An integrated reagent tube tray and its usage method

Abstract

This invention discloses an integrated reagent tube tray and its usage method. It includes: an integrated reagent tube tray adapted to colorimetric instruments, fluorescence instruments, luminescence instruments, and imaging instruments; comprising: a support and a plurality of reagent tubes; the support having a plurality of perforated portions spaced apart; the reagent tubes being detachably disposed within the perforated portions; each perforated portion corresponding to a plurality of reagent tubes; and a connecting beam extending from the bottom left and right sides of each reagent tube toward the support; each reagent tube having at least one reaction cavity for reaction detection and at least one reactant cavity for containing reactants; and each reagent tube having an detachably disposed opening with an independent sealing membrane for shielding the reaction cavity and the reactant cavity; this invention simplifies the reagent manufacturing process and also simplifies the automated instrument operation process of sample addition, reaction, and detection.

Description

Technical Field

[0001] This invention relates to the field of biological liquid detection and analysis technology, and in particular to an integrated reagent tube tray and its usage method. Background Technology

[0002] Multi-optical analysis refers to the use of various techniques such as colorimetry, fluorescence, luminescence, and imaging to detect and analyze target substances in liquid samples based on changes in light signals. It is widely used for qualitative and quantitative analysis of multiple target substances in biological liquid samples.

[0003] Currently, biological liquid sample analysis technology is developing towards convenience, miniaturization, integration, dry processing, full automation, high sensitivity, and appropriate throughput, while simultaneously acquiring multiple targets and information in the sample.

[0004] In the development of biological liquid sample analysis technology, full automation helps stabilize conditions, ensuring stable and reproducible results, and also reduces the workload of manual operation. Traditional single-analytical methods, due to their early technological development, had each module designed for optimal operation, resulting in a self-contained solution with numerous structural modules, reagent components, and operational steps. This resulted in high costs, cumbersome operation, and a relatively high failure rate. Furthermore, the instruments occupied a large space, causing inconvenience to users.

[0005] In the fully automated process of biological liquid sample analysis, the handling of reaction vessels and the dispensing of liquid reagents are always required. Using conventional tube organizers to arrange and organize reaction vessels and dispensing devices occupies instrument space, while manually arranging them on racks before processing consumes laboratory time. The shapes of reaction vessels vary greatly across different testing platforms, and the dispensing methods differ accordingly. Consequently, clinical testing centers have a multitude of devices for various testing methods, with incompatible reaction vessels, increasing testing costs. Liquid reagents require cold chain storage and transportation, and the time spent on dispensing and cleaning sampling needles during testing further complicates the instrument's structure.

[0006] Therefore, developing a dry integrated reactor that can meet the high-throughput detection requirements of various optical analyses is of great practical significance, so as to promote the development of detection and testing devices towards full automation, high throughput, dryness, miniaturization and integration. Summary of the Invention

[0007] To address the problems existing in the prior art, the present invention provides an integrated reagent tube tray and its usage method, which can effectively solve the problems existing in the prior art.

[0008] The technical solution of this invention is:

[0009] According to one aspect of the present invention, an integrated reagent tube tray is adapted to colorimetric instruments, fluorescence instruments, luminescence instruments, and imaging instruments, comprising: a support and a plurality of reagent tubes, wherein the support is provided with a plurality of perforated portions at intervals, the reagent tubes are detachably disposed in the perforated portions, the plurality of perforated portions corresponding one-to-one with the plurality of reagent tubes, and a connecting beam extending toward the support from the bottom left and right sides of each reagent tube; each reagent tube is provided with at least one reaction cavity for reaction detection and at least one reactant cavity for containing reactants; each reagent tube opening is detachably provided with an independent sealing membrane for shielding the reaction cavity and the reactant cavity; the support, the plurality of reagent tubes, and the plurality of connecting beams are integrally formed; the reagent tubes are separated from the support by an automatic shearing extraction method to realize single-sample single-test or single-sample multi-index test detection, obtain target information, and perform qualitative and quantitative analysis.

[0010] Furthermore, the bracket has several positioning posts extending upward from its corners, and each positioning post has a positioning groove at its lower end. When the integrated reagent tube trays are stacked, the positioning posts of the bracket are inserted into the positioning grooves.

[0011] Furthermore, the outer periphery of the support is provided with a number of grooves at intervals. These grooves are used to provide clamping points for the clamping device of the reagent tube tray integrated into the fully automatic operation of the reagent manufacturing equipment and testing instrument, so as to achieve precise docking and positioning.

[0012] Furthermore, each reagent tube has a connecting beam symmetrically spaced at the bottom of its left and right sides. The connecting beam is in the shape of a horizontal strip, and the reagent tube is connected to the support through the connecting beam. The cross-section of the connecting beam is triangular, rhomboid, trapezoidal, triangular groove, trapezoidal groove, or cylindrical. The length of the connecting beam is 0.3mm to 3mm, and the width and height are both no more than 2mm.

[0013] Further, the reagent tube includes a first reagent tube body, a first reaction lumen, and a first reactant lumen. The first reaction lumen contains a dry reactant of a solidified antigen, a solidified antibody, or other target substance. The first reactant lumen contains a label of the antigen, a label of the antibody, or a dry reactant of a matching reactant corresponding to the aforementioned other target substance. The first reaction lumen and the first reactant lumen are disposed at an interval between the front and rear of the first reagent tube body, and the upper end face of the first reaction lumen is flush with the upper end face of the first reactant lumen.

[0014] The cross-section of the first reagent tube is elliptical, the cross-section of the first reaction tube is circular, the cross-section of the first reactant tube is crescent-shaped, and the concave surface of the first reactant tube faces the first reaction tube.

[0015] The major semi-axis a of the ellipse of the first reagent tube is 4.5 mm to 7.5 mm, the minor semi-axis b is 3.6 mm to 4.4 mm, and the eccentricity e is 0.6 to 0.88; the diameter of the first reaction tube lumen is 3 to 9 mm.

[0016] The length of the hollowed-out portion is 12mm~17mm, and the width is 9mm~11mm.

[0017] Further, the reagent tube includes a second reagent tube body, a second reaction lumen, a second reactant lumen, and a sixth reactant lumen. The second reaction lumen contains a dry reactant of a solidified antigen, a solidified antibody, or other target substance. The second reactant lumen contains a label of the antigen, a label of the antibody, or a dry reactant of a matching reactant corresponding to the aforementioned other target substance. The sixth reactant lumen is configured as a sample processing tube for centrifuging whole blood samples to convert them into plasma or serum samples for use as detection samples. The sixth reactant lumen, the second reactant lumen, and the second reaction lumen are sequentially spaced from front to back within the second reagent tube body, and the upper surfaces of the sixth reactant lumen, the second reactant lumen, and the second reaction lumen are flush.

[0018] The cross-section of the second reagent tube is elliptical, the cross-section of the second reaction tube is circular, and the cross-sections of the second reactant tube and the sixth reactant tube are both crescent-shaped. The crescent-shaped concave surfaces of the sixth reactant tube and the second reactant tube overlap and interlock on one side of the second reaction tube, and the crescent-shaped concave surfaces all face the second reagent tube.

[0019] The second reagent tube has a major semi-axis a of 4.5 mm to 7.5 mm, a minor semi-axis b of 3.6 mm to 4.4 mm, and an eccentricity e of 0.6 to 0.88; the diameter of the second reaction tube lumen is 3 to 9 mm.

[0020] The length of the hollowed-out portion is 12mm~17mm, and the width is 9mm~11mm.

