Traditional Chinese medicine compound cat food capable of improving intestinal health and enhancing immunity as well as preparation method and application of traditional Chinese medicine compound cat food

Abstract

The invention belongs to the technical field of animal food processing, and particularly relates to a traditional Chinese medicine compound cat food for improving intestinal health and enhancing immunity as well as a preparation method and application thereof. The invention provides a traditional Chinese medicine compound cat food for improving intestinal health and enhancing immunity. The traditional Chinese medicine compound cat food comprises a basic ration, a compound premix and a traditional Chinese medicine formula, the basic ration comprises protein, carbohydrate, animal fat and dietary fiber; the compound premix comprises compound vitamins, compound trace mineral elements, essential amino acids, edible gels, antioxidants and mould adsorbents; the traditional Chinese medicine formula comprises artemisinic acid and / or rosmarinic acid. The traditional Chinese medicine compound cat food disclosed by the invention is comprehensive and balanced in nutrition, can provide required nutrient substances and various micronutrients for cats, improves the intestinal health, reduces the soft stool diarrhea rate, improves the organism immunity, and achieves the purposes of maintaining the body health of animals, preventing diseases, promoting disease rehabilitation and improving the life quality of pet cats.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of food processing, and particularly relates to a traditional Chinese medicine compound cat food for improving intestinal health and improving immunity, and a preparation method and application thereof. BACKGROUND

[0002] In recent years, pet cat aging, immune function decline and clinically common gastrointestinal inflammation diseases have become important problems to be faced by pet clinical veterinarians. Usually, pet treatment drugs are to lock the object target of drug action on pathogenic organisms (such as bacterial cell wall, bacterial ribosome, etc.), and ignore the use of animal body's own defense force. The modern medical mode has changed from simply treating diseases to a treatment mode combining "prevention, treatment and care", and the development trend of new treatment method is to enhance the natural immune response through non-specific pathways to improve the body's immune defense ability. The weight proportion of the digestive tract of pet cats relative to the body weight is only 2.8% to 3.5%, and the intestinal tract is relatively short, while the proportion of cat stomach in the entire digestive organ is as high as 58% to 60%, which has the problems of strong digestive capacity and weak absorption capacity, and is very easy to cause vomiting, diarrhea, constipation and other gastrointestinal diseases, and further cause the decline of immune capacity and affect the health. Due to the progressive development of pet cat immune function decline and the side effects of long-term application of chemical drugs on pets, therefore, adjusting the gastrointestinal function of pet cats and maintaining the intestinal health become the premise to ensure the healthy growth of pets, and using pet traditional Chinese medicine compound food can produce auxiliary compensatory effect on the body of early immune function decline or old pet cases, and solve the problems faced by simple western medicine treatment.

[0003] The Chinese medicine compound food for pet cats is a sub-product in the high-end cat food market, and should have the characteristics of comprehensive nutrition, high digestion and absorption rate, scientific formula, etc. As a complete pet food, it should also have the functions of auxiliary treatment and prevention of recurrence of pet diseases to ensure that pet cats obtain the necessary nutrients for basic life guarantee and growth and development. The physical properties, nutritional composition of pet food, and biochemical reactions after pet feeding will have different degrees of influence on the health status of pet cats. The existing pet cat food on the market can only provide the energy and basic nutrients required by pets in daily life, but it has little involvement in improving the immune function of pets and reducing the stress response of pets. Moreover, most of them are foreign products, and there are very few domestic self-developed compound foods for pet cats with immune function decline, gastroenteritis and other diseases. In order to meet the needs of clinical treatment of pet cats, pet compound food has become an indispensable auxiliary treatment measure in the treatment of some diseases. At present, most pet brands do not have their own production factory, and even do not have R&D personnel, but rely on the production of the factory. In order to improve production efficiency and control costs, the premix package used by the factory usually has a unified formula, which basically meets the detection requirements of domestic and international nutrition standards, but does not improve the basic nutritional conditions of pet cat food in essence, and does not conduct in-depth research on the specific proportion of each nutrient. The health of pet cats needs to rely on scientific and reasonable basic nutrition formula, balanced proportion of nutrients and stable supply, therefore, in view of the technical problems of prevention and treatment of pet cat aging, immune function decline and high mortality and high disability rate caused by clinical common gastroenteritis, the specific nutritional configuration of pet cats by means of nutritional regulation and immune regulation is helpful to alleviate the development of the disease. In view of the defects of traditional Chinese medicine prescriptions, such as many impurities of raw medicine, poor absorption, slow release of effective components, poor effect on acute cases, and inability to treat the disease, and the fact that domestic self-developed targeted compound food is rare and the research and marketing speed is slow, the market urgently needs a high-efficiency and low-toxicity therapeutic drug for pet cat aging, immune function failure and gastroenteritis. SUMMARY

[0004] Therefore, the present application aims to provide a complete Chinese medicine compound cat food for improving intestinal health and immune function, which is comprehensive and balanced in nutrition, can improve palatability and appetite, meet the needs of adult pet cats for various nutrients and multiple micronutrients, and improve intestinal health, reduce the rate of soft stool and diarrhea in cats, and improve the immune function of the body, so as to achieve the goal of increasing immunity, enhancing the health of the fur, promoting disease recovery, and improving the quality of life of pet cats.

[0005] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions:

[0006] The application provides a traditional Chinese medicine compound cat food for improving intestinal health and immunity, which comprises the following components in parts by mass: 90-110 parts of a basic daily food, 1-3 parts of a compound premix, and 0.01-0.10 parts of a traditional Chinese medicine formula.

[0007] The basic daily food comprises protein, carbohydrate, animal fat and dietary fiber;

[0008] The mass ratio of the protein, carbohydrate, animal fat and dietary fiber is 61-98:11-22:1-7:0.4-2.0;

[0009] The compound premix comprises compound vitamins, compound trace mineral elements, essential amino acids, edible gelling agents, antioxidants and mold adsorbents;

[0010] The mass ratio of the compound vitamins, compound trace mineral elements, essential amino acids, edible gelling agents, antioxidants and mold adsorbents is 28-60:12-25:10-20:2-12:4-15:8-15;

[0011] The traditional Chinese medicine formula comprises artemisinic acid and / or rosmarinic acid;

[0012] Preferably, the protein comprises the following components: chicken meat, beef, shrimp meat, chicken meat powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder and beer yeast;

[0013] The mass ratio of the chicken meat, beef, shrimp meat, chicken meat powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder and beer yeast is 32-40:2-9:1-3:20-28:3-7:2-7:0.5-1.0:0.3-0.7:1-2;

[0014] The carbohydrate comprises sweet potato powder, brown rice powder, gluten powder, carrot and pumpkin;

[0015] The mass ratio of the sweet potato powder, brown rice powder, gluten powder, carrot and pumpkin is 1.0-1.5:0.3-0.8:8-13:1-4:1-3;

[0016] The animal fat comprises chicken oil and fish oil;

[0017] The mass ratio of the chicken oil and fish oil is 1.0-6.0:0.1-0.7;

[0018] The dietary fiber comprises rice bran powder and beetroot powder;

[0019] The mass ratio of the rice bran powder and beetroot powder is 0.3-1.0:0.1-0.3.

[0020] As preferred, the complex vitamin includes the following mass concentration of components: 405-675 mcg / kg vitamin A, 3.38-5.63 mcg / kg vitamin D3, 4.5-7.5 mg / kg vitamin E, 1.35-2.25 mg / kg vitamin B1, 1.35-2.25 mg / kg vitamin B2, 13.5-22.5 mg / kg vitamin B3, 1.35-2.25 mg / kg vitamin B6, 6-10 mcg / kg vitamin B 12 , 27-45 mcg / kg D-biotin, 1.35-2.25 mg / kg D-pantothenic acid, 0.375-0.625 mg / kg folic acid;

[0021] The complex trace mineral element includes the following mass concentration of components: 15-25 mg / kg glycine copper chelate, 15-25 mg / kg methionine zinc chelate, 0.12-0.20 mg / kg calcium iodate, 15-25 mg / kg glycine iron chelate, 1.5-2.5 mg / kg methionine manganese chelate, 0.10-0.20 mg / kg selenium yeast;

[0022] The essential amino acid includes taurine and / or glutamine;

[0023] The edible gel agent is carrageenan;

[0024] The antioxidant includes potassium cinnamate and / or rosemary extract;

[0025] The mold adsorbent includes yeast cell wall extract and / or seaweed powder.

