Siphon micro-channel type visible component counting plate and use method

Abstract

The invention relates to the technical field of animal cell morphology inspection, in particular to a siphon micro-channel type visible component counting plate and a using method thereof.The siphon micro-channel type visible component counting plate comprises an upper cover plate and a lower bottom plate, a first detection cavity and a second detection cavity are formed between the upper cover plate and the lower bottom plate, and the first detection cavity and the second detection cavity are formed in the counting plate; the thickness of the second detection cavity is greater than that of the first detection cavity, so that a thin detection area and a thick detection area are formed, detection of visible components with different sizes in a sample is facilitated, the sample detection is quicker, a quartz counting chamber used by a traditional visible component analyzer is replaced by the disposable counting plate, and the detection efficiency is improved. The device has the advantages that the maintenance cost and the single detection cost of the instrument are reduced, visible components of a sample are detected in the first detection cavity and the second detection cavity of the counting plate, the requirement on the detected sample quantity is very low, and the problems that body fluids such as animal blood and urine are difficult to collect and the sample quantity is small are solved.

Description

Technical Field

[0001] This invention relates to the field of animal cell morphology testing technology, and in particular to a siphon microfluidic formed element counting plate and its usage method. Background Technology

[0002] Laboratory testing of cell morphology and formed elements in animal body fluid specimens such as blood, feces, and urine using conventional methods is a complex and time-consuming task, requiring multiple steps including dilution, centrifugation, staining, slide preparation, and drying. Specimen preparation is not only time-consuming but also demands a high level of operator skill, making it susceptible to significant human error.

[0003] Therefore, it is necessary to design a structurally sound formed element counting plate to reduce the workload of sample centrifugation, slide pushing, drying, etc., while avoiding inaccurate detection due to subjective factors such as operator experience and skill level. Summary of the Invention

[0004] The purpose of this invention is to provide a siphon microfluidic formed element counting plate and its usage method, which solves the technical problems of existing laboratory methods for examining cell morphology or formed elements in animal blood, feces, urine and other body fluid specimens. These problems include the long preparation time, high technical requirements for operators, and susceptibility to human factors.

[0005] To achieve the above objectives, the present invention provides a siphon microchannel shaped element counting plate, which includes an upper cover plate and a lower base plate. The upper cover plate and the lower base plate are bonded together by double-sided adhesive and are located on the upper surface of the lower base plate. A first detection chamber and a second detection chamber are disposed between the upper cover plate and the lower base plate. A sample inlet is disposed at the center of the upper cover plate. The first detection chamber and the second detection chamber are respectively located on both sides of the sample inlet. A first overflow outlet is disposed at the end of the first detection chamber away from the sample inlet, and a second overflow outlet is disposed at the end of the second detection chamber away from the sample inlet. Both the first detection chamber and the second detection chamber have a gradually changing elliptical structure. The guide edges of the first detection chamber and the second detection chamber both change from narrow to wide along the gradually changing elliptical structure. The thickness of the second detection chamber is greater than the thickness of the first detection chamber. The length of the first detection chamber is less than the length of the second detection chamber. The width of the first detection chamber is greater than the width of the second detection chamber.

[0006] The upper cover plate and the lower base plate are provided with limit notches on both sides. The surface of the upper cover plate is provided with a marking arrow. The marking arrow is located at the end of the first detection cavity away from the sample application port. The upper cover plate and the lower base plate are provided with arc-shaped guide edges on both sides near the end of the marking arrow.

[0007] The sample dispensing port is provided with a spacer block in the middle, which divides the sample dispensing port into a first sample dispensing area and a second sample dispensing area. The first sample dispensing area is connected to the first detection cavity, and the second sample dispensing area is connected to the second detection cavity.

[0008] The height of the spacer block is lower than the upper surface of the sample dispensing port.

[0009] The opening size of the first overflow port is smaller than the opening size of the second overflow port.

[0010] The upper surface of the upper cover plate is provided with a first recess and a second recess. The first recess is located in the middle of the flow channel of the first detection cavity and forms a first observation window in the first detection cavity. The second recess is located in the middle of the flow channel of the second detection cavity and forms a second observation window in the second detection cavity.

[0011] The depth of the first depression is greater than the depth of the second depression.

