Device for fixing eyeball extraction mark and positioning choroid paving sheet of experimental animal

Abstract

The invention provides an experimental animal eyeball extraction mark fixing and choroid paving piece positioning device which comprises an annular adjustable ferrule assembly and a square positioning ferrule assembly, the annular adjustable ferrule assembly and the square positioning ferrule assembly are of detachable structures, and the annular adjustable ferrule assembly is connected to the upper surface of the square positioning ferrule assembly in a clamped mode; the annular adjustable ferrule assembly is an annular main body and comprises an adjustable inner ring, the inner diameter of the adjustable inner ring can be elastically adjusted to adapt to eyeballs of different sizes, the technical field of ophthalmology basic experimental animal research equipment is involved, and the inner diameter of the adjustable inner ring of the annular adjustable ferrule assembly can be elastically adjusted to adapt to experimental rodent eyeballs of different sizes. The eyeball marking and taking-out device has the advantages that small eyeballs of young rats and large eyeballs of adult rats can be tightly attached to each other, accordingly, the stability of the eyeballs in marking and taking-out procedures can be guaranteed, eyeball injuries due to improper matching of the ferrules and the eyeballs can be prevented, the application range of the eyeball marking and taking-out device can be expanded, and the universality and the practicability of the eyeball marking and taking-out device can be improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of experimental animal research equipment, in particular to a device for marking and fixing eyeballs of experimental animals and positioning choroids. BACKGROUND

[0002] In the field of ophthalmic experimental rodents (rats / mice) eye ball related research, it is of great significance to analyze the retina choroid of the nasal and temporal sides of the eyeball in depth. For example, in the process of ophthalmic disease drug research and development, it is necessary to understand the difference in the intervention effect of drugs on the retina in different parts of the eyeball; in the study of the physiological structure of the retina tissue, it is necessary to accurately measure the thickness of the nasal and temporal retina choroid and other parameters. The premise of realizing these accurate analyses is to accurately mark the eye position direction, and to develop the retina choroid along the pupil optic nerve axis during the process of retina choroid spreading, combined with the above-mentioned nasal and temporal retina choroid marking, to accurately position the four quadrants of the retina choroid (i.e. nasal superior, nasal inferior, temporal superior, and temporal inferior).

[0003] However, the current method has serious defects. First, in the eye marking process, due to the lack of specially designed marking tools, researchers often have to rely on simple staining methods or relatively rough operations to try to distinguish the nasal and temporal sides of the eyeball. However, the marks formed by these methods are not clear, and it is difficult to clearly and accurately distinguish the nasal and temporal sides before or after the eyeball is removed, which brings a large error to the subsequent retina positioning. Once the eye position direction marking is not accurate, the analysis of the tissue related to different directions of the retina based on this may lead to incorrect conclusions, affecting the reliability of the entire experiment.

[0004] Secondly, in terms of retina choroid spreading positioning, the traditional fixation and direction marking method has obvious shortcomings. Due to the lack of effective positioning means, it is difficult to ensure that the eyeball is in the ideal axial position (i.e. pupil optic nerve axis) when the retina choroid is spread. When the retina choroid of the eyeball is fixed, the direction error or deviation will result in the inability to obtain the longitudinal (i.e. pupil optic nerve axis direction) spread of the four quadrants of the retina choroid, leading to the inability to distinguish the quadrant position of the retina choroid after spreading. When the above spreading direction is wrong, it is very likely to cause the inconsistency of the retina choroid quadrants. Once this happens, it is difficult to distinguish the quadrant direction of the eyeball, and researchers often need to repeat the experiment, which undoubtedly greatly increases the repetitive cost of the experiment, including time cost, experimental animal cost, and the use cost of related reagents and equipment, etc.

