Water-soluble fertilizer containing amino acid and application thereof

Abstract

The invention provides a water-soluble fertilizer containing amino acid and application of the water-soluble fertilizer. According to the amino acid-containing water-soluble fertilizer disclosed by the invention, lipopeptide is added into the amino acid-containing water-soluble fertilizer, so that efficient nutrient absorption of pasture can be promoted, meanwhile, the soil structure can be improved, the soil microbial activity can be improved, favorable conditions are provided for growth of the pasture, and restoration of degraded grassland is promoted.

Description

TECHNICAL FIELD

[0001] The present application relates to an amino acid-containing water-soluble fertilizer and its application. BACKGROUND

[0002] Grassland plays an important role in preventing wind and sand, fixing carbon and releasing oxygen, and as an important base for livestock production to promote economic development. However, in recent years, due to overgrazing, climate warming and other problems, the grassland ecosystem has been severely degraded, with severe and moderate degradation grasslands accounting for more than 50% of the total area of degraded grasslands. Grassland degradation will lead to a decrease in vegetation coverage and primary productivity, a decrease in climate regulation capacity, a decrease in soil nutrients, a disorder in carbon cycle, a decrease in biodiversity, and frequent natural disasters such as insect and rodent pests, sandstorms, and other natural disasters, which pose a threat to human survival and development. Therefore, it is urgent to repair the degraded grassland.

[0003] Current research shows that timely mowing, regulating grazing, and reasonable application of fertilizers are the main measures for the protection and repair of degraded grassland. There are few fertilizer products on the market that are specifically used for repairing degraded grassland, and the utilization rate of the fertilizer is low. SUMMARY

[0004] The present application aims to develop a new type of fertilizer that is more convenient and has a higher utilization rate, to supplement the nutrients required by the forage grass in a timely manner, to increase the water retention and air permeability of the soil, and to promote the growth of the forage grass in the degraded grassland, thereby promoting the repair of the degraded grassland.

[0005] 1. An amino acid-containing water-soluble fertilizer, comprising a keratin amino acid hydrolysate, a lipopeptide, calcium nitrate, and magnesium nitrate.

[0006] 2. The amino acid-containing water-soluble fertilizer according to item 1, wherein,

[0007] the keratin amino acid hydrolysate is obtained by hydrolysis of human hair with an acidic substance or by enzymatic hydrolysis;

[0008] Preferably, the acidic substance is hydrochloric acid.

[0009] 3. The amino acid-containing water-soluble fertilizer according to item 1, wherein,

[0010] the lipopeptide is surfactin.

[0011] 4. The amino acid-containing water-soluble fertilizer according to item 1, wherein,

[0012] in the amino acid-containing water-soluble fertilizer, the content of the lipopeptide is 5 ppm-10 ppm relative to the content of 100 g / L of free amino acids.

[0013] 5. The amino acid-containing water-soluble fertilizer according to item 1, wherein,

[0014] The free amino acid content in the amino acid-containing water-soluble fertilizer is greater than or equal to 100 g / L, and is further preferably 100-110 g / L.

[0015] 6. The amino acid-containing water-soluble fertilizer according to item 1, wherein,

[0016] The total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate in the amino acid-containing water-soluble fertilizer is greater than or equal to 30 g / L, and is further preferably 30 g / L-35 g / L, relative to the content of 100 g / L of free amino acids.

[0017] 7. Use of the amino acid-containing water-soluble fertilizer according to any one of items 1 to 6 in the preparation of a preparation for restoring the productivity of degraded grassland.

[0018] Preferably, the degraded grassland is degraded steppe.

[0019] Further preferably,

[0020] The free amino acid content in the amino acid-containing water-soluble fertilizer is 0.1 g / L-0.11 g / L, the lipopeptide content is 0.005 ppm-0.01 ppm, and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03 g / L-0.035 g / L when the amino acid-containing water-soluble fertilizer is applied to restore the productivity of degraded grassland.

[0021] 8. Use of the amino acid-containing water-soluble fertilizer according to any one of items 1 to 6 in the preparation of a preparation for promoting the growth and / or yield of forage grass.