[0012] The present invention also provides a method for using a siphon microchannel type formed element counting plate, applied to the siphon microchannel type formed element counting plate as described above, comprising the following steps:

[0013] After collecting the samples, add them to a container with the stained solution, mix thoroughly, and then use a pipette to take a certain amount of the stained mixed specimen.

[0014] Place the counting chamber horizontally on the table, use a pipette to add the stained mixed specimen into the sample inlet, and let it stand for a period of time;

[0015] Following the direction of the arrow above the counting plate, align the counting plate with the placement slot in the instrument compartment and push the counting plate into the placement slot until the limiting notches on both sides of the counting plate are locked by the corresponding limiting posts. Close the compartment and the instrument will begin testing.

[0016] Before the test, the instrument automatically determines the liquid filling status, selects observation positions at preset positions in the first and second observation windows respectively, and divides them into 6 square detection areas;

[0017] Divide the six small squares of the first observation window into b equal parts along the X direction and a equal parts along the Y direction. Each small square generates a*b detection fields of view, and the entire first observation window generates 6*a*b detection fields of view.

[0018] Divide the six small squares of the second observation window into B equal parts along the X direction and A equal parts along the Y direction. Each small square generates A*B detection fields of view, and the entire second observation window generates 6*A*B detection fields of view.

[0019] After the counting plate is loaded in the instrument, the system automatically positions itself at the origin and scans and detects each subdivided area of ​​the first observation window and the second observation window along the X and Y axes in the order of 1-2-3-4-5-6.

[0020] The test is complete. The instrument door is opened, the counting plate is removed, and the test is finished.

[0021] This invention discloses a siphon microfluidic formed element counting plate and its usage method, comprising an upper cover plate and a lower base plate. A first detection chamber and a second detection chamber are disposed between the upper cover plate and the lower base plate. By setting the first detection chamber and the second detection chamber in the counting plate, and the thickness of the second detection chamber being greater than the thickness of the first detection chamber, two detection areas of thin and thick are formed, which facilitates the detection of formed elements of different sizes in the sample. This solves the problem of needing multiple focusing at the same position in a single-thickness detection channel, making sample detection faster. At the same time, by using a disposable counting plate, the quartz counting cell used in traditional formed element analyzers is replaced, eliminating the complex liquid path structure and the consumption of various detection reagents. This not only reduces the maintenance cost and the cost per detection, but also solves the problems of simplifying and miniaturizing the detection instrument. Furthermore, the formed elements of the sample are detected in the first detection chamber and the second detection chamber of the counting plate, requiring a very small sample volume, solving the problems of difficult collection and small sample volume of animal blood, urine, and other bodily fluids. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a schematic diagram of the structure of the siphon microchannel formed element counting plate provided by the present invention.

[0024] Figure 2 This is a schematic diagram of the cross-sectional structure of the siphon microchannel formed element counting plate provided by the present invention at the sample inlet.

[0025] Figure 3 This is a schematic diagram of sample sampling in the method of using the siphon microchannel formed element counting plate provided by the present invention.

[0026] Figure 4 This is a schematic diagram of sample addition in the method of using the siphon microchannel formed element counting plate provided by the present invention.

[0027] Figure 5 This is a schematic diagram of the loading of the counting plate in the method of using the siphon microchannel shaped element counting plate provided by the present invention.

[0028] Figure 6 This is a schematic diagram of the testing process in the method of using the siphon microchannel formed element counting plate provided by the present invention.

[0029] Figure 7 This is a flowchart illustrating the steps of using the siphon microchannel morphological counting plate provided by the present invention.

[0030] 101-Upper cover plate, 102-Lower base plate, 103-First detection chamber, 104-Second detection chamber, 105-Sample dispensing port, 106-First overflow port, 107-Second overflow port, 108-Limiting notch, 109-Marking arrow, 110-Arc-shaped guide edge, 111-Spacer block, 112-First sample dispensing area, 113-Second sample dispensing area, 114-First observation window, 115-Second observation window. Detailed Implementation

