Cotton endogenous glycometabolism regulating agent and application thereof

Abstract

The invention belongs to the technical field of plant health regulation and control, and discloses a cotton endogenous glycometabolism regulating agent and application thereof. The cotton endogenous glycometabolism regulating agent comprises an invertase inhibition composition, a stress-resistant composition, ozone and water, the invertase inhibition composition is prepared from one or more of tea polyphenol, oligosaccharide, a flavonoid compound, amino ethyoxyl vinyl glycine and EDTA (Ethylene Diamine Tetraacetic Acid) metal chelate salt; the stress-resistant composition comprises one or more of gossypol, salicylic acid, jasmonic acid and methyl ester derivatives thereof, abscisic acid, allyl thiazole, sodium dichloroisocyanurate, potassium silicate, a seaweed extract and a microbial additive. The application comprises the following steps: mixing the invertase inhibition composition and the stress-resistant composition into water to form a mixed solution or suspension; and pumping ozone into the mixed solution or the suspension through a nanocrystallization turbine pump, so that the ozone is dispersed into nano bubbles in the mixed solution or the suspension, and the cotton endogenous glycometabolism regulating agent is obtained.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of plant health regulation, and particularly relates to a cotton endogenous sugar metabolism regulator and application thereof. BACKGROUND

[0002] At present, the sugar content of cotton in Xinjiang and other regions is relatively high, and there is a problem of increased difficulty in seed cotton separation, thereby affecting the working efficiency of ginning equipment. According to existing research, the main reason for the excessively high endogenous sugar content of cotton is that the photosynthesis is strong during the day, and a large amount of sugar is synthesized, and the enzyme activity is limited at low temperature at night, so that the sugar cannot be converted into cellulose in time. In addition, pest damage also leads to the accumulation of endogenous sugars in cotton, for example: aphid damage hinders the transportation of sugars by damaging the vascular bundle, and the digestive juice secreted interferes with sugar metabolism; the damage of thrips induces the defense response of cotton, leading to the accumulation of sugars at the invaded site; the damage of whitefly induces the stress response of cotton, and more secondary metabolites, including some sugar substances, are synthesized to enhance the defense ability of the body, and these extra synthesized sugars will lead to the increase of the endogenous sugar content of cotton.

[0003] Therefore, it is necessary to provide a cotton endogenous sugar metabolism regulator and an application method for fundamentally reducing the endogenous sugar content of cotton by inhibiting glucose conversion in the present application. SUMMARY

[0004] In view of this, in order to solve the problems raised in the background art, the purpose of the present application is to provide a cotton endogenous sugar metabolism regulator and application thereof.

[0005] In order to achieve the above-mentioned purpose, the present application provides the following technical scheme: a cotton endogenous sugar metabolism regulator, comprising a transmembrane enzyme inhibiting composition, a stress resistance composition, ozone and water.

[0006] Ozone has strong oxidizing property, which causes cell membrane damage, thereby leading to the outbreak of intracellular ROS, mitochondrial damage to generate superoxide anion (O2 - ) and conversion to H2O2 and OH - . The -SH in the active center of transmembrane enzyme is oxidized to sulfonic group (-SO3H) or disulfide bond (-S-S-), thereby causing the rigidity of transmembrane enzyme conformation and the inability to bind substrate (sucrose), so as to achieve the effect of inhibiting the synthesis of endogenous monosaccharide of cotton.

[0007] The transmembrane enzyme inhibiting composition comprises one or more of tea polyphenols, oligosaccharides, flavonoids, aminoethoxyvinyl glycine and EDTA metal chelate salt.

[0008] Tea polyphenols: have various biological activities. Studies have found that tea polyphenols have a certain inhibitory effect on invertase. It may bind to the active site of invertase or change the spatial conformation of the enzyme, thereby affecting the binding ability of the enzyme and the substrate, and then inhibiting the activity of invertase. For example, in some fruit preservation studies, the use of tea polyphenols can inhibit the activity of invertase in fruits, reduce the hydrolysis of sucrose, and help maintain the sweetness and quality of fruits.

