An anti-wrinkle firming composition and its use, cosmetic

Abstract

The application belongs to the technical field of cosmetic production and discloses an anti-wrinkle firming composition and purposes and cosmetics thereof, which comprise oregano leaf extract and tribulus terrestris fruit extract, and the mass ratio of the oregano leaf extract to the tribulus terrestris fruit extract is 1-10:5-15. The application can effectively provide good anti-wrinkle firming effect for the skin in the aspects of free radical scavenging and elastase inhibition capacity improvement through the synergistic effect between the oregano leaf extract and the tribulus terrestris fruit extract. In addition, the anti-wrinkle firming capacity of the composition can be further improved after adding yarrow extract to the composition.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetic production, in particular to an anti-wrinkle firming composition, application and cosmetic thereof. BACKGROUND

[0002] Extracellular matrix (ECM) is an important component of skin structure, mainly composed of collagen, elastin, hyaluronic acid, glycosaminoglycan, etc., which is crucial for maintaining skin elasticity, firmness and moisture. Collagen is the main component of ECM, providing mechanical strength and support for the skin. With age, collagen synthesis decreases and degradation increases, leading to skin laxity and wrinkle formation; elastin gives the skin elasticity, allowing it to return to its original shape after stretching. Aging or photodamage can damage elastin, causing the skin to lose elasticity and form wrinkles; hyaluronic acid can absorb a large amount of water, keeping the skin moist and plump. With age, hyaluronic acid decreases, causing the skin to dry out and collapse, forming fine lines; glycosaminoglycans maintain skin moisture and structural integrity by binding to water molecules. Their reduction can cause skin dryness and structural damage, leading to wrinkles, so maintaining ECM at a high level is crucial for skin anti-wrinkle.

[0003] Prior Art 1: Chinese Patent Application 202410745253.5 discloses an anti-wrinkle repair composition and its application, which includes polypeptides, plant extracts and collagen; the polypeptides include acetyl hexapeptide-8, snake venom-like peptide and arginine / lysine polypeptide; the plant extracts include Sophora flavescens root extract, Tribulus terrestris fruit extract and Cornus officinalis fruit extract;

[0004] Further observation of the description of this scheme in paragraph 9 shows that the scheme points out that "it is found that the plant extract combination of Sophora flavescens root extract, Tribulus terrestris fruit extract and Cornus officinalis fruit extract also has a potential synergistic relationship, which can further improve the anti-wrinkle repair effect of the product and achieve an instant soothing effect."

[0005] However, this scheme does not attempt to determine whether Tribulus terrestris and other plant ingredients (such as oregano in the present case) have a synergistic relationship to improve the anti-wrinkle repair effect;

[0006] Prior art 2: KR100971655B1 discloses a cosmetic composition with antioxidant, anti-inflammatory, and anti-wrinkle effects, relating to a mixture prepared by low-temperature ultrasonic extraction of herbs, with lavender, rosemary, oregano, and thyme as main components. This solution also provides a cosmetic composition that exhibits excellent antioxidant, anti-inflammatory, and anti-wrinkle effects due to the presence of herbal extracts.

[0007] Further observation of the scheme reveals that it first mixes the above substances and ferments them with Bacillus, then extracts them to obtain the final extract.

[0008] It is evident that existing technologies have some records of the anti-wrinkle effects of oregano, but fermentation is needed to increase the content of anti-wrinkle active ingredients in the extract;

[0009] In summary, current technology suggests that Tribulus terrestris and oregano have certain anti-wrinkle or skin-lifting effects, but current technology has not explored the effects of using the two in combination.

[0010] The problem this solution aims to solve is: how to provide an anti-wrinkle composition with good firming and antioxidant capabilities. Summary of the Invention

[0011] The purpose of this application is to provide an anti-wrinkle and firming composition in which oregano leaf extract can promote collagen production and, to a certain extent, reduce the content of matrix metalloproteinases (MMPs) and free radicals through its antioxidant mechanism. In addition, tribulus fruit extract in the composition is rich in saponins and flavonoid active ingredients, which can stimulate the production of collagen and elastin. The synergistic effect of the two can effectively increase the content of extracellular matrix in skin cells by improving collagen, improving elastin, and inhibiting matrix metalloproteinases, thereby achieving a good anti-wrinkle effect.