[0021] Further, the reagent tube includes a third reagent tube body, a third reaction lumen, and at least two third reactant lumens. The third reaction lumen contains a dry reactant of a solidified antigen, a solidified antibody, or other target substance. Each third reactant lumen contains a label of the antigen, a label of the antibody, or a dry reactant of a matching reactant corresponding to the aforementioned other target substance. The third reaction lumen is located at the axis of the third reagent tube body, and the two third reactant lumens are symmetrically mirrored on the front and rear sides of the third reaction lumen, and the upper surfaces of the third reaction lumen are flush with the upper surfaces of the two third reactant lumens.

[0022] The cross-section of the third reagent tube is elliptical, the cross-section of the third reaction tube is circular, the cross-section of the third reactant tube is crescent-shaped, and the concave surface of the third reactant tube faces the third reaction tube.

[0023] The major semi-axis a of the ellipse of the third reagent tube is 4.5mm~7.5mm, the minor semi-axis b is 3.6mm~4.4mm, and the eccentricity e is 0.6~0.88.

[0024] The length of the hollowed-out portion is 12mm~17mm, and the width is 9mm~11mm.

[0025] Further, the reagent tube includes a fourth reagent tube body, a fourth reaction lumen, and at least two fourth reactant lumens. The fourth reaction lumen contains a dry reactant of a solidified antigen, a solidified antibody, or a reactant corresponding to another target. Each fourth reactant lumen contains a label of the antigen, a label of the antibody, or a dry reactant of a matching reactant corresponding to the other target. The fourth reaction lumen is located at the axis of the fourth reagent tube body, and the two fourth reactant lumens are symmetrically mirrored on the front and rear sides of the fourth reaction lumen, and the upper surfaces of the fourth reaction lumen are flush with the upper surfaces of the two fourth reactant lumens.

[0026] The cross-section of the fourth reagent tube is rhomboid, the cross-section of the fourth reaction tube is circular, the cross-section of the fourth reactant tube is a triangle with an arc angle, and one side of the triangle with an arc angle is an arc concave surface. The arc concave surface of the fourth reactant tube faces the fourth reaction tube.

[0027] The length of the hollowed-out portion is 12mm~17mm, and the width is 9mm~11mm.

[0028] Furthermore, the reagent tube includes a fifth reagent tube body, a fifth reactant lumen, and a plurality of fifth reaction lumens. The fifth reactant lumen is used to contain whole blood, and the plurality of fifth reaction lumens are respectively used to contain hemoglobin, red blood cells, white blood cells, and platelet processing solutions. The fifth reactant lumen is located at the axis of the fifth reagent tube body, and the plurality of fifth reaction lumens are distributed in a ring around the outer periphery of the fifth reactant lumen, with the fifth reactant lumen flush with the upper end face of the plurality of fifth reaction lumens.

[0029] The cross-section of the fifth reagent tube is circular or elliptical, and the cross-section of the fifth reactant lumen and several fifth reaction lumens is circular; the diameter of the fifth reagent tube is 12~30mm; the diameter of the fifth reactant lumen is 3mm~6mm; and the diameter of the fifth reaction lumen is 2~4mm.

[0030] The length of the hollowed-out portion is 14mm~34mm, and the width is 14mm~34mm.

[0031] According to another aspect of the present invention, a method of using an integrated reagent tube tray includes the following steps:

[0032] S1, the integrated reagent tube tray is stored in the instrument. The integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket in the groove, and the automatic gripping device grabs the reagent tube.

[0033] S2, the instrument's clamping and shearing devices detach from the connecting beams on both sides of the reagent tube, and the automatic gripping device grips the reagent tube and loads it onto the detection reaction track;

[0034] S3, remove the sealing film from the reagent tube;

[0035] S4. Add purified water or appropriate buffer solution to the lumen of the reactant tube. The lumen of the reactant tube contains the dry reactant of the lyophilized antigen marker, antibody marker or other matching reactant corresponding to the target substance. Dissolve the lyophilized antigen marker, antibody marker or other matching reactant corresponding to the target substance in the lumen of the reactant tube with purified water or appropriate buffer solution.

[0036] S5, add the sample into the lumen of the reactant tube and mix well;

[0037] S6, transferring the mixed reactants from the reactant lumen to the reaction lumen;

[0038] S7, react until the specified time;

[0039] S8, perform pulsed continuous washing of the target reaction chamber to thoroughly remove unreacted components;

[0040] S9, add a specified volume of reactant for light information formation into the washed target reaction cavity, and react at a specified temperature for a specified time;

[0041] S10, directly detects the optical information value in the reaction cavity at the upper port of the reaction cavity;

[0042] S11. Based on the measured light information value, calculate the concentration or qualitative judgment value of the target substance in the target reaction cavity using the established standard curve, and report the detection results.

[0043] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0044] Firstly, the support, several reagent tubes, and connecting beams are integrally molded, eliminating the need for separate tube organizers, reagent storage modules, and reagent transfer devices found in traditional solutions. This significantly simplifies the overall structure, reduces the instrument's footprint, and lowers the high failure rate caused by multi-module assembly. The reagent tubes are detachably distributed on the support via the connecting beams, with each tube having a corresponding cutout. This provides operating space for the automatic gripping device and allows for optimization of the reagent tube's shape, avoiding time-consuming manual placement. The standardized support structure enables the reagent tubes to be used with various detection instruments, including colorimetric, fluorescence, luminescence, and imaging instruments, breaking down the incompatibility barriers between different platform containers and reducing detection costs. The reagent tubes contain built-in reaction lumens and reactant lumens. The reactant lumens can store dry or lyophilized reactants, such as antigen markers and antibody markers. This instrument eliminates the need for cold chain storage and transportation of PCR reagent components, avoiding the cold chain costs associated with liquid reagents and improving reagent stability. It also simplifies the instrument structure by eliminating the frequent cleaning of sampling needles required in traditional methods. The integrated reagent tube tray is compatible with colorimetric, fluorescence, luminescence, and imaging instruments, and multiple reagent tubes are integrated into the same support, allowing for flexible access for single or multiple tests to meet high-throughput detection needs. The sealing membrane provides independent sealing for each reagent tube lumen, ensuring reagent stability and preventing cross-contamination, further supporting automated high-throughput operations. The reaction lumen and reactant lumen are integrated into the same reagent tube, with a flush upper surface for easy overall sealing. Subsequent detections do not require separate reagent and container additions, simplifying the sample addition, reaction, and detection process, reducing manual intervention, and ensuring stable detection conditions.

[0045] Secondly, the precise fit between the positioning posts and positioning slots during stacking prevents the integrated reagent tube trays from shifting or tipping, enabling multi-layer dense storage. Compared to traditional scattered or simple stacking methods, this significantly saves storage space, such as in laboratory refrigerators and instrument storage compartments. In automated processes such as reagent manufacturing and in-instrument transfer, the positioning structure prevents relative sliding of the trays during stacked retrieval or movement, ensuring accurate positioning of the supports and reagent tubes. This avoids misalignment that could cause the automatic gripping device to fail to align with the reagent tubes, thus improving the reliability of automated operation.

[0046] Third, the groove serves as an instrument clamping device, such as a robotic arm, providing a clear and stable clamping point. This avoids the problems of slippage and clamping deviation that are common with traditional smooth supports, ensuring that the integrated reagent tube tray is accurately positioned during the process of "transferring from the storage position to the designated detection position," and reducing the risk of reagent tube damage or process interruption due to unstable clamping.

