Ilex asprella tissue culture seedling cultivation device and method based on twig cuttage
By combining the rotating block component and the flipping soil-covering component, the problems of difficulty in removing seedlings and root spread during the cutting of Ilex chinensis are solved, achieving efficient and safe seedling transplantation and improving survival rate and transplanting efficiency.
Patent Information
- Application Number
- CN202511418688.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-30
- Publication Date
- 2025-11-28
AI Technical Summary
During the softwood cutting propagation of Ilex chinensis, it is difficult to remove the seedlings after the seedlings have been cultivated, and the seedlings are easily damaged. In addition, the roots are easily separated from the soil, resulting in a low survival rate.
The cultivation device, which includes a rotating block assembly, a soil-tightening assembly, and a ring-binding assembly, achieves synchronous transplanting of seedlings and soil through the coordinated action of rotating soil-inserting rods, lifting soil-lifting rods, and soil-tightening rods, thus protecting tender buds and roots from damage.
It significantly improved the survival rate and transplanting efficiency of *Ilex crenata* cuttings, reduced physical damage to the root system, ensured root integrity, and shortened the seedling recovery period.
Smart Images
Figure CN121014388A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of cutting propagation technology, and in particular to a device and method for cultivating tissue culture seedlings of *Ilex crenata* based on softwood cuttings. Background Technology
[0002] Cutting propagation is a method of propagation that uses the plant's vegetative organs (roots, stems, and leaves) to form independent plants. Plants propagated by this method grow faster than seedlings and can be cultivated into large seedlings in a short time. *Ilex chinensis*, the root of the holly family plant *Ilex chinensis*, has the effects of clearing heat and detoxifying, promoting body fluid production, relieving sore throat, dispersing blood stasis and relieving pain. It is used for influenza with high fever, acute tonsillitis, and pharyngitis. Pharmacological studies have also shown that it can dilate vascular bundles, increase coronary blood flow, and strengthen myocardial contractility, making it an irreplaceable medicinal material in prescriptions.
[0003] Currently, the existing process of softwood cutting propagation of *Rhizophora stylosa* requires seedling cultivation. After the seedlings are cultivated, when removing them, firstly, the potting soil and cuttings easily get stuck inside the seedling tray, making it difficult for workers to remove the seedlings and reducing transplanting efficiency. Secondly, the seedlings are easily damaged during removal, and the lack of proper protection for sprouting seedlings reduces the survival rate after transplanting. Finally, during the transplanting process, the roots of the *Rhizophora stylosa* cuttings are easily separated from the original soil, exposing the root system after rooting, directly leading to a significant reduction in the survival rate of the transplanted *Rhizophora stylosa* seedlings. Summary of the Invention
[0004] To overcome the shortcomings of the prior art, the present invention provides a device and method for cultivating tissue culture seedlings of *Ilex crenata* based on softwood cuttings.
[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a device and method for cultivating *Rhizophora speciosa* tissue culture seedlings based on softwood cuttings, comprising a cultivation tray with cultivation holes and a base frame. The top of the cultivation tray is provided with a rotating block assembly for transplanting *Rhizophora speciosa* cuttings in the cultivation holes. A soil-inserting rod in the rotating block assembly drives the soil around the roots of the *Rhizophora speciosa* cuttings to rotate into blocks within the cultivation holes. A soil-lifting rod in the rotating block assembly lifts the soil around the roots of the *Rhizophora speciosa* cuttings within the cultivation holes for transplanting. A protective column in the rotating block assembly surrounds and protects the *Rhizophora speciosa* cuttings during the transplanting process. A soil-closing and turning assembly is provided around the bottom of the protective column to bind the soil around the roots of the *Rhizophora speciosa* cuttings. After the soil-closing rod in the soil-closing and turning assembly is turned over, it inserts and locks the soil around the roots of the *Rhizophora speciosa* cuttings. The soil-closing and turning assembly includes a turning frame and an arc-shaped toothed plate. The rotating frame is equipped with a ring-shaped binding component for clamping and fixing the cuttings of *Rhizophora stylosa*. The arc-shaped binding plate in the ring-shaped binding component binds and transplants cuttings of different sizes of *Rhizophora stylosa*. The top of the cultivation tray is equipped with a rotating linkage component that drives the soil-inserting rod to rotate. The base frame is equipped with a moving reciprocating component that drives the soil-binding rod to move.
[0006] As a preferred embodiment of the present invention, the rotating block assembly further includes a base ring and a rotating disk. Several connecting plates are evenly installed on the inner side of the base ring. Each connecting plate is movably connected to a soil lifting rod. An arc-shaped swing arm is fixedly installed at the top of the soil lifting rod. A rotating arm is movably connected to the top of the arc-shaped swing arm through a rotating shaft. A rotating disk is movably arranged at the top center of the base ring. The rotating arm is movably connected to the bottom of the rotating disk through a rotating shaft.