[0022] Preferably, the forage grass is selected from one or more than two of Elymus nutans, Avena strigosa, and Medicago sativa.

[0023] 9. Use of the amino acid-containing water-soluble fertilizer according to any one of items 1 to 6 in restoring the productivity of degraded grassland.

[0024] Preferably, the degraded grassland is degraded steppe.

[0025] Further preferably,

[0026] The free amino acid content in the amino acid-containing water-soluble fertilizer is 0.1 g / L-0.11 g / L, the lipopeptide content is 0.005 ppm-0.01 ppm, and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03 g / L-0.035 g / L when the amino acid-containing water-soluble fertilizer is applied to restore the productivity of degraded grassland.

[0027] 10. Use of the amino acid-containing water-soluble fertilizer according to any one of items 1 to 6 in promoting the growth and / or yield of forage grass.

[0028] Preferably, the forage grasses are selected from one or more of the group consisting of: Elymus nutans, Medicago sativa, and Avena strigosa;

[0029] Further preferably,

[0030] The amino acid-containing water-soluble fertilizer has a free amino acid content of 0.01 g / L to 0.11 g / L, a lipopeptide content of 0.005 ppm to 0.01 ppm, and a total calcium and magnesium ion content derived from calcium nitrate and magnesium nitrate of 0.03 g / L to 0.035 g / L when applied to promote the growth and / or yield of forage grasses.

[0031] Advantages

[0032] The present application uses discarded hair as a raw material, which not only disposes of keratin waste, but also increases the amino acid content of the hydrolysis liquid, realizes the resource utilization of waste, and reduces environmental pollution and resource waste.

[0033] The present application provides an amino acid-containing water-soluble fertilizer containing lipopeptides. The addition of lipopeptides to the amino acid-containing water-soluble fertilizer can promote the efficient absorption of nutrients by forage grasses, improve soil structure, increase soil microbial activity, provide favorable conditions for the growth of forage grasses, and promote the repair of degraded grasslands. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 The present application provides an amino acid-containing water-soluble fertilizer containing lipopeptides. The addition of lipopeptides to the amino acid-containing water-soluble fertilizer can promote the efficient absorption of nutrients by forage grasses, improve soil structure, increase soil microbial activity, provide favorable conditions for the growth of forage grasses, and promote the repair of degraded grasslands. DETAILED DESCRIPTION

[0035] The present application will be further described below in conjunction with examples, which should be understood as merely further illustrating and explaining the present application, and not limiting the present application.

[0036] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, illustrative methods and materials are described below. However, if there is a conflict between the definitions in the specification including that of the preceding claimed application and that of the prior art, the specification, including that of the preceding claimed application, is intended to prevail. Furthermore, all patents, patent applications, publications, and descriptions mentioned herein are incorporated by reference in their entirety for the teachings and purposes for which the references are cited.

[0037] In the present application, degraded grassland refers to natural grassland that is affected by adverse natural factors such as drought, wind and sand, water erosion, salinization, waterlogging, changes in groundwater level, or is caused by unreasonable use such as overgrazing and mowing, or is damaged by over-harvesting, over-mowing, and logging, which causes the deterioration of the ecological environment of the grassland, reduces the biological yield of the grassland, and reduces the quality and utilization performance of the grassland, and even loses its value. The specific standards for degraded grassland are referred to in GB 19377-2003.

[0038] In the present application, productivity refers to the ability of a unit area of land to produce biomass or provide ecosystem services over a certain time frame, through natural processes or human management.

[0039] The present application provides an amino acid-containing water-soluble fertilizer, which comprises a keratin amino acid hydrolysate, a lipopeptide, calcium nitrate, and magnesium nitrate.

[0040] In some embodiments of the present application, the amino acid hydrolysate is a keratin amino acid hydrolysate.

[0041] In some embodiments of the present application, the amino acid hydrolysate is obtained by hydrolysis of human hair with an acidic substance or by enzymatic hydrolysis.

[0042] In the present application, the method for preparing the amino acid hydrolysate can use general hydrolysis or enzymatic hydrolysis in the art. The present application does not make any limitation on the hydrolysis or enzymatic hydrolysis, as long as it meets the requirements of the present application.