[0005] In summary, existing methods for handling rodent eyeballs severely limit the accuracy and efficiency of research. Developing a device that integrates eyeball removal marking, orientation calibration, and precise retinal and choroidal flap placement is urgently needed. This is crucial for improving the reliability of experimental results and advancing ophthalmological research. Summary of the Invention

[0006] To overcome existing problems, this application provides a device for marking, fixing, and positioning the eyeballs of experimental animals and the choroidal flap. Through the unique marking button design on the ring-shaped adjustable ring assembly, the eye position of experimental rodents is accurately marked. The nasal and temporal sides can be clearly distinguished before the eyeball is removed, which greatly reduces the subsequent retinal positioning error. The integrated operation process organically combines the steps of eyeball marking, removal, fixing, and retinal-choroidal flap positioning, making the operation smooth and convenient, and making the experimental results more accurate and reliable.

[0007] The technical solution adopted by the embodiments of this application to solve its technical problem is: A device for marking and fixing the eyeball of an experimental animal and positioning the choroidal flap includes an adjustable ring assembly and a square positioning ring assembly. The adjustable ring assembly and the square positioning ring assembly are detachable structures, and the adjustable ring assembly is snapped onto the upper surface of the square positioning ring assembly. The adjustable ring assembly is a circular body with an adjustable inner ring. The inner diameter of the adjustable inner ring can be flexibly adjusted to fit eyeballs of different sizes.

[0008] Preferably, the square positioning collar assembly includes a frame and a puncture needle. The puncture needle is made of medical-grade stainless steel, with a sharp tip and good toughness. The tip is specially polished to easily penetrate the cornea and is not easily bent or broken during penetration. If fixation fluid is required, it can ensure that the fixation fluid can smoothly penetrate into the eyeball. The tip of the puncture needle is aligned with the center of the cornea, and pressing it can penetrate the cornea, ensuring that the fixation fluid fully penetrates into the eyeball. The frame is designed to be vertical, forcing the eyeball to maintain the vertical direction of the pupil-optic nerve axis. The frame is square in shape with rounded corners. The frame also has a groove inside, the size of which is the same as the size of the annular adjustable collar assembly. The puncture needle is detachably connected to the frame, and the needle body is engraved with scale markings. The square positioning collar assembly can be nested inside the annular adjustable collar assembly.

[0009] Preferably, the annular adjustable sleeve assembly further comprises an outer ring, which is made of high-strength and transparent medical-grade plastic material, such as polycarbonate, which has good mechanical properties and can withstand external forces that may be applied during operation to ensure the stability of the device, and its transparent property facilitates observation of the state of the eyeball and the operation of the marker button, and the outer ring is further provided with a handle on one side for holding, which is ergonomically designed with a certain arc and friction on the surface to facilitate the operator to hold and operate, and the outer ring is provided with a marker button at the position corresponding to the maximum width of the limbus, and the marker button is designed in a pressing manner and is internally provided with a microcavity for storing methylene blue dye, and the microcavity is connected to a liquid outlet hole on the surface of the marker button through a small pipeline.

[0010] Preferably, after the annular adjustable sleeve assembly is placed around the eyeball, it can be slid down to the optic nerve, and the marker button is used to pre-mark the direction of the eye position, and the eyeball can be taken out completely.

[0011] Preferably, the adjustable inner ring is connected to the outer ring through uniformly distributed elastic connecting structures, and the adjustable inner ring is pressed to extrude the elastic connecting structures, and the adjustable inner ring is made of silicone material with good elasticity and biocompatibility, the elasticity of the silicone material can realize flexible adjustment of the inner diameter to adapt to eyeballs of different sizes, and the silicone material has no stimulation to the eyeball tissue and will not affect the experimental results, and the surface of the silicone material is treated in a special way and has a certain friction to better fix the eyeball and prevent it from sliding in the sleeve.

