Herbal composite sesame oil with effects of clearing heat and protecting stomach and preparation method thereof

Abstract

The invention discloses herbal composite sesame oil with effects of clearing heat and protecting stomach and a preparation method thereof, and the herbal composite sesame oil comprises the following components in parts by mass: 500-600 parts of soybean oil, 300-400 parts of sesame oil, 12-18 parts of mesona chinensis, 8-12 parts of plumeria rubra, 18-22 parts of microcos paniculata, 12-18 parts of chrysanthemum, 12-18 parts of honeysuckle, 22-28 parts of selfheal and 18-22 parts of liquorice. According to the invention, seven herbs including mesona chinensis, plumeria rubra, microcos paniculata, chrysanthemum, honeysuckle, selfheal and liquorice are precisely combined, and a synergistic interaction effect is realized in a grease medium. Especially, the combination of microcos paniculata, prunella vulgaris and plumeria rubra proves that the gastric mucosa protection rate is increased by 38.7% in animal experiments, which indicates that the product has a remarkable effect on relieving gastrointestinal discomfort caused by spicy diet.

Description

Technical Field

[0001] This invention belongs to the field of functional edible oil technology, specifically relating to a herbal compound sesame oil with heat-clearing and stomach-protecting effects and its preparation method. It is a hot pot seasoning oil that integrates traditional Chinese medicine theory and solves the problems of internal heat and gastrointestinal mucosal damage caused by spicy food through specific herbal compatibility and preparation process. Background Technology

[0002] Sesame oil is typically extracted from sesame seeds through pressing or stone milling with water. Traditionally, hot pressing is the most common method, as it imparts the unique aroma and flavor to sesame oil. Cold pressing, performed without heating, retains more of the natural nutrients. Modern processes also include solvent extraction to increase oil yield. Sesame oil is rich in monounsaturated and polyunsaturated fatty acids, especially linoleic and oleic acids, as well as micronutrients such as vitamin E (an antioxidant) and phytosterols. It also contains sesamol and other lignans. The unsaturated fatty acids in sesame oil help lower bad cholesterol levels and protect the heart; certain components have anti-inflammatory effects and may help alleviate inflammatory diseases; vitamin E and other antioxidants help combat free radical damage and slow down the aging process.

[0003] Although sesame oil itself contains many beneficial substances, current improvements to sesame oil mainly focus on flavor enhancement, with little attention paid to improving its health benefits. Researchers are currently dedicated to adding herbal extracts to sesame oil to further improve its overall efficacy. For example, Chinese patent CN105901170A discloses a stomach-strengthening and antioxidant sesame oil made from the following ingredients by weight: 80-120 parts sesame oil, 10-20 parts apple, 5-15 parts kiwi, 3-10 parts grape, 3-5 parts fennel, 3-5 parts Sichuan pepper, 6-8 parts cinnamon, and 0.16-0.24 parts iodized salt. This health-promoting sesame oil is rich in nutrients, including beneficial unsaturated fatty acids, various vitamins, and trace elements. It is suitable for long-term consumption and is said to have anti-aging effects, enhance memory, and prevent cardiovascular diseases. It also has stomach-strengthening and appetite-enhancing effects. While the addition of the aforementioned fruits, as well as Sichuan peppercorns and cinnamon, can impart certain health benefits to sesame oil, the direct mixing of the fruit juice and compound oil during the preparation process affects their compatibility. This only allows them to maintain uniformity for a short period, resulting in a shorter actual product shelf life. Furthermore, the high-temperature heating process can have adverse effects on the components of the added substances. Chinese patent CN109007067A discloses a method for preparing stomach-nourishing sesame oil. The method involves pulverizing and mixing Atractylodes macrocephala, Euryale ferox, Astragalus membranaceus, Nelumbo nucifera, Dioscorea opposita, and Polygonatum sibiricum separately to obtain a herbal mixture. Water and an excipient are added to the herbal mixture, and the mixture is decocted, filtered, and the liquid and residue are obtained. Washed sesame seeds are soaked in the herbal liquid, filtered, and dried. The dried sesame seeds and residue are then roasted together to obtain sesame seed cakes. The sesame seed cakes are pressed in an oil press, and the oil and residue are separated to obtain crude oil. The crude oil is then frozen, crystallized, filtered to remove impurities, and kept warm to allow precipitation, resulting in stomach-nourishing sesame oil. This sesame oil has a strong aroma, unique flavor, and health benefits such as strengthening the spleen and stomach. Although this method involves soaking in the herbal liquid, this method still has issues with incomplete soaking and cross-compatibility between the herbal liquid and sesame seeds.

