Non-contact type biological particle processing equipment and biological particle processing equipment

Abstract

The present invention discloses a non-contact type biological particle processing device and a biological particle processing device. [Solution] The bioparticle processing device includes a droplet generation chamber, a working chamber connected to the droplet generation chamber, and a sorting chamber connected to the working chamber. The droplet generation chamber receives a first liquid, bioparticles located in the first liquid, and a second liquid insoluble in the first liquid. The droplet generation chamber causes the flow of the second liquid to cross the first liquid, forming bioparticle droplets that flow toward the working chamber after passing through the bioparticles and a portion of the first liquid surrounding them, and the bioparticles are cultured or detected using the first liquid.

Description

Technical Field

[0001] The present invention relates to a biological microparticle processing system, and particularly to a non-contact biological microparticle processing device and a biological microparticle processing apparatus.

Background Art

[0002] Conventional biological microparticle processing systems can perform related operations on biological microparticles in a liquid. However, since the biological microparticles flow due to the liquid, conventional biological microparticle processing systems perform related operations by a fixed-point method that fixes the biological microparticles. However, this is clearly disadvantageous for culturing the biological microparticles. Furthermore, whether the biological microparticles flow due to the liquid or are fixed, they are affected by the pressure of the liquid or are easily damaged.

Summary of the Invention

Problems to be Solved by the Invention

[0003] Therefore, the inventor of the present application believes that the above-mentioned defects can be improved, devotes to research, applies scientific principles, and finally proposes the present invention that is reasonably designed and effectively improves the above-mentioned defects.

Means for Solving the Problems

[0004] Embodiments of the present invention provide a non-contact biological microparticle processing device and a biological microparticle processing apparatus that can effectively improve defects that may occur in conventional biological microparticle processing systems.

[0005] Embodiments of the present invention disclose a non-contact type biological microparticle processing device. The non-contact type biological microparticle processing device comprises a biological microparticle processing device that accepts a first liquid and a second liquid insoluble in the first liquid, and a light driving device facing the biological microparticle processing device, wherein the biological microparticle processing device includes a droplet generation chamber that contains the first liquid and at least one biological microparticle located in the first liquid, a work chamber communicating with the droplet generation chamber, and a sorting chamber communicating with the work chamber, wherein the droplet generation chamber is used so that at least one of the biological microparticles and a portion of the first liquid surrounding it together form a biological microparticle droplet, and the work chamber contains the second liquid and the biological microparticle droplet so that the biological microparticles are contained within the second liquid of the work chamber. The particle droplets are flowed, and the first liquid in the biological microparticle droplets is used to perform a culture or detection operation on at least one of the biological microparticles. The sorting chamber contains the first liquid, thereby forming an immiscible interface between the work chamber and the sorting chamber. The optical drive device moves the biological microparticle droplets by causing a dielectric pattern to form in the biological microparticle processing apparatus. The optical drive device moves the biological microparticle droplets from the work chamber to the sorting chamber by the dielectric pattern, thereby dissolving the first liquid in the biological microparticle droplets into the first liquid in the sorting chamber and releasing at least one of the biological microparticles into the first liquid in the sorting chamber.

[0006] Embodiments of the present invention further disclose a non-contact type biological microparticle processing device. The non-contact type biological microparticle processing device comprises a biological microparticle processing device that receives a first liquid and a second liquid insoluble in the first liquid, and a light driving device facing the biological microparticle processing device, wherein the biological microparticle processing device includes a droplet generation chamber that contains the first liquid and at least one biological microparticle located in the first liquid, a work chamber that communicates with the droplet generation chamber, and a sorting chamber that communicates with the work chamber and has a discharge structure formed at its edge adjacent to the work chamber, wherein the droplet generation chamber is used for at least one of the biological microparticles and a portion of the first liquid surrounding it to together form a biological microparticle droplet, and the work chamber contains the second liquid and the biological microparticle droplet. By doing so, the biological microparticle droplets are flowed into the second liquid of the work chamber, and the first liquid of the biological microparticle droplets is used to perform a culture or detection operation on at least one of the biological microparticles. The sorting chamber contains the second liquid, and the optical drive device moves the biological microparticle droplets by causing the biological microparticle processing device to form a dielectric pattern. The optical drive device moves the biological microparticle droplets from the work chamber along the release structure to the sorting chamber by the dielectric pattern, destroys the biological microparticle droplets with the release structure, disperses the first liquid of the biological microparticle droplets, and releases at least one of the biological microparticles into the second liquid of the sorting chamber.