[0012] To achieve the above objectives, this application discloses an anti-wrinkle and firming composition comprising oregano leaf extract and tribulus fruit extract, wherein the mass ratio of oregano leaf extract to tribulus fruit extract is 1-10:5-15.

[0013] It should be further noted that oregano leaf extract prevents skin sagging by scavenging free radicals and reducing the degradation of collagen and elastin.

[0014] Tribulus terrestris fruit extract can protect the integrity of protein structures in the skin and enhance the skin's barrier function, thereby inhibiting the formation of wrinkles.

[0015] Preferably, it also includes yarrow extract, wherein the mass ratio of oregano leaf extract, tribulus fruit extract and yarrow extract is 1-10:5-15:1-5.

[0016] Preferably, the extraction method of the yarrow extract is as follows: drying and crushing the yarrow, then mixing it with pure water, heating, stirring, centrifuging, and concentrating to obtain an intermediate;

[0017] Then, an equal volume of ethanol was added to the intermediate, the precipitate was removed after alcohol precipitation, the ethanol was evaporated to dryness and butylene glycol was added, followed by ultrafiltration and sterilization to obtain yarrow extract.

[0018] Preferably, the extraction method of the Tribulus terrestris fruit extract is as follows: drying and crushing the Tribulus terrestris fruit, then mixing it with pure water, heating, stirring, centrifuging, and concentrating to obtain an intermediate;

[0019] Then, an equal volume of ethanol was added to the intermediate, the precipitate was removed after alcohol precipitation, the ethanol was evaporated to dryness, butanediol was added, ultrafiltration was performed, and sterilization was carried out to obtain Tribulus terrestris fruit extract.

[0020] Preferably, the extraction method of the oregano leaf extract is as follows: drying and crushing oregano leaves, then mixing with pure water, heating, stirring, centrifuging, and concentrating to obtain an intermediate;

[0021] Then, an equal volume of ethanol was added to the intermediate, the precipitate was removed after alcohol precipitation, the ethanol was evaporated to dryness and butylene glycol was added, followed by ultrafiltration and sterilization to obtain oregano leaf extract.

[0022] In addition, this application also discloses the use of the anti-wrinkle and firming composition as described above in the preparation of cosmetics.

[0023] In addition, this application also discloses a cosmetic containing 0.1 to 30 wt% of the anti-wrinkle and firming composition as described above.

[0024] Preferably, the dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion, or cream.

[0025] Preferably, the cosmetic is a gel, serum, toner, face mask, facial cleanser, lotion, perfume, makeup remover, lipstick, blush, or spray.

[0026] The beneficial effects of this application are:

[0027] In the composition of this application, oregano leaf extract prevents skin sagging by scavenging free radicals and reducing the degradation of collagen and elastin. Tribulus terrestris fruit extract protects the integrity of protein structures in the skin and enhances the skin's barrier function, thereby inhibiting wrinkle formation. In summary, the two complement each other and synergistically enhance the anti-wrinkle and firming effect. Attached Figure Description

[0028] Fig. 1 The image shows the chicken embryo of the sample corresponding to Example 5 before the irritation test;

[0029] Fig. 2The image shows the chicken embryo of the sample corresponding to Example 5 after the irritation test. Detailed Implementation

[0030] The present application will be clearly and completely described below with reference to its embodiments. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0031] Before demonstrating the embodiments, the preparation and acquisition methods of the raw materials involved in the embodiments shall be explained as follows:

[0032] Yarrow: Taobao store (store name "Xiang Shou Yi Sheng");

[0033] Tribulus terrestris fruit: Trade name "White Tribulus terrestris", Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0034] Oregano leaves: Taobao store (store name "Qishantang Flagship Store");

[0035] Ethanol: Guangxi Jinyuan Biochemical Industry Co., Ltd., purity over 95%;

[0036] Butylene glycol: Guangzhou Hemiao Biotechnology Co., Ltd.;

[0037] 1,2-Hexanediol: Guangzhou Hemiao Biotechnology Co., Ltd.;

[0038] Extraction method of yarrow extract: Dry and crush yarrow, then mix yarrow with pure water at a volume ratio of 1:10 and stir at 65±5℃ for 3±0.5h, centrifuge, filter, and collect the filtrate.

[0039] The mass of the system was then concentrated to 30±5% of the initial mass to obtain an intermediate (specifically, in this case, the total mass of the system was concentrated from 1 kg to 300±5 g).