[0007] Several soil-inserting rods are evenly installed around the bottom of the rotating disk, and the soil-inserting rods are all movable inside the base ring. Several triangular soil-lifting plates are evenly installed around the bottom of the soil-lifting rods, and the triangular soil-lifting plates are movable in the soil of the cultivation holes. The rotating arm and the arc-shaped swing arm are both movable between the base ring and the rotating disk. The arc-shaped swing arm has a protective column fixedly installed at the end away from the connecting plate.
[0008] As a preferred embodiment of the present invention, the soil-tightening assembly further includes a first bevel gear movable inside the tilting frame. Several support plates are evenly installed around the top of the base ring. An arc-shaped rack plate is fixedly installed at the top of the support plate. A support shaft is movably arranged around the inside of the protective column. A drive gear is fixedly installed at the top of the support shaft, and the drive gear meshes with the arc-shaped rack plate. The tilting frame is fixedly installed at the bottom of the protective column by bolts. The bottom of the support shaft extends through into the inside of the tilting frame, and the first bevel gear is fixedly installed at the bottom of the support shaft in the tilting frame.
[0009] The tilting frame has a tilting shaft inside, and a tilting plate is movably connected to the outside of the tilting frame via a rotating shaft. A second bevel gear is fixedly installed at one end of the tilting shaft, and the second bevel gear is movably meshed with the first bevel gear. The other end of the tilting shaft passes through the tilting frame and is installed on one of the tilting plates. A soil-binding rod is fixedly installed at the end of the tilting plate away from the tilting frame, and a connecting rod is fixedly installed between the soil-binding rods. The tilting plate, soil-binding rod, and connecting rod are all movable outside the tilting frame.
[0010] As a preferred embodiment of the present invention, the annular binding assembly further includes a base rod and a binding rod. The base rod is fixedly installed on the side of the flip frame near the base ring. The binding rod is movably connected to the end of the base rod away from the flip frame. The base rod is fixedly installed with a telescopic spring inside. The top of the telescopic spring is fixedly installed with a binding rod. The binding rod is fixedly installed with an arc-shaped binding plate at the end away from the flip frame.
[0011] The rotating linkage assembly includes a rotating pulley, a transmission belt, and a servo motor. The base frame is equipped with a U-shaped movable rod that is movable on the top of the cultivation rack. An electric telescopic rod is fixedly installed on the top of the U-shaped movable rod, and an arc-shaped fixed plate is fixedly installed on the bottom of the electric telescopic rod. Several rotating pulleys are movably connected to the bottom of the arc-shaped fixed plate through a rotating shaft. The bottom of the rotating pulleys is installed at the top center of the rotating disk, and a transmission belt is connected to the outer periphery of the rotating pulleys. A servo motor is connected to the top of the arc-shaped fixed plate through a motor plate, and the output end of the servo motor is connected to the top center of one of the rotating pulleys through a rotating shaft. An L-shaped mounting rod is connected to the arc-shaped fixed plate, and the bottom end of the L-shaped mounting rod is fixedly installed at the top of the base ring.
[0012] As a preferred embodiment of the present invention, the reciprocating assembly includes a stepper motor installed at one end inside the base frame, and a screw connected to the output end of the stepper motor. A moving block is threaded through the screw. A U-shaped movable rod is fixedly installed at the top of the moving block. A movable groove is opened at the top of the base frame, and the bottom of the U-shaped movable rod is movably inserted through the movable groove. A limiting rod is fixedly installed inside the base frame, and both ends of the moving block are movably inserted through the limiting rod. A drainage hole is opened at the bottom of the cultivation hole in the cultivation tray. A water collection rack with an L-shaped water outlet pipe is connected to the bottom of the cultivation tray. The cultivation tray is fixedly installed at the top of the base frame by a support foot.
