Protein detection chip and method

Abstract

The invention discloses a protein detection chip and method, the protein detection chip comprises a body, a first cover plate and a second cover plate, the body is cuboid, has a first surface and a second surface opposite to each other, and is provided with a self-circulation ventilation structure communicating the first surface with the second surface. A diluent storage tank, a diluent release tank, a sample adding tank, a sample separating tank, a mixing tank and a mixed liquid release tank which are sequentially arranged along the length direction of the body are arranged on a first surface of the body, a detection tank extending along the length direction of the body is arranged on a second surface of the body, and one end of the detection tank is communicated with the mixed liquid release tank through a transfer hole. The first cover plate covers the first surface of the body and is provided with a weak area corresponding to the sample adding groove; the second cover plate covers the second surface of the body, and the second cover plate is provided with a light-transmitting area corresponding to the detection groove. The protein detection chip provided by the invention has low requirements on a centrifugal instrument, so that the application scene can be broadened more easily.

Description

Technical Field

[0001] This application relates to the field of medical technology, and in particular to a protein detection chip and method. Background Technology

[0002] Microfluidics refers to the technology that integrates sample preparation, biochemical reactions, and detection processes in life sciences or chemistry onto a chip of only a few or tens of square centimeters. Combining microfluidics with protein detection can solve the challenges of clinical protein detection, such as reliance on large equipment and high personnel requirements.

[0003] Currently, the chips used in centrifugal microfluidics for protein detection are generally disk-shaped, with multiple chip units arranged around the center of the disk on the chip body, allowing for the simultaneous detection of multiple samples. However, these disk-shaped chips require centrifugation on specialized centrifuges, which places high demands on the equipment, makes it difficult to increase sample and indicator detection throughput, and results in high costs. Therefore, their application scenarios are limited and difficult to expand. Summary of the Invention

[0004] In view of this, one objective of this application is to provide a protein detection chip with lower requirements for centrifugation equipment, so that it can be easily extended to more application scenarios. Another objective of this application is to provide a protein detection method based on a protein detection chip.

[0005] This application provides the following technical solution:

[0006] A protein detection chip, comprising:

[0007] The body is rectangular in shape and has a first face and a second face. The first face is provided with a diluent storage tank, a diluent release tank, a sample addition tank, a sample separation tank, a mixing tank and a mixed solution release tank arranged sequentially along the length of the body. The second face is provided with a detection tank extending along the length of the body. One end of the detection tank is connected to the mixed solution release tank through a transfer hole. The body is provided with a self-circulating ventilation structure. The self-circulating ventilation structure includes a first air passage provided on the first face, a second air passage provided on the second face, and a vent hole that connects the first face and the second face. The first air passage and the second air passage are connected through the vent hole.

[0008] A first cover plate covers the first surface, and the first cover plate has a weak area corresponding to the sample dispensing slot;

[0009] A second cover plate covers the second surface, and the second cover plate has a light-transmitting area corresponding to the detection groove.

[0010] Optionally, in the protein detection chip described above, at least one waste liquid tank is provided on the first surface, and the first air passage is correspondingly connected to the diluent storage tank side of the waste liquid tank.

[0011] Optionally, the protein detection chip described above includes a cross-shaped mixing channel disposed on the first surface, the mixing channel being located between the sample separation tank and the mixing tank.

[0012] Optionally, the protein detection chip described above includes:

[0013] A mixing column is located inside the mixing tank;

[0014] Two mixing partitions are located inside the mixing tank and on the side of the mixing column near the mixing channel. The two mixing partitions are arranged in a figure-eight shape with their tips facing the mixing column. The ends of the two mixing partitions away from each other are connected to the inner wall of the mixing tank, and a gap is formed between the ends of the two mixing partitions close to each other.

[0015] Optionally, the protein detection chip described above includes at least two sample dispensing slots, which are arranged along the width direction of the body and are located between the sample separation slot and the mixing slot.

[0016] Optionally, the protein detection chip described above includes a first hydrophobic capillary channel extending in a serpentine shape on the first surface, with the two ends of the first hydrophobic capillary channel respectively connected to the sample dispensing tank and the mixing tank.

[0017] Optionally, in the above-mentioned protein detection chip, the diluent storage tank is equipped with a liquid storage box, the liquid storage box is provided with an easy-open strip for sealing the liquid outlet of the liquid storage chamber, and the first end face of the main body away from the mixed liquid release tank is provided with an avoidance notch for the easy-open strip to extend out.

[0018] Optionally, in the above-mentioned protein detection chip, the detection slot is filled with an immunochromatographic test strip; or,

[0019] The detection groove is equipped with a microcolumn array, and the surface of the microcolumns is coated with antibodies.

[0020] Optionally, in the above-mentioned protein detection chip, a positioning groove is formed on the second end face of the body near the end of the mixture release tank. The positioning groove is located at the intersection of the second end face and the first face, or at the intersection of the second end face and the second face; and / or,

[0021] The side of the main body away from the end of the mixture release tank is provided with a foolproof protrusion.

[0022] A protein detection method includes the following steps:

[0023] Step A: Take the protein detection chip, add the blood sample, the protein detection chip is the protein detection chip disclosed in any of the above, and the protein detection chip is pre-loaded with diluent. After adding the blood sample, seal the weak area of ​​the first cover corresponding to the sample application slot.

[0024] Step B: The protein detection chip is placed into the chip carrier of the centrifuge with the end closest to the mixture release tank facing down. The chip carrier is hinged to the rotatable rotating frame of the centrifuge, and the hinge axis is perpendicular to the rotation axis of the rotating frame.

[0025] Step C: Start the centrifuge and use centrifugal force to control the transfer of blood sample and diluent within the protein detection chip until the detection cell becomes detectable. Stop the centrifuge, as the optical signal to be detected exists in the detectable cell.

[0026] As can be seen from the above technical solution, the protein detection chip provided in this application has a roughly rectangular body shape, and the functional slots of the protein detection chip are arranged on both sides of the body. This design allows the protein detection chip to be centrifuged using most common centrifuges available in laboratory environments and simple medical settings, reducing the requirements for centrifuges and making it easier to broaden the application scenarios. In addition, the body has a self-circulating ventilation structure connecting the two sides, and the first and second cover plates cover the two sides of the body respectively, thereby achieving complete isolation between the fluid inside the chip and the outside air, avoiding problems such as fluid leakage and air pollution. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this application. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0028] Figure 1 This is an exploded view of a protein detection chip according to an embodiment of this application;

[0029] Figure 2 This is an assembly diagram of a protein detection chip according to an embodiment of this application;

[0030] Figure 3 yes Figure 1 A schematic diagram of the protein detection chip body shows the structure of the first side of the body;

[0031] Figure 4 yes Figure 1 A schematic diagram of the protein detection chip body from another perspective, showing the structure of the second side of the body;

[0032] Figure 5 yes Figure 1 An exploded view of the liquid reservoir of the protein detection chip in the image;

[0033] Figure 6 This is a schematic diagram illustrating the working principle of the pull tab on the liquid storage box of the protein detection chip;

[0034] Figure 7 This is a schematic diagram showing the arrangement of protein detection chips in a centrifuge;

[0035] Figure 8 Based on Figure 2 The flowchart of fluid manipulation for the protein detection chip shown is as follows;

[0036] Figure 9 This is a schematic diagram showing the arrangement of the protein detection chip inserted into the detector;

[0037] Figure 10 yes Figure 9 A schematic diagram of the internal structure of the detector in the image;

[0038] Figure 11 This is a schematic diagram of the body of a protein detection chip according to another embodiment of this application, showing the structure of the second side of the body;

[0039] Figure 12 This is an exploded view of a protein detection chip according to yet another embodiment of this application;

[0040] Figure 13 yes Figure 12 The diagram shows the assembly of the protein detection chip.