[0031] Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0032] Please see Figure 1 and Figure 2This invention provides a siphon microchannel type formed element counting plate, which includes an upper cover plate 101 and a lower base plate 102. The upper cover plate 101 and the lower base plate 102 are bonded together by double-sided adhesive and are located on the upper surface of the lower base plate 102. A first detection cavity 103 and a second detection cavity 104 are disposed between the upper cover plate 101 and the lower base plate 102. A sample dispensing port 105 is disposed at the center of the upper cover plate 101. The first detection cavity 103 and the second detection cavity 104 are respectively located on both sides of the sample dispensing port 105, with the first detection cavity 103 being farther away from the sample dispensing port 105. One end of the 5 is provided with a first overflow port 106, and the end of the second detection cavity 104 away from the sample inlet 105 is provided with a second overflow port 107. Both the first detection cavity 103 and the second detection cavity 104 have a gradually changing elliptical structure. The guide edges of the first detection cavity 103 and the second detection cavity 104 are both narrow and wide along the gradually changing elliptical structure. The thickness of the second detection cavity 104 is greater than the thickness of the first detection cavity 103. The length of the first detection cavity 103 is less than the length of the second detection cavity 104. The width of the first detection cavity 103 is greater than the width of the second detection cavity 104.

[0033] In this embodiment, by setting the first detection chamber 103 and the second detection chamber 104 in the counting plate, and the thickness of the second detection chamber 104 being greater than the thickness of the first detection chamber 103, two detection areas of thin and thick are formed. This facilitates the detection of formed elements of different sizes in the specimen, solves the problem of needing multiple focusing at the same position in a single thickness detection channel, and makes sample detection faster. At the same time, by using a disposable counting plate to replace the quartz counting cell used in traditional formed element analyzers, the complex liquid path structure is eliminated, and the consumption of various detection reagents is eliminated. This not only reduces the maintenance cost and the cost per detection, but also solves the problems of simplifying and miniaturizing the detection instrument. Furthermore, the formed elements of the sample are detected in the first detection chamber 103 and the second detection chamber 104 of the counting plate, and the amount of sample to be detected is very small, solving the problem of difficult collection and small sample volume of animal blood, urine and other body fluids.

[0034] Furthermore, the segmented layout of thick and thin dual channels solves the problem that when liquid flows through uneven channels in a single thick and thin layout, local impact and backflow can cause air bubbles, resulting in the detection chamber not being fully filled and affecting the detection.

[0035] The thick and thin dual-channel segmented layout allows the liquid to flow into the thick and thin testing areas independently without interference. This solves the problem that when liquid flows from the thick area to the thin area in a single thick-thin layout, the concentration of formed elements in the thin area decreases due to the viscosity of the liquid, which increases the risk of false negatives for testing items in the thin area.

[0036] The liquid encapsulates the cells, and under the siphon effect, the cells are spread evenly in the thick and thin channels of the counting plate, which solves the problems of inconsistent sample membrane thickness, uneven cell distribution, overlap, and rupture caused by human factors when pushing traditional specimens.

[0037] Furthermore, the lower base plate 102 is made of rigid glass or plastic with small deformation and good light transmittance. After the formed components settle onto the surface of the lower base plate 102, due to the small deformation of the lower base plate 102, all components are at the same reference. This prevents the need for multiple focusing attempts when measuring components of similar size due to the magnification effect. The upper cover plate 101 is made of plastic with good light transmittance and high plasticity. The upper cover plate 101 is provided with the sample application hole. After being bonded to the lower base plate 102, it is corrected under the rigid action of the lower base plate 102, making the upper cover plate 101 parallel to the lower base plate 102. The microchannel cavity has a uniform thickness and a consistent light refraction angle, ensuring the imaging quality of each area during detection.

[0038] The upper cover plate 101 and the lower base plate 102 are bonded together using 80-120 micrometer thick PVC double-sided adhesive, forming two microfluidic cavities of uniform thickness, namely the first detection cavity 103 and the second detection cavity 104. The connection method between the lower base plate 102 and the upper cover plate 101 includes, but is not limited to, double-sided adhesive bonding, hot melt welding, laser welding, ultrasonic welding, etc.

[0039] Furthermore, the microchannel structure in the upper cover plate 101 can also be implemented in the lower base plate 102. That is, the upper cover plate 101 is a rigid flat plate with good light transmittance, and the lower base plate 102 is bonded to the upper cover plate 101 after the flow channel features are made.