[0012] The advantages of the embodiment of the application are: The adjustable inner ring of the annular adjustable sleeve assembly can elastically adjust the inner diameter to adapt to eyeballs of different sizes of experimental rodents, whether the eyeballs of young mice are small or the eyeballs of adult mice are relatively large, the adjustable inner ring can tightly fit the eyeballs to ensure the stability of the eyeballs during marking and taking out, avoid eyeball damage caused by improper fitting of the sleeve and the eyeball, expand the application range of the device and improve the universality and practicality of the device.

[0013] Through the unique design of the marker button on the annular adjustable sleeve assembly, precise marking of the eye position direction of the eyeball of the experimental rodent is realized, and the nasal side and the temporal side can be clearly distinguished before the eyeball is taken out, which greatly reduces the subsequent retinal positioning error, and the integrated operation process organically combines the steps of eyeball marking, taking out, fixing and retinal choroid plexus sheet positioning, which is convenient and continuous, and makes the experimental results more accurate and reliable.

[0014] The frame of the device is vertically erected, which can force the eyeball to keep the vertical direction of the pupil optic nerve axis, that is, to assist the retinal choroid to be unfolded along the pupil optic nerve axis during the retinal choroid unfolding process, combined with the above nasal and temporal retinal choroid markers, the four quadrants (i.e. nasal upper, nasal lower, temporal upper and temporal lower) of the retinal choroid can be accurately positioned, the unfolding is ensured to be carried out in the correct direction, the accuracy of the retinal choroid unfolding is greatly improved, the experimental failure caused by positioning deviation is reduced, the experimental efficiency is improved, and the experimental cost is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0015] The application will be further described below in combination with the drawings and examples.

[0016] Fig. 1 Fig. 1 is a top view of the device for marking and fixing the eyeball of an experimental animal and positioning the choroid; Fig. 2 Fig. 2 is a top view of the square positioning ring assembly of the device for marking and fixing the eyeball of an experimental animal and positioning the choroid; Fig. 3 Fig. 3 is a top view of the annular adjustable ring assembly of the device for marking and fixing the eyeball of an experimental animal and positioning the choroid.

[0017] Main figure mark explanation: 1, square positioning ring assembly; 11, frame; 12, groove; 13, puncture needle; 2, annular adjustable ring assembly; 21, outer ring; 22, adjustable inner ring; 23, handle; 24, marking button. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the application will be clearly and completely described below in combination with the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, not all the embodiments. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the application. In addition, in order to facilitate the description, the "up", "down", "left", "right" and the like in the drawings are consistent with the up, down, left and right of the drawings, and the "first", "second" and the like in the following text are for description and differentiation, and have no other special meanings.

[0019] The embodiment of the application provides an experimental animal eyeball taking-out mark fixing and choroid spreading positioning device, solves the problems in the prior art, the frame of the device is vertically arranged, the eyeball can be forced to keep the vertical direction of the pupil optic nerve axis, that is, the spreading and spreading in the process of retinal choroid spreading are performed along the pupil optic nerve axis, the four quadrants (i.e., nasal upper, nasal lower, temporal upper and temporal lower) of the retinal choroid can be accurately positioned in combination with the above-mentioned nasal side and temporal side retinal choroid marks, the spreading and spreading are ensured to be performed in the correct direction, the accuracy of the retinal choroid spreading is greatly improved, experimental errors caused by positioning deviation are reduced, experimental efficiency is improved, and experimental cost is reduced; the adjustable inner ring of the annular adjustable sleeve assembly can be elastically adjusted in diameter to adapt to eyeballs of different sizes of experimental rodents. Whether it is the smaller eyeball of a young mouse or the relatively larger eyeball of an adult mouse, the eyeball can be closely fitted to ensure the stability of the eyeball during the marking and taking-out process, avoid eyeball damage caused by improper fitting of the sleeve and the eyeball, expand the application range of the device, and improve the universality and practicality of the device. Through the unique mark button design on the annular adjustable sleeve assembly, accurate marking of the eye position direction of the experimental rodent eyeball is realized, the nasal side and the temporal side can be clearly distinguished before the eyeball is taken out, the subsequent retinal positioning error is greatly reduced, the integrated operation process organically combines the steps of eyeball marking, taking-out, fixing and retinal choroid spreading positioning, and is convenient and continuous, so that the experimental results are more accurate and reliable.