[0013] As a preferred technical solution of the present invention, a method for using a tissue culture seedling cultivation device for *Ilex crenata* based on softwood cuttings includes the following steps: Step 1: Fill the cultivation holes in the cultivation tray with moist and loose soil containing plant rooting nutrient solution. Insert the newly grown, green, tender Ilex crenata branches into the cultivation holes in the cultivation tray. During the growth of the cuttings, spray with hormone solution, provide shade and sun protection, and control temperature and humidity. When the roots of the cuttings develop in the soil of the cultivation holes and new buds appear, the Ilex crenata cuttings can be transplanted. Step 2: Stop spraying five days before transplanting to allow the soil substrate in the cultivation holes to become semi-dry. This forces the roots of the *Ilex crenata* cuttings to "seek water" and enhance their resistance. Remove the roots of the *Ilex crenata* cuttings along with the soil in the cultivation holes simultaneously, without washing the roots. Retain the original soil substrate surrounding the roots. Specifically, insert the insertion rod and lifting rod under the arc-shaped fixing plate into the soil in the cultivation holes. The drive belt drives the rotating disc under the rotating pulley to rotate. The insertion rod under the rotating disc causes the soil to rotate within the cultivation holes, facilitating transplanting. The rotating disc, through the rotating plate, drives the arc-shaped swing arm on the connecting plate to retract, simultaneously causing the lifting rod to rotate within the soil. This allows the triangular lifting plate of the lifting rod to enter the soil, facilitating the subsequent simultaneous transplanting and removal of the cutting roots and soil. Step 3: The arc-shaped swing arm retracts and moves around the protective column to form a protective barrier around the cutting seedling. During the rotation around the protective column, the active gear meshes with the arc-shaped rack and pinion plate to drive the support shaft to rotate. The support shaft drives the soil-closing rod on the flipping plate to rotate 180° through the first and second bevel gears, so that the soil-closing rod is inserted into the raised soil. The soil-closing rod and the flipping plate wrap around the soil, keeping the roots of the cutting seedling adhered to the soil. This ensures that the roots of the cutting seedling are fully wrapped with the original soil and does not become loose. Step 4: During the rotation and retraction of the arc-shaped swing arm, the arc-shaped binding plate connected to the flipping frame under the protective column clamps the bottom of the *Ilex crenata* cutting. The telescopic spring on the base rod, in conjunction with the binding rod, provides limiting support to the bottom of the *Ilex crenata* cutting. The *Ilex crenata* cutting, its roots, and the original soil in the cultivation hole become a single unit during transplanting, achieving a complete transplant of the *Ilex crenata* cutting. Compared with the prior art, the beneficial effects that this invention can achieve are: 1. In this invention, the rotating arm in the rotating block assembly drives the arc-shaped swing arm on the connecting plate to rotate. During the rotation of the rotating disk, the soil-inserting rods distributed in the bottom array rotate synchronously. The soil-inserting rods penetrate into the soil at the bottom of the cultivation hole. Their rotation effectively stirs and loosens the soil in the hole, while simultaneously causing the root substrate soil and root system of the *Ilex crenata* cuttings to rotate synchronously. This effectively breaks the adhesion between the soil and the hole wall, significantly reducing the resistance to subsequent seedling lifting. The overall rotation and loosening of the soil-inserting rods and the self-rotation and wrapping action of the lifting rods work together to act on the soil and root system complex in the cultivation hole.
[0014] 2. In this invention, the rotating swing arm in the rotating block assembly moves to the end of its stroke, synchronously linking with the support action on the flipping frame. Under the synergistic effect of the telescopic spring inside the base rod, the arc-shaped binding plate at the top of the binding rod adaptively and gently binds the base of the stem of the *Ilex crenata* cutting, ensuring the stability of the relative position between the above-ground plant and the underground root ball of the *Ilex crenata* cutting. This prevents the roots inside the root ball from shifting or separating from the soil due to plant swaying during the lifting process. The arc-shaped binding plate design increases the contact area, and combined with the elastic buffer provided by the spring, significantly reduces the risk of crushing or abrasion to the base of the fragile stem, allowing the *Ilex crenata* cutting and the soil in the cultivation hole to be removed and transplanted simultaneously as a whole.
[0015] 3. In this invention, the rotation of the arc-shaped swing arm in the rotating block assembly on the connecting plate drives the soil lifting rod connected to it to rotate. A specially made triangular soil lifting plate is fixed at the bottom of the soil lifting rod. As the soil lifting rod rotates, the triangular soil lifting plate acts like a miniature rotary tiller, precisely rotating into and wrapping the soil layer located in the core area of the root zone of the cutting, which has been initially loosened by the insertion rod. The insertion rod and the soil lifting rod rise synchronously. The insertion rod is responsible for overcoming the soil resistance at the bottom and sides, while the triangular soil lifting plate acts like a "basket" or "anchor," firmly supporting and lifting the core soil clump and the root system of the *Ilex crenata* cutting within it.
[0016] 4. In this invention, the rotation of the block components ensures the integrity of the soil substrate and the root system of the *Ilex chinensis* cuttings within the cultivation holes is removed as a whole, forming a "root ball". This avoids the severe fragmentation or root breakage that may occur with traditional methods, greatly improving the transplanting efficiency of a single operation. The rotation of the soil-inserting rod achieves overall loosening, and the rotation of the lifting rod achieves the core wrapping of the soil and roots, significantly reducing physical damage to the fragile root system and facilitating the rapid recovery and survival of the seedlings after transplanting.