[0043] In some embodiments of the present application, the acidic substance is hydrochloric acid.

[0044] In a specific embodiment, the lipopeptide is surfactin.

[0045] In the present application, the lipopeptide is purchased from MedChemExpress.

[0046] In some embodiments of the present application, in the amino acid-containing water-soluble fertilizer, the content of the lipopeptide is 5 ppm-10 ppm relative to the content of 100 g / L of free amino acid; for example, the content of the lipopeptide can be 5 ppm, 6 ppm, 7 ppm, 8 ppm, 9 ppm, 10 ppm, or any range therebetween, relative to the content of 100 g / L of free amino acid.

[0047] In some embodiments of the present application, in the amino acid-containing water-soluble fertilizer, the content of free amino acid is greater than or equal to 100 g / L, for example, the content of free amino acid is 100 g / L, 150 g / L, 200 g / L, 250 g / L, 300 g / L, 350 g / L, 400 g / L, 450 g / L, 500 g / L, 550 g / L, 600 g / L, 650 g / L, 700 g / L, 750 g / L, 800 g / L, 850 g / L, 900 g / L, 950 g / L, 1000 g / L, or any range therebetween.

[0048] The term "free amino acid" refers to an amino acid that is not part of a peptide or polypeptide (e.g., is not linked to another amino acid via a peptide bond). A free amino acid is free in solution (rather than linked to at least one other amino acid via, e.g., a dipeptide bond), but can be associated with a salt or other component in solution.

[0049] In some embodiments of the present application, the content of free amino acid in the amino acid-containing water-soluble fertilizer is 100-110 g / L.

[0050] In some embodiments of the present application, the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate in the amino acid-containing water-soluble fertilizer is greater than or equal to 30 g / L, relative to the content of 100 g / L of free amino acid, and is further preferably 30 g / L-35 g / L. For example, the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 30 g / L, 40 g / L, 50 g / L, 60 g / L, 70 g / L, 80 g / L, 90 g / L, 100 g / L, 110 g / L, 120 g / L, 130 g / L, 140 g / L, 150 g / L, 160 g / L, 170 g / L, 180 g / L, 190 g / L, 200 g / L, or any range therebetween, relative to the content of 100 g / L of free amino acid.

[0051] In some embodiments of the present application, the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate in the amino acid-containing water-soluble fertilizer is 30 g / L-35 g / L, relative to the content of 100 g / L of free amino acid; for example, the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate can be 30 g / L, 31 g / L, 32 g / L, 33 g / L, 34 g / L, 35 g / L, or any range therebetween.

[0052] The present application provides the use of the above-mentioned amino acid-containing water-soluble fertilizer in the preparation of a preparation for restoring the productivity of degraded grassland.

[0053] In some embodiments of the present application, the degraded grassland is a slightly degraded grassland, and the specific standard is referred to GB 19377-2003.

[0054] In some embodiments of the present application, the content of free amino acid in the amino acid-containing water-soluble fertilizer is 0.1 g / L-0.11 g / L when the amino acid-containing water-soluble fertilizer is applied to restore degraded grassland, and the content of lipopeptide is

[0055] 0.005ppm-0.01ppm, the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03g / L-0.035g / L; for example, the free amino acid content can be 0.1g / L, 0.101g / L, 0.102g / L, 0.103g / L, 0.104g / L, 0.105g / L, 0.106g / L, 0.107g / L, 0.108g / L, 0.109g / L, 0.11g / L or any range therebetween; the lipopeptide content can be 0.005ppm, 0.006ppm, 0.007ppm, 0.008ppm, 0.009ppm, 0.01ppm or any range therebetween; the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate can be 0.03g / L, 0.031g / L, 0.032g / L, 0.033g / L, 0.034g / L, 0.035g / L or any range therebetween.

[0056] The application provides application of the above-mentioned amino acid-containing water-soluble fertilizer in preparation of a preparation for promoting growth and / or yield of forage grass.

[0057] In some embodiments of the application, the forage grass is selected from one or more of Elymus nutans, Avena strigosa and Medicago sativa.

