A composition containing a fermented extract of stone fruit and a method of preparing the same

Abstract

The application discloses a kind of compositions containing stone peach fermented extract and preparation method thereof, and specifically belongs to the technical field of cosmetic compounding materials;The composition is composed of the following mass fraction components: 5-11 parts of stone peach fermented extract, 3-5 parts of rhizome of atractylodes lancea extract, 0.5-0.8 parts of centella asiatica extract and 55-69 parts of rose water;The stone peach fermented extract is extracted after fermentation by Eurotium cristatum, and the strain decomposes the fermentation substrate during fermentation, promotes the release and enrichment of active substances, and improves the efficacy of stone peach;The composition provided by the application has excellent antioxidant and oil control effects.

Description

Technical Field

[0001] This invention belongs to the technical field of cosmetic compound raw materials, specifically, it relates to a composition containing fermented extract of Prunus persica and its preparation method. Background Technology

[0002] Oily skin, due to excessive sebum secretion from the sebaceous glands, is prone to skin problems such as acne and seborrheic dermatitis, seriously affecting appearance and health. Currently, mainstream oil-control cosmetic solutions have the following limitations:

[0003] I. Chemical synthesis inhibitors have significant side effects.

[0004] While chemical 5α-reductase inhibitors are effective, they have systemic side effects such as sexual dysfunction and teratogenic risks, and are therefore not suitable for topical cosmetic use.

[0005] II. Bottlenecks in the Application of Dendrobium nobile

[0006] Although traditional extracts of Pholidota chinensis have antibacterial activity, their fat-soluble active ingredients have low dissolution rates and insufficient bioavailability. Furthermore, high-temperature extraction can easily destroy heat-sensitive substances. In addition, existing research has not adequately explored the development of Pholidota chinensis in the cosmetics field.

[0007] III. Fermentation technology is not fully utilized

[0008] Existing fermented cosmetics mostly focus on lactic acid bacteria / yeast fermentation (such as kombucha), while research on the application of medicinal fungi (such as Eurotium cristatum) in the transformation of plant active ingredients is lacking, resulting in insufficient enhancement of efficacy of active ingredients.

[0009] Therefore, developing a composition that enhances the efficacy of active ingredients in *Dendrobium nobile* through safe and efficient microbial fermentation technology and combines synergistic components to achieve multi-pathway oil control has become an urgent need in the industry. Summary of the Invention

[0010] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a composition that enhances the efficacy of active ingredients in *Prunus persica* through safe and efficient microbial fermentation technology and achieves multi-pathway oil control by compounding synergistic components.

[0011] To achieve the above objectives, the present invention discloses the following technical solutions:

[0012] In a first aspect, the present invention provides a composition containing a fermented extract of *Prunus persica*, wherein the composition comprises, by weight parts, the following components:

[0013] 5-11 parts of fermented extract of Prunus persica;

[0014] 3-5 parts of Atractylodes lancea rhizome extract;

[0015] Centella asiatica extract 0.5-0.8 parts;

[0016] 55-69 parts rose water.

[0017] Preferably, the preparation method of the fermented extract of *Prunus persica* includes the following steps:

[0018] Step 1-1. First, activate and isolate *Aspergillus cristatus* to obtain a suspension of *Aspergillus cristatus* spores;

[0019] Steps 1-2. Dry, pulverize, and sieve the pseudobulbs of *Dendrobium nobile* to obtain *Dendrobium nobile* powder;

[0020] Steps 1-3. Mix the powdered *Dendrobium nobile*, glucose, dipotassium hydrogen phosphate, MgSO4·7H2O, vitamin B1 and sterile water to obtain a solid fermentation medium containing *Dendrobium nobile*. Sterilize the fermentation medium at 115℃ for 20 minutes before use.

[0021] Steps 1-4. Take the *Aspergillus cristatus* spore suspension obtained in Step 1-1, and inoculate the bacterial suspension into a solid fermentation medium at 10-12% of the fermentation medium mass. Incubate at 28-30℃, aeration rate of 2-5 L / min·kg, and humidity of 70-80% RH for 5-7 days to obtain the fermentation material.

[0022] Steps 1-5. Mix and soak the fermentation material with an ethanol aqueous solution of 60-70 v / v% at a mass-volume ratio of 1:20-25 g / mL. Treat the mixture with an ultrasonic frequency of 20-30 kHz and a temperature of 50-60℃ for 30-40 min. Centrifuge to separate the supernatant. Concentrate the supernatant under reduced pressure at 55-60℃ to 1 / 3 of the original volume to obtain the fermented extract of *Prunus persica*.