[0020] The technical scheme in the embodiment of the application is used for solving the above problems, and the general idea is as follows: Embodiment The embodiment provides a specific structure of a rodent eyeball taking-out / positioning marking, paraffin puncture fixing and retinal choroid spreading positioning fixing device, as shown in the drawing, which comprises an annular adjustable sleeve assembly 2 and a square positioning sleeve assembly 1. Figs. 1-3 The annular adjustable sleeve assembly 2 and the square positioning sleeve assembly 1 are detachable structures, and the annular adjustable sleeve assembly 2 is clamped on the upper surface of the square positioning sleeve assembly 1. The annular adjustable sleeve assembly 2 is a circular ring body, comprising an adjustable inner ring 22, the inner diameter of the adjustable inner ring 22 can be elastically adjusted to adapt to eyeballs of different sizes, a handle 23 of the annular adjustable sleeve assembly 2 is held, the annular adjustable sleeve assembly 2 is close to the eyeball of the experimental rodent, according to the size of the eyeball, the adjustable inner ring 22 is slightly stretched or extruded, the inner diameter of the adjustable inner ring 22 is adapted to the size of the eyeball, the annular adjustable sleeve assembly 2 is continuously slid down to the optic nerve, and then the annular adjustable sleeve assembly 2 is slightly pulled out, so that the eyeball is completely taken out from the head of the animal.

[0021] The square positioning collar assembly 1 comprises a frame 11 and a puncture needle 13, the puncture needle 13 is made of medical-grade stainless steel, has a sharp needle tip and good toughness, the needle tip is specially polished, can easily penetrate the cornea, and is not easy to bend or break during penetration, if it needs to be fixed, it can ensure that the fixing liquid can smoothly penetrate into the eyeball, the puncture needle 13 is aimed at the center of the cornea, and can penetrate the cornea after pressing, ensuring that the fixing liquid fully penetrates into the eyeball, the frame 11 is designed to be vertically erected, forcing the eyeball to maintain the vertical direction of the pupil optic nerve axis, the frame 11 is square as a whole, the four corners are treated with round corners, and the frame 11 is further provided with a groove 12 inside, the size of the groove 12 is the same as that of the annular adjustable collar assembly 2, the puncture needle 13 is detachably connected with the frame 11, and a scale mark is engraved on the needle body. The square positioning collar assembly 1 can be nested in the annular adjustable collar assembly 2, the frame 11 is made of corrosion-resistant and high-strength metal material, such as stainless steel, which has good chemical corrosion resistance and will not corrode or deform under the action of chemical reagents such as fixing liquid, dehydrating agent and paraffin, thereby ensuring the service life of the device, and meanwhile, the high-strength characteristic can provide stable support for the eyeball during paraffin tissue sectioning.