[0026] The application also provides a preparation method of the traditional Chinese medicine compound cat food, which comprises the following steps:

[0027] (1) mixing chicken, beef, shrimp meat, chicken powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder, dietary fiber and carbohydrates, crushing to obtain a crushed product; mixing the crushed product and chicken oil, steaming to obtain a mixture 1;

[0028] (2) mixing the mixture 1 and a complex premix, a traditional Chinese medicine formula to obtain a mixture 2;

[0029] (3) mixing the mixture 2 and beer yeast, fish oil to obtain the traditional Chinese medicine compound cat food.

[0030] As preferred, in step (1), the steaming time is 8-10 min; and the steaming temperature is 90-100 ℃.

[0031] Preferably, in step (2), the temperature of the mixture of the mixture 1 and the compound premix and the Chinese medicine formula is 20-30 DEG C.

[0032] The application further provides the application of the Chinese medicine compound cat food in enhancing the immunity of cats.

[0033] The application further provides the application of the Chinese medicine compound cat food in regulating the intestinal health.

[0034] Beneficial effects:

[0035] The application provides a full-price Chinese medicine compound cat food for improving intestinal health and enhancing immunity, which is composed of a basic daily ration, a compound premix and a Chinese medicine formula, is relatively balanced and comprehensive in nutrition, has a high digestion and utilization rate, and has the effects of improving intestinal health and enhancing immunity and disease resistance. The animal source / plant source raw materials and microbial protein extract in the basic daily ration can meet the various nutritional material requirements of adult pet cats for growth and development on the basis of the research on strengthening the nutritional balance of pet food; the various nutritional ingredients such as the homoeopathy carbohydrates, oils, essential amino acids and antioxidants can help in nutrient absorption, increase protein intake, promote muscle growth and repair, improve immune function, help to improve the utilization rate of the basic daily ration, regulate the metabolism of the body, enhance immunity and disease resistance, improve growth performance, promote pet hair growth, help to restore the natural color of the hair and reduce the incidence of skin diseases.

[0036] The Chinese medicine formula composed of artemisinic acid and rosmarinic acid active monomers has good water solubility, in-vitro antibacterial activity and antioxidant activity, and the combination of the two with fluoroquinolone or beta-lactam antibiotics can enhance the antibacterial effect of the antibiotics on common intestinal pathogenic bacteria, and is an ideal synergistic bacteriostatic agent. The added compound premix contains various nutritional and non-nutritional additive ingredients, which can further strengthen the nutritional value of the basic daily ration, increase the utilization rate of the effective ingredients, and be beneficial to realize special effects, enhance the resistance of adult cats to bacterial invasion, improve appetite, enhance physical fitness, maintain intestinal health and reduce the occurrence of intestinal diseases. In addition, the beer yeast and yeast cell wall extract in the basic daily ration can help to improve the intestinal microecological balance, promote the immune function of the sick animal body through the non-specific immune pathway, reduce the degree of infection, improve the cure rate, and synergistically enhance the barrier function of the intestinal mucosa of adult cats with the monomer compound, so as to maintain the health status of the animal body.

[0037] Therefore, the full-price traditional Chinese medicine compound cat food can reduce the soft stool diarrhea rate of adult cats, maintain the health of the digestive system, and improve the immune function of adult cats. Moreover, the traditional Chinese medicine compound cat food has a definite regulating effect on diarrhea, colitis, inflammatory bowel disease, indigestion, absorption disorder, and intestinal flora disorder commonly seen in adult pet cats in clinical practice. Therefore, the traditional Chinese medicine compound cat food can realize the auxiliary treatment of chronic diseases, progressive immune function decline, and common gastrointestinal diseases of pet cats, and can be effectively used in the auxiliary treatment of gastrointestinal diseases of pet cats in cooperation with a pet doctor.

[0038] The traditional Chinese medicine compound cat food provided by the present application is developed by optimizing the best mass ratio of the traditional Chinese medicine monomer formula, the probiotic extract, and the basic daily food. The traditional Chinese medicine compound cat food has the functions of treating diseases and providing nutrients. The traditional Chinese medicine compound cat food provides basic protein, fat, and carbohydrates, and additives that can improve the quality of daily food, promote the digestion and absorption of nutrients, regulate the metabolism of the body, and improve the health of animals. On the basis of strengthening the nutritional balance of pet food, the traditional Chinese medicine compound cat food manages diseases in a controlled manner, relieves the development of diseases of pet cats with aging, immune function decline, and gastrointestinal inflammation, maintains the health of the intestinal tract of cats, and improves the immune function of the body. The product provided by the present application can balance the nutrients during the treatment period by regulating the whole body from the source for specific diseases of pet cats. The product is helpful to shorten the drug treatment time, relatively reduce the drug dosage and drug side effects, and assist in the treatment of diseases, prevention of recurrence, and consideration of the nutritional and health needs of animals during the treatment period. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 The figure is a detection result of serum immune indexes, wherein CON represents a control group, and PF represents a traditional Chinese medicine compound group.

[0040] Figure 2 The figure is a detection result of serum antioxidant indexes, wherein CON represents a control group, and PF represents a traditional Chinese medicine compound group. DETAILED DESCRIPTION

[0041] The present application provides a traditional Chinese medicine compound cat food for improving intestinal health and immune function, which comprises the following components in mass parts:

[0042] The basic daily food is 90-110 parts, preferably 95-105 parts, and further preferably 100 parts;

[0043] The compound premix is 1-3 parts, preferably 1.5-2.5 parts, and further preferably 2 parts;

[0044] The traditional Chinese medicine formula is 0.01-0.10 parts, preferably 0.04-0.07 parts, and preferably 0.055 parts;

[0045] The basic daily food comprises protein, carbohydrates, animal fat, and dietary fiber.

[0046] The mass ratio of the protein, the carbohydrate, the animal fat and the dietary fiber is 61-98:11-22:1-7:0.4-2.0, preferably 70-89:15-18:3-6:0.6-1.2, and further preferably 77.5:16.7:5.0:0.8;

[0047] The compound premix comprises a compound vitamin, a compound trace mineral element, an essential amino acid, an edible gel agent, an antioxidant and a mold adsorbent;

[0048] The mass ratio of the compound vitamin, the compound trace mineral element, the essential amino acid, the edible gel agent, the antioxidant and the mold adsorbent is 28-60:12-25:10-20:2-12:4-15:8-15, preferably 35-40:15-20:10-18:4-8:5-10:10-13, and further preferably 40:18:15:6:9:12;

[0049] The traditional Chinese medicine formula comprises artemisinic acid (≥98%, HPLC) and rosmarinic acid (≥98%, HPLC); and the mass ratio of the artemisinic acid and the rosmarinic acid is 1-3:1-3, preferably 1.5-2.5:1.5-2.5, and further preferably 2:2.

[0050] Both the artemisinic acid and the rosmarinic acid have good water solubility, in-vitro antibacterial activity and antioxidant activity, and when combined with a clinically commonly used antibiotic drug for treating bacterial enteritis, have a significant synergistic antibacterial effect, can increase the sensitivity of drug-resistant bacteria to the drug, and are helpful to reduce the use amount of the antibiotic and reduce the generation of drug-resistant strains.