[0040] In this embodiment, the microchannels of the first detection chamber 103 and the second detection chamber 104 are respectively placed on both sides of the sample inlet 105. The first detection chamber 103 and the second detection chamber 104 are composed of two gradient ellipses of different sizes. The guide edge gradually widens along the gradient ellipses and finally converges at the first overflow port 106 and the second overflow port 107, respectively, so that the liquid flows smoothly and prevents the liquid from failing to fill the chamber effectively due to abrupt changes in channel width, resulting in gaps or air bubbles that affect imaging observation. The first overflow port 106 and the second overflow port 107 are both placed at the end of the corresponding flow channel, with a crescent-shaped structure, parallel to the end guide edge, which helps the chamber to remove air bubbles and fill with liquid. The flow channel of the second detection chamber 104 is relatively high and narrow, while the flow channel of the first detection chamber 103 is relatively low and wide. The thick flow channel is used to detect larger formed elements in the sample, and the thin flow channel is used to detect smaller formed elements in the sample.

[0041] The thick and thin partition design of the first detection chamber 103 and the second detection chamber 104 meets the requirements for counting the formed elements of body fluid samples such as animal blood, feces, urine, pleural effusion, peritoneal fluid, and cerebrospinal fluid. The universal design avoids the need to use different counting plates for different body fluid samples. The height of the thin chamber cross section is within 50-150 micrometers, and the height of the thick chamber cross section is within 100-500 micrometers. The preferred height of the thin chamber cross section in this technical solution is 100 micrometers, and the preferred height of the thick chamber cross section is 350 micrometers.

[0042] Furthermore, both sides of the upper cover plate 101 and the lower base plate 102 are provided with limiting notches 108. The surface of the upper cover plate 101 is provided with a marking arrow 109, which is located at the end of the first detection cavity 103 away from the sample application port 105. Both sides of the upper cover plate 101 and the lower base plate 102 near the end of the marking arrow 109 are provided with arc-shaped guide edges 110.

[0043] Furthermore, a spacer block 111 is provided in the middle of the sample dispensing port 105. The spacer block 111 divides the sample dispensing port 105 into a first sample dispensing area 112 and a second sample dispensing area 113. The first sample dispensing area 112 is connected to the first detection cavity 103, and the second sample dispensing area 113 is connected to the second detection cavity 104. The height of the spacer block 111 is lower than the upper surface of the sample dispensing port 105, and the spacer block 111 can be omitted when the siphon forces of the first detection cavity 103 and the second detection cavity 104 are similar.

[0044] In this embodiment, a spacer block 111 is provided in the middle of the sample dispensing port 105. The height of the spacer block 111 is lower than the upper surface of the sample dispensing port 105. When dispensing the sample, the sample drips onto the spacer block 111 and overflows into the first sample dispensing area 112 and the second sample dispensing area 113 on both sides (both the first sample dispensing area 112 and the second sample dispensing area 113 are semi-circular hole structures). This avoids two sample dispensings and prevents the liquid in the flow channel from not being fully filled due to the different siphon forces of the two flow channels, thus avoiding the generation of air pockets or voids.

[0045] Furthermore, the opening size of the first overflow port 106 is smaller than the opening size of the second overflow port 107, so that the siphon force obtained by the flow channel of the first detection cavity 103 (thin area) is smaller than that of the flow channel of the second detection cavity 104 (thick area), and both the first overflow port 106 and the second overflow port 107 can be designed as crescent-shaped, square, round, waist-shaped or other structures.

[0046] Furthermore, the upper surface of the upper cover plate 101 is provided with a first recess and a second recess. The first recess is located in the middle of the flow channel of the first detection cavity 103 and forms a first observation window 114 in the first detection cavity 103. The second recess is located in the middle of the flow channel of the second detection cavity 104 and forms a second observation window 115 in the second detection cavity 104.