[0007] Embodiments of the present invention further disclose a biological microparticle processing apparatus. The biological microparticle processing apparatus accepts a first liquid and a second liquid insoluble in the first liquid. The biological microparticle processing apparatus includes a droplet generation chamber containing the first liquid, at least one biological microparticle located in the first liquid, and the second liquid; a work chamber communicating with the droplet generation chamber; and a sorting chamber communicating with the work chamber, wherein the droplet generation chamber crosses the flow of the second liquid with the first liquid so that at least one of the biological microparticles and a portion of the first liquid surrounding it pass through the second liquid and together form a biological microparticle droplet; the work chamber contains the second liquid so that the biological microparticle droplet flows into the second liquid in the work chamber, and the first liquid in the biological microparticle droplet performs a culture operation or detection operation on at least one of the biological microparticles; the sorting chamber contains the first liquid so that an immiscibility interface is formed between the work chamber and the sorting chamber, or the sorting chamber contains the second liquid so that a release structure is formed at the edge adjacent to the work chamber. [Effects of the Invention]

[0008] From the above, the non-contact type biological microparticle processing device and biological microparticle processing device disclosed in the embodiments of the present invention can obtain a protective effect by coating at least one of the biological microparticles in the first liquid by forming biological microparticle droplets suspended in the second liquid. Consequently, the biological microparticle droplets can move rapidly through the second liquid without damaging at least one of the biological microparticles located within the droplets. Furthermore, while moving the biological microparticle droplets, the culture operation or detection operation of at least one of the biological microparticles located within the droplets can also be completed.

[0009] To further understand the features and technical content of the present invention, please refer to the following detailed description and drawings of the present invention, however, these descriptions and drawings are intended solely to illustrate the present invention and do not limit the scope of protection of the present invention in any way. [Brief explanation of the drawing]

[0010] [Figure 1] This is a schematic three-dimensional diagram of a non-contact biological microparticle processing device according to Embodiment 1 of the present invention. [Figure 2] Figure 1 is a schematic longitudinal cross-sectional view of a non-contact biological microparticle processing device. [Figure 3] This is a schematic cross-sectional view of a non-contact biological microparticle processing device according to Embodiment 1 of the present invention. [Figure 4] Figure 3 is a schematic cross-sectional view of multiple biological microparticle droplets formed in a non-contact biological microparticle processing device. [Figure 5] This is a schematic longitudinal cross-sectional view of a non-contact biological microparticle processing device according to Embodiment 1 of the present invention. [Figure 6] Figure 3 is a schematic diagram illustrating the subsequent operation. [Figure 7] Figure 6 is a schematic diagram illustrating the subsequent operation. [Figure 8] This is a schematic cross-sectional view showing another embodiment of the non-contact biological microparticle processing device according to Embodiment 1 of the present invention. [Figure 9] This is a schematic cross-sectional view of a non-contact biological microparticle processing device according to Embodiment 2 of the present invention. [Figure 10] Figure 9 is a schematic longitudinal cross-sectional view of a non-contact biological microparticle processing device. [Figure 11] Figure 9 is a schematic diagram illustrating the subsequent operation. [Figure 12] Figure 11 is a schematic longitudinal cross-sectional view of a non-contact biological microparticle processing device. [Figure 13] Figure 11 is a schematic diagram illustrating the subsequent operation. [Figure 14] Figure 13 is a schematic longitudinal cross-sectional view of a non-contact type biological microparticle processing device. [Figure 15] This is a schematic cross-sectional view showing another embodiment of a non-contact biological microparticle processing device according to Example 2 of the present invention. [Figure 16] It is a schematic longitudinal sectional view of the non-contact biological microparticle processing device of FIG. 15. [Figure 17] It is a schematic subsequent operation diagram of FIG. 15. [Figure 18] It is a schematic longitudinal sectional view of the non-contact biological microparticle processing device of FIG. 17.

