Bamboo reed seedling domestication substrate and efficient bamboo reed domestication seedling raising method

Abstract

The invention provides a bamboo reed seedling domestication substrate and an efficient bamboo reed domestication seedling raising method, and belongs to the technical field of plant cultivation, the bamboo reed seedling domestication substrate comprises humus soil, perlite, organic fertilizer, a heading accelerant, nitrogen-fixing bacteria and bamboo reed charcoal, and the heading accelerant comprises KH2PO4, MgSO4. 7H2O and CaSO4. 2H2O. According to the method, the nitrogen-fixing microbial inoculum and the bamboo reed charcoal are added into the domesticated seedling substrate of the bamboo reed at the same time, the rich pore structure and the high specific surface area of the bamboo reed charcoal can provide a good parasitic environment for nitrogen-fixing bacteria, the pore structure of the bamboo reed charcoal can protect the nitrogen-fixing bacteria from being interfered by the external environment, and meanwhile, a continuous and balanced moisture and nutrient environment is created for the nitrogen-fixing bacteria. The balling accelerant can enhance the viscosity stability of exopolysaccharides secreted by nitrogen-fixing bacteria, increase the adhesion to soil particles and improve the balling degree of the matrix.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant cultivation, in particular to a reed canary grass seedling domestication substrate and an efficient reed canary grass seedling domestication seedling raising method. BACKGROUND

[0002] Reed canary grass (Phalaris arundinacea L.) is a perennial herbaceous energy plant with strong environmental adaptability and wide distribution, which can be planted on large scale in abandoned marginal land. The harvested stems and stalks have rich application scenarios and can be used for power generation, pressed wood, and paper pulp. They can also be processed into hydrogen, natural gas, and biochar through downstream processing technology, and have become an energy plant that is widely concerned at present. Reed canary grass cannot harvest seeds with reproductive ability and can only be propagated through asexual reproduction. At present, the main way to obtain seedlings is to use plant tissue culture technology to obtain seedlings on a large scale. Arundo donax L. The tissue culture seedlings of reed canary grass must go through a domestication process before they can be planted in the field. Improving the domestication efficiency and obtaining domestication seedlings with strong tolerance are very important for improving the survival rate of field planting.

[0003] However, the substrate formula used for conventional reed canary grass seedling domestication and seedling raising has the following problems: (1) the root system growth and balling are unstable, and long-distance or improper transportation can easily cause the root system substrate to fall off, ultimately leading to a decrease in the survival rate of field planting; (2) the planting site of reed canary grass is mostly marginal land, and the soil is deficient in nutrients; (3) the traditional substrate domestication time is long. SUMMARY

[0004] In view of the technical problems in the background art, the present application provides a reed canary grass seedling domestication substrate and an efficient reed canary grass seedling domestication seedling raising method, which aims to solve the technical problems of low survival rate of reed canary grass domestication seedlings due to long-distance transportation and poor planting site, and long domestication time.

[0005] In a first aspect, the embodiments of the present application provide a reed canary grass seedling domestication substrate, which comprises humus soil, perlite, organic fertilizer, balling promoting agent, nitrogen-fixing bacteria, and reed canary grass charcoal. The balling promoting agent comprises KH2PO4, MgSO4·7H2O, and CaSO4·2H2O.

[0006] In some embodiments, the substrate comprises 60 parts by mass of humus soil, 20 parts by mass of perlite, 15 parts by mass of organic fertilizer, 1-3 parts by mass of balling promoting agent, 2-4 parts by mass of nitrogen-fixing bacteria, and 1-5 parts by mass of reed canary grass charcoal.

[0007] In some embodiments, the mass ratio of KH2PO4, MgSO4·7H2O, and CaSO4·2H2O in the balling promoting agent is 1:1:1.

[0008] In some embodiments, the reed canary grass charcoal is obtained by drying and crushing reed canary grass stems and stalks and then performing high-temperature anaerobic pyrolysis. The high-temperature pyrolysis temperature is 800-900℃, and the time is 6-9s.

[0009] In some embodiments, the specific surface area of ​​bamboo charcoal is 205.5~216.5 m². 3 / g, with a fixed carbon content of 68.5%–76.5%.