[0058] The application provides application of the above-mentioned amino acid-containing water-soluble fertilizer in restoring productivity of degraded grassland.

[0059] In some embodiments of the application, when the amino acid-containing water-soluble fertilizer is applied to restore productivity of degraded grassland, the free amino acid content is 0.1g / L-0.11g / L, the lipopeptide content is 0.005ppm-0.01ppm, and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03g / L-0.035g / L. For example, the free amino acid content can be 0.1g / L, 0.101g / L, 0.102g / L, 0.103g / L, 0.104g / L, 0.105g / L, 0.106g / L, 0.107g / L, 0.108g / L, 0.109g / L, 0.11g / L or any range therebetween; the lipopeptide content can be 0.005ppm, 0.006ppm, 0.007ppm, 0.008ppm, 0.009ppm, 0.01ppm or any range therebetween; and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate can be 0.03g / L, 0.031g / L, 0.032g / L, 0.033g / L, 0.034g / L, 0.035g / L or any range therebetween.

[0060] Preparation Example 1: Preparation of amino acid hydrolysate of keratin:

[0061] (1) Select clean, no impurities, no mildew hair 500g, rinse with clean water, remove the surface dust, dirt and grease and other impurities, dry or dry with a pulverizer to 40-80 mesh, to increase the contact area with hydrochloric acid, improve the hydrolysis efficiency;

[0062] (2) Hydrolysis reaction: using 6mol / L hydrochloric acid solution, according to the solid-liquid ratio 1:3, 500g hair powder and 1500mL hydrochloric acid solution into the reaction vessel, heated under reflux stirring at 110℃ for 8-12h, until the hydrolysis is complete.

[0063] Hydrolysis process regularly sampling detection, to determine the end point of hydrolysis, the specific steps are: take 5mL hydrolysis solution, add 10% NaOH solution, a small amount of distilled water and activated carbon, water bath heating 10min, drop 2% CuSO4 solution 3-4 drops, if the solution is no blue, indicating that the hydrolysis is complete.

[0064] (3) Neutralization treatment: after the hydrolysis is completed, using ammonia to adjust the pH to 5.5-6.5, get the amino acid hydrolysate of keratin.

[0065] Preparation example 2: other protein by enzymolysis of amino acid aqueous solution

[0066] The corn soaking water of monosodium glutamate factory waste is collected and filtered for use;

[0067] The corn skin of monosodium glutamate factory, corn residue of starch factory, and bagasse of sugar factory are mixed to obtain a raw material mixture, which is mixed and soaked for 2-3h at a solid-liquid ratio of 1:10 of the raw material mixture and corn soaking water, 2% of yeast is added, and the fermentation is carried out at 30℃ for 10h, and then inactivated, and 1.5% of protease is added for enzymolysis at 50℃ for 40min, and then filtered to obtain filtrate and residue, and the filtrate is the amino acid aqueous solution of other protein, which is ready for use.

[0068] Preparation example 3: other protein by hydrolysis of amino acid aqueous solution

[0069] The defatted soybean meal is mixed with warm water at a solid-liquid ratio of 1:10, and then 6mol / L hydrochloric acid solution is added to adjust the pH value to 1-2, and the reaction is carried out at 100-120℃ for 6-24h, and then the pH value is adjusted to 5-6 by using NaOH solution after the hydrolysis is completed, and the residue is removed by filtration, and the filtrate is the amino acid aqueous solution of soybean protein, which is ready for use.

[0070] Example

[0071] Example 1 preparation of amino acid containing water soluble fertilizer added with lipopeptide

[0072] (1) To the amino acid hydrolysate of keratin in Preparation Example 1, add 20 g of calcium nitrate tetrahydrate and 25.3 g of magnesium nitrate hexahydrate, and continuously stir during addition to ensure complete dissolution and mixing; the total content of calcium and magnesium ions in the final product is 35 g / L.

[0073] (2) Slowly add surfactin (purchased from MedChemExpress) to the reaction system, and use a high-speed blender to stir to ensure uniform dispersion of the surfactin in the solution.

[0074] (3) Filtration and separation: filter the above solution to separate insoluble impurities and precipitates, obtaining a clear crude amino acid solution.