[0041] Figure 14 yes Figure 12 A schematic diagram of the protein detection chip body shows the structure of the first side of the body;

[0042] Figure 15 yes Figure 12 A schematic diagram of the protein detection chip body from another perspective, showing the structure of the second side of the body;

[0043] Figure 16 yes Figure 12 An exploded view of the liquid reservoir of the protein detection chip in the image;

[0044] Figure 17 Based on Figure 13 The flowchart shows the fluid manipulation process of the protein detection chip.

[0045] The diagram is marked as follows:

[0046] A. Protein detection chip; Z. Rotation axis; J. Hinge axis; Q. Diluent reagent;

[0047] 100. Body; 101. First hydrophilic capillary channel; 102. Second hydrophilic capillary channel; 103. Third hydrophilic capillary channel; 104. Fourth hydrophilic capillary channel; 105. Fifth hydrophilic capillary channel; 106. Sixth hydrophilic capillary channel; 107. Seventh hydrophilic capillary channel; 108. Eighth hydrophilic capillary channel; 109. Ninth hydrophilic capillary channel;

[0048] 110. Diluent storage tank; 120. Diluent release tank; 130. Sample addition tank; 140. Sample separation tank; 150. Mixing tank;

[0049] 111. Receiving groove; 112. Anti-foolproof protrusion; 113. Positioning groove;

[0050] 121. Diluent redistribution tank;

[0051] 131. First sample dispensing slot; 132. Second sample dispensing slot; 133. Third sample dispensing slot;

[0052] 141. Plasma storage tank; 142. Blood cell storage tank;

[0053] 151. First hydrophobic capillary channel; 152. Second hydrophobic capillary channel;

[0054] 161. First mixture distribution tank; 162. Second mixture distribution tank;

[0055] 171. First mixture release tank; 172. Second mixture release tank; 173. Sample release tank;

[0056] 181. First waste liquid tank; 182. Second waste liquid tank; 183. Third waste liquid tank; 184. Fourth waste liquid tank; 185. Fifth waste liquid tank;

[0057] 191. First airflow channel; 192. Second airflow channel; 193. Vent hole;

[0058] 201. First cover plate; 202. Second cover plate;

[0059] 300. Liquid storage box; 301. Easy-open strip; 302. Sealing film; 310. Box body; 311. Liquid filling port; 312. Vent port; 313. Protruding head; 314. Liquid outlet channel;

[0060] 400, absorbent pad; 500, immunochromatographic test strip; 600, interface valve;

[0061] 710. Mixing channel; 720. Mixing partition; 730. Mixing column;

[0062] 801, First detection slot; 802, Second detection slot; 810, Micropillar array;

[0063] 900. Detector; 901. Display screen; 902. Light source module; 903. Dichroic mirror; 904. Detector module; 905. Scanning module;

[0064] 910. Rotating frame; 920. Chip carrier; 930. Chip fixing fixture. Detailed Implementation

[0065] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0066] In the description of this application, the references to terms such as "one embodiment," "some embodiments," "example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of those different embodiments or examples.

[0067] In the description of this specification, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0068] See Figures 1-17This application provides a protein detection chip, mainly including a body 100, a first cover plate 201, and a second cover plate 202. The body 100 is rectangular and has a first and a second opposing surface. The first surface of the body 100 has a diluent storage tank 110, a diluent release tank 120, a sample loading tank 130, a sample separation tank 140, a mixing tank 150, and a mixed solution release tank arranged sequentially along the length of the body 100. The second surface of the body 100 has a detection tank extending along the length of the body 100, one end of which is connected to the mixed solution release tank via a transfer hole (not marked in the figure). The first cover plate 201 covers the first surface of the body 100 and has a weak area corresponding to the sample loading tank 130. The second cover plate 202 covers the second surface of the body 100 and has a light-transmitting area corresponding to the detection tank.

[0069] The body 100 is the main body of the protein detection chip. The main functional slots of the protein detection chip can be arranged along the length of the body 100. Furthermore, both sides of the body 100 in the thickness direction, namely the first and second sides mentioned above, can be used to set the functional slots of the protein detection chip. In other words, this application utilizes both sides of the body 100 to jointly arrange the functional slots of the protein detection chip. The first side of the body 100 can be used to set most of the functional slots of the protein detection chip. The main functional slots of the protein detection chip can include a diluent storage tank 110, a diluent release tank 120, a sample loading tank 130, a sample separation tank 140, a mixing tank 150, and a mixing solution release tank. These functional slots can be set on the first side of the body 100 and distributed along the length of the body 100. The second side of the body 100 can be used to set the detection slots of the protein detection chip. Simultaneously, the detection slots can communicate with the mixing solution release tank located at one end of the protein detection chip through transfer holes extending along the thickness direction of the protein detection chip.

[0070] This application, by designing the protein detection chip body 100 in a roughly cuboid shape and utilizing both sides of the body 100 to arrange the functional slots of the protein detection chip, allows the protein detection chip to be centrifuged using ordinary centrifuges, reducing the requirements for centrifuges and thus more easily expanding its application scenarios. In other words, this application, through optimized design of the protein detection chip's external shape and functional slot layout, enables it to better adapt to various types of centrifuges, thereby broadening its application scenarios.

[0071] The protein detection chip body 100 is used to control the fluid functions in each step. The fluid may include diluent reagent Q, the test sample, and a mixture of diluent reagent Q and the test sample. The diluent reagent Q can be stored in the diluent storage tank 110 during the production of the protein detection chip. That is, the diluent storage tank 110 is used to store the diluent reagent Q. It should be noted that the diluent reagent Q can be directly stored in the diluent storage tank 110, or it can be indirectly stored in the diluent storage tank 110 through another container (such as the liquid storage box 300 described later). That is, the diluent reagent Q is directly stored in another container, and the diluent storage tank 110 is used to accommodate the other container. The sample to be tested is added to the sample loading slot 130 of the body 100 through the weak area of ​​the first cover plate 201 when using the protein detection chip. The weak area of ​​the first cover plate 201 can be in various forms such as pores or thin films. It should be noted that after the sample to be tested is added to the sample loading slot 130 of the body 100, the weak area of ​​the first cover plate 201 needs to be sealed. For example, a small sealing film similar to an adhesive bandage can be used to seal the weak area of ​​the first cover plate 201.

[0072] The diluent reagent Q in the diluent storage tank 110 is released under centrifugal force and flows into the diluent release tank 120, which stores the diluent reagent Q after centrifugation. It should be understood that the diluent release tank 120 and the diluent storage tank 110 are interconnected via a liquid channel. The sample to be tested in the sample loading tank 130 is released under centrifugal force and flows into the sample separation tank 140. The sample separation tank 140 is used to separate the components of the sample to be tested through centrifugation. For example, the sample to be tested can be whole blood; under centrifugal force, the whole blood in the sample separation tank 140 is separated into blood cells and plasma.