[0022] The annular adjustable collar assembly 2 further comprises an outer ring 21, the outer ring 21 is made of high-strength and transparent medical-grade plastic material, such as polycarbonate, which has good mechanical properties and can withstand external forces that may be applied during operation, thereby ensuring the stability of the device, and meanwhile, the transparent characteristic facilitates observation of the state of the eyeball and the operation of the marking button, the outer ring 21 is further provided with a handle 23 on one side for holding, the handle 23 is designed in accordance with human engineering, has a certain arc and friction on the surface, and is convenient for the operator to hold and operate, and the outer ring 21 is provided with a marking button 24 at the position corresponding to the maximum width of the limbus, the marking button 24 is designed in a pressing manner and is internally provided with a micro cavity for storing methylene blue dye, and the micro cavity is connected with a liquid outlet hole on the surface of the marking button 24 through a small pipeline; In a further embodiment, the marking button 24 is located at the position corresponding to the maximum width of the limbus of the outer ring 21 and is designed in a pressing manner, the marking button 24 is internally provided with a micro cavity for storing methylene blue dye, and the cavity is connected with a liquid outlet hole on the surface of the button through a small pipeline, when the marking button 24 is pressed, the internal pressure changes to make the methylene blue dye extrude through the liquid outlet hole to form a positioning color mark on the surface of the sclera, and meanwhile, the surface of the marking button 24 is designed with anti-slip texture to facilitate the operator to press accurately, and meanwhile, the marking button 24 is marked with obvious marks to indicate its function and pressing direction, which can ensure that the retinal choroid is developed and spread along the pupil optic nerve axis during the retinal choroid sectioning process, and in combination with the above nasal and temporal retinal choroid markers, the four quadrants (i.e. nasal upper, nasal lower, temporal upper and temporal lower) of the retinal choroid can be accurately positioned, thereby ensuring that the retinal choroid is developed and spread in the correct direction.

[0023] The annular adjustable sleeve assembly 2 is sleeved on the eyeball and can slide down to the optic nerve, and the eye position direction is pre-marked by the marking button 24, and the eyeball is taken out completely.

[0024] The adjustable inner ring 22 is connected with the outer ring 21 through the uniformly distributed elastic connection structure, the adjustable inner ring 22 is pressed and extruded by the elastic connection structure, the adjustable inner ring 22 is made of silica gel material with good elasticity and biocompatibility, the elasticity of the silica gel can realize flexible adjustment of the inner diameter to adapt to eyeballs of different sizes, and the silica gel has no stimulation to the eyeball tissue and will not affect the experimental results, the surface of the silica gel is treated specially and has a certain friction, so that the eyeball can be better fixed and prevented from sliding in the sleeve.

[0025] By adopting the above technical scheme: The operator holds the handle 23 of the annular adjustable sleeve assembly 2, and places it close to the eyeball of the experimental rodent, according to the size of the eyeball, the adjustable inner ring 22 is gently stretched or extruded, so that the inner diameter is adapted to the size of the eyeball, and the adjustable inner ring 22 is tightly combined with the surface of the eyeball, but will not cause excessive compression to the eyeball.

[0026] The annular adjustable sleeve assembly 2 is slowly slid down to the maximum diameter of the limbus, at this time, the operator presses the marking button 24 with the thumb, so that the methylene blue dye forms a clear positioning color mark on the surface of the sclera, and the nasal side or temporal side direction of the eyeball is marked.

[0027] After the marking is completed, the annular adjustable sleeve assembly 2 is continuously slid down to the optic nerve, and then is gently pulled out, so that the eyeball is completely taken out from the head of the animal.

[0028] The taken-out eyeball with the mark is slowly sleeved into the frame 11 of the square positioning sleeve assembly 1 together with the annular adjustable sleeve assembly 2. It is ensured that the frame 11 is tightly nested with the annular adjustable sleeve assembly 2, and the needle tip of the puncture needle 13 is accurately aligned with the center of the cornea.

[0029] The puncture needle 13 is gently pressed by the finger, so that it penetrates the cornea, after the puncture is completed, the whole device is immersed into the container containing the fixing liquid, and the fixing time is two hours. During the fixing process, the fixing liquid fully penetrates into the eyeball through the channel formed by the puncture needle 13, so that the eyeball tissue maintains a stable form and structure.

[0030] After the fixing is completed, the device is sequentially subjected to dehydration and wax immersion treatment according to the conventional experimental steps. Different concentrations of ethanol solution are used in the dehydration process, so that the water in the eyeball tissue is gradually removed; the wax immersion uses paraffin with a suitable melting point, so that the paraffin fully penetrates into the eyeball tissue, and prepares for the subsequent embedding.