[0075] (4) Concentration: use vacuum concentration method; after concentration, the amino acid-containing water-soluble fertilizer is placed in a clean container and stored in a low-temperature, dry, cool place.

[0076] Among them, the content of surfactin in the final product is 8 ppm, and the content of amino acid is 100 g / L.

[0077] The difference between Example 2-10 and Comparative Examples 1-3 is only in Table 1, and the rest is the same as Example 1.

[0078] Table 1

[0079]

[0080]

[0081] Note: The amino acid-containing water-soluble fertilizer in Comparative Example 3 is purchased from Syngenta (Fertilizer (2023) No. 18947).

[0082] The amino acid in Example 10 is purchased from Huto's 80 Amino Acid Powder.

[0083] Experimental Example

[0084] Experimental Example 1: Indoor potting test of amino acid-containing water-soluble fertilizer

[0085] 1. Plant material: Collect mature, full and disease-free Elymus nutans, Avena sativa and Medicago sativa seeds from Chifeng, Inner Mongolia. After soaking the collected seeds in 0.5% potassium permanganate solution for 15 minutes, rinse them with distilled water and set aside.

[0086] 2. Germination rate determination: Take 18 culture dishes (15 cm x 15 cm x 3 cm) containing filter paper, and use the amino acid-containing water-soluble fertilizer in Example 1 to set up 6 treatment groups, with 3 repeats in each group. Place 100 Elymus nutans seeds, Medicago sativa seeds and Avena sativa seeds in each of the 3 groups, respectively. The 6 treatments are as follows and are denoted as:

[0087] CK (control group) 20 mL of water;

[0088] T1: 20 mL of amino acid-containing water-soluble fertilizer diluted 1500 times;

[0089] T2: 20 mL of amino acid-containing water-soluble fertilizer diluted 1000 times;

[0090] T3: 20 mL of amino acid-containing water-soluble fertilizer diluted 500 times;

[0091] T4: 20 mL of amino acid-containing water-soluble fertilizer diluted 250 times;

[0092] T5: 20 mL of amino acid-containing water-soluble fertilizer diluted 200 times.

[0093] After 7 days, the seed germination rate was calculated (with the standard of 5 mm of the embryo bud breaking through the seed coat), as shown in Table 2.

[0094] Table 2

[0095]

[0096] Note: Different lowercase letters indicate significant differences (P < 0.05) between different treatments.

[0097] As can be seen from the results in Table 2, the seed germination rates of the T1 and T2 treatment groups were significantly higher than that of the control group, and the T2 treatment group had the best effect. The seed germination rates of the T2 treatment group were increased by 5.5%, 4.3%, and 3.6% compared with the control group, respectively. With the increase of the concentration of the amino acid-containing water-soluble fertilizer, the seed germination rate was inhibited and lower than that of the control group.

[0098] Example 2: Indoor pot experiment of amino acid-containing water-soluble fertilizer

[0099] Indoor pot system: peat soil was used as the cultivation substrate, and 39 planting pots were taken. Each planting pot (upper diameter 18.5 cm, bottom diameter 13 cm, height 15 cm) was filled with 600 g of substrate soil, and the substrate was subjected to high-temperature sterilization treatment before planting to kill bacteria and insect eggs.

[0100] The 52 planting pots were divided into 14 groups, 3 pots in each group, and 20 seeds of each of the three kinds of grasses were evenly sown in each group, and a layer of thin substrate soil was covered. The seeds of the three kinds of grasses were the same as in Experimental Example 1. Each group was respectively irrigated and applied with the 1000-fold diluted water-soluble fertilizer containing amino acids in the control group (denoted as CK, water was applied), Examples 1-10 and Comparative Examples 1-3, and the culture dishes and the sowed planting pots were placed in an artificial climate incubator for cultivation, with the temperature being 20°C, the humidity being 60%, and the light time being 16h / d. The application was performed once every other week, for a total of 2 times, and the agronomic traits were measured one week after the end of the second application.

[0101] Table 3

[0102]

[0103] Note: Different lowercase letters indicate significant differences (P<0.05) between different treatments

[0104] As can be seen from the results in Table 3, the germination rate, plant height and aboveground biomass of the pasture after application of Examples 1-7 were all improved to different degrees.

