Traditional Chinese medicine composition for treating qi deficiency and collateral arthralgia type depression

Abstract

The invention discloses a traditional Chinese medicine composition for treating qi deficiency and collateral arthralgia type depression. The composition is mainly prepared from the following traditional Chinese medicines in parts by weight: 25 to 400 parts of ginseng, 30 to 500 parts of cassia twig, 30 to 600 parts of radix paeoniae alba, 30 to 150 parts of radix curcumae and 30 to 150 parts of honey-fried licorice root. The composition can also comprise 20-200 parts of fresh ginger. Tests prove that the treatment effect of the composition on qi-deficiency collateral-arthralgia type depression is equivalent to or better than that of fluoxetine, the composition has remarkable treatment effects of tonifying qi and dredging collaterals while resisting depression, the symptoms of weakness, wind intolerance, gastrointestinal weakness and blood microcirculation injury of patients are effectively relieved, and the positive drug fluoxetine does not have the effects.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically relating to a traditional Chinese medicine composition for treating depression of the Qi deficiency and blood stasis type. Background Technology

[0002] Depression is a common mental illness, mainly characterized by persistent low mood, insomnia, and loss of appetite, severely impacting patients' quality of life. According to the World Health Organization, there are over 300 million people suffering from depression globally, and its incidence is increasing annually. The pathogenesis of depression is not fully understood, but research indicates that multiple factors, including genetics, environment, psychology, and biology, work together to cause it. Current treatments for depression include medication, psychotherapy, and physical therapy, with medication being the primary and widely used approach. However, existing clinical drugs have significant side effects and poor efficacy, leading more and more patients to explore alternative treatments using traditional Chinese medicine. In traditional Chinese medicine, depression is referred to as "Yu Zheng," "Bai He Bing," and "Zang Zao," among other names. Its occurrence and development are closely related to the body's condition, with liver dysfunction, spleen dysfunction, heart malnourishment, and imbalances in the yin, yang, qi, and blood of the internal organs being its main pathogenesis. Unlike most commonly used antidepressants that target the nervous system, Traditional Chinese Medicine (TCM) believes that different TCM formulas should be used to treat patients with depression based on their specific causes and mechanisms, achieving holistic regulation at multiple levels and targeting multiple points. This approach offers high safety and addresses both the symptoms and the root cause. Studies have shown that TCM formulas have therapeutic effects on depression by regulating neurotransmitters, improving cerebral blood circulation, modulating immune function, and managing psychological stress. These formulas not only alleviate symptoms but also improve quality of life, providing new ideas and methods for the prevention and treatment of depression. Therefore, seeking new TCM formulas for treating depression is urgently needed. Summary of the Invention

[0003] This invention provides a traditional Chinese medicine composition for treating depression of the Qi deficiency and blood stasis type. The composition, by weight, mainly consists of the following traditional Chinese medicines:

[0004] Ginseng 25-400 parts, cinnamon twig 30-500 parts, white peony root 30-600 parts, turmeric 30-150 parts, prepared licorice root 30-150 parts.

[0005] This composition may also include 20-200 parts of ginger.

[0006] The preferred proportions of this composition are: 90 parts ginseng, 90 parts cinnamon twig, 150 parts white peony root, 100 parts turmeric, and 120 parts prepared licorice root.

[0007] Another preferred ratio of this composition is: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 20-100 parts fresh ginger.

[0008] In this composition, ginseng can be replaced by one or two of the following: astragalus, five-finger peach, codonopsis, and ginseng; cinnamon twig can be replaced by polygala; white peony can be replaced by one or two of angelica and he shou wu; turmeric can be replaced by one or two of fritillaria and citron; and prepared licorice can be replaced by one or two of jujube and wheat kernel.

[0009] Preparation method of freeze-dried Chinese medicine powder: Wash all medicinal materials except ginseng, mix them in the above proportions, add 8-10 times the total weight of the medicinal materials and soak for 30 minutes. Then, decoct each time to 400 ml, and combine the two decoctions. For ginseng, decoct separately according to the above method and proportions. Finally, combine all the decoctions, filter, and concentrate to obtain a concentrated extract. Then, freeze-dry the extract to obtain freeze-dried powder.