[0031] The ring device treated by dehydration and wax immersion is vertically erected in the embedding iron box, and the melted paraffin is slowly poured in. After the paraffin solidifies, the annular adjustable ring assembly 2 and the square positioning ring assembly 1 are carefully removed. At this time, the eyeball in the paraffin forms a standard unfolded and laid flat orientation along the optic nerve axis, that is, the unfolded and laid flat during the retinal choroid lay flat process is along the pupil optic nerve axis. Combined with the above nasal and temporal retinal choroid markers, the four quadrants (i.e. nasal upper, nasal lower, temporal upper, and temporal lower) of the retinal choroid can be accurately positioned, which can be used for subsequent unfolded and laid flat and experimental analysis.

[0032] Finally, it should be noted that: obviously, the above embodiments are only examples for clearly illustrating the present application, and are not limitations on the embodiments. Based on the above description, other different forms of changes or variations can also be made by those of ordinary skill in the art. Here, it is not necessary and impossible to exhaust all the embodiments. The obvious changes or variations derived therefrom are still within the protection scope of the present application.

Claims

1. An apparatus for marking and fixing the enucleation of the eyeball of an experimental animal and positioning the choroid spread, characterized by, Including annular adjustable ring assembly (2) and square positioning ring assembly (1), the annular adjustable ring assembly (2) and square positioning ring assembly (1) are detachable structure, the annular adjustable ring assembly (2) is clamped on the upper surface of the square positioning ring assembly (1); Wherein, the annular adjustable ring assembly (2) is a circular ring body, comprising an adjustable inner ring (22), the inner diameter of the adjustable inner ring (22) can be elastically adjusted to adapt to different size eyeball.

2. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of an experimental animal according to claim 1, characterized in that, The square positioning ring assembly (1) includes a frame (11) and a puncture needle (13), the puncture needle (13) is detachably connected with the frame (11), the needle body is marked with a scale mark, the square positioning ring assembly (1) can be nested in the annular adjustable ring assembly (2).

3. The device for marking and fixing the enucleated eyeball and positioning the choroid in the experimental animal according to claim 1, characterized in that, The annular adjustable ring assembly (2) further comprises an outer ring (21), the outer ring (21) is provided with a mark button (24) corresponding to the maximum width of the angular sclera.

4. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of an experimental animal according to claim 3, characterized in that, The annular adjustable ring assembly (2) can slide down to the optic nerve after covering the eyeball, and the mark button (24) is used for pre-marking the eye position direction, while assisting the complete removal of the eyeball.

5. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of an experimental animal according to claim 4, characterized in that, The outer ring (21) is further provided with a handle (23) on one side, which is used for holding.

6. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of an experimental animal according to claim 2, characterized in that, The puncture needle (13) is aimed at the center of cornea, and can penetrate the cornea after pressing, which can ensure that the fixing liquid fully penetrates into the eyeball.

7. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of an experimental animal according to claim 6, characterized in that, The frame (11) is designed to be vertically erected, which forces the eyeball to keep the vertical direction of the pupil optic nerve axis.

8. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of claim 5, wherein, The adjustable inner ring (22) is connected with the outer ring (21) through evenly distributed elastic connecting structure, and the adjustable inner ring (22) is pressed and extruded by the elastic connecting structure.

9. The device for marking and fixing the enucleated eyeball and positioning the choroid in the experimental animal according to claim 4, characterized in that, The mark button (24) is designed to be pressed, and a microcavity for storing methylene blue dye is arranged inside, and the microcavity is connected with the liquid outlet hole on the surface of the mark button (24) through a small pipeline.

10. The device for marking and fixing the enucleated eyeball and positioning the choroid spread of claim 5, wherein, The frame (11) is square as a whole, and the four corners are rounded, and the frame (11) is further provided with a recess (12) inside, and the size of the recess (12) is the same as that of the annular adjustable ring assembly (2).

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