A stem cell composition for improving aging and metabolic dullness and a preparation method thereof

Abstract

The present application relates to the technical field of skin care products, and in particular to a stem cell composition for improving aging and metabolic dullness and a preparation method thereof.The composition is compounded by an animal umbilical cord extract, a schizosaccharomyces pombe fermentation product lysate, DNA sodium, a tripeptide-105 and a cold orchid extract, and the five components synergistically regulate, accelerate melanin metabolism, reduce melanin deposition, inhibit melanin production, stimulate cell regeneration, replace old and worn-out cells, repair damaged cells, enhance cell vitality, inhibit inflammatory response, calm the cell microenvironment, and achieve the best long-acting lightening, whitening, barrier repair, soothing irritation, and improvement of severe acne clinical effects in terms of efficacy.The composition not only has faster and longer-lasting effects, but also has good safety and applicability, and has a broad market application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care products, in particular to a stem cell composition for improving aging and metabolic dullness and a preparation method thereof. BACKGROUND

[0002] In existing whitening products, whitening ingredients mainly reduce melanin production by inhibiting tyrosinase activity (such as hydroquinone, kojic acid, arbutin, etc.), but the production of melanin is closely related to ultraviolet radiation, endocrine, inflammatory reaction and other factors. A single mechanism of inhibiting melanin production often cannot comprehensively solve problems such as color spots and uneven skin tone; many whitening ingredients with good whitening effect, such as vitamin C and certain phenolic compounds, have poor stability and are easily affected by air, light and temperature; some whitening ingredients have poor skin penetration, which may be difficult to penetrate the skin barrier or cannot effectively reach the deep layer of the skin, thereby limiting the whitening effect; the effect of some whitening ingredients is not long-lasting, and the skin tone returns to normal after stopping use, or during use, due to external factors (such as ultraviolet light), a rebound effect occurs.

[0003] Consumer interest in natural ingredients continues to rise, especially plant extracts and antioxidant ingredients such as green tea extract, licorice extract, and cold orchid extract, which have become mainstream ingredients in whitening products. Natural ingredients are favored by more and more sensitive skin consumers due to their fewer side effects. Whitening products on the market gradually tend to combine multiple effective ingredients to achieve comprehensive whitening effects. For example, many products contain both melanin production inhibiting ingredients (such as arbutin and kojic acid) and antioxidant ingredients (such as vitamin C and niacinamide).

[0004] In summary, these problems reflect the urgent need to research a skin care composition that can regulate the skin's pigment metabolism mechanism from the root, improve aging and metabolic dullness, red blood vessels, and moderate to severe acne, repair the barrier, and restore the skin to be water, tender, and bright with long-lasting effects, thereby meeting the market's urgent demand for high-efficiency, safe, and long-lasting whitening products. SUMMARY

[0005] The purpose of the present application is to overcome the deficiencies of the prior art and provide a stem cell composition for improving aging and metabolic dullness and a preparation method thereof, which regulates the skin's pigment metabolism mechanism from the root, improves aging and metabolic dullness, red blood vessels, and moderate to severe acne, repairs the barrier, and restores the skin to be water, tender, and bright with long-lasting effects.

[0006] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0007] In a first aspect, the present application provides a stem cell composition for improving aging and metabolic dullness, comprising the following components: animal umbilical cord extract, Bifidobacterium fermentation product lysate, DNA sodium, tripeptide-105 and cold orchid extract, and the weight ratio of the animal umbilical cord extract, Bifidobacterium fermentation product lysate, DNA sodium, tripeptide-105 and cold orchid extract is (0.1-1):(0.1-1):(1-10):(0.5-1):(5-15);

[0008] The preparation method of the cold orchid extract comprises the following steps: taking dried cold orchid, crushing and sieving, adding 50-90% ethanol according to the solid-liquid ratio of 1g:20-50mL, mixing uniformly, and then ultrasonic extraction under the condition of 250-350W for 80-100min to obtain the extraction liquid and residue; the residue is repeatedly extracted 2-4 times according to the solid-liquid ratio of 1g:20-50mL; and the extraction liquids are combined and concentrated, and then freeze-dried to obtain the cold orchid extract.