[0010] In some embodiments, the nitrogen-fixing bacteria are Azotobacter chrysozoans.

[0011] Secondly, embodiments of this application provide an efficient method for cultivating domesticated Reed truncatum seedlings, comprising the following steps: After pretreatment and cleaning, the tissue culture seedlings of Reed are used to obtain single tissue culture seedlings. Single tissue culture seedlings were transplanted into a substrate and cultured to obtain acclimatized seedlings. The substrate consists of 60 parts humus, 20 parts perlite, 15 parts organic fertilizer, 1-3 parts ball-forming promoter, 2-4 parts nitrogen-fixing bacteria, and 1-5 parts reed charcoal. The substrate pH is adjusted to 6.5-7.5, and the carbon-nitrogen ratio of the substrate is (40-70):1.

[0012] In some embodiments, the pretreatment step includes: keeping the Reed tissue culture bottle containing the Reed tissue culture seedlings unopened and culturing it for 10 days under natural light at 20-30°C.

[0013] In some embodiments, the cleaning step includes: cleaning the roots of the pretreated Reed tissue culture seedlings, dividing them into individual plants, cutting the root length to 2-3 cm and the plant height to 6-7 cm, to obtain individual tissue culture seedlings.

[0014] In some embodiments, single tissue culture seedlings are soaked in 0.1% to 0.2% carbendazim for 15 minutes.

[0015] In some embodiments, the cultivation steps include: culturing under light for 10-14 hours at a temperature of 25-30°C and a substrate humidity of 70% of its maximum water holding capacity, with a light intensity of 5000-10000 lx, followed by culturing in the dark for 10-14 hours.

[0016] In some embodiments, lime is used to adjust the pH of the matrix to 6.5-7.5.

[0017] The advantages of this application, which differ from existing technical solutions, include: 1. This invention adds nitrogen-fixing bacteria to the substrate, which can rapidly multiply to form a highly vigorous bacterial community. On the one hand, this community absorbs nitrogen from the air to form ecological nitrogen fertilizer, increasing soil fertility and improving fertilizer utilization. Simultaneously, it improves the ecological structure around the root system, thereby promoting crop growth. On the other hand, nitrogen-fixing bacteria can secrete polysaccharides, promoting the aggregation of soil microparticles to form a stable granular structure, increasing the substrate's balling density. This granular structure increases soil porosity, improves aeration and water permeability, enhances water and fertilizer retention capacity, and reduces soil erosion. This invention also adds balling promoters to the substrate, including KH2PO4, MgSO4·7H2O, and CaSO4·2H2O. KH2PO4 provides a phosphorus source and helps maintain a stable pH in the substrate, which is conducive to the secretion of more extracellular polysaccharides by nitrogen-fixing bacteria. The CaSO4·2H2O and MgSO4·7H2O... 2+ Mg 2+ Ions can bind to extracellular polysaccharides secreted by nitrogen-fixing bacteria to form stable ion bridges, thereby improving the adhesion of extracellular polysaccharides to soil particles and enhancing their stability, which in turn improves the bulking of the matrix.

[0018] 2. The *Arundinaria spp.* charcoal produced by the anaerobic pyrolysis process in this invention has rich and uniform porosity, a large specific surface area, and abundant organic matter, which can improve soil aeration and enhance fertility. This invention aims to simultaneously add nitrogen-fixing bacteria and *Arundinaria spp.* charcoal to the substrate of *Arundinaria spp.* acclimatized seedlings. The rich pore structure and high specific surface area of ​​*Arundinaria spp.* charcoal can provide a good habitat and parasitic environment for nitrogen-fixing bacteria. These pore structures can protect nitrogen-fixing bacteria from external environmental interference, while creating a continuous and balanced supply of water and nutrients for them.

[0019] 3. This invention uses a combination of nitrogen-fixing bacteria, a bulb-forming promoter, and reed charcoal, and optimizes the acclimatization conditions. It controls the pH, water holding capacity, and acclimatization temperature of the substrate to improve the survival rate and growth of acclimatized seedlings, increase the survival rate in the field, reduce the cost of seedlings, and establish a standardized and efficient acclimatization system for reeds.