[0073] Both the diluent reagent Q and the test sample flow into the mixing tank 150 for mixing. The mixture in the mixing tank 150 is released under centrifugal force and flows into the mixture release tank. It should be understood that the mixture release tank and the mixing tank 150 are interconnected via a liquid channel. Since the mixture release tank is connected to the detection tank on the second surface of the body 100 through a transfer hole, the mixture in the mixture release tank can flow into the detection tank through the transfer hole. It should be noted that the transfer hole is located at one end of the detection tank, and the other end of the detection tank extends along the length of the body 100 towards the side where the diluent storage tank 110 is located. That is, after the mixture flows into the detection tank through the transfer hole, it will flow in the detection tank in a direction opposite to the overall fluid flow direction on the first surface. See [link to relevant documentation]. Figures 1-4 In some embodiments, an absorbent pad 400 may be provided inside the body 100 of the protein detection chip. The absorbent pad 400 passes through the transfer hole to facilitate the transfer of fluid from the first side of the body 100 to the detection groove on the second side.

[0074] The detection slot, located on the second surface of the main body 100, is the site where detectable optical signals (e.g., fluorescence, chemiluminescence, visualization, etc.) are generated. Therefore, the detection slot is pre-set with antibodies capable of reacting with the test object. The second cover plate 202 has a light-transmitting area corresponding to the detection slot. This light-transmitting area allows the optical signals generated in the detection slot to be observed by personnel or detected by external devices (e.g., the detector 900 described later), thereby obtaining the measurement results of the measured index. In some embodiments, the second cover plate 202 can be entirely made of transparent material, making the area of ​​the second cover plate 202 corresponding to the detection slot a light-transmitting area. Of course, in other embodiments, the second cover plate 202 can be configured such that the area corresponding to the detection slot is made of transparent material to form a light-transmitting area, while other areas are made of non-transparent material, as long as it does not affect the personnel's observation of the optical signals generated in the detection slot or their detection by external devices.

[0075] See Figure 1 and Figure 12 In some embodiments, the detection groove may contain an immunochromatographic test strip 500, which is used to detect protein markers in the test subject and can generate a fluorescence signal. The two side walls of the detection groove may be provided with micro-protrusion structures (e.g., semi-cylindrical protrusions) as locking structures to fix the immunochromatographic test strip 500 and ensure that the test surface of the immunochromatographic test strip 500 is flat. Of course, in other embodiments, the immunochromatographic test strip 500 can be replaced by other methods, such as in… Figure 11 In the exemplary embodiment shown, a microcolumn array 810 may be disposed in the detection cell. The surface of the microcolumns of the microcolumn array 810 is coated with antibodies. The microcolumn array 810 is used to detect protein markers in the test object and can generate fluorescence signals.

[0076] See Figure 4 , Figure 11 and Figure 15 In some embodiments, there can be two or more detection slots, which can simultaneously detect multiple indicators. That is, each detection slot can correspond to different indicators. For example, different detection slots can be filled with immunochromatographic test strips 500 that detect different indicators, thereby using a protein detection chip to detect multiple indicators.

[0077] See Figure 1 , Figure 3 , Figure 5 and Figure 6In some embodiments, the diluent storage tank 110 may house a storage box 300. The storage box 300 may be provided with a pull tab 301 for sealing the outlet of the storage chamber. A clearance notch may be provided on the first end face of the body 100 at the end furthest from the mixture release tank for the pull tab 301 to extend from. The storage box 300 is used to store the diluent reagent Q, and the diluent storage tank 110 is used to accommodate the storage box 300. Figure 6 As shown, when using the protein detection chip, by pulling the easy-pull strip 301 on the liquid storage box 300, the liquid storage chamber of the liquid storage box 300 can be changed from a closed state to an open state. In the open state, the liquid storage chamber of the liquid storage box 300 is connected to the liquid outlet channel 314, that is, the diluent reagent Q in the liquid storage chamber can flow into the liquid outlet channel 314 through the liquid outlet and then flow out of the liquid storage box 300.

[0078] See Figure 6 In some embodiments, the pull tab 301 can seal the liquid storage cavity of the liquid storage box 300, and after being folded at the connection between the liquid storage cavity and the liquid outlet channel 314, it extends outward from the liquid storage box 300. That is, the pull tab 301 is bent into a C-shape, with a portion remaining outside the liquid storage box 300 for pulling. This arrangement makes it easier to tear a liquid outlet at the connection between the liquid outlet channel 314 and the liquid storage cavity by pulling the pull tab 301. Of course, in other embodiments, the pull tab 301 may not be folded, but may extend directly outward from the liquid storage box 300, as long as a liquid outlet can be torn out by pulling the pull tab 301 to change the liquid storage cavity from a closed state to an open state. It should be noted that this application does not limit the material of the pull tab 301. In order to reduce the impact on the diluent reagent Q, the pull tab 301 may be made of, for example, aluminum foil.

[0079] See Figure 5 In some embodiments, the liquid storage box 300 may include a box body 310 and a sealing film 302. The box body 310 may have a filling port 311 and a venting port 312 communicating with the internal liquid storage chamber. The sealing film 302 is used to seal the filling port 311 and the venting port 312 after the diluent reagent Q is added to the liquid storage box 300. The sealing film 302 can be fixed to the box body 310 by means of single-sided adhesive, heat sealing film, or other connection methods. It should be understood that the filling port 311 is used to add the diluent reagent Q into the box body 310, and the venting port 312 is used to expel gas from the liquid storage chamber during the process of adding the diluent reagent Q. In some embodiments, one end of the housing 310 may be provided with a protrusion 313, and the portion of the liquid storage cavity inside the housing 310 corresponding to the protrusion 313 forms a converging structure toward the diluent release tank 120 (for example, it may be a flow guide surface in the form of an arc or a stepped shape, which serves to collect the fluid to the outlet), thereby promoting the diluent reagent Q to be completely drained from the liquid storage cavity and enter the diluent release tank 120 under the action of centrifugal force.

[0080] exist Figures 1-5 In the exemplary embodiment shown, the protein detection chip is provided with a reservoir 300. In other embodiments, the number of reservoirs 300 can be set to other values, for example in... Figures 12-16 In the exemplary embodiment shown, the protein detection chip is provided with two reservoirs 300. When there is more than one reservoir 300, different reservoirs 300 can hold different diluent reagents Q, thereby meeting more detection needs. See also Figure 3 and Figure 14 To facilitate pulling the pull tab 301, the bottom of the diluent storage tank 110 may have a receiving groove 111 for accommodating the pull tab 301. The receiving groove 111 can better prevent jamming when pulling the pull tab 301. The receiving groove 111 is located at the bottom of the diluent storage tank 110 and extends to the clearance notch on the first end face of the body 100 for the pull tab 301 to extend out. It should be noted that the depth of the receiving groove 111 should not be too large, just enough to allow the movement of the pull tab 301 to be unrestricted.