[0010] Using conventional pharmaceutical manufacturing processes, the above raw materials can be prepared into commonly used clinical drug dosage forms, such as pills, tablets, granules, oral liquids, capsules, ointments, emulsions, chewable tablets, and injections.

[0011] This formula uses ginseng as the chief ingredient to replenish vital energy and address the root cause; cinnamon twig and white peony root are the assistant ingredients to replenish qi and warm yang, nourish yin and harmonize the blood, and warm and unblock the meridians, thus enhancing the qi-replenishing and meridian-unblocking effects of ginseng; turmeric is added to clear heat and resolve phlegm, open the chest and relieve depression, and promote the smooth flow of the meridians; prepared licorice root is used as the guiding ingredient to tonify the spleen and stomach, harmonize the effects of all the herbs, and assist their overall efficacy. The entire formula is meticulously formulated, working together to replenish vital energy, promote the flow of yang qi, invigorate blood and unblock the meridians, and soothe the mind and spirit; it has a rapid onset of action, significant therapeutic effects, no adverse reactions, and high safety. Experiments have shown that this herbal composition is comparable to or better than fluoxetine in treating depression of the qi deficiency and meridian obstruction type, and while antidepressant, it also has significant qi-replenishing and meridian-unblocking effects, effectively relieving symptoms such as fatigue, aversion to wind, gastrointestinal weakness, and impaired blood microcirculation; while the positive-active drug fluoxetine does not have these effects. This herbal composition has a simple formulation, readily available raw materials, strong indication specificity, and a simple preparation process, making it suitable for industrial production. Attached Figure Description

[0012] Figure 1 This is a diagram illustrating the effects of different doses of this composition on depressive-like behavior in a CUMS-induced depressed mouse model;

[0013] Subfigure A shows the weight change in CUMS-induced depression model mice during drug administration; subfigure B shows the standardized statistical results of sucrose preference in CUMS-induced depression model mice; subfigure C shows the standardized statistical results of the percentage of immobility time during the forced swimming test (FST) in CUMS-induced depression model mice (CON: blank control; CUMS: chronic mild unpredictable stress; Fluoxetine: fluoxetine, a positive drug for depression; SPT: sucrose preference test; FST: forced swimming test). Experimental results are expressed as mean ± standard error (Mean ± SEM). One-way ANOVA was used to test the significance of the data; * indicates p < 0.05, ** indicates p < 0.01.

[0014] Figure 2 This is a diagram illustrating the therapeutic effect of this composition on the Qi deficiency-like phenotype in CUMS model animals;

[0015] Subgraph A shows the statistics of mouse gripping force; subgraph B shows the statistics of mouse movement time in the rotarod experiment; subgraph C shows the statistics of mouse food intake during the CUMS experiment period; subgraph D shows the statistics of mouse water intake during the CUMS experiment period; subgraph E shows the statistics of mouse fecal water content during the CUMS experiment period; subgraph F shows the statistics of the number of times mice crossed grids in the CUMS wind-avoidance experiment; and subgraph G shows the statistics of the time mice spent in each area during the CUMS wind-avoidance experiment.

[0016] Figure 3 This image illustrates the therapeutic effect of this composition on the cerebral paralysis-like phenotype in CUMS model animals; Sub-image A shows representative laser speckle images of cerebral perfusion in each group; Sub-image B shows representative laser speckle images of blood flow perfusion in the right hind limb in each group; Sub-image C shows the statistical results of cerebral perfusion in each group; Sub-image D shows the statistical results of perfusion in the right hind paw in each group; Sub-image E shows the statistical results of reactive hyperemia time in the right hind paw in each group (PU: perfusion unit).

[0017] Figure 4 The figures illustrate the effects of the compositions in Examples 1 to 9 on reducing the sugar water preference rate in CUMS model animals. The serial numbers of the examples correspond to the formulation numbers in the figures.

[0018] Figure 5 The figures illustrate the effect of the compositions of Examples 10 to 18 on reducing the sugar water preference rate in CUMS model animals. The serial numbers of the examples correspond to the formulation numbers in the figures.

[0019] Figure 6 These are illustrations showing the effect of the compositions of Examples 19 to 27 on reducing the sugar water preference rate in CUMS model animals. The serial numbers of the examples correspond to the formulation numbers in the figures.

