Device and method for purifying siliceous sponge bone needle

Abstract

According to the purification equipment and method for the siliceous sponge spicules, the purification method based on strain preparation is adopted, and the prepared uniaxial siliceous sponge spicules are high in recovery rate, low in spicule breakage rate, high in purity and not prone to breakage; a gravity separation technology is adopted, so that large-scale industrial production can be realized; according to the freeze-drying equipment, a plurality of swing shafts are symmetrically arranged on a barrel cover, one side of each swing shaft is rotationally connected with the barrel cover, the other side of each swing shaft is rotationally arranged on a supporting piece, a plurality of supporting rods are fixedly arranged on the swing shafts and used for supporting a sponge disc, an impact table is fixed to the barrel cover to support part of the supporting rods, and a swing driving assembly is arranged on one side of each swing shaft. The swing driving assembly can drive the swing shaft to rotate by a certain angle and then fall off automatically, the supporting rods fall off along with the swing shaft and then impact the impact table, the sponge discs on the supporting rods vibrate, in this way, during freeze-drying, the vibrating sponge discs enable sponge to turn over to a certain degree, the freeze-drying effect is greatly improved, and the freeze-drying quality of the sponge is more uniform.

Description

Technical Field

[0001] This invention relates to sponge spicules, specifically to a purification device and method for silica sponge spicules. Background Technology

[0002] Modern scientific research has discovered that sponges contain a special type of skeletal structure, whose main components are silicon dioxide or calcium carbonate. Among them, siliceous spicules are formed by silicon proteases in siliceous sponges and have diverse structures, including common uniaxial spicules, tetraaxial spicules, triaxial spicules, and a type of bud bulb spicule.

[0003] Research on the mineralization mechanism and biomimetic applications of sponge spicules has revealed that their unique concentric microstructure and bio-silica composition endow them with both hardness and high toughness, making them promising for a wide range of applications. In recent years, the application of sponge spicules in the field of biomedical materials has increased significantly, leading to a surge in demand and a corresponding rise in raw material prices.

[0004] Currently, most traditional purification methods for sponge spicules include alkali treatment, oxidation, physical separation, and enzymatic hydrolysis. Among these, alkali treatment is simple to operate, low in cost, and suitable for large-scale production. It can effectively remove most organic impurities and achieve high purity. However, strong alkali may damage the surface structure of microneedles and affect their mechanical properties. Strict control of pH and temperature (usually 60-80℃) is required, otherwise the microneedles may dissolve. Residual alkali requires multiple water washes, which may increase environmental costs. Oxidation causes less damage to silica microneedles and is suitable for preserving the nanoscale surface pore structure. Hydrogen peroxide can sterilize simultaneously, reducing subsequent processing steps. However, oxidation is incomplete and often requires combination with alkali treatment or ultrasonic assistance. NaClO may introduce chlorine residue, affecting product safety. Physical separation methods are free of chemical reagents and are suitable for products with less stringent purity requirements. They can preserve the natural surface characteristics of microneedles (such as porous structures), but the purity is relatively low (60-80%), requiring repeated separations. They also require high uniformity in microneedle size, otherwise the efficiency is low. Enzymatic hydrolysis is a mild method (pH 7-8, 37-50℃), preserves the complete structure of microneedles, has high specificity, and avoids chemical residues. It is suitable for high-purity medical-grade applications, but enzyme costs are high, reaction time is long (requiring 24-72 hours), and multiple enzymes (such as protease + lipase) are required to completely remove impurities. The process is complex and may leave residual enzyme proteins, requiring subsequent inactivation treatment.

[0005] Therefore, providing a technology that can be easily industrialized without damaging the morphology of sponge spicules, while significantly improving the recovery rate and purity of spicules, has become an urgent problem for researchers to solve. Summary of the Invention

[0006] The purpose of this invention is to provide a purification device and method for silica sponge bone needles to solve the above-mentioned problems.