[0020] The above description is only an overview of the technical solution of this application. In order to better understand the technical means of this application and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this application more obvious and understandable, the following are specific embodiments of this application. Detailed Implementation

[0021] The embodiments of the technical solution of this application will be described in detail below. The following embodiments are only used to illustrate the technical solution of this application more clearly, and are therefore only examples, and should not be used to limit the scope of protection of this application.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms “comprising” and “having”, and any variations thereof, in the specification and claims of this application are intended to cover non-exclusive inclusion.

[0023] The large-scale propagation of Reed hyacinth seedlings is currently mainly carried out through tissue culture technology. The acclimatization of tissue culture seedlings before planting is a key step. However, conventional substrate acclimatization currently suffers from low acclimatization efficiency and uneven growth.

[0024] To address the technical challenges of low survival rates and long acclimatization times for Arundo donax seedlings due to long-distance transportation and poor planting conditions, this application provides an acclimatization substrate and an efficient method for cultivating Arundo donax seedlings. By adding nitrogen-fixing bacteria and Arundo donax charcoal to the substrate, the addition of nitrogen-fixing bacteria during the acclimatization stage increases the substrate's nitrogen-fixing bacteria content, improving the survival rate of Arundo donax seedlings and soil nutrient utilization efficiency during planting. Simultaneously, Arundo donax charcoal provides a certain amount of space for the nitrogen-fixing bacteria to multiply, increasing the bacterial content. The combined use of these two materials in the base substrate shortens the acclimatization time and significantly improves root head formation. This facilitates long-distance transportation and provides initial nutrition for seedlings planted in poor marginal land, greatly improving the survival rate of field-grown seedlings.

[0025] In a first aspect, embodiments of this application provide a domestication substrate for reed seedlings, comprising humus, perlite, organic fertilizer, a ball-forming promoter, nitrogen-fixing bacteria, and reed charcoal. The ball-forming promoter includes KH2PO4, MgSO4·7H2O, and CaSO4·2H2O.

[0026] In some embodiments, by weight, the composition includes 60 parts humus, 20 parts perlite, 15 parts organic fertilizer, 1-3 parts ball-forming promoter, 2-4 parts nitrogen-fixing bacteria, and 1-5 parts reed charcoal.

[0027] In some embodiments, the mass ratio of KH2PO4, MgSO4·7H2O and CaSO4·2H2O in the ball-forming promoter is 1:1:1.

[0028] In the technical solution of this application embodiment, nitrogen-fixing bacteria agent and arisaema charcoal are added simultaneously to the domestication seedling substrate of reed. The rich pore structure and high specific surface area of ​​reed charcoal can provide a good parasitic environment for nitrogen-fixing bacteria. Its pore structure can protect nitrogen-fixing bacteria from interference from the external environment, while creating a continuous and balanced water and nutrient environment for nitrogen-fixing bacteria.

[0029] This invention further incorporates a pelleting promoter into the matrix, comprising KH₂PO₄, MgSO₄·7H₂O, and CaSO₄·2H₂O. KH₂PO₄ provides a phosphorus source and helps stabilize the pH of the matrix; the CaSO₄·2H₂O and MgSO₄·7H₂O contain Ca... 2+ Mg 2+ Ions can bind to extracellular polysaccharides secreted by nitrogen-fixing bacteria, forming stable ion bridges and further improving the bulb formation of the substrate. Higher bulb formation can improve the survival rate of Arundo donax seedlings planted in the field, because Arundo donax seedlings need to be transported over long distances to the planting site. If the bulb formation is poor, a large amount of substrate will fall off during transportation, resulting in exposed roots and affecting the survival rate.

[0030] In some embodiments, reed charcoal is obtained by drying and crushing reed stems and then subjecting them to high-temperature anaerobic pyrolysis. The high-temperature pyrolysis temperature is 800~900℃, and the time is 6~9s.

[0031] In some embodiments, the specific surface area of ​​bamboo charcoal is 205.5~216.5 m². 3 / g, with a fixed carbon content of 68.5%–76.5%.