[0051] In the present application, the protein component comprises the following components: chicken meat, beef, shrimp meat, chicken powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder and beer yeast;

[0052] The mass ratio of the chicken meat, the beef, the shrimp meat, the chicken powder, the chicken liver, the whole egg powder, the chicken liver powder, the spirulina powder and the beer yeast is 32-40:2-9:1-3:20-28:3-7:2-7:0.5-1.0:0.3-0.7:1-2, preferably 34-38:4-7:1.5-2.5:21-24:4-6:4-5:0.7-0.9:0.4-0.6:1.3-1.7, and further preferably 36:6.8:2.0:22:3.5:4.5:0.8:0.4:1.5;

[0053] The chicken meat is fresh chicken meat, the beef is fresh beef, and the shrimp meat is fresh shrimp meat;

[0054] The chicken, beef, shrimp, chicken liver, whole egg powder, spirulina powder and beer yeast are important protein source dietary food materials (food grade), which can provide and meet the nutritional requirements of pet cats; the chicken powder (crude protein content greater than 68%, brown powder) and chicken liver powder (protein ≥ 58%, moisture ≤ 5%, yellow powder) have a delicious taste, are easy to absorb and digest, can promote animal growth, improve animal health, balance the nutritional components of daily diet, and are nutritionally comprehensive and balanced;

[0055] The carbohydrates include sweet potato powder, brown rice powder, gluten powder, carrots and pumpkins;

[0056] The mass ratio of the sweet potato powder, brown rice powder, gluten powder, carrots and pumpkins is 1.0-1.5:0.3-0.8:8-13:1-4:1-3, preferably 1.2-1.3:0.4-0.6:9-11:2.5-3.5:1.5-2.5, and further preferably 1.2:0.5:10:3:2;

[0057] The sweet potato powder, brown rice powder and gluten powder are rich in plant protein, have strong viscoelasticity and water absorption, and are beneficial to the formation of daily diet; the carrots and pumpkins are rich in nutrients, which helps to improve immunity, promote gastrointestinal peristalsis, discharge hair balls, and soften feces to prevent constipation;

[0058] The animal oil and fat includes chicken oil and fish oil;

[0059] The chicken oil is preferably Jinlao exquisite chicken oil, and the fish oil is preferably deep-sea salmon fish oil;

[0060] The mass ratio of the chicken oil and fish oil is 1-6:0.1-0.7, preferably 2-5:0.2-0.4, and further preferably 4.8:0.2;

[0061] The chicken oil can improve palatability, increase appetite, and has the effects of participating in the synthesis of phospholipids, reducing blood cholesterol levels, regulating immunity, and synthesizing precursors of important bioactive substances in the body; the fish oil is deep-sea salmon fish oil containing various fatty acids, DHA:EPA (2:3, 31%), which has the effects of strengthening bones, nourishing hair follicles, and improving skin and joint inflammation;

[0062] The dietary fiber includes rice bran powder and beetroot powder;

[0063] The mass ratio of the rice bran powder and beetroot powder is 0.3-1.0:0.1-0.3, preferably 0.5-0.8:0.1-0.2, and further preferably 0.7:0.1;

[0064] The rice bran powder and beetroot powder are rich in soluble fiber and insoluble fiber, which helps to absorb intestinal water and increase the amount of defecation.

[0065] In the present application, the complex vitamin with maltodextrin as the carrier comprises the following components in the mass concentration:

[0066] Vitamin A 405-675 mcg / kg, preferably 505-575 mcg / kg, further preferably 540 mcg / kg;

[0067] Vitamin D3 3.38-5.63 mcg / kg, preferably 4.38-4.65 mcg / kg, further preferably 4.50 mcg / kg;

[0068] Vitamin E 4.5-7.5 mg / kg, preferably 5.5-6.5 mg / kg, further preferably 6.0 mg / kg;

[0069] Vitamin B1 1.35-2.25 mg / kg, preferably 1.55-2.05 mg / kg, further preferably 1.8 mg / kg;

[0070] Vitamin B2 1.35-2.25 mg / kg, preferably 1.55-2.05 mg / kg, further preferably 1.8 mg / kg;

[0071] Vitamin B3 3.5-22.5 mg / kg, preferably 15.5-20.5 mg / kg, further preferably 18 mg / kg;

[0072] Vitamin B6 1.35-2.25 mg / kg, preferably 1.55-2.05 mg / kg, further preferably 1.8 mg / kg;

[0073] Vitamin B 12 6-10 mcg / kg, preferably 7-9 mcg / kg, further preferably 8 mcg / kg;

[0074] D-biotin 27-45 mcg / kg, preferably 32-40 mcg / kg, further preferably 36 mcg / kg;

[0075] D-pantothenic acid 1.35-2.25 mg / kg, preferably 1.55-2.05 mg / kg, further preferably 1.8 mg / kg;

[0076] Folic acid 0.375-0.625 mg / kg, preferably 0.475-0.625 mg / kg, further preferably 0.5 mg / kg;

[0077] The complex vitamin is precisely formulated, rich in 11 vitamins required by adult pet cats, has good palatability and absorption rate, and can meet the nutritional needs of adult cats;

[0078] The complex trace mineral elements take malt dextrin as a carrier and include the following components with mass concentration:

[0079] glycine copper chelate 15-25 mg / kg, preferably 18-22 mg / kg, and further preferably 20 mg / kg;

[0080] methionine zinc chelate 15-25 mg / kg, preferably 18-22 mg / kg, and further preferably 20 mg / kg;

[0081] calcium iodate 0.12-0.20 mg / kg, preferably 0.15-0.18 mg / kg, and further preferably 0.16 mg / kg;

[0082] glycine iron chelate 15-25 mg / kg, preferably 18-22 mg / kg, and further preferably 20 mg / kg;

[0083] methionine manganese chelate 1.5-2.5 mg / kg, preferably 1.8-2.2 mg / kg, and further preferably 2.0 mg / kg;

[0084] yeast selenium 0.10-0.20 mg / kg, preferably 0.13-0.17 mg / kg, and further preferably 0.15 mg / kg;

[0085] The chelate form can maximize the absorption and utilization of the body to the mineral elements, strengthen the absorption efficiency of the trace elements, be closer to the natural form of the trace elements in the body, and have higher physiological activity than the inorganic mineral elements; meanwhile, the chelate form can reduce the interaction with other nutrients such as vitamins, fatty acids, and the like, ensure the pass of the nutrition basis, improve the physical fitness of the pet cats in the disease recovery period and the old pet cats, enhance the immunity, and strengthen the constitution;

[0086] The essential amino acids include taurine and / or glutamine, and preferably taurine and glutamine; the mass ratio of the taurine and glutamine is 1-3:1, preferably 1.5-2.5:1, and further preferably 2:1;

[0087] The taurine can help the nutrient absorption, improve the immunity of the pet cats, promote the growth, and protect the vision. The glutamine can increase the protein intake, promote the muscle growth and repair, and improve the immune function;

[0088] The edible gel agent is carrageenan (food grade, content≥99%, and addition amount 0.12%-0.24%);

[0089] The carrageenan has good water retention, thickening, emulsification, and thermal reversible gelation, can make the cat food have elasticity and no increase in water separation, is beneficial to maintaining the delicate taste of the cat food, increasing the water retention capacity, and keeping the freshness of the cat food;

[0090] The antioxidant comprises potassium cinnamate and / or rosemary extract, preferably potassium cinnamate and rosemary extract;

[0091] The mass ratio of the potassium cinnamate and rosemary extract is 0.5-1.5:1, preferably 0.8-1.2:1, and further preferably 1:1;

[0092] The potassium cinnamate and rosemary extract are natural food preservatives, have good aroma preservation and oxidation delaying or hindering effects, have high antioxidant performance, good thermal stability, can prolong the storage period of food, ensure product safety, promote metabolism, and have anti-inflammatory effects.

[0093] The mold adsorbent comprises yeast cell wall extract and / or seaweed powder, preferably yeast cell wall extract and seaweed powder.

[0094] The mass ratio of the yeast cell wall extract and seaweed powder is 1-3:1, preferably 1.5-2.5:1, and further preferably 2:1.

