Cell preparation and subpackaging system

Abstract

The invention relates to the technical field of biomedical treatment, and particularly provides a cell preparation and subpackaging system. Specifically, the cell preparation and subpackaging system comprises a base, a liquid separation assembly and a plurality of shaking devices, cryopreservation bags can be placed on the shaking devices, the liquid separation assembly can subpackage cell liquid into the multiple cryopreservation bags at first, then cryopreservation liquid is injected into each cryopreservation bag, the shaking devices can drive the cryopreservation bags to move in a reciprocating mode, and the cryopreservation bags are placed on the shaking devices. The shaking-up device is arranged on the base, so that cell sap in the cryopreservation bag is uniformly mixed with the cryopreservation liquid, a charging position is arranged on the base, and a charging device for charging the shaking-up device is arranged on the charging position. According to the method, the cell sap is subpackaged into the multiple cryopreservation bags, then the cryopreservation liquid is injected into each cryopreservation bag, due to the fact that the number of the cell sap in each cryopreservation bag is small, heat generated when the cryopreservation liquid is injected is small, and therefore cell burn can be avoided, and in the process that the cell sap and the cryopreservation liquid are shaken up, the cell sap and the cryopreservation liquid are shaken up, the cell sap and the cryopreservation liquid are shaken up. The shaking device is charged through the charging device, so that the use is more convenient.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically providing a cell preparation and dispensing system. Background Technology

[0002] During hematopoietic stem cell transplantation, the preparation of cryopreservation formulations involves injecting cryopreservation solution into a storage bag containing hematopoietic stem cells and continuously shaking the bag to ensure thorough mixing. The prepared cell solution is then aliquoted into multiple cryopreservation bags for cryopreservation. However, the injection of cryopreservation solution into the storage bags generates significant heat upon contact with the cell solution, potentially causing cell burns and resulting in low cell viability.

[0003] Therefore, a new technical solution is needed in this field to solve the above problems. Summary of the Invention

[0004] The present invention aims to solve the above-mentioned technical problems, namely, to solve the problem that existing cell preparation and packaging methods easily lead to cell burns.

[0005] In a first aspect, the present invention provides a cell preparation and dispensing system, including a base and a dispensing assembly and a plurality of shaking devices mounted on the base. The shaking devices are capable of holding cryopreservation bags. The dispensing assembly is configured to first dispense cell fluid into the cryopreservation bags on the plurality of shaking devices, and then inject cryopreservation solution into each cryopreservation bag on the shaking device. The shaking device is configured to drive the cryopreservation bags to reciprocate when the dispensing assembly injects cryopreservation solution into the cryopreservation bags, so as to mix the cell fluid in the cryopreservation bags with the cryopreservation solution. The base is provided with a charging position, and the charging position is provided with a charging device for charging the shaking devices.

[0006] In the preferred embodiment of the cell preparation and packaging system described above, a positioning and guiding mechanism is further provided on the charging position, which is used to position and guide the shaking device.

[0007] In the preferred embodiment of the cell preparation and dispensing system described above, the shaking device and the charging position are fixedly connected by magnetic attraction.

[0008] In the preferred embodiment of the cell preparation and packaging system described above, the charging device is a wireless charging device or a wired charging device.

[0009] In the preferred embodiment of the cell preparation and packaging system described above, the cell preparation and packaging system further includes a cooling component, which is used to cool the cryopreservation bag.

[0010] In the preferred embodiment of the cell preparation and packaging system described above, the cooling component is a water storage component, which can store cold water or ice water. The shaking device is fixedly installed in the water storage component, and the cryopreservation bag on the shaking device is located in the cold water or ice water.

[0011] In the preferred embodiment of the cell preparation and dispensing system described above, the shaking device includes a fixing component and a driving component mounted on the fixing component. The fixing component is fixedly connected to the water storage component. The driving component is provided with a receiving space for accommodating a cryopreservation bag. The driving component can drive the cryopreservation bag to move back and forth. The receiving space is provided with a snap-fit ​​structure that engages with the through-hole on the cryopreservation bag.

[0012] In the preferred embodiment of the cell preparation and packaging system described above, the driving component includes a driving mechanism and a moving component connected to the driving mechanism. The driving mechanism is capable of driving the moving component to reciprocate. The accommodating space is formed in the moving component. Alternatively, the accommodating space is configured to allow the cryopreservation bag to remain in a vertical or tilted state. Furthermore, the water storage component is provided with a fixed base, and the shaking device is magnetically connected to the fixed base. Finally, the bottom or side wall of the water storage component is provided with a drain outlet.

[0013] In the preferred embodiment of the cell preparation and dispensing system described above, the moving component includes a vertically arranged body connected to the driving mechanism. The body has a first limiting structure on its left side, a second limiting structure on its right side, and a third limiting structure at its bottom. The body, the first limiting structure, the second limiting structure, and the third limiting structure together form the accommodating space, and the top of the accommodating space is open. Furthermore, / or the driving mechanism can drive the moving component to move vertically up and down.

[0014] In the preferred embodiment of the cell preparation and dispensing system described above, the cell preparation and dispensing system further includes multiple gas-liquid separation bottles, which are respectively installed on the corresponding shaking device. The top of the gas-liquid separation bottle is connected to the dispensing assembly, and the bottom of the gas-liquid separation bottle is connected to the cryopreservation bag on the shaking device.

[0015] In the preferred embodiment of the cell preparation and dispensing system described above, the dispensing assembly includes a delivery pump, a first main pipeline, a second main pipeline, a first branch pipeline, a second branch pipeline, a third branch pipeline, a first control valve, a second control valve, and a third control valve.

[0016] The inlet and outlet of the delivery pump are connected to the first main pipeline and the second main pipeline, respectively. The first branch pipeline connects the first reservoir for storing cell fluid to the first main pipeline. The second branch pipeline connects the second reservoir for storing cryopreservation solution to the first main pipeline. There are multiple third branch pipelines. The cryopreservation bag on the shaking device is connected to the second main pipeline via the third branch pipelines.

[0017] The first control valve is located on the first branch pipe and is used to control the opening and closing of the first branch pipe. The second control valve is located on the second branch pipe and is used to control the opening and closing of the second branch pipe. There are multiple third control valves, each located on the third branch pipe and used to control the opening and closing of the third branch pipe.