[0081] See Figure 3 In some embodiments, the protein detection chip may include a cross-shaped mixing channel 710 disposed on the first surface of the body 100, located between the sample separation tank 140 and the mixing tank 150. By providing the cross-shaped mixing channel 710, a more thorough mixing effect between the first and second reaction solutions can be achieved; that is, the diluent reagent Q and the sample to be tested are mixed in the mixing channel 710 before flowing into the mixing tank 150. In some embodiments, the diluent release tank 120 can be connected to the inlet end of the mixing channel 710 via a first hydrophilic capillary channel 101 disposed on the first surface of the body 100, and the sample separation tank 140 can be connected to the inlet end of the mixing channel 710 via a second hydrophilic capillary channel 102 disposed on the first surface of the body 100. The hydrophilic capillary channel can utilize capillary force and centrifugal force to transfer fluids. It should be understood that the hydrophilic capillary channel is a capillary channel treated with a hydrophilic reagent, including but not limited to Tween and Triton. The capillary channel has a small width and depth, enabling it to passively drive fluid transfer with sufficient capillary force. An interface valve 600 can be installed on the hydrophilic capillary channel. The interface valve 600 acts as a passive valve to prevent fluid transfer within the hydrophilic capillary channel when the centrifugal force is insufficient. The interface valve 600 primarily prevents further liquid flow by increasing the surface area of ​​the capillary channel and utilizing liquid surface tension. This application does not limit the shape or structure of the interface valve 600; its shape can be circular, square, triangular, etc.

[0082] It should be noted that the capillary tube is used to transfer fluid from the upper functional tank to the lower functional tank. When setting up the capillary tube for transferring fluid, considering that the capillary tube and the upper functional tank to which it is connected form a communicating vessel structure, in order to prevent the fluid upstream of the upper functional tank from bypassing the capillary tube and directly entering the lower functional tank under the action of centrifugal force, the capillary tube should be set to start from the upper functional tank, extend in the opposite direction of centrifugal force and pass beyond the upper functional tank, and then bend towards the lower functional tank. Taking the first hydrophilic capillary as an example, the first hydrophilic capillary is used to transfer fluid from the diluent release tank 120 to the mixing channel 710. Therefore, after starting from the diluent release tank 120, the first hydrophilic capillary first extends along the length direction of the body 100 towards the side where the diluent storage tank 110 is located, and extends beyond the diluent release tank 120. Then it bends and extends back, extending along the length direction of the body 100 towards the side where the mixing channel 710 is located. In this way, in the step of the fluid entering the diluent release tank 120 from the diluent storage tank 110, the fluid will not pass through the capillary under the action of centrifugal force and directly enter the mixing channel 710.

[0083] Based on the aforementioned mixing channel 710, the protein detection chip may include a mixing column 730 and two mixing partitions 720 located within the mixing tank 150. (See also...) Figure 3 The mixing partitions 720 are located on the side of the mixing column 730 near the mixing channel 710. The two mixing partitions 720 are arranged in a V-shape with their tips facing the mixing column 730. The ends of the two mixing partitions 720 furthest from each other are connected to the inner wall of the mixing tank 150, while a gap is formed between the ends of the two mixing partitions 720 closest to each other. The diluent reagent Q and the test sample flow through the mixing channel 710 and into the mixing tank 150. They first flow in the space formed by the two V-shaped mixing partitions 720 and the inner wall of the mixing tank 150, and then flow through the gap between the two mixing partitions 720 towards the mixing column 730, where they are further mixed by the turbulence caused by the mixing column 730. By setting the mixing column 730 and mixing partitions 720 within the mixing tank 150, a more thorough mixing effect can be achieved, which is beneficial for improving the reliability of the test results.

[0084] See Figure 3 In some embodiments, the protein detection chip may include at least two mixing solution dispensing slots arranged along the width direction of the body 100, located between the mixing slot 150 and the mixing solution release slot. Figure 3 and Figure 4In the exemplary embodiment, the first surface of the body 100 is provided with a first mixture distribution tank 161 and a second mixture distribution tank 162 arranged side by side. Both are connected to a mixing tank 150 via a third hydrophilic capillary channel 103. Furthermore, the first mixture distribution tank 161 is connected to a first mixture release tank 171 via a fourth hydrophilic capillary channel 104, and the second mixture distribution tank 162 is connected to a second mixture release tank 172 via a fifth hydrophilic capillary channel 105. Transfer holes are provided between the first mixture release tank 171 and the first detection tank 801 on the second surface of the body 100, and between the second mixture release tank 172 and the second detection tank 802 on the second surface of the body 100. By providing the mixture distribution tanks, the mixture can be quantitatively distributed into the first mixture distribution tank 161 and the second mixture distribution tank 162, and then transferred to the first mixture release tank 171 and the second mixture release tank 172, respectively.

[0085] See Figure 3 In some embodiments, the protein detection chip may include a waste liquid tank disposed on a first side of the body 100. The waste liquid tank is connected to other functional tanks and is used to store excess liquid, ensuring that the volume of liquid in the functional tanks connected to the waste liquid tank is quantitatively measured. The number and location of the waste liquid tanks can be set as needed, for example, in... Figure 3 In the exemplary embodiment, the first surface of the body 100 is provided with a first waste liquid tank 181 connected to the diluent release tank 120, a second waste liquid tank 182 connected to the sample separation tank 140, a third waste liquid tank 183 connected to the mixing tank 150, and a fourth waste liquid tank 184 connected to the second mixture distribution tank 162. The waste liquid tanks and their connected functional tanks are arranged along the width direction of the body 100, thereby making fuller and more reasonable use of the area of ​​the first surface of the body 100.

[0086] See Figure 3 and Figure 4In some embodiments, the body 100 of the protein detection chip may be provided with a self-circulating ventilation structure. This structure includes a first ventilation channel 191 disposed on the first surface of the body 100 and a second ventilation channel 192 disposed on the second surface of the body 100. The first and second ventilation channels 191 are connected by a vent 193, meaning the vent 193 extends along the thickness direction of the body 100 through both the first and second surfaces. This self-circulating ventilation structure can achieve internal pressure balance within the protein detection chip, using a roundabout air supply to offset the positive and negative pressure differences generated during fluid transfer. In other words, the self-circulating ventilation structure formed by the first ventilation channel 191, the vent 193, and the second ventilation channel 192 eliminates the need for external air supply during fluid transfer, thus achieving complete isolation between the internal fluid and external air, avoiding fluid leakage and air pollution. Essentially, the inside and outside of the protein detection chip are not ventilated. See [link to relevant documentation]. Figure 1 and Figure 2 The first cover plate 201 can seal the first surface of the body 100, and the second cover plate 202 can seal the second surface of the body 100. The internal space formed by the self-circulating ventilation structure, the functional slots of the protein detection chip, and the capillary channels is not connected to the outside. During the fluid transfer process, the pressure balance is achieved solely by the gas flow in the internal space to offset the positive and negative pressure differences generated during fluid transfer, thereby ensuring smooth fluid transfer. The number and specific location of the first ventilation channel 191, the vent 193, and the second ventilation channel 192 can be flexibly set as needed. On the first surface of the body 100, the vent 193 should be located on the side of the functional slot connected to it in the opposite direction of centrifugal force, so that the fluid will not flow from the functional slot into the vent 193 under the action of centrifugal force.