[0007] The above-mentioned technical objective of the present invention is achieved through the following technical solution: a method for purifying silica sponge bone spicules, comprising the following steps: Step 1: Soak the untreated sponge raw material in clean water, stir and let it settle, then filter out the mud and sand; after centrifugation and dehydration, freeze dry it using a freeze-drying device, and then pulverize it to obtain sponge powder.

[0008] Step 2: The obtained sponge powder is sterilized by high-pressure steam to obtain sponge fragments.

[0009] Step 3: Inoculate the working strain Streptomyces XL-34 into Gao's No. 1 medium and culture at 30℃ and 150 rpm for 24 hours until OD600=1.0. Mix the sponge fragments and bacterial suspension in a sterile container at a solid-liquid ratio of 1:10~20mL and shake well.

[0010] Step 4: Set the fermentation temperature to 30℃, adjust the pH to 7.2, and aerate the container containing the sponge fragments and working strain for 3-7 days. When the organic matter content is below the threshold, terminate the fermentation.

[0011] Step 5: Heat at 80℃ for 20 minutes to stop bacterial activity, and use ultrasonic cleaning to remove residual bacteria and decomposition products.

[0012] Step 6: Remove trace organic matter by calcining at 300℃ for 10 minutes, while retaining the inorganic framework.

[0013] Step 7: The product processed in step 6 is subjected to liquid phase gravity separation and dried to obtain uniaxial silica sponge bone needles.

[0014] The freeze-drying equipment for the purification method of the above-mentioned silica sponge bone needles includes a base, a bracket fixedly installed on the base, a freeze-drying barrel fixedly mounted on the bracket, and a first gas pipe and a second gas pipe fixedly connected to the freeze-drying barrel. The first gas pipe is used for vacuuming and is connected to an external vacuuming system. The second gas pipe is used for discharging and collecting water vapor formed by ice sublimation and is connected to an external vapor condensation and collection system. An evaporator is also installed at the bottom of the base. A lid is provided on one side of the freeze-drying barrel, the lid is used to close the freeze-drying barrel, a locking structure is provided between the lid and the freeze-drying barrel, a movable seat is fixed at the bottom of the lid, and rollers are installed at the bottom of the movable seat; Several swing shafts are symmetrically arranged on the bucket lid. One side of each swing shaft is rotatably connected to the bucket lid, and the other side is rotatably mounted on a support plate. Several support rods are fixedly mounted on the swing shafts to support the sponge discs. An impact platform is fixed on the bucket lid to support part of the support rods. A swing drive assembly is provided on one side of the swing shaft. The swing drive assembly can drive the swing shaft to rotate a certain angle and then fall off by itself. After the support rods fall off with the swing shafts, they impact the impact platform, causing the sponge discs on the support rods to vibrate. The vibrating sponge discs cause the sponge to flip.

[0015] Preferably, a vibrating head is provided through the impact platform, a spring is fixed between the vibrating head and the impact platform, the vibrating head is slidably connected to the impact platform, and one end of the vibrating head abuts against the support rod.

[0016] Preferably, a collection box is fixedly installed at the center of the bucket lid, the support plate is fixed to the collection box, the swing shaft is symmetrically arranged with the collection box as the center, a collection chamber is opened inside the collection box, a plurality of collection ports communicating with the collection chamber are symmetrically opened on the collection box, a guide plate fixed to the collection box is provided at the bottom of the collection port, each guide plate is mounted on the bottom of one of the swing shafts, and a discharge nozzle is provided at the bottom of the collection box.

[0017] Preferably, the oscillation drive assembly includes a rotating shaft fixed to one side of each of the oscillation axes, a gear fixed to one end of the rotating shaft, each of the gears meshing with a rack, a mating plate fixed between two racks, the mating plate being slidably disposed on the collection box, a cam being disposed on one side of the mating plate, the cam being fixed to a power shaft, the power shaft passing through the freeze-drying barrel and fixed to the motor shaft of the motor, the motor being fixed to a motor base, and the motor base being fixed to the base.