[0095] The yeast cell wall extract is obtained by microbial degradation and belongs to a food ingredient of non-animal origin, is rich in various proteins and amino acids, and has the characteristics of freshening and flavoring, balancing odor, and strong tolerance; the seaweed powder is rich in amino acids and proteins, can improve the utilization rate of basal diet, regulate body metabolism, improve immunity and disease resistance, and promote growth; can also promote pet hair growth, help restore the natural color of hair, reduce the incidence of skin diseases, and improve skin allergy and itching; the combination of the two greatly enhances the adsorption capacity for mycotoxins, has a wide adsorption spectrum compared with inorganic adsorbents, and has fast speed and good stability, and has no interaction with nutrients such as vitamins and trace elements.

[0096] The application further provides a preparation method of the traditional Chinese medicine compound cat food.

[0097] (1) mixing chicken, beef, shrimp meat, chicken powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder, dietary fiber and carbohydrates, crushing to obtain a crushed material; mixing the crushed material and chicken oil, steaming to obtain a mixture 1;

[0098] (2) mixing the mixture 1 and a compound premix and a traditional Chinese medicine formula to obtain a mixture 2;

[0099] (3) mixing the mixture 2 and beer yeast and fish oil to obtain the traditional Chinese medicine compound cat food.

[0100] In the application, in step (1), the steaming time is 8-10 min; and the steaming temperature is 90-100 DEG C.

[0101] In the present application, in step (2), the temperature of the mixture 1 and the mixed Chinese medicine formula and the compound premix is 20-30 DEG C.

[0102] In step (2), the preparation method of the compound premix is as follows: mixing compound vitamins, compound trace mineral elements, essential amino acids, edible gel agent, antioxidant and mold adsorbent to obtain the compound premix.

[0103] The preparation method of the Chinese medicine formula is as follows: uniformly mixing artemisinic acid and rosmarinic acid to obtain the Chinese medicine monomer formula.

[0104] The step (3) after mixing further comprises sub-packaging, vacuum and refrigeration.

[0105] The present application also provides the application of the Chinese medicine compound cat food in enhancing the immunity of cats.

[0106] The present application also provides the application of the Chinese medicine compound cat food in regulating the intestinal health.

[0107] The technical solutions provided by the present application are described in detail below in combination with examples, but they should not be understood as limitations to the protection scope of the present application.

[0108] Example 1: Chinese medicine compound cat food for improving intestinal health and enhancing immunity

[0109] (1) 36 kg of fresh chicken meat, 6.8 kg of fresh beef, 2 kg of fresh shrimp meat, 22 kg of chicken powder, 3.5 kg of fresh chicken liver, 4.5 kg of whole egg powder, 0.8 kg of chicken liver powder, 0.4 kg of spirulina powder, 1.2 kg of sweet potato powder, 0.5 kg of brown rice powder, 10 kg of gluten, 3 kg of carrots, 2 kg of pumpkin, 0.7 kg of rice bran powder and 0.1 kg of beetroot powder are mixed and crushed in a meat grinder (meat particle), to obtain a crushed material; the crushed material is mixed with 4.8 kg of gold bell delicate chicken oil, and is steamed at 100 DEG C for 10 min, to obtain a mixture 1;

[0110] (2) Compound premix: mixing compound vitamins (using malt dextrin as a carrier, including the following components with the mass concentration: 540 mcg / kg of vitamin A, 4.5 mcg / kg of vitamin D3, 6 mg / kg of vitamin E, 1.8 mg / kg of vitamin B1, 1.8 mg / kg of vitamin B2, 18 mg / kg of vitamin B3, 1.8 mg / kg of vitamin B6, 8 mcg / kg of vitamin B 12, 36 mg / kg D-biotin, 1.8 mg / kg D-pantothenic acid, 0.5 mg / kg folic acid), complex trace mineral elements (including the following components with the mass concentration: 20 mg / kg glycine copper chelate, 20 mg / kg methionine zinc chelate, 0.16 mg / kg calcium iodate, 20 mg / kg glycine iron chelate, 2.0 mg / kg methionine manganese chelate, 0.15 mg / kg selenium yeast), essential amino acids (taurine: glutamine = 2:1), carrageenan, antioxidants (potassium cinnamate: rosemary extract = 1:1), and mold adsorbent (yeast cell wall extract: seaweed powder = 2:1) are mixed in a mass ratio of 40:18:15:6:9:12 to prepare a complex premix;

[0111] Traditional Chinese medicine formula: Artemisia acid and rosemary acid are mixed in a mass ratio of 1:1 to prepare a traditional Chinese medicine formula;

[0112] The mixture 1 and 1.54 kg of the compound premix and 0.06 kg of the traditional Chinese medicine formula are mixed at 25°C to obtain a mixture 2;

[0113] (3) The mixture 2 and 1.5 kg of beer yeast and 0.2 kg of deep sea salmon oil are mixed, packaged, vacuumized, and refrigerated to obtain the traditional Chinese medicine compound cat food.

[0114] Example 2: A traditional Chinese medicine compound cat food for improving intestinal health and enhancing immunity

[0115] (1) 32 kg of fresh chicken meat, 9 kg of fresh beef, 3 kg of fresh shrimp meat, 28 kg of chicken powder, 3 kg of fresh chicken liver, 2 kg of whole egg powder, 0.5 kg of chicken liver powder, 0.7 kg of spirulina powder, 1.5 kg of sweet potato powder, 0.3 kg of brown rice powder, 8 kg of gluten powder, 2 kg of carrots, 3 kg of pumpkin, 0.3 kg of rice bran powder, and 0.3 kg of beetroot powder are mixed and pulverized in a meat grinder (meat particle), to obtain a pulverized material; the pulverized material and 5 kg of Jinlao delicate chicken oil are mixed and steamed at 95°C for 8 min to obtain a mixture 1;

[0116] (2) Complex premix: complex vitamins (including the following components with the mass concentration: 405 mcg / kg vitamin A, 3.38 mcg / kg vitamin D3, 4.5 mg / kg vitamin E, 1.35 mg / kg vitamin B1, 1.35 mg / kg vitamin B2, 13.5 mg / kg vitamin B3, 1.35 mg / kg vitamin B6, 6 mcg / kg vitamin B 12, 27 mcg / kg D-biotin, 1.35 mg / kg D-pantothenic acid, 0.375 mg / kg folic acid), complex trace mineral elements (including the following components with the mass concentration: 15 mg / kg glycine copper chelate, 15 mg / kg methionine zinc chelate, 0.12 mg / kg calcium iodate, 15 mg / kg glycine iron chelate, 1.5 mg / kg methionine manganese chelate, 0.1 mg / kg yeast selenium), essential amino acids (taurine: glutamine = 2:1), carrageenan, antioxidants (potassium cinnamate: rosemary extract = 1:1), and mold adsorbent (yeast cell wall extract: seaweed powder = 2:1) are mixed in a mass ratio of 40:20:10:10:5:15 to prepare a complex premix;

[0117] Traditional Chinese medicine formula: Artemisia acid and rosemary acid are mixed in a mass ratio of 2:1 to prepare a traditional Chinese medicine formula;

[0118] The mixture 1 and 1.54 kg of the compound premix and 0.06 kg of the traditional Chinese medicine formula are mixed at 25°C to obtain a mixture 2;

[0119] (3) The mixture 2 and 1 kg of beer yeast and 0.4 kg of deep sea salmon oil are mixed, packaged, vacuumized, and refrigerated to obtain the traditional Chinese medicine compound cat food.