[0087] The function of the self-circulating ventilation structure is to ensure that the air pressure between the two functional slots is balanced when the fluid is transferred between them, thus ensuring the smooth transfer of the fluid. On the first side of the main body 100, a first air passage 191 can be connected to each of the two functional slots on the side of the diluent storage tank 110 (i.e., the side facing the diluent storage tank 110). Alternatively, if the two functional slots are equipped with corresponding waste liquid tanks, a first air passage 191 can be connected to the side of the waste liquid tank on the side of the diluent storage tank 110. When setting the first air passage 191 and the second air passage 192, the air passage can be curved on the surface of the body 100. The curve trajectory of the air passage is generally set as a monotonic curve relative to the length direction of the body 100. That is, in a plane coordinate system with the length direction of the body 100 as the independent variable coordinate axis and the width direction of the body 100 as the dependent variable coordinate axis, the extension trajectory of the air passage is a monotonically increasing or monotonically decreasing curve. In this way, even if a small amount of liquid accidentally enters the air passage, it can flow more easily into the downstream functional tank under the action of centrifugal force, reducing the risk of liquid clogging the air passage.

[0088] See Figure 7 The protein detection chip A provided in this application can be fluid-manipulated on a simple centrifuge. In some embodiments, the centrifuge may include a rotating frame 910, a chip carrier 920, and a chip fixing clamp 930. The chip carrier 920 is hinged to the rotatable rotating frame 910 of the centrifuge, and the hinge axis J is perpendicular to the rotation axis Z of the rotating frame 910. The chip carrier 920 is provided with a bearing cavity for mounting the chip fixing clamp 930. The chip carrier 920 may have two or more bearing cavities. The chip fixing clamp 930 is provided with a limiting groove for mounting the protein detection chip A. The chip fixing clamp 930 may have two or more limiting grooves. Figure 7 As shown, when the centrifuge is not started, the chip carrier 920 is in an upright position under the action of gravity, and the protein detection chip A is inserted into the limiting groove of the chip fixing clamp 930 with the end near the mixing liquid release tank facing downwards. After the centrifuge is started, as the rotating frame 910 rotates, the chip carrier 920 will rotate around the hinge axis J to a certain tilt state. The greater the rotation speed of the rotating frame 910, the greater the tilt angle of the chip carrier 920 relative to the vertical direction. Of course, in other embodiments, the chip fixing clamp 930 may not be provided, and instead, a limiting groove for mounting the protein detection chip A may be provided on the chip carrier 920, that is, the protein detection chip A can be directly fixed on the chip carrier 920.

[0089] The rotating frame 910 can have various structural options; see [link / reference]. Figure 7In some embodiments, the rotating frame 910 may include a plurality of fork-shaped hinges evenly distributed around the rotation axis Z, and one end of the chip carrier 920 may be located in a notch of the hinge and hinged to the hinge. The chip carriers 920 connected to each hinge may be the same or different, for example, in… Figure 7 In the centrifuge on display, four chip carriers 920 are connected to the rotating rack 910. Two of the chip carriers 920 are identical, each having two housing chambers for mounting the chip clamps 930. The other two chip carriers 920 have more housing chambers. It is easy to understand that the more housing chambers, the more protein detection chips A the centrifuge can simultaneously manipulate.

[0090] The following is based on Figure 8 Example introduction Figures 1-5 The working principle of the protein detection chip shown, the fluid manipulation process is as follows: Figure 8 As shown in parts (1) to (7) of the figure, it should be noted that the labels ① and ② in the figure are used to indicate the current position of the fluid. The centrifugal shaft in the figure refers to the rotation axis Z of the centrifuge's rotating frame 910. Figure 8 Parts (1) to (6) of the document show the first face of the main body 100. Figure 8 Part (7) shows the second side (i.e. the back) of the main body 100.

[0091] See Figure 8 In part (1), the first reaction solution (i.e., diluent reagent Q) is loaded at point ①, and the sample is added at point ② (i.e., whole blood sample is injected into sample tank 130). The reagent loading procedure can be found in [reference needed]. Figure 6 The diluent reagent Q is loaded by pulling the pull strip 301. Sample injection is performed by injecting the sample into the sample loading chamber 130 through the weak area of ​​the first cover 201 using an injection device (e.g., a syringe), and then sealing the weak area of ​​the first cover 201 (using a film or other method). After reagent loading and sample injection are completed, the protein detection chip is placed in a centrifuge, and the centrifuge is started.

[0092] See Figure 8 In step (2), the centrifuge performs high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm. The diluent reagent Q and the whole blood sample are driven by centrifugal force. The diluent reagent Q is transferred to the position shown in ①, that is, the diluent release tank 120. The whole blood sample is transferred to the position shown in ②, that is, the sample separation tank 140. The whole blood sample is separated into a lower layer of blood cell sediment that enters the blood cell storage tank 142 and an upper layer of plasma that enters the plasma storage tank 141.

[0093] See Figure 8In part (3), after completing the pre-centrifugation step, let it stand briefly to allow the diluent reagent Q and plasma to fill the capillary channel with the help of siphon force. Then, start the centrifuge again for low-speed centrifugation for 20 seconds. The low speed can be, for example, 1000 rpm. The diluent reagent Q and plasma are driven by centrifugal force to transfer to the position shown in ①, i.e., the mixing tank 150.

[0094] See Figure 8 In part (4), after completing the pre-centrifugation step, the mixture is allowed to stand briefly to fill the capillary channel with the help of siphon force. The centrifuge is then started again for high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm. The mixture is driven by centrifugal force to transfer to the position shown in ①, namely the first mixture distribution tank 161 and the second mixture distribution tank 162.

[0095] See Figure 8 In part (5), after completing the pre-centrifugation step, the mixture is allowed to stand briefly to fill the capillary channel with the help of siphon force. The centrifuge is then started again for high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm. The mixture in the first mixture distribution tank 161 and the second mixture distribution tank 162 is transferred to the position shown in ① by centrifugal force, that is, the first mixture release tank 171 and the second mixture release tank 172.

[0096] See Figure 8 In part (6) of the above, after completing the pre-centrifugation step, the mixture is allowed to stand briefly to stabilize, and then the centrifuge is restarted for high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm, so that the mixture is loaded onto the absorbent pad 400 by centrifugal force and transferred from the first side of the body 100 to the second side (i.e., the back side) of the body 100 by the suction of the absorbent pad 400. It should be noted that this step can also be performed without centrifugation, and the absorbent pad 400 can also slowly transfer the mixture by suction. Of course, in some embodiments, the protein detection chip may not have the absorbent pad 400, and the mixture can also flow through the transfer hole on the body 100 to the immunochromatographic test strip 500 placed in the detection tank under the action of centrifugal force.

[0097] See Figure 8In part (7), after the pre-centrifugation step, the mixture comes into contact with the immunochromatographic test strip 500 and flows slowly on the immunochromatographic test strip 500. It should be noted that this step can be performed by starting the centrifuge at a very low speed, such as 200 rpm, so that the mixture is subjected to a small centrifugal force in the opposite direction, thereby slowing down the flow rate of the mixture on the immunochromatographic test strip 500, increasing its reaction time with the coated antibody on the test strip, and thus improving the reaction sensitivity. In other words, the centrifugation operation is for reaction sensitization. Of course, in some embodiments, this centrifugation operation may not be performed.