[0009] Oligosaccharides (such as chitosan or amino oligosaccharides): have various biological activities. In plants, amino oligosaccharides can induce plants to produce disease resistance, etc. Studies have shown that amino oligosaccharides may indirectly affect the activity of invertase by regulating hormone levels and metabolic pathways in plants. In addition, chitosan can activate the reactive oxygen species (ROS) signaling pathway in cotton cells, induce lignin deposition and callose synthesis, and enhance the mechanical strength of the cell wall; at the same time, it can inhibit the growth of pathogenic fungi (such as Verticillium dahliae). Oligosaccharides (such as oligogalacturonide) as elicitors can trigger the pattern recognition receptor (PRR) of cotton, activate the MAPK signaling pathway, and induce the expression of defense genes (such as PAL, CHI) and the accumulation of secondary metabolites (such as flavonoids).

[0010] Flavonoids (such as quercetin-3-rutinoside): as a flavonoid, it has a certain inhibitory effect on invertase. Its inhibitory mechanism may be similar to that of tea polyphenols, by interacting with enzyme molecules, affecting the active site or spatial structure of the enzyme, thereby reducing the catalytic efficiency of invertase.

[0011] Aminoethoxyvinylglycine (AVG): its main function is to inhibit the synthesis of ethylene in plants. Ethylene, as a plant hormone, is involved in regulating plant growth and development, fruit ripening, and other processes. During fruit ripening, ethylene promotes the activity of invertase and accelerates the hydrolysis of sucrose. AVG, by inhibiting the synthesis of ethylene, indirectly reduces the activity of invertase and the hydrolysis of sucrose, thereby delaying the ripening process of fruits, and has certain applications in fruit preservation, etc.

[0012] EDTA metal chelate salts (such as EDTA dipotassium, EDTA disodium): their biological effects mainly occur outside cells, chelating extracellular metal ions Ca 2+ , Mg 2+ , etc., inhibiting metal ion-dependent extracellular enzymes. Chelating metal ions on the surface of cell membranes affects cell adhesion, signal transduction, or ion channel function.

[0013] The stress-resistant composition includes one or more of gossypol, salicylic acid, jasmonic acid and its methyl ester derivatives, abscisic acid, allyl thiazole, sodium dichloroisocyanurate, potassium silicate, seaweed extract, and microbial additives.

[0014] Salicylic acid (SA) induces systemic acquired resistance (SAR) in cotton, activates the expression of pathogenesis-related protein (PR protein) genes, and enhances the resistance to fungal diseases such as Verticillium wilt and Fusarium wilt.

[0015] Jasmonic acid (JA) and its methyl ester derivative (MeJA) induce induced systemic resistance (ISR) in cotton, promote the synthesis of plant protectants (such as gossypol) and cell wall thickening, and enhance the defense ability against pests such as cotton bollworm and red spider mite.

[0016] Abscisic acid (ABA) regulates stomatal closure, reduces water loss, and enhances the tolerance of cotton to abiotic stresses such as drought and salinity; and participates in the expression regulation of stress-related genes (such as drought-resistant gene Rab18).

[0017] Gossypol has antifeedant and toxic effects on pests such as cotton bollworm and aphids; and can inhibit the growth and reproduction of pathogenic fungi (such as Fusarium oxysporum), which is an important component of natural resistance in cotton.

[0018] Probenazole induces the production of disease-resistant enzymes (such as peroxidase and polyphenol oxidase) in cotton, enhances the resistance of cell wall structure, and is mainly used for the prevention and control of fungal diseases at the seedling stage.

[0019] Sodium dichloroisocyanurate (SDIC) releases hypochlorous acid and active oxygen, damages the cell structure of pathogenic fungi, and can also stimulate cotton to produce stress resistance, which is commonly used for soil disinfection and seed treatment.

[0020] Silicon in potassium silicate is absorbed by cotton in the form of soluble silicate, which is deposited in the cell wall to form a "silicon layer" and enhance the mechanical resistance; and can also activate the antioxidant system to alleviate salt stress and high temperature damage.

[0021] Seaweed extract (seaweed polysaccharide, amino acid, trace element) promotes root development, enhances the tolerance of cotton to drought and salinity; and can also induce the synthesis of osmotic regulators (such as proline) to maintain the balance of cell osmotic pressure.

[0022] Microbial additives at least include one or more of Bacillus subtilis, Metarrhizium anisopliae, Beauveria bassiana, Trichoderma, and arbuscular mycorrhizal fungi.