[0018] Preferably, at least two guide rods are fixed on the mating plate, each guide rod passing through a guide seat, and the guide seat is fixedly mounted on the collection box.

[0019] Preferably, two slide rails are symmetrically fixed on the base, a slider is slidably installed in the slide rail, a connecting strip is fixed on one side of the slider, and the connecting strip is fixed to the movable seat.

[0020] Preferably, an integrated electrical control box is fixedly mounted on the base.

[0021] In summary, the present invention has the following beneficial effects: 1. The purification method based on bacterial strain preparation yields uniaxial silica sponge spicules with high recovery rate, low breakage rate, high purity, and resistance to breakage.

[0022] 2. Gravity sorting technology is adopted, which enables large-scale industrial production.

[0023] 3. The freeze-drying equipment has several swing shafts symmetrically arranged on the lid. One side of the swing shaft is rotatably connected to the lid, and the other side is rotatably mounted on a support plate. Several support rods are fixed on the swing shafts to support the sponge trays. An impact platform is fixed on the lid to support some of the support rods. A swing drive assembly is set on one side of the swing shaft. The swing drive assembly can drive the swing shaft to rotate a certain angle and then fall off by itself. After the support rods fall with the swing shafts, they hit the impact platform, causing the sponge trays on the support rods to vibrate. In this way, during freeze-drying, the vibrating sponge trays will cause the sponge to turn over to a certain extent, which greatly improves the freeze-drying effect and makes the freeze-dried sponge quality more uniform.

[0024] 4. A vibrating head is installed through the impact platform, and a spring is fixed between the vibrating head and the impact platform. The vibrating head and the impact platform are slidably connected. One end of the vibrating head abuts against the support rod. The arrangement of the vibrating head and the spring ensures that when the support rod falls, it directly impacts the vibrating head. The vibrating head will descend and stretch the spring, thereby reducing the impact between the support rod and the impact platform. Replacing the spring with a different elastic coefficient can bring different impact effects, thereby changing the vibration effect on the sponge disc on the support rod to adapt to sponge particles of different sizes.

[0025] 5. The swing drive assembly includes a rotating shaft fixed on one side of each swing shaft, a gear fixed at one end of the rotating shaft, each gear meshing with a rack, a mating plate fixed between two racks, the mating plate being slidably mounted on the collection box, a cam being mounted on one side of the mating plate, the cam being fixed on the power shaft, the power shaft passing through the freeze-drying barrel and being fixed to the motor shaft of the motor, the motor being fixed on the motor base, and the motor base being fixed to the base. Manually push the mating plate downwards to rotate the shaft at a certain angle. The sponge disc on the support rod will tilt, and the sponge particles in the sponge disc will enter the collection port along the guide plate under their own gravity, and finally enter the collection chamber. The sponge particles in the collection chamber will be discharged from the discharge port. The user only needs to place a storage container at the bottom of the discharge port to collect all the freeze-dried sponge particles in the sponge disc at once, which is very convenient. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of the invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 This is a schematic diagram of the purification equipment. Figure 2This is a schematic diagram of the internal structure of the purification equipment; Figure 3 yes Figure 2 Enlarged view of point A in the middle; Figure 4 yes Figure 2 A sectional view; Figure 5 yes Figure 2 Side view; Figure 6 yes Figure 5 Enlarged view of point B in the middle; Figure 7 yes Figure 1 A sectional view.

[0028] In the diagram: 11. Base; 12. Movable seat; 14. Roller; 15. Bucket lid; 16. Slide rail; 17. Connecting bar; 18. Slider; 19. Integrated electrical control box; 20. Motor base; 21. Motor; 22. Power shaft; 23. Freeze-drying bucket; 24. No. 1 air pipe; 25. No. 2 air pipe; 26. Collection box; 27. Collection port; 28. Swing shaft; 29. ​​Support rod; 30. Impact platform; 31. Guide plate; 32. Collection chamber; 33. Discharge nozzle; 34. Mating plate; 35. Support plate; 36. Rotating shaft; 37. Gear; 38. Rack; 39. Guide rod; 40. Guide seat; 41. Vibrating head; 42. Spring; 43. Cam; 44. Locking structure. Detailed Implementation