Embodiments for Carrying Out the Invention

[0011] Hereinafter, embodiments of the "non-contact biological microparticle processing device and biological microparticle processing apparatus" disclosed in the present invention will be described by way of specific examples. A person skilled in the art can understand the advantages and effects of the present invention from the disclosed content. The present invention can be implemented or applied through other different specific embodiments, and various detailed descriptions in this specification can also be modified and changed in various ways without departing from the idea of the present invention based on different viewpoints and uses. Also, it should be explained in advance that the drawings of the present invention are merely schematic and are not drawn based on actual dimensions. The technical content of the present invention will be described in more detail using the following embodiments, but the disclosed content is not intended to limit the protection scope of the present invention.

[0012] In this specification, terms such as "first", "second", etc. may be used to describe various elements, but it should be understood that these elements should not be limited by these terms. These terms are mainly used to distinguish one element from another or one feature from another. Also, the term "or" in this specification should be understood to include any one or a combination of multiple of the related listed items according to the actual situation.

[0013] [Example 1] Embodiment 1 of the present invention will be described with reference to FIGS. 1 to 8. As shown in FIGS. 1 to 3, this embodiment discloses a non-contact biological microparticle processing device 100 that performs a culturing operation or a detection operation on at least one biological microparticle B. Here, the biological microparticle B may be a specific type of cell or cell cluster, for example, circulating tumor cells (CTC), fetal nucleated red blood cells (FNRBCs), virus, microorganism, or bacterium. However, the present invention is not limited thereto.

[0014] The non-contact biological microparticle processing device 100 includes a biological microparticle processing apparatus 1, an alternating current device 2 electrically coupled to the biological microparticle processing apparatus 1, and an optical driving device 3 facing the biological microparticle processing apparatus 1. However, the present invention is not limited thereto. For example, in other embodiments not shown in this specification, the biological microparticle processing apparatus 1 may be used (for example, sold) alone according to actual needs, or may be used in combination with other devices.

[0015] In this embodiment, the biological microparticle processing apparatus 1 is a chip-scale rectangular structure. Further, the biological microparticle processing apparatus 1 receives (or includes) a first liquid L1, at least one of the biological microparticles B located in the first liquid L1, and a second liquid L2 insoluble in the first liquid L1. For example, the first liquid L1 includes oil and a surfactant, and the second liquid is water. Or, the first liquid L1 includes water and a surfactant, and the second liquid is oil. However, the present invention is not limited thereto.

[0016] Furthermore, the optical driving device 3 may form a dielectrophoresis (DEP) pattern F (for example, FIG. 6) on the biological microparticle processing apparatus 1. The structure of the biological microparticle processing apparatus 1 capable of realizing the dielectrophoresis pattern F is generally as follows. However, the present invention is not limited thereto.

[0017] In this embodiment, the biological microparticle processing apparatus 1 includes a light-sensing module 11, a matching module 12 provided at a distance from the light-sensing module 11, and a bonding layer 13 that joins the periphery of the light-sensing module 11 and the periphery of the matching module 12. Here, at least one of the light-sensing module 11 and the matching module 12 is transparent, and in this embodiment, the light-sensing module 11 and the matching module 12 are two plate-like structures arranged parallel to each other, and the distance between them is greater than the size of either of the biological microparticles B. However, the present invention is not limited to these.