[0023] Biocontrol bacteria such as Bacillus subtilis enhance the resistance to Verticillium wilt and Fusarium wilt by competing for sites, secreting antibacterial substances (such as lipopeptide compounds), and inducing cotton to produce ISR (such as activating the JA signaling pathway).

[0024] Biocontrol fungi such as Metarrhizium anisopliae, Beauveria bassiana, and Trichoderma secrete cellulase and chitinase to degrade the cell walls of pathogenic fungi, and induce cotton to synthesize plant protectants and antioxidant enzymes, thereby improving the tolerance to Verticillium wilt.

[0025] Arbuscular mycorrhizal fungi form a symbiotic relationship with cotton roots, improve nutrient absorption, and indirectly enhance the plant's resistance to adversity (such as nitrogen deficiency, drought).

[0026] As a general inventive concept, the present application also provides the following technical solutions:

[0027] The application of the cotton endogenous sugar metabolism regulator disclosed above in reducing the endogenous monosaccharide content of cotton. Specifically: the invertase inhibiting composition in the cotton endogenous sugar metabolism regulator inhibits cotton invertase activity; the stress resistance inducing composition in the cotton endogenous sugar metabolism regulator induces cotton pest resistance.

[0028] The method of the application comprises the following steps:

[0029] S1. Mixing the invertase inhibiting composition and the stress resistance inducing composition in water to form a mixed solution or suspension;

[0030] S2. Pumping ozone into the mixed solution or suspension through a nanometerizing turbine pump, so that the ozone is dispersed into nanometer bubbles in the mixed solution or suspension, to obtain a gas-liquid mixed cotton endogenous sugar metabolism regulator;

[0031] S3. Applying the cotton endogenous sugar metabolism regulator during cotton planting and growth.

[0032] Preferably, soil drip irrigation and / or foliar spraying are used when applying the cotton endogenous sugar metabolism regulator, the amount of the invertase inhibiting composition is 1g-500g per mu, the amount of the stress resistance inducing composition is 1g-5000g per mu, and the amount of the ozone is 100g / h-1000g / h.

[0033] Compared with the prior art, the present application has the following beneficial effects:

[0034] The cotton endogenous sugar metabolism regulator provided by the present application comprises an invertase inhibiting composition, a stress resistance inducing composition, ozone, etc., wherein the invertase inhibiting composition reduces the endogenous monosaccharide content of cotton by inhibiting invertase activity, and the stress resistance inducing composition reduces the endogenous monosaccharide synthesized due to pest stress response by inducing pest resistance, thereby fundamentally solving the problem of high sugar content in cotton in a multi-party synergistic manner, and the sources of the various compounds are sufficient, the cost is low, the environment is not polluted, and the environmental protection requirements are met. DETAILED DESCRIPTION

[0035] For a further understanding of the present application, the application will be described in detail with reference to the embodiments. The structures, proportions, sizes, etc. shown in the embodiments are merely used to illustrate the content disclosed in the specification, for the understanding and reading of those skilled in the art, and are not used to limit the limiting conditions that the present application can be implemented, so they do not have substantial technical significance. Any modification of the structure, change of the proportion relationship or adjustment of the size, without affecting the effect and purpose that the present application can produce, should still fall within the scope of the technical content disclosed by the present application. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and the like in the specification are merely for the purpose of clear understanding of the description, and are not used to limit the scope of implementation, and the change or adjustment of the relative relationship, without substantially changing the technical content, is also regarded as the scope of implementation of the present application. It should be noted that the terms "first", "second" and the like in the specification and claims of the present application are used to distinguish similar objects, and do not necessarily describe a specific order or sequence. It should be understood that such data can be interchanged under appropriate circumstances, so as to describe the embodiments of the present application.

[0036] The present embodiment provides a cotton endogenous sugar metabolism regulator, including invertase inhibitor composition, stress resistance composition, ozone and water.

[0037] (1) About invertase inhibitor composition

[0038] Specifically including one or more of tea polyphenols, oligosaccharides, flavonoids, aminoethoxyvinyl glycine, and EDTA metal chelate salt.

[0039] Tea polyphenols: have a variety of biological activities, research has found that tea polyphenols have a certain inhibitory effect on invertase. It may bind to the active site of invertase, or change the spatial conformation of the enzyme, thereby affecting the binding ability of the enzyme and the substrate, and then inhibiting the activity of invertase. For example, in some fruit preservation studies, using tea polyphenol treatment can inhibit the activity of invertase in fruit, reduce the hydrolysis of sucrose, and help maintain the sweetness and quality of the fruit.