[0047] Fourth, each reagent tube is symmetrically equipped with a connecting beam on both sides of its bottom. This more evenly distributes the weight of the reagent tube and external forces, such as storage stacking pressure and vibration during automated transfer, preventing the reagent tube from tilting, falling off, or deforming its cavity. It is especially suitable for the stable storage of dry reactants, preventing reagent loss or performance changes caused by tube shaking. It also meets the pressure requirements for heat sealing the upper port of the reagent tube. The symmetrical layout allows the shearing device to apply force simultaneously from both sides of the reagent tube, ensuring balanced force when cutting the connecting beam. This prevents the reagent tube from shifting or flipping due to force on one side, ensuring that the reagent tube remains stable after separation and can be accurately picked up by the automatic gripping device without secondary adjustment. The connecting beam adopts a transverse strip design, with its extension and separation direction perpendicular to the gripping direction of the reagent tube. The cross-section of the connecting beam can adopt various shapes such as triangle, rhombus, trapezoid, triangular groove, trapezoidal groove, or cylinder, which can be flexibly selected according to the actual application scenario to meet the performance requirements of stability and easy access.

[0048] Fifth, the sixth reactant lumen is configured as a sample processing tube for centrifuging whole blood samples to convert them into plasma or serum samples for testing. This will greatly facilitate clinical testing, eliminate interference from particulate components such as cells in whole blood and peripheral blood samples, and improve the accuracy and reliability of test results. Attached Figure Description

[0049] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0050] Figure 1 This is a schematic diagram of the structure of Embodiment 1 in this invention;

[0051] Figure 2 This is a schematic diagram of the structure of Embodiment 2 in this invention;

[0052] Figure 3 This is a schematic diagram of the structure of Embodiment 3 in this invention;

[0053] Figure 4 This is a schematic diagram of the structure of Embodiment 4 in this invention;

[0054] Figure 5 This is a schematic diagram of the structure of Embodiment 5 in this invention;

[0055] Figure 6 This is a schematic diagram of the side structure of the integrated reagent tube tray of the present invention;

[0056] Figure 7This is a schematic cross-sectional view of the connecting beam in this invention;

[0057] In the diagram: Support-1, Hollowed-out part-11, Groove-12, Positioning post-13, Positioning slot-14, Reagent tube-2, First reagent tube body-2a, First reaction cavity-21a, First reactant cavity-22a, Second reagent tube body-2b, Second reaction cavity-21b, Second reactant cavity-22b, Sixth reactant cavity-23b, Third reagent tube body-2c, Third reaction cavity-21c, Third reactant cavity-22c, Fourth reagent tube body-2d, Fourth reaction cavity-21d, Fourth reactant cavity-22d, Fifth reagent tube body-2e, Fifth reaction cavity-21e, Fifth reactant cavity-22e, Connecting beam-3, Triangular connecting beam-3a, Triangular groove connecting beam-3b, Trapezoidal connecting beam-3c, Trapezoidal groove connecting beam-3d, Elliptical connecting beam-3e, Rhomboid connecting beam-3f. Detailed Implementation

[0058] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are for illustrative purposes only and do not limit the scope of the invention. Similarly, the following embodiments are only some, not all, embodiments of the present invention, and all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0059] like Figures 1 to 7 As shown, this solution provides an integrated reagent tray and its usage method. It is suitable for qualitative and quantitative analysis and detection of multiple targets in biological liquid samples using various methods such as colorimetry, fluorescence, luminescence, and imaging, which rely on changes in light signals.

[0060] Please see Figures 1 to 7 According to one aspect of the present invention, an integrated reagent tube tray is adapted to colorimetric instruments, fluorescence instruments, luminescence instruments, and imaging instruments. Specifically, colorimetric instruments can be biochemical analyzers, fluorescence instruments can be fluorescence immunoassay analyzers, real-time quantitative PCR instruments, luminescence instruments can be chemiluminescence immunoassay analyzers, and imaging instruments can be colloidal gold readers, digital imaging analyzers, and fully automated cell morphology analyzers. The aforementioned instruments are all direct applications of existing technologies, and their principles will not be elaborated here. The integrated reagent tube tray of the present invention is adapted to the aforementioned automated instruments. The reagent tubes are independently packaged and can be used for single or multiple tests, greatly simplifying the separate handling and loading of reaction containers and reagents in existing technologies, and eliminating waste caused by using the entire tray.

[0061] The present invention specifically includes: a support 1 and a plurality of reagent tubes 2. The support 1 is 60-150 mm long, 50-120 mm wide, and 2-6 mm high in the transverse direction. The support 1 has a plurality of hollow portions 11 at equal intervals. The reagent tubes 2 are detachably disposed in the hollow portions 11, with each hollow portion 11 corresponding to a plurality of reagent tubes 2. A connecting beam 3 is provided on the bottom left and right sides of the reagent tube 2 extending toward the support 1, and the connecting beam 3 is used to support the reagent tube 2. The reagent tube 2 is provided with at least one reaction lumen for reaction detection and at least one reactant lumen for containing reactants. The reactant lumen can contain corresponding reactant components, such as antigen markers or antibody markers, or PCR detection fluorescent markers, primers, dNTPs, Tag enzymes, etc. The reactants contained in the reactant lumen are in a dry solid or liquid state, and the reactant lumen is also the location where each stage of primary reaction occurs.

[0062] Furthermore, each reagent tube 2 has a detachable sealing membrane (not shown) for shielding the reaction chamber and reactant chamber. After the corresponding reactant components are prepared and placed, the reaction chamber and reactant chamber are independently heat-sealed with a membrane on the upper end face of each reagent tube 2 to form an independently usable reagent tube 2. The support 1, several reagent tubes 2, and several connecting beams 3 are integrally formed. The reagent tube 2 is automatically sheared from the connecting beams 3 and separated from the support 1 by extraction, so as to realize single-sample single-test or single-sample multi-index test detection, obtain target information, and perform qualitative and quantitative analysis.

[0063] Preferably, the support 1 has several positioning posts 13 extending upwards from its corners, each positioning post 13 having a positioning groove 14 at its lower end. When the integrated reagent tube trays are stacked, the positioning posts 13 of the support 1 are inserted into the positioning grooves 14. The precise fit between the positioning posts 13 and the positioning grooves 14 during stacking prevents the integrated reagent tube trays from shifting or tipping over, enabling multi-layer dense storage. Compared to traditional scattered or simple stacking methods, this significantly saves storage space, such as in laboratory refrigerators, instrument refrigeration, and cold storage areas. In automated processes such as reagent manufacturing and instrument transfer, the positioning structure prevents relative sliding of the tube trays during stacking, ensuring the accurate positioning of the support 1 and reagent tubes 2, preventing the automatic gripping device from failing to align with the reagent tubes due to offset, and improving the reliability of automated operation.

[0064] Preferably, the outer periphery of the support 1 is provided with a plurality of grooves 12 at intervals. These grooves 12 provide clamping points for the clamping device of the integrated reagent tube tray for fully automated operation of reagent manufacturing equipment and testing instruments. The clamping device, by clamping the grooves 12, makes the support 1 more stable during movement, further enabling the integrated reagent tube tray to achieve fully automated operation. The grooves 12, acting as instrument clamping devices such as robotic arms, provide clear and stable clamping points, avoiding the slippage and clamping deviation problems of traditional smooth supports. This ensures the accurate positioning of the integrated reagent tube tray during the transfer from the storage position to the designated detection position, reducing the risk of damage to the reagent tube 2 or process interruption due to unstable clamping.