[0023] More preferably, the concentration of the *Eurotium cristatum* spore suspension in step 1-1 is 1–5 × 10⁻⁶. 9 cfu / mL.

[0024] More preferably, in steps 1-2, the pseudobulbs of *Dendrobium nobile* are sliced, placed in a constant temperature drying oven and dried at 60°C to constant weight, and then pulverized in a high-speed pulverizer through a 60-mesh sieve to obtain *Dendrobium nobile* powder.

[0025] More preferably, in steps 1-3, the powdered *Dendrobium nobile*, glucose, dipotassium hydrogen phosphate, MgSO4·7H2O, vitamin B1, and sterile water are mixed in a mass ratio of 100:(5-7):(1-2):(0.5-1):(0.01-0.02):(90-100).

[0026] Preferably, the preparation method of the Atractylodes lancea rhizome extract includes the following steps:

[0027] Step 2-1. Take dried Atractylodes rhizome, crush and sieve it to obtain Atractylodes powder. Mix Atractylodes powder with petroleum ether at a mass-volume ratio of 1:(8-10) g / mL, and reflux extract twice at 70-80℃ for 1.5 h each time. After filtration, discard the filtrate and dry the resulting residue 1. Mix residue 1 with anhydrous ethanol at a mass-volume ratio of 1:(6-8) g / mL, and reflux extract three times at 70-80℃ for 1 h each time. After filtration, discard the filtrate and dry the resulting residue 2 to obtain defatted Atractylodes powder.

[0028] Step 2-2. Mix defatted Atractylodes lancea powder with deionized water at a mass-volume ratio of 1:(20-30)g / mL, soak at 80-90℃ and ultrasonic frequency of 28-30kHz for 30min, stop ultrasonication, keep at 80-90℃ for 3-4h for soaking and extraction, and centrifuge to obtain supernatant.

[0029] Steps 2-3. Concentrate the supernatant under reduced pressure at 60-70℃ to 1 / 6 of its original volume to obtain a concentrated solution. Add 95 v / v% ethanol aqueous solution to the concentrated solution to adjust the final ethanol concentration to 70-80 v / v%. Place the mixture in a refrigerator at 4℃ and let it stand for 10-12 hours. Then centrifuge to separate the precipitate. Place the precipitate in a vacuum drying oven and dry it at 50-60℃ for 2-3 hours to obtain the Atractylodes lancea rhizome extract.

[0030] More preferably, the dried Atractylodes rhizome described in step 2-1 is pulverized and passed through a 30-40 mesh sieve to obtain Atractylodes powder.

[0031] More preferably, in steps 2-3, the collected precipitate is washed with a small amount of 95 v / v ethanol aqueous solution, and then centrifuged, and this process is repeated twice.

[0032] Preferably, the method for preparing the rose water includes the following steps:

[0033] Take fresh rose petals and soak them in deionized water at a mass-to-volume ratio of 1:(25-30)g / mL for 2-3 hours. Then place them in an essential oil distillation extraction device and distill them for 2.5-3.5 hours using the material-water co-distillation method. Separate the rose essential oil and rose hydrosol using an oil-water separator. Select the rose hydrosol to obtain the rose water.

[0034] Secondly, the present invention provides the application of the composition described in the first aspect in the preparation of skin care products with oil-controlling and antioxidant effects.

[0035] In this invention:

[0036] The fermented extract of *Dendrobium nobile* obtained after fermentation by *Aspergillus cristatus* exhibits higher activity compared to the unfermented extract, demonstrating better efficacy in oil control and anti-oxidation. This is because *Aspergillus cristatus* secretes various active enzymes such as proteases and cellulases during the fermentation process, which efficiently degrade the plant cell wall, fully releasing active ingredients and avoiding the destruction of active ingredients by traditional extraction methods. At the same time, through biotransformation, the stable flavonoids in *Dendrobium nobile* are activated into small-molecule active aglycones, significantly enhancing its oil-controlling, antioxidant, and skin permeability, further improving the skin's utilization of active substances.