[0047] In this embodiment, the flatness of the flow channel of the upper cover plate 101 is not less than 50 micrometers, and its parallelism with the lower base plate 102 is not less than 50 micrometers; the upper cover plate 101 is provided with two recessed observation areas, namely the first observation window 114 (thin area observation window) and the second observation window 115 (thick area observation window), with a light transmittance ≥90%; a symmetrical "shuttle-shaped" sample dispensing port 105 is provided between the two observation windows, and a flow channel interval is provided between the sample dispensing ports 105. The blocking block 111 divides the sample dispensing port 105 into the first sample dispensing area 112 and the second sample dispensing area 113 (both the first sample dispensing area 112 and the second sample dispensing area 113 are semi-circular structures); a crescent-shaped overflow port is provided at the end of each of the two observation areas to obtain siphon force so that the liquid in the sample dispensing port 105 can spread evenly in the flow channel; because the flow channel volume of the thick area is larger, the overflow opening of the thick area is larger than that of the thin area, so that the liquid can be easily sucked into the flow channel and spread evenly.

[0048] Please see Figures 3 to 7 The present invention also provides a method for using a siphon microchannel type formed element counting plate, applied to the siphon microchannel type formed element counting plate as described above, comprising the following steps:

[0049] S1: After collecting the sample, add it to the sample container with the staining solution, mix thoroughly, and then use a pipette to take a certain amount of the stained mixed specimen.

[0050] S2: Place the counting chamber horizontally on the table, use a pipette to add the stained mixed specimen into the sample application port 105, and let it stand for a period of time;

[0051] S3: According to the direction of the mark arrow 109 above the counting plate, align the counting plate with the placement slot in the instrument compartment and push the counting plate into the placement slot until the limiting notches 108 on both sides of the counting plate are locked by the corresponding limiting posts. Close the compartment and the instrument will start testing.

[0052] S4: Before the test, the instrument automatically determines the liquid filling status, selects observation positions at preset positions in the first observation window 114 and the second observation window 115 respectively, and divides them into 6 square detection areas;

[0053] S5: Divide the 6 small squares of the first observation window 114 into b equal parts along the X direction and a equal parts along the Y direction. Each small square generates a*b detection fields, and the entire first observation window 114 generates 6*a*b detection fields.

[0054] S6: Divide the six small squares of the second observation window 115 into B equal parts along the X direction and A equal parts along the Y direction. Each small square generates A*B detection fields of view, and the entire second observation window 115 generates 6*A*B detection fields of view.

[0055] S7: After the counting plate is loaded in the instrument, the system automatically positions itself to the origin and scans and detects each subdivided area of ​​the first observation window 114 and the second observation window 115 along the X and Y axes in the order of 1-2-3-4-5-6.

[0056] S8: Test complete. Open the instrument door, remove the counting plate, and the test is complete.

[0057] In this embodiment, firstly as follows Figure 3 As shown, after collecting the samples, add them to a container with the stained solution, mix thoroughly, and then use a pipette to take a certain amount of the stained mixed specimen. Then, as shown... Figure 4 As shown, place the counting plate horizontally on the table. Use a pipette to add the stained mixed specimen into the sample application port 105 and let it stand for a period of time. Then, according to the direction indicated by the arrow 109 above the counting plate, align the counting plate with the placement slot in the instrument door, and proceed as shown. Figure 5 The direction shown pushes the counting plate into the placement slot until the limiting notches 108 on both sides of the counting plate are locked by the corresponding limiting posts. The warehouse is then closed, and the instrument begins testing. Before testing, the instrument automatically determines the liquid filling status, selects observation positions at preset locations in the first observation window 114 and the second observation window 115, and divides them into 6 square detection areas (e.g., ...). Figure 6-2As shown), the six small squares of the first observation window 114 are divided into b equal parts along the X direction and a equal parts along the Y direction. Each small square generates a*b detection fields, and the entire first observation window 114 generates 6*a*b detection fields. The six small squares of the second observation window 115 are divided into B equal parts along the X direction and A equal parts along the Y direction. Each small square generates A*B detection fields, and the entire second observation window 115 generates 6*A*B detection fields. After the counting plate is loaded in the instrument, the system automatically positions itself to the origin and scans the subdivided areas of the first observation window 114 and the second observation window 115 along the X and Y axes in the order of 1-2-3-4-5-6 (which can be designed as 1-2-4-3-5-6, 1-4-5-6-3-2, 1-4-5-2-3-6, etc., depending on the detection requirements). Figure 6-4 (As shown), finally, the test is completed, the instrument door is opened, the counting plate is taken out, and the test is finished;

[0058] The above description discloses only one preferred embodiment of the present invention, and should not be construed as limiting the scope of the present invention. Those skilled in the art will understand that all or part of the processes of the above embodiments can be implemented, and equivalent changes made in accordance with the claims of the present invention are still within the scope of the invention.