[0040] For this purpose, the present embodiment also provides the following examples of tea polyphenol inhibiting invertase activity:

[0041] Example 1. During the postharvest storage of kiwifruit, the increase in acid invertase activity will cause the decomposition of sucrose into glucose and fructose, affecting the firmness and flavor of the fruit. Soak the fruit in a tea polyphenol solution (200 mg / L) for 2 h, and measure the invertase activity after 15 days of storage. Compared with the control group (without soaking treatment with tea polyphenol solution), the invertase activity of the treatment group is reduced by 35%, and the decrease in fruit firmness is slowed down (delaying softening).

[0042] Example 2. In fruit juice processing, invertase of wild yeast (e.g., Saccharomyces cerevisiae) can decompose sucrose to produce alcohol, causing the juice to deteriorate. Apple juice is added with tea polyphenol (50 mg / L) and stored in the dark. After 7 days, invertase activity and alcohol content are detected. The results show that the invertase activity is only 38% of the control group (apple juice without tea polyphenol), the alcohol content decreases from 0.8% vol to 0.2% vol, and the total phenol content of the juice increases by 12% (enhancing antioxidant properties).

[0043] Oligosaccharides (e.g., chitosan or amino-oligosaccharides): have various biological activities. In plants, amino-oligosaccharides can induce plants to produce disease resistance, etc. Studies have shown that amino-oligosaccharides may indirectly affect the activity of invertase by regulating hormone levels and metabolic pathways in plants.

[0044] For this purpose, the present embodiment also provides the following examples of amino-oligosaccharides inhibiting invertase activity:

[0045] Example 1. Spray 1% amino-oligosaccharide solution on rice seedlings to induce the secretion of ethylene precursor ACC from rice root system. Compared with the control group (without spraying amino-oligosaccharide solution), the leaf ethylene ET content increases by 60%, the ABA content decreases by 40%, ethylene ET promotes the burst of reactive oxygen species (ROS) and cell wall strengthening (such as lignin synthesis), inhibits sucrose invertase, and enhances disease resistance.

[0046] In addition, chitosan can also activate the active oxygen (ROS) signaling pathway in cotton cells, induce lignin deposition and callose synthesis, and enhance the mechanical strength of the cell wall; at the same time, it can inhibit the growth of pathogenic fungal hyphae (such as Verticillium wilt). Oligosaccharides (such as oligogalacturonide) as elicitors can trigger the pattern recognition receptor (PRR) of cotton, activate the MAPK signaling pathway, induce the expression of defense genes (such as PAL, CHI), and accumulate secondary metabolites (such as flavonoids).

[0047] Flavonoids (e.g., quercetin-3-rutinose): as a flavonoid, it has a certain inhibitory effect on invertase. Its inhibition mechanism may be similar to that of tea polyphenols, by interacting with enzyme molecules, affecting the active site or spatial structure of the enzyme, thereby reducing the catalytic efficiency of invertase.

[0048] For this purpose, the present embodiment also provides the following examples of quercetin-3-rutinose (hereinafter referred to as quercetin) inhibiting invertase activity:

[0049] Example 1. Quercetin regulates sucrose metabolism in Arabidopsis thaliana: Arabidopsis roots were treated with 100 μΜ quercetin solution, and the control group was the medium without quercetin. The results showed that the invertase activity was significantly reduced, and the acid invertase AI activity decreased by 62% and the neutral invertase NI activity decreased by 48% compared with the control group. In addition, within the range of 50-200 μΜ, the higher the quercetin concentration, the stronger the inhibition of invertase activity.

[0050] Example 2. Inhibitory effect of quercetin on midgut invertase in Helicoverpa armigera: 0.1% quercetin was added to the feed, and the larval intake, enzyme activity, and growth rate were detected, and the control group was the medium without quercetin. The results showed that the midgut invertase activity decreased by 45%, the larval body weight decreased by 32% compared with the control, and the development period was prolonged by 5 days. As a plant secondary metabolite, quercetin can enhance the resistance of plants to insects by inhibiting the activity of insect digestive enzymes.