[0120] Example 3: A traditional Chinese medicine compound cat food for improving intestinal health and enhancing immunity

[0121] (1) 40 kg of fresh chicken meat, 2 kg of fresh beef, 1 kg of fresh shrimp meat, 20 kg of chicken powder, 7 kg of fresh chicken liver, 7 kg of whole egg powder, 1 kg of chicken liver powder, 0.3 kg of spirulina powder, 1 kg of sweet potato powder, 0.8 kg of brown rice powder, 13 kg of gluten powder, 1 kg of carrot, 1 kg of pumpkin, 1 kg of rice bran powder, and 0.2 kg of beetroot powder are mixed and crushed in a meat grinder (meat particle), to obtain a crushed material; the crushed material and 1 kg of Jinlao delicate chicken oil are mixed and steamed at 105°C for 9 min to obtain a mixture 1;

[0122] (2) Complex premix: complex vitamins (including the following components with the mass concentration: 675 mcg / kg vitamin A, 5.63 mcg / kg vitamin D3, 7.5 mg / kg vitamin E, 2.25 mg / kg vitamin B1, 2.25 mg / kg vitamin B2, 22.5 mg / kg vitamin B3, 2.25 mg / kg vitamin B6, 10 mcg / kg vitamin B 12, 45 mcg / kg D-biotin, 2.25 mg / kg D-pantothenic acid, 0.625 mg / kg folic acid), complex trace mineral elements (including the following components with a mass concentration of 25 mg / kg copper glycinate chelate, 25 mg / kg zinc methionine chelate, 0.20 mg / kg calcium iodate, 25 mg / kg iron glycinate chelate, 2.5 mg / kg manganese methionine chelate, 0.20 mg / kg selenium yeast) with malt dextrin as a carrier, essential amino acids (taurine: glutamine = 2:1), carrageenan, antioxidants (potassium cinnamate: rosemary extract = 1:1), and mold adsorbents (yeast cell wall extract: seaweed powder = 2:1) are mixed in a mass ratio of 35:15:18:12:10:10 to prepare a complex premix;

[0123] Traditional Chinese medicine formula: Artemisia acid and rosemary acid are mixed in a mass ratio of 1:2 to prepare a traditional Chinese medicine formula;

[0124] The mixture 1 and 1.54 kg of the complex premix, 0.06 kg of the traditional Chinese medicine formula are mixed at 25°C to obtain a mixture 2;

[0125] (3) The mixture 2, 2 kg of beer yeast, and 0.7 kg of deep sea salmon oil are mixed, packaged, vacuumized, and refrigerated to obtain the traditional Chinese medicine compound cat food.

[0126] Experimental Example 1: Determination of Nutritional Components

[0127] The nutritional components of the traditional Chinese medicine compound cat food described in Example 1 were determined by the national standard detection method described in Table 1, with a full-value adult commercial cat food (Qingdao Binli, Lu Shezheng (2022) 02102) as a control commercial cat food, and the determination results are shown in Table 1.

[0128] Table 1: Nutritional level detection results of the traditional Chinese medicine compound cat food

[0129]

[0130] Note: “--” indicates that it is not detected or does not reach the detection limit.

[0131] The results show that the compound cat food is comprehensive in nutrition, balanced in proportion, and scientific in formula, and can meet the needs of adult cats for various nutritional components and various trace nutrients.

[0132] Experimental Example 2: Oral acute toxicity test

[0133] The acute toxicity test was performed according to the method of the Guide to the Evaluation and Control of Veterinary Drug Residues (Veterinary Drug Evaluation and Control Center of the Ministry of Agriculture and Rural Affairs of the People's Republic of China, 2020 edition) using the up-and-down method (UDP, up-and-down or staircase method) to evaluate the toxicity of the Chinese medicine monomer artemisinic acid and rosmarinic acid; the specific steps are as follows:

[0134] Twenty BALB / c mice (20±2 g) of similar weight, half male and half female, were selected, and were isolated and acclimated for 7 days (provided by the Experimental Animal Center of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences); the drug dose was increased from 500 mg / kg to 5000 mg / kg, and was administered orally; the animal behavior changes were observed for 4 hours after administration, and the animal mortality was observed for 24 hours;

[0135] All animals were sacrificed after the end of the experiment, and gross pathological changes of important organs and tissues (heart, lung, liver, kidney, spleen, thymus, ovary / oviduct or testis, gastrointestinal tract) were observed; when obvious pathological changes were observed in these organs, histopathological examination was performed;

[0136] The results showed that no death and acute behavior changes were observed in mice orally administered with artemisinic acid and rosmarinic acid (500-5000 mg / kg) within the observation period, and no death and abnormal behavior (drowsiness, sleep, coma, tremor and diarrhea) were observed in mice; in addition, no obvious pathological changes in color and texture were observed in the important organs of mice such as heart, lung, liver, kidney, spleen, thymus, ovary / oviduct or testis, and gastrointestinal tract by gross examination, and no typical inflammatory lesions were observed; therefore, the LD 50 The estimated value is greater than 5000 mg / kg, and the toxicity is extremely low, and the clinical safe dose is high.

[0137] Experimental Example 3 Bacteriostatic effect determination

[0138] The Chinese medicine formula and its monomers described in Example 1 were selected for in vitro bacteriostatic activity determination. According to the method recommended by the American Clinical Laboratory Standardization Committee (NCCLS), the minimum bacteriostatic concentration MIC value determination of monomers, drug combinations and antibiotic drugs was performed using 96-well plate micro-broth dilution method; and the chessboard dilution method was used for fractional inhibitory concentration index (FICI) index determination.

[0139] Interpretation criteria: FICI index ≦0.5, synergistic effect; 0.5<FICI index<1, partial synergy; FICI index=1, additive effect; 1<FICI index<4, no correlation; FICI index≧4, antagonistic effect.

[0140] Strains: Escherichia coli ETEC-O78K80 (CICC 10421), Escherichia coli CICC 10302, purchased from China Industrial Microbial Culture Collection Center; Escherichia coli CMCC (B) 44102, Salmonella paratyphi B CMCC (B) 50094, purchased from China General Microbial Culture Collection Center;

[0141] Table 2 MIC values of drugs and FICI index results

[0142]

[0143]

[0144] Note: AA-arteannic acid; RA-rosemary acid; NOR-norfloxacin; P.G-penicillin sodium; OXA-oxacillin sodium; AMP-amoxicillin sodium; MDR-multi-drug resistance, multi-drug resistance, AA+RA 1:1.

[0145] The results are shown in Table 2. The antibacterial effect of active monomers alone on the four drug-resistant enterobacteriaceae is not very ideal. The combination of drugs can produce better antibacterial sensitization effect, and the synergistic effect is significant. It can also enhance the drug sensitivity of drug-resistant bacteria to antibiotics or monomers, and the effect is significantly better than that of using Chinese medicine active monomers alone or using antibiotics alone. The drug combination of arteannic acid + rosemary acid + norfloxacin has a particularly significant effect on CMCC50094 salmonella, and the antibacterial effect is doubled, which can reduce the dosage of monomers and norfloxacin single drug, effectively reverse bacterial drug resistance, and has a synergistic effect.

[0146] Experimental Example 4 Palatability experiment

[0147] Test animal design: The experiment was carried out in the pet cat house of Dawashan Experimental Animal Farm of Lanzhou Animal Husbandry and Veterinary Institute of Chinese Academy of Agricultural Sciences; 12 growing period English short blue cats (half male and half female, body weight 2.5-4.0 kg) were selected and divided into 2 groups according to gender and body weight; each group had basically the same age, and the male and female were kept in balance (3 males and 3 females), 6 replicates per group, 1 cat per replicate, and single cage feeding; the temperature in the cat house was room temperature; the drinking water, food utensils were cleaned daily, and the cat litter was replaced; the feeding environment and utensils were disinfected every day; all test cats were immunized and dewormed before the test; two kinds of cat food used in the test were: Chinese medicine compound cat food prepared in Example 1 of the application (Chinese medicine compound group), and the original complete adult cat food in the cat house (Qingdao Binli, Luhe certificate (2022) 02102) (control group).

[0148] Cat food palatability test: The "double pot method" was used to determine the test, and the experimental design was 6 groups of comparative test (traditional Chinese medicine compound cat food and control complete adult cat food), 6 replicates per group, 1 cat per replicate (single cage feeding); 80g of each cat food was weighed each time and placed in two identical cat bowls, and the two cat bowls were given to the cat at the same time, and the cat was allowed to drink water freely.