[0037] The Atractylodes lancea rhizome extract obtained through defatting and alcohol precipitation exhibits a strong scavenging ability against reactive oxygen species such as hydroxyl radicals and superoxide radicals in organisms, and also enhances the activity of antioxidant enzymes. It can protect biological membranes by inhibiting lipid peroxidation and also has a good anti-aging effect. This invention found that the combined use of Atractylodes lancea rhizome extract and fermented extract of Prunus persica can enhance their 5αR inhibition activity, thereby improving the oil-controlling ability of the formula.

[0038] The core component of Centella asiatica extract, asiaticoside, can inhibit the inflammatory pathway of sebaceous gland cells and reduce the release of sebum-promoting factors, thus blocking the "inflammation-oil production" cycle at its source. Simultaneously, it works synergistically with flavonoid aglycones from Dendrobium officinale to effectively scavenge free radicals, achieving the effects of anti-oxidative damage and inhibiting sebum synthesis.

[0039] The beneficial effects of this invention are:

[0040] 1. The 5α-reductase inhibition rate of the composition provided by the present invention is significantly higher than that of the blank control group. Among them, the 5α-reductase inhibition rate of the fermented extract of Prunus persica, the rhizome extract of Atractylodes lancea, the extract of Centella asiatica and the rose water in the composition at a mass ratio of 9:4:0.7:60 is the best, which is close to 60% of the performance of the positive control group. This indicates that the composition provided by the present invention has a strong inhibitory effect on 5α-reductase and can effectively inhibit the secretion of sebum in the skin, thereby achieving the effect of oil control.

[0041] 2. The compositions provided by the present invention have a DPPH scavenging rate of over 85% and exhibit excellent antioxidant capacity. Detailed Implementation

[0042] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0043] To further illustrate the present invention, the following embodiments are provided for detailed description. The raw materials used in the following embodiments and comparative examples of the present invention are all commercially available products.

[0044] In this invention:

[0045] Pholidota chinensis: A plant belonging to the genus Pholidota in the family Orchidaceae, commercially available;

[0046] Atractylodes rhizome: The rhizome of Atractylodes lancea (Thunb.) DC., a plant in the Asteraceae family, is commercially available;

[0047] Centella Asiatica extract: Centella Asiatica extract, purchased from Shandong Freda Biotechnology Co., Ltd.;

[0048] *Eurotium cristatum*: purchased from the China Industrial Microbial Culture Collection Center, strain number CICC: 41401.

[0049] Preparation of fermented extract of Prunus persica:

[0050] Step 1-1. First, activate and isolate *Aspergillus cristatus* to obtain a *Aspergillus cristatus* spore suspension with a concentration of 4 × 10⁻⁶. 9 cfu / mL;

[0051] Step 1-2. Slice the pseudobulb of *Dendrobium nobile*, place it in a constant temperature drying oven and dry it at 60°C until constant weight, then grind it in a high-speed pulverizer and pass it through a 60-mesh sieve to obtain *Dendrobium nobile* powder.

[0052] Steps 1-3. Mix the powdered *Dendrobium nobile*, glucose, dipotassium hydrogen phosphate, MgSO4·7H2O, vitamin B1, and sterile water to obtain a solid fermentation medium containing *Dendrobium nobile*. The mass ratio of *Dendrobium nobile* powder, glucose, dipotassium hydrogen phosphate, MgSO4·7H2O, vitamin B1, and sterile water is 100:6:1:0.5:0.01:100. Sterilize the fermentation medium at 115℃ for 20 minutes before use.

[0053] Steps 1-4. Take the *Aspergillus cristatus* spore suspension obtained in Step 1-1, and inoculate the bacterial suspension into a solid fermentation medium at 11% of the fermentation medium mass. Incubate at 28℃, aeration rate of 3L / min·kg, and humidity of 75%RH for 7 days to obtain the fermentation material.

[0054] Steps 1-5. Mix and soak the fermentation material with a 70 v / v% ethanol aqueous solution at a mass-volume ratio of 1:25 g / mL. Treat the mixture with an ultrasonic frequency of 28 kHz and a temperature of 60 ℃ for 30 min. Centrifuge to separate the supernatant. Concentrate the supernatant under reduced pressure at 60 ℃ to 1 / 3 of the original volume to obtain the fermented extract of *Prunus persica*.