[0051] Aminoethoxyvinylglycine (AVG): Its main function is to inhibit the synthesis of ethylene in plants. Ethylene, as a plant hormone, is involved in regulating plant growth and development and fruit ripening. During fruit ripening, ethylene promotes invertase activity and accelerates sucrose hydrolysis. AVG indirectly reduces invertase activity and reduces sucrose hydrolysis by inhibiting ethylene synthesis, thereby delaying the ripening process of fruits and having certain applications in fruit preservation.

[0052] Therefore, the present embodiment also provides the following examples of aminoethoxyvinylglycine inhibiting invertase activity:

[0053] Example 1. Inhibition of invertase in Arabidopsis roots by AVG: Arabidopsis seedlings were treated by soaking the roots in a medium containing 50 μΜ AVG for 48 h, and the control group was the medium without AVG. The results showed that the activity of cell wall-bound invertase (CWIN) decreased by 39%, the sucrose unloading in the roots was blocked, and the sucrose transport to the aerial part was reduced by 25%.

[0054] Example 2. Inhibition of invertase in postharvest grapes by AVG: Giantbunch grape fruits were treated with 20 mg / L AVG fumigation after harvest, and the invertase activity and fruit quality during storage were detected, and the control group was the fruit without AVG fumigation treatment. On the 30th day of storage, the acid invertase AI activity of the treated group was 41% lower than that of the control group, and the neutral invertase NI activity was 35% lower, and the sucrose decomposition rate was significantly reduced.

[0055] Example 3. Inhibition of invertases during tomato fruit ripening by AVG: Tomato fruits were sprayed with 100 μM AVG solution at green-ripe stage, and the activities of invertases and the expression of related genes were detected at red-ripe stage. The control group was not sprayed with AVG solution. The results showed that the activities of acid invertase AI and neutral invertase NI decreased by 53% and 48%, respectively, and the mRNA levels of AI and NI decreased by 58% and 51%, respectively.

[0056] EDTA metal chelate salt (e.g. EDTA dipotassium, EDTA disodium): The main biological effect occurs outside the cell, chelating extracellular metal ions Ca 2+ , Mg 2+ , etc., inhibiting metal ion-dependent extracellular enzymes. Chelating metal ions on the surface of the cell membrane affects cell adhesion, signal transduction or ion channel function.

[0057] (2) About the stress resistance composition

[0058] Specifically including one or more of gossypol, salicylic acid, jasmonic acid and its methyl ester derivatives, abscisic acid, allyl thiadiazole, sodium dichloroisocyanurate, potassium silicate, seaweed extract, and microbial additives.

[0059] Salicylic acid (SA) induces cotton to produce systemic acquired resistance (SAR), activates the expression of pathogenesis-related protein (PR protein) genes, and enhances the resistance to fungal diseases such as verticillium wilt and fusarium wilt.

[0060] Jasmonic acid (JA) and its methyl ester derivatives (MeJA) induce cotton to produce induced systemic resistance (ISR), promote the synthesis of plantin (such as gossypol) and thickening of the cell wall, and enhance the defense ability against pests such as cotton bollworm and red spider.

[0061] Abscisic acid (ABA) regulates stomatal closure, reduces water loss, and enhances the tolerance of cotton to abiotic stresses such as drought and salinity; and participates in the expression regulation of stress-related genes (such as drought-resistant gene Rab18).

[0062] Gossypol has antifeedant and toxic effects on pests such as cotton bollworm and aphids; and can inhibit the growth and reproduction of pathogenic bacteria (such as fusarium), which is an important component of natural resistance of cotton.

[0063] Allyl thiadiazole (Probenazole) induces cotton to produce disease-resistant enzymes (such as peroxidase and polyphenol oxidase), enhances the resistance of cell wall structure, and is mainly used for preventing and treating fungal diseases at seedling stage.

[0064] Sodium dichloroisocyanurate (SDIC) releases hypochlorous acid and active oxygen, damages the cell structure of pathogenic bacteria, and can also stimulate cotton to produce stress resistance, and is commonly used for soil disinfection and seed treatment.

[0065] Silicon in potassium silicate is absorbed by cotton in the form of soluble silicate, deposited in the cell wall to form a "silicon layer" and enhance mechanical resistance; at the same time, it can activate the antioxidant system to alleviate salt stress and high temperature damage.