[0017] 5. In this invention, when the arc-shaped swing arm rotates around the soil-lifting rod on the connecting plate, the arc-shaped swing arm synchronously drives the rotation and retraction movement around the protective column. As they move, these arc-shaped arms approach each other and eventually close tightly around the protective column, forming a rigid, cylindrical or cage-like annular protective structure. This annular structure, composed of the closed arc-shaped swing arms, precisely surrounds and isolates the main body of the *Ilex cornuta* cuttings located directly below the protective column in space.
[0018] 6. In this invention, a robust physical barrier is established between the protective column and the external environment during the critical stage of transplanting. This proactively prevents accidental scratching, collision, squeezing, or entanglement damage to the newly sprouted, highly fragile buds, shoots, and tender branches of the *Ilex crenata* cuttings caused by disturbances from external mechanical parts or operations during transplanting. A small, safe gap can be maintained between the inner wall of the protective column and the buds and branches, achieving non-contact protection and improving the safety protection effect for the *Ilex crenata* cuttings.
[0019] 7. In this invention, the soil-closing rod in the soil-closing assembly is inserted into the extracted soil and the roots of the *Ilex crenata* cuttings. The flipping action of the soil-closing rod, combined with the limiting action of the connecting rod, effectively compacts any loose soil at the bottom of the root ball and forcibly wraps and gathers the original cultivation soil substrate tightly around the roots of the *Ilex crenata* cuttings, forming a compact root ball that is not easily dispersed. This maximizes the maintenance of the natural contact interface between the roots and the original soil, which is key to the rapid recovery of water and nutrient absorption after transplanting. The root ball is reinforced through mechanical action rather than simply... This method of root disturbance significantly reduces physical damage to the delicate and fragile capillary roots, overcoming the problem of loose and detached root balls at the bottom during traditional transplanting. It ensures that the root ball remains highly intact throughout the entire process from lifting and transferring to planting in the new hole. The compact root ball greatly reduces root exposure and water loss during transplanting, allowing for faster establishment of effective water and nutrient absorption in the new environment, significantly shortening the recovery period, and directly and significantly improving the survival rate and initial growth vigor of *Ilex crenata* cuttings after transplanting, thus greatly increasing the survival rate of *Ilex crenata* cuttings after transplanting. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the structure of the incubation disc of the present invention; Figure 3 This is a schematic diagram of the arc-shaped fixing plate of the present invention; Figure 4 This is a schematic diagram of the structure of the base frame of the present invention; Figure 5 This is a schematic diagram of the structure of the base ring of the present invention; Figure 6 This is a schematic diagram of the structure surrounding the protective column of the present invention; Figure 7 This is a schematic diagram of the rotating disk of the present invention; Figure 8 This is a schematic diagram of the arc-shaped swing arm of the present invention; Figure 9 for Figure 8 Enlarged view of the structure at point A in the middle.
[0021] The components include: 10. Cultivation tray; 11. Cultivation holes; 12. Base frame; 13. Support feet; 14. Stepper motor; 15. Screw; 16. Water collection frame; 17. Movable trough; 18. Limiting rod; 19. L-shaped water outlet pipe; 20. Base ring; 21. Rotating disk; 22. Connecting plate; 23. Soil lifting rod; 24. Arc-shaped swing arm; 25. Rotating arm; 26. Soil insertion rod; 27. Triangular soil lifting plate; 30. Surrounding protective column; 31. Support shaft; 32. Drive gear; 33. Support plate; 34. 35. Arc-shaped rack plate; 36. First bevel gear; 40. Second bevel gear; 41. Tilting frame; 42. Base rod; 43. Binding rod; 44. Telescopic spring; 45. Arc-shaped binding plate; 46. Connecting rod; 47. Tilting shaft; 48. Tilting plate; 50. Soil clamping rod; 51. Arc-shaped fixing plate; 52. Rotating pulley; 53. Transmission belt; 54. Servo motor; 55. U-shaped movable rod; 56. Electric telescopic rod; 57. Motor plate; 58. L-shaped mounting rod; 69. Moving block; 60. Drainage hole. Detailed Implementation
[0022] To make the technical means, creative features, and achieved objectives and effects of this invention easier to understand, the invention is further described below with reference to specific embodiments. However, the following embodiments are merely preferred embodiments of this invention and not all of them. Other embodiments obtained by those skilled in the art based on the embodiments described herein without creative effort are all within the protection scope of this invention. Unless otherwise specified, the experimental methods in the following embodiments are conventional methods, and the materials and reagents used in the following embodiments are commercially available unless otherwise specified.