[0018] With the above technical solution, the cell preparation and dispensing system of the present invention can first dispense cell fluid into multiple cryopreservation bags through the dispensing component, and then inject cryopreservation solution into each cryopreservation bag. Since the amount of cell fluid in each cryopreservation bag is relatively small, the heat generated when injecting cryopreservation solution is also relatively small, which helps to avoid cell burn. In addition, by setting a charging position on the base, when the battery power of the shaking device is insufficient, the shaking device can be placed on the charging position, and the battery of the shaking device can be charged through the charging device on the charging position, which is more convenient to use and helps to improve the user experience.

[0019] Furthermore, the shaking device of the present invention uses a positioning and guiding mechanism on the charging position of the base to position and guide the shaking device, which facilitates the accurate placement of the shaking device in the set position on the charging position, thereby enabling the charging device to be charged smoothly.

[0020] Furthermore, the shaking device of the present invention uses a magnetic attraction method to fix the shaking device to the charging position of the base, which makes it convenient to fix the shaking device to the charging position and to remove the shaking device from the charging position, making it more convenient to use.

[0021] Furthermore, the shaking device of the present invention is equipped with a cooling component. During the shaking process of the cell fluid and cryopreservation solution in the cryopreservation bag, the cooling component cools down the cryopreservation bag, which is more conducive to avoiding cell burn and greatly improving cell viability.

[0022] Furthermore, the present invention places the shaking device in a water storage component, which can store cold water or ice water. The cryopreservation bag is placed in the cold water or ice water and shaken. The cryopreservation bag is cooled by the cold water or ice water. The cold water or ice water is in direct contact with the cryopreservation bag, resulting in a better cooling effect and more effectively preventing cell burn.

[0023] Furthermore, the shaking device of the present invention has a snap-fit ​​structure in the receiving space, which can engage with the through hole on the cryopreservation bag to fix the cryopreservation bag in the receiving space. During the reciprocating movement of the cryopreservation bag driven by the driving component, the cryopreservation bag can be prevented from moving around in the receiving space, thereby further improving the mixing effect of cell fluid and cryopreservation solution and working efficiency.

[0024] Furthermore, the shaking device of the present invention sets the accommodating space to keep the cryopreservation bag in a vertical or inclined state. In this way, on the one hand, the resistance of the cryopreservation bag can be reduced, and on the other hand, it is easier to fix the cryopreservation bag, thereby improving the shaking effect.

[0025] Furthermore, the shaking device of the present invention, by setting the driving mechanism to be able to drive the moving member to move up and down in the vertical direction, so that the cryopreservation bag in the containing space is in a vertical or inclined state, makes it more advantageous to shake the cell fluid and cryopreservation solution in the cryopreservation bag evenly by moving the cryopreservation bag up and down in the vertical direction.

[0026] Furthermore, by incorporating a gas-liquid separation bottle, the present invention enables the venting operation to expel the gas from the cryopreservation bag.

[0027] Furthermore, the shaking device of the present invention is fixedly connected to the fixed seat in the water storage component by means of magnetic attraction, which facilitates the fixing of the shaking device and prevents the shaking device from tipping over during operation. Attached Figure Description

[0028] The preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:

[0029] Figure 1 This is a schematic diagram of the cell dispensing system of the present invention. Figure 1 ;

[0030] Figure 2 This is a schematic diagram of the cell dispensing system of the present invention. Figure 2 ;

[0031] Figure 3 This is a schematic diagram of the cell dispensing system of the present invention. Figure 3 ;

[0032] Figure 4 This is a schematic diagram of the structure of a cryopreservation bag;

[0033] Figure 5 This is a schematic diagram of the structure of the shaking device in the cell dispensing system of the present invention. Figure 1 ;

[0034] Figure 6 This is a schematic diagram of the structure of the shaking device in the cell dispensing system of the present invention. Figure 2 ;

[0035] Figure 7 This is a schematic diagram of the structure of the shaking device in the cell dispensing system of the present invention. Figure 3 ;

[0036] Figure 8 This is a schematic diagram of the structure of the moving component of the shaking device of the present invention;

[0037] Figure 9 This is a schematic diagram of the structure of the fixing component and the driving mechanism of the shaking device of the present invention;

[0038] Figure 10 This is a schematic diagram of the drive mechanism of the shaking device of the present invention.

[0039] List of reference numerals in the attached diagram:

[0040] 1. Liquid dispensing assembly; 11. Transfer pump; 12. First main pipeline; 13. Second main pipeline; 14. First branch pipeline; 15. Second branch pipeline; 16. Third branch pipeline; 17. First control valve; 18. Second control valve; 19. Third control valve; 10. Fourth control valve; 2. Shaking device; 21. Fixing component; 210. Front panel; 211. Handle; 212. First magnet; 214. Back plate; 215. Wireless charging receiver; 216. Third magnet; 22. Moving component; 221. Body; 222. First limiting structure; 223. Second limiting structure; 224. Third limiting structure; 225. Snap-fit ​​structure; 2211. First weight reduction hole; 2221. First U-shaped limiting part; 222 2. First vertical limiting part; 2231. Second U-shaped limiting part; 2232. Second vertical limiting part; 2241. Fourth weight reduction hole; 22221. Second weight reduction hole; 22321. Third weight reduction hole; 23. Drive mechanism; 231. Motor; 232. Crank; 233. Connecting rod; 234. Sliding seat; 235. Guide rail; 236. Slider; 3. Water storage component; 31. Fixed seat; 32. Drain outlet; 33. Second magnet; 4. Gas-liquid separation bottle; 5. Cryopreservation bag; 51. Through hole; 61. First liquid storage component; 62. Second liquid storage component; 63. Plasma bag; 7. Base; 71. Central control screen; 72. Charging position; 73. Positioning guide mechanism; 74. Wireless charging transmitter; 75. Fourth magnet. Detailed Implementation

[0041] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0042] It should be noted that in the description of this invention, terms such as "inner" and "outer," which indicate direction or positional relationship, are based on the direction or positional relationship shown in the accompanying drawings. This is merely for ease of description and does not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0043] Furthermore, it should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "set," "connect," and "install" should be interpreted broadly, for example, referring to a fixed connection, a detachable connection, or an integral connection. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0044] Specifically, the present invention provides a cell preparation and dispensing system, such as... Figures 1 to 5 As shown, the cell preparation and dispensing system of the present invention includes a base 7 and a dispensing assembly 1 and a plurality of shaking devices 2 mounted on the base 7. Cryopreservation bags 5 can be placed on the shaking devices 2. The dispensing assembly 1 is configured to first dispense cell fluid into the cryopreservation bags 5 on the plurality of shaking devices 2, and then inject cryopreservation solution into the cryopreservation bags 5 on each shaking device 2. The shaking devices 2 are configured to drive the cryopreservation bags 5 to reciprocate when the dispensing assembly 1 injects cryopreservation solution into the cryopreservation bags 5, so that the cell fluid in the cryopreservation bags 5 is mixed with the cryopreservation solution.

