruments include a mass spectrometer;

[0072] Step four: seeds with high amylose are selected from the seeds with low glycemic index; the amylose content of the seeds with high amylose is 37.5%; in addition, the amylose in the seeds is analyzed through near-infrared analysis method in this step;

[0073] The specific process of the near-infrared analysis method is as follows:

[0074] Process one: the amylose content reference value of the sample single grain is obtained by using "Determination of Amylose Content in Rice, Corn and Millet Grains" (GB 7648-87), a reference value matrix is established, and the experimental standard deviation of the experimenter is obtained according to the national standard method;

[0075] Process two: the spectrum is pretreated, the effective wavelength is selected, the signal-to-noise ratio is increased, and the interference is eliminated;

[0076] Process three: the spectrum characteristics are extracted, PLS is used to reduce the dimension of the data, and a spectrum matrix is established;

[0077] Process four: the information of the spectrum matrix and the information of the reference value matrix are analyzed through regression correlation analysis by using PLSR method and SVR method, and a crop single-seed seed amylose detection model is established;

[0078] Process five: after collecting the sample diffuse reflectance spectrum, the abnormal spectrum is removed, and the original spectrum of the sample is obtained, at this time, the amylose of the seeds can be analyzed through the crop single-seed seed amylose detection model;

[0079] Step five: to obtain the rice seeds with low glutelin trait, high prolamin trait, low glycemic index and high amylose.

[0080] Example 5:

[0081] The present application provides a method for breeding rice varieties for kidney disease and diabetes patients, comprising the following specific steps:

[0082] Step one, selecting parent materials: using the low water-soluble protein mutant variety LGC-1 introduced from the Radiation Breeding Field of the Japanese Biological Resources Institute as the female parent, and the Qingyou 308 rice variety selected by the Rice Institute of Fujian Provincial Academy of Agricultural Sciences as the male parent, hybridization was carried out;

[0083] Step two: obtain hybrid offspring by hybridization and self-crossing, and analyze the seeds of the offspring from F1 to screen out seeds with low glutelin trait and high prolamin trait; wherein the glutelin content of the seeds with low glutelin trait is 3%-4%; the prolamin content of the seeds with high prolamin trait is 65%; in addition, in this step, the seeds are analyzed by sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis;

[0084] Step three: select seeds with low glycemic index from the seeds with low glutelin trait and high prolamin trait; wherein the glycemic index of the seeds with low glycemic index is 50; in addition, in this step, the glycemic index in the seeds is analyzed by an instrument, which includes a high performance liquid chromatograph;

[0085] Step four: select seeds with high amylose from the seeds with low glycemic index; wherein the amylose content of the seeds with high amylose is 40%; in addition, in this step, the amylose in the seeds is analyzed by near-infrared analysis method;

[0086] The specific process of near-infrared analysis method is as follows:

[0087] Process one: obtain the reference value of amylose content of single grain of the sample by "Determination of amylose content in rice, maize and millet grains" (gb7648-87), establish the reference value matrix, and obtain the experimental standard deviation of the experimenter according to the national standard method;

[0088] Process two: pretreat the spectrum, select effective wavelength, increase signal-to-noise ratio, and eliminate interference;

[0089] Process three: extract the spectral characteristics, reduce the dimension of the data by PLS, and establish the spectral matrix;

[0090] Process four: use PLSR method and SVR method to correlate the information of the spectral matrix and the information of the reference value matrix by chemometrics method, and establish the detection model of amylose content in single grain of crop seeds;

[0091] Process five: after collecting the sample diffuse reflectance spectrum, the abnormal spectrum is removed to obtain the original spectrum of the sample, at this time, the amylose of the seed can be analyzed by the crop single seed amylose detection model;

[0092] Step five: thus, the rice seed with low glutelin trait, high prolamin trait, low glycemic index and high amylose is obtained.

[0093] The breeding methods used in the above examples 1-5 are compared, specifically, 50 volunteers with kidney disease are randomly selected and divided into 5 groups, 10 people in each group, and each group corresponds to a group of examples, after eating the corresponding rice, their evaluation is as follows:

[0094] Full marks 10 0-5 6-8 9-10 Example 1 1 2 7 Example 2 2 1 7 Example 3 0 1 9 Example 4 1 3 6 Example 5 1 1 8

[0095] From the above table, after use, example 3 is the best.