[0149] The preferred daily diet of each cat for the two cat foods was recorded during feeding, and the recording was completed quickly, and as much as possible to ensure that the test cat was not disturbed by human factors during feeding; after 1h of feeding, the remaining food in the two cat bowls was weighed, and the first mouth preference, feeding rate and feeding amount of the two cat foods were calculated; the positions of the two cat bowls were exchanged each time, the pre-feeding period was 2d, and the test period was 3d, and the results of the cat food palatability test are shown in Table 3;

[0150] The calculation formulas of the first mouth preference and the feeding rate are as follows:

[0151] The first mouth preference (%) = the number of times that the test cat selects one cat food / the total number of tests x 100, and the feeding rate (%) = the feeding amount of one cat food / the total feeding amount of two cat foods x 100;

[0152] Data processing and statistical analysis:

[0153] All test data were input and preliminarily arranged by Excel (2019), and single factor ANOVA analysis and Duncan method multiple comparison significance analysis, univariate or multivariate analysis of general linear model, and Pearson correlation test were performed by SPSS 26.0 software. The analyzed data were processed and plotted by GraphPad Prism 9 software. The statistical results were represented by Mean ± S.E., and P<0.05 was used as the significant difference judgment standard, P<0.01 represented extremely significant difference, and 0.05≤P<0.10 represented a significant difference trend.

[0154] Table 3 Cat food palatability test results (Mean ± S.E., n = 6)

[0155] Index Control group Traditional Chinese medicine compound group P value C.I.(95%) First choice of diet(%) 42.80±6.57 57.80±7.12 0.009 43.00-57.60 Intake(g / time) 21.33±9.34 39.63±6.35 0.002 22.39-38.58 Intake rate(%) 32.26±3.69 67.74±3.69 0.000 36.40-63.60

[0156] As can be seen from Table 3, the first mouth preference, feeding amount and feeding rate of the traditional Chinese medicine compound group were extremely significantly higher than those of the control group (P<0.01), therefore, the compound cat food can significantly improve the palatability of cat food and increase the appetite.

[0157] Experimental Example 5 Feeding experiment and clinical test

[0158] The test animal design refers to Experimental Example 4. Seven days before the start of the test, all cats in the traditional Chinese medicine compound group were gradually transitioned to feeding the traditional Chinese medicine compound cat food using a stepwise feed change method; the control group continued to be fed the original cat food; after the pre-feeding ended, a 35-day feeding test was conducted, the traditional Chinese medicine compound group was fed the traditional Chinese medicine compound cat food described in Example 1, and the control group was fed the original complete adult cat food in the cat house (Qingdao Binli, Lu Shi Zhen (2022) 02102).

[0159] During the formal test period, the cat food was weighed and fed at a fixed time every day, and the remaining amount was recorded the next day. The feed intake was the feeding amount minus the remaining amount. The water was weighed and recorded at a fixed time every day, and the remaining amount was recorded the next day. The drinking water was the added water minus the remaining amount. Before the start of the formal test (0d), on the day of the end of the test (35d), and every 7 days (7d, 14d, 21d, 28d), each test cat was weighed and recorded individually before feeding, which was used for the analysis of the growth performance of the test cats. During the whole test, the test cats were observed for constipation, diarrhea, fecal hair balls, and general clinical symptoms. The quality of the cat's fur was detected every week, and the cat's hair length was measured before and after feeding (the evaluation standard is shown in Table 4).

[0160] The diarrhea rate was calculated as follows: diarrhea rate = number of times of diarrhea / (number of test cats x number of test days).

[0161] Table 4 Evaluation standard for pet cat hair

[0162]

[0163] During the test period, the cats were scored for feces (FS, Fecal score) every day, using the Waltham 5-point feces scoring method: 1.0≤FS<2.0 was determined as constipation and hard feces, 2.0≤FS≤3.0 was determined as well-formed normal feces, 3.0<FS<4.0 was determined as unformed soft stool, and 4.0≤FS≤5.0 was determined as diarrhea and watery stool.

[0164] From the 24th day to the 35th day of the test, the total weight of urine clumps of each test cat was collected and weighed at a fixed time every day (before feeding and water changing). The daily urine output of the test cats was calculated accordingly. The feces of each test cat were collected at a fixed time every day (before feeding and water changing), weighed, and stored at -20℃ for apparent digestibility analysis. The detection methods for each index are shown in Table 2.

[0165] Nutrient apparent digestibility (%) = [1-(A / B x C / D)] x 100

[0166] In the formula: A, the content of a nutrient in feces (%); B, the content of the corresponding nutrient in the diet (%); C, the content of acid-insoluble ash in the diet (%); D, the content of acid-insoluble ash in feces (%).

[0167] Data processing and statistical analysis: same as described in Example 4.

[0168] Results and analysis:

[0169] (1) The growth performance results of the test cats are shown in Table 5. There was no significant difference in the initial body weight and final body weight between the Chinese medicine compound group and the control group (P>0.05); compared with the control group, the compound cat food group had obvious weekly weight gain effect in the later feeding period, and the weight gain in the 4th week and the 5th week was particularly significant; secondly, the Chinese medicine compound group was also significantly better than the control group in terms of daily weight gain, daily feed intake and feed conversion ratio (P<0.05, P<0.01).

[0170] Table 5 Analysis results of Chinese medicine compound cat food on production performance (Mean ± S.E., n=6)

[0171]

[0172] (2) The feces score and clinical observation results are shown in Table 6. Compared with the control group, the addition of Chinese medicine compound in the diet can significantly reduce the feces score of adult cats (P<0.01), and maintain a relatively healthy value range (2.26±0.15) during feeding, indicating that the addition of the Chinese medicine compound in the diet helps to maintain the feces score of adult cats within the normal index range; the adult cats in the control group showed obvious symptoms of unformed loose stool and diarrhea in the middle feeding period, with a feces score of 3.50-4.50, and the symptoms improved in the later feeding period, but the water content in the feces was still high, and the feces score was still at a high level, among which the clinical symptoms of diarrhea in adult male cats were more obvious than in female cats. Secondly, there were significant differences in the amount of excrement, water intake and urine output between the two groups (P<0.01), and the adult cats in the control group showed the characteristics of increased water intake and decreased urine output, while the increased water content in the feces led to a significantly higher amount of excrement than the Chinese medicine compound group (P<0.01).

[0173] On the 35th day of the feeding experiment, the coat quality score of the adult cats in the Chinese medicine compound group was significantly better than that of the control group (P<0.01), indicating that the Chinese medicine compound cat food can promote the maintenance of skin barrier function and hair luster; but it has no significant effect on the hair length of adult cats (P>0.05), and the Pearson correlation test results also show that there is no significant correlation between different cat food feeding and hair length. Therefore, the Chinese medicine compound cat food can not only strengthen the nutritional balance of adult cats, but also moisturize the skin, enhance the skin health, and significantly improve the symptoms of dry and dull hair, and comprehensively promote the health of the coat.

[0174] Table 6 Effect of Chinese medicine compound cat food on the clinical condition and coat quality of the test cats (Mean ± S.E., n=6)

[0175] Index Control group Traditional Chinese medicine compound group P value C.I.(95%) Fecal score 3.17±0.28 2.26±0.15 0.000 2.60-2.83 Excrement output(g / d) 118.90±23.51 55.73±13.79 0.000 71.53-103.09 Water intake(mL / d) 166.74±20.99 141.30±17.39 0.000 148.61-159.43 Urine output(mL / d) 73.88±13.48 124.46±8.42 0.000 82.79-115.56 Diarrhea rate(%) 41.89 0.00 -- -- Skin quality score before feeding(cm, 0d) 194.58±15.56 212.08±12.93 0.396 182.52-224.15 Skin quality score after feeding(cm, 35d) 204.17±11.23 243.75±7.52 0.008 207.84-240.07 Hair length before feeding(cm, 0d) 3.66±0.05 3.68±0.07 0.807 3.58-3.76 Hair length after feeding(cm, 35d) 3.74±0.09 3.76±0.13 0.874 3.59-3.91

[0176] (3) The results of the apparent digestibility of nutrients of different cat foods are shown in Table 7. Compared with the control group, the apparent digestibility of crude protein, crude fat and total phosphorus of the cat food in the Chinese medicine compound group was significantly higher than that of the control group (P<0.05), and there was a significant difference trend in dry matter (P=0.078) and crude ash (P=0.053); there was no significant difference in the apparent digestibility of crude fiber and total calcium between the two groups (P>0.05); the results of nutrient digestibility showed that the cat food in the Chinese medicine compound group was easier to digest and absorb than the control group, and could significantly improve the apparent digestibility of most nutrients.