[0045] Compared to the existing technology that mixes cell fluid with cryopreservation solution first and then dispenses the prepared cell fluid into multiple cryopreservation bags 5, this invention dispenses cell fluid into multiple cryopreservation bags 5 first, and then injects cryopreservation solution into each cryopreservation bag 5. Since the amount of cell fluid in each cryopreservation bag 5 is relatively small, the heat generated when injecting cryopreservation solution is also relatively small, which helps to avoid cell burn.

[0046] Preferably, such as Figure 1 and Figure 2 As shown, the cell preparation and dispensing system of the present invention also includes a cooling component for cooling the cryopreservation bag 5.

[0047] The cell preparation and dispensing system of the present invention, by setting a cooling component, cools down the cryopreservation bag 5 during the process of shaking the cell solution and cryopreservation solution in the cryopreservation bag 5, which is more conducive to avoiding cell burn and greatly improving cell viability.

[0048] Exemplarily, the dispensing component 1, cooling component, and shaking device 2 of the cell preparation and dispensing system of the present invention are all mounted on the base 7. The cell fluid is stored in the first storage component 61, and the cryopreservation solution is stored in the second storage component 62. There are six shaking devices 2, and each shaking device 2 has a cryopreservation bag 5. The inlet end of the dispensing component 1 is connected to the first storage component 61 and the second storage component 62, and the outlet end of the dispensing component 1 is connected to the cryopreservation bags 5 on the six shaking devices 2. The dispensing component 1 first distributes the cell fluid in the first storage component 61 evenly into the six cryopreservation bags 5, and then injects the cryopreservation solution in the second storage component 62 into the six cryopreservation bags 5 in a predetermined order. The ratio of cell fluid to cryopreservation solution is 1:1. During the process of injecting cryopreservation solution into the cryopreservation bags 5, the shaking device 2 moves back and forth with the cryopreservation bags 5 to shake the cell fluid and cryopreservation solution in the cryopreservation bags 5 evenly.

[0049] It should be noted that the number of shaking devices 2 is not limited to the six mentioned above. Those skilled in the art can flexibly set the specific number of shaking devices 2 according to specific needs in practical applications. For example, the number of shaking devices 2 can be set to three, four, eight or more.

[0050] In addition, it should be noted that the first liquid storage component 61 and the second liquid storage component 62 can be configured as a liquid storage bag or a liquid storage bottle. Of course, it is preferred to configure the first liquid storage component 61 and the second liquid storage component 62 as a liquid storage bag.

[0051] Furthermore, it should be noted that the present invention does not limit the specific type of cooling component. For example, the cooling component can be set as a water-cooled component, an air-cooled component, or a semiconductor cooling component, etc. Such adjustments and changes to the specific type of cooling component do not deviate from the principles and scope of the present invention and should be limited to the protection scope of the present invention.

[0052] Preferably, such as Figure 1 and Figure 2 As shown, the cooling component of the present invention is a water storage component 3, which can store cold water or ice water. The shaking device 2 is installed in the water storage component 3, and the cryopreservation bag 5 on the shaking device 2 is located in the cold water or ice water.

[0053] Here, "ice water" refers to a mixture of water and ice, with a temperature of 0°C. Cold water has a slightly higher temperature than ice water. Preferably, the cold water temperature is limited to no more than 15°C, more preferably no more than 8°C, and most preferably no more than 4°C. Of course, ice water is more preferable than cold water for its better cooling effect. Furthermore, it should be noted that the cryopreservation bags are preferably completely immersed in cold or ice water and shaken well.

[0054] By placing the shaking device 2 into the water storage component 3, the staff can add cold water or ice water to the water storage component 3, place the cryopreservation bag 5 in the cold water or ice water and shake it. The cold water or ice water cools down the cryopreservation bag 5. The cold water or ice water comes into direct contact with the cryopreservation bag 5, resulting in a better cooling effect and more effectively preventing cell burns.

[0055] Preferably, such as Figure 1 and Figure 3 As shown, the liquid dispensing component 1 of the present invention can also be connected to the plasma bag 63 to deliver the plasma located above the cell fluid into the plasma bag 63.

[0056] The original blood needs to be centrifuged first to separate the blood into layers, with plasma on the top layer and blood cells on the bottom layer. Before the blood cells are separated, the plasma on the top layer needs to be separated out. The liquid separation component 1 of the present invention can first transport the plasma in the first liquid storage component 61 to the plasma bag 63, and then separate the blood cells in the first liquid storage component 61 into multiple cryopreservation bags 5.

[0057] Preferably, such as Figure 1 and Figure 3 As shown, the cell preparation and dispensing system of the present invention also includes a first weighing sensor (not shown in the figure), which can be connected to the plasma bag 63.

[0058] By connecting the first weighing sensor to the plasma bag 63, the weight of the plasma bag 63 can be detected, thereby determining the amount of plasma in the plasma bag 63.

[0059] For example, the cell preparation and dispensing system of the present invention has a receiving groove for placing a plasma bag 63 on the base 7. A first weighing sensor is disposed in the receiving groove. The first weighing sensor is a piezoelectric sensor. The plasma bag 63 is pressed on the first weighing sensor. The first weighing sensor is communicatively connected to the controller of the cell preparation and dispensing system and can transmit the detected pressure data to the controller. The controller can determine the amount of plasma in the plasma bag 63 based on the acquired pressure data.

[0060] It should be noted that the cell preparation and dispensing system of the present invention may also include a centrifuge, which is used to centrifuge the original blood in the first liquid storage component 61 to separate the plasma and blood cells.

[0061] Preferably, such as Figure 1 and Figure 3 As shown, the liquid separation assembly 1 of the present invention includes a transfer pump 11, a first main pipeline 12, a second main pipeline 13, a first branch pipeline 14, a second branch pipeline 15, a third branch pipeline 16, a first control valve 17, a second control valve 18, and a third control valve 19.

[0062] The inlet and outlet of the delivery pump 11 are connected to the first main pipeline 12 and the second main pipeline 13, respectively. The first branch pipeline 14 is used to connect the first liquid storage component 61 storing cell fluid to the first main pipeline 12. The second branch pipeline 15 is used to connect the second liquid storage component 62 storing cryopreservation solution to the first main pipeline 12. There are multiple third branch pipelines 16. The cryopreservation bag 5 on the shaking device 2 is connected to the second main pipeline 13 through the third branch pipeline 16. The first control valve 17 is located on the first branch pipeline 14 and is used to control the opening and closing of the first branch pipeline 14. The second control valve 18 is located on the second branch pipeline 15 and is used to control the opening and closing of the second branch pipeline 15. There are multiple third control valves 19. The third control valve 19 is located on the third branch pipeline 16 and is used to control the opening and closing of the third branch pipeline 16.