[0004] Therefore, there are still the following problems with introducing herbs into sesame oil to enhance its health benefits:

[0005] (1) The synergistic effect of herbal plants has not been fully developed, resulting in poor efficacy;

[0006] (2) It cannot resolve the contradiction between high temperature sensitization and low temperature precipitation. Herbal plants and sesame oil have poor compatibility. High temperature refining can also easily lead to the degradation and failure of active ingredients.

[0007] (3) The functional substances in herbal plants cannot be stably present in sesame oil. Summary of the Invention

[0008] The purpose of this invention is to provide a herbal compound sesame oil with heat-clearing and stomach-protecting effects and its preparation method, so as to solve the problems that the herbal composition added to the above-mentioned sesame oil has poor efficacy and that the effective substances in the herbal composition cannot exist stably in the sesame oil.

[0009] To achieve the above objectives, the first aspect of the present invention provides a herbal compound sesame oil with heat-clearing and stomach-protecting effects. The herbal compound sesame oil comprises, by weight, 500-600 parts soybean oil, 300-400 parts sesame oil, 12-18 parts mesona chinensis, 8-12 parts frangipani, 18-22 parts buza leaves, 12-18 parts chrysanthemum, 12-18 parts honeysuckle, 22-28 parts prunella vulgaris, and 18-22 parts licorice.

[0010] To address the issue of poor synergistic effects of sesame oil, this invention designs a herbal composition to be added to sesame oil, including seven herbs: mesona chinensis, frangipani, buza leaves, chrysanthemum, honeysuckle, prunella vulgaris, and licorice. Among them, this invention is the first to use buza leaves, prunella vulgaris, and frangipani in an oil medium, which has a synergistic effect and has been experimentally verified to protect the gastric mucosa.

[0011] Preferably, the herbal compound sesame oil, by weight, comprises 550 parts soybean oil, 350 parts sesame oil, 15 parts mesona chinensis, 10 parts frangipani, 20 parts buza leaves, 15 parts chrysanthemum, 15 parts honeysuckle, 25 parts prunella vulgaris, and 20 parts licorice.

[0012] The second aspect of this invention provides a method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects, comprising the following steps:

[0013] (1) Herb pretreatment

[0014] After washing and air-drying the herbs such as mesona chinensis, frangipani, basil, chrysanthemum, honeysuckle, prunella vulgaris, and licorice, they are pulverized to obtain herbal powder.

[0015] (2) Gradient simmering

[0016] Soybean oil is heated and then herbal powder is added. The mixture is then cooled and stirred at a constant temperature. Finally, heating is stopped and the mixture is allowed to cool naturally before being filtered to obtain the medicinal oil. The heating temperature of the soybean oil, the temperature of the constant temperature stirring, and the temperature of the natural cooling decrease in that order.

[0017] (3) Compound emulsification

[0018] After further cooling the medicinal oil, add sesame oil and homogenize to obtain herbal compound sesame oil.

[0019] (4) Aseptic filling

[0020] The herbal compound sesame oil was nitrogen-filled and sealed in a brown glass bottle.

[0021] Preferably, in step (1), the material is pulverized to 20-40 mesh.

[0022] Preferably, in step (2), the heating temperature of soybean oil, the temperature of constant temperature stirring, and the natural cooling temperature are 140-160℃, 110-130℃, and below 60℃, respectively.

[0023] This invention also limits the temperature used in the preparation method and designs a three-stage temperature control process, so that the heating temperature of soybean oil, the constant temperature stirring temperature and the natural cooling temperature decrease sequentially, from 140-160℃ → 110-130℃ → below 60℃, which solves the contradiction between high temperature sensitization and low temperature precipitation, and the effective ingredients in the herbal composition can be retained in sesame oil to the greatest extent.

[0024] Preferably, in step (2), filtration is performed using a 200-mesh filter cloth.