[0055] Preparation of *Dendrobium nobile* extract:

[0056] The pseudobulb of *Dendrobium nobile* was sliced ​​and dried at 60°C to constant weight in a constant temperature drying oven. Then, it was pulverized in a high-speed pulverizer and passed through a 60-mesh sieve to obtain *Dendrobium nobile* powder. The *Dendrobium nobile* powder was mixed with a 75% v / v ethanol aqueous solution at a mass-volume ratio of 1:25 g / mL and treated with ultrasound at a frequency of 28 kHz and a temperature of 60°C for 30 min. After ultrasonic treatment, the mixture was transferred to a reflux extraction device and refluxed three times for 1 h each time. The filtrates were combined and concentrated under reduced pressure at 60°C to 1 / 3 of the original volume to obtain *Dendrobium nobile* extract.

[0057] Preparation of Atractylodes lancea rhizome extract:

[0058] Step 2-1. Place the dried Atractylodes lancea rhizome in a high-speed grinder and grind it through a 40-mesh sieve to obtain Atractylodes lancea powder. Mix the Atractylodes lancea powder with petroleum ether at a mass-volume ratio of 1:10 g / mL and reflux extract twice at 80℃ for 1.5 h each time. After filtration, discard the filtrate and dry the residue to remove the solvent. Mix the obtained residue with anhydrous ethanol at a mass-volume ratio of 1:8 g / mL and reflux extract three times at 80℃ for 1 h each time. After filtration, discard the filtrate and dry the residue to remove the solvent to obtain defatted Atractylodes lancea powder.

[0059] Step 2-2. Mix defatted Atractylodes lancea powder with deionized water at a mass-volume ratio of 1:30 g / mL, soak at 90℃ and ultrasonic frequency of 28-30 kHz for 30 min, stop ultrasonication, keep soaking and extracting at 90℃ for 3 h, and centrifuge to obtain supernatant.

[0060] Steps 2-3. Concentrate the supernatant under reduced pressure at 60℃ to 1 / 6 of its original volume to obtain a concentrate. Add 95 v / v% ethanol aqueous solution to the concentrate to adjust the final ethanol concentration to 80 v / v%. Place the mixture in a refrigerator at 4℃ and let it stand for 12 hours to allow the polysaccharide to precipitate completely. Discard the supernatant, then centrifuge to collect the precipitate. Add a small amount of 95 v / v% ethanol aqueous solution to wash the precipitate, and then centrifuge again. Repeat this process twice. Place the washed precipitate in a vacuum drying oven and dry it at 60℃ for 3 hours to obtain the Atractylodes lancea rhizome extract.

[0061] Preparation of rose water:

[0062] Fresh rose petals were mixed with deionized water at a mass-to-volume ratio of 1:26 g / mL and soaked for 2 hours. Then, the mixture was placed in an essential oil distillation extraction device and distilled for 3 hours using the material-water co-distillation method. Rose essential oil and rose hydrosol were separated by an oil-water separator. The rose hydrosol was then selected to obtain the rose water.

[0063] Oil-controlling and antioxidant composition

[0064] The raw materials were compounded and mixed according to the mass ratio in Table 1 to obtain oil-controlling and antioxidant compositions 1-4;

[0065] Table 1. Compositions for oil control and antioxidant effects

[0066] Raw material name Composition 1 Composition 2 Composition 3 Composition 4 Fermented extract of Prunus persica 5 7 9 11 Atractylodes rhizome extract 3 4 4 5 Centella Asiatica extract 0.5 0.6 0.7 0.8 rose water 55 60 60 69

[0067] The raw materials were mixed according to the mass ratio in Table 2 to obtain composition 5-8.

[0068] Table 2 Compositions 5-8

[0069] Raw material name Composition 5 Composition 6 Composition 7 Composition 8 Fermented extract of Prunus persica / 9 9 / Stone Peach Extract / / / 9 Atractylodes rhizome extract 4 / 4 4 Centella Asiatica extract 0.7 0.7 / 0.7 rose water 60 60 60 60

[0070] Note: " / " in the table indicates no addition.

[0071] Performance testing

[0072] 15α-Reductase Inhibition Rate Test

[0073] 1.1 Experimental Objectives and Principles

[0074] Oily skin has overactive sebaceous glands, resulting in a greasy face that is difficult to clean and has an unsightly shine. It is also prone to related skin conditions such as acne vulgaris and seborrheic dermatitis. Given the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of test samples on 5α-reductase is typically used to reflect the sebum-regulating effect, thus characterizing the oil-controlling efficacy of the test samples.