[0040] Then, an equal volume of ethanol was added to the intermediate, and the mixture was precipitated. The precipitate was then removed, the ethanol was evaporated to dryness, and 30±3% butanediol was added to the mixture. The mixture was then ultrafiltered (0.1-0.3 MPa), and the filtrate was collected and sterilized to obtain the yarrow extract.

[0041] Extraction method of Tribulus terrestris fruit extract: Dry and crush Tribulus terrestris fruit, then mix Tribulus terrestris fruit with water at a volume ratio of 1:10 and stir at 65±5℃ for 3±0.5h, then centrifuge, filter and collect the filtrate.

[0042] The mass of the system was then concentrated to 30±5% of the initial mass to obtain an intermediate (specifically in this case, the total mass of the system was concentrated from 1 kg to 300±5 g).

[0043] Then, an equal volume of ethanol was added to the intermediate, and the mixture was precipitated. The precipitate was then removed, the ethanol was evaporated to dryness, and 30±3% butanediol was added to the mixture. The mixture was then ultrafiltered (0.1-0.3 MPa), and the filtrate was collected and sterilized to obtain Tribulus terrestris fruit extract.

[0044] Extraction method of oregano leaf extract: Dry and crush oregano leaves, then mix oregano leaves with water at a volume ratio of 1:10 and stir at 65±5℃ for 3±0.5h, centrifuge, filter, and collect the filtrate.

[0045] The mass of the system was then concentrated to 30±5% of the initial mass to obtain an intermediate (specifically in this case, the total mass of the system was concentrated from 1 kg to 300±5 g).

[0046] Then, an equal volume of ethanol was added to the intermediate, and the mixture was precipitated. The precipitate was then removed, the ethanol was evaporated to dryness, and 30±3% butanediol was added to the mixture. The mixture was then ultrafiltered (0.1-0.3 MPa), and the filtrate was collected and sterilized to obtain oregano leaf extract.

[0047] Example 1

[0048] An anti-wrinkle and firming composition was obtained by mixing oregano leaf extract and tribulus fruit extract at a mass ratio of 1:15.

[0049] Example 2

[0050] The composition is basically the same as in Example 1, except that the mass ratio of oregano leaf extract to tribulus fruit extract is adjusted to 10:5 while keeping the total mass of the composition the same.

[0051] Example 3

[0052] The composition is basically the same as in Example 1, except that the mass ratio of oregano leaf extract to tribulus fruit extract is adjusted to 6:12 while keeping the total mass of the composition the same.

[0053] Example 4

[0054] Oregano leaf extract, tribulus fruit extract and yarrow extract were mixed in a mass ratio of 6:12:1 to obtain an anti-wrinkle and firming composition with the same total mass as the composition in Example 1.

[0055] Example 5

[0056] The results are basically the same as in Example 4, except that the mass ratio of oregano leaf extract, tribulus fruit extract and yarrow extract is adjusted to 6:12:2, while keeping the total mass of the composition the same.

[0057] Example 6

[0058] The results are basically the same as in Example 4, except that the mass ratio of oregano leaf extract, tribulus fruit extract and yarrow extract is adjusted to 6:12:5, while keeping the total mass of the composition the same.

[0059] Comparative Examples 1-5

[0060] A composition, the total mass of which is the same as that in Example 1, differs in the mass ratio of each extract, as shown in Table 1:

[0061] Table 1: Formulation Table for Comparative Examples 1-5

[0062]

[0063]

[0064] Performance testing:

[0065] 1. Stimulation test

[0066] 1.1 Instruments and Equipment

[0067] Fully automatic incubator, stereo microscope, SPF chicken embryos;

[0068] 1.2 Reagents

[0069] Sodium chloride, sodium dodecyl sulfate (SDS);

[0070] 1.3 Incubation conditions

[0071] Room temperature 20℃~25℃, relative humidity 45%~70%, incubation temperature 37.5℃±0.5℃, relative humidity 55%~70%, turntable 3 to 6 times / h, no rotation is required when incubating 9-day-old chicken embryos.

[0072] 1.4 Test Methods

[0073] (1) Experimental operation procedures

[0074] In this test, six embryos were selected for each group. The condition of the chorioallantoic membrane was recorded using a photographic device. A polytetrafluoroethylene (PTFE) resin ring was placed on the chorioallantoic membrane of the chicken embryo, and the image was taken and recorded. The composition prepared in Example 5 was diluted with pure water to a 2% (w / w) solution and used as the test sample. The test sample was then added to the PTFE resin ring, and the time of sample addition was recorded. The air cell was covered with a moistened plastic wrap, and the chicken embryos were transferred to a constant temperature and humidity incubator for culture. The degree of change in toxicity effects was observed.