[0096] The breeding methods used in the above examples 1-5 are compared, specifically, 50 volunteers with kidney disease are randomly selected and divided into 5 groups, 10 people in each group, and each group corresponds to a group of examples, after eating the corresponding rice, their evaluation is as follows:

[0097] Full marks 10 0-5 6-8 9-10 Example 1 2 2 6 Example 2 1 2 7 Example 3 0 1 9 Example 4 1 1 8 Example 5 1 2 7

[0098] From the above table, after use, example 3 is the best.

[0099] Although the present application has been described above with reference to the implementation, various improvements can be made and equivalent substitutions can be made to the components thereof without departing from the scope of the present application. In particular, each feature disclosed in the embodiments of the present application can be combined with any other feature in any manner, provided that there is no structural conflict. The combinations of these features are not exhaustively described in the specification only for the purpose of saving space and resources. Therefore, the present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A method for breeding rice varieties for kidney disease and diabetes patients, characterized by, The specific steps are as follows: Step one, selecting parent materials: taking the mutant variety LGC-1 with low water-soluble protein introduced from the Radiation Breeding Field of the Biological Resources Institute of Japan as the female parent, and taking the Qingyou 308 rice variety bred by the Rice Institute of Fujian Academy of Agricultural Sciences as the male parent, to carry out hybridization; Step two: obtaining hybrid offspring by hybridization, and selfing, and analyzing the seeds of the offspring from F1 to screen out seeds with low glutelin traits and high prolamin traits; Step three: selecting seeds with low glycemic index from the seeds with low glutelin traits and high prolamin traits; Step four: selecting seeds with high amylose from the seeds with low glycemic index; Step five: thus, rice seeds with low glutelin traits, high prolamin traits, low glycemic index and high amylose are obtained.

2. The method of claim 1, wherein the rice variety is selected from the group consisting of NERICA, Oryza sativa L. indica, Oryza sativa L. japonica, and Oryza sativa L. indica-japonica. The glutelin content of the seeds with low glutelin traits in step two is 3%-4%.

3. The method for breeding a rice variety specifically for patients with kidney disease and diabetes according to claim 1, characterized in that, The prolamin content of the seeds with high prolamin traits in step two is 60%-65%.

4. The method for breeding a rice variety specifically for patients with kidney disease and diabetes according to claim 1, characterized in that, The glycemic index of the seeds with low glycemic index in step three is 45-50.

5. The method of claim 1, wherein the rice variety is selected from the group consisting of NERICA, Oryza sativa L. indica, Oryza sativa L. japonica, and Oryza sativa L. indica-japonica. The amylose content of the seeds with high amylose in step four is 30%-40%.

6. The method for breeding a rice variety specifically for patients with kidney disease and diabetes according to claim 1, characterized in that, In step two, the seeds are analyzed by sodium dodecyl sulfate denaturation-polyacrylamide gel electrophoresis.

7. The method for breeding a rice variety specifically for patients with kidney disease and diabetes according to claim 1, characterized in that, In step three, the glycemic index of the seeds is analyzed by instruments.

8. The method of claim 7, wherein the rice variety is selected from the group consisting of NERICA, Oryza sativa L. indica, Oryza sativa L. japonica, and Oryza sativa L. indica-japonica. The instruments include high performance liquid chromatograph, mass spectrometer and infrared spectrometer.

9. The method of claim 1, wherein the rice variety is selected from the group consisting of NERICA, Oryza sativa L. indica, Oryza sativa L. japonica, and Oryza sativa L. indica-japonica. In step three, the amylose in the seeds is analyzed by near-infrared analysis method.

10. The method of claim 9, wherein the rice variety is selected from the group consisting of NERICA, Oryza sativa, Oryza glaberrima, and mixtures thereof. The specific process of the near-infrared analysis method is as follows: Process one: the content reference value of amylose in single rice grain of the sample is obtained by the method for determination of amylose in rice, corn and millet grains (gb7648-87), a reference value matrix is established, and the experimental standard deviation of the experimenter is obtained according to the national standard method; Process two: the spectrum is pretreated, the effective wavelength is selected, the signal-to-noise ratio is increased, and the interference is eliminated; Process three: the spectral characteristics are extracted, the data are reduced by PLS, and a spectral matrix is established; Process four: the information of the spectral matrix and the information of the reference value matrix are regressed and correlated by chemometrics method by using PLSR method and SVR method, and a detection model of single rice grain seed amylose of crops is established; Process five: after collecting the diffuse reflectance spectrum of the sample, the abnormal spectrum is removed, and the original spectrum of the sample is obtained, at this time, the amylose of the seeds can be analyzed by the detection model of single rice grain seed amylose of crops.