[0063] For example, the water storage component 3 is equipped with six shaking devices 2, each of which has a cryopreservation bag 5. The third branch pipe 16 and the third control valve 19 are also six in number. First, the first control valve 17 and one of the third control valves 19 are opened, and the delivery pump 11 is started to inject the cell fluid in the first liquid storage component 61 into a cryopreservation bag 5. When the amount of injected cell fluid reaches the set amount, the third control valve 19 is closed, and another third control valve 19 is opened in the set order to inject the same amount of cell fluid into another cryopreservation bag 5. This continues until the cell fluid in the first liquid storage component 61 is distributed into six cryopreservation bags 5. Then, the first control valve 17 is closed, the second control valve 18 is opened, and cryopreservation solution is injected into the six cryopreservation bags 5 in the order of cell fluid injection.

[0064] The delivery pump 11, the first control valve 17, the second control valve 18, and the third control valve 19 are all connected to the controller of the cell preparation and dispensing system. The controller can control the delivery pump 11, the first control valve 17, the second control valve 18, and the third control valve 19. The delivery pump 11 is preferably a peristaltic pump, the first branch pipe 14, the second branch pipe 15, and the third branch pipe 16 are preferably flexible hoses, and the first control valve 17, the second control valve 18, and the third control valve 19 are preferably clamp valves.

[0065] It should be noted that, in practical applications, those skilled in the art can connect the end of the second main pipeline 13 to the plasma bag 63, and set a fourth control valve 10 near the plasma bag 63 to control the on / off state of the second main pipeline 13. During the plasma separation process, the first control valve 17 and the fourth control valve 10 are opened, the third control valve 19 is completely closed, the transfer pump 11 is started, and the plasma in the first liquid storage component 61 is transferred to the plasma bag 63. After the transfer is completed, the fourth control valve 10 is closed, and then the third control valve 19 is opened one by one to perform liquid separation.

[0066] Preferably, such as Figure 1 and Figure 3 As shown, the cell preparation and dispensing system of the present invention also includes multiple gas-liquid separation bottles 4, which are respectively installed on corresponding shaking devices 2. The top of the gas-liquid separation bottle 4 is connected to the liquid dispensing component 1, and the bottom of the gas-liquid separation bottle 4 is connected to the cryopreservation bag 5 on the shaking device 2.

[0067] For example, each shaking device 2 is equipped with a gas-liquid separation bottle 4, which is installed on top of the shaking device 2, above the cryopreservation bag 5. The bottom end of the gas-liquid separation bottle 4 is connected to the cryopreservation bag 5 through a third branch pipe 16, and the top end of the gas-liquid separation bottle 4 is connected to the second main pipe 13. When cell fluid is injected into the cryopreservation bag 5, the cell fluid in the second main pipe 13 first enters the gas-liquid separation bottle 4, and then enters the cryopreservation bag 5 through the third branch pipe 16 below the gas-liquid separation bottle 4. The cryopreservation solution also first enters the gas-liquid separation bottle 4. The separation bottle 4 is then placed into the cryopreservation bag 5. After the cell solution and cryopreservation solution in the cryopreservation bag 5 are shaken well, the gas in the cryopreservation bag 5 needs to be vented. Specifically, the transfer pump 11 is first reversed to draw all the prepared cell solution in the cryopreservation bag 5 into the gas-liquid separation bottle 4, while the gas in the cryopreservation bag 5 is vented. The cell solution is located at the bottom of the gas-liquid separation bottle 4, and all the air is located above the cell solution. Then the transfer pump 11 is reversed to inject the cell solution in the gas-liquid separation bottle 4 into the cryopreservation bag 5 to achieve gas-liquid separation and complete the venting operation.

[0068] It should be noted that a central control screen 71 is installed on the base 7. The staff can control the delivery pump 11 and various control valves through the central control screen 71, and can also set parameters through the central control screen 71. The above-mentioned exhaust operation can be manually controlled by the staff through the central control screen 71.

[0069] Preferably, such as Figure 1 As shown, the cell preparation and dispensing system of the present invention further includes a second weighing sensor (not shown in the figure), which is connected to a first liquid storage component 61 for storing cell fluid.

[0070] By connecting a second weighing sensor to the first liquid storage component 61, the weight of the cell fluid in the first liquid storage component 61 can be obtained, thereby determining the weight of the cell fluid injected into the cryopreservation bag 5. Specifically, the weight reduction of the first liquid storage component 61 during the separation operation is the weight of the cell fluid injected into the cryopreservation bag 5. For example, the first liquid storage component 61 is attached to the second weighing sensor, which is a piezoelectric sensor.

[0071] In addition, by setting a second weighing sensor connected to the first liquid storage component 61, the weight of the remaining blood in the first liquid storage component 61 can be obtained during the process of extracting plasma. When the weight of the remaining blood reaches the set weight, the plasma extraction is stopped.

[0072] It should be noted that, in addition to determining the amount of cell fluid injected into the cryopreservation bag 5 based on the detection data of the second weighing sensor, the amount of cell fluid injected into the cryopreservation bag 5 can also be determined based on the flow rate and time of the delivery pump 11.

[0073] Preferably, such as Figure 1 As shown, the cell preparation and dispensing system of the present invention also includes a third weighing sensor (not shown in the figure), which is connected to a second liquid storage component 62 for storing cryopreservation solution.

[0074] By connecting a third weighing sensor to the second liquid storage component 62, the weight of the cryopreservation liquid in the second liquid storage component 62 can be obtained, thereby determining the weight of the cryopreservation liquid injected into the cryopreservation bag 5.

[0075] For example, the cell preparation and dispensing system of the present invention has a receiving groove on the base 7 for placing the second liquid storage component 62. The third weighing sensor is disposed in the receiving groove. The third weighing sensor is a piezoelectric sensor. The second liquid storage component 62 is pressed on the third weighing sensor. The third weighing sensor is communicatively connected to the controller of the cell preparation and dispensing system and can transmit the detected pressure data to the controller. The controller can determine the amount of cryopreservation liquid in the second liquid storage component 62 based on the acquired pressure data.