[0177] Table 7 Effect of different cat food treatments on the apparent digestibility of nutrients of cats (Mean ± S.E., n=6)

[0178] Index Control group Traditional Chinese medicine compound group P value C.I.(95%) Dry matter(%) 82.95±1.44 86.45±0.38 0.078 82.06-87.34 Crude protein(%) 81.71±3.49 91.53±0.37 0.049 79.68-93.56 Crude fat(%) 86.38±1.25 91.72±1.20 0.037 85.39-92.71 Crude fiber(%) 66.16±6.28 80.09±3.43 0.124 61.65-84.61 Crude ash(%) 26.23±6.81 45.03±1.20 0.053 22.22-49.04 Total calcium(%) 50.87±8.80 65.41±2.60 0.188 44.68-71.60 Total phosphorus(%) 70.25±2.70 78.15±0.78 0.048 68.63-79.78

[0179] Sample collection and detection indexes:

[0180] On the 30th day before feeding, blood was collected from the forelimb vein, serum was separated, and five classification blood routine (animal special automatic blood cell analyzer, BC-40R Vet, Shenzhen Mindray Animal Medical Technology Co., Ltd.), serum biochemistry / serum minerals / electrolytes (animal special automatic biochemical immune analyzer Mindray vetXpertCubeC5, Shenzhen Mindray Animal Medical Technology Co., Ltd.), serum immune indexes (IgG, IL-4, IL-6, IFN-γ, IgA, TNF-α) and antioxidant indexes (D-lactic acid D-Lac, diamine oxidase DAO, malondialdehyde MDA, total antioxidant capacity T-AOC) (enzyme-linked immunoassay ELISA or biochemical method) were detected.

[0181] The results of five classification blood routine, serum biochemistry, serum minerals and electrolytes are shown in Tables 8-10, the detection results of serum immune indexes are shown in Figure 1 , and the antioxidant indexes are shown in Figure 2 .

[0182] (4) As shown in Table 8, no significant difference was observed in the 20 blood routine indexes between the two groups of adult cats (P>0.05), but significant difference was observed in the total number of white blood cells, neutrophil and monocyte (P<0.05), and the control group had a tendency of obvious increase compared with the Chinese medicine compound group, which was presumed to be related to the continuous unformed loose stool of the male cats in the control group in the later stage of the feeding experiment; in addition, the hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin and reticulocyte indexes of the two female cats in the control group were significantly lower than the normal value range, which was considered to be caused by anemia before regenerative response; the above index results showed that the nutrient components contained in the Chinese medicine compound cat food could improve and strengthen the body constitution of adult cats, and prevent the occurrence of digestive tract inflammation or diarrhea.

[0183] Table 8 Effects of different cat foods on blood routine indexes of the test cats (Mean ± S.E., n=6)

[0184]

[0185] (5) As shown in Table 9, the total protein TP, albumin ALB, globulin GLOB (slightly higher than the normal value range in the control group), albumin / globulin ratio A / G, alanine aminotransferase ALT, aspartate aminotransferase AST, AST / ALT, alkaline phosphatase ALP, glutamyl transferase GGT, total bilirubin TBIL, amylase AMY, urea nitrogen BUN, BUN / creatinine ratio BUN / CREA, lactate dehydrogenase LDH and total cholesterol TC levels in the serum of the adult cats were not significantly affected by different cat foods, and the above indexes were within the normal reference value range; in addition, the serum urea nitrogen, creatinine and total cholesterol levels in the Chinese medicine compound group were significantly higher than those in the control group (0.05≤P<0.10), the serum creatinine level in the control group was significantly lower than the normal value range (45.13-179.65 μmol / L), and the creatine kinase level was significantly higher than the normal value range (66.10-530.90 U / L), which was presumed to be related to the long time of cage breeding, less activity of the test cats, and insufficient intake of daily food or protein loss enteropathy caused by chronic and persistent enteritis. In this study, the serum glucose GLU levels of the test cats in the two groups were lower than the normal value level (3.39-8.39 mmol / L), indicating that the two cat foods used in the test could reduce the blood glucose concentration and improve the glucose metabolism of adult cats; in summary, the addition of Chinese medicine compound in the daily food would not cause adverse effects on the blood of adult cats.

[0186] Table 9 Effects of fresh meat replacing meat powder on serum biochemical indexes of the test cats (Mean ± S.E., n=6)

[0187]

[0188]

[0189] (6) As shown in Table 10, there were no significant differences in the two mineral indicators among the different cat food treatments (P>0.05), and all of the above indicators were within the normal reference range; however, there were significant differences between the two groups in the calcium-phosphorus product (P<0.05); the serum calcium (Ca), phosphorus (PHOS), and calcium-phosphorus product (Ca×P) of the traditional Chinese medicine compound group were all higher than those of the control group, which suggests that the calcium and phosphorus content of the cat food in this group or the mineral intake of the experimental cats was higher than that of the control group; in addition, there were no significant differences in the five electrolyte indicators among the different cat food treatments (P>0.05), except for the serum potassium (K) in the control group. + Except for the levels exceeding the normal reference range, all other levels are within the normal reference range.

[0190] Table 10 Effects of different cat foods on serum mineral and electrolyte levels in experimental cats (Mean±SE, n=6)

[0191] Index Control group Traditional Chinese medicine compound group P value C.I.(95%) Calcium Ca(mmol / L) 2.18±0.04 2.30±0.05 0.090 2.17-2.32 Phosphorus PHOS(mmol / L) 1.77±0.08 2.02±0.10 0.078 1.74-2.05 Calcium-phosphorus product Ca×P(mmol / L^2) 3.88±0.21 4.64±0.19 0.023 3.87-4.65 total carbon dioxide TCO2 (mmol / L) 14.54±1.16 16.90±0.47 0.089 14.19-17.25 Sodium ion Na + (mmol / L) 150.40±3.04 150.35±2.36 0.990 146.34-154.41 Potassium ion K + (mmol / L) 5.78±0.26 5.17±0.19 0.084 5.08-5.87 Sodium-potassium ratio Na / K 26.15±1.32 29.37±1.56 0.146 25.37-30.15 Chloride ion Cl - (mmol / L) 113.38±3.10 112.97±1.28 0.904 109.65-116.70

[0192] (7) Serum immune marker results (such as...) Figure 1 The herbal compound cat food exerts antibody activity by increasing the levels of immunoglobulins IgG and IgA. It plays a crucial role in maintaining intestinal health in adult cats and regulating the intestinal mucosal immune response, serving as the first line of defense against pathogen invasion. Compared to the control group, the herbal compound group showed significantly higher levels of serum IL-4 and lower levels of IL-6, suggesting that the herbal compound cat food enhances cell-mediated immune defense and exerts an anti-inflammatory effect to some extent. The herbal compound group also showed significantly lower serum IFN-γ levels in adult cats, while there was no significant difference in TNF-α levels between the two groups, indicating that the herbal compound cat food can enhance immunity and reduce inflammation to some extent. Effects: It is evident that long-term feeding with the herbal compound cat food prepared in Example 1 significantly increased the serum levels of IgG and IL-4 in adult cats in the experimental group, and decreased the serum levels of IL-6 and IFN-γ, with significant differences between groups (P < 0.05, P < 0.01). However, there were no significant differences in the levels of IgA and TNF-α between groups, indicating that the herbal compound cat food used in this study has a certain regulatory effect on the immune function of adult cats. Therefore, this herbal compound cat food, through the formulation of herbal monomers and in combination with probiotic by-products in the basic diet, can improve the intestinal microecological balance, reduce the rate of soft stool diarrhea, and increase the serum immunoglobulin content, thereby achieving the purpose of enhancing immunity, disease resistance, and strengthening the body.