[0023] Example: Figure 1 , Figure 2 , Figure 3 and Figure 5 As shown, it includes a cultivation tray 10 with cultivation holes 11 and a base frame 12. The top of the cultivation tray 10 is equipped with a rotating block assembly for transplanting the *Rhizophora stylosa* cuttings in the cultivation holes 11. The soil insertion rod 26 in the rotating block assembly drives the soil around the roots of the *Rhizophora stylosa* cuttings to rotate into blocks within the cultivation holes 11. The soil lifting rod 23 in the rotating block assembly lifts the soil around the roots of the *Rhizophora stylosa* cuttings within the cultivation holes 11 for transplanting. The surrounding protective column 30 in the rotating block assembly surrounds and protects the *Rhizophora stylosa* cuttings during the transplanting process. See Figure 3 , Figure 5 , Figure 6 , Figure 7 and Figure 8The rotating block assembly also includes a base ring 20 and a rotating disk 21. Three connecting plates 22 are evenly installed on the inner side of the base ring 20. Each connecting plate 22 is movably connected to a lifting rod 23, and an arc-shaped swing arm 24 is fixedly installed at the top of the lifting rod 23. A rotating arm 25 is movably connected to the top of the arc-shaped swing arm 24 via a pivot. A rotating disk 21 is movably positioned at the top center of the base ring 20. The rotating arm 25 is movably connected to the bottom of the rotating disk 21 via a pivot. Three soil-inserting rods 26 are evenly installed around the bottom of the rotating disk 21, and the soil-inserting rods 26 are all movably positioned inside the base ring 20. Several triangular lifting plates 27 are evenly installed around the bottom of the lifting rods 23. The soil plate 27 moves within the soil of the cultivation hole 11. The triangular soil lifting plate 27 rotates within the soil, ensuring sufficient contact between the triangular soil lifting plate 27 and the soil, increasing the contact area between the triangular soil lifting plate 27 and the soil, making it easier to remove the soil from the cultivation hole 11 for transplanting. The rotating arm 25 and the arc-shaped swing arm 24 both move between the base ring 20 and the rotating disk 21. The arc-shaped swing arm 24 has a protective post 30 fixedly installed at the end away from the connecting plate 22. The protective post 30 is made of plastic and effectively protects the periphery of the *Ilex crenata* cuttings by surrounding them, preventing damage to the newly emerging buds of the *Ilex crenata* cuttings during transplanting and reducing the transplant survival rate.
[0024] See Figure 2 , Figure 3 , Figure 5 , Figure 6 , Figure 7 and Figure 8 The electric telescopic rod 55 under the U-shaped movable rod 54 drives the arc-shaped fixed plate 50 to descend. The soil insertion rod 26 and the soil lifting rod 23 under the arc-shaped fixed plate 50 are inserted into the soil in the cultivation hole 11. The servo motor 53 on the motor plate 56 drives the rotating pulley 51 to rotate. The rotating pulley 51 drives all the rotating pulleys 51 at the bottom of the arc-shaped fixed plate 50 to rotate synchronously through the transmission belt 52. The rotating pulleys 51 drive the rotating disk 21 at the bottom of the arc-shaped fixed plate 50 to rotate. During the rotation of the rotating disk 21, the arc-shaped swing arm 24 on the connecting plate 22 is driven to rotate through the rotating arm 25. During the rotation of the rotating disk 21, the soil-inserting rod 26 at the bottom rotates, causing the soil in the cultivation hole 11 and the roots of the *Ilex crenata* cuttings to rotate synchronously. This facilitates the subsequent removal of the soil and *Ilex crenata* cuttings from the cultivation hole 11 for transplanting. Meanwhile, the arc-shaped swing arm 24 rotates on the connecting plate 22, causing the lifting rod 23 to rotate. The triangular lifting plate 27 at the bottom of the lifting rod 23 rotates within the soil layer, allowing it to enter the soil. This facilitates the subsequent removal of the soil and *Ilex crenata* cuttings from the cultivation hole 11 as a whole, improving the overall transplanting efficiency of the soil and roots of the *Ilex crenata* cuttings in the cultivation hole 11.