[0065] Preferably, each reagent tube 2 has a connecting beam 3 symmetrically arranged at intervals on the bottom of its left and right sides. The connecting beam 3 is in the shape of a horizontal strip, and the reagent tube 2 is connected to the support 1 through the connecting beam 3. The cross-section of the connecting beam 3 is triangular, rhomboid, trapezoidal, triangular groove, trapezoidal groove, or cylindrical. The length of the connecting beam 3 is 0.3mm to 3mm, and the width and height do not exceed 2mm. More preferably, the connecting beams 3 at the left and right ends of the reagent tube 2 are mirror symmetrical.

[0066] Preferably, the connecting beam 3 includes a triangular connecting beam 3a, a triangular groove connecting beam 3b, a trapezoidal connecting beam 3c, a trapezoidal groove connecting beam 3d, an elliptical connecting beam 3e, and a rhomboid connecting beam 3f.

[0067] Each reagent tube 2 has a connecting beam 3 symmetrically arranged on its left and right bottom sides. This design more evenly distributes the weight and external forces of the reagent tube 2, such as storage stacking pressure and vibration during automated transfer, preventing the reagent tube 2 from tilting, falling off, or deforming its cavity. It is particularly suitable for the stable storage of dry reactants, preventing reagent loss or performance changes caused by tube shaking. It also meets the pressure requirements for heat sealing the upper port of the reagent tube 2. The symmetrical layout allows the shearing device to apply force simultaneously from both sides of the reagent tube 2, ensuring balanced force when cutting the connecting beam 3. This prevents the reagent tube 2 from shifting or flipping due to force on one side, ensuring that the reagent tube 2 remains stable after separation and can be accurately picked up by the automatic gripping device without secondary adjustment. The connecting beam 3 adopts a transverse strip design, with its extension and separation direction perpendicular to the gripping direction of the reagent tube. The cross-section of the connecting beam can adopt various shapes such as triangle, rhombus, trapezoid, triangular groove, trapezoidal groove, or cylinder, which can be flexibly selected according to the actual application scenario to meet performance requirements such as stability and ease of handling.

[0068] Example 1:

[0069] Please see Figure 1The reagent tube 2 includes a first reagent tube body 2a, a first reaction lumen 21a, and a first reactant lumen 22a. The first reagent tube body 2a is injection molded from white, opaque PS material to meet the requirements of the luminescent immunoassay method, effectively shielding against ambient stray light interference and ensuring the accuracy of luminescent signal detection. The support 1 is 110mm long, 100mm wide, and 4mm high. In this embodiment, the connecting beam 3 is a triangular connecting beam 3a with a lateral length of 1mm and a width and height of ≤1.5mm. This embodiment is used for heterogeneous or homogeneous luminescent immunoassay. The first reaction lumen 21a contains a dry reactant of a solidified antigen, a solidified antibody, or other target substance corresponding to a target substance. The first reactant lumen 22a contains a dry reactant of a marker for an antigen, a marker for an antibody, or other target substance corresponding to a target substance. The first reaction lumen 21a and the first reactant lumen 22a are arranged at an interval between the front and rear of the first reagent tube 2a, and the upper end face of the first reaction lumen 21a is flush with the upper end face of the first reactant lumen 22a. Furthermore, the bottom of the first reaction lumen 21a may be formed with a convex and concave rotating body to increase the internal surface area.

[0070] The first reagent tube 2a has an elliptical cross-section, the first reaction cavity 21a has a circular cross-section, and the first reactant cavity 22a has a crescent-shaped cross-section. The concave surface of the first reactant cavity 22a faces the first reaction cavity 21a. This makes full use of the limited space of the first reagent tube 2a. Preferably, the bottom of the first reactant cavity 22a has a narrowed arc-shaped structure to facilitate the use of liquid reactants.

[0071] The major semi-axis a of the ellipse of the first reagent tube body 2a is 4.5mm~7.5mm, preferably 5mm. The minor semi-axis b is 3.6mm~4.4mm, preferably 4mm. The centrifugation rate e is 0.6~0.88, preferably 0.6; the diameter of the first reaction cavity 21a is 3~9mm, preferably 4.6mm. The length of the hollow portion 11 is 12mm~17mm, and the width is 9mm~11mm, preferably 13mm and 10mm. The hollow portion 11 provides ample operating space for the automatic gripping device while ensuring easy cutting of the connecting beam 3 and convenient separation of the reagent tube, making it suitable for high-throughput luminescence detection scenarios.

[0072] Example 1 describes a method for using an integrated reagent tube tray for heterogeneous luminescent immunoassay, comprising the following steps:

[0073] S1, in the integrated reagent tube storage position stored in the instrument, the integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket 1 through the groove 12, and the automatic gripping device grips the first reagent tube body 2a.

[0074] S2, the clamping device and shearing device of the instrument disconnect the triangular connecting beams 3a on both sides of the first reagent tube 2a, and the first reagent tube 2a is grabbed by the automatic gripping device and loaded onto the detection reaction track.

[0075] S3, remove the sealing film from the first reagent tube 2a;

[0076] S4, add purified water or appropriate buffer solution into the first reactant lumen 22a. The first reactant lumen 22a contains the dry reactant of the lyophilized antigen marker, antibody marker or other matching reactant corresponding to the target substance. Dissolve the lyophilized antigen marker, antibody marker or other matching reactant corresponding to the target substance in the first reactant lumen 22a with purified water or appropriate buffer solution.

[0077] S5, add the sample into the lumen 22a of the first reactant and mix well;

[0078] S6, the mixed reactants are transferred from the first reactant cavity 22a to the first reaction cavity 21a;

[0079] S7, react until the specified time;

[0080] S8, perform pulsed continuous washing on the target first reaction chamber 21a to fully remove unreacted components;

[0081] S9, add a specified volume of reactant for the formation of light information (referring to the light absorption, light emission or fluorescence) into the washed target first reaction cavity 21a, and react for a specified time at a specified temperature.

[0082] S10, the optical information value in the first reaction cavity 21a is directly detected at the upper port of the first reaction cavity 21a;

[0083] S11, based on the measured light information value, calculate the concentration or qualitative judgment value of the target substance in the first reaction cavity 21a of the target using the established standard curve, and report the detection results.

[0084] Example 2:

[0085] Please see Figure 2 The difference between this embodiment and Embodiment 1 is that:

[0086] The reagent tube 2 includes a second reagent tube body 2b, a second reaction cavity 21b, a second reactant cavity 22b, and a sixth reactant cavity 23b. The second reagent tube body 2b is injection molded from white PS material and is used for homogeneous or heterogeneous luminescence detection. It can effectively shield ambient stray light interference and ensure the accuracy of luminescence signal detection. The support 1 is 110mm long, 100mm wide, and 4mm high. In this embodiment, the connecting beam 3 is a trapezoidal connecting beam 3c. The lateral length of the trapezoidal connecting beam 3c is 1mm, and its width and height are both ≤1.5mm.

[0087] The second reaction lumen 21b contains a dry reactant of a solidified antigen, a solidified antibody, or other target substance corresponding to a target substance. The second reactant lumen 22b contains a dry reactant of a marker for an antigen, a marker for an antibody, or other target substance corresponding to a target substance. The sixth reactant lumen 23b is configured as a sample processing tube for centrifuging whole blood samples or other samples containing particulate matter to convert them into plasma or serum samples for use as detection samples. The sixth reactant lumen 23b, the second reactant lumen 22b, and the second reaction lumen 21b are arranged sequentially from front to back in the second reagent tube body 2b, and the sixth reactant lumen 23b and the second reactant lumen 22b are flush with the upper end face of the second reaction lumen 21b. Furthermore, the bottom of the second reaction lumen 21b used for heterogeneous immunoassay can be formed into a convex and concave rotating body to increase the internal surface area.