[0105] The germination rate results show that the germination rates of the three kinds of grasses after application of Examples 1-3 are relatively high and are significantly higher than those of the control group and Comparative Examples 1-2 and Examples 8-10, and Example 1 has the best overall treatment effect. Compared with the control group, the germination rate of the rostrate agropyron in Example 1 is increased by 13.3%, the germination rate of the alfalfa is increased by 12.1%, and the germination rate of the oat grass is increased by 12.9%.

[0106] The plant height results show that the plant heights of the three kinds of grasses after application of Examples 1-3 are relatively high and are significantly higher than those of the control group and Comparative Examples 1-2 and Examples 8-10, and Example 1 has the best overall treatment effect. Compared with the control group, the plant height of the rostrate agropyron in Example 1 is increased by 15.2%, the plant height of the alfalfa is increased by 15.0%, and the plant height of the oat grass is increased by 13.4%.

[0107] The aboveground biomass results show that the aboveground biomass of the three kinds of grasses after application of Examples 1-3 is relatively high and is significantly higher than that of the control group and Comparative Examples 1-2 and Examples 8-10, and Example 1 has the best overall treatment effect. Compared with the control group, the aboveground biomass of the rostrate agropyron in Example 1 is increased by 19.4%, the aboveground biomass of the alfalfa is increased by 20.3%, and the aboveground biomass of the oat grass is increased by 14.6%.

[0108] Experimental Example 3 Field Application Effect of Degraded Grassland

[0109] A light degradation grassland test field with relatively consistent conditions was selected in Chifeng City, Inner Mongolia. The light degradation grassland test field was selected according to the light degradation grassland standard in GB 19377-2003. Elymus nutans, Medicago sativa and Avena strigosa were selected as test crops. The test treatment was set as follows: the light degradation grassland test field was divided into 14 groups of 1 m x 1 m plots, and each group had 3 plots. Each of the 3 plots in each group was evenly sown with 200 seeds of Elymus nutans, Medicago sativa and Avena strigosa seeds, respectively. The seeds of Elymus nutans, Medicago sativa and Avena strigosa were the same as in Experimental Example 1. Each group was irrigated and root-applied with the control group (denoted as CK, applying water), the diluted 1000 times amino acid-containing water-soluble fertilizer in Examples 1-10 and Comparative Examples 1-3, respectively. The application was performed once every other week, and a total of 2 times. One week after the end of the second application, the plant height, vegetation coverage and aboveground biomass of each treatment sample were measured (as shown in Table 4).

[0110] Table 4: Field effect of different treatments on forage grass

[0111]

[0112]

[0113] Note: Different lowercase letters indicate significant differences (P < 0.05) between different treatments

[0114] As can be seen from the results in Table 3, the germination rate, plant height, vegetation coverage and aboveground biomass of the forage grass after applying Examples 1-10 were improved to varying degrees.

[0115] The germination rate results show that the germination rate of Elymus nutans, Medicago sativa and Avena strigosa after applying Examples 1-3 is the highest, among which the overall treatment effect of Example 1 is the best. Compared with the control group, the germination rate of Elymus nutans in Example 1 is increased by 9.8%, the germination rate of Medicago sativa is increased by 10.3%, and the germination rate of Avena strigosa is increased by 9.3%, which are significantly higher than those of the control group and Comparative Examples 1-2 and Examples 8-10.

[0116] The plant height results show that the plant height of Elymus nutans, Medicago sativa and Avena strigosa after applying Examples 1-3 is the highest, among which the overall treatment effect of Example 1 is the best. Compared with the control group, the plant height of Elymus nutans in Example 1 is increased by 22.4%, the plant height of Medicago sativa is increased by 17.5%, and the plant height of Avena strigosa is increased by 19.9%.

[0117] The vegetation coverage results show that the vegetation coverage of Elymus nutans, Medicago sativa and Avena strigosa after applying Examples 1-3 is the highest, among which the overall treatment effect of Example 1 is the best. Compared with the control group, the vegetation coverage of Elymus nutans in Example 1 is increased by 24.1%, the vegetation coverage of Medicago sativa is increased by 18.6%, and the vegetation coverage of Avena strigosa is increased by 19.9%.