[0193] (8) Serum antioxidant results (e.g.) Figure 2), at the end of the test on the 30th day, compared with the control group, the T-AOC (total antioxidant capacity) level of the traditional Chinese medicine compound group was significantly increased (P<0.05), and the MDA (malondialdehyde) content was increased, but the difference between groups was not obvious, indicating that the traditional Chinese medicine compound cat food had a certain effect on the antioxidant enzyme level of the serum of adult cats; secondly, the traditional Chinese medicine compound cat food can significantly reduce the serum D-lactic acid (D-Lac) and diamine oxidase (DAO) levels, and the traditional Chinese medicine compound cat food can help to competitively inhibit the growth of harmful microorganisms, reduce inflammation and histamine production, indicating that the integrity of the intestinal barrier has been improved.

[0194] Therapeutic effect comparison test of traditional Chinese medicine compound cat food in Example 6

[0195] The test was carried out in the pet cat house of the Dawushan Experimental Animal Farm of the Lanzhou Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences. The inclusion criteria for the test animals were: adult cats with continuous unformed soft stool for about 3 days, fecal score FS≥4.00; the specific therapeutic effect comparison test was to randomly divide 12 adult cats meeting the inclusion criteria into two groups, each cat was fed in a single cage, and cold boiled water was freely available, and the remaining conditions were kept consistent; the treatment group was fed with the traditional Chinese medicine compound cat food prepared in Example 1, and the non-treatment group was fed with the original complete adult cat food (Qingdao Binli, Lu She Zhen (2022) 02102) in the cat house. During the test feeding period, the adult cats were observed daily for diarrhea, constipation, gastrointestinal inflammation and other clinical symptoms, fecal scoring was performed daily, daily feed intake was calculated, and the number of fecal hair balls was observed, and the results are shown in Table 11.

[0196] Table 11 Therapeutic effect of traditional Chinese medicine compound cat food on the clinical symptoms of diarrhea and soft stool in adult cats

[0197]

[0198] As shown in Table 11, after treatment, the fecal score of the adult cats in the traditional Chinese medicine compound cat food treatment group showed a decreasing trend on the 2nd day, the spirit and food appetite were enhanced, and the feed intake was significantly increased; on the 3rd day, the fecal score returned to the normal value range, and the unformed soft stool basically disappeared, showing soft stool and normal formation; on the 4th day, long columnar feces with good formation were observed, and individual fecal particles contained varying amounts of hair balls. During the test treatment period, there was no recurrence of symptoms such as diarrhea and gastrointestinal inflammation, and the effective rate and cure rate were both 100%; the 4 adult cats in the control group showed no significant improvement in daily feed intake, mental state, food and water intake, and the fecal score remained around 4.00, and showed persistent diarrhea and unformed soft stool, and no fecal hair balls were observed during the period. Therefore, the traditional Chinese medicine compound cat food prepared by the present application has good therapeutic effect on the symptoms of diarrhea and gastrointestinal inflammation in adult cats, can reduce the degree of infection, enhance appetite, improve the cure rate, and accelerate the recovery of physical condition.

[0199] From the above examples, the present application provides a traditional Chinese medicine compound cat food for improving intestinal health and improving immunity, and a preparation method and application thereof. The traditional Chinese medicine compound cat food is comprehensive and balanced in nutrition, can improve palatability, increase appetite, meet the needs of adult pet cats for various nutrients and various trace nutrients, and can improve intestinal health, reduce the soft stool and diarrhea rate of cats, and improve the immunity of the body, so as to achieve the goals of increasing immunity, resisting diseases, enhancing the health of fur, promoting disease recovery, and improving the life quality of pet cats.

[0200] The above only describes the preferred embodiments of the present application, and it should be noted that for ordinary skilled persons in the art, several improvements and refinements can be made without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A traditional Chinese medicine compound cat food that improves gut health and enhances immunity, characterized in that, It includes the following components by weight: 90-110 parts of basal diet, 1-3 parts of compound premix, and 0.01-0.10 parts of traditional Chinese medicine formula; The basal diet includes protein, carbohydrates, animal fats, and dietary fiber; The mass ratio of protein, carbohydrates, animal fats, and dietary fiber is 61–98:11–22:1–7:0.4–2.0; The compound premix includes compound vitamins, compound trace mineral elements, essential amino acids, edible gelling agents, antioxidants, and mold adsorbents. The mass ratio of the compound vitamins, compound trace mineral elements, essential amino acids, edible gelling agents, antioxidants, and mold adsorbents is 28-60:12-25:10-20:2-12:4-15:8-15; The traditional Chinese medicine formula includes artemisinic acid and / or rosmarinic acid.

2. The herbal compound cat food according to claim 1, characterized in that, The protein comprises the following components: chicken, beef, shrimp, chicken powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder, and brewer's yeast; The mass ratio of chicken, beef, shrimp, chicken powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder, and brewer's yeast is 32-40:2-9:1-3:20-28:3-7:2-7:0.5-1.0:0.3-0.7:1-2; The carbohydrates include sweet potato flour, brown rice flour, wheat gluten, carrots, and pumpkin; The mass ratio of sweet potato flour, brown rice flour, wheat gluten, carrot, and pumpkin is 1.0–1.5: 0.3–0.8: 8–13: 1–4: 1–3; The animal fats include chicken fat and fish oil; The mass ratio of chicken oil to fish oil is 1.0–6.0:0.1–0.5; The dietary fiber includes rice bran powder and beetroot powder; The mass ratio of rice bran powder to beetroot powder is 0.3-1.0:0.1-0.

3.

3. The herbal compound cat food according to claim 2, characterized in that, The compound vitamin, using maltodextrin as a carrier, comprises the following components at the following mass concentrations: 405–675 mcg / kg Vitamin A, 3.38–5.63 mcg / kg Vitamin D3, 4.5–7.5 mg / kg Vitamin E, 1.35–2.25 mg / kg Vitamin B1, 1.35–2.25 mg / kg Vitamin B2, 13.5–22.5 mg / kg Vitamin B3, 1.35–2.25 mg / kg Vitamin B6, and 6–10 mcg / kg Vitamin B1. 12 27–45 mcg / kg D-Biotin, 1.35–2.25 mg / kg D-Pantothenic Acid, 0.375–0.625 mg / kg Folic Acid; The complex trace mineral elements, using maltodextrin as a carrier, comprise the following components at the following mass concentrations: 15–25 mg / kg glycine copper chelate, 15–25 mg / kg methionine zinc chelate, 0.12–0.20 mg / kg calcium iodate, 15–25 mg / kg glycine iron chelate, 1.5–2.5 mg / kg methionine manganese chelate, and 0.10–0.20 mg / kg yeast selenium; The essential amino acids include taurine and / or glutamine; The edible gelling agent is carrageenan; The antioxidants include potassium cinnamate and / or rosemary extract; The mold adsorbent includes yeast cell wall extract and / or seaweed powder.

4. The method for preparing the traditional Chinese medicine compound cat food according to any one of claims 1 to 3, characterized in that, Includes the following steps: (1) Mix chicken, beef, shrimp, chicken powder, chicken liver, whole egg powder, chicken liver powder, spirulina powder, dietary fiber and carbohydrates, grind them to obtain a powder; mix the powder with chicken fat and steam to obtain mixture 1; (2) Mix mixture 1 with the compound premix and the traditional Chinese medicine formula to obtain mixture 2; (3) Mix mixture 2 with brewer's yeast and fish oil to obtain traditional Chinese medicine compound cat food.

5. The preparation method according to claim 4, characterized in that, In step (1), the steaming time is 8 to 10 minutes; the steaming temperature is 90 to 100°C.

6. The preparation method according to claim 5, characterized in that, In step (2), the temperature at which the mixture 1, the compound premix, and the traditional Chinese medicine formula are mixed is 20-30°C.

7. The use of the traditional Chinese medicine compound cat food according to any one of claims 1 to 3 in enhancing cat immunity.

8. The application of the traditional Chinese medicine compound cat food according to any one of claims 1 to 3 in regulating intestinal health.

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