[0076] Preferably, such as Figures 1 to 8 As shown, the shaking device 2 of the present invention includes a fixing member 21 and a driving component installed on the fixing member 21. The fixing member 21 is fixedly connected to the water storage member 3. The driving component is provided with a receiving space for accommodating the cryopreservation bag 5. The driving component can drive the cryopreservation bag 5 to move back and forth.

[0077] Staff can place the cryopreservation bag 5 into the receiving space of the shaking device 2, first inject the cell fluid into the cryopreservation bag 5, and then inject the cryopreservation solution into the cryopreservation bag 5. During the process of injecting the cryopreservation solution into the cryopreservation bag 5, the drive component drives the cryopreservation bag 5 to move back and forth so that the injected cryopreservation solution and cell fluid are mixed evenly.

[0078] For example, the containment space extends vertically and is entirely located in cold or ice water. The containment space enables the cryopreservation bag 5 to remain vertical. The cell solution and cryopreservation solution are both located at the bottom of the cryopreservation bag 5, which is also entirely located in cold or ice water.

[0079] It should be noted that, in practical applications, those skilled in the art may also tilt the containing space to keep the cryopreservation bag 5 tilted, or extend the containing space horizontally to keep the cryopreservation bag 5 horizontal, etc. Such flexible adjustments and changes do not deviate from the principles and scope of the present invention and should all be limited to the protection scope of the present invention.

[0080] Of course, the present invention preferably arranges the containing space in a way that allows the cryopreservation bag 5 to remain in a vertical or tilted state, but the tilt angle should not be too large, and the angle between the containing space and the vertical direction is preferably no greater than 20°. Most preferably, the present invention extends the containing space along the vertical direction, thus ensuring that the cryopreservation bag 5 remains in a vertical state.

[0081] Preferably, such as Figure 2 As shown, a drain outlet 32 ​​is provided on the side wall of the water storage component 3. Exemplarily, the water storage component 3 is a rectangular water tank with an open top. A drain outlet 32 ​​is provided on the side wall of the water tank, and a drain valve is installed at the drain outlet 32. When the temperature of the water in the tank rises, ice can be added to the tank to lower the water temperature. When the water level in the tank is relatively high, the drain valve is opened to allow the water in the tank to drain out from the drain outlet 32.

[0082] It should be noted that, in practical applications, those skilled in the art can also place the drain outlet 32 ​​on the bottom wall of the water storage component 3. Of course, the present invention preferably places the drain outlet 32 ​​on the side wall of the water storage component 3 to facilitate drainage operations.

[0083] Preferably, such as Figures 5 to 7 As shown, the top of the fixing member 21 of the shaking device 2 of the present invention is provided with a handle 211. By providing a handle 211 at the top of the fixing member 21, it is convenient for the operator to lift the shaking device 2.

[0084] The fixing component 21 is preferably a housing, and the handle 211 is installed on the top surface of the housing. The handle 211 is approximately a U-shaped structure.

[0085] Preferably, such as Figure 2 and Figure 5 As shown, a fixing seat 31 is provided in the water storage component 3, and the fixing component 21 and the fixing seat 31 are fixedly connected by magnetic attraction.

[0086] For example, four first magnets 212 are provided on the bottom surface of the fixing member 21. The four first magnets 212 are roughly arranged in a rectangle or square. Four second magnets 33 are also provided on the fixing base 31. After the shaking device 2 is placed on the fixing base 31, the four first magnets 212 and the four second magnets 33 attract each other to fix the shaking device 2 to the fixing base 31, so as to prevent the shaking device 2 from tipping over during operation.

[0087] It should be noted that the magnetic attraction method is not limited to the magnetic attraction combination of magnets mentioned above. For example, it can also be a magnetic attraction combination of a magnet and an iron component (such as an iron block or iron plate), a magnetic attraction combination of a magnet and an electromagnet, or a magnetic attraction combination of an electromagnet and an iron component. Such flexible adjustments and changes do not deviate from the principles and scope of this invention and should all be limited to the protection scope of this invention. Of course, this invention preferably uses a magnetic attraction combination of magnets.

[0088] Furthermore, it should be noted that the present invention preferably provides multiple fixing seats 31 in the water storage component 3, and each fixing seat 31 can fix a shaking device 2.

[0089] Preferably, such as Figures 4 to 8 As shown, the shaking device 2 of the present invention is provided with a snap-fit ​​structure 225 in the accommodating space. The snap-fit ​​structure 225 is snapped into the through hole 51 on the cryopreservation bag 5, and the driving component can drive the cryopreservation bag 5 to move back and forth.

[0090] By setting a snap-fit ​​structure 225 in the containment space to snap into the through hole 51 on the cryopreservation bag 5, the cryopreservation bag 5 can be fixed in the containment space. During the process of the drive component driving the cryopreservation bag 5 to move back and forth, the cryopreservation bag 5 can be prevented from moving around in the containment space, thereby improving the mixing effect of cell fluid and cryopreservation solution and working efficiency.

[0091] For example, the cryopreservation bag 5 is placed vertically into the receiving space of the shaking device 2. Two elongated through holes 51 are provided near the bottom of the cryopreservation bag 5. The two through holes 51 extend in the left and right direction and are spaced apart in the left and right direction. The snap-fit ​​structure 225 provided in the receiving space includes two elongated snap-fit ​​posts. The snap-fit ​​posts also extend in the left and right direction, and the length of the snap-fit ​​posts is greater than the length of the through holes 51. The snap-fit ​​posts can pass through the through holes 51 and snap-fit ​​with the through holes 51.

[0092] It should be noted that the present invention is not limited to setting the snap-fit ​​structure 225 as a snap-fit ​​post. For example, it can also be set as a snap-fit ​​plate or a snap-fit ​​claw, as long as it can snap-fit ​​with the through hole 51 on the cryopreservation bag 5.

[0093] Furthermore, it should be noted that, in practical applications, those skilled in the art can configure the driving component to drive the cryopreservation bag 5 to move up and down in the vertical direction, or to drive the cryopreservation bag 5 to move left and right in the horizontal direction, or to drive the cryopreservation bag 5 to move back and forth along an elliptical trajectory, etc. Such flexible adjustments and changes do not deviate from the principles and scope of the present invention and should all be limited to the protection scope of the present invention.

[0094] Preferably, such as Figures 5 to 10 As shown, the drive assembly of the present invention includes a drive mechanism 23 and a movable member 22 connected to the drive mechanism 23. The drive mechanism 23 can drive the movable member 22 to reciprocate, and a receiving space is formed in the movable member 22.