[0025] Preferably, in step (3), the temperature is further reduced to 35-45°C.

[0026] Preferably, in step (3), the homogenization speed is 1000-3000 rpm and the time is 1-10 min.

[0027] Preferably, in step (3), the retention rate of glycyrrhizin, chlorogenic acid, and luteolin in the herbal compound sesame oil is >92%.

[0028] The active ingredients of the herbal composition in this invention can not only be retained in sesame oil, but also exist stably therein. Accelerated tests show that no solid substances were precipitated after 3 months.

[0029] Preferably, the benzo[a]pyrene content in the herbal compound sesame oil is ≤0.8μg / kg.

[0030] The third aspect of this invention provides the application of herbal compound sesame oil with heat-clearing and stomach-protecting effects in the preparation of functional foods that alleviate gastrointestinal stress caused by spicy diets.

[0031] The fourth aspect of this invention provides the application of herbal compound sesame oil with heat-clearing and stomach-protecting effects in the preparation of functional foods that help protect the gastric mucosa.

[0032] Therefore, the present invention provides a herbal compound sesame oil with heat-clearing and stomach-protecting effects using the above-mentioned structure, and its preparation method, which has the following beneficial effects:

[0033] (1) This invention achieves a synergistic effect by precisely combining seven herbs: Mesona chinensis, Plumeria rubra, Bruguiera gymnorhiza, Chrysanthemum morifolium, Lonicera japonica, Prunella vulgaris, and Glycyrrhiza uralensis, and in an oil-based medium. In particular, the combination of Bruguiera gymnorhiza, Prunella vulgaris, and Plumeria rubra has been shown in animal experiments to increase the gastric mucosa protection rate by 38.7%, indicating that this product has a significant effect on relieving gastrointestinal discomfort caused by spicy food.

[0034] (2) The present invention adopts a three-stage temperature control process, which can destroy the plant cell wall to release the active ingredients, and avoid the degradation of the active ingredients caused by high temperature and the precipitation problem at low temperature, thus ensuring the maximum retention and stable coexistence of the active ingredients (HPLC detection retention rate > 92%).

[0035] (3) This invention solves the problem of poor compatibility between traditional herbal water extracts and oils. Through a specific temperature control process, the extraction rate of active ingredients such as chlorogenic acid reaches 82.4%, which is much higher than that of traditional water extraction (56.1%), and the stability in the final product is also guaranteed.

[0036] (4) Experiments have shown that the herbal compound sesame oil of this invention can significantly reduce the serum IL-6 inflammatory factor level after eating hot pot (p<0.01), reduce the incidence of oral ulcers (down 76.5%), and reduce the heartburn score to 1.2±0.3 (compared to 3.8±0.6 in the control group, p<0.01), demonstrating that it has obvious heat-clearing and stomach-protecting effects.

[0037] The technical solution of the present invention will be further described in detail below through embodiments. Detailed Implementation

[0038] The present invention will be further described below. It should be noted that this embodiment is based on the present technical solution and provides detailed implementation methods and specific operation processes, but the present invention is not limited to this embodiment.

[0039] Unless otherwise specified, the raw materials used in the following examples and comparative examples were all purchased directly.

[0040] Examples 1-4 all provide a formula for a herbal compound sesame oil with heat-clearing and stomach-protecting effects. The specific mass fractions are shown in Table 1.

[0041] Table 1. Herbal compound sesame oil formulations for Examples 1-4 (unit: parts)

[0042] raw material Example 1 Example 2 Example 3 Example 4 soybean oil 500 550 600 550 Sesame oil 300 350 400 350 Immortal Herb 15 16 18 15 Frangipani 10 11 12 10 buzhaye 20 21 22 20 chrysanthemum 15 16 18 15 honeysuckle 15 16 18 15 Prunella vulgaris 25 26 28 25 licorice 20 21 22 20

[0043] The preparation methods of the herbal compound sesame oil in Examples 1-4 are as follows:

[0044] (1) Herb pretreatment

[0045] After washing and air-drying the herbs such as mesona chinensis, frangipani, basil, chrysanthemum, honeysuckle, prunella vulgaris, and licorice, they are pulverized to 20-40 mesh to obtain herbal powder.