[0118] The results of aboveground biomass show that the aboveground biomass of P. distans, M. sativa and A. sativa is the highest after applying Examples 1-3, and the overall treatment effect of Example 1 is the best. Compared with the control group, the aboveground biomass of P. distans in Example 1 is increased by 16.3%, the aboveground biomass of M. sativa is increased by 11.4%, and the aboveground biomass of A. sativa is increased by 16.5%.

[0119] Among them, in the indoor pot experiment, the biomass increase effect of Example 1 and Comparative Example 1 is not much different, but in the degraded grassland experiment, the biomass increase effect of Example 1 is much higher than that of Comparative Example 1.

[0120] Although the present case has been disclosed as above with examples, it is not intended to limit the present case, and anyone with ordinary knowledge in the art can make some changes and modifications without departing from the spirit and scope of the present case, so the protection scope of the present case shall be subject to the scope defined in the following patent application.

Claims

1. A water-soluble fertilizer containing amino acids, comprising keratin amino acid hydrolysate, lipopeptides, calcium nitrate, and magnesium nitrate.

2. The water-soluble fertilizer containing amino acids according to claim 1, wherein, The keratin amino acid hydrolysate is obtained by hydrolyzing human hair with acidic substances or by enzymatic hydrolysis; Preferably, the acidic substance is hydrochloric acid.

3. The water-soluble fertilizer containing amino acids according to claim 1, wherein, The lipopeptide is a surfactant.

4. The water-soluble fertilizer containing amino acids according to claim 1, wherein, In the water-soluble fertilizer containing amino acids, the lipopeptide content is 5ppm-10ppm relative to the free amino acid content of 100g / L.

5. The water-soluble fertilizer containing amino acids according to claim 1, wherein, The water-soluble fertilizer containing amino acids has a free amino acid content of 100 g / L or greater, more preferably 100-110 g / L.

6. The water-soluble fertilizer containing amino acids according to claim 1, wherein, In the amino acid-containing water-soluble fertilizer, relative to the free amino acid content of 100 g / L, the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is greater than or equal to 30 g / L, and more preferably 30 g / L-35 g / L.

7. The use of the amino acid-containing water-soluble fertilizer according to any one of claims 1 to 6 in the preparation of a formulation for restoring the productivity of degraded grassland; Preferably, the degraded grassland is a degraded steppe; More preferably, When the water-soluble fertilizer containing amino acids is applied to restore degraded grassland, the free amino acid content is 0.1 g / L to 0.11 g / L, the lipopeptide content is 0.005 ppm to 0.01 ppm, and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03 g / L to 0.035 g / L.

8. The use of the amino acid-containing water-soluble fertilizer according to any one of claims 1 to 6 in the preparation of formulations that promote the growth and / or yield of forage grasses; Preferably, the forage grass is selected from one or more of the following: crested wheatgrass, oat grass, and alfalfa.

9. The application of the amino acid-containing water-soluble fertilizer according to any one of claims 1 to 6 in restoring the productivity of degraded grassland; Preferably, the degraded grassland is a degraded steppe; More preferably, When the water-soluble fertilizer containing amino acids is applied to restore the productivity of degraded grassland, the free amino acid content is 0.1 g / L to 0.11 g / L, the lipopeptide content is 0.005 ppm to 0.01 ppm, and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03 g / L to 0.035 g / L.

10. The use of the amino acid-containing water-soluble fertilizer according to any one of claims 1 to 6 in promoting the growth and / or yield of forage grass; Preferably, the forage grass is selected from one or more of the following: Leymus chinensis, alfalfa, and oat grass; More preferably, When the water-soluble fertilizer containing amino acids is applied to promote the growth and / or yield of forage grass, the free amino acid content is 0.01 g / L to 0.11 g / L, the lipopeptide content is 0.005 ppm to 0.01 ppm, and the total content of calcium and magnesium ions derived from calcium nitrate and magnesium nitrate is 0.03 g / L to 0.035 g / L.

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