[0025] See Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9 A soil-tightening assembly is installed around the bottom of the protective column 30 to bind the soil around the roots of the *Ilex crenata* cuttings. The soil-tightening rod 48 within the assembly flips over to insert and lock the soil around the roots of the cuttings. The assembly includes a flipping frame 40 and an arc-shaped rack plate 34. It also includes a first bevel gear 35 movable inside the flipping frame 40. Several support plates 33 are evenly installed around the top of the base ring 20. The arc-shaped rack plate 34 is fixedly installed at the top of the support plates 33. A support shaft 31 is movably arranged around the inside of the protective column 30. A drive gear 32 is fixedly installed at the top of the support shaft 31, and the drive gear 32 meshes with the arc-shaped rack plate 34. The flipping frame 40 is bolted to the bottom of the protective column 30. The bottom of the support shaft 31 extends through the interior of the flipping frame 40. The rotation of the drive gear 32 drives the support shaft 31 to move synchronously. The bottom of the support shaft 31 is fixedly installed with a first bevel gear 35 in the tilting frame 40. The tilting frame 40 is movably installed with a tilting shaft 46 inside. The tilting frame 40 is movably connected with a tilting plate 47 through the shaft. A second bevel gear 36 is fixedly installed at one end of the tilting shaft 46, and the second bevel gear 36 is movably meshed with the first bevel gear 35. The other end of the tilting shaft 46 passes through the tilting frame 40 and is installed on one of the tilting plates 47. A soil clamping rod 48 is fixedly installed at the end of the tilting plate 47 away from the tilting frame 40, and a connecting rod 45 is fixedly installed between the soil clamping rods 48. The connecting rod 45 drives the tilting plates 47 on both sides of the tilting frame 40 to move synchronously. The tilting plate 47, soil clamping rod 48 and connecting rod 45 are all movable outside the tilting frame 40. The tilting frame 40 has a square structure.
[0026] See Figure 3 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9After the rotating disc 21 rotates the soil in the cultivation hole 11 a certain distance via the soil insertion rod 26, the rotating disc 21 stops rotating. The electric telescopic rod 55 drives the arc-shaped fixing plate 50 to rise. The soil in the cultivation hole 11 and the roots of the *Ilex crenata* cutting are lifted and transplanted as a whole using the soil lifting rod 23 and the soil insertion rod 26. When the arc-shaped fixing plate 50 rises to a certain height, the rotating disc 21 is driven to rotate again. When the arc-shaped swing arm 24 rotates around the soil lifting rod 23 on the connecting plate 22, the arc-shaped swing arm 24 simultaneously drives the rotation and retraction around the protective column 30, forming a ring around the protective column 30 to protect the entire *Ilex crenata* cutting, effectively preventing damage to the newly emerging buds and branches of the *Ilex crenata* cutting during transplanting.
[0027] See Figure 3 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9 When the arc-shaped swing arm 24 rotates, the drive gear 32 around the top of the protective column 30 meshes with the arc-shaped rack plate 34 on the support plate 33. The drive gear 32 rotates due to the meshing, and drives the support shaft 31 around the protective column 30 to rotate. The support shaft 31 then drives the first bevel gear 35 inside the flipping frame 40 to rotate synchronously. The first bevel gear 35 meshes with the second bevel gear 36 to drive the flipping shaft 46 to rotate. The flipping shaft 46 then drives the flipping plates 47 on both sides to continuously flip. The soil-binding rod 48 on the flipping plate 47, together with the connecting rod 45, is inserted into the bottom of the soil. The soil-binding rod 48 is inserted into the soil and the roots of the *Ilex crenata* cuttings, so that the original soil of the roots of the *Ilex crenata* cuttings is wrapped in the root system, which greatly improves the survival rate of the transplanted *Ilex crenata* cuttings.
[0028] See Figure 3 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9 The rotating frame 40 is equipped with an annular binding assembly for clamping and fixing the cuttings of *Ilex crenata*. The annular binding assembly has an arc-shaped binding plate 44 for binding and transplanting cuttings of different sizes. The annular binding assembly also includes a base rod 41 and a binding rod 42. The rotating frame 40 has a base rod 41 fixedly installed on the side near the base ring 20. The binding rod 42 is movably connected to the end of the base rod 41 away from the rotating frame 40. The base rod 41 has a telescopic spring 43 fixedly installed inside. The top of the telescopic spring 43 has a binding rod 42 fixedly installed. The binding rod 42 has an arc-shaped binding plate 44 fixedly installed at the end away from the rotating frame 40. The arc-shaped binding plate 44 is made of plastic.
[0029] See Figure 1 , Figure 3 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9 When the rotating swing arm drives the protective column 30 to achieve ring protection for the *Rhizophora stylosa* cuttings, that is, when the rotating swing arm rotates to the end of the arc-shaped rack plate 34 near the support plate 33, the base rod 41 and binding rod 42 on the flipping frame 40 will also rotate synchronously to the bottom of the *Rhizophora stylosa* cuttings. Then, the arc-shaped binding plate 44 at the top of the binding rod 42, together with the telescopic spring 43 in the base rod 41, supports and limits the *Rhizophora stylosa* cuttings of different sizes. When the *Rhizophora stylosa* cuttings are taken out of the cultivation hole 11 for transplanting, the arc-shaped binding plate 44 can limit and support the *Rhizophora stylosa* cuttings, so that the *Rhizophora stylosa* cuttings and the soil in the cultivation hole 11 can be taken out and transplanted synchronously as a whole.