[0029] A method for purifying silica sponge spicules includes the following steps: Example

[0030] Step 1: Soak the untreated sponge raw material in clean water 1-4 times, stir and settle, filter out the mud and sand, centrifuge and dehydrate, freeze dry it through a freeze-drying device, and then pulverize it to further destroy the organic matrix structure and obtain sponge powder. The sponge powder increases the surface area, which facilitates the penetration of microorganisms.

[0031] Step 2: The obtained sponge powder is subjected to high-pressure steam sterilization to obtain sponge fragments; the steam temperature is set to 101-133℃ and the sterilization time is 7-20 minutes.

[0032] Step 3: Inoculate the working strain Streptomyces XL-34 into Gao's No. 1 medium and culture at 30℃ and 150 rpm for 24 hours until OD600=1.0. Mix the sponge fragments and bacterial suspension in a sterile container at a solid-liquid ratio of 1:10~20mL and shake well.

[0033] Step 4: Set the fermentation temperature to 30℃, adjust the pH to 7.2 with NaOH / HCl, and aerate the container containing the sponge fragments and working strain (0.5-1 vvm). Ferment for 3-7 days (take daily samples to monitor the degradation rate). When the organic matter is below the threshold (≤0.05%), terminate the fermentation.

[0034] Step 5: Heat at 80℃ for 20 minutes to stop bacterial activity, and use ultrasonic cleaning to remove residual bacteria and decomposition products.

[0035] Step 6: Remove trace organic matter by calcining at 300℃ for 10 minutes, while retaining the inorganic framework.

[0036] Step 7: The product processed in Step 6 is subjected to liquid phase gravity separation. By adjusting the inclination angle of the separation bed and the flow rate of the flushing water, the impurities and bone needles are naturally distributed due to gravity and different particle sizes. The material from different regions is collected and dried to obtain a high-purity product.

[0037] The purified uniaxial silica sponge bone needles obtained had a purity of ≥99%, a breakage rate of less than 10%, and a recovery rate of 80%. Example

[0038] This embodiment provides a method for purifying silica sponge spicules according to the present invention. Compared with Example 1: The conditions for steps 1 and 2 are the same; In step 3, two strains, Streptomyces XL-34 and Bacillus subtilis, were inoculated. Streptomyces XL-34 was inoculated into Gao's No. 1 medium and cultured at 30°C with shaking at 150 rpm for 24 hours until OD600≈1.0. Bacillus subtilis was inoculated into LB medium and cultured at 30°C with shaking at 150 rpm for 24 hours until OD600≈1.0. The ratio of the two strains was 2:1. Digestion was carried out at a solid-liquid ratio of 1:20 mL for sponge fragments and bacterial solution, with all other conditions being the same. In step 4, the fermentation temperature is set to 32℃, the pH is adjusted to 7.0 using NaOH / HCl, and all other conditions remain the same. In step 5, heating at 90°C for 10 minutes terminates bacterial activity; The conditions for steps 6 and 7 are the same.

[0039] In this embodiment, the purified silica sponge bone needles obtained have a purity of ≥99%, a breakage rate of <15%, and a recovery rate of 87%.