[0032] In the technical solution of this application embodiment, the reed charcoal used in this invention is obtained from Wuhan Lando Biotechnology Co., Ltd., in powder form, and is obtained by drying and crushing reed stems and then using an anaerobic pyrolysis process. The biochar after high-temperature pyrolysis has a distinct and regular pore structure, with dense and well-developed pores.

[0033] In some embodiments, the nitrogen-fixing bacteria are Azotobacter chrysozoans.

[0034] In the technical solution of this application embodiment, *Azotobacter chrysophagus* can absorb nitrogen from the air and rapidly multiply in the substrate to become the dominant bacterial group. As a free-living nitrogen-fixing bacterium, it does not need to live in symbiosis with plants; the dead bacteria provide a large amount of nitrogen fertilizer to the plants. Adding *Azotobacter chrysophagus* agent to the substrate for *Arundo donax* acclimatization can promote the survival, acclimatization, and growth of acclimatized seedlings. Choosing an appropriate dosage can, on the one hand, allow it to quickly become the dominant bacterial group, and on the other hand, save costs while meeting the requirements. Under the conditions of this invention, it is preferable to add 3 parts of bacterial amount.

[0035] During the process of associating nitrogen fixation, Azotobacter chrysotrichum secretes viscous substances such as polysaccharides. These polysaccharides help it form a biofilm on the root surface or root cap of plants, thereby enhancing its interaction with plants and nitrogen fixation efficiency.

[0036] The Azotobacter chrysophyte used in this invention was purchased from Herrenknecht Biotechnology Co., Ltd. of Yangzhou City (supervised by Harbin Institute of Technology). It is in powder form with a live bacteria content of ≥10 billion / gram and is uniformly mixed into the matrix according to the dosage.

[0037] Secondly, embodiments of this application provide an efficient method for cultivating domesticated Reed truncatum seedlings, comprising the following steps: After pretreatment and cleaning, the tissue culture seedlings of Reed are used to obtain single tissue culture seedlings. Single tissue culture seedlings were transplanted into a substrate and cultured to obtain acclimatized seedlings. The substrate consists of 60 parts humus, 20 parts perlite, 15 parts organic fertilizer, 1-3 parts ball-forming promoter, 2-4 parts nitrogen-fixing bacteria, and 1-5 parts reed charcoal. The substrate pH is adjusted to 6.5-7.5, and the carbon-nitrogen ratio of the substrate is (40-70):1.

[0038] In the technical solution of this application embodiment, it is necessary to control the carbon-nitrogen ratio of the substrate to be no less than (40~70):1, otherwise the nitrogen fixation function of nitrogen-fixing bacteria will be inhibited, and a soil environment with high carbon and low nitrogen is conducive to its nitrogen fixation function.

[0039] The organic fertilizer brand selected is Mengyang Runtu, with organic matter ≥30% and N+P+K ≥4%.

[0040] In some embodiments, the pretreatment step includes: keeping the cap of the Arundinaria tissue culture bottle containing Arundinaria tissue culture seedlings closed and culturing it for 10 days under natural light at 20-30°C.

[0041] In the technical solution of this application embodiment, the tissue culture bottles of Reed are placed in an acclimatization shed, and the culture conditions are changed from sterile to septic, and from constant temperature to variable temperature, so that the tissue culture seedlings gradually adapt to the external environment and improve their resistance. The temperature in the acclimatization shed needs to be controlled at 25~30℃. When the temperature is below 10℃ or above 40℃, the growth of nitrogen-fixing bacteria will be inhibited.

[0042] In some embodiments, the cleaning step includes: cleaning the roots of the pretreated Reed tissue culture seedlings, dividing them into individual plants, cutting the root length to 2-3 cm and the plant height to 6-7 cm, to obtain individual tissue culture seedlings.

[0043] In the technical solution of this application embodiment, the purpose of pruning the roots is to facilitate transplanting, and the purpose of pruning the height is to reduce transpiration and improve the survival rate.

[0044] In the technical solution of this application embodiment, the roots of the pretreated Reed tissue culture seedlings are cleaned to remove the agar from the roots.