[0018] More specifically, the light sensing module 11 includes a first substrate 111, a first electrode layer 112 formed on the first substrate 111, and a photoelectric layer 113 formed on the first substrate 111. In this embodiment, the first electrode layer 112 is formed on the bottom side of the first substrate 111, and the photoelectric layer 113 is formed on the top side of the first substrate 111. Here, a plurality of transistors arranged in a matrix are formed on the photoelectric layer 113. Furthermore, the photoelectric layer 113 can employ an NPN transistor structure, a PNP transistor structure, an NP diode structure, or a PN diode structure depending on the actual requirements. However, the present invention is not limited thereto.

[0019] The matching module 12 includes a second substrate 121 and a second electrode layer 122 formed on the second substrate 121. Furthermore, the second electrode layer 122 faces the light sensing module 11 (for example, the photoelectric layer 113). Here, the AC device 2 is electrically coupled to the first electrode layer 112 of the light sensing module 11 and the second electrode layer 122 of the matching module 12.

[0020] Accordingly, as shown in Figures 2 and 5 to 7, the optical drive device 3 causes the photodetector module 11 to form the dielectric pattern F by emitting light and irradiating it. In this embodiment, the optical drive device 3 may include a camera 31 and a light source 32 corresponding to the camera 31. Here, the optical drive device 3 causes the photodetector module 11 (or the photoelectric layer 113) to form the dielectric pattern F by irradiating the photodetector module 11 with light emitted from the light source 32.

[0021] From another perspective, the internal structure of the biological microparticle processing apparatus 1 includes a droplet generation chamber 14, a work chamber 15 communicating with the droplet generation chamber 14, and a sorting chamber 16 communicating with the work chamber 15. In this embodiment, the droplet generation chamber 14, the work chamber 15, and the sorting chamber 16 are arranged between the light sensing module 11 and the matching module 12. Furthermore, in this embodiment, the droplet generation chamber 14 and the sorting chamber 16 are each connected to opposing sides of the work chamber 15. However, the present invention is not limited thereto.

[0022] The droplet generation chamber 14 contains the first liquid L1 and at least one of the biological microparticles B located within the first liquid L1. Here, the droplet generation chamber 14 is used so that at least one of the biological microparticles B and a portion of the first liquid L1 surrounding it can together form a biological microparticle droplet P.

[0023] It should be noted that, in order to facilitate understanding of this embodiment, the following description first explains that one biological microparticle droplet P is formed in the biological microparticle processing apparatus 1, but the present invention is not limited thereto. For example, as shown in Figure 4, multiple biological microparticle droplets P may exist simultaneously in the biological microparticle processing apparatus 1, and furthermore, the number of biological microparticles B in any one of the biological microparticle droplets P may be more than one as required in practice.

[0024] Furthermore, assuming that the biomicroparticle droplets P can be formed, the droplet generation chamber 14 may be designed according to actual needs. For example, in other embodiments not shown herein, the droplet generation chamber 14 disperses the first liquid L1 into a plurality of droplets by physical means (e.g., stirring or vibration), and defines the biomicroparticle droplet P as a droplet coated with at least one of the biomicroparticles B.

[0025] Furthermore, in this embodiment, the droplet generation chamber 14 forms the biological microparticle droplets P by a fluid method in order to reduce damage to the biological microparticles B. Here, the droplet generation chamber 14 further contains the second liquid L2, and by crossing the flow of the second liquid L2 with the first liquid L1, at least one of the biological microparticles B and the portion of the first liquid L1 surrounding it pass through the second liquid L2 and together form the biological microparticle droplets P.