[0040] See Figure 1-7This is a freeze-drying device used in the purification method of silica sponge bone needles, including a base 11, on which a bracket is fixedly installed. A freeze-drying tank 23 is fixedly mounted on the bracket. A first gas pipe 24 and a second gas pipe 25 are fixedly connected to the freeze-drying tank 23. The first gas pipe 24 is used for vacuuming and is connected to an external vacuuming system. The second gas pipe 25 is used to discharge and collect water vapor formed by ice sublimation and is connected to an external vapor condensation and collection system. An evaporator (not shown, as it is prior art and affects the illustration, and is consistent with conventional freeze-drying equipment) is also installed at the bottom of the base 11. A lid 15 is provided on one side of the freeze-drying barrel 23. The lid 15 is used to close the freeze-drying barrel 23. A locking structure 44 is provided between the lid 15 and the freeze-drying barrel 23. The locking structure 44 is used to lock and unlock the lid 15 and the freeze-drying barrel 23 (the locking structure can be used according to actual needs, and any conventional locking structure in the art can be used in this application). A movable seat 12 is fixed at the bottom of the lid 15. A roller 14 is installed at the bottom of the movable seat 12. After unlocking, the lid 15 and the freeze-drying barrel 23 have a pull-out design, which makes it convenient to take out and put in the sponge tray. A plurality of swing shafts 28 are symmetrically arranged on the lid 15. One side of each swing shaft 28 is rotatably connected to the lid 15, and the other side of each swing shaft 28 is rotatably mounted on a support plate 35. A plurality of support rods 29 are fixedly mounted on the swing shafts 28. The support rods 29 are used to support the sponge trays. An impact platform 30 is fixed on the lid 15 to support part of the support rods 29. A swing drive assembly is provided on one side of the swing shaft 28. The swing drive assembly can drive the swing shaft 28 to rotate a certain angle and then fall off by itself. After the support rods 29 fall off with the swing shafts 28, they impact the impact platform 30, causing the sponge trays on the support rods 29 to vibrate. In this way, during freeze-drying, the vibrating sponge trays will cause the sponge to turn over to a certain extent, greatly improving the freeze-drying effect and making the freeze-dried quality of the sponge more uniform. Specifically, a vibrating head 41 is provided through the impact platform 30, and a spring 42 is fixed between the vibrating head 41 and the impact platform 30. The vibrating head 41 is slidably connected to the impact platform 30, and one end of the vibrating head 41 abuts against the support rod 29. The arrangement of the vibrating head 41 and the spring 42 allows the support rod 29 to fall and directly impact the vibrating head 41. The vibrating head 41 will descend and stretch the spring 42, thereby mitigating the impact between the support rod 29 and the impact platform 30. Replacing the spring 42 with different elastic coefficients can bring different impact effects, thereby changing the vibration effect on the sponge disc on the support rod 29 to adapt to sponge particles of different sizes. A collection box 26 is fixedly installed at the center of the bucket lid 15. The support piece 35 is fixed to the collection box 26. The swing shaft 28 is symmetrically arranged with the collection box 26 as the center. A collection chamber 32 is opened inside the collection box 26. Several collection ports 27 communicating with the collection chamber 32 are symmetrically opened on the collection box 26. A guide plate 31 fixed to the collection box 26 is provided at the bottom of the collection port 27. Each guide plate 31 is mounted on the bottom of one of the swing shafts 28. A discharge nozzle 33 is provided at the bottom of the collection box 26. The oscillation drive assembly includes a rotating shaft 36 fixed to one side of each of the oscillation shafts 28. A gear 37 is fixed to one end of each rotating shaft 36, and each gear 37 meshes with a rack 38. A mating plate 34 is fixed between two racks 38. The mating plate 34 is slidably mounted on the collection box 26. A cam 43 is provided on one side of the mating plate 34 and is fixed to a drive shaft 22. The drive shaft 22 passes through the freeze-drying barrel 23 and is fixed to the motor shaft of a motor 21. The motor 21 is fixed to a motor base 20, which is fixed to the base 11. When the motor 21 is started, it drives the drive shaft 22 to rotate, and the drive shaft 22 drives the cam 43. 3. When the cam 43 rotates, it pushes the mating plate 34 to descend until the cam 43 disengages from the mating plate 34. When the mating plate 34 descends, it drives the rack 38 to descend. The meshing of the rack 38 and the gear 37 drives the rotating shaft 36 and the swing shaft 28 to rotate. The swing shaft 28 drives the support rod 29 and the sponge disc on the support rod 29 to rotate. After the cam 43 disengages from the mating plate 34, the sponge disc on the support rod 29 falls off under its own weight, causing the support rod 29 and the swing shaft 28 to rotate. The support rod 29 collides with the impact platform 30, causing the sponge particles in the sponge disc to tumble. At least two guide rods 39 are fixed on the mating plate 34, and each guide rod 39 passes through the guide seat 40, which is fixedly mounted on the collection box 26.