[0075] The results of the 5α-reductase inhibition rate test were compared between the test sample and the blank control. If the inhibition rate of the test sample was higher than that of the blank control and the difference was statistically significant (P < 0.05), the test sample could be considered to have a certain oil-controlling effect.

[0076] This test method is an in vitro method, suitable for evaluating cosmetics that claim to achieve oil control by inhibiting 5α-reductase.

[0077] 1.2 Test Indicators

[0078] Criteria for determining 5α-reductase inhibition rate: If the 5α-reductase inhibition rate of the sample is higher than that of the blank control and there is a significant difference (P<0.05), then the test sample can be considered to have a certain oil control effect. At the same time, the higher the inhibition rate and the more significant the difference (P<0.05) during the inter-group comparison, the better the oil control performance.

[0079] 1.3 Experimental Materials and Methods

[0080] 1.3.1 Test Materials

[0081] Testosterone, 98%; NADPH, 90%; Methanol, HPLC grade; Buffer: Tris-HCl;

[0082] 5α-Reductase crude extract: Liver tissue from 4 male SD rats was extracted at a tissue mass to buffer volume ratio of 1:2 g / mL to obtain crude enzyme extract.

[0083] 1.3.2 Test Methods

[0084] 1.3.2.1 Treatment of control materials and test samples

[0085] Test sample group: Dilute the above-prepared compositions 1-8 with buffer solution to a concentration of 5% for test sample solution;

[0086] Positive control: Finasteride solution was used as the positive control, with a test concentration of 0.93 mg / mL;

[0087] Blank control: Tris-HCl buffer.

[0088] 1.3.2.2 Test Operation Procedures

[0089] Sample tube A: Take 1 mL of sample solution into a test tube, add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 1 mL of testosterone solution, and mix well;

[0090] B Sample Base Tube: Take 1 mL of sample solution into a test tube, add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 1 mL of PBS solution, and mix well;

[0091] C solvent tube: Add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 1 mL of testosterone solution, add 1 mL of testosterone solution, and mix well;

[0092] D solvent base tube: Add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 2 mL of PBS solution, and mix well;

[0093] Each reaction solution was reacted at 37°C for 20 minutes. An appropriate amount of sample solution was then transferred and the absorbance was measured at 340 nm.

[0094] Each group has 3 parallel teams.

[0095] 1.3.2.3 Calculation Formula

[0096]

[0097] In the formula: A: represents the absorbance of the sample tube; B: represents the absorbance of the sample background tube; C: represents the absorbance of the solvent tube; D: represents the absorbance of the solvent background tube.

[0098] 1.3.2.4 Data Analysis

[0099] Statistical analysis was performed using SPSS 19.0. Independent samples t-tests or rank-sum tests were used for comparisons between groups. All statistical analyses were two-tailed tests, with a significance level of α = 0.05.

[0100] 1.3.3 Test Results

[0101] The results are shown in Table 3.

[0102] Table 35 α-Reductase Inhibition Rate

[0103] Note: In the table, "a" indicates a significant difference compared with the negative control group, P < 0.05, "aa" P < 0.01; "b" indicates a significant difference between composition groups 5-8 and composition group 3, P < 0.05, "bb" P < 0.01.

[0104] 1.3.3 Results Analysis

[0105] According to the experimental results, the inhibition rates of compositions 1-4 were significantly higher than those of the blank control group (P<0.01). Among them, composition 3 had the highest inhibition rate of 31.07%, which is close to 60% of the performance of the positive control group. This indicates that the compositions provided by the present invention have a strong inhibitory effect on 5α-reductase and can effectively inhibit the secretion of sebum in the skin, thereby achieving the effect of oil control.

[0106] Compositions 5-7 lacked the fermented extract of Prunus persica, the rhizome extract of Atractylodes lancea, and the extract of Centella asiatica, respectively, resulting in a significantly lower inhibition rate than composition 3 (P < 0.01). In particular, composition 5 had an inhibition rate of only 16.19%, indicating that the fermented extract of Prunus persica is the core active ingredient for oil control. This also proves that the fermented extract of Prunus persica, the rhizome extract of Atractylodes lancea, and the extract of Centella asiatica can exert a more superior oil control effect in rose water.

[0107] Composition 8 uses common *Pyrrosia lingua* extract instead of fermented extract, and its 5αR inhibition rate is significantly lower than that of composition 3 (P < 0.01), proving that *Aspergillus cristatus* fermentation can significantly enhance the efficacy of the active ingredients of *Pyrrosia lingua*.