[0088] The second reagent tube 2b has an elliptical cross-section, the second reaction cavity 21b has a circular cross-section, and the sixth reactant cavity 23b and the second reactant cavity 22b both have crescent-shaped cross-sections. The crescent-shaped concave surfaces of the sixth reactant cavity 23b and the second reactant cavity 22b overlap and interlock on one side of the second reaction cavity 21b, with both crescent-shaped concave surfaces facing the second reagent tube 2b, thus making full use of the limited space of the second reagent tube 2b. Preferably, the bottoms of the sixth reactant cavity 23b and the second reactant cavity 22b have a narrowed arc-shaped structure to facilitate the handling of liquid reactants.

[0089] The major semi-axis a of the ellipse of the second reagent tube 2b is 4.5mm to 7.5mm, preferably 7mm. The minor semi-axis b is 3.6mm to 4.4mm, preferably 4mm. The centrifugation rate e is 0.6 to 0.88, preferably 0.746. The diameter of the second reaction chamber 21b is 3 to 9mm, preferably 4.6mm; the length of the hollow portion 11 is 12mm to 17mm, and the width is 9mm to 11mm. Preferably, the length of the hollow portion 11 is 16mm and the width is 10mm. This provides operating space for the gripping device inside the instrument to grip the second reagent tube 2b.

[0090] Example 2 describes a method for using an integrated reagent tube tray for homogeneous luminescent immunoassay, comprising the following steps:

[0091] S1, in the integrated reagent tube storage position stored in the instrument, the integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket 1 through the groove 12, and the automatic gripping device grips the second reagent tube body 2b.

[0092] S2, the clamping device and shearing device of the instrument disconnect the trapezoidal connecting beams 3c on both sides of the second reagent tube 2b, and the second reagent tube 2b is grabbed by the automatic gripping device and loaded onto the detection reaction track.

[0093] S3, remove the sealing film on the second reagent tube 2b;

[0094] S4. Add purified water or appropriate buffer solution to the second reaction chamber 21b and the second reactant chamber 22b to dissolve the dry reactant.

[0095] S5, add whole blood or a sample containing particulate matter into lumen 23b of the sixth reactant tube, hold it with the robotic arm on the reaction track and centrifuge at 400~4000 rpm for 10~30s;

[0096] S6, transfer the plasma in the sixth reactant lumen 23b to the second reactant lumen 22b and mix well;

[0097] S7, transfer the reaction mixture in the second reactant chamber 22b to the second reaction chamber 21b and mix well;

[0098] S8, react until the specified time;

[0099] S9, excite and detect the optical information value in the second reaction cavity 21b from the upper port of the second reaction cavity 21b;

[0100] S10, based on the measured light information value, calculate the concentration or qualitative judgment value of the target substance in the second reaction cavity 21b of the target using the established standard curve, and report the detection results.

[0101] Example 3:

[0102] Please see Figure 1 The difference between this embodiment and Embodiment 1 is that:

[0103] The reagent tube 2 includes a first reagent tube body 2a, a first reaction lumen 21a, and a first reactant lumen 22a. The first reagent tube body 2a is injection molded from natural-colored, light-transmitting PP material to ensure that the fluorescence signal penetrates without attenuation, adapting to the side-position detection optical path of the fluorescence instrument and ensuring the accuracy of fluorescence signal detection. The support 1 is 110mm long, 100mm wide, and 4mm high. In this embodiment, the connecting beam 3 is a triangular connecting beam 3a, with a lateral length of 1mm and a width and height of ≤1.5mm. This embodiment is used for fluorescence detection. The first reaction lumen 21a contains Tag enzyme dry reactants, and the first reactant lumen 22a contains PCR detection fluorescent markers, primers, and dNTP dry reactants. The first reaction lumen 21a and the first reactant lumen 22a are spaced apart in front of and behind the first reagent tube body 2a, and the upper surfaces of the first reaction lumen 21a and the first reactant lumen 22a are flush. Furthermore, the bottom of the first reaction lumen 21a has a flat bottom structure.

[0104] The first reagent tube 2a has an elliptical cross-section, the first reaction cavity 21a has a circular cross-section, and the first reactant cavity 22a has a crescent-shaped cross-section. The concave surface of the first reactant cavity 22a faces the first reaction cavity 21a. This makes full use of the limited space of the first reagent tube 2a. Preferably, the bottom of the first reactant cavity 22a has a narrowed arc-shaped structure to facilitate the use of liquid reactants.

[0105] The major semi-axis a of the ellipse of the first reagent tube body 2a is 4.5mm~7.5mm, preferably 5mm. The minor semi-axis b is 3.6mm~4.4mm, preferably 4mm. The centrifugation rate e is 0.6~0.88, preferably 0.6; the diameter of the first reaction cavity 21a is 3~9mm, preferably 4.6mm. The length of the hollow portion 11 is 12mm~17mm, and the width is 9mm~11mm, preferably 13mm and 10mm. The hollow portion 11 provides ample operating space for the automatic gripping device while ensuring easy cutting of the connecting beam 3 and convenient separation of the reagent tube, making it suitable for high-throughput luminescence detection scenarios.

[0106] Example 3 describes a method for using an integrated reagent tube tray for fluorescence detection, comprising the following steps:

[0107] S1, in the integrated reagent tube storage position stored in the instrument, the integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket 1 through the groove 12, and the automatic gripping device grips the first reagent tube body 2a.

[0108] S2, the clamping device and shearing device of the instrument disconnect the trapezoidal connecting beams 3c on both sides of the first reagent tube 2a, and the first reagent tube 2a is grabbed by the automatic gripping device and loaded onto the detection reaction track.

[0109] S3, remove the sealing film from the first reagent tube 2a;

[0110] S4, add purified water or corresponding buffer solution to the first reaction chamber 21a and the first reactant chamber 22a respectively. The first reaction chamber 21a and the first reactant chamber 22a respectively contain lyophilized target analyte detection reactants, and dissolve the dry reactants.

[0111] S5, add the sample into the lumen 22a of the first reactant and mix well;

[0112] S6, in step S5, the mixed reactants in the first reactant cavity 22a are transferred to the first reaction cavity 21a;

[0113] S7, cover the upper port of the first reaction chamber 21a with a sleeve;

[0114] S8, according to the specified temperature change program of denaturation (95℃) - annealing (60℃) - extension (72℃) for 35-45 cycles;

[0115] S9, detect the fluorescence value of the reaction product in the first reaction chamber 21a from the arc side of the outer wall of the first reaction chamber 21a: plot the amplification curve of the reactant product;

[0116] S10. Based on the measured fluorescence intensity value, calculate the concentration of the target substance in the second reaction lumen 21b using the internal reference ΔΔCtMethod or the double standard curve method, and report the detection results.

[0117] Example 4:

[0118] Please see Figure 3 The difference between this embodiment and Embodiment 1 is that:

[0119] The reagent tube 2 includes a third reagent tube body 2c, a third reaction chamber 21c, and at least two third reactant chambers 22c. The third reagent tube body 2c is injection molded from natural-colored, translucent PS material and is used for light absorption detection. The support 1 is 110mm long, 100mm wide, and 4mm high. In this embodiment, the connecting beam 3 is a triangular connecting beam 3a. The lateral length of the triangular connecting beam 3a is 1mm, and its width and height are both ≤1.5mm. The bottoms of the two third reactant chambers 22c have a narrowing arc-shaped structure, which facilitates the use of liquid reactants. The bottom of the third reaction chamber 21c has a flat mirror structure, ensuring that when the instrument light source is vertically projected, the light path penetrates the liquid sample vertically, avoiding absorbance deviations caused by chamber offset.