[0009] The animal umbilical cord extract is mainly derived from the umbilical cord of mammals, especially the umbilical cord of animals such as cows and pigs. The umbilical cord is a key physiological structure in the gestation process of mammals, providing a material exchange channel between the mother and the fetus. The umbilical cord extract contains rich nutrients such as amino acids, proteins, vitamins, minerals and growth hormones, which can promote the metabolism and repair process of skin cells. By regulating the skin microenvironment, the function of the skin barrier is enhanced, thereby improving the skin's resistance to external stimuli such as ultraviolet rays and pollutants. A strong skin barrier can reduce the skin's sensitivity to the external environment, thereby reducing pigmentation or uneven skin tone caused by external factors.

[0010] Bifidobacterium is a kind of probiotic bacteria, which widely exists in the human intestinal tract and has the effect of maintaining intestinal health. Bifidobacterium fermentation product is a component produced by fermentation of Bifidobacterium under laboratory conditions. This product contains various enzymes, nucleic acids, amino acids, antioxidants and other substances. Through further processing such as lysis, active cell components can be extracted, which are believed to be able to directly act on the skin. Bifidobacterium fermentation product lysate can reduce melanin production by reducing the synthesis and activity of tyrosinase, relieve skin pigmentation, and thus whiten the skin.

[0011] DNA sodium contains rich nucleic acid components, which can directly promote the repair and regeneration of skin cells. By enhancing the metabolic function of skin cells, DNA sodium can promote the repair of damaged skin cells and help the skin barrier restore normal function. The repair and regeneration of skin cells play an important role in improving uneven skin tone and reducing melanin deposition. The nucleic acid components contained in DNA sodium can play an antioxidant role, scavenge free radicals and slow down the oxidation reaction. The removal of free radicals not only prevents skin aging, but also reduces the synthesis of melanin caused by oxidation, thereby effectively reducing pigmentation and melanin deposition.

[0012] Tripeptide-105 is a small molecule peptide composed of three amino acids (amino acids are the basic building blocks of proteins), commonly used as an active ingredient in skincare products. It can effectively penetrate the skin barrier and directly act on skin cells. This makes it easier for the skin to absorb than large molecular proteins or complexes, providing more precise skincare effects.

[0013] Cold orchid extract is derived from cold orchid (a species of orchid) and is widely used in traditional Chinese medicine. Cold orchid extract has gradually become an important ingredient in the field of skin care due to its rich bioactive components such as flavonoids, polysaccharides, and phenolic acids. Cold orchid extract helps remove aged cells in the stratum corneum and reduces the deposition of melanin on the skin surface, making the skin tone brighter and more uniform.

[0014] Preferably, the weight ratio of the animal umbilical cord extract, the Schizosaccharomyces pombe fermentation product lysate, DNA sodium, tripeptide-105 and cold orchid extract is (0.1-0.8): (0.1-0.8): (1-5): (0.5-1): (5-10).

[0015] Preferably, the weight ratio of the animal umbilical cord extract, the Schizosaccharomyces pombe fermentation product lysate, DNA sodium, tripeptide-105 and cold orchid extract is (0.1-0.5): (0.1-0.3): (1-3): (0.5-0.6): (5-8).

[0016] In a second aspect, the present application provides the use of the stem cell composition for improving aging and metabolic dullness in the preparation of skin care products according to the first aspect.

[0017] Preferably, the skin care product is any one of toner, emulsion, cream, mask, serum or spray, and the added amount of the composition is 0.5%-3% of the total weight of the skin care product.

[0018] In a third aspect, the present application provides a serum, comprising the following raw materials by weight percentage: 0.5%-3% of the composition of the first aspect, 0.05%-0.5% of a thickening agent, 3%-20% of a humectant, 0.2%-5% of a preservative, and 0.05%-0.1% of a pH regulator, and the balance being deionized water.

[0019] Preferably, the thickening agent comprises at least one of xanthan gum, Carbopol 940, AVC, and high molecular cellulose.

[0020] Preferably, the humectant comprises at least one of glycerol, butylene glycol, and low molecular sodium hyaluronate.