[0041] Two slide rails 16 are symmetrically fixed on the base 11. A slider 18 is slidably installed in the slide rail 16. A connecting strip 17 is fixed to one side of the slider 18 and is fixed to the movable seat 12. After the bucket lid 15 is manually pulled out, the mating plate 34 is manually pushed downward, causing the rotating shaft 36 to rotate at a certain angle. The sponge tray on the support rod 29 will tilt. The sponge particles in the sponge tray will enter the collection port 27 along the guide plate 31 under their own gravity and eventually enter the collection chamber 32. The sponge particles in the collection chamber 32 will be discharged from the discharge nozzle 33. The user only needs to place a storage container at the bottom of the discharge nozzle 33 to collect all the freeze-dried sponge particles in the sponge tray at once, which is very convenient. After collection, the empty sponge tray can be manually removed.

[0042] An integrated electrical control box 19 is fixedly installed on the base 11 to control the operation of each electrical appliance and provide power.

[0043] Working principle of freeze-drying equipment: Manually pull out the bucket lid 15, place a sponge tray containing wet sponge particles on the support rod 29, manually reset the bucket lid 15 and lock it through the locking structure 44, and then perform the freeze-drying operation after locking. During the freeze-drying process, the motor 21 is started by the integrated electrical control box 19. The motor 21 drives the power shaft 22 to rotate, and the power shaft 22 drives the cam 43 to rotate. When the cam 43 rotates, it pushes the mating plate 34 to descend until the cam 43 disengages from the mating plate 34. When the mating plate 34 descends, it drives the rack 38 to descend. The meshing of the rack 38 and the gear 37 drives the rotating shaft 36 and the swing shaft 28 to rotate. The swing shaft 28 drives the support rod 29 and the sponge disc on the support rod 29 to rotate. After the cam 43 disengages from the mating plate 34, the sponge disc on the support rod 29 falls off under its own weight, causing the support rod 29 and the swing shaft 28 to rotate. The support rod 29 collides with the impact platform 30, causing the sponge particles in the sponge disc to tumble. After freeze-drying is complete, turn off the motor 21, manually unlock the locking structure 44, pull out the bucket lid 15, manually push the mating plate 34 downward, causing the rotating shaft 36 to rotate at a certain angle. The sponge tray on the support rod 29 will tilt, and the sponge particles in the sponge tray will enter the collection port 27 along the guide plate 31 under their own gravity, and finally enter the collection chamber 32. The sponge particles that enter the collection chamber 32 will be discharged from the discharge nozzle 33. The user only needs to place a storage container at the bottom of the discharge nozzle 33 to collect all the freeze-dried sponge particles in the sponge tray at once.

[0044] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand and implement the present invention. They should not be construed as limiting the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A method for purifying silica sponge spicules, characterized in that, Includes the following steps: (1): Soak the sponge raw material in water, stir and let it settle, then filter out the mud and sand; after centrifugation and dehydration, freeze dry it using a freeze-drying device, and then pulverize it to obtain sponge powder; (2): The obtained sponge powder was sterilized by high-pressure steam to obtain sponge fragments; (3): Inoculate Streptomyces XL-34 into Gao's No. 1 medium and shake to culture until OD600=1.

0. Mix the sponge fragments and bacterial suspension in a sterile container at a solid-liquid ratio of 1:10~20mL. (4): Aerate the container for fermentation; (5): Heating stops bacterial activity, and ultrasonic cleaning removes residual bacteria and decomposition products; (6): Calcination removes trace organic matter while retaining the inorganic framework; (7): The product treated by (6) was subjected to liquid phase gravity separation and dried to obtain uniaxial silica sponge bone needles.