[0020] Figure 7 The figures show the effects of the compositions of Examples 28 to 31 on reducing the sugar water preference rate in CUMS model animals. The serial numbers of the examples correspond to the formulation numbers in the figures. Detailed Implementation

[0021] The present invention will be further described below in conjunction with specific embodiments.

[0022] I. Verification of the efficacy of the combination of the present invention and determination of the optimal dosage.

[0023] 1. Experimental method

[0024] 1.1 Establishment and grouping of experimental animal models

[0025] 120 SPF-grade C57BL / 6JNifdc mice, male, 7 weeks old, were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. The animal license number is: SCXK(Beijing)2021-0006. The feeding environment temperature was 25±2°C, the humidity was 55±5%, and the light was alternated between light and darkness for 12 hours. During the experiment, the animals were allowed to eat and drink water freely. After one week of adaptive feeding of the animals, an open field test was conducted on all animals to exclude animals with abnormal mental states or impaired motor abilities, so as to ensure the progress of subsequent experiments.

[0026] Chronic unpredictable mild stress (CUMS) model: C57BL / 6JNifdc mice were selected and a stress depression model animal was established using CUMS. The model group mice were exposed to the following stressors. The specific stress process plan is shown in Table 1.

[0027] Table 1 CUMS modeling stimulation schedule

[0028]

[0029] 1.2 Drug preparation

[0030] The original drug of the preparation of the present invention is a yellowish-brown traditional Chinese medicine freeze-dried powder. The specific ratio is 90 parts of ginseng, 90 parts of cinnamon twig, 150 parts of white peony root, 100 parts of turmeric, and 120 parts of roasted licorice root. The freeze-dried powder of the preparation of the present invention was dissolved with pure water to prepare high, medium and low doses. Fluoxetine was dissolved with pure water to prepare a 20 mg / ml liquid medicine as the positive drug. The new compound was administered by gavage at a dose of 10 ml / kg, that is, the doses were 22.8 g crude drug / kg (high dose), 11.4 g crude drug / kg (medium dose) and 5.7 g crude drug / kg (low dose), while fluoxetine was administered as the positive drug at a dose of 20 mg / kg, and the blank group was given the same volume of pure water.

[0031] 1.3 Drug treatment

[0032] After the experimental modeling was completed, the model mice were randomly divided into 5 groups of 12 mice each: CUMS model group, CUMS + low-dose new formulation group, CUMS + medium-dose new formulation group, CUMS + high-dose new formulation group, and CUMS + fluoxetine group. The blank control group and CUMS model group were administered pure water by gavage once daily; the CUMS + low-dose, medium-dose, and high-dose new formulation groups were administered a lyophilized aqueous solution of the new formulation by gavage once daily; and the CUMS + fluoxetine group was administered fluoxetine aqueous solution by gavage once daily. Behavioral experiments were conducted 14 days after drug administration.

[0033] 1.4 Evaluation of Depressive-like Phenotype (see Table 2 for details)

[0034] Table 2 Evaluation indicators of depressive behavior in mice

[0035]

[0036] 1.5 Statistical Methods

[0037] Statistical analysis was performed using SPSS 19.0 software. Experimental results are expressed as mean ± standard error (Mean ± SEM). One-way ANOVA was used to test the significance of the data. * indicates p < 0.05, and ** indicates p < 0.01. The final results were imported into Prism 9.3 for plotting.

[0038] 1.6 Experimental Results

[0039] Depressive-like behaviors in CUMS mice were assessed using body weight, sucrose test, and forced swimming test.

[0040] Mouse weight changes:

[0041] Fourteen days after administration, the CUMS group mice showed a significant decrease in body weight compared to the blank control group. However, after drug treatment, mice in the low, medium, and high doses of the new compound and the positive control group (fluoxetine) showed an increase in body weight, demonstrating that the new compound can improve the weight loss in CUMS mice, and its efficacy is comparable to that of the positive control group (see...). Figure 1 A).

[0042] Sugar water preference experiment

[0043] The results of saccharide preference analysis showed that, compared with the blank control group, the saccharide preference rate of mice in the CUMS model group was significantly reduced; while the saccharide preference rate of mice in the low, medium and high dose groups of the new compound was significantly increased. This indicates that the anhedonia phenotype in CUMS model mice was improved after administration of the new compound, and that this therapeutic effect was comparable to that of fluoxetine (see [link to study].) Figure 1 B).