[0046] (2) Gradient simmering

[0047] Heat soybean oil to 150±5℃ and add herbal powder. Then cool it down to 120±5℃ and stir for 30-40 minutes. Finally, stop heating and let it cool naturally to below 60℃. Filter the mixture through a 200-mesh filter cloth to obtain the medicinal oil.

[0048] (3) Compound emulsification

[0049] Continue cooling the medicinal oil to 40±2℃, add sesame oil, and homogenize at 2000rpm for 5 minutes to obtain herbal compound sesame oil;

[0050] (4) Aseptic filling

[0051] The herbal compound sesame oil was nitrogen-filled and sealed in a brown glass bottle.

[0052] Comparative Example 1

[0053] The difference between this comparative example and Example 4 is that the formulation is different. This comparative example does not contain Brussels sprouts, Prunella vulgaris and Frangipani, and the total number of Brussels sprouts, Prunella vulgaris and Frangipani (55 parts) is replaced with the corresponding number of parts of sesame oil.

[0054] Comparative Example 2

[0055] The difference between this comparative example and Example 4 is that the preparation methods are different. This comparative example does not have a three-stage temperature control process. In step (2), the soybean oil is directly heated to 150±5℃ and then the herbal powder is added. Then, it is stirred at a constant temperature of 150±5℃ for 30 to 40 minutes. Finally, the heating is stopped and the oil is allowed to cool naturally to below 60℃. The oil is then filtered through a 200-mesh filter cloth.

[0056] Test case

[0057] The herbal compound sesame oils obtained in the examples and comparative examples were tested.

[0058] (1) Animal experiments

[0059] Experimental group setup:

[0060] Blank control group: SD rats were administered physiological saline (0.9% NaCl) by gavage.

[0061] Model control group: SD rats were administered commercially available sesame oil and capsaicin via gavage (simulating a spicy diet).

[0062] Experimental group: divided into three groups

[0063] a) Example 4 Product Gavage Set

[0064] b) Comparative Example 1 (three core herbs missing) gavage group

[0065] c) Comparative Example 2 (without three-stage temperature control) gavage group

[0066] Key parameters of the experimental process:

[0067] Animal model: SPF-grade SD rats (200±20g)

[0068] Dosage cycle: 14 consecutive days by gavage (once daily, 0.5 ml / 100g body weight).

[0069] Modeling method: Two hours after the last administration, administer 1.5% capsaicin solution (1 ml / animal) by gavage.

[0070] Testing indicators:

[0071] Gastric mucosal damage index (percentage of ulcer area)

[0072] Gastric mucus layer thickness (Alcian blue staining method)

[0073] Serum IL-6 levels (ELISA detection)

[0074] II. Comparison of test results between the examples and comparative examples

[0075] Gastric mucosal protective effect:

[0076] Group Ulcer area percentage Mucus layer thickness (μm) IL-6 (pg / ml) Blank control 2.1±0.3％ 45.2±3.1 18.7±2.1 Model comparison 32.6±4.8％ 22.4±2.7 86.5±7.3 Comparative Example 1 25.3±3.1％ 26.8±2.9 72.4±6.2 Comparative Example 2 19.6±2.7％ 41.3±3.6 53.7±5.1 Example 4 8.7±1.2％ 58.9±4.5 34.2±3.8

[0077] The combination of *Prunella vulgaris*, *Prunella vulgaris*, and *Frangipani* increased the thickness of the gastric mucus layer to 58.9 μm, an increase of 38.7% compared to the model group.

[0078] (2) Determination of chlorogenic acid extraction rate

[0079] The sample was determined by high performance liquid chromatography (HPLC) at a detection wavelength of 327 nm (characteristic absorption peak of chlorogenic acid).

[0080] Specific steps:

[0081] Sample pretreatment: Take 0.5g of oil sample and extract with methanol using ultrasound for 30 minutes.

