Anti-aging composition, firming and glowing essence containing same and preparation method of firming and glowing essence

Abstract

The invention relates to an anti-aging composition, a firming and glowing essence containing the anti-aging composition and a preparation method of the firming and glowing essence. The anti-aging composition is prepared from the following components in parts by weight: 1.5 to 6 parts of glyceryl polyether-26, 1.5 to 5 parts of nicotinamide, 0.25 to 1.2 parts of xylitol, 0.13 to 0.3 part of sodium hyaluronate, 0.01 to 0.3 part of carnosine, 0.01 to 0.1 part of PCA sodium, 0.005 to 0.05 part of hydrolyzed collagen, 0.006 to 0.03 part of palmitoyl tripeptide-5, 0.0008 to 0.01 part of hexapeptide-9 and 0.001 to 0.01 part of dipeptide diaminobutyryl benzyl amide diacetate. Test results show that the tightening and glowing essence developed by adopting the composition has the effects of instantly supplementing moisture for skin, promoting collagen generation, improving wrinkles and helping the skin to recover a supporting structure so as to slow down aging, and has a good market prospect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition, a firming and rejuvenating essence containing the composition, and a method for preparing the same. Background Technology

[0002] Currently, anti-aging products on the market mainly fall into four categories: water-based, microemulsion, cream, and pure oil. Water-based anti-aging products are popular with consumers due to their refreshing feel and transparent appearance. Regarding active ingredients, while mainstream active ingredients in the current anti-aging skincare field, such as retinol and Pro-Xylane, possess certain efficacy, they also have significant drawbacks. Retinol (such as retinoic acid and its derivatives), despite their clear anti-wrinkle and collagen-regenerating effects, suffers from poor water solubility and high photosensitivity, leading to insufficient formula stability and a reliance on oil encapsulation, which can easily cause skin irritation or peeling. Furthermore, its poor water solubility limits its application in lightweight water-based formulations, failing to meet consumers' demand for a refreshing feel. Pro-Xylane (hydroxypropyl tetrahydropyranotriol), as a glycosaminoglycan synthesis promoter, focuses its anti-aging mechanism on repairing the epidermal-dermal junction (DEJ), but high concentrations can easily lead to sticky and heavy formulations, limiting its applicability, especially in hot or humid environments, and affecting the user experience.

[0003] In the study of the mechanisms of water-based anti-aging products, existing anti-aging products mostly focus on single targets (such as collagen regeneration, anti-oxidation, or autophagy activation), while neglecting the core role of moisturizing as the foundation of anti-aging. The skin barrier function is the basis of skin life activities; however, current water-based formulations, designed to consider formulation costs and bundled with creams, often omit basic moisturizing ingredients, resulting in reduced weather resistance, poor stability of active ingredients, and low penetration efficiency. In conclusion, it is essential to develop an integrated solution that uses a moisturizing system as a base and synergistically incorporates anti-aging active ingredients to achieve a firming and rejuvenating serum with a dual-effect synergy of "barrier repair and deep anti-aging," thereby addressing the problems existing in current technologies and products. Summary of the Invention

[0004] Based on the above needs, this application provides an anti-aging composition, a firming and rejuvenating essence containing the composition, and a method for preparing the same. The anti-aging composition obtained through scientific and rational design in this invention can mimic extracellular matrix signals, possessing advantages such as precise targeting, low effective concentration, and high biocompatibility. It regulates the expression of genes involved in the synthesis of collagen, elastin, and hyaluronic acid. Simultaneously, this composition can increase the moisture content of the stratum corneum and enhance the epidermal barrier function. The firming and rejuvenating essence developed using this composition has immediate and long-lasting moisturizing effects, repairs the skin barrier, and improves facial wrinkles, thereby achieving the goal of anti-aging.

[0005] One object of the present invention is an anti-aging composition, characterized in that, by weight parts, it comprises 1.5-6 parts of glycerol polyether-26, 1.5-5 parts of nicotinamide, 0.25-1.2 parts of xylitol, 0.13-0.3 parts of sodium hyaluronate, 0.01-0.3 parts of carnosine, 0.01-0.1 parts of sodium PCA, 0.005-0.05 parts of hydrolyzed collagen, 0.006-0.03 parts of palmitoyl tripeptide-5, 0.0008-0.01 parts of hexapeptide-9, and 0.001-0.01 parts of dipeptide diaminobutyryl benzylamide diacetate.

[0006] Furthermore, the firming and rejuvenating essence containing the anti-aging composition is characterized in that the firming and rejuvenating essence is composed of an anti-aging composition, a solvent, a moisturizer, a pH adjuster, a skin conditioning agent, a thickener, and a thickening agent.

[0007] Furthermore, the firming and rejuvenating essence is characterized in that, by weight, it comprises 3.5-10 parts of an anti-aging composition, 88.7-92 parts of a solvent, 1.63-3.31 parts of a moisturizer, 0.18-0.25 parts of a pH adjuster, 0.54-3.31 parts of a skin conditioning agent, 0.188-0.271 parts of a thickener, and 0.3-0.5 parts of a preservative.

[0008] Furthermore, the firming and rejuvenating essence is characterized in that the solvent comprises 82-88 parts water, 1-3 parts butylene glycol, 5-7 parts glycerin, 0.3-0.9 parts 1,3-propanediol, 0.4-0.8 parts 1,2-hexanediol, 0.3-0.5 parts dipropylene glycol, 0.1-0.4 parts diglyceride, and 0.1-0.2 parts glyceryl caprylate. The humectant comprises 0.6-1.3 parts trehalose, 0.8-1.4 parts glyceryl polyether-26, 0.2-0.5 parts diethoxyethyl succinate, 0.02-0.08 parts sodium PCA, 0.01-0.08 parts sclerotium gum, 0.01-0.05 parts erythritol, 0.01-0.05 parts dehydrated xylitol, and 0.01-0.05 parts xylitol glucoside. The pH adjuster is 0.18-0.25 parts of tromethamine. The skin conditioning agent includes 0.3-0.6 parts of p-hydroxyacetophenone, 0.18-0.25 parts of carbomer, 0.05-0.15 parts of allantoin, 0.01-0.04 parts of carrageenan powder, and 0.01-0.03 parts of acetylated hyaluronic acid. The thickener comprises 0.005-0.015 parts xanthan gum, 0.18-0.25 parts carbomer, and 0.003-0.006 parts hydroxyethyl cellulose. The preservatives include 0.25-0.45 parts of phenoxyethanol, 0.004-0.01 parts of benzoic acid, and 0.003-0.008 parts of sorbic acid.

[0009] Furthermore, the method for preparing the anti-aging composition is characterized in that the preparation method includes: S1. Add glycerol polyether-26, xylitol and sodium hyaluronate to a mixing vessel, and stir at 30 rpm and 60°C to mix glycerol polyether-26, xylitol and sodium hyaluronate into a transparent and homogeneous mixture. S2. Set the vacuum degree of the mixing vessel to -0.06MPa and the stirring speed to 15 rpm to remove air bubbles from the mixture; S3. Cool the transparent homogeneous mixture to 30°C, and gradually add palmitoyl tripeptide-5, hexapeptide-9, dipeptide diaminobutyryl benzylamide diacetate, carnosine, sodium PCA and hydrolyzed collagen powder to mix and stir evenly to obtain an anti-aging composition.

[0010] Another object of the present invention is to provide the above-mentioned firming and rejuvenating essence containing the anti-aging composition and a method for preparing the same, wherein the preparation method includes the following steps: S1. Add the solvent and humectant to the emulsifying pot and stir until evenly mixed. Heat to 70-75℃ and stir until transparent and free of particles. Then cool to 60-65℃. S2. Apply a negative pressure of -0.06MPa to the emulsifying pot to remove air bubbles from the mixture. Add the skin conditioning agent and anti-aging composition to the emulsifying pot one by one, and stir at 25 rpm for 10 minutes until transparent and free of particles to obtain the intermediate product. S3. Apply a vacuum pressure of -0.06MPa to the emulsifying pot to remove air bubbles from the mixture, and cool it to 45-60℃. Add the thickener one by one to the emulsifying pot and stir at 25 rpm for 10 minutes until it is transparent and free of particles to obtain the intermediate product. S4. Add the pH adjuster and preservative ingredients to the emulsifying pot and stir at 25 rpm for 10 minutes to mix evenly, to obtain a firming and rejuvenating essence containing an anti-aging composition. The present invention has the following beneficial effects:

[0011] Niacinamide enhances skin radiance by inhibiting melanin transport; sodium hyaluronate, through the penetration effects of different molecular weights, improves the transdermal absorption rate and moisturizing longevity of hyaluronic acid, and together with glyceryl polyether-26 and sodium PCA, constructs a multi-layered water-locking network, providing hydration channels for the penetration of anti-aging active ingredients; xylitol combines small-molecule moisturizing and penetration-enhancing functions, optimizing the skin-friendliness of the formula, while strengthening the barrier function by regulating the skin's microbiome balance; signal peptide targeted delivery technology utilizes the "molecular chaperone" effect of xylitol derivatives to enhance the stability and targeted delivery efficiency of signal peptides, carnosine, and hydrolyzed collagen in a water-based environment, achieving synergistic effects between peptide activity and the moisturizing matrix. This anti-aging composition, through scientific and rational design and compounding research, and the solid construction of a system with a robust mechanism of action and synergistic effects, breaks away from the industry's chaotic practices of using active ingredients without scientific basis and in combination. In addition, this invention breaks through the limitations of the traditional anti-aging formula of "physical mixing" of active ingredients and moisturizers, and achieves the three-in-one synergistic effect of "moisturizing-repairing-anti-wrinkle" from the molecular design level, providing a new paradigm for the development of lightweight and efficient anti-aging products, and has good market prospects. Detailed Implementation

[0012] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.

[0013] Unless otherwise specified, the reagents, methods and equipment used in this invention are conventional reagents, methods and equipment in this technical field.

[0014] In the embodiments of this invention, "parts" refers to parts by mass. One of the anti-aging compositions is shown in Table 1 in parts by weight. Table 1. Components and proportions of the composition

[0015] Preparation method of the anti-aging composition S1. Add glycerol polyether-26, xylitol and sodium hyaluronate to a mixing vessel, and stir at 30 rpm and 60°C to mix glycerol polyether-26, xylitol and sodium hyaluronate into a transparent and homogeneous mixture. S2. Set the vacuum degree of the mixing vessel to -0.06MPa and the stirring speed to 15 rpm to remove air bubbles from the mixture; S3. Cool the transparent homogeneous mixture to 30°C, and gradually add palmitoyl tripeptide-5, hexapeptide-9, dipeptide diaminobutyryl benzylamide diacetate, carnosine, sodium PCA and hydrolyzed collagen powder to mix and stir evenly to obtain an anti-aging composition.

[0016] The components of the firming and rejuvenating serum containing the anti-aging composition are shown in Table 2 below. Table 2. Proportions of different components in the firming and rejuvenating serum Components Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Composition 1 5.0275 5.0275 4.5595 4.5595 3.0315 2.573 2.573 water margin margin margin margin margin margin margin Butylene glycol 2 1.6 2 2 1.6 1.6 2 glycerin 5.8 5 5.8 4.8 5 5 5.8 1,3-Propanediol 0.8 0.8 0.8 0.5 0.8 0.8 0.8 1,2-Hexanediol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 dipropylene glycol 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Diglycerides 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Glyceryl caprylate 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Trehalose 1.2 1 1.2 1.2 1 1 1.2 Erythritol 0.4 0.4 0.04 0.04 0.4 0.4 0.04 dehydrated xylitol 0.3 0.3 0.03 0.03 0.3 0.3 0.03 Xylitol glucoside 0.15 0.15 0.03 0.03 0.15 0.15 0.03 Diethoxyethyl succinate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Tromethamine 0.24 0.22 0.24 0.24 0.22 0.22 0.24 Sclerotium sclerotium gum 0.02 0.02 0.02 0.02 0.02 0.02 0.02 p-Hydroxyacetophenone 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Carbomer 0.24 0.23 0.24 0.24 0.23 0.23 0.24 Allantoin 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Acetylated hyaluronic acid 0.02 0.02 0.02 0.02 0.02 0.02 0.02 benzoic acid 0.006 0.006 0.006 0.006 0.006 0.006 0.006 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Sorbic acid 0.006 0.006 0.006 0.006 0.006 0.006 0.006 Wrinkled Cornflower Noodles 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Xanthan Gum 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Hydroxyethyl cellulose 0.005 0.005 0.005 0.005 0.005 0.005 0.005 Comparative Example 1 is an example in which composition 1 in Example 2 is replaced with composition 3. Comparative Example 2 is an example in which composition 1 in Example 2 is replaced with composition 4. Comparative Example 3 is an example in which composition 2 in Example 3 is replaced with composition 4.

[0017] Preparation method of firming and rejuvenating serum containing anti-aging composition The above examples and comparative examples were all prepared using the same preparation method, and the specific preparation steps are as follows: S1. Add the solvent and humectant to the emulsifying pot and stir until evenly mixed. Heat to 70-75℃ and stir until transparent and free of particles. Then cool to 60-65℃. S2. Apply a negative pressure of -0.06MPa to the emulsifying pot to remove air bubbles from the mixture. Add the skin conditioning agent and anti-aging composition to the emulsifying pot one by one, and stir at 25 rpm for 10 minutes until transparent and free of particles to obtain the intermediate product. S3. Apply a vacuum pressure of -0.06MPa to the emulsifying pot to remove air bubbles from the mixture, and cool it to 45-60℃. Add the thickener one by one to the emulsifying pot and stir at 25 rpm for 10 minutes until it is transparent and free of particles to obtain the intermediate product. S4. Add the pH adjuster and preservative ingredients to the emulsifying pot and stir at 25 rpm for 10 minutes to mix evenly, to obtain a firming and rejuvenating essence containing an anti-aging composition.

[0018] Stability and security testing Stability and safety tests were conducted on the samples from Examples 1-4 and Comparative Examples 1-3. The firming and rejuvenating essences prepared in Examples 1-4 and Comparative Examples 1-3 were placed under light at -10℃, 5℃, and 25℃, and under 25℃ and 40℃ for 90 days to observe their physicochemical properties and color; chicken embryo fluff tests were also performed. The tests showed that Examples 1-5 and Comparative Example 3 were stable in pH, viscosity, and centrifugal state, while Comparative Example 1-2 had low viscosity and separated into layers upon centrifugation, failing to meet product stability requirements. The toxicological test results for Examples 1-5 and Comparative Examples 1-3 showed safety and met cosmetic requirements. The test results are shown in Table 3. Table 3 Stability and safety test data Implementation / Comparative Example pH (direct measurement) Viscosity (63# 20r) Centrifuge (2000 rpm for 20 minutes) Toxicology Example 1 6.45 2100 No layering Safety Example 2 6.43 1970 No layering Safety Example 3 6.37 1850 No layering Safety Example 4 6.44 1830 No layering Safety Comparative Example 1 6.32 1890 No layering Safety Comparative Example 2 6.36 1800 No layering Safety Comparative Example 3 6.37 1790 No layering Safety

[0019] Sensory evaluation Skin sensitivity tests were performed on Examples 1-4 and Comparative Examples 1-3 to screen product groups suitable for market applications. By recruiting a skin feel testing team, training them, and ensuring they possess objective and accurate skin feel testing capabilities, blind tests were conducted on Examples 1-4 and Comparative Examples 1-3 using a scoring system (5 points, with higher scores indicating a better experience), and products that meet consumer user experience criteria were selected. The examples, especially Example 1, demonstrate that the smooth texture and moisturizing effect of the product are close to consumer usage habits. Long-term use shows that the wrinkle-reducing and firming effects meet consumers' anti-wrinkle and firming needs, indicating that the firming and rejuvenating essence containing the aforementioned composition provides a good user experience. Test results are shown in Table 4. Table 4 Sensory Evaluation Data Analysis Note: This is a 5-point rating system; a higher score indicates a better consumer experience.

[0020] Biochemical Anti-wrinkle and Firming Evaluation Test The anti-wrinkle and firming effects of the product were verified by elastase inhibition assay. The anti-wrinkle and firming ability of the samples was evaluated by comparing their inhibition rate against elastase with that of the positive control sample. Elastase was diluted to 0.1 U / mL with Tris-HCl buffer (0.1 mol / L, pH=8), N-succinyl-Ala-Ala-Ala-p-nitroaniline (AAAPAN) was dissolved and diluted to 1.0 mg / L, and epigallocatechin gallate (EGCG) was diluted to 80 mg / mL. Three parallel samples were prepared. Reagents were added according to the reaction system in Table 6 below, and the mixture was shaken well and reacted at room temperature for 30 min. The absorbance was measured at 410 nm. The average value of the parallel samples was taken as the final result. The sample was considered to have an anti-wrinkle and firming effect when the inhibition rate of the sample group was ≥ 30% of the inhibition rate of the positive control group. The test results are shown in Table 5. Table 5 Test conditions experimental group Sample solution (mL) AAAPAN (mL) EGCG solution (ml) Tris buffer (ml) Elastase (ml) Sample group 0.5 1.0 / / 0.5 Blank group / 1.0 / 1.0 / Positive control group / 1.0 0.5 / / negative control group / 1.0 / 0.5 0.5 Elastase inhibition formula: =1 - (OD sample group / positive control group - OD blank group) / (OD model control group - OD blank group) * 100% Table 6 Anti-aging data analysis

[0021] Cellular Anti-aging Evaluation Test The anti-wrinkle and firming effects of the product were verified by detecting the elastin content and type I collagen content of fibroblasts. Test principle: (1) Firming Effect: Sun exposure is a major cause of skin aging. After ultraviolet radiation irradiates the skin, it leads to the generation of a large number of free radicals, which further induces changes in ECM content. The main mechanisms include the following two aspects: First, it activates ECM degradation-related enzymes such as matrix metalloproteinases, thereby accelerating ECM degradation. Second, it inhibits ECM synthesis by inhibiting the TGFβ-Smad signaling pathway, thereby reducing ECM content. Elastin is the main protein that makes up elastic fibers. A decrease in its content leads to thinning of elastic fibers and reduced skin elasticity. Therefore, promoting an increase in elastin content can achieve a firming effect. (2) Anti-wrinkle effect: Collagen I is type I collagen. Collagen accounts for about 80% of the weight of the dermis. Collagen I assembles to form collagen fibers. Collagen fibers are multidirectional and gradually curl, which makes the dermis more three-dimensional and the skin fuller. Therefore, promoting the increase of Collagen I (type I collagen) content can resist the formation of wrinkles and improve wrinkles, thus having an anti-wrinkle effect. This experiment used fibroblasts as the detection tool and designed blank control (BC), negative control (NC), positive control (PC) and sample group respectively. Biological replicates were designed for each group. ELISA kits were used to detect changes in elastin content and type I collagen content to analyze the firming and anti-wrinkle effects of the firming and rejuvenating essence containing anti-aging composition. Firming Efficacy: Based on fibroblasts, compared with the blank control group, the elastin content in the negative control group was significantly decreased after UVA irradiation (P<0.01), indicating that UVA stimulation was effective in this test. Compared with the negative control group, the elastin content in the positive control group was significantly increased (P<0.01), meeting the quality control standards of this method, indicating that the experimental system was effective. Compared with the negative control group, the firming and rejuvenating essence containing the anti-aging composition showed a significant increase in elastin content at a concentration of 0.00016%. This indicates that the sample can increase elastin content at this concentration and has a firming effect. Anti-wrinkle efficacy: Based on fibroblasts, compared with the blank control group, the negative control group showed a significant decrease in type I collagen content after UVA irradiation (P<0.01), indicating that UVA stimulation was effective in this test. Compared with the negative control group, the positive control group showed a significant increase in type I collagen content (P<0.01), meeting the quality control standards of this method, indicating that the experimental system was effective. Compared with the negative control group, the firming and rejuvenating essence containing the anti-aging composition showed a significant increase in type I collagen content at a concentration of 0.00016%. This indicates that the sample can increase type I collagen content at this concentration and has anti-wrinkle efficacy. Prepare three parallel samples and add them to the reaction system according to Table 7 below. Table 7 Experimental Conditions experimental group UV radiation ascorbic acid(%) sample(%) Sample group 30min / 0.00016 Blank group / / / Positive control group 30min 0.0002 / negative control group 30min / / Formulas for the growth rate of elastin and type I collagen: = 1 - (OD sample group / positive control group - OD blank group) / (OD model control group - OD blank group) * 100% Table 8 Anti-aging Data Analysis The above embodiments illustrate specific implementations of the present invention. However, the above embodiments are only preferred embodiments of the present invention and do not limit any other implementations of the present invention. Any modifications, substitutions, or improvements made in any other way based on the technical principles of the present invention are included within the protection scope of the present invention.