[0098] See Figure 9 After the protein detection chip A completes fluid manipulation on the centrifuge, it can be removed from the centrifuge. The fluorescence signal is then detected by the detection optical path of the detector 900 through the light-transmitting area of ​​the second cover plate 202, thus completing the detection of the immune marker. During operation, the end of the protein detection chip A closest to the mixing solution release tank is inserted into the chip slot of the detector 900, and then the detector 900 is started. The detector 900 has a display screen 901, on which the detection results can be displayed. It should be noted that the working principle of the detector 900 is based on existing technology; reference to conventional detectors is sufficient, and it will not be described in detail here.

[0099] See Figure 10 In some embodiments, the main components of the detector 900 may include a light source module 902, a dichroic mirror 903, and a detector module 904. It should be noted that the number of these main components of the detector 900 can be set as needed. Figure 10 The detector 900 shown as an example is suitable for Figures 12-16 The protein detection chip shown has three detection slots, so the detector 900 contains three independent optical paths. Moreover, the three optical paths in the detector 900 are arranged in a triangular pattern to avoid crosstalk between adjacent optical paths.

[0100] In some embodiments, the surface of the second cover plate 202 of the protein detection chip may be provided with an identifier containing information, such as a barcode or QR code. The information stored in the identifier may include the batch number of the immunochromatographic test strip 500 contained in the protein detection chip, the immunoassay indicators used by the protein detection chip for detection, and other information. In order to read the information stored in the identifier on the second cover plate 202, the detector 900 may include a scanning module 905, which scans the barcode, QR code, or other identifier to obtain the information stored in the identifier.

[0101] See Figure 3In some embodiments, a foolproof protrusion 112 may be provided on the side of the body 100 at the end away from the mixing solution release tank. The body 100 is generally rectangular in shape, and the foolproof protrusion 112 makes the width of this end of the body 100 larger than the width of other parts of the body 100. Thus, when the protein detection chip A... Figure 7 When placed into the limiting slot as shown, or as... Figure 9 When the protein detection chip is inserted into the slot of the detector 900 as shown, reverse insertion is prevented, thus achieving a foolproof effect. In other words, the foolproof protrusion 112 ensures that the end of the protein detection chip furthest from the mixing solution release tank is inserted inwards, thereby guaranteeing correct placement of the protein detection chip. The structure of the foolproof protrusion 112 can be varied; for example, it can be triangular, square, trapezoidal, semi-circular, etc. Furthermore, the body 100 can be as follows... Figure 7 As shown, anti-foolproof protrusions 112 are provided on both opposite sides, or anti-foolproof protrusions 112 can be provided only on one side.

[0102] In some embodiments, a positioning groove 113 may be formed on the second end face of the body 100 near the end of the mixture release tank. The positioning groove 113 may be located at the intersection of the second end face and the first face, or at the intersection of the second end face and the second face. See also Figure 9 and Figure 10 After the protein detection chip A is placed into the slot of the detector 900, the second cover plate 202 of the protein detection chip A must be aligned with the detector module 904 of the detector 900. The positioning slot 113 of the body 100 can be used to cooperate with the positioning structure inside the detector 900, so that the protein detection chip A can only be inserted into place when the second cover plate 202 is aligned with the detector module 904 of the detector 900. This ensures that the inserted protein detection chip A meets the requirement of the detector 900 for the orientation of the second cover plate 202. For example, a marking line can be provided on the surface of the end of the body 100 away from the positioning groove 113. After the protein detection chip A is inserted into the detector 900, the marking line is flush with the slot opening of the detector 900. If the second cover 202 of the protein detection chip A is reversed (i.e., it does not meet the requirement of the detector 900 for the orientation of the second cover 202), then due to the presence of the positioning groove 113, the positioning structure inside the detector 900 that cooperates with the positioning groove 113 will push against the end face of the protein detection chip A, so that the protein detection chip A cannot be inserted further before the marking line reaches the position flush with the slot opening of the detector 900. In other words, the insertion of the protein detection chip A is restricted, thereby ensuring that the protein detection chip A can only be inserted when the second cover 202 is facing the detector module 904 of the detector 900.

[0103] See Figures 12-16In some embodiments, the protein detection chip may include at least two sample dispensing slots arranged along the width of the body 100, located between the sample separation slot 140 and the mixing slot 150. Figure 14 In the exemplary embodiment, the first surface of the body 100 is provided with three sample distribution slots, namely a first sample distribution slot 131, a second sample distribution slot 132, and a third sample distribution slot 133. A sample separation slot 140 is connected to the sample separation slot 140 via an eighth hydrophilic capillary channel 108. Whole blood samples are separated into blood cells and plasma in the sample separation slot 140. The plasma then flows into the three sample distribution slots, thus being separated into three plasma samples, enabling multi-index detection on a single chip. In some embodiments, the first surface of the body 100 may be provided with a fifth waste liquid tank 185 connected to the sample distribution slots, for example, in... Figure 14 In the structure shown, the fifth waste liquid tank 185 and each sample distribution tank are arranged along the width direction of the body 100, and the third sample distribution tank 133 is connected to the fifth waste liquid tank 185.

[0104] In some embodiments, the first surface of the body 100 may be provided with a first hydrophobic capillary channel 151 extending in a serpentine shape, the two ends of the first hydrophobic capillary channel 151 being connected to a sample dispensing tank and a mixing tank 150, respectively. See also Figure 14 The main body 100 is provided with two mixing tanks 150. One mixing tank 150 is connected to the first sample distribution tank 131 through a first hydrophobic capillary channel 151, and the other mixing tank 150 is connected to the third sample distribution tank 133 through another first hydrophobic capillary channel 151. The two mixing tanks 150 are respectively connected to the first mixture release tank 171 and the second mixture release tank 172 through their respective ninth hydrophilic capillary channels 109. It should be understood that the hydrophobic capillary channel is a capillary channel treated with a hydrophobic reagent. The first hydrophobic capillary channel 151 is S-shaped, which can increase the flow resistance, so that the fluid can only enter the mixing tank 150 through the first hydrophobic capillary channel 151 under the centrifugal action of relatively high speed.

[0105] In some embodiments, the first surface of the body 100 may be provided with a sample release groove 173 and a second hydrophobic capillary channel 152 extending in a serpentine shape. The two ends of the second hydrophobic capillary channel 152 are respectively connected to the sample release groove 173 and one of the sample dispensing grooves, for example, in Figure 14 In the illustrated structure, the second sample dispensing tank 132 is connected to the sample release tank 173 via a second hydrophobic capillary channel 152. (See also...) Figure 14 and Figure 15The second surface of the main body 100 can be provided with three detection slots, namely a first detection slot 801, a second detection slot 802, and a third detection slot 803. Transfer holes are provided between the first mixed solution release slot 171 and the first detection slot 801, between the second mixed solution release slot 172 and the second detection slot 802, and between the sample release slot 173 and the third detection slot 803. The sample release slot 173 is used for the detection of immunoassay indicators using the stock solution method; that is, the sample release slot 173 contains a plasma sample that has not been mixed with the diluent reagent Q. After flowing out of the sample release slot 173, the plasma sample directly reaches the third detection slot 803 through the transfer hole.