[0075] (2) Results observation

[0076] Observe and record bleeding, coagulation, and vascularization manifestations, and score them according to their severity.

[0077] The scoring criteria for bleeding, coagulation, and angiogenesis are shown in Table 2:

[0078] Table 2: Stimulation Test Scoring Sheet

[0079]

[0080]

[0081] Scoring criteria: ES≤4, non-irritating; 4<ES≤12, mildly irritating; 12<ES<16, moderately irritating; ES≥16, highly irritating / corrosive.

[0082] refer to Figs. 1-2 Upon testing, the ES value of the sample corresponding to Example 5 was 2.00, which is less than 4, indicating that it is non-irritating.

[0083] 2. Elastase inhibition rate test

[0084] 2.1 Instruments and Equipment

[0085] BSA224S analytical balance, RT-6100 enzyme-linked immunosorbent assay (ELISA) analyzer;

[0086] 2.2 Reagents

[0087] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0088] 2.3 Test Methods

[0089] (1) Treatment of control materials and test samples

[0090] Sample group: Dilute the samples (compositions prepared in the examples and comparative examples) with pure water to a mass fraction of 5%;

[0091] Negative control: pure water.

[0092] (2) Experimental Operation Procedures

[0093] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group should be set up in 3 replicates. Add different reagent solutions to 96-well plates, shake gently, incubate at 25°C for 15 min, and then place them in an ELISA reader to measure the absorbance at 410 nm.

[0094] (3) The calculation formula is shown in Equation 1:

[0095]

[0096] In the formula: A—is the absorbance of the reaction solution without sample and the reaction solution containing enzyme;

[0097] B—The absorbance of the reaction solution without the sample and without the enzyme;

[0098] C—is the absorbance of the sample and the reaction solution containing the enzyme;

[0099] D—The absorbance of the reaction solution containing the sample and the reaction solution without the enzyme.

[0100] The test results are shown in Table 3:

[0101] Table 3: Results of Elastase Inhibition Rate Test

[0102]

[0103]

[0104] Results analysis:

[0105] 1. As can be seen from Examples 1-3, when the composition is a mixture of oregano leaf extract and tribulus fruit extract, the elastase inhibition rate of the composition gradually increases as the proportion of oregano leaf extract in the composition increases;

[0106] Further observation of Example 4 shows that when yarrow extract is added to the composition, its elastase inhibition rate shows a further increasing trend compared to Example 3. At the same time, in Examples 5-6, when the mass ratio of oregano leaf extract, tribulus fruit extract and yarrow extract is 6:12:5, its elastase inhibition rate is more advantageous.

[0107] 2. As can be seen from Example 1 and Comparative Examples 1-2, when the composition contains only a single oregano leaf extract or tribulus fruit extract, the elastase inhibition rate of Comparative Example 1 is only 21.57%, and the elastase inhibition rate of Comparative Example 2 is only 18.62%, which is much lower than the 27.98% of Example 3.

[0108] It is evident that the oregano leaf extract and tribulus fruit extract produced a synergistic effect when used together, resulting in the elastase inhibitory capacity of the composition prepared in Example 3 exceeding that of either Comparative Examples 1-2.

[0109] Further observation of Comparative Examples 3-5 revealed that when the composition of Comparative Example 3 was a mixture of oregano leaf extract and yarrow extract, its elastase inhibition rate reached 22.39%. When Comparative Example 5 used yarrow extract alone, the elastase inhibition rate was 23.25%. Further analysis of Comparative Example 1 showed that when Comparative Example 1 used oregano leaf extract alone, its elastase inhibition rate was 21.57%. The elastase inhibition rate of Comparative Example 3 was between that of Comparative Example 1 and Comparative Example 5. Therefore, there may be no synergistic effect or a weak synergistic effect between oregano leaf extract and yarrow extract.

[0110] Similarly, comparing Comparative Examples 2, 4, and 5, it can be seen that the elastase inhibition rate of Comparative Example 4 is also between that of Comparative Example 2 and Comparative Example 5. This suggests that there may be no synergistic effect or a weak synergistic effect between Tribulus terrestris and Yarrow.