[0026] More specifically, the droplet generation chamber 14 includes a first channel 141 and a second channel 142 that intersects the first channel 141 (perpendicularly), and the first channel 141 and the second channel 142 intersect each other to form a confluence region 143 that communicates with the work chamber 15. Here, the first channel 141 introduces the first liquid L1 and at least one of the biological microparticles B, and the second channel 142 introduces the second liquid L2. Furthermore, at least one of the biological microparticles B and the portion of the first liquid L1 surrounding it together form the biological microparticle droplet P after passing through the confluence region 143.

[0027] The work chamber 15 can contain the second liquid L2 and the biological microparticle droplets P, allowing the biological microparticle droplets P to flow into the second liquid L2 of the work chamber 15, and the dielectric pattern F can move (for example, press) the biological microparticle droplets P into the work chamber 15. Furthermore, the biological microparticle droplets P located in the work chamber 15 can be cultured or detected by the first liquid L1 for at least one of the biological microparticles B located within the biological microparticle droplets P.

[0028] In this embodiment, the first liquid L1 of the biological microparticle droplet P contains at least one of a medium, a peptide, and a recombinant protein so as to perform the culture operation on at least one of the biological microparticles B. Alternatively, the first liquid L1 of the biological microparticle droplet P contains at least one of a detection reagent and chemicals so as to perform the detection operation on at least one of the biological microparticles B.

[0029] As described above, in this embodiment, the biological microparticle processing apparatus 1 can obtain a protective effect by coating at least one of the biological microparticles B within the first liquid L1 by forming the biological microparticle droplet P suspended in the second liquid L2. Consequently, the biological microparticle droplet P can move rapidly within the second liquid L2 without damaging at least one of the biological microparticles B. Furthermore, the culture operation or detection operation of at least one of the biological microparticles B can be completed while moving the biological microparticle droplet P.

[0030] It should be noted that a waste port 151 is formed in the work chamber 15, and the optical drive device 3 can selectively move the biological microparticle droplets P from the work chamber 15 to the sorting chamber 16 or the waste port 151 using the dielectrophoresis pattern F. That is, if the culture operation or detection operation is performed on the biological microparticle droplets P and the result is unsuccessful, the biological microparticle droplets P are moved to the waste port 151 by the dielectrophoresis pattern F and removed from the biological microparticle processing device 1. If the result is successful, the biological microparticle droplets P are moved into the sorting chamber 16 by the dielectrophoresis pattern F.

[0031] Furthermore, the sorting chamber 16 contains the first liquid L1, thereby forming an immiscible interface L3 between the work chamber 15 and the sorting chamber 16. Accordingly, the optical drive device 3 can move the biological microparticle droplets P from the work chamber 15 to the sorting chamber 16 by the dielectric pattern F. In this way, the first liquid L1 of the biological microparticle droplets P is dissolved in the first liquid L1 of the sorting chamber 16, and at least one of the biological microparticles B is released into the first liquid L1 of the sorting chamber 16.

[0032] Furthermore, a collection port 161 may be formed in the sorting chamber 16, and at least one of the biological microparticles B located within the sorting chamber 16 is discharged from the biological microparticle processing apparatus 1 through the collection port 161. Here, the movement of at least one of the biological microparticles B within the sorting chamber 16 may be achieved by the dielectric pattern F (for example, Figures 6 and 7) or by hydraulic pressure control (for example, the hydraulic pressure control port 163 provided in Figure 8).

[0033] [Example 2] Embodiment 2 of the present invention will be described with reference to Figures 9 to 18. Since this embodiment is similar to Embodiment 1 described above, a detailed explanation of the similarities between the two embodiments will be omitted. The main difference between this embodiment and Embodiment 1 described above is the sorting chamber 16.

[0034] In this embodiment, the sorting chamber 16 contains the second liquid L2, and the photosensing module 11 includes an insulating layer 114 formed on the photoelectric layer 113. Furthermore, the sorting chamber 16 has a release structure 162 formed at its edge adjacent to the work chamber 15 in order to break the surface tension of the biological particulate droplets P. In this embodiment, the release structure 162 includes a plurality of protrusions 1621 arranged along the edge of the work chamber 15, and it is preferable that the distance between any two adjacent protrusions 1621 is smaller than the outer diameter of the biological particulate droplets P. However, the present invention is not limited thereto.