[0095] In other words, the cryopreservation bag 5 is placed on the moving component 22, and the moving component 22 is driven to move back and forth by the driving mechanism 23, thereby moving the cryopreservation bag 5 on the moving component 22 back and forth. The snap-fit ​​structure 225 is also provided on the moving component 22 to secure the cryopreservation bag 5 to the moving component 22.

[0096] Preferably, such as Figures 5 to 10 As shown, the drive mechanism 23 of the present invention is configured to drive the moving member 22 to move up and down in the vertical direction. The cryopreservation bag 5 located in the containing space is in a vertical or inclined state. By moving the cryopreservation bag 5 up and down in the vertical direction, it is more advantageous to shake the cell fluid and cryopreservation solution in the cryopreservation bag 5 evenly.

[0097] Preferably, such as Figures 5 to 8 As shown, the movable component 22 of the present invention includes a vertically arranged body 221, which is connected to the drive mechanism 23. A first limiting structure 222 is provided on the left side of the body 221, a second limiting structure 223 is provided on the right side of the body 221, and a third limiting structure 224 is provided at the bottom of the body 221. The body 221, the first limiting structure 222, the second limiting structure 223 and the third limiting structure 224 together form an accommodating space, and the top of the accommodating space is open.

[0098] The cryopreservation bag 5 can be inserted into the storage space from the top, making it easier to operate. The third limiting structure 224 supports the bottom of the cryopreservation bag 5. The first limiting structure 222 and the second limiting structure 223 are set opposite to each other. The first limiting structure 222 and the second limiting structure 223 abut against the left and right sides of the cryopreservation bag 5, or there may be a slight gap, but the size of the gap is preferably no more than 0.5mm. The snap-fit ​​structure 225 in the storage space snaps into the through hole 51 on the cryopreservation bag 5 to prevent the cryopreservation bag 5 from moving up and down in the storage space.

[0099] Preferably, such as Figures 5 to 8As shown, the body 221 of the movable component 22 is a plate-shaped body 221.

[0100] Preferably, such as Figures 5 to 8 As shown, the first limiting structure 222 includes a first U-shaped limiting part 2221 and a first vertical limiting part 2222. The opening of the first U-shaped limiting part 2221 faces the right side of the body 221 of the moving member 22. One end of the first U-shaped limiting part 2221 is fixedly connected to or integrally formed with the left edge of the body 221. The other end of the first U-shaped limiting part 2221 is fixedly connected to or integrally formed with the left edge of the first vertical limiting part 2222. The first vertical limiting part 2222 extends from the other end of the first U-shaped limiting part 2221 toward the right side of the body 221.

[0101] The first U-shaped limiting part 2221 has a U-shaped cross-section, and the first vertical limiting part 2222 is preferably arranged parallel to the body 221 of the moving member 22. The left side of the cryopreservation bag 5 is located between the body 221 of the moving member 22 and the first vertical limiting part 2222. The gap between the body 221 of the moving member 22 and the first vertical limiting part 2222 is substantially the same as the thickness of the cryopreservation bag 5.

[0102] Preferably, such as Figures 5 to 8 As shown, the second limiting structure 223 includes a second U-shaped limiting part 2231 and a second vertical limiting part 2232. The opening of the second U-shaped limiting part 2231 faces the left side of the second U-shaped limiting part 2231. One end of the second U-shaped limiting part 2231 is fixedly connected to or integrally formed with the right side edge of the body 221. The other end of the second U-shaped limiting part 2231 is fixedly connected to or integrally formed with the right side edge of the second vertical limiting part 2232. The second vertical limiting part 2232 extends from the other end of the second U-shaped limiting part 2231 toward the left side of the body 221.

[0103] The second U-shaped limiting part 2231 has a U-shaped cross-section, and the second vertical limiting part 2232 is preferably arranged parallel to the body 221 of the moving member 22. The right side of the cryopreservation bag 5 is located between the body 221 and the second vertical limiting part 2232. The gap between the body 221 and the second vertical limiting part 2232 of the moving member 22 is basically the same as the thickness of the cryopreservation bag 5.

[0104] Preferably, such as Figures 5 to 8As shown, the right edge of the first vertical limiting part 2222 and the left edge of the second vertical limiting part 2232 on the moving member 22 are separated by a gap. That is, the first vertical limiting part 2222 and the second vertical limiting part 2232 are not in contact, and there is a gap between them. This allows for a reduction in the size of the first vertical limiting part 2222 and the second vertical limiting part 2232, thereby reducing costs and the weight of the moving member 22, making it easier to drive its reciprocating movement.

[0105] Preferably, such as Figures 5 to 8 As shown, the main body 221, the first vertical limiting part 2222, the second vertical limiting part 2232, and the third limiting structure 224 of the moving member 22 are all provided with weight-reducing holes. By providing weight-reducing holes on the moving member 22, the weight of the moving member 22 can be further reduced.

[0106] For example, two first weight-reducing holes 2211 are provided on the body 221 of the moving member 22, a second weight-reducing hole 22221 is provided on the first vertical limiting part 2222, a third weight-reducing hole 22321 is provided on the second vertical limiting part 2232, and a fourth weight-reducing hole 2241 is provided on the third limiting structure 224.

[0107] It should be noted that, in practical applications, those skilled in the art may provide weight-reducing holes only on any one of the main body 221, the first vertical limiting part 2222, the second vertical limiting part 2232, and the third limiting structure 224 of the moving member 22; or weight-reducing holes may be provided on any two of the main body 221, the first vertical limiting part 2222, the second vertical limiting part 2232, and the third limiting structure 224 of the moving member 22; or weight-reducing holes may be provided on any three of the main body 221, the first vertical limiting part 2222, the second vertical limiting part 2232, and the third limiting structure 224 of the moving member 22. Such flexible adjustments and changes do not deviate from the principles and scope of the present invention and should all be limited to the protection scope of the present invention.

[0108] Preferably, such as Figure 9 and Figure 10As shown, the drive mechanism 23 of the present invention includes a motor 231, a crank 232, a connecting rod 233, a sliding seat 234, and a guide rail 235 extending in the vertical direction. The motor 231 is installed inside the housing and is located near the top of the housing. The guide rail 235 is installed on the front panel 210 of the housing. The drive shaft of the motor 231 passes through the front panel 210 and is fixedly connected to one end of the crank 232. The other end of the crank 232 is pivotally connected to one end of the connecting rod 233. The other end of the connecting rod 233 is pivotally connected to the sliding seat 234. The sliding seat 234 is slidably engaged with the guide rail 235 and can move along the length direction of the guide rail 235. The moving member 22 is fixedly connected to the sliding seat 234. The motor 231 and the guide rail 235 are fixedly connected to the fixing member 21 of the shaking device 2.