[0120] The third reaction lumen 21c contains a dry reaction mixture of a solidified antigen, a solidified antibody, or other target substance corresponding to a target substance. Each third reaction lumen 22c contains a label of the antigen, a label of the antibody, or a dry reaction mixture of a matching reactant corresponding to the aforementioned target substance. The third reaction lumen 21c is located at the axis of the third reagent tube 2c. The two third reaction lumen 22cs are symmetrically mirrored on the front and rear sides of the third reaction lumen 21c, and the upper end faces of the third reaction lumen 21c are flush with the upper end faces of the two third reaction lumen 22cs. The cross-section of the third reagent tube 2c is elliptical, the cross-section of the third reaction lumen 21c is circular, and the cross-section of the third reaction lumen 22c is crescent-shaped. The concave surface of the third reaction lumen 22c faces the third reaction lumen 21c.

[0121] The major semi-axis a of the ellipse of the third reagent tube 2c is 4.5mm~7.5mm, preferably 6.5mm. The minor semi-axis b is 3.6mm~4.4mm, preferably 4mm. The centrifugal rate e is 0.6~0.88; preferably 0.788. The length of the hollow portion 11 is 12mm~17mm, and the width is 9mm~11mm. Preferably, the length of the hollow portion 11 is 13mm, and the width is 10mm.

[0122] Example 4 describes a method for using an integrated reagent tube tray for colorimetric absorbance detection, comprising the following steps:

[0123] S1, the integrated reagent tube tray is stored in the instrument. The integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket 1 through the groove 12, and the automatic gripping device grabs the third reagent tube 2c.

[0124] S2, the clamping device and shearing device of the instrument disconnect the triangular connecting beams 3a on both sides of the third reagent tube 2c, and the third reagent tube 2c is grabbed by the automatic gripping device and loaded onto the detection reaction track.

[0125] S3, remove the sealing film on the third reagent tube 2c;

[0126] S4. Add purified water or appropriate buffer solution to the third reactant lumen 22c. The third reactant lumen 22c contains the dry reactant of the antigen corresponding to the lyophilized target substance, the antibody corresponding to the target substance, or the matching reactant of the other target substances mentioned above. Dissolve the dry reactant.

[0127] S5, add the sample into one of the third reactant lumens 22c and mix well;

[0128] S6, the mixed reactants from step S5 are transferred to another third reactant lumen 22c;

[0129] S7, react until the specified time;

[0130] S8, the mixed reactants from step S6 are transferred to the third reaction chamber 21c;

[0131] S9, react until the specified time;

[0132] S10, the instrument light source is located above the opening of the third reaction chamber 21c, and a light source of a specified wavelength is vertically projected onto the opening of the third reaction chamber 21c, and the absorbance value is directly detected from outside the bottom wall of the third reaction chamber 21c.

[0133] S11. Based on the measured absorbance value, calculate the concentration of the target substance in the third reaction chamber 21c using the established standard curve, and report the detection results.

[0134] Example 5:

[0135] Please see Figure 4 The difference between this embodiment and Embodiment 1 is that:

[0136] The reagent tube 2 includes a fourth reagent tube body 2d, a fourth reaction lumen 21d, and at least two fourth reactant lumen 22d. The fourth reagent tube body 2d is injection molded from natural-colored translucent PS material and is used for enzyme-linked immunosorbent assay (ELISA). The support 1 is 110mm long, 100mm wide, and 4mm high. In this embodiment, the connecting beam 3 is a trapezoidal groove-shaped connecting beam 3d. The lateral length of the trapezoidal groove-shaped connecting beam 3d is 1mm, and its width and height are both ≤1.5mm. The bottoms of the two fourth reactant lumen 22d have a narrowing arc-shaped structure to facilitate the removal of liquid reactants. The bottom of the fourth reaction lumen 21d has a flat mirror structure. The fourth reaction lumen 21d contains a dry reaction product of a solidified antigen, a solidified antibody, or other target substance corresponding to a target substance. Each fourth reaction lumen 22d contains a label of the antigen, a label of the antibody, or a dry reaction product of a matching reaction product corresponding to the above-mentioned target substance. The fourth reaction lumen 21d is located at the axis of the fourth reagent tube 2d. The two fourth reaction lumens 22d are symmetrically mirrored on the front and rear sides of the fourth reaction lumen 21d, and the upper end surfaces of the fourth reaction lumen 21d and the two fourth reaction lumens 22d are flush.

[0137] The cross-section of the fourth reagent tube 2d is rhomboid. The rhomboid geometry provides bidirectional force balance, preventing deformation due to external forces during automated clamping and shearing operations, such as the shearing separation of the trapezoidal groove connecting beam 3d. The cross-section of the fourth reaction tube 21d is circular. The inner wall of the circular cross-section is smooth and without sharp edges. When the instrument light source shines vertically, the light can penetrate the liquid sample inside the tube evenly, avoiding uneven light refraction and reflection caused by the tube's sharp edges. The cross-section of the fourth reactant tube 22d is a triangle with curved angles, and one side of this triangle is a concave arc. The concave arc of the fourth reactant tube 22d faces the fourth reaction tube 21d, making full use of the limited space of the fourth reagent tube 2d.

[0138] Traditional triangular cross-section tubes with right angles or sharp corners are prone to multiple problems in practical applications, while the optimized curved corners completely avoid these defects. Specifically: the reagent tube tray is manufactured using a one-piece injection molding process. Sharp corners are prone to stress concentration after molding, which may lead to micro-cracks or plastic residues at the corners of the tube. In particular, sharp corners are prone to reactant residues due to the wetting effect of liquid and tube wall molecules. The curved surface design can disperse injection stress, prevent residues from falling off and contaminating the dry reactants in the tube, and ensure the accuracy of the test results. It can also prevent dead corners where lyophilized dry reactants accumulate due to transport vibrations.

[0139] The length of the cutout portion 11 is 12mm~17mm, and the width is 9mm~11mm. Preferably, the length of the cutout portion 11 is 13mm, and the width is 10mm.

[0140] Example 5 describes a method for using an integrated reagent tube tray for enzyme-linked immunosorbent assay (ELISA), comprising the following steps:

[0141] S1, the integrated reagent tube tray is stored in the instrument. The integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket 1 through the groove 12, and the automatic gripping device grips the fourth reagent tube 2d.

[0142] S2, the clamping device and shearing device of the instrument disconnect the trapezoidal groove connecting beams 3d on both sides of the fourth reagent tube 2d, and the automatic gripping device grips the fourth reagent tube 2d and loads the fourth reagent tube 2d onto the detection reaction track.

[0143] S3, remove the sealing film on the fourth reagent tube 2d;

[0144] S4, add purified water or appropriate buffer solution to one of the fourth reactant lumen 22d. The fourth reactant lumen 22d contains the lyophilized antigen marker or antibody marker corresponding to the target substance, and dissolve the dry reactant.

[0145] S5, add the sample into the lumen 22d of the fourth reactant tube and mix well;

[0146] S6, the mixed reactants from step S5 are transferred to the fourth reaction chamber 21d;

[0147] S7, react until the specified time;

[0148] S8, perform pulsed continuous washing on the target fourth reaction chamber 21d to fully remove unreacted components;

[0149] S9, add a specified volume of substrate reactant into the washed target fourth reaction chamber 21d, and react at a specified temperature for a specified time;

[0150] S10, add the specified volume of stop solution into the washed target fourth reaction chamber 21d to terminate the reaction;

[0151] S11, the instrument light source is located above the opening of the fourth reaction chamber 21d, and a light source of a specified wavelength is vertically projected onto the opening of the fourth reaction chamber 21d, and the absorbance value is directly detected from outside the bottom wall of the fourth reaction chamber 21d.

