Double-confinement-region nanopore protein compound and application thereof

By assembling HfaB and truncated HfaA proteins in vitro to form a dual-restriction region nanoporous protein complex, the problem of low accuracy in nanopore sequencing was solved, and the ability to distinguish homopolymers and the stability of sequencing current were improved.

CN120965840APending Publication Date: 2025-11-18BEIJING POLYSEQ BIOTECH CO LTD +1
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Patent Information

Application Number
CN202410612475.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-05-16
Publication Date
2025-11-18

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Abstract

The invention discloses a nanopore protein compound with double confinement regions and application of the nanopore protein compound. The nanopore protein complex comprises an HfaB protein and a truncated HfaA protein, and the truncated HfaA protein is connected with the HfaB protein and forms a confinement region in the nanopore protein complex. The novel double-confinement-region nanopore protein complex is provided in an in-vitro assembly mode, the types of nanopore proteins are enriched, and compared with a single-confinement-region nanopore protein oligomer state and sequencing current before assembly, the double-confinement-region nanopore protein complex is more stable.
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