[0044] Forced swimming experiment

[0045] Forced swimming test results showed that, compared with the blank control group, the immobility time of CUMS model mice was significantly reduced; the immobility time of mice in the high-dose group of the new compound was significantly reduced, while the low-dose group had no effect on improving the immobility time of model mice. This indicates that the medium and high doses of the new compound can have the same effect as fluoxetine in improving the despair behavior of CUMS model mice, and the therapeutic effect is comparable to that of fluoxetine (see...). Figure 1 C).

[0046] Based on the above, low, medium, and high doses of the new compound significantly improved the weight loss and sucrose preference in CUMS mice; however, the low dose did not significantly improve the despair state in CUMS mice. Secondly, considering the difficulty of gavage administration of high-dose new compound, this study subsequently selected the medium dose of the new compound as the experimental study dose.

[0047] II. Detection of Qi-deficiency-like behavior in mice using the combination of the present invention

[0048] 1. Determination of indicators for detecting Qi deficiency in mice

[0049] By retrieving 3766 clinical articles related to depression and 2960 clinical articles related to Qi deficiency from before 2024, inclusion and exclusion criteria were established for these articles. Articles that did not meet the inclusion criteria were removed. Data extraction and standardization were then performed on the collected articles. Standardized data resulted in a database of depressive symptoms / signs and a database of Qi deficiency symptoms / signs. Based on similar symptom_ids in both databases, common symptoms were summarized, resulting in 300 common symptoms of depression and Qi deficiency. Furthermore, based on the characteristics of small animal testing indicators, the following indicators were ultimately established as the detection indicators for "Qi deficiency" in mice (see Table 3).

[0050] Table 3 Indicators for detecting Qi deficiency in mice

[0051]

[0052] 2. Experimental Results

[0053] Here, we use the rotarod test and grip test to assess the "fatigue" state of mice, fecal water content to assess the gastrointestinal function of mice, and the wind avoidance test to assess the "wind aversion" of mice.

[0054] 2.1 Evaluation of "fatigue" symptoms in CUMS mice

[0055] Compared with normal control mice, the CUMS group mice had a significantly reduced rotator dwell time; however, after administration of the new compound, the rotator dwell time of the mice was significantly increased (see...). Figure 2A). Compared with the blank control group, the grip strength of mice in the CUMS group was significantly reduced; after treatment with the new compound, the grip strength of mice was significantly increased (see...). Figure 2 B). Both the rotarod test and the grip test demonstrated that CUMS mice exhibited a "weak" phenotype, and this phenomenon could be improved after treatment with the new compound, but fluoxetine administration had no effect on improving the above indicators.

[0056] 2.2 Evaluation of gastrointestinal function in CUMS mice

[0057] Mice in the CUMS group exhibited loose, easily broken fecal particles. Compared to the blank control group, the fecal water content of the CUMS group mice was increased. Administration of the new compound significantly reduced the fecal water content of the model mice, but fluoxetine administration did not show any improvement. There were no significant differences in food and water intake among the mice (see...). Figure 2 CE).

[0058] 2.3 Evaluation of "wind aversion" symptoms in CUMS mice

[0059] The wind avoidance test can be used to detect the clinical indicator of "wind aversion." Compared with the windless area, mice in all groups spent less time in both low-wind-speed and high-wind-speed areas. Compared with the blank control group, mice in the CUMS group spent more time in the windless area and significantly less time in the high-wind-speed area, with fewer crossings. After administration of the new compound, CUMS mice spent more time in the high-wind-speed area and crossed more areas, while fluoxetine administration did not show any improvement in the above symptoms (see...). Figure 2 FG).