[0108] 2 Antioxidant Test

[0109] 2.1 Experimental Grouping

[0110] The compositions 1-8 prepared above were diluted with deionized water to obtain test sample solutions 1-8 with a concentration of 5 wt%, and their antioxidant properties were tested. Details are shown in Table 4 below:

[0111] Table 4 Experimental group settings

[0112] Group Corresponding test substance Positive control group (VC) 100 μg / mL VC aqueous solution Sample solution 1 Solution containing 5% of composition 1 Sample solution 2 Solution containing 5% of composition 2 Sample solution 3 Solution containing 5% of composition 3 Sample solution 4 Solution containing 5% of composition 4 5 samples to be tested Solution containing 5% of composition 5 Sample solution 6 Solution containing 5% of composition 6 Sample solution 7 Solution containing 5% of composition 7 Sample solution 8 Solution containing 5% of composition 8

[0113] 2.2 Experimental Design

[0114] DPPH was prepared with anhydrous ethanol to a concentration of 2×10⁻⁶. -4 Take several portions of 2 mL each of the sample solution / VC, DPPH solution, and anhydrous ethanol. Mix 2 mL of the sample solution / VC and 2 mL of DPPH solution and let stand at room temperature for 30 min. Measure the absorbance at a wavelength of 517 nm to obtain Ai.

[0115] Mix 2 mL of the sample solution / VC and 2 mL of anhydrous ethanol, and then measure the absorbance as Aj according to the aforementioned method.

[0116] The absorbance was measured as Ac after mixing 2 mL of DPPH solution and 2 mL of deionized water according to the aforementioned method.

[0117] Each sample was measured in triplicate, and the average value was taken.

[0118] Calculate the scavenging rate of DPPH free radicals for each test sample according to the following formula (1) and record it in Table 5 below.

[0119] Clearance rate (%) = (1 - (Ai - Aj) / Ac) × 100%

[0120] In the formula: Ai is the absorbance of 2 mL of the sample solution to be tested / VC + 2 mL of DPPH solution mixture; Aj is the absorbance of 2 mL of the sample solution to be tested / VC + 2 mL of anhydrous ethanol mixture; Ac is the absorbance of 2 mL of DPPH solution + 2 mL of deionized water.

[0121] Table 5 DPPH removal rate

[0122] Group DPPH removal rate / % Positive control group 93.16 Sample solution 1 85.35 Sample solution 2 87.64 Sample solution 3 88.03 Sample solution 4 89.12 5 samples to be tested 61.52 Sample solution 6 65.33 Sample solution 7 58.48 Sample solution 8 75.10

[0123] Results analysis:

[0124] As shown in Table 5, the DPPH scavenging rates of compositions 1-4 all exceeded 85%, approaching the 93.16% of the positive control group (VC), demonstrating the excellent antioxidant capacity of the compositions provided by this invention. Composition 4 exhibited the highest scavenging rate, reaching 89.12%. The DPPH scavenging rates of compositions 5-7 were lower than that of composition 3, indicating that the absence of any one component in the system composed of *Prunus persica* fermentation extract, *Atractylodes lancea* rhizome extract, *Centella asiatica* extract, and rose water would lead to a decrease in the system's antioxidant properties.

[0125] Composition 8 uses common Prunus persica extract instead of fermented Prunus persica extract, resulting in a significantly lower scavenging rate than composition 3, further confirming that the fermentation process can enhance the antioxidant capacity of the active ingredients.