[0066] Seaweed extract (seaweed polysaccharide, amino acid, trace element) promotes root development and enhances the tolerance of cotton to drought, salinity and alkalinity; at the same time, it can induce the synthesis of osmotic adjustment substances (such as proline) to maintain the balance of cell osmotic pressure.

[0067] The microbial additive at least includes one or more of Bacillus subtilis, Metarrhizium anisopliae, Beauveria bassiana, Trichoderma, and arbuscular mycorrhiza.

[0068] Biocontrol bacteria such as Bacillus subtilis can enhance the resistance to fusarium wilt and rhizoctonia damping-off by competing for sites, secreting antibacterial substances (such as lipopeptide compounds), and inducing cotton to produce ISR (such as activating the JA signal pathway).

[0069] Biocontrol fungi such as Metarrhizium anisopliae, Beauveria bassiana, and Trichoderma can secrete cellulase and chitinase to degrade the cell wall of pathogenic fungi, and at the same time induce cotton to synthesize plant phenolics and antioxidant enzymes to improve the tolerance to verticillium wilt.

[0070] Arbuscular mycorrhiza forms a symbiotic relationship with cotton roots, improves nutrient absorption, and indirectly enhances the resistance of plants to adversity (such as nitrogen deficiency and drought).

[0071] (3) About ozone

[0072] Ozone has strong oxidizing properties, causing cell membrane damage, leading to the outbreak of intracellular ROS, mitochondrial damage generating superoxide anion (O2 - ) and converting into H2O2 and OH - . The -SH in the active center of the enzyme is oxidized to sulfonic acid group (-SO3H) or disulfide bond (-S-S-), which further causes the enzyme to become rigid in conformation and unable to bind to substrate (sucrose), thereby inhibiting the synthesis of endogenous monosaccharides in cotton.

[0073] Therefore, the embodiment also provides the following examples of ozone inhibiting invertase activity:

[0074] Example 1. Ozone (0.3 ppm) treatment of Arabidopsis thaliana leaves for 4 h can increase intracellular H2O2 levels by 3 times, while acid invertase activity decreases by 50%. Pretreatment with N-acetyl cysteine (NAC, a ROS scavenger) can completely reverse the inhibition of enzyme activity. Western blotting shows that the level of thiol oxidation modification of invertase is significantly increased after ozone treatment.

[0075] Example 2. When S. cerevisiae was treated with ozone (0.5 mg / L), the first observation was the increase of MDA, a lipid peroxidation product of cell membrane, followed by a 70% decrease of invertase activity within 30 min. Electron microscopy showed holes in the cell membrane, and enzyme molecules leaked out through the holes and were further degraded by ROS.

[0076] The one or more compounds described above are synergistic with ozone, and the application also provides the use of the cotton endogenous sugar metabolism regulator in reducing the content of endogenous monosaccharides in cotton, and the specific application method comprises the following steps:

[0077] S1. Mix the invertase inhibitor composition and the stress resistance composition in water to form a mixed solution or suspension;

[0078] S2. Pump ozone into the mixed solution or suspension through a nanomization turbine pump, so that the ozone is dispersed into nanobubbles in the mixed solution or suspension, to obtain a gas-liquid mixed cotton endogenous sugar metabolism regulator;

[0079] S3. Apply the cotton endogenous sugar metabolism regulator in the process of cotton planting and growth by soil drip irrigation and / or foliar spraying, and control the use amount of the invertase inhibitor composition to be 1-500 g / acre, the use amount of the stress resistance composition to be 1-5000 g / acre, and the use amount of the ozone to be 100-1000 g / h.

[0080] Specifically, taking soil drip irrigation as an example:

[0081] Drip irrigation operation I

[0082] First, mix tea polyphenols (100 g / acre) in water to form a mixed solution; an ozone generator generates ozone at a rate of 500 g / h, and then pumps the ozone into the mixed solution through a nanomization turbine pump; drip the cotton endogenous sugar metabolism regulator into the soil through the drip irrigation system, and set the water dripping amount of the drip irrigation system to be 5 m 3 / acre.

[0083] Then, mix Bacillus subtilis (100 g / acre) in water to form a mixed solution; an ozone generator generates ozone at a rate of 500 g / h, and then pumps the ozone into the mixed solution through a nanomization turbine pump; drip the cotton endogenous sugar metabolism regulator into the soil through the drip irrigation system, and set the water dripping amount of the drip irrigation system to be 5 m 3 / acre.