[0030] See Figure 1 , Figure 2 , Figure 3 , Figure 5 and Figure 6 The top of the cultivation tray 10 is equipped with a rotary linkage assembly that drives the soil insertion rod 26 to rotate. The rotary linkage assembly includes a rotary pulley 51, a transmission belt 52, and a servo motor 53. The base frame 12 is equipped with a U-shaped movable rod 54 that is movable on the top of the cultivation rack. An electric telescopic rod 55 is fixedly installed on the top of the U-shaped movable rod 54. An arc-shaped fixed plate 50 is fixedly installed on the bottom of the electric telescopic rod 55. The electric telescopic rod 55 realizes the lifting and lowering movement of the arc-shaped fixed plate 50. Several rotary pulleys are movably connected to the bottom of the arc-shaped fixed plate 50 through a rotating shaft. 51. The bottom of the rotating pulley 51 is installed at the top center of the rotating disk 21. A transmission belt 52 is connected to the outer periphery of the rotating pulley 51. The top of the arc-shaped fixing plate 50 is connected to a servo motor 53 through a motor plate 56. The output end of the servo motor 53 is connected to the top center of one of the rotating pulleys 51 through a rotating shaft. The arc-shaped fixing plate 50 is connected to an L-shaped mounting rod 57. The bottom end of the L-shaped mounting rod 57 is fixedly installed at the top of the base ring 20. The base ring 20 is fixed to the bottom of the arc-shaped fixing plate 50 by the L-shaped mounting rod 57.
[0031] See Figure 1 , Figure 2 , Figure 3 , Figure 5 and Figure 6The servo motor 53 on the motor board 56 drives the rotating pulley 51 to rotate. The rotating pulley 51 drives all the rotating pulleys 51 at the bottom of the arc-shaped fixed plate 50 to rotate synchronously through the transmission belt 52. The rotating pulleys 51 drive the rotating disk 21 at the bottom of the arc-shaped fixed plate 50 to rotate.
[0032] See Figure 1 , Figure 2 , Figure 3 and Figure 4 The base frame 12 is equipped with a reciprocating assembly that drives the soil clamping rod 48 to move. The reciprocating assembly includes a stepper motor 14 installed at one end inside the base frame 12, and a screw 15 is connected to the output end of the stepper motor 14. A moving block 58 is threaded through the screw 15. A U-shaped movable rod 54 is fixedly installed at the top of the moving block 58. A movable groove 17 is opened at the top of the base frame 12, and the bottom of the U-shaped movable rod 54 is movably inserted through the movable groove 17. A limit rod 18 is fixedly installed inside the base frame 12, and both ends of the moving block 58 are movably inserted through the limit rod 18. The cultivation tray 10 has a drainage hole 60 at the bottom of the cultivation hole 11. A water collection rack 16 with an L-shaped water outlet pipe 19 is connected to the bottom of the cultivation tray 10. The cultivation tray 10 is fixedly installed at the top of the base frame 12 by a support foot 13.
[0033] See Figure 1 , Figure 2 , Figure 3 and Figure 4 After the top row of *Rhizophora stylosa* cuttings in the cultivation tray are removed and transplanted, the moving block 58 on the screw 15 is driven by the stepper motor 14 to reciprocate in the limiting rod 18. During the movement, the moving block 58 drives the U-shaped moving rod 54 through the movable groove 17 to enter the top of the next row of cultivation holes 11, thereby realizing the stable transplantation of all *Rhizophora stylosa* cuttings in the cultivation tray 10 cultivation holes 11.
[0034] Working principle: The electric telescopic rod 55 under the U-shaped movable rod 54 drives the arc-shaped fixed plate 50 to descend. The soil insertion rod 26 and the soil lifting rod 23 under the arc-shaped fixed plate 50 are inserted into the soil in the cultivation hole 11. The servo motor 53 on the motor plate 56 drives the rotating pulley 51 to rotate. The rotating pulley 51 drives all the rotating pulleys 51 at the bottom of the arc-shaped fixed plate 50 to rotate synchronously through the transmission belt 52. The rotating pulleys 51 drive the rotating disk 21 at the bottom of the arc-shaped fixed plate 50 to rotate. During the rotation of the rotating disk 21, the rotating arm 25 drives the arc-shaped swing arm 24 on the connecting plate 22 to rotate. During the rotation of the rotating disk 21, the soil-inserting rod 26 at the bottom rotates, causing the soil in the cultivation hole 11 and the roots of the *Ilex crenata* cuttings to rotate synchronously. This facilitates the subsequent removal of the soil and *Ilex crenata* cuttings from the cultivation hole 11 for transplanting. Meanwhile, the arc-shaped swing arm 24 rotates on the connecting plate 22, causing the lifting rod 23 to rotate. The triangular lifting plate 27 at the bottom of the lifting rod 23 then rotates within the soil layer, allowing the soil and *Ilex crenata* cuttings to be removed from the cultivation hole 11 as a whole, thus improving the overall transplanting efficiency of the soil and roots of the *Ilex crenata* cuttings in the cultivation hole 11.