[0021] Preferably, the preservative comprises at least one of 1,2-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0022] Preferably, the pH regulator comprises at least one of arginine, tromethamine, and disodium EDTA.

[0023] In a fourth aspect, the present application provides a preparation method of the serum of the third aspect, comprising the following steps:

[0024] S1, mixing the humectant, the thickening agent, the pH regulator, and the deionized water, heating to 75-85℃, and then homogenizing at a speed of 1100-1300 rpm for 3-7 min, and then keeping warm after homogenization, to obtain a pre-prepared A phase;

[0025] S2, mixing the preservative, heating to 80-90℃ to melt, to obtain a pre-prepared B phase;

[0026] S3, after cooling the pre-prepared A phase to 55-65℃, stirring and mixing the pre-prepared B phase at a speed of 250-350 rpm, then cooling to below 40-50℃, adding the stem cell composition of any one of claims 1-3 and the essence, and continuing to stir for 3-7 min, finally adding the pH regulator to adjust the pH to 6.0-6.5, and then stopping stirring, discharging, to obtain the serum.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] This invention provides a stem cell composition for improving age-related and metabolic-related dullness. It effectively combines animal umbilical cord extract, bifida ferment lysate, sodium DNase, tripeptide-105, and Cymbidium goeringii extract to synergistically accelerate melanin metabolism, reduce melanin deposition, and inhibit melanin production. Simultaneously, it stimulates cell regeneration, metabolizes old and damaged cells, repairs damaged cells, enhances cell vitality, inhibits inflammatory responses, and calms the cellular microenvironment. Efficacy-wise, it achieves optimal long-lasting brightening, whitening, barrier repair, soothing, and improvement of severe acne clinical effects. It also empowers the cellular level, providing the active factors and nutrients needed for cell growth and improving cellular metabolism. This invention reduces the rapid metabolism of melanin in the epidermis and minimizes latent pigmentation in the dermis. By activating dormant stem cells, it induces cell self-repair and regeneration, efficiently replacing damaged cells, replenishing young cells, and restoring skin radiance. Simultaneously, it inhibits inflammation in the epidermis and restores a healthy cellular microenvironment. It fundamentally regulates the skin's pigment metabolism mechanism, improving age-related dullness, metabolic dullness, rosacea, and moderate to severe acne, repairing the skin barrier, and restoring skin's hydration and radiance with lasting effects. Furthermore, through long-term tracking of subjects' skin conditions, this invention can also improve symptoms of moderate to severe acne (such as rosacea), repair the skin barrier, improve rosacea, and even out skin tone. Attached Figure Description

[0029] Group The scratch area graphs for the positive control group, comparative example 1 group, and example 1 group in test case 1 at 0h, 6h, and 24h, respectively;

[0030] Animal umbilical cord extract This is a comparison image of brown area pigmentation in the subjects of the application group 1 at days 0 and 56, used in test example 3;

[0031] Schizosaccharomyces ferment lysate This is a comparison image of brown area pigmentation in the subjects of the group in Example 1 at days 0 and 56, as used in Test Example 3;

[0032] DNA sodium The image shows a comparison of redness levels in the subjects of the comparison application group 1 at days 0 and 56 in test example 3.

[0033] Tripeptide-105 The redness comparison charts of the subjects in the group of Example 1 at day 0 and day 56 are used in Test Example 3;

[0034] Yunnan bletilla extract The images show the acne condition of the subjects in the comparison application group 1 at days 0 and 56 in test case 3.

[0035] Yunnan bletilla extract number The images show the acne comparison of the subjects in the group of subjects in Example 1 at days 0 and 56, respectively, in Test Example 3. Detailed Implementation

[0036] For better illustrating the purposes, technical solutions and advantages of the present application, the present application will be further described in combination with specific examples.

[0037] The raw materials used in the following examples and comparative examples are as follows:

[0038] The animal umbilical cord extract was purchased from Shaanxi Nine States Stem Cell Science and Technology Industrial Park;

[0039] The Schizosaccharomyces pombe fermentation product lysate was purchased from Beijing Belilas Biotechnology Co., Ltd.;

[0040] DNA sodium was purchased from Shanghai Haoyun Trade Co., Ltd.;

[0041] Tripeptide-105 was purchased from Weiqi Technology Co., Ltd.;

[0042] Cymbidium kanran was purchased from Shao Hua Shijing Garden;

[0043] Collagen was purchased from Shanghai Maier Chemical Technology Co., Ltd.;

[0044] Bacillus fermentation product was purchased from Yancheng Biological Technology (Guangzhou) Co., Ltd.;

[0045] DNA calcium was purchased from Shanghai Haoyun Trade Co., Ltd.;

[0046] Cymbidium kanran extract was purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.;

[0047] Tripeptide-6 was purchased from Chengdu Jinglin Biotechnology Co., Ltd.

[0048] Other materials, reagents, etc. used in the examples can be obtained from commercial channels if there is no special description.

[0049] The preparation method of Cymbidium kanran extract ① specifically includes the following steps: dry Cymbidium kanran is taken, crushed, and passed through a 30-mesh sieve, 70% ethanol is added at a solid-liquid ratio of 1g:30mL and mixed uniformly, and then ultrasonic extraction is performed at 300W for 90min, and the extract and residue are obtained by filtration; the residue is repeatedly extracted 3 times with 70% ethanol at a solid-liquid ratio of 1g:30mL; the extract is concentrated, freeze-dried, and treated to obtain the Cymbidium kanran extract.

[0050] The preparation method of Cymbidium kanran extract ② specifically includes the following steps: dry Cymbidium kanran is taken, crushed, and passed through a 30-mesh sieve, 50% ethanol is added at a solid-liquid ratio of 1g:50mL and mixed uniformly, and then ultrasonic extraction is performed at 250W for 100min, and the extract and residue are obtained by filtration; the residue is repeatedly extracted 2 times with 50% ethanol at a solid-liquid ratio of 1g:50mL; the extract is concentrated, freeze-dried, and treated to obtain the Cymbidium kanran extract.

[0051] The preparation method of the Cymbidium kanran extract ③ specifically comprises the following steps: taking dried Cymbidium kanran, crushing, and passing through a 30-mesh sieve, then adding 90% ethanol according to a material-liquid ratio of 1 g:20 mL, uniformly mixing, and ultrasonically extracting for 80 min under the condition of 350 W, and filtering to obtain an extract and filter residue; the filter residue is repeatedly extracted four times according to a material-liquid ratio of 1 g:20 mL by adding 90% ethanol; and the extract is concentrated, and freeze-dried to obtain the Cymbidium kanran extract.

[0052] The component composition (weight parts) of the stem cell composition of examples 1-7 in the application is shown in the following table 1.

[0053] Table 1 Component and weight parts of the stem cell composition of examples 1-7

[0054] Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Group 0.3 0.2 2 0.55 7 ① 24 h cell migration rate (%) 0.3 0.2 2 0.55 7 ② blank control group 0.3 0.2 2 0.55 7 ③ positive control group 0.1 0.1 1 0.5 5 ① Example 1 0.5 0.3 3 0.6 8 ① Example 2 0.8 0.8 5 1 10 ① Example 3 1 1 10 1 15 ①

[0055] Comparative example 1

[0056] The difference between comparative example 1 and example 1 is that no animal umbilical cord extract, bifida yeast fermentation product lysate and tripeptide-105 are added in comparative example 1, and the missing amount is supplemented by DNA sodium and Cymbidium kanran extract in a weight ratio of 2:7.

[0057] Comparative example 2

[0058] The difference between comparative example 2 and example 1 is that no animal umbilical cord extract is added in comparative example 2, and the missing amount is supplemented by bifida yeast fermentation product lysate and tripeptide-105 in a weight ratio of 0.2:0.55.

[0059] Comparative example 3

[0060] The difference between comparative example 3 and example 1 is that no bifida yeast fermentation product lysate is added in comparative example 3, and the missing amount is supplemented by animal umbilical cord extract and tripeptide-105 in a weight ratio of 0.3:0.55.

[0061] Comparative example 4

[0062] The difference between comparative example 4 and example 1 is that no tripeptide-105 is added in comparative example 4, and the missing amount is supplemented by animal umbilical cord extract and bifida yeast fermentation product lysate in a weight ratio of 0.3:0.2.

[0063] Comparative example 5

[0064] The difference between comparative example 5 and example 1 is that no animal umbilical cord extract is added in comparative example 5, and an equal amount of collagen is used instead.

[0065] Comparative example 6

[0066] Comparative Example 6 differs from Example 1 in that the Schizosaccharomyces pombe fermentation product lysate is not added, and an equal amount of Bacillus fermentation product is used instead.

[0067] Comparative Example 7

[0068] Comparative Example 7 differs from Example 1 in that the DNA sodium is not added, and an equal amount of DNA calcium is used instead.

[0069] Comparative Example 8

[0070] Comparative Example 8 differs from Example 1 in that the tripeptide-105 is not added, and an equal amount of tripeptide-6 is used instead.

[0071] Comparative Example 9

[0072] Comparative Example 9 differs from Example 1 in that the Cymbidium kanran extract is not added, and an equal amount of Phalaenopsis aphrodite extract is used instead.

[0073] Comparative Example 10

[0074] Comparative Example 10 differs from Example 1 in that the animal umbilical cord extract, the Schizosaccharomyces pombe fermentation product lysate, the DNA sodium, the tripeptide-105, and the Cymbidium kanran extract are used in a weight ratio of 0.55:0.2:2:0.3:7.

[0075] Comparative Example 11

[0076] Comparative Example 11 differs from Example 1 in that the animal umbilical cord extract, the Schizosaccharomyces pombe fermentation product lysate, the DNA sodium, the tripeptide-105, and the Cymbidium kanran extract are used in a weight ratio of 0.3:0.55:2:0.2:7.

[0077] Test Example 1: Test of the ability of the composition to repair skin cells

[0078] A single layer of adherent cells cultured in an in vitro culture dish or plate is scored in the central region of cell growth with a gun tip or other hard object, the cells in the central portion are removed, and the cells are then cultured for a set time, and the ability of the cells to gradually enter the blank area from the edge of the scratch to heal the "scratch" is judged based on the ability of the cells to grow and migrate; this can simulate the wound healing process, and is used to evaluate the migration ability of cells at the wound site.

[0079] Test substance: The compositions prepared in Examples 1-7 and Comparative Examples 1-11 are each weighed out according to the formulation amount, mixed, and diluted 10-fold with distilled water to obtain a test sample solution.

[0080] Experimental materials: Cell line: HaCaT (human immortalized keratinocytes), P17, purchased from Beina Biotechnology; fetal bovine serum; Gibco DMEM basal medium; phosphate-buffered saline (PBS); penicillin (100 U / mL), streptomycin (100 µg / mL); 0.25% trypsin; cell culture flasks; multi-porous cell culture plates; 0.22 µm microporous filter membrane; wound healing 2-well insert (ibidi, REF:80209).

[0081] Experimental procedure: Take out a 12-well plate, and use sterile forceps to fix the 2-well wound healing inserts in the middle of the corresponding wells of the plate. Gently press the healing inserts to fix them in place. Inoculate the logarithmic growth phase cells at 5.0 × 10⁻⁶ cells / well. 4 70 µL / well were seeded into 2-well wound healing inserts, with 3 replicates per group. Cells were returned to the incubator and cultured for 24 h until confluence. The culture plate was removed, and the 2-well wound healing inserts were removed to create "wounds." Cells were washed with PBS to remove detached cells. Cells in the blank control group were cultured in 200 µL of PBS solution, the positive control group in 200 µL of DMEM basal medium containing 0.5% fetal bovine serum, and the test group in 200 µL of DMEM basal medium containing 0.1% of the test substance. Cell migration was recorded at 0 h, 6 h, and 24 h at fixed locations. Data was processed using the following method:

[0082] Scratch area = scratch gap width × length;

[0083] Cell migration rate = (0 h scratch area - 24 h scratch area) / 0 h scratch area × 100%;

[0084] Table 2. Data from the Skin Cell Repair Capacity Test

[0085] Example 4 Example 5 Example 6 15.83 Example 7 85.23 Comparative Example 1 99.86 Comparative Example 2 98.43 Comparative Example 3 98.28 Comparative Example 4 97.61 Comparative Example 5 96.45 Comparative Example 6 95.24 Comparative Example 7 93.87 Comparative Example 8 50.83 Comparative Example 9 58.27 Comparative Example 10 56.32 Comparative Example 11 57.54 Group 75.74 IL-1β concentration (pg / mL) 64.62 blank control group 69.17 model group 72.39 positive control group 80.66 Example 1 86.18 Example 2 87.25

[0086] Test Example 2: Inflammation Suppression Test of the Composition

[0087] Test substance: Weigh the compositions prepared in Examples 1-7 and Comparative Examples 1-11 according to the formula amount, mix them well and dilute them 10 times with distilled water to obtain the test sample solution.

[0088] Experimental materials: The cell line used was mouse macrophage RAW264.7, purchased from the Cell Resource Center of the Institute of Basic Medicine, Chinese Academy of Medical Sciences, fetal bovine serum (FBS, Gibco), DMEM high-sugar medium (DMEM, Gibco), dimethyl sulfoxide (DMSO, Sigma), phosphate buffer (PBS, Gibco), thiazolyl blue (MTT, Sigma), mouse interleukin-1β enzyme-linked immunosorbent assay kit (Mouse IL-1β ELISA kit, Boster).

[0089] The groups were set as shown in Table 3, and the specific experimental steps were as follows:

[0090] (1) Inoculation: inoculate the cells into a 24-well plate at a density of 2.0 x 10 4 cells / well, add 100 μL of fetal bovine serum to each well, and incubate at 37°C in a 5% CO2 incubator for 24 h; discard the original culture medium and wash with PBS buffer 3 times;

[0091] (2) Grouping: the blank control group and the model group were added with 100 μL of DMEM medium, the sample group was added with 100 μL of DMEM medium containing 0.3% concentration of the sample (compositions prepared in Examples 1-7 and Comparative Examples 1-11, respectively), and the positive control group was added with 100 μL of DMEM medium containing 0.001% concentration of dexamethasone, and continued to be cultured at 37°C in a 5% CO2 incubator for 24 h; 3 replicate wells were set for each group;

[0092] (3) LPS induction: discard the supernatant and wash with PBS buffer 3 times, add DMEM medium to the blank control group, add DMEM medium containing 0.1 μg / mL concentration of LPS to the model group, the sample group and the positive control group, and continue to be cultured at 37°C in a 5% CO2 incubator for 24 h, then collect the supernatant;

[0093] (4) Inflammatory factor content detection: take the cell supernatant of each well, and detect the cell inflammatory factor content according to the ELISA kit operation instruction;

[0094] (5) Data processing: the data obtained in the experiment were processed and plotted by Excel software; statistical analysis was performed by SPSS17.0, all measurement data were expressed as mean ± standard deviation; one-way ANOVA was used for comparison between groups, and when P <0.05, the difference was considered to be significant, and the results are shown in Table 3.

[0095] Table 3 Inflammation inhibition data

[0096] Example 3 Example 4 Example 5 2.89±0.19 Example 6 50.28 ± 5.73 # ]] Example 7 16.10 ± 1.52 #* ]] Comparative Example 1 16.04 ± 1.81 #* ]] Comparative Example 2 18.63 ± 2.47 #*▲ ]] Comparative Example 3 17.94 ± 1.85 #*▲ ]] Comparative Example 4 19.72 ± 1.69 #*▲ ]] Comparative Example 5 19.83 ± 2.04 #*▲ ]] Comparative Example 6 20.88 ± 1.93 #*▲ ]] Comparative Example 7 21.14 ± 2.15 #*▲ ]] Comparative Example 8 37.32 ± 3.96 #*▲ ]] Comparative Example 9 33.75 ± 4.86 #*▲ ]] Comparative Example 10 35.17 ± 4.52 #*▲ ]] Comparative Example 11 34.23 ± 4.41 #*▲ ]] Figure 1 25.15 ± 2.84 #*▲ ]] Figures 2-7 29.60 ± 3.32 #*▲ ]] ​ 27.97 ± 3.18 #*▲ ]] ​ 26.02 ± 3.40 #*▲ ]] ​ 24.34 ± 2.75 #*▲ ]] ​ 23.83 ± 2.51 #*▲ ]] ​ 22.16 ± 2.47 #*▲ ]]

[0097] Note: Compared with the blank control group: P < 0.05 is indicated by "". # "*" indicates a negative result compared to the model group; "*" indicates a negative result compared to the positive control group; "*" indicates a negative result compared to the positive control group. ▲ "express.

[0098] From the data of Example 1 and Comparative Examples 1-9 in Tables 2 and 3 and ​ It can be seen that the composition of Example 1, which is composed of five components including animal umbilical cord extract, Bifida ferment lysate, sodium DNA, tripeptide-105, and Cymbidium goeringii extract, showed a significant increase in cell migration rate and a significant decrease in IL-1β concentration over 24 hours. This indicates that the five components have a synergistic effect, and the composition prepared by them has repair and anti-inflammatory effects, thus having an excellent effect on improving age-related and metabolic dullness.

[0099] Based on the data from Examples 1, 4-7, and Comparative Examples 10-11 in Tables 2 and 3, it can be seen that when the weight ratio of animal umbilical cord extract, Bifida ferment lysate, sodium DNA, tripeptide-105, and Cymbidium goeringii extract is (0.1-0.5):(0.1-0.3):(1-3):(0.5-0.6):(5-8), the composition has the best effect on improving age-related and metabolic-related dullness.

[0100] Application Example 1-7 and Comparative Application Example 1-12

[0101] The compositions of Examples 1-7 and Comparative Examples 1-11 were added to the serum at a concentration of 3 wt% to obtain the serums of Application Examples 1-7 and Comparative Application Examples 1-11. The formulations are shown in Table 4.

[0102] The preparation methods of the serums used in Application Examples 1-7 and Comparative Application Examples 1-11 include the following steps:

[0103] S1. Mix the humectant, thickener, pH adjuster and deionized water, heat to 80°C, and homogenize at 1200 rpm for 5 min. After homogenization, keep warm for later use to obtain the pre-prepared phase A.

[0104] S2. Mix the preservatives and heat to 85°C to melt them, to obtain the pre-prepared phase B.

[0105] S3. After cooling the pre-prepared phase A to 60°C, add the pre-prepared phase B at 300 rpm and stir to mix. Then cool to below 45°C, add the stem cell composition and fragrance according to any one of claims 1-3 and continue stirring for 5 min. Finally, add a pH adjuster to adjust the pH to 6.0, then stop stirring, discharge the material, and obtain the essence.

[0106] Table 4. Serum formulations of application examples 1-7 and comparative application examples 1-11

[0107]

[0108] Comparative application example 12 (blank control group)

[0109] Comparative application example 12 differs from application example 1 in that the serum of comparative application example 12 does not add the composition, and an equal amount of deionized water is used instead of the composition, and the preparation method is the same as application example 1.

[0110] Test example 3: clinical whitening, repair, soothing, and acne-removing efficacy test of serum

[0111] This test example verifies the efficacy of the serums of application examples 1-7 and comparative application examples 1-12 on facial whitening, repair, soothing, and acne removal;

[0112] Test sample: serums prepared from application examples 1-7 and comparative application examples 1-12;

[0113] Experimental method: According to the “Cosmetic Safety Technical Specifications” (2015) test, 95 volunteers aged 18-45 with dry and sensitive skin (lactic acid stinging score > 3, skin moisture content 20 C.U~50 C.U) were randomly divided into 19 groups, 5 people in each group. Under normal circumstances, the volunteers used the product on the whole face, taking 1g of serum and applying it to the face, and then massaging and absorbing it. The volunteers were in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10%, and the improvement of the facial indicators was quantified and photographed using instruments. The test time was the indicator before use and 56 days after use. Skin-Glossymeter GL 200 was used to test skin brightness, Tewameter TM Hex was used to test trans-epidermal water loss (TEWL), Skin-Colorimeter CL400 and Visia 7 (IPP) were used to test skin redness and brown pigment, and a dermatologist assessed the severity of acne. The improvement rate was calculated according to the following formula:

[0114] Improvement rate before and after use = |X 使用前均值 −X 使用后均值 | / X 使用前均值 × 100%;

[0115] The calculated data is shown in Table 5 below;

[0116] Table 5. Test data of serums of application examples 1-7 and comparative application examples 1-12

[0117]

[0118] From the data of application example 1, comparative application examples 1-9 in Table 5 and ​ It can be seen that the TEWL, brightness, redness, brown area pigment and acne improvement rate of the composition of example 1 prepared by compounding animal umbilical cord extract, Schizosaccharomyces ferment lysate, DNA sodium, tripeptide-105 and cold orchid extract are all significantly increased, indicating that the five components of animal umbilical cord extract, Schizosaccharomyces ferment lysate, DNA sodium, tripeptide-105 and cold orchid extract have synergistic effect, and the prepared serum has repair and anti-inflammatory effects, thereby having excellent effects of improving aging type and metabolic type dullness.

[0119] From the data of application example 1, application examples 4-7 and comparative application examples 10-11 in Table 5, it can be seen that when the weight ratio of animal umbilical cord extract, Schizosaccharomyces ferment lysate, DNA sodium, tripeptide-105 and cold orchid extract is (0.1-0.5):(0.1-0.3):(1-3):(0.5-0.6):(5-8), the prepared serum has the best effect of improving aging type and metabolic type dullness.

[0120] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A stem cell composition for improving age-related and metabolic-related dullness, characterized in that, It comprises the following components: animal umbilical cord extract, Bifida ferment lysate, sodium DNate, tripeptide-105, and Cymbidium goeringii extract, wherein the weight ratio of the animal umbilical cord extract, Bifida ferment lysate, sodium DNate, tripeptide-105, and Cymbidium goeringii extract is (0.1-0.5):(0.1-0.3):(1-3):(0.5-0.6):(5-8); The preparation method of the Cymbidium goeringii extract specifically includes the following steps: take dried Cymbidium goeringii, crush and sieve it, add 50-90% ethanol at a material-to-liquid ratio of 1g:20-50mL and mix evenly, then ultrasonically extract at 250-350W for 80-100min, filter to obtain extract and residue; add 50-90% ethanol to the residue at a material-to-liquid ratio of 1g:20-50mL and repeat extraction 2-4 times; combine the extracts and concentrate and freeze-dry to obtain the Cymbidium goeringii extract.

2. The use of the stem cell composition for improving age-related and metabolic dullness as described in claim 1 in the preparation of skin care products.

3. The application of the stem cell composition for improving aging-related and metabolic dullness as described in claim 2 in the preparation of skincare products, characterized in that, The skincare product is any one of toner, lotion, cream, mask, serum or spray, and the amount of the composition added is 0.5%-3% of the total weight of the skincare product.

4. An essence, characterized in that, The raw materials include the following weight percentages: 0.5%-3% of the composition as described in claim 1, 0.05%-0.5% of thickener, 3%-20% of humectant, 0.2%-5% of preservative and 0.05%-0.1% of pH adjuster, with the balance being deionized water.

5. The essence as described in claim 4, characterized in that, The raw material is selected from at least one of (a)-(d): (a) The thickener comprises at least one of xanthan gum, carbomer 940, AVC and high molecular weight cellulose; (b) The moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; (c) The preservative includes at least one of 1,2-propanediol, 1,2-hexanediol and p-hydroxyacetophenone; (d) The pH adjuster includes at least one of arginine and tromethamine.

6. The method for preparing the essence according to claim 5, characterized in that, Includes the following steps: S1. Mix the humectant, thickener, pH adjuster and deionized water, heat to 75-85℃, and homogenize at 1100-1300 rpm for 3-7 minutes. After homogenization, keep warm for later use to obtain the pre-prepared phase A. S2. Mix the preservatives and heat to 80-90℃ to melt them, to obtain the pre-prepared phase B. S3. Cool the pre-prepared phase A to 55-65℃, add the pre-prepared phase B at 250-350 rpm and stir to mix. Cool to 40-50℃, add the stem cell composition and fragrance as described in claim 1 and continue stirring for 3-7 minutes. Finally, add a pH adjuster to adjust the pH to 6.0-6.5, then stop stirring, discharge the material, and obtain the essence.

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