[0126] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition containing fermented extract of *Prunus persica*, characterized in that, The composition comprises, by weight parts, the following components: 5-11 parts of fermented extract of Prunus persica; 3-5 parts of Atractylodes lancea rhizome extract; Centella asiatica extract 0.5-0.8 parts; 55-69 parts rose water; The preparation method of the fermented extract of *Dendrobium nobile* includes the following steps: Step 1-1. First, activate and isolate *Aspergillus cristatus* to obtain a suspension of *Aspergillus cristatus* spores; Steps 1-2. Dry, pulverize, and sieve the pseudobulbs of *Dendrobium nobile* to obtain *Dendrobium nobile* powder; Steps 1-3. Mix the powdered *Dendrobium nobile*, glucose, dipotassium hydrogen phosphate, MgSO4·7H2O, vitamin B1 and sterile water to obtain a solid fermentation medium containing *Dendrobium nobile*. Sterilize the fermentation medium at 115℃ for 20 minutes before use. Steps 1-4. Take the *Aspergillus cristatus* spore suspension obtained in Step 1-1, and inoculate the bacterial suspension into a solid fermentation medium at 10-12% of the fermentation medium mass. Incubate at 28-30℃, aeration rate of 2-5 L / min·kg, and humidity of 70-80%RH for 5-7 days to obtain the fermentation material. Steps 1-5. Mix and soak the fermentation material with an ethanol aqueous solution of 60-70 v / v% at a mass-volume ratio of 1:20-25 g / mL. Treat the mixture with an ultrasonic frequency of 20-30 kHz and a temperature of 50-60℃ for 30-40 min. Centrifuge to separate the supernatant. Concentrate the supernatant under reduced pressure at 55-60℃ to 1 / 3 of the original volume to obtain the fermented extract of Prunus persica. The preparation method of the Atractylodes lancea rhizome extract includes the following steps: Step 2-1. Take dried Atractylodes rhizome, crush and sieve it to obtain Atractylodes powder. Mix Atractylodes powder with petroleum ether at a mass-volume ratio of 1:(8-10) g / mL, and reflux extract twice at 70-80℃ for 1.5h each time. After filtration, discard the filtrate and dry the resulting residue 1. Mix residue 1 with anhydrous ethanol at a mass-volume ratio of 1:(6-8) g / mL, and reflux extract three times at 70-80℃ for 1h each time. After filtration, discard the filtrate and dry the resulting residue 2 to obtain defatted Atractylodes powder. Step 2-2. Mix defatted Atractylodes lancea powder with deionized water at a mass-volume ratio of 1:(20-30)g / mL, soak at 80-90℃ and ultrasonic frequency of 28-30kHz for 30min, stop ultrasonication, keep at 80-90℃ for 3-4h for soaking and extraction, and centrifuge to obtain supernatant. Steps 2-3. Concentrate the supernatant under reduced pressure at 60-70℃ to 1 / 6 of its original volume to obtain a concentrated solution. Add 95 v / v% ethanol aqueous solution to the concentrated solution to adjust the final ethanol concentration to 70-80 v / v%. Place the mixture in a refrigerator at 4℃ and let it stand for 10-12 hours. Then centrifuge to separate the precipitate. Place the precipitate in a vacuum drying oven and dry it at 50-60℃ for 2-3 hours to obtain Atractylodes lancea rhizome extract. The method for preparing the rose water includes the following steps: Take fresh rose petals and soak them in deionized water at a mass-to-volume ratio of 1:(25-30) g / mL for 2-3 hours. Then place them in an essential oil distillation extraction device and distill them for 2.5-3.5 hours using the material-water co-distillation method. Separate the rose essential oil and rose hydrosol through an oil-water separator. Select the rose hydrosol to obtain the rose water. The Centella asiatica extract was purchased from Shandong Freda Biotechnology Co., Ltd.

2. The composition according to claim 1, characterized in that, The concentration of the *Eurotium cristatum* spore suspension mentioned in step 1-1 is 1 × 10⁻⁶. 9 ~5×10 9 cfu / mL.

3. The composition according to claim 1, characterized in that, In steps 1-2, the pseudobulbs of *Dendrobium nobile* are sliced, placed in a constant temperature drying oven and dried at 60°C to constant weight, and then pulverized in a high-speed pulverizer through a 60-mesh sieve to obtain *Dendrobium nobile* powder.

4. The composition according to claim 1, characterized in that, In steps 1-3, the powder of *Gynostemma pentaphyllum*, glucose, dipotassium hydrogen phosphate, MgSO4·7H2O, vitamin B1 and sterile water are mixed in a mass ratio of 100:(5-7):(1-2):(0.5-1):(0.01-0.02):(90-100).

5. The composition according to claim 1, characterized in that, The dried Atractylodes rhizome described in step 2-1 is pulverized and passed through a 30-40 mesh sieve to obtain Atractylodes powder.

6. The composition according to claim 1, characterized in that, In steps 2-3, the collected precipitate is washed with a small amount of 95 v / v% ethanol aqueous solution, and then centrifuged. This process is repeated twice.

7. The use of the composition according to any one of claims 1-6 in the preparation of skin care products with oil-controlling and antioxidant effects.

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