[0084] Drip irrigation operation II

[0085] First, tea polyphenols (100 g / acre) and bacillus subtilis (100 g / acre) are mixed in water to form a mixed solution or suspension; an ozone generator generates ozone at a rate of 500 g / h, which is then pumped into the mixed solution or suspension by a nanometerization turbine pump; finally, the cotton endogenous sugar metabolism regulator is drip irrigated into the soil through a drip irrigation system, and the drip irrigation system is set to drip 5 m 3 / acre of water.

[0086] Specifically, taking foliar spraying as an example:

[0087] High-sugar cotton fields with sugar content of 5% to 7%: spray 0.5% chitosan + 0.3% potassium dihydrogen phosphate, combined with nitrogen control and potassium increase, low cost and high safety.

[0088] High-sugar cotton fields with sugar content of >7%: aminoethoxy ethenyl glycine (80 mg / L) is compounded with chemical control agent mepiquat chloride (3 g / acre), and is sprayed in the flower and boll stage to rapidly reduce sugar accumulation and promote leaf abscission and ripening in the later stage.

[0089] Note that the above are only preferred embodiments of the present application and the technical principles applied. Those skilled in the art will understand that the present application is not limited to the specific embodiments described herein, and those skilled in the art can make various obvious changes, readjustments and substitutions without departing from the scope of the present application. Therefore, although the present application has been described in more detail through the above embodiments, the present application is not limited to the above embodiments, and can include more other equivalent embodiments without departing from the concept of the present application, and the scope of the present application is determined by the scope of the appended claims.

Claims

1. A cotton endogenous sugar metabolism regulator, characterized in that: Includes an enzyme-inhibiting composition, an anti-stress composition, ozone, and water; The invertase inhibitory composition comprises one or more of the following: tea polyphenols, oligosaccharides, flavonoids, aminoethoxyvinylglycine, and EDTA metal chelate salts. The stress-resistant composition includes one or more of the following: gossypol, salicylic acid, jasmonic acid and its methyl ester derivatives, abscisic acid, allylthiazole, sodium dichloroisocyanurate, potassium silicate, seaweed extract, and microbial additives.

2. The cotton endogenous sugar metabolism regulator according to claim 1, characterized in that: The oligosaccharides include chitosan and amino oligosaccharides.

3. The cotton endogenous sugar metabolism regulator according to claim 1, characterized in that: The flavonoids include quercetin-3-rutin.

4. The cotton endogenous sugar metabolism regulator according to claim 1, characterized in that: The EDTA metal chelate salts include dipotassium EDTA and disodium EDTA.

5. The cotton endogenous sugar metabolism regulator according to claim 1, characterized in that: The microbial additives include at least one or more of Bacillus subtilis, Metarhizium anisopliae, Beauveria bassiana, Trichoderma, and Arbuscular mycorrhizal fungi.

6. The application of the cotton endogenous sugar metabolism regulator as described in any one of claims 1-5 in reducing the endogenous monosaccharide content of cotton.

7. The application according to claim 6, characterized in that: The invertase-inhibiting composition in the cotton endogenous sugar metabolism regulator inhibits cotton invertase activity; the stress-resistant composition in the cotton endogenous sugar metabolism regulator induces cotton pest resistance.

8. The application according to claim 6, characterized in that, Includes the following steps: S1. Mix the invertase inhibitory composition and the anti-stress composition in water to form a mixed solution or suspension; S2. Ozone is pumped into the mixed solution or suspension using a nano-turbine pump, so that the ozone is dispersed into nanobubbles in the mixed solution or suspension, thereby obtaining a gas-liquid mixed cotton endogenous sugar metabolism regulator. S3. Apply the cotton endogenous sugar metabolism regulator during cotton planting and growth.

9. The application according to claim 8, characterized in that: The application of the cotton endogenous sugar metabolism regulator includes soil drip irrigation and / or foliar spraying.

10. The application according to claim 8, characterized in that: The dosage of the invertase inhibitory composition is 1g to 500g / mu, the dosage of the stress-resistant composition is 1g to 5000g / mu, and the dosage of ozone is 100g / h to 1000g / h.