2. A freeze-drying apparatus for the purification method of silica sponge bone needles according to claim 1, comprising a base (11), a bracket fixedly installed on the base (11), a freeze-drying barrel (23) fixedly mounted on the bracket, a first gas pipe (24) and a second gas pipe (25) fixedly connected on the freeze-drying barrel (23), the first gas pipe (24) being used for vacuuming and connected to an external vacuuming system, the second gas pipe (25) being used for discharging and collecting water vapor formed by ice sublimation and connected to an external vapor condensation and collection system, and an evaporator also installed at the bottom of the base (11), characterized in that: A lid (15) is provided on one side of the freeze-drying barrel (23). The lid (15) is used to close the freeze-drying barrel (23). A locking structure (44) is provided between the lid (15) and the freeze-drying barrel (23). A movable seat (12) is fixed at the bottom of the lid (15). A roller (14) is installed at the bottom of the movable seat (12). A plurality of swing shafts (28) are symmetrically arranged on the lid (15). One side of the swing shaft (28) is rotatably connected to the lid (15), and the other side of the swing shaft (28) is rotatably arranged on the support plate (35). A plurality of support rods (29) are fixedly arranged on the swing shaft (28). The support rods (29) are used to support the sponge discs. An impact platform (30) is fixed on the lid (15) to support part of the support rods (29). A swing drive assembly is arranged on one side of the swing shaft (28). The swing drive assembly can drive the swing shaft (28) to rotate and then fall off by itself. After the support rods (29) fall with the swing shaft (28), they hit the impact platform (30), causing the sponge discs on the support rods (29) to vibrate. The vibrating sponge discs will cause the sponge to flip.

3. The freeze-drying equipment according to claim 2, characterized in that: A vibrating head (41) is provided through the impact platform (30), and a spring (42) is fixed between the vibrating head (41) and the impact platform (30). The vibrating head (41) is slidably connected to the impact platform (30), and one end of the vibrating head (41) abuts against the support rod (29).

4. The freeze-drying equipment according to claim 3, characterized in that: A collection box (26) is fixedly installed at the center of the bucket lid (15). The support plate (35) is fixed to the collection box (26). The swing shaft (28) is symmetrically arranged with the collection box (26) as the center. A collection chamber (32) is opened inside the collection box (26). Several collection ports (27) communicating with the collection chamber (32) are symmetrically opened on the collection box (26). A guide plate (31) fixed to the collection box (26) is provided at the bottom of the collection port (27). Each guide plate (31) is mounted on the bottom of one of the swing shafts (28). A discharge nozzle (33) is provided at the bottom of the collection box (26).

5. The freeze-drying equipment according to claim 4, characterized in that: The swing drive assembly includes a rotating shaft (36) fixed to one side of each swing shaft (28), a gear (37) fixed to one end of the rotating shaft (36), each gear (37) meshing with a rack (38), a mating plate (34) fixed between the two racks (38), the mating plate (34) being slidably disposed on the collection box (26), a cam (43) being disposed on one side of the mating plate (34), the cam (43) being fixed on a power shaft (22), the power shaft (22) passing through the freeze-drying barrel (23) and being fixed to the motor shaft of the motor (21), the motor (21) being fixed on a motor base (20), and the motor base (20) being fixed to the base (11).

6. The freeze-drying equipment according to claim 5, characterized in that: At least two guide rods (39) are fixed on the mating plate (34), each of the guide rods (39) passing through the guide seat (40), the guide seat (40) being fixedly mounted on the collection box (26).

7. The freeze-drying equipment according to claim 2, characterized in that: Two slide rails (16) are symmetrically fixed on the base (11). A slider (18) is slidably installed in the slide rail (16). A connecting strip (17) is fixed on one side of the slider (18). The connecting strip (17) is fixed to the movable seat (12).

8. The freeze-drying equipment according to claim 2, characterized in that: An integrated electrical control box (19) is fixedly installed on the base (11).

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