[0045] In some embodiments, single tissue culture seedlings are soaked in 0.1% to 0.2% carbendazim for 15 minutes.

[0046] In the technical solution of this application embodiment, carbendazim is used for soaking to remove miscellaneous bacteria.

[0047] In some embodiments, the cultivation steps include: culturing under light for 10-14 hours at a temperature of 25-30°C and a substrate humidity of 70% of its maximum water holding capacity, with a light intensity of 5000-10000 lx, followed by culturing in the dark for 10-14 hours.

[0048] In some embodiments, lime is used to adjust the pH of the matrix to 6.5-7.5.

[0049] In the technical solution of this application embodiment, nitrogen-fixing bacteria are sensitive to soil pH. Under the conditions of this invention, the acclimatization effect is best at around pH 7.0. Excessive acidity or alkalinity will inhibit the activity of nitrogen-fixing bacteria.

[0050] The following are some specific embodiments. It should be noted that the embodiments described below are exemplary and are only used to explain this application, and should not be construed as limiting this application. Where specific techniques or conditions are not specified in the embodiments, they shall be performed in accordance with the techniques or conditions described in the literature in this field or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be obtained commercially.

[0051] I. Preparation Method Example 1 An efficient method for cultivating domesticated Reed saplings includes the following steps: Place the tissue culture bottles containing the aforementioned Reed Tissue Culture seedlings directly into the greenhouse storage area without opening the caps, control the greenhouse temperature at 25~30℃, and cultivate under natural light for ten days.

[0052] After placing the Reed tissue culture bottles in the greenhouse for ten days, open the caps and take out the Reed tissue culture seedlings. Thoroughly clean the agar from the roots, divide them into individual plants, cut the root length to 2-3cm, and the plant height to 6-7cm. Soak them in 0.1% carbendazim for 15 minutes to obtain individual tissue culture seedlings for later use.

[0053] Mix 60 parts humus, 20 parts perlite, 15 parts organic fertilizer, 1 part each of KH2PO4, MgSO4·7H2O and CaSO4·2H2O (both KH2PO4 and CaSO4·2H2O), 2 parts nitrogen-fixing bacteria, and 1 part reed charcoal thoroughly to obtain the substrate. Adjust the pH to 7.0 with lime and control the carbon-to-nitrogen ratio of the substrate to 55:1. Spread the substrate evenly in the seedling trays and water thoroughly.

[0054] Transplant individual tissue culture seedlings into seedling trays, provide initial watering, and then add more substrate on top. Place the substrate trays containing the transplanted seedlings in a greenhouse, controlling the temperature at 25-30℃, and water regularly to ensure the substrate moisture content is 70% of its maximum water holding capacity. Incubate for 14 hours in light and 10 hours in darkness, with a light intensity of 5000-10000 lx. After cultivation, you will obtain *Arundo donax* acclimatized seedlings.

[0055] Example 2 An efficient method for cultivating domesticated Reed saplings includes the following steps: Place the tissue culture bottles containing the aforementioned Reed Tissue Culture seedlings directly into the greenhouse storage area without opening the caps, control the greenhouse temperature at 25~30℃, and cultivate under natural light for ten days.

[0056] After placing the Reed tissue culture bottles in the greenhouse for ten days, open the caps and take out the Reed tissue culture seedlings. Thoroughly clean the agar from the roots, divide them into individual plants, cut the root length to 2-3cm, and the plant height to 6-7cm. Soak them in 0.1% carbendazim for 15 minutes to obtain individual tissue culture seedlings for later use.

[0057] Mix 60 parts humus, 20 parts perlite, 15 parts organic fertilizer, 1 part each of KH2PO4, MgSO4·7H2O and CaSO4·2H2O (both KH2PO4 and CaSO4·2H2O), 2 parts nitrogen-fixing bacteria, and 1 part reed charcoal thoroughly to obtain the substrate. Adjust the pH to 7.0 with lime and control the carbon-to-nitrogen ratio of the substrate to 55:1. Spread the substrate evenly in the seedling trays and water thoroughly.