Claims

1. An anti-aging composition, characterized in that, By weight, it includes 1.5-6 parts of glycerol polyether-26, 1.5-5 parts of nicotinamide, 0.25-1.2 parts of xylitol, 0.13-0.3 parts of sodium hyaluronate, 0.01-0.3 parts of carnosine, 0.01-0.1 parts of sodium PCA, 0.005-0.05 parts of hydrolyzed collagen, 0.006-0.03 parts of palmitoyl tripeptide-5, 0.0008-0.01 parts of hexapeptide-9, and 0.001-0.01 parts of dipeptide diaminobutyryl benzylamide diacetate.

2. The use of the anti-aging composition according to claim 1 in cosmetics with anti-aging effects.

3. The firming and rejuvenating essence containing the anti-aging composition according to claim 2, characterized in that, The firming and rejuvenating essence is composed of the anti-aging composition according to claim 1, a solvent, a moisturizer, a pH adjuster, a skin conditioning agent, a thickener, and a preservative.

4. The solvent according to claim 3, characterized in that, The solvent includes one or more of water, butanediol, glycerol, 1,3-propanediol, 1,2-hexanediol, dipropylene glycol, diglycerol, and glyceryl caprylate.

5. The humectant according to claim 3, characterized in that, The humectant includes one or more of trehalose, diethoxyethyl succinate, sclerotium gum, erythritol, dehydrated xylitol, and xylitol glucoside.

6. The pH adjuster according to claim 3, characterized in that, The pH adjuster is tromethamine.

7. The skin conditioning agent according to claim 3, characterized in that, The skin conditioning agent includes one or more of p-hydroxyacetophenone, allantoin, carrageenan, and acetylated hyaluronic acid.

8. The thickener according to claim 3, characterized in that, The thickener includes one or more of xanthan gum, carbomer, and hydroxyethyl cellulose.

9. The preservative according to claim 3, characterized in that, The preservatives include one or more of phenoxyethanol, benzoic acid, and sorbic acid.

10. The method for preparing the anti-aging composition according to claim 1, characterized in that, The preparation method includes: S1. Add glycerol polyether-26, xylitol and sodium hyaluronate to a mixing vessel, and stir at 30 rpm and 60°C to mix glycerol polyether-26, xylitol and sodium hyaluronate into a transparent and homogeneous mixture. S2. Set the vacuum degree of the mixing vessel to -0.06MPa and the stirring speed to 15 rpm to remove air bubbles from the mixture; S3. Cool the transparent homogeneous mixture to 30°C, and gradually add palmitoyl tripeptide-5, hexapeptide-9, dipeptide diaminobutyryl benzylamide diacetate, carnosine, sodium PCA and hydrolyzed collagen powder to mix and stir evenly to obtain an anti-aging composition.

11. The method for preparing the firming and rejuvenating essence containing the composition according to claims 3 and 10, characterized in that, The preparation method includes: S1. Add the solvent and humectant to the emulsifying pot and stir until evenly mixed. Heat to 70-75℃ and stir until transparent and free of particles. Then cool to 60-65℃. S2. Apply a negative pressure of -0.06MPa to the emulsifying pot to remove air bubbles from the mixture. Add the skin conditioning agent and anti-aging composition to the emulsifying pot one by one, and stir at 25 rpm for 10 minutes until transparent and free of particles to obtain the intermediate product. S3. Apply a vacuum pressure of -0.06MPa to the emulsifying pot to remove air bubbles from the mixture, and cool it to 45-60℃. Add the thickener one by one to the emulsifying pot and stir at 25 rpm for 10 minutes until it is transparent and free of particles to obtain the intermediate product. S4. Add the pH adjuster and preservative ingredients to the emulsifying pot and stir at 25 rpm for 10 minutes to mix evenly, to obtain a firming and rejuvenating essence containing an anti-aging composition.

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