[0111] In contrast, Example 5 showed an elastase inhibition rate of 32.05%, exceeding that of Example 3 and Comparative Example 5. This indicates that the excellent elastase inhibition rate achieved in Example 5 is not solely due to the synergistic effect between oregano leaf extract and tribulus fruit extract.

[0112] The above experiments also verified that the synergistic effect of oregano leaf extract and yarrow extract was weak when used together; the synergistic effect of tribulus fruit extract and yarrow extract was also weak when used together. Therefore, the reason why the elastase inhibitory ability of Example 5 was further enhanced compared with Example 3 and Comparative Example 5 may be that when oregano and tribulus are present at the same time, the synergistic effect of the two with yarrow extract is further enhanced, thereby further improving the elastase inhibitory ability of Example 5.

[0113] 3. DPPH free radical scavenging rate test

[0114] 3.1 Instruments and Equipment

[0115] BSA224S analytical balance, L6s ultraviolet spectrophotometer

[0116] 3.2 Reagents

[0117] DPPH (1,1-diphenyl-2-picrylhydrazine), 98%

[0118] 3.3 Test Methods

[0119] (1) Treatment of control materials and test samples

[0120] Sample group: Dilute the samples (compositions prepared in the examples and comparative examples) with pure water to a mass fraction of 5%;

[0121] Negative control: pure water.

[0122] (2) Experimental Operation Procedures

[0123] Set up a sample group and a negative control group, with three parallel tubes for each group. Add the corresponding reagent solution to each group, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solution of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0124] (3) The calculation formula is shown in Equation 2:

[0125]

[0126] Where: T—absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH;

[0127] T0—Sample background absorbance;

[0128] C-DPPH tube absorbance value, i.e., the absorbance value of DPPH solution without sample added;

[0129] C0 — Solvent background absorbance.

[0130] The test results are shown in Table 4:

[0131] Table 4: DPPH Free Radical Scavenging Rate Test Results

[0132]

[0133] Results analysis:

[0134] 1. As can be seen from Examples 1-3, when the composition is a mixture of oregano leaf extract and tribulus fruit extract, the DPPH free radical scavenging rate of the composition gradually increases with the increase of the proportion of oregano leaf extract in the composition;

[0135] Further observation of Example 4 shows that when yarrow extract is added to the composition, its DPPH free radical scavenging rate shows a further increasing trend compared to Example 3. At the same time, in Examples 5-6, when the mass ratio of oregano leaf extract, tribulus fruit extract and yarrow extract is 6:12:5, its DPPH free radical scavenging rate is more advantageous.

[0136] 2. As can be seen from Example 1 and Comparative Examples 1-2, when the composition contains only a single oregano leaf extract or tribulus fruit extract, the DPPH free radical scavenging rate of Comparative Example 1 is only 27.87%, and the elastase inhibition rate of Comparative Example 2 is only 23.90%, which is much lower than the 32.15% of Example 3.

[0137] It is evident that the oregano leaf extract and tribulus fruit extract produced a synergistic effect when used together, resulting in the composition prepared in Example 3 having a DPPH free radical scavenging capacity exceeding that of either Comparative Examples 1-2.

[0138] Further observation of Comparative Examples 3-5 revealed that when the composition of Comparative Example 3 was a mixture of oregano leaf extract and yarrow extract, its DPPH radical scavenging rate reached 26.98%. When Comparative Example 5 used yarrow extract alone, its DPPH radical scavenging rate was 24.35%. Further analysis of Comparative Example 1 showed that when Comparative Example 1 used oregano leaf extract alone, its DPPH radical scavenging rate was 27.87%. The elastase inhibition rate of Comparative Example 3 was between that of Comparative Example 1 and Comparative Example 5. Therefore, there may be no synergistic effect or a weak synergistic effect between oregano leaf extract and yarrow extract.

[0139] Similarly, comparing Comparative Examples 2, 4, and 5, it can be seen that the DPPH free radical scavenging rate of Comparative Example 4 is higher than that of Comparative Examples 2 and 5, indicating that there may be a certain synergistic effect between Tribulus terrestris and Yarrow.

[0140] Further observation of Example 5 reveals that its DPPH free radical scavenging rate reached 42.31%, exceeding that of Example 3 and Comparative Example 5. This indicates that the superior DPPH free radical scavenging rate of Example 5 is not solely due to the synergistic effect between oregano leaf extract and tribulus fruit extract. Furthermore, while Comparative Example 4 demonstrated a synergistic effect between tribulus and yarrow, its improvement compared to Comparative Example 5 was relatively small. Therefore, the superior DPPH free radical scavenging rate of Example 5 is more likely due to the enhanced synergistic effect of oregano and tribulus with yarrow extract when both are present, thus further improving the DPPH free radical scavenging ability of Example 5.