[0058] Transplant individual tissue culture seedlings into seedling trays, provide initial watering, and then add more substrate on top. Place the substrate trays containing the transplanted seedlings in a greenhouse, controlling the temperature at 25-30℃, and water regularly to ensure the substrate moisture content is 70% of its maximum water holding capacity. Incubate for 14 hours in light and 10 hours in darkness, with a light intensity of 5000-10000 lx. After cultivation, you will obtain *Arundo donax* acclimatized seedlings.

[0059] Example 3 The difference between Example 2 and Example 1 is that the amount of nitrogen-fixing bacteria added is 4 parts, while the other steps are the same.

[0060] Example 4 The difference between Example 4 and Example 1 is that lime was used to adjust the pH of the matrix to 6.5, while the other steps are the same.

[0061] Example 5 The difference between Example 5 and Example 1 is that lime was used to adjust the pH of the matrix to 7.5, while the other steps are the same.

[0062] Example 6 The difference between Example 6 and Example 1 is that the amount of reed charcoal added is 3 parts, while the other steps are the same.

[0063] Example 7 The difference between Example 7 and Example 1 is that the amount of reed charcoal added is 5 parts, while the other steps are the same.

[0064] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that no nitrogen-fixing bacteria were added, while all other steps were the same.

[0065] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that no reed charcoal was added, but all other steps were the same.

[0066] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that no balling promoter was added, but all other steps were the same.

[0067] II. Testing Methods Each embodiment and comparative example had three parallel groups, with two seed trays (50 wells × 2) in each group. After 40 days of cultivation, the number of surviving plants was counted, and the survival rate was calculated. The height of 20 individual plants in each group was measured, and the average plant height was obtained by taking the average value. Growth was recorded, with stronger growth being more desirable. More and more.

[0068] Method for testing the degree of balling: Take the substrate from the seed tray, shape it into a ball, gently toss it 0.5m into the air and catch it. Judge the degree of balling according to the following standards: The ball remains intact and does not break apart: high ball-binding degree; Ball dispersion: Medium ball density; The balls are completely dispersed: low balling density.

[0069] III. Analysis of Test Results for Each Embodiment and Comparative Example The amount of nitrogen-fixing bacteria added to the substrate in Examples 1-3 and Comparative Example 1 was different. The survival rate, average plant height and growth statistics of the obtained Arundo donax domesticated seedlings are shown in Table 1.

[0070] Table 1. Detection data of *Arundinaria buergeriana* seedlings under different nitrogen-fixing bacteria dosages.

[0071] As shown in Table 1, when the amount of nitrogen-fixing bacteria added was 3 parts, the survival rate and growth of the *Arundinaria mirifica* seedlings were significantly improved compared to Comparative Example 1 without the addition of nitrogen-fixing bacteria. A higher amount of nitrogen-fixing bacteria resulted in higher substrate pelleting, indicating that the extracellular polysaccharides secreted by the nitrogen-fixing bacteria can promote substrate pelleting. In Comparative Example 3, no pelleting promoter was added, and the substrate pelleting was significantly lower than in Example 1, indicating that the pelleting promoter can significantly improve the substrate pelleting.

[0072] In Examples 1 and 4-5, the pH of the substrate was different. The survival rate, average plant height and growth statistics of the obtained Arundo donax domesticated seedlings are shown in Table 2.

[0073] Table 2. Detection data of Arundo donax acclimatized seedlings under different pH conditions

[0074] As shown in Table 2, when the substrate pH was adjusted to 7.0 using lime, the survival rate of the domesticated Reed Piper longifolia seedlings was the highest, reaching 95.6%, and the growth was the best, with an average plant height of 27.3 cm. Nitrogen-fixing bacteria are sensitive to substrate pH; both excessive acidity and alkalinity inhibit their activity. A pH of around 7.0 is their optimal pH level.

[0075] The different amounts of Reed charcoal added to the substrate in Examples 1, 6-7 and Comparative Example 1 resulted in different survival rates, average plant heights and growth statistics for the domesticated Reed seedlings. These statistics are shown in Table 3.

[0076] Table 3. Test data of Arundo donax acclimatized seedlings under different Arundo donax charcoal application rates.