[0035] After the rotating disc 21 rotates the soil in the cultivation hole 11 a certain distance via the soil insertion rod 26, the rotating disc 21 stops rotating. The electric telescopic rod 55 drives the arc-shaped fixing plate 50 to rise. The soil in the cultivation hole 11 and the roots of the *Ilex crenata* cutting are lifted and transplanted as a whole using the soil lifting rod 23 and the soil insertion rod 26. When the arc-shaped fixing plate 50 rises to a certain height, the rotating disc 21 is driven to rotate again. When the arc-shaped swing arm 24 rotates around the soil lifting rod 23 on the connecting plate 22, the arc-shaped swing arm 24 simultaneously drives the rotation and retraction around the protective column 30, forming a ring around the protective column 30 to protect the entire *Ilex crenata* cutting, effectively preventing damage to the newly emerging buds and branches of the *Ilex crenata* cutting during transplanting.
[0036] When the arc-shaped swing arm 24 rotates, the drive gear 32 around the top of the protective column 30 meshes with the arc-shaped rack plate 34 on the support plate 33. The drive gear 32 rotates due to the meshing, and drives the support shaft 31 inside the protective column 30 to rotate. The support shaft 31 then drives the first bevel gear 35 inside the flipping frame 40 to rotate synchronously. The first bevel gear 35 meshes with the second bevel gear 36 to drive the flipping shaft 46 to rotate. The flipping shaft 46 then drives the flipping plates 47 on both sides to continuously flip. The soil-binding rod 48 on the flipping plate 47, together with the connecting rod 45, is inserted into the bottom of the soil. The soil-binding rod 48 is inserted into the soil and the roots of the *Ilex crenata* cuttings, so that the original soil of the roots of the *Ilex crenata* cuttings is wrapped in the root system, which greatly improves the survival rate of the transplanted *Ilex crenata* cuttings.
[0037] When the rotating swing arm drives the holly cuttings to achieve ring protection around the protective column 30, that is, when the rotating swing arm rotates to the end of the arc-shaped rack plate 34 near the support plate 33, the base rod 41 and binding rod 42 on the flipping frame 40 will also rotate synchronously to the bottom of the holly cuttings. Then, the arc-shaped binding plate 44 at the top of the binding rod 42, together with the telescopic spring 43 in the base rod 41, supports and limits the holly cuttings of different sizes. When the holly cuttings are taken out of the cultivation hole 11 for transplanting, the arc-shaped binding plate 44 can limit and support the holly cuttings, so that the holly cuttings and the soil in the cultivation hole 11 can be taken out and transplanted synchronously as a whole.
[0038] After the top row of *Rhizophora stylosa* cuttings in the cultivation tray are removed and transplanted, the moving block 58 on the screw 15 is driven by the stepper motor 14 to reciprocate in the limiting rod 18. During the movement, the moving block 58 drives the U-shaped moving rod 54 through the movable groove 17 to enter the top of the next row of cultivation holes 11, thereby realizing the stable transplanting of all *Rhizophora stylosa* cuttings in the cultivation tray 10 cultivation holes 11.
[0039] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited thereto. Various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention.
Claims
1. A device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings, comprising a cultivation tray with cultivation holes and a base frame, characterized in that, The top of the cultivation tray is equipped with a rotating block assembly for transplanting the *Rhizophora speciosa* cuttings in the cultivation holes. The soil insertion rod in the rotating block assembly rotates the soil around the roots of the *Rhizophora speciosa* cuttings into blocks within the cultivation holes. The soil lifting rod in the rotating block assembly lifts the soil around the roots of the *Rhizophora speciosa* cuttings within the cultivation holes for transplanting. The rotating block assembly is equipped with a protective column to surround and protect the *Rhizophora speciosa* cuttings during the transplanting process. At the bottom of the protective column, there is a soil-closing assembly that binds the soil around the roots of the *Rhizophora speciosa* cuttings. After the soil-closing rod in the soil-closing assembly is flipped, it inserts and locks the soil around the roots of the *Rhizophora speciosa* cuttings. The soil-closing assembly includes a flipping frame and an arc-shaped toothed plate. The rotating frame is equipped with a ring-shaped binding component for clamping and fixing the cuttings of *Rhizophora stylosa*. The arc-shaped binding plate in the ring-shaped binding component binds and transplants cuttings of different sizes of *Rhizophora stylosa*. The top of the cultivation tray is equipