Method for controlling, preventing and expelling insects through ecological biological liquid

Abstract

The invention relates to the technical field of agricultural ecology, in particular to a method for controlling, preventing and expelling insects through ecological biological liquid. Comprising the following steps: constructing an ecological planting base; insect repelling plants are planted; preparing a plant concentrated solution; preparing a fermented insect repelling liquid; compounding an ecological biological liquid; field application. According to the method for controlling, preventing and expelling insects through the ecological biological liquid, through the innovative design of closed planting base construction, hierarchical layout of insect expelling plants and the synergistic effect of the composite biological liquid, multi-dimensional synergistic prevention and control from field ecological blocking to biological liquid chemical expelling are achieved, and the effect of preventing and expelling insects through the ecological biological liquid is achieved while zero chemical residue is guaranteed. And the application defects of insufficient effective radius and short lasting period of the traditional ecological insect expelling technology are overcome.

Description

Technical Field

[0001] This invention relates to the field of agricultural ecological technology, and in particular to a method for controlling, preventing, and repelling insects using an ecological biological liquid. Background Technology

[0002] In agricultural production, pest control is a core element in ensuring crop yield and quality. While traditional chemical pesticides can quickly kill pests, long-term use can lead to problems such as excessive pesticide residues, damage to the soil ecosystem, increased pest resistance, and accidental damage to non-target organisms, seriously threatening food safety and ecological balance. To address these challenges, organic agriculture and green planting models urgently need to develop efficient, residue-free ecological pest control technologies.

[0003] Existing ecological pest control methods mainly include spraying plant-derived extracts, introducing natural enemies, and physical isolation, but they still have obvious limitations: (1) single plant extracts have a narrow target and short duration of action, making them difficult to deal with compound pests; (2) physical isolation facilities (such as insect nets) are expensive and difficult to block the migration of underground pests; (3) the active ingredients of bio-fermented insect repellents are unstable and are easily affected by environmental factors and become ineffective in the field. Especially for stubborn small pests such as aphids, thrips, and spider mites, existing ecological methods generally have technical bottlenecks such as small repellency radius, short control cycle, and complex compounding process. Summary of the Invention

[0004] This invention proposes an ecological biological liquid method for controlling, preventing, and repelling insects. Through the innovative design of constructing a closed planting base, hierarchical layout of insect-repelling plants, and synergistic effect of compound biological liquid, it achieves multi-dimensional synergistic control from field ecological barrier to chemical repellency of biological liquid. While ensuring zero chemical residue, it overcomes the application defects of traditional ecological insect repellent technology, such as insufficient effective radius and short duration of effect.

[0005] The technical solution adopted in this invention is: a method for controlling, preventing, and repelling insects using an ecological biological liquid, comprising the following steps: Step 1: Construct an ecological planting base: Select farmland with sufficient water supply, set up a closed fence, and divide the base into planting areas and organic fertilizer storage areas. The planting areas include independently set vegetable cultivation areas, pasture cultivation areas, and melon and fruit cultivation areas. Step 2, Planting insect-repelling plants: Cultivate bitter beans, bitter lettuce, and mint in the planting area; Step 3, prepare plant concentrate: collect the stems and leaves of bitter bean, bitter gourd and mint, purify them by physical pressing, soak the stems and leaves in warm water at 35±5℃ for 24-48 hours, and filter to obtain plant essence concentrate; Step 4, prepare fermented insect repellent liquid: crush bitter bean seeds into 60-80 mesh particles, mix with animal bile and animal oil in a weight ratio of 3:3:2.5, add 0.5% yeast of the total weight of the mixture, and ferment at 25-30℃ for 7-10 days. Step 5, compounding ecological biological liquid: mix the concentrated plant essence liquid from step 3 with the fermented insect repellent liquid from step 4 at a volume ratio of 1:1-1.5, bottle and store at room temperature. Step 6, Field application: Dilute the ecological biological liquid at a volume ratio of 1:50-100 and spray it on the crop leaves every 10 days, no more than 3 times per month.

[0006] As a further improvement of the present invention, the height of the enclosed fence in step one is not less than 1.8 meters, the material is metal mesh and biodegradable plastic, and the bottom of the fence is buried at a depth of ≥20cm to prevent underground pests from migrating.

[0007] As a further improvement of the present invention, in step one, each cultivated area is provided with an isolation belt with a width of ≥2m, and the isolation belt is planted with mint and rosemary as a secondary insect repellent barrier.

[0008] As a further improvement of the present invention, the warm water soaking in step three adopts a stepped temperature control process: the first stage is maintained at 40°C for 2 hours, the second stage is maintained at 35°C for 24 hours, and the third stage is maintained at 30°C until the end.

[0009] As a further improvement of the present invention, the animal bile in step four is selected from at least one of pig bile, ox bile and fish bile; the animal oil is refined lard with an acid value ≤2mgKOH / g.

[0010] As a further improvement of the present invention, in step four, the fermentation process is stirred twice a day for 10-15 minutes each time, and the oxygen concentration in the fermentation container is maintained at 8-12% vol.

[0011] As a further improvement of the present invention, a natural emulsifier is added during the compounding process in step five. The natural emulsifier is at least one of lecithin and gum arabic, and the amount added is 0.1-0.3% of the total weight of the mixture.

[0012] As a further improvement of the present invention, the spraying operation in step six is ​​carried out before sunrise and after sunset, the spraying amount is controlled at 30-50mL / m², and the leaf surface wetting rate is ≥80%.

[0013] As a further improvement of the present invention, the pests repelled include aphids, thrips, spider mites and horseflies. The ecological biological liquid is formed by the mixed fermentation of biological components extracted from plant and animal oils to form pest-averse substances, thereby achieving the repellent effect. This biological agent does not directly kill pests, but drives them away from crops. The effective repellency radius is ≥5m, and the effective period is 7-10 days.

[0014] The beneficial effects of this invention are as follows: By constructing a closed-loop ecological planting system and combining innovative compound biological agent technology, this invention forms a three-dimensional prevention and control network of physical barriers, biological repellency, and chemical-ecological synergy. This method breaks through the limitations of traditional ecological pest control and achieves broad-spectrum and long-lasting protection: multiple barriers significantly expand the pest repellency range and extend the effective action period; the stability of active ingredients is improved, and special processes enhance the ability to resist environmental interference, ensuring the reliability of field application; it takes into account both ecology and economy, using biodegradable materials and natural raw materials throughout the process to achieve zero chemical residues, while significantly reducing the overall cost through high dilution and low-frequency application, providing an efficient and green control path for stubborn pests. Attached Figure Description

[0015] Figure 1 This is a real-life image of bitter bean, illustrating the method for controlling, preventing, and repelling insects using an ecological biological liquid according to the present invention. Figure 2 This is a real-life photo of a pest control method based on the present invention, which involves using an ecological biological liquid to control, prevent, and repel insects. Detailed Implementation

[0016] To make the technical problems, technical solutions, and beneficial effects to be solved by this application clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the embodiments described herein are merely illustrative of this application and are not intended to limit this application.

[0017] This invention provides a method for controlling, preventing, and repelling insects using an ecological biological liquid, comprising the following steps: Step 1: Construct an ecological planting base: Select farmland with sufficient water supply, set up a closed fence, and divide the base into planting areas and organic fertilizer storage areas. The planting areas include independently set vegetable cultivation areas, pasture cultivation areas, and melon and fruit cultivation areas. Step 2, Planting insect-repelling plants: Cultivate bitter beans, bitter lettuce, and mint in the planting area; Step 3, prepare plant concentrate: collect the stems and leaves of bitter bean, bitter gourd and mint, purify them by physical pressing, soak the stems and leaves in warm water at 35±5℃ for 24-48 hours, and filter to obtain plant essence concentrate; Step 4, prepare fermented insect repellent liquid: crush bitter bean seeds into 60-80 mesh particles, mix with animal bile and animal oil in a weight ratio of 3:3:2.5, add 0.5% yeast of the total weight of the mixture, and ferment at 25-30℃ for 7-10 days. Step 5, compounding ecological biological liquid: mix the concentrated plant essence liquid from step 3 with the fermented insect repellent liquid from step 4 at a volume ratio of 1:1-1.5, bottle and store at room temperature. Step 6, Field application: Dilute the ecological biological liquid at a volume ratio of 1:50-100 and spray it on the crop leaves every 10 days, no more than 3 times per month.

[0018] In step one of this invention, the height of the enclosed fence is not less than 1.8 meters, and the material is metal mesh and biodegradable plastic. The bottom of the fence is buried at a depth of ≥20cm to prevent underground pests from migrating.

[0019] In step one of the present invention, each cultivated area is provided with an isolation belt with a width of ≥2m, and mint and rosemary are planted in the isolation belt as a secondary insect repellent barrier.

[0020] In step three of this invention, the warm water soaking adopts a stepped temperature control process: the first stage is maintained at 40°C for 2 hours, the second stage is maintained at 35°C for 24 hours, and the third stage is maintained at 30°C until the end.

[0021] In step four of this invention, the animal bile is selected from at least one of pig bile, ox bile, and fish bile; the animal oil is refined lard with an acid value ≤2mgKOH / g.

[0022] In step four of this invention, the fermentation process is stirred twice a day for 10-15 minutes each time, and the oxygen concentration in the fermentation container is maintained at 8-12% vol.

[0023] In step five of this invention, a natural emulsifier is added during compounding. The natural emulsifier is at least one of lecithin and gum arabic, and the amount added is 0.1-0.3% of the total weight of the mixture.

[0024] In step six of this invention, the spraying operation is carried out before sunrise and after sunset, with the spraying amount controlled at 30-50 mL / m², and the leaf surface wetting rate ≥80%.

[0025] The pests repelled by this invention include aphids, thrips, spider mites, and horseflies. The ecological biological liquid is formed by the mixed fermentation of biological components extracted from plant and animal oils to create pest-averse substances, thereby achieving the repellent effect. This biological agent does not directly kill pests, but repels them away from crops. The effective repellency radius is ≥5m, and the effective period is 7-10 days.

[0026] (I) Base Construction The site was selected on 10 mu of farmland in the North China Plain (groundwater level 1.5m), and a 2m high fence (metal mesh + polylactic acid biodegradable plastic composite material) was erected with a bottom burial depth of 25cm. Zoning: Vegetable area (3 mu, planted with cabbage), pasture area (2 mu, planted with alfalfa), and fruit and vegetable area (3 mu, planted with watermelon). Mint is planted in the intervals with a 2.5m wide isolation strip. Bitter beans, bitter herbs, and mint are planted along the inside of the fence at a density of 200 plants per acre.

[0027] (II) Preparation of biological fluid Plant concentrate: Sophora flavescens stems and leaves, Sophora japonica leaves, and mint leaves were mixed in a 1:1:1 ratio and purified by physical pressing. The stems and leaves were then soaked in 40℃ warm water for 2 hours, maintained at 35℃ for 24 hours, and then soaked at 30℃ for 10 hours to obtain a concentrate (yellow-green color, specific gravity 1.12 g / mL). Fermented insect repellent liquid: bitter bean seeds are crushed to 70 mesh, mixed with pig bile (acid value 1.8mgKOH / g) and refined lard (weight ratio 3:3:2.5), and 0.5% brewer's yeast is added; Ferment at 28℃ for 8 days (stirring twice a day for 12 minutes, O2 concentration 10% vol) to obtain a brownish-black viscous liquid.

[0028] (III) Field Application Processing group Dilution factor Spraying frequency Application rate Control group (conventional planting) Ecological biological liquid 1:80 10 days / time 40mL / m² Chemical pesticide (imidacloprid) Physical isolation zone No protection (iv) Insect repellent effect (monitoring of cabbage aphids) Group Repellency rate 3 days after drug application Shelf life Insect population density (heads / leaf) Effective radius Ecological biological liquid 92.5% 9 days 0.8±0.3 ≥5.2m Chemical pesticides 98.7% 15 days 0.3±0.1 Physical isolation zone 76.2% continued 2.1±0.5 Inside the barrier control group 35.6±8.7 Conclusion: The residual effect of the compound biological liquid is 60% longer than that of conventional pesticides, which is significantly better than that of physical isolation alone (p<0.01).

[0029] (I) Fermentation process optimization experiment Three sets of comparative methods were used for verification: Group A: The crushed bitter bean seeds were mixed with pig bile and refined lard in a ratio of 3:3:2.5 and then fermented in a stepped temperature-controlled manner (enzymatic hydrolysis at 40℃ for 2 hours → main fermentation at 35℃ for 24 hours → post-fermentation at 30℃ for 8 days). The mixture was stirred twice a day throughout the process and maintained at an oxygen concentration of 10% vol.

[0030] Group B: The crushed bitter bean seeds were mixed with pig bile and refined lard in a ratio of 3:3:2.5 and fermented at a constant temperature of 35℃ for 8 days, with stirring twice a day throughout the process and maintaining an oxygen concentration of 10% vol.

[0031] Group C: The crushed bitter bean seeds were mixed with pig bile and refined lard in a ratio of 3:3:2.5 and then fermented in a nitrogen-filled, sealed container at a constant temperature of 30℃ for 8 days.

[0032] The results showed that the matrine yield in group A reached 18.7 mg / g, which was 21.3% higher than that in group B (HPLC detection GB / T 20784-2022); the bile acid conversion rate was 87.5%, which was better than that in group B (71.6%). For spider mites, the repellency rate in group A reached 93.6% 72 hours after application, with a duration of action of 9 days (>80% effective threshold), which was 50% longer than that in group B. The anaerobic treatment in group C resulted in nearly half the activity loss, verifying the necessity of aerobic agitation.

[0033] (II) Synergistic Effect Experiment of Emulsifiers Comparing three schemes in a basic biological solution (plant concentrate: fermentation broth = 1:1.2): Group D: 0.2% lecithin added; Group E: 0.3% gum arabic added; Group F: no emulsifier added.

[0034] The results showed that the emulsion in group D did not separate after 72 hours of standing, and its leaf surface retention time was extended to 6.3 hours (compared to only 2.2 hours in group F). The contact angle decreased from 68.9° to 28.5°, confirming that lecithin can significantly enhance leaf spreadability. After simulating 20mm of rainfall, the repellency rate of group D against thrips remained at 88.7%, while that of group F plummeted to 42.6%. The gum arabic group (group E) performed better, but the cost increased by 30%. At 35℃, the effective period of group D reached 7 days, which was 75% longer than that of group F.

[0035] In summary, this invention provides an ecological biological liquid method for pest control, prevention, and repellency. Through a series of innovative measures, including constructing a closed ecological planting base, rationally arranging insect-repelling plants, optimizing the biological liquid preparation process, and adding natural emulsifiers, it achieves efficient, environmentally friendly, and sustainable pest control. This method not only effectively solves many problems associated with traditional chemical pesticides but also overcomes the limitations of existing ecological pest control technologies, providing a green, safe, and economically feasible pest control solution for agricultural production. In practical applications, this ecological biological liquid significantly improves pest repellency rates and duration of effectiveness, especially in dealing with stubborn small pests. Simultaneously, the implementation of this method helps protect the soil ecological environment, reduce pesticide residues, and ensure the quality and safety of agricultural products, meeting the development needs of organic agriculture and green planting models. With the deepening of the concept of sustainable agricultural development, this ecological biological liquid method for pest control, prevention, and repellency is expected to be more widely promoted and applied in the agricultural field, playing an important role in promoting the ecological transformation of agriculture.

[0036] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for controlling, preventing, and repelling insects using an ecological biological liquid, characterized in that, Includes the following steps: Step 1: Construct an ecological planting base: Select farmland with sufficient water supply, set up a closed fence, and divide the base into planting areas and organic fertilizer storage areas. The planting areas include independently set vegetable cultivation areas, pasture cultivation areas, and melon and fruit cultivation areas. Step 2, Planting insect-repelling plants: Cultivate bitter beans, bitter lettuce, and mint in the planting area; Step 3, prepare plant concentrate: collect the stems and leaves of bitter bean, bitter gourd and mint, purify them by physical pressing, soak the stems and leaves in warm water at 35±5℃ for 24-48 hours, and filter to obtain plant essence concentrate; Step 4, prepare fermented insect repellent liquid: Crush bitter bean seeds into 60-80 mesh particles, mix with animal bile and animal oil in a weight ratio of 3:3:2.5, add 0.5% yeast of the total weight of the mixture, and ferment at 25-30℃ for 7-10 days; Step 5, compounding ecological biological liquid: mix the concentrated plant essence liquid from step 3 with the fermented insect repellent liquid from step 4 at a volume ratio of 1:1-1.5, bottle and store at room temperature. Step 6, Field application: Dilute the ecological biological liquid at a volume ratio of 1:50-100 and spray it on the crop leaves every 10 days, no more than 3 times per month.

2. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, In step one, the height of the enclosed fence shall not be less than 1.8 meters, and the material shall be metal mesh and biodegradable plastic. The bottom of the fence shall be buried at a depth of ≥20cm to prevent underground pests from migrating.

3. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, In step one, each cultivated area is equipped with an isolation strip with a width of ≥2m, and mint and rosemary are planted in the isolation strip as a secondary insect-repelling barrier.

4. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, In step three, the warm water soaking adopts a stepped temperature control process: the first stage is 40℃ for 2 hours, the second stage is 35℃ for 24 hours, and the third stage is 30℃ until the end.

5. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, In step four, the animal bile is selected from at least one of pig bile, ox bile, and fish bile; the animal oil is refined lard with an acid value ≤2mgKOH / g.

6. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, In step four, the fermentation process is carried out by stirring twice a day for 10-15 minutes each time, and the oxygen concentration in the fermentation container is maintained at 8-12% vol.

7. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, In step five, a natural emulsifier is added during compounding. The natural emulsifier is at least one of lecithin and gum arabic, and the amount added is 0.1-0.3% of the total weight of the mixture.

8. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, The spraying operation in step six is ​​carried out before sunrise and after sunset, with the spraying amount controlled at 30-50 mL / m², and the leaf surface wetting rate ≥80%.

9. The method for controlling, preventing, and repelling insects using an ecological biological liquid according to claim 1, characterized in that, The pests repelled include aphids, thrips, spider mites, and horseflies. The ecological biological liquid is formed by the mixed fermentation of biological components extracted from plant and animal oils to create pest-averse substances, thus achieving the repellent effect. This biological agent does not directly kill pests, but repels them away from crops; the effective repellency radius is ≥5m, and the effective period is 7-10 days.

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