[0077] As shown in Table 3, the combined use of reed charcoal and nitrogen-fixing bacteria increased the survival rate of reed seedlings to 97.0% when 5 parts of reed charcoal were added, which was superior to the substrate formulations in Comparative Example 2 without reed charcoal or with 1 and 3 parts of reed charcoal. The main advantage was that the growth was significantly better than in Comparative Example 2, with thicker stems, increased tillering number, and well-developed root system within the substrate bulb.

[0078] It should be noted that this application is not limited to the above-described embodiments. The above embodiments are merely examples, and any embodiments with the same structure and effect as the technical concept within the scope of this application are included in the technical scope of this application. Furthermore, various modifications that can be conceived by those skilled in the art to the embodiments, and other ways of constructing by combining some of the constituent elements of the embodiments, without departing from the spirit of this application, are also included in the scope of this application.

Claims

1. A substrate for the acclimatization of Reed seedlings, characterized in that, The mixture includes humus, perlite, organic fertilizer, ball-forming promoter, nitrogen-fixing bacteria, and reed charcoal, wherein the ball-forming promoter includes KH2PO4, MgSO4·7H2O, and CaSO4·2H2O.

2. The reed seedling acclimatization substrate according to claim 1, characterized in that, By weight, it includes 60 parts humus, 20 parts perlite, 15 parts organic fertilizer, 1-3 parts ball-forming promoter, 2-4 parts nitrogen-fixing bacteria, and 1-5 parts reed charcoal. The mass ratio of KH2PO4, MgSO4·7H2O and CaSO4·2H2O in the ball-forming promoter is 1:1:

1.

3. The reed seedling acclimatization substrate according to claim 1, characterized in that, The reed charcoal is obtained by drying and crushing the reed stems and stalks, followed by high-temperature anaerobic pyrolysis. The high-temperature pyrolysis temperature is 800~900℃, and the time is 6~9s; The specific surface area of ​​the reed charcoal is 205.5~216.5 m². 3 / g, with a fixed carbon content of 68.5%–76.5%.

4. The reed seedling acclimatization substrate according to claim 1, characterized in that, The nitrogen-fixing bacteria is *Azotobacter chrysophylloides*.

5. A highly efficient method for cultivating domesticated seedlings of Phragmites australis, characterized in that, Includes the following steps: After pretreatment and cleaning, the tissue culture seedlings of Reed are used to obtain single tissue culture seedlings. The single tissue culture seedlings were transplanted into a substrate and cultured to obtain acclimatized seedlings. The substrate is the reed seedling acclimatization substrate as described in any one of claims 1 to 4, the pH of the substrate is adjusted to 6.5 to 7.5, and the carbon-nitrogen ratio of the substrate is (40 to 70):

1.

6. The efficient method for cultivating *Phragmites australis* acclimatized seedlings according to claim 5, characterized in that, The pretreatment step includes: keeping the Reed tissue culture bottle containing the Reed tissue culture seedlings unopened and culturing it for 10 days under natural light at 20-30°C.

7. The efficient method for cultivating *Phragmites australis* acclimatized seedlings according to claim 5, characterized in that, The cleaning step includes: cleaning the roots of the pretreated Reed tissue culture seedlings, dividing them into individual plants, cutting the root length to 2-3 cm and the plant height to 6-7 cm, to obtain individual tissue culture seedlings.

8. The efficient method for cultivating *Phragmites australis* acclimatized seedlings according to claim 5, characterized in that, The individual tissue culture seedlings were soaked in 0.1% to 0.2% carbendazim for 15 minutes.

9. The efficient method for cultivating *Phragmites australis* acclimatized seedlings according to claim 1, characterized in that, The cultivation steps include: culturing under light for 10-14 hours at a temperature of 25-30℃ and a substrate humidity of 70% of its maximum water holding capacity, with a light intensity of 5000-10000 lx, followed by culturing in the dark for 10-14 hours.

10. The efficient method for cultivating *Arundinaria lobata* acclimatized seedlings according to claim 1, characterized in that, The pH of the matrix was adjusted to 6.5-7.5 using lime.

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