A Pasteurella multocida, its construction method and application

By knocking out the β-glucan synthesis gene gas1 in the cell wall of Pichia pastoris GS115, the cell size was reduced, solving the problem of low efficiency of intracellular product secretion in yeast in the existing technology. This achieved efficient product secretion and synthesis, simplified the operation process, and improved fermentation production efficiency.

CN120944724BActive Publication Date: 2026-06-30广州华酵生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州华酵生物科技有限公司
Filing Date
2025-07-03
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies have not yet shown the effect of Pichia pastoris GS115 cell size on intracellular product secretion, and traditional methods such as adding permeation enhancers are cumbersome and prone to cell death, making it difficult to effectively improve the secretion efficiency of yeast intracellular products.

Method used

By knocking out gas1, a gene related to β-glucan synthesis in the cell wall of Pichia pastoris, cell size was reduced, and Pichia pastoris GS115Δgas1 was constructed. This reduced the transfer distance from the cell surface to the cell nucleus and improved oxygen transfer and product secretion.

Benefits of technology

This method achieved stable reduction in the cell size of Pichia pastoris, improved the secretion rate and synthesis efficiency of intracellular products, simplified the operation process, enhanced cell membrane permeability, and improved fermentation production efficiency.

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Abstract

This application relates to the field of biotechnology, specifically disclosing a *Pichia pastoris* yeast, its construction method, and its applications. This application involves knocking out the *gas1* gene, which is related to β-glucan synthesis, in *Pichia pastoris*. By deleting the *gas1* gene, which encodes β-glucan in the cell wall of *Pichia pastoris*, this application reduces cell size, decreases the transfer distance from the cell surface to the nucleus, improves oxygen transfer and product secretion during high-density culture, and enhances fermentation efficiency. Furthermore, the *Pichia pastoris* yeast constructed using this application enhances the synthesis of the intracellular product bisabolol and also enhances its secretion capacity. This application, by reducing cell size and decreasing the carbon flow to cell wall polysaccharide synthesis while increasing the carbon source flow to target product synthesis, has significant implications for industrial production.
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