[0082] Chromatographic conditions:

[0083] Chromatographic column: C18 reversed-phase column (4.6×250mm, 5μm)

[0084] Mobile phase: Acetonitrile-0.1% phosphoric acid aqueous solution (15:85)

[0085] Flow rate: 1.0 ml / min

[0086] Column temperature: 30℃

[0087] Quantitative calculation: External standard method for peak area quantification

[0088] index The process of this invention Traditional water lifting method Chlorogenic acid extraction rate 82.4％ 56.1％ Oil compatibility Stable and uniform Easy to separate into layers Activity retention rate >92％ <60％ Benzo[a]pyrene control ≤0.8μg / kg Not detected

[0089] The chlorogenic acid extraction rate reached 82.4% (compared to 56.1% by water extraction).

[0090] (3) Determination of benzo[a]pyrene content

[0091] The content of benzo[a]pyrene is ≤0.8μg / kg.

[0092] (4) Measurement of inflammatory factor levels

[0093] The product significantly reduced serum IL-6 inflammatory factor levels after consuming hot pot, as determined by ELISA (enzyme-linked immunosorbent assay). (p<0.01)

[0094] The three-stage temperature control process preserves the active ingredients, which work together to exert a synergistic anti-inflammatory effect.

[0095] (5) Stability determination

[0096] HPLC analysis showed that glycyrrhizin, chlorogenic acid, and luteolin retained >92% of their components in the oil.

[0097] No precipitates were observed after 3 months of accelerated testing (40℃ / 75%RH);

[0098] (6) Anthropometrics

[0099] A double-blind test was conducted on 50 participants, and the results after consuming this product were as follows:

[0100] The incidence of oral ulcers decreased by 76.5% (control group: commercially available sesame oil);

[0101] The heartburn score decreased to 1.2±0.3 (compared to 3.8±0.6 in the control group, p<0.01).

[0102] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A herbal compound sesame oil with heat-clearing and stomach-protecting effects, characterized in that, By weight, the herbal compound sesame oil includes 500-600 parts soybean oil, 300-400 parts sesame oil, 12-18 parts mesona chinensis, 8-12 parts frangipani, 18-22 parts buza leaves, 12-18 parts chrysanthemum, 12-18 parts honeysuckle, 22-28 parts prunella vulgaris, and 18-22 parts licorice.

2. The herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 1, characterized in that, By weight, the herbal compound sesame oil includes 550 parts soybean oil, 350 parts sesame oil, 15 parts mesona chinensis, 10 parts frangipani, 20 parts buza leaves, 15 parts chrysanthemum, 15 parts honeysuckle, 25 parts prunella vulgaris, and 20 parts licorice.

3. A method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to any one of claims 1-2, characterized in that, Includes the following steps: (1) Herb pretreatment After washing and air-drying the herbs such as mesona chinensis, frangipani, basil, chrysanthemum, honeysuckle, prunella vulgaris, and licorice, they are pulverized to obtain herbal powder. (2) Gradient refining Soybean oil is heated and then herbal powder is added. The mixture is then cooled and stirred at a constant temperature. Finally, heating is stopped and the mixture is allowed to cool naturally before being filtered to obtain the medicinal oil. The heating temperature of the soybean oil, the temperature of the constant temperature stirring, and the temperature of the natural cooling decrease in that order. (3) Compound emulsification After further cooling the medicinal oil, add sesame oil and homogenize to obtain herbal compound sesame oil. (4) Aseptic filling The herbal compound sesame oil was nitrogen-filled and sealed in a brown glass bottle.

4. The method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 3, characterized in that, In step (1), the material is pulverized to 20-40 mesh.

5. The method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 3, characterized in that, In step (2), the heating temperature of soybean oil, the constant temperature stirring temperature, and the natural cooling temperature are 140~160℃, 110~130℃, and below 60℃, respectively.

6. The method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 3, characterized in that, In step (2), filtration is performed using a 200-mesh filter cloth.

7. The method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 3, characterized in that, In step (3), continue to cool down to 35~45℃.

8. The method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 3, characterized in that, In step (3), the homogenization speed is 1000~3000 rpm and the time is 1~10 min.

9. A method for preparing a herbal compound sesame oil with heat-clearing and stomach-protecting effects according to claim 3, characterized in that, In step (3), the retention rates of glycyrrhizin, chlorogenic acid, and luteolin in the herbal compound sesame oil are >92%.

10. The application of a herbal compound sesame oil with heat-clearing and stomach-protecting effects as described in any one of claims 1-2 in the preparation of functional foods that relieve gastrointestinal stress caused by spicy diets.