[0060] III. Detection of paralysis-like behavior in mice using the combination of the present invention

[0061] 1. Determination of Indicators for Detecting Collateral Circulation in Mice

[0062] By retrieving 3766 clinical articles related to depression and 3341 clinical articles related to blood stasis / collateral arthralgia from before 2024, inclusion and exclusion criteria were established for these articles. Articles that did not meet the inclusion criteria were removed. Data extraction and standardization were then performed on the collected articles. Standardized data resulted in a database of depressive symptoms / signs and a database of qi deficiency symptoms / signs. Based on similar symptom_ids in these two databases, common symptoms were summarized, resulting in 156 common symptoms of depression and collateral arthralgia. Furthermore, based on the characteristics of small animal detection indicators, the following indicators were ultimately established as detection indicators for "collateral arthralgia" in mice (see Table 4).

[0063] Table 4. Detection indicators of "Luo Bi" in mice

[0064]

[0065]

[0066] Spearman correlation analysis was performed on the above symptom assessment indicators within a variable range to explore the potential association between them. The results showed that in the CUMS model, microvascular assessment variables (meningeal microvascular perfusion, right hind paw microvascular perfusion, and reactive hyperemia time) were significantly correlated with depressive behaviors (sucrose preference and forced swimming immobility time). Therefore, we selected meningeal microvascular perfusion, right hind paw microvascular perfusion, and reactive hyperemia time as evaluation indicators for "collateral arthritis".

[0067] 2. Experimental Results

[0068] This study evaluated the therapeutic effect of the new compound on microcirculatory dysfunction in CUMS model mice using laser Doppler experiments (see...). Figure 3 (AB). The results showed that, compared with the blank control group, the CUMS model group mice had significantly reduced cerebral and paw blood perfusion and prolonged reactive hyperemia time; after treatment with the new compound, the cerebral and paw blood perfusion of mice significantly increased and the reactive hyperemia time was shortened (see AB). Figure 3 (CE). This study demonstrated that the microcirculatory damage in the whole body and brain of CUMS model mice was restored after administration of the new compound, but mice treated with fluoxetine did not show significant changes in cerebral blood flow perfusion, paw blood flow perfusion, or reactive hyperemia.

[0069] IV. Verification of the antidepressant effect of this composition with different ratios

[0070] Example 1 (Formula 1): 25 parts ginseng, 500 parts cinnamon twig, 500 parts white peony root, 150 parts turmeric, and 150 parts prepared licorice root.

[0071] The drug preparation and testing methods are the same as above.

[0072] The sucrose preference test has become the most commonly used behavioral detection method for depression in the CUMS model due to its direct association with core depressive symptoms, ease of operation, high sensitivity, and well-defined neural mechanisms. Therefore, the efficacy of this traditional Chinese medicine composition was evaluated using the sucrose preference test.

[0073] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0074] Example 2: 400 parts ginseng, 30 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, and 150 parts prepared licorice root.

[0075] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0076] Example 3: 400 parts ginseng, 500 parts cinnamon twig, 30 parts white peony root, 150 parts turmeric, and 150 parts prepared licorice root.

[0077] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0078] Example 4: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 30 parts turmeric, and 150 parts prepared licorice root.

[0079] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0080] Example 5: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, and 30 parts prepared licorice root.

[0081] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0082] Example 6: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, and 150 parts prepared licorice root.

[0083] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0084] Example 7: 25 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, and 150 parts prepared licorice root.

[0085] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0086] Example 8: 400 parts ginseng, 30 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 200 parts fresh ginger.

[0087] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0088] Example 9: 400 parts ginseng, 30 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 150 parts fresh ginger.

[0089] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 4 .

[0090] Example 10: 400 parts ginseng, 500 parts cinnamon twig, 30 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 200 parts fresh ginger.

[0091] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0092] Example 11: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 30 parts turmeric, 150 parts prepared licorice root, and 200 parts fresh ginger.

[0093] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0094] Example 12: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 30 parts prepared licorice root, and 200 parts fresh ginger.

[0095] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0096] Example 13: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 100 parts fresh ginger.

[0097] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0098] Example 14: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 50 parts fresh ginger.

[0099] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0100] Example 15: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 20 parts fresh ginger.

[0101] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0102] Example 16: 60 parts ginseng, 180 parts cinnamon twig, 200 parts white peony root, 100 parts turmeric, and 150 parts prepared licorice root.

[0103] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0104] Example 17: 150 parts ginseng, 80 parts cinnamon twig, 200 parts white peony root, 100 parts turmeric, 100 parts prepared licorice root, and 150 parts fresh ginger.

[0105] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0106] Example 18: 150 parts ginseng, 180 parts cinnamon twig, 100 parts white peony root, 100 parts turmeric, 100 parts prepared licorice root, and 150 parts fresh ginger.

[0107] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 5 .

[0108] Example 19: 150 parts ginseng, 180 parts cinnamon twig, 200 parts white peony root, 100 parts turmeric, 30 parts prepared licorice root, and 150 parts fresh ginger.

[0109] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0110] Example 20: 150 parts ginseng, 180 parts cinnamon twig, 200 parts white peony root, 100 parts turmeric, 30 parts prepared licorice root, and 80 parts fresh ginger.

[0111] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0112] Example 21: 150 parts ginseng, 180 parts cinnamon twig, 200 parts white peony root, 100 parts turmeric, 100 parts prepared licorice root, and 80 parts fresh ginger.

[0113] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0114] Example 22: 150 parts ginseng, 180 parts cinnamon twig, 200 parts white peony root, 150 parts turmeric, 100 parts prepared licorice root, and 80 parts fresh ginger.

[0115] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0116] Example 23: 150 parts ginseng, 180 parts cinnamon twig, 200 parts white peony root, 100 parts turmeric, 100 parts prepared licorice root, and 20 parts fresh ginger.

[0117] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0118] Example 24: 80 parts ginseng, 150 parts cinnamon twig, 160 parts white peony root, 80 parts turmeric, 80 parts prepared licorice root, and 120 parts fresh ginger.

[0119] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0120] Example 25: 120 parts ginseng, 100 parts cinnamon twig, 160 parts white peony root, 80 parts turmeric, 80 parts prepared licorice root, and 120 parts fresh ginger.

[0121] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0122] Example 26: 10 parts ginseng, 150 parts cinnamon twig, 160 parts white peony root, 80 parts turmeric, 40 parts prepared licorice root, and 120 parts fresh ginger.

[0123] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0124] Example 27: 120 parts ginseng, 150 parts cinnamon twig, 160 parts white peony root, 80 parts turmeric, 80 parts prepared licorice root, and 50 parts fresh ginger.

[0125] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 6 .

[0126] Example 28: 120 parts ginseng, 150 parts cinnamon twig, 160 parts white peony root, 80 parts turmeric, 80 parts prepared licorice root, and 30 parts fresh ginger.

[0127] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 7 .

[0128] Example 29: 100 parts ginseng, 120 parts cinnamon twig, 130 parts white peony root, 65 parts turmeric, 60 parts prepared licorice root, and 90 parts fresh ginger.

[0129] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 7 .

[0130] Example 30: 100 parts ginseng, 120 parts cinnamon twig, 130 parts white peony root, 65 parts turmeric, 60 parts prepared licorice root, and 30 parts fresh ginger.

[0131] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 7 .

[0132] Example 31: 120 parts ginseng, 150 parts cinnamon twig, 160 parts white peony root, 80 parts turmeric, 80 parts prepared licorice root, and 30 parts fresh ginger.

[0133] Test results: This traditional Chinese medicine composition can achieve similar effects to fluoxetine, effectively improving depressive-like behaviors in CUMS and reducing sucrose preference rate. (See results below.) Figure 7 .

Claims

1. A traditional Chinese medicine composition for treating depression of the Qi deficiency and blood stasis type, characterized in that, By weight, the traditional Chinese medicine composition mainly consists of the following traditional Chinese medicines: Ginseng 25-400 parts, cinnamon twig 30-500 parts, white peony root 30-600 parts, turmeric 30-150 parts, prepared licorice root 30-150 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition also includes 20-200 parts of ginger.

3. The traditional Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 20-100 parts fresh ginger.

4. The traditional Chinese medicine composition according to claim 3, characterized in that: The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: 400 parts ginseng, 500 parts cinnamon twig, 600 parts white peony root, 150 parts turmeric, 150 parts prepared licorice root, and 50 parts fresh ginger.

5. The traditional Chinese medicine composition according to claim 1, characterized in that: The aforementioned traditional Chinese medicine composition comprises the following traditional Chinese medicines: 90 parts ginseng, 90 parts cinnamon twig, 150 parts white peony root, 100 parts turmeric, and 120 parts prepared licorice root.

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