[0152] S12, based on the measured absorbance value, calculate the concentration of the target substance in the fourth reaction chamber 21d using the established standard curve, and report the detection results.

[0153] Example 6:

[0154] Please see Figure 5 The difference between this embodiment and Embodiment 1 is that:

[0155] The reagent tube 2 includes a fifth reagent tube body 2e, a fifth reactant lumen 22e, and several fifth reaction lumens 21e. In this embodiment, there are four fifth reaction lumens 21e. The fifth reagent tube body 2e is injection molded from natural-colored translucent PS material and is used for cell imaging analysis and hemoglobin colorimetric detection in blood cell analysis. The support 1 is 110mm long, 100mm wide, and 4mm high. In this embodiment, the connecting beam 3 is a triangular groove-shaped connecting beam 3b. The lateral length of the triangular groove-shaped connecting beam 3b is 1mm, and its width and height are both ≤1.5mm. The fifth reagent tube body 2e has a flat-bottomed mirror structure. The fifth reaction lumen 21e is a container used for reaction, analysis, detection, and imaging.

[0156] The fifth reactant lumen 22e is used to contain whole blood, specifically for dispensing and diluting whole blood. Several fifth reactant lumens 21e are used to contain hemoglobin, red blood cells, white blood cells, and platelet processing solutions, specifically for dispensing and diluting hemoglobin for colorimetric detection and for imaging red blood cells, white blood cells, and platelets. The fifth reactant lumen 22e is located at the axis of the five-reagent tube body 2e. Several fifth reactant lumens 21e are arranged in a ring around the outer periphery of the fifth reactant lumen 22e, with each lumen 21e evenly distributed in a ring, ensuring consistent optical path angles during imaging and avoiding morphological recognition or counting errors caused by positional deviations. Furthermore, the upper surfaces of the fifth reactant lumen 22e are flush with the upper surfaces of the several fifth reactant lumens 21e. This embodiment can simultaneously complete "hemoglobin colorimetry + morphological imaging and classification of red blood cells, white blood cells, and platelets," eliminating the need for multiple independent test tubes for separate operations. Traditional blood routine tests require 4-5 test tubes; now, only one test tube is needed, shortening the testing process.

[0157] The fifth reagent tube 2e is circular or elliptical, and the cross-section of the fifth reactant lumen 22e and several fifth reaction lumens 21e are all circular; the diameter of the fifth reagent tube 2e is 12~30mm, the diameter of the fifth reactant lumen 22e is 3mm~6mm, the diameter of the fifth reaction lumen 21e is 2~4mm, and the length and width of the hollow part 11 are 14mm~34mm.

[0158] Example 6 describes a method for using an integrated reagent tube tray, comprising the following steps:

[0159] S1, the integrated reagent tube tray is stored in the instrument. The integrated reagent tube tray is moved to the designated position by the clamping device of the instrument clamping bracket 1 through the groove 12, and the automatic gripping device grips the fifth reagent tube 2e.

[0160] S2, the clamping device and shearing device of the instrument disconnect the triangular hollow connecting beams 3b on both sides of the fifth reagent tube 2e, and the fifth reagent tube 2e is grabbed by the automatic gripping device and loaded onto the detection reaction track.

[0161] S3, remove the sealing film from the fifth reagent tube 2e;

[0162] S4, add the specified volume of whole blood into the lumen 22e of the fifth reactant and mix well;

[0163] S5, take out the specified volume of diluted whole blood in sequence and add it into each fifth reaction tube 21e, and mix well;

[0164] S6. Use a wavelength of 540nm to measure the hemoglobin in one of the 21e lumen of the fifth reaction tube, record its absorbance value, and calculate the hemoglobin concentration from the calibration curve.

[0165] S7. Photographs of red blood cells, white blood cells, and platelets in three tubes of the other fifth reaction lumen 21 were taken at different wavelengths. The morphology, quantity, and subtype were recorded and analyzed for reporting.

[0166] S8 reports the test results based on the measurement results and in conjunction with the intelligent analysis system.

[0167] In this embodiment, the layout of the central fifth reactant lumen 22e and the annular fifth reactant lumen 21e can simultaneously complete the entire process of "dilution, sample addition, colorimetry, and imaging" in an automated manner, without the need for manual sample transfer, thus meeting the high-throughput testing needs of clinical blood routine tests.

[0168] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An integrated reagent tube tray, adaptable to colorimetric instruments, fluorescence instruments, luminescence instruments, and imaging instruments, characterized in that, include: A support (1) and several reagent tubes (2) are provided. The support (1) has several hollowed-out portions (11) spaced apart. The reagent tubes (2) are detachably disposed in the hollowed-out portions (11). Each hollowed-out portion (11) corresponds to one of the reagent tubes (2). A connecting beam (3) extends from the bottom left and right sides of the reagent tubes (2) toward the support (1). Each reagent tube (2) is provided with at least one reaction cavity for reaction detection and at least one reactant cavity for containing reactants. Each reagent tube (2) has an opening that is detachably provided with an independent sealing membrane for shielding the reaction cavity and the reactant cavity. The support (1), several reagent tubes (2) and several connecting beams (3) are integrally formed. The reagent tubes (2) are separated from the support (1) by automatic shearing extraction to achieve single-person single test or single-person multi-index test detection, obtain target information, and perform qualitative and quantitative analysis on it. The specific structure of the reagent tube (2) is as follows: the reagent tube (2) includes a first reagent tube body (2a), a first reaction lumen (21a), and a first reactant lumen (22a). The first reaction lumen (21a) contains a dry reactant of a solid-phase antigen, a solid-phase antibody, or other target substance. The first reactant lumen (22a) contains a label of the antigen, a label of the antibody, or a dry reactant of the other target substance. The first reaction lumen (21a) and the first reactant lumen (22a) are arranged at an interval between the first reagent tube body (2a) and the first reactant lumen (22a). The upper surface of the cavity (22a) is flush; the cross-section of the first reagent tube (2a) is elliptical, the cross-section of the first reaction cavity (21a) is circular, the cross-section of the first reactant cavity (22a) is crescent-shaped, and the concave surface of the first reactant cavity (22a) faces the first reaction cavity (21a); ​​the major semi-axis a of the ellipse of the first reagent tube (2a) is 4.5mm~7.5mm, the minor semi-axis b is 3.6mm~4.4mm, and the eccentricity e is 0.6~0.88; the diameter of the first reaction cavity (21a) is 3~9mm; the length of the hollow part (11) is 12mm~17mm, and the width is 9mm~11mm.

2. The integrated reagent tube tray as described in claim 1, characterized in that, The bracket (1) has several positioning posts (13) extending upward from its corners. Each positioning post (13) has a positioning groove (14) at its lower end. When the integrated reagent tube trays are stacked, the positioning posts (13) of the bracket (1) are inserted into the positioning grooves (14).

3. The integrated reagent tube tray as described in claim 1, characterized in that, The bracket (1) has several grooves (12) spaced apart on its outer periphery. The grooves (12) are used to provide clamping points for the clamping device of the reagent tube disk integrated in the fully automatic operation of reagent manufacturing equipment and testing instruments, so as to achieve precise docking and positioning.