[0109] It should be noted that, in order to prevent water in the water storage component 3 from seeping into the motor 231, the present invention also preferably incorporates a waterproof design for the motor 231. For example, a sealing ring is installed on the drive shaft of the motor 231. This sealing ring is preferably a wear-resistant Glyd ring with rotational waterproofing function. A motor mounting seat is provided on the inner side wall of the front panel 210, and a sealing ring is also provided between the motor mounting seat and the front panel 210, forming a multi-level waterproofing, which can very effectively prevent water from entering the motor 231.

[0110] For example, the motor 231 is located above the sliding seat 234, and the connecting rod 233 is set approximately vertically. The motor 231 drives the sliding seat 234 to move up and down along the guide rail 235 through the crank-connecting rod mechanism composed of the crank 232 and the connecting rod 233. The sliding seat 234 moves up and down with the moving component 22 and the cryopreservation bag 5 on the moving component 22.

[0111] For example, such as Figure 6 and Figure 9 As shown, the shell is a cuboid structure, mainly consisting of a main shell and a back plate 214. The main shell is formed by a front panel 210, a bottom plate, a top plate, and two side plates. To prevent water from the water storage component 3 from seeping into the shell, the front panel, bottom plate, top plate, and two side plates are integrally formed. The back plate 214 is detachably fixed to the main shell. The main shell has an annular groove on the side facing the back plate 214. A sealing ring is installed in the annular groove. The sealing ring is sealed and fitted with the back plate 214, sealing the gap between the back plate 214 and the main shell to prevent water from seeping into the shell.

[0112] Preferably, such as Figure 9 and Figure 10 As shown, the sliding seat 234 of the drive mechanism 23 of the present invention is provided with a slider 236 at a position corresponding to the guide rail 235, and the slider 236 slides in cooperation with the guide rail 235.

[0113] For example, the outer side of the sliding seat 234 is fixedly connected to the body 221 of the moving member 22, the slider 236 is disposed on the inner side of the sliding seat 234, the guide rail 235 is provided with a guide groove that can accommodate the slider 236, and the left and right side walls of the guide groove are provided with opposing limiting grooves. The left and right ends of the slider 236 are respectively inserted into the corresponding limiting grooves, so that the slider 236 can only move up and down in the length direction of the guide groove.

[0114] Preferably, the slider 236 is made of self-lubricating engineering plastic. By using self-lubricating engineering plastic to make the slider 236, the frictional resistance between the slider 236 and the guide rail 235 can be reduced, allowing the slider 236 to slide up and down along the guide rail 235 more smoothly.

[0115] Preferably, such as Figure 9 and Figure 10 As shown, there are two guide rails 235, which are spaced apart in the left and right direction, and there are two sliders 236, which slide in cooperation with the corresponding guide rails 235.

[0116] By setting two guide rails 235 spaced apart on the fixed component 21, and setting two sliders 236 on the sliding seat 234 to slide and engage with the two guide rails 235 respectively, the sliding seat 234 can move up and down more stably.

[0117] Preferably, such as Figure 1 and Figure 2 As shown, a charging position 72 is provided on the base 7 of the cell preparation and dispensing system of the present invention, and a charging device for charging the shaking device 2 is provided on the charging position 72.

[0118] By setting a charging position 72 on the base 7, when the battery of the shaking device 2 (the battery is mainly used to power the motor of the drive component) is low on power, the shaking device 2 can be placed on the charging position 72, and the battery of the shaking device 2 can be charged through the charging device on the charging position 72, which is more convenient to use and helps to improve the user experience.

[0119] For example, such as Figure 2 and Figure 6 As shown, the water storage component 3 has six fixed seats 31, and the base 7 has six charging positions 72. Each charging position 72 is equipped with a wireless charging transmitter 74. The number of shaking devices 2 is six or twelve, and each shaking device 2 is equipped with a wireless charging receiver 215. The wireless charging transmitters 74 and wireless charging receivers 215 cooperate to charge the battery of the shaking device 2. It should be noted that the charging device of the present invention is not limited to the wireless charging device described above. In practical applications, those skilled in the art can also configure the charging device as a wired charging device.

[0120] Preferably, such as Figure 2 and Figure 6 As shown, the shaking device 2 of the present invention is fixedly connected to the charging position 72 on the base 7 by magnetic attraction.

[0121] By using magnetic attraction to fix the shaking device 2 to the charging position 72 of the base 7, it is convenient to fix the shaking device 2 to the charging position 72 and to remove the shaking device 2 from the charging position 72, making it more convenient to use.

[0122] For example, two third magnets 216 are installed on the back plate 214 of the housing of the shaking device 2. The two third magnets 216 are distributed at intervals in the vertical direction. The wireless charging receiver 215 is also installed on the back plate 214 and is located between the two third magnets 216. The charging position 72 includes a horizontal support surface and a vertical surface. Two fourth magnets 75 are installed on the vertical surface and are distributed at intervals in the vertical direction. The wireless charging transmitter 74 is also installed on the vertical surface and is located between the two fourth magnets 75. When the shaking device 2 is placed on the horizontal support surface of the charging position 72, the third magnets 216 on the shaking device 2 attract the fourth magnets 75 on the charging position 72, thereby fixing the shaking device 2 on the charging position 72 and preventing the shaking device 2 from tipping over.

[0123] It should be noted that the number of the third magnet 216 and the fourth magnet 75 is not limited to the two mentioned above; for example, it can also be set to one or three, etc. Furthermore, the magnetic attraction method is not limited to the magnetic attraction combination of magnets. For example, it can also be set to a magnetic attraction combination of a magnet and an iron component (such as an iron block or iron plate), a magnetic attraction combination of a magnet and an electromagnet, or a magnetic attraction combination of an electromagnet and an iron component. Such flexible adjustments and changes do not deviate from the principles and scope of the present invention and should all be limited within the protection scope of the present invention. Of course, the present invention preferably adopts a magnetic attraction combination of magnets.

[0124] Preferably, such as Figure 2 and Figure 6 As shown, the charging position 72 on the base 7 of the present invention is also provided with a positioning guide mechanism 73, which is used to position and guide the shaking device 2.

[0125] By setting a positioning guide mechanism 73 on the charging position 72 of the base 7 to position and guide the shaking device 2, it is beneficial to accurately place the shaking device 2 on the set position of the charging position 72, so that the charging device can be charged smoothly.