[0106] In some embodiments, the first mixture release tank 171, the second mixture release tank 172, and the sample release tank 173 are arranged in a triangular pattern. On the second side of the body 100, the first detection tank 801, the third detection tank 803, and the second detection tank 802 are arranged sequentially along the width direction of the body 100. The third detection tank 803, located in the middle, is not aligned with the ends of the other two detection tanks, but is misaligned along the length direction of the body 100. This avoids the three independent optical paths corresponding to the first detection tank 801, the third detection tank 803, and the second detection tank 802 in the detector 900 from being too close to each other, which helps to avoid crosstalk between adjacent optical paths.

[0107] See Figure 14 In some embodiments, two diluent storage tanks 110, two diluent release tanks 120, and two mixing tanks 150 can be provided. This arrangement allows for the separate processing of the sample using different diluent reagents Q, thereby enabling the detection of more immune indicators. The two diluent storage tanks 110 are distributed along the width direction of the body 100, the two diluent release tanks 120 are distributed along the width direction of the body 100, and the two mixing tanks 150 are distributed along the width direction of the body 100. The first surface of the body 100 may be provided with two diluent redistribution tanks 121 distributed along the width direction of the body 100, located between the sample loading tank 130 and the diluent release tanks 120. The two diluent redistribution tanks 121 are connected to the diluent release tank 120 through their respective sixth hydrophilic capillary channels 106, and to the mixing tank 150 through their respective seventh hydrophilic capillary channels 107. The diluent redistribution tank 121 is used to perform secondary quantitative distribution of diluent reagent Q to match the processing steps of the sample being tested.

[0108] See Figure 15In some embodiments, the portion of the self-circulating ventilation structure located on the second surface of the body 100 (i.e., the second ventilation channel 192) may be disposed at the bottom of the detection slot. Furthermore, the self-circulating ventilation structure may include connecting different second ventilation channels 192. For example, the second surface of the body 100 may be provided with a second ventilation channel 192 extending along the width direction of the body 100, connecting the first detection slot 801, the second detection slot 802, and the third detection slot 803.

[0109] The following is based on Figure 17 Example introduction Figures 12-16 The working principle of the protein detection chip shown, the fluid manipulation process is as follows: Figure 17 As shown in parts (1) to (7) of the figure, it should be noted that the labels ① and ② in the figure are used to indicate the current position of the fluid. The centrifugal shaft in the figure refers to the rotation axis Z of the centrifuge's rotating frame 910. Figure 17 Parts (1) to (6) of the document show the first face of the main body 100. Figure 17 Part (7) shows the second side (i.e. the back) of the main body 100.

[0110] See Figure 17 In part (1), the first reaction solution (i.e., diluent reagent Q) is loaded at point ①, and the sample is added at point ② (i.e., whole blood sample is injected into sample tank 130). The reagent loading procedure can be found in [reference needed]. Figure 6 The diluent reagent Q is loaded by pulling the pull strip 301. Sample injection is performed by injecting the sample into the sample loading chamber 130 through the weak area of ​​the first cover 201 using an injection device (e.g., a syringe), and then sealing the weak area of ​​the first cover 201 (using a film or other method). After reagent loading and sample injection are completed, the protein detection chip is placed in a centrifuge, and the centrifuge is started.

[0111] See Figure 17 In step (2), the centrifuge performs high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm. The diluent reagent Q and the whole blood sample are driven by centrifugal force. The diluent reagent Q is transferred to the position shown in ①, that is, the diluent release tank 120. The whole blood sample is transferred to the position shown in ②, that is, the sample separation tank 140. The whole blood sample is separated into a lower layer of blood cell sediment that enters the blood cell storage tank 142 and an upper layer of plasma that enters the plasma storage tank 141.

[0112] See Figure 17In part (3), after completing the pre-centrifugation step, allow the sample to stand briefly to allow the diluent reagent Q and plasma to fill the capillary channel using siphon force. Then, restart the centrifuge for low-speed centrifugation for 20 seconds. The low speed can be, for example, 1000 rpm. The diluent reagent Q is driven by centrifugal force to the position shown in ①, i.e., the diluent redistribution tank 121, and the plasma sample is driven by centrifugal force to the position shown in ②, i.e., the sample distribution tank. It should be noted that the low-speed centrifugation here is to prevent the plasma sample from being subjected to excessive centrifugal force, which could cause it to break through the hydrophobic capillary channel below the sample distribution tank. The specific speed varies depending on the centrifuge radius.

[0113] See Figure 17 In part (4), after completing the pre-centrifugation step, the sample is briefly allowed to stand to allow the diluent reagent Q and plasma to fill the capillary channel using siphon force. The centrifuge is then restarted for high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm. The diluent reagent Q and plasma sample are driven by centrifugal force to transfer to the position shown in ①, i.e., the mixing tank 150. It should be noted that in this embodiment, the target index of the plasma in one of the sample distribution tanks (i.e., the second sample distribution tank 132) does not need to be mixed with the diluent reagent Q (stock solution method detection), so it directly enters the sample release tank 173 through the second hydrophobic capillary channel 152. It should be noted that the high-speed centrifugation here is to allow the plasma in the sample distribution tank to break through the hydrophobic capillary channel below and enter the next functional tank. The specific speed varies depending on the centrifuge radius.

[0114] See Figure 17 In part (5), after completing the pre-centrifugation step, the mixture is allowed to stand briefly to fill the capillary channel with the help of siphon force. The centrifuge is then restarted for high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm. The mixture in the two mixing tanks 150 is transferred to the positions shown in ① by centrifugal force, namely the first mixture release tank 171 and the second mixture release tank 172. The plasma in the sample release tank 173 is transferred to the second side (i.e. the back side) of the body 100 by centrifugal force through the absorbent pad 400.

[0115] See Figure 17In part (6) of the above, after completing the pre-centrifugation step, the mixture is allowed to stand briefly to stabilize, and then the centrifuge is restarted for high-speed centrifugation for 20 seconds. The high speed can be, for example, 2000 rpm, so that the mixture is loaded onto the absorbent pad 400 by centrifugal force and transferred from the first side of the body 100 to the second side (i.e., the back side) of the body 100 by the suction of the absorbent pad 400. It should be noted that this step can also be performed without centrifugation, and the absorbent pad 400 can also slowly transfer the mixture by suction. Of course, in some embodiments, the protein detection chip may not have the absorbent pad 400, and the mixture can also flow through the transfer hole on the body 100 to the immunochromatographic test strip 500 placed in the detection tank under the action of centrifugal force.

[0116] See Figure 17 In part (7), after the pre-centrifugation step, the mixture and plasma are brought into contact with the corresponding immunochromatographic test strips 500 and flow slowly on the immunochromatographic test strips 500. It should be noted that this step can be performed by starting a centrifuge at a very low speed, such as 200 rpm, so that the liquid is subjected to a small centrifugal force in the opposite direction, thereby slowing down the flow rate of the liquid on the immunochromatographic test strips 500, increasing the reaction time with the coated antibody on the test strip, and thus improving the reaction sensitivity. In other words, the centrifugation operation is for reaction sensitization. Of course, in some embodiments, this centrifugation operation may not be performed.

[0117] See Figures 1-17 Based on the protein detection chip provided in this application, this application also provides a protein detection method, including the following steps:

[0118] Step A: Take out the protein detection chip, add the blood sample, and then seal the weak area of ​​the first cover 201. The protein detection chip is pre-loaded with diluent (i.e., diluent reagent Q).

[0119] Step B: The protein detection chip is placed into the chip carrier 920 of the centrifuge with the end of the chip chip facing down near the mixing liquid release tank. The chip carrier 920 is hinged to the rotatable rotating frame 910 of the centrifuge, and the hinge axis J and the rotation axis Z of the rotating frame 910 are perpendicular to each other.

[0120] Step C: Start the centrifuge and use centrifugal force to control the transfer of blood sample and diluent within the protein detection chip until the detection cell becomes detectable. Stop the centrifuge, as the detection cell in the detectable state contains the optical signal to be detected.

[0121] It should be noted that "pre-filled with diluent" in the protein detection chip means that the diluent has been added to the protein detection chip before step A. Of course, in some embodiments, the protein detection chip taken in step A may not be pre-filled with diluent. In this case, step A may also include adding diluent reagent Q, that is, both diluent reagent Q and blood sample are added to the protein detection chip in step A. For example, if the protein detection chip does not have a reservoir 300, the first cover 201 may have a weak area corresponding to the diluent storage tank 110. In this way, in step A, the diluent reagent Q is added to the diluent storage tank 110 through the weak area on the first cover 201 corresponding to the diluent storage tank 110 using the same method as adding the blood sample. Then, the weak area of ​​the first cover 201 is sealed with, for example, a sealing film.

[0122] Depending on the structure of the protein detection chip, the diluent pre-loaded in the protein detection chip taken in step A may be stored directly in the diluent storage tank 110, or it may be stored in the storage box 300 and stored together with the storage box 300 in the diluent storage tank 110. If the protein detection chip is equipped with a storage box 300, the diluent can be added to the storage box 300 before the overall fabrication of the protein detection chip. In this case, the protein detection chip taken in step A already contains the diluent reagent Q. Step A may also include pulling the pull tab 301 on the storage box 300 to open the storage chamber of the storage box 300 from a closed state.

[0123] Depending on the centrifuge structure, in step B, the protein detection chip may be directly loaded into the chip carrier 920 of the centrifuge, or it may be loaded into the chip holder 930 and loaded into the centrifuge together with the chip holder 930. Depending on the protein detection chip structure, the fluorescence signal present in the detection cell that becomes detectable in step C may be formed on the immunochromatographic test strip 500 or on the micropillar array 810.

[0124] In step C, after the detection slot becomes detectable, there is an optical signal to be detected. The type of optical signal can be varied depending on the experimental method. For example, the optical signal can be a fluorescence signal, a chemiluminescence signal, a visual signal, or other different types. In some embodiments, the protein detection method may include step D after step C: after removing the protein detection chip from the centrifuge, inserting the protein detection chip with the end closest to the mixed liquid release tank into the chip slot of the detector 900, and detecting the fluorescence signal through the light-transmitting area of ​​the second cover plate 202 using the detection light path of the detector 900. Of course, in other embodiments, depending on the type of optical signal, the detection result information carried by the optical signal can be read in other ways after step C. For example, if the optical signal is a visual signal, the operator can directly observe the optical signal through the light-transmitting area of ​​the second cover plate 202 after removing the protein detection chip from the centrifuge, thereby obtaining the detection result.

[0125] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0126] The above description of the disclosed embodiments enables those skilled in the art to make or use this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A protein detection chip, comprising: The body is rectangular in shape and has a first face and a second face. The first face is provided with a diluent storage tank, a diluent release tank, a sample addition tank, a sample separation tank, a mixing tank and a mixed solution release tank arranged sequentially along the length of the body. The second face is provided with a detection tank extending along the length of the body. One end of the detection tank is connected to the mixed solution release tank through a transfer hole. The body is provided with a self-circulating ventilation structure. The self-circulating ventilation structure includes a first air passage provided on the first face, a second air passage provided on the second face, and a vent hole that connects the first face and the second face. The first air passage and the second air passage are connected through the vent hole. A first cover plate covers the first surface, and the first cover plate has a weak area corresponding to the sample dispensing slot; A second cover plate covers the second surface, and the second cover plate has a light-transmitting area corresponding to the detection groove.

2. The protein detection chip according to claim 1, characterized in that, The first surface is provided with at least one waste liquid tank, and the first air passage is connected to the dilution storage tank side of the waste liquid tank.

3. The protein detection chip according to claim 1, characterized in that, It includes a cross-shaped mixing channel disposed on the first surface, the mixing channel being located between the sample separation tank and the mixing tank.

4. The protein detection chip according to claim 3, characterized in that, include: A mixing column is located inside the mixing tank; Two mixing partitions are located inside the mixing tank and on the side of the mixing column near the mixing channel. The two mixing partitions are arranged in a figure-eight shape with their tips facing the mixing column. The ends of the two mixing partitions away from each other are connected to the inner wall of the mixing tank, and a gap is formed between the ends of the two mixing partitions close to each other.

5. The protein detection chip according to claim 1, characterized in that, It includes at least two sample dispensing slots, which are arranged along the width direction of the body and are located between the sample separation slot and the mixing slot.

6. The protein detection chip according to claim 5, characterized in that, It includes a first hydrophobic capillary channel extending in a serpentine shape disposed on the first surface, with the two ends of the first hydrophobic capillary channel respectively connected to the sample dispensing tank and the mixing tank.

7. The protein detection chip according to claim 1, characterized in that, The diluent storage tank is equipped with a liquid storage box, and the liquid storage box is provided with an easy-open strip for sealing the liquid outlet of the liquid storage chamber. The first end face of the main body away from the mixed liquid release tank is provided with an avoidance notch for the easy-open strip to extend out.

8. The protein detection chip according to any one of claims 1 to 7, characterized in that, The detection slot contains immunochromatographic test strips; or... The detection groove is equipped with a microcolumn array, and the surface of the microcolumns is coated with antibodies.

9. The protein detection chip according to claim 8, characterized in that, A positioning groove is formed on the second end face of the main body near the end of the mixture release tank. The positioning groove is located at the intersection of the second end face and the first face, or at the intersection of the second end face and the second face; and / or, The side of the main body away from the end of the mixture release tank is provided with a foolproof protrusion.

10. A protein detection method, comprising the following steps: Step A: Take the protein detection chip and add the blood sample. The protein detection chip is the protein detection chip as described in any one of claims 1 to 9, and the protein detection chip is pre-loaded with diluent. After adding the blood sample, seal the weak area of ​​the first cover corresponding to the sample loading slot. Step B: The protein detection chip is placed into the chip carrier of the centrifuge with the end closest to the mixture release tank facing down. The chip carrier is hinged to the rotatable rotating frame of the centrifuge, and the hinge axis is perpendicular to the rotation axis of the rotating frame. Step C: Start the centrifuge and use centrifugal force to control the transfer of blood sample and diluent within the protein detection chip until the detection cell becomes detectable. Stop the centrifuge, as the optical signal to be detected exists in the detectable cell.

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