4. The integrated reagent tube tray as described in claim 1, characterized in that, Each reagent tube (2) has a connecting beam (3) symmetrically arranged at intervals on the bottom of its left and right sides. The connecting beam (3) is in the shape of a horizontal strip. The reagent tube (2) is connected to the support (1) through the connecting beam (3). The cross-section of the connecting beam (3) is triangular, rhomboid, trapezoidal, triangular groove, trapezoidal groove or cylindrical. The length of the connecting beam (3) is 0.3mm~3mm, and the width and height are both no more than 2mm.

5. An integrated reagent tube tray as described in claim 1, characterized in that, The specific structure of the reagent tube (2) is replaced as follows: the reagent tube (2) includes a second reagent tube body (2b), a second reaction lumen (21b), a second reactant lumen (22b), and a sixth reactant lumen (23b). The second reaction lumen (21b) contains dry reactants of solidified antigens, solidified antibodies, or other target substances. The second reactant lumen (22b) contains dry reactants of antigen markers, antibody markers, or other target substances. The sixth reactant lumen (23b) is set as a sample processing tube for centrifuging whole blood samples to convert them into plasma or serum samples for use as detection samples. The sixth reactant lumen (23b), the second reactant lumen (22b), and the second reaction lumen (21b) are arranged sequentially from front to back in the second reagent tube body (2b), and the upper surfaces of the sixth reactant lumen (23b), the second reactant lumen (22b), and the second reaction lumen (21b) are flush. The cross-section of the second reagent tube (2b) is elliptical, the cross-section of the second reaction cavity (21b) is circular, and the cross-sections of the second reactant cavity (22b) and the sixth reactant cavity (23b) are both crescent-shaped. The crescent-shaped concave surfaces of the sixth reactant cavity (23b) and the second reactant cavity (22b) overlap and interlock on one side of the second reaction cavity (21b), and the crescent-shaped concave surfaces all face the second reagent tube (2b). The second reagent tube body (2b) has a major semi-axis a of 4.5mm to 7.5mm, a minor semi-axis b of 3.6mm to 4.4mm, and an eccentricity e of 0.6 to 0.88; the second reaction tube lumen (21b) has a diameter of 3 to 9mm. The length of the hollow part (11) is 12mm~17mm and the width is 9mm~11mm.

6. The integrated reagent tube tray as described in claim 1, characterized in that, The specific structure of the reagent tube (2) is replaced as follows: the reagent tube (2) includes a third reagent tube body (2c), a third reaction lumen (21c), and at least two third reactant lumens (22c). The third reaction lumen (21c) contains dry reactants of solidified antigens, solidified antibodies, or other target substances. Each third reactant lumen (22c) contains a label of the antigen, a label of the antibody, or a dry reactant of the matching reactant corresponding to the above-mentioned other target substances. The third reaction lumen (21c) is located at the axis of the third reagent tube body (2c). The two third reactant lumens (22c) are symmetrically mirrored on the front and rear sides of the third reaction lumen (21c), and the third reaction lumen (21c) is flush with the upper surface of the two third reactant lumens (22c). The cross-section of the third reagent tube (2c) is elliptical, the cross-section of the third reaction cavity (21c) is circular, the cross-section of the third reactant cavity (22c) is crescent-shaped, and the concave surface of the third reactant cavity (22c) faces the third reaction cavity (21c). The major semi-axis a of the ellipse of the third reagent tube (2c) is 4.5mm~7.5mm, the minor semi-axis b is 3.6mm~4.4mm, and the eccentricity e is 0.6~0.

88. The length of the hollow part (11) is 12mm~17mm and the width is 9mm~11mm.

7. The integrated reagent tube tray as described in claim 1, characterized in that, The specific structure of the reagent tube (2) is replaced as follows: the reagent tube (2) includes a fourth reagent tube body (2d), a fourth reaction lumen (21d), and at least two fourth reactant lumens (22d). The fourth reaction lumen (21d) contains dry reactants of solidified antigens, solidified antibodies, or other target substances. Each fourth reactant lumen (22d) contains a label of the antigen, a label of the antibody, or a dry reactant of the matching reactant corresponding to the above-mentioned other target substances. The fourth reaction lumen (21d) is located at the axis of the fourth reagent tube body (2d). The two fourth reactant lumens (22d) are symmetrically mirrored on the front and rear sides of the fourth reaction lumen (21d), and the fourth reaction lumen (21d) is flush with the upper surface of the two fourth reactant lumens (22d). The cross-section of the fourth reagent tube (2d) is rhomboid, the cross-section of the fourth reaction tube (21d) is circular, the cross-section of the fourth reactant tube (22d) is a triangle with an arc angle, and one side of the triangle with an arc angle is an arc concave surface. The arc concave surface of the fourth reactant tube (22d) faces the fourth reaction tube (21d). The length of the hollow part (11) is 12mm~17mm and the width is 9mm~11mm.

8. The integrated reagent tube tray as described in claim 1, characterized in that, The specific structure of the reagent tube (2) is replaced as follows: the reagent tube (2) includes a fifth reagent tube body (2e), a fifth reactant lumen (22e), and a plurality of fifth reaction lumens (21e). The fifth reactant lumen (22e) is used to contain whole blood, and the plurality of fifth reaction lumens (21e) are respectively used to contain hemoglobin, red blood cells, white blood cells, and platelet processing solutions. The fifth reactant lumen (22e) is located at the axis of the fifth reagent tube body (2e), and the plurality of fifth reaction lumens (21e) are distributed in a ring at intervals around the outer periphery of the fifth reactant lumen (22e), and the fifth reactant lumen (22e) is flush with the upper end face of the plurality of fifth reaction lumens (21e). The cross-section of the fifth reagent tube (2e) is circular or elliptical, and the cross-section of the fifth reactant lumen (22e) and several fifth reaction lumens (21e) is circular; the diameter of the fifth reagent tube (2e) is 12~30mm; the diameter of the fifth reactant lumen (22e) is 3mm~6mm; and the diameter of the fifth reaction lumen (21e) is 2~4mm. The length of the hollow part (11) is 14mm~34mm and the width is 14mm~34mm.

9. A method of using an integrated reagent tube tray according to claim 3, characterized in that, Includes the following steps: S1, in the integrated reagent tube storage position stored in the instrument, the integrated reagent tube tray is moved to the designated position by clamping the groove (12) of the support (1) through the clamping device of the instrument, and the automatic gripping device grabs the reagent tube (2). S2, the clamping device and shearing device of the instrument are separated from the connecting beams (3) on both sides of the reagent tube (2), and the reagent tube (2) is grabbed by the automatic gripping device and loaded onto the detection reaction track; S3, remove the sealing film from the reagent tube (2); S4. Add purified water or appropriate buffer solution to the lumen of the reactant tube. The lumen of the reactant tube contains the dry reactant of the lyophilized antigen marker, antibody marker or other matching reactant corresponding to the target substance. Dissolve the lyophilized antigen marker, antibody marker or other matching reactant corresponding to the target substance in the lumen of the reactant tube with purified water or appropriate buffer solution. S5, add the sample into the lumen of the reactant tube and mix well; S6, transferring the mixed reactants from the reactant lumen to the reaction lumen; S7, react until the specified time; S8, perform pulsed continuous washing of the target reaction chamber to thoroughly remove unreacted components; S9, add a specified volume of reactant for light information formation into the washed target reaction cavity, and react at a specified temperature for a specified time; S10, directly detects the optical information value in the reaction cavity at the upper port of the reaction cavity; S11. Based on the measured light information value, calculate the concentration or qualitative judgment value of the target substance in the target reaction cavity using the established standard curve, and report the detection results.

Images