[0126] For example, the positioning guide mechanism 73 includes a first positioning guide rail and a second positioning guide rail disposed on the horizontal support surface of the charging position 72. Both the first positioning guide rail and the second positioning guide rail extend along a first horizontal direction, and are spaced apart along a second horizontal direction. The first horizontal direction is perpendicular to the second horizontal direction. As described above, four first magnets 212 are installed at the bottom of the housing of the shaking device 2. The first magnets 212 protrude downward from the bottom surface of the housing. The first positioning guide rail and the second positioning guide rail cooperate with these four first magnets 212 to divide the four first magnets 212 into two groups distributed along the first horizontal direction. Each group includes two first magnets distributed along the second horizontal direction. The first positioning guide rail and the second positioning guide rail are located between the two first magnets in each group. When placing the shaking device 2, the shaking device 2 is first placed on the horizontal support surface of the charging position 72, and then the shaking device 2 is moved along the first horizontal direction toward the vertical surface of the charging position 72. During the movement, it is guided by the first positioning guide rail and the second positioning guide rail.

[0127] It should be noted that the positioning guide mechanism 73 is not limited to the two positioning guide rails described above. For example, it can also be configured as a positioning guide groove or a positioning guide plate, etc. Such adjustments and changes to the specific structural form of the positioning guide mechanism 73 do not deviate from the principle and scope of the present invention and should be limited to the protection scope of the present invention.

[0128] Those skilled in the art will understand that although some embodiments described herein include certain features included in other embodiments but not others, combinations of features from different embodiments are intended to be within the scope of this application and form different embodiments. For example, any of the claimed embodiments in the claims of this application can be used in any combination.

[0129] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after such changes or substitutions will all fall within the scope of protection of the present invention.

Claims

1. A cell preparation and dispensing system, characterized in that, The device includes a base (7), a dispensing assembly (1) mounted on the base, and multiple shaking devices (2). Cryopreservation bags (5) can be placed on the shaking devices (2). The dispensing assembly (1) is configured to first dispense cell fluid into the cryopreservation bags (5) on the multiple shaking devices (2), and then inject cryopreservation solution into each cryopreservation bag (5) on the shaking device (2). The shaking device (2) is configured to drive the cryopreservation bag (5) to move back and forth when the dispensing assembly (1) injects cryopreservation solution into the cryopreservation bag (5), so that the cell fluid in the cryopreservation bag (5) is mixed with the cryopreservation solution. A charging position (72) is provided on the base (7), and a charging device for charging the shaking device (2) is provided on the charging position (72).

2. The cell preparation and dispensing system according to claim 1, characterized in that, The charging position (71) is also provided with a positioning guide mechanism (73), which is used to position and guide the shaking device (2).

3. The cell preparation and dispensing system according to claim 1, characterized in that, The shaking device (2) and the charging position (71) are fixedly connected by magnetic attraction.

4. The cell preparation and dispensing system according to claim 1, characterized in that, The charging device is either a wireless charging device or a wired charging device.

5. The cell preparation and dispensing system according to claim 1, characterized in that, The cell preparation and dispensing system also includes a cooling component for cooling the cryopreservation bag (5).

6. The cell preparation and dispensing system according to claim 5, characterized in that, The cooling component is a water storage component (3), which can store cold water or ice water. The shaking device (2) is fixedly installed in the water storage component (3), and the cryopreservation bag (5) on the shaking device (2) is located in the cold water or ice water.

7. The cell preparation and dispensing system according to claim 6, characterized in that, The shaking device (2) includes a fixing component (21) and a driving component installed on the fixing component (21). The fixing component (21) is fixedly connected to the water storage component (3). The driving component is provided with a receiving space for accommodating the cryopreservation bag (5). The driving component can drive the cryopreservation bag (5) to move back and forth. The receiving space is provided with a snap-fit ​​structure (225), which snaps into the through hole (51) on the cryopreservation bag (5).

8. The cell preparation and dispensing system according to claim 7, characterized in that, The driving assembly includes a driving mechanism (23) and a moving member (22) connected to the driving mechanism (23). The driving mechanism (23) is capable of driving the moving member (22) to reciprocate. The receiving space is formed in the moving member (22); and / or The containment space is configured to allow the cryopreservation bag (5) to remain in a vertical or tilted position; and / or The water storage component (3) is provided with a fixing seat (31), and the shaking device (2) is fixedly connected to the fixing seat (31) by magnetic attraction; and / or The water storage component (3) is provided with a drain outlet (32) on its bottom wall or side wall.

9. The cell preparation and dispensing system according to claim 8, characterized in that, The movable component (22) includes a vertically arranged body (221), which is connected to the driving mechanism (23). A first limiting structure (222) is provided on the left side of the body (221), a second limiting structure (223) is provided on the right side of the body (221), and a third limiting structure (224) is provided at the bottom of the body (221). The body (221), the first limiting structure (222), the second limiting structure (223), and the third limiting structure (224) together form the receiving space, the top of which is open; and / or The drive mechanism (23) can drive the moving component (22) to move up and down in the vertical direction.

10. The cell preparation and dispensing system according to any one of claims 1 to 9, characterized in that, The cell preparation and dispensing system further includes multiple gas-liquid separation bottles (4), which are respectively installed on corresponding shaking devices (2). The top of each gas-liquid separation bottle (4) is connected to the dispensing assembly (1), and the bottom of each gas-liquid separation bottle (4) is connected to a cryopreservation bag (5) on the shaking device (2); and / or The liquid separation assembly (1) includes a transfer pump (11), a first main pipeline (12), a second main pipeline (13), a first branch pipeline (14), a second branch pipeline (15), a third branch pipeline (16), a first control valve (17), a second control valve (18), and a third control valve (19). The inlet and outlet of the delivery pump (11) are connected to the first main pipeline (12) and the second main pipeline (13) respectively. The first branch pipeline (14) is used to connect the first reservoir component (61) storing cell fluid to the first main pipeline (12). The second branch pipeline (15) is used to connect the second reservoir component (62) storing cryopreservation solution to the first main pipeline (12). There are multiple third branch pipelines (16). The cryopreservation bag (5) on the shaking device (2) is connected to the second main pipeline (13) through the third branch pipelines (16). The first control valve (17) is located on the first branch pipe (14) and is used to control the opening and closing of the first branch pipe (14). The second control valve (18) is located on the second branch pipe (15) and is used to control the opening and closing of the second branch pipe (15). There are multiple third control valves (19). The third control valve (19) is located on the third branch pipe (16) and is used to control the opening and closing of the third branch pipe (16).

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