[0035] It should be noted that, as shown in Figures 9 to 14, if the density of the first liquid L1 used in the biological microparticle processing apparatus 1 is greater than the density of the second liquid L2, the biological microparticle droplets P tend to sink in the second liquid L2, so the release structure 162 is formed on the insulating layer 114. Also, as shown in Figures 15 to 18, if the density of the first liquid L1 used in the biological microparticle processing apparatus 1 is less than the density of the second liquid L2, the biological microparticle droplets P tend to float in the second liquid L2, so the release structure 162 is formed on the matching module 12.

[0036] As described above, the optical drive device 3 can move the biological microparticle droplets P from the work chamber 15 along the release structure 162 to the sorting chamber 16 by means of the dielectric pattern F. In this way, the biological microparticle droplets P are destroyed by the release structure 162, the first liquid L1 of the biological microparticle droplets P is dispersed, and at least one of the biological microparticles B is released into the second liquid L2 of the sorting chamber 16.

[0037] [Beneficial effects of the embodiment] From the above, the non-contact type biological microparticle processing device and biological microparticle processing device disclosed in the embodiments of the present invention can obtain a protective effect by forming biological microparticle droplets suspended in the second liquid, thereby coating the first liquid with at least one of the biological microparticles. Consequently, the biological microparticle droplets can move rapidly through the second liquid without damaging at least one of the biological microparticles located within the droplets. Furthermore, while moving the biological microparticle droplets, the culture operation or detection operation of at least one of the biological microparticles located within the droplets can also be completed.

[0038] The information disclosed above represents only preferred embodiments of the present invention and does not limit the scope of the claims. Accordingly, all equivalent technical modifications made using the specification and drawings of the present invention are included within the scope of the claims. [Explanation of Symbols]

[0039] 100: Non-contact type biological microparticle processing equipment 1: Biomicroparticle processing device 11: Light sensing module 111: First board 112: First electrode layer 113: Photoelectric layer 114: Insulating layer 12: Alignment Module 121: Second board 122: Second electrode layer 13: Laminate layer 14: Droplet generation chamber 141: First channel 142: Second channel 143: Merging area 15: Working Chamber 151: Disposal port 16: Sorting Chamber 161: Collection point 162:Emission structure 1621: Protrusion 163: Hydraulic control port 2: AC device 3: Optical drive device 31: Camera 32: Light source L1: 1st liquid L2: 2nd liquid L3: Immiscible interface B: Biological microparticles P: Biomicroparticle droplets F: Dielectrophoresis pattern

Claims

1. A biological microparticle processing apparatus that accepts a first liquid and a second liquid insoluble in the first liquid, A light driving device facing the aforementioned biological microparticle processing device, Equipped with, The aforementioned biological microparticle processing device is A droplet generation chamber containing the first liquid and at least one biological microparticle located within the first liquid, A working chamber connected to the aforementioned droplet generation chamber, A sorting chamber connected to the aforementioned work chamber, Includes, The droplet generation chamber is used so that at least one of the biological microparticles and a portion of the first liquid surrounding it can together form a biological microparticle droplet. The work chamber contains the second liquid and the biological microparticle droplets, allowing the biological microparticle droplets to flow into the second liquid of the work chamber, and performing a culture or detection operation on at least one of the biological microparticles using the first liquid of the biological microparticle droplets. The sorting chamber, by containing the first liquid, forms an immiscible interface between the work chamber and the sorting chamber. The optical drive device moves the biological microparticle droplets by causing the biological microparticle processing device to form a dielectric pattern. The optical drive device moves the biological microparticle droplets from the work chamber to the sorting chamber by the dielectric pattern, thereby dissolving the first liquid of the biological microparticle droplets in the first liquid of the sorting chamber, and releasing at least one of the biological microparticles into the first liquid of the sorting chamber. The aforementioned biological microparticle processing device is A light sensing module having a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate, Matching modules are provided at intervals on the aforementioned light sensing module, Includes, At least one of the light sensing module and the matching module is transparent, and the matching module includes a second substrate and a second electrode layer formed on the second substrate, the second electrode layer facing the light sensing module, The light driving device emits light and irradiates the light sensing module to form the dielectric pattern on the light sensing module. A non-contact type biological microparticle processing device characterized by the following features.