Claims

1. A siphon microchannel type formed element counting plate, characterized in that, The device includes an upper cover plate and a lower base plate, which are bonded together by double-sided adhesive and located on the upper surface of the lower base plate. A first detection chamber and a second detection chamber are disposed between the upper cover plate and the lower base plate. A sample inlet is disposed at the center of the upper cover plate. The first detection chamber and the second detection chamber are respectively located on both sides of the sample inlet. A first overflow outlet is disposed at the end of the first detection chamber away from the sample inlet, and a second overflow outlet is disposed at the end of the second detection chamber away from the sample inlet. Both the first detection chamber and the second detection chamber have a gradually changing elliptical structure. The guide edges of the first detection chamber and the second detection chamber both change from narrow to wide along the gradually changing elliptical structure. The thickness of the second detection chamber is greater than the thickness of the first detection chamber. The length of the first detection chamber is less than the length of the second detection chamber. The width of the first detection chamber is greater than the width of the second detection chamber.

2. The siphon microchannel organic component counting plate as described in claim 1, characterized in that, Limiting notches are provided on both sides of the upper cover plate and the lower base plate. A marking arrow is provided on the surface of the upper cover plate. The marking arrow is located at the end of the first detection cavity away from the sample inlet. Arc-shaped guide edges are provided on both sides of the upper cover plate and the lower base plate near the end of the marking arrow.

3. The siphon microchannel organic component counting plate as described in claim 2, characterized in that, A spacer block is provided in the middle of the sample dispensing port, which divides the sample dispensing port into a first sample dispensing area and a second sample dispensing area. The first sample dispensing area is connected to the first detection cavity, and the second sample dispensing area is connected to the second detection cavity.

4. The siphon microchannel organic component counting plate as described in claim 3, characterized in that, The height of the spacer block is lower than the upper surface of the sample dispensing port.

5. The siphon microchannel organic component counting plate as described in claim 4, characterized in that, The opening size of the first overflow port is smaller than the opening size of the second overflow port.

6. The siphon microchannel organic component counting plate as described in claim 5, characterized in that, The upper surface of the cover plate is provided with a first recess and a second recess. The first recess is located in the middle of the flow channel of the first detection cavity and forms a first observation window in the first detection cavity. The second recess is located in the middle of the flow channel of the second detection cavity and forms a second observation window in the second detection cavity.

7. The siphon microchannel organic component counting plate as described in claim 6, characterized in that, The depth of the first depression is greater than the depth of the second depression.

8. A method of using a siphon microchannel type formed element counting plate, applied to the siphon microchannel type formed element counting plate as described in claim 7, characterized in that, Includes the following steps: After collecting the samples, add them to a container with the stained solution, mix thoroughly, and then use a pipette to take a certain amount of the stained mixed specimen. Place the counting chamber horizontally on the table, use a pipette to add the stained mixed specimen into the sample inlet, and let it stand for a period of time; Following the direction of the arrow above the counting plate, align the counting plate with the placement slot in the instrument compartment and push the counting plate into the placement slot until the limiting notches on both sides of the counting plate are locked by the corresponding limiting posts. Close the compartment and the instrument will begin testing. Before the test, the instrument automatically determines the liquid filling status, selects observation positions at preset positions in the first and second observation windows respectively, and divides them into 6 square detection areas; Divide the six small squares of the first observation window into b equal parts along the X direction and a equal parts along the Y direction. Each small square generates a*b detection fields of view, and the entire first observation window generates 6*a*b detection fields of view. Divide the six small squares of the second observation window into B equal parts along the X direction and A equal parts along the Y direction. Each small square generates A*B detection fields of view, and the entire second observation window generates 6*A*B detection fields of view. After the counting plate is loaded in the instrument, the system automatically positions itself at the origin and scans and detects each subdivided area of ​​the first observation window and the second observation window along the X and Y axes in the order of 1-2-3-4-5-6. The test is complete. The instrument door is opened, the counting plate is removed, and the test is finished.

Images