[0141] Application Example 1

[0142] An anti-wrinkle and firming raw material liquid, the formula of which is shown in Table 5:

[0143] Table 5: Formulation of Anti-wrinkle and Firming Raw Material Solution

[0144]

[0145]

[0146] A method for preparing an anti-wrinkle and firming raw material liquid:

[0147] Take a small amount of phase B (accounting for 20±5% of the total mass of phase B), add phase A to it, heat to 55-60℃ and stir until the solid is completely dissolved. After cooling, mix with the remaining phase B to obtain anti-wrinkle and firming raw material liquid.

[0148] Application Example 2

[0149] An emulsion, the formula of which is shown in Table 6:

[0150] Table 6: Emulsion Formulation Table

[0151]

[0152]

[0153] The preparation method of the above emulsion is as follows:

[0154] 1. Precast phase:

[0155] ① Phase A3 should be pre-stirred and dispersed evenly;

[0156] ②Preheat phase B1 to 75-80℃ and stir until completely dissolved, then keep at a constant temperature for later use;

[0157] ③ Heat phase C to 55-60℃ and stir until completely dissolved, then cool and set aside.

[0158] 2. Take a clean beaker, add phases A1, A2, and A3 at room temperature into the beaker, stir and heat to 85-88℃, keep warm for 20 minutes, homogenize appropriately for 1-2 minutes until there are no lumps, add the pretreated phase B1, stir evenly and homogenize for 2-3 minutes until a fine material is obtained, cool to 45-48℃, add phases C and D, stir and mix evenly to obtain an emulsion.

[0159] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle and firming composition, characterized in that, It includes oregano leaf extract and tribulus fruit extract, wherein the mass ratio of oregano leaf extract to tribulus fruit extract is 1-10:5-15.

2. The anti-wrinkle and firming composition according to claim 1, characterized in that, It also includes yarrow extract, wherein the mass ratio of oregano leaf extract, tribulus fruit extract and yarrow extract is 1-10:5-15:1-5.

3. The anti-wrinkle and firming composition according to claim 2, characterized in that, The extraction method of the yarrow extract is as follows: the yarrow is dried and crushed, then mixed with pure water, heated, stirred, centrifuged and concentrated to obtain an intermediate. Then, an equal volume of ethanol was added to the intermediate, the precipitate was removed after alcohol precipitation, the ethanol was evaporated to dryness and butylene glycol was added, followed by ultrafiltration and sterilization to obtain yarrow extract.

4. The anti-wrinkle and firming composition according to claim 1, characterized in that, The extraction method of the Tribulus terrestris fruit extract is as follows: the Tribulus terrestris fruit is dried and crushed, then mixed with pure water, heated, stirred, centrifuged, and concentrated to obtain an intermediate. Then, an equal volume of ethanol was added to the intermediate, the precipitate was removed after alcohol precipitation, the ethanol was evaporated to dryness, butanediol was added, ultrafiltration was performed, and sterilization was carried out to obtain Tribulus terrestris fruit extract.

5. The anti-wrinkle and firming composition according to claim 1, characterized in that, The extraction method of the oregano leaf extract is as follows: the oregano leaves are dried and crushed, then mixed with pure water, heated, stirred, centrifuged and concentrated to obtain an intermediate. Then, an equal volume of ethanol was added to the intermediate, the precipitate was removed after alcohol precipitation, the ethanol was evaporated to dryness and butylene glycol was added, followed by ultrafiltration and sterilization to obtain oregano leaf extract.

6. Use of the anti-wrinkle and firming composition according to any one of claims 1-5 in the preparation of cosmetics.

7. A cosmetic product, characterized in that, Containing 0.1 to 30 wt% of the anti-wrinkle and firming composition as described in any one of claims 1-5.

8. The cosmetic product according to claim 7, characterized in that, The dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion or cream.

9. The cosmetic product according to claim 7, characterized in that, The cosmetics mentioned are gels, serums, toners, lotions, creams, foundations, masks, facial cleansers, lotions, perfumes, makeup removers, lipsticks, blushes, or sprays.

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