2. The droplet generation chamber contains the second liquid and, by crossing the flow of the second liquid with the first liquid, at least one of the biological microparticles and the portion of the first liquid surrounding it pass through the second liquid and together form the biological microparticle droplet. The non-contact type biological microparticle processing device according to claim 1.

3. The aforementioned droplet generation chamber is A first channel through which the first liquid and at least one of the biological microparticles are introduced, A second channel intersects with the first channel and forms a confluence region that communicates with the work chamber, Includes, The second channel introduces the second liquid, and at least one of the biological microparticles and the portion of the first liquid surrounding it pass through the confluence region and together form the biological microparticle droplet. The non-contact type biological microparticle processing device according to claim 2.

4. A waste port is formed in the work chamber, and the optical drive device selectively moves the biological microparticle droplets from the work chamber to the sorting chamber or the waste port according to the dielectric pattern. The non-contact type biological microparticle processing device according to claim 1.

5. The first liquid of the biological microparticle droplet contains at least one of a culture medium, a peptide, and a recombinant protein, so as to perform the culture operation on at least one of the biological microparticles, or the first liquid of the biological microparticle droplet contains at least one of a detection reagent and chemicals so as to perform the detection operation on at least one of the biological microparticles. The non-contact type biological microparticle processing device according to claim 1.

6. A biological microparticle processing apparatus that accepts a first liquid and a second liquid insoluble in the first liquid, A light driving device facing the aforementioned biological microparticle processing device, Equipped with, The aforementioned biological microparticle processing device is A droplet generation chamber containing the first liquid and at least one biological microparticle located within the first liquid, A working chamber connected to the aforementioned droplet generation chamber, A sorting chamber that is in communication with the work chamber and has a discharge structure formed at its edge adjacent to the work chamber, Includes, The droplet generation chamber is used so that at least one of the biological microparticles and a portion of the first liquid surrounding it can together form a biological microparticle droplet. The work chamber contains the second liquid and the biological microparticle droplets, allowing the biological microparticle droplets to flow into the second liquid of the work chamber, and performing a culture or detection operation on at least one of the biological microparticles using the first liquid of the biological microparticle droplets. The sorting chamber contains the second liquid, The optical drive device moves the biological microparticle droplets by causing the biological microparticle processing device to form a dielectric pattern. The optical drive device moves the biological microparticle droplets from the work chamber along the release structure to the sorting chamber by the dielectric pattern, destroys the biological microparticle droplets with the release structure, disperses the first liquid of the biological microparticle droplets, and releases at least one of the biological microparticles into the second liquid of the sorting chamber. The aforementioned biological microparticle processing device is A light sensing module having a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer, Matching modules are provided at intervals on the aforementioned light sensing module, Includes, At least one of the light sensing module and the matching module is transparent, and the matching module includes a second substrate and a second electrode layer formed on the second substrate. The light driving device emits light and irradiates the light sensing module to form the dielectric pattern on the light sensing module. A non-contact type biological microparticle processing device characterized by the following features.

7. The density of the first liquid is greater than the density of the second liquid, and the discharge structure is formed in the insulating layer, or the density of the first liquid is less than the density of the second liquid, and the discharge structure is formed in the matching module. The non-contact type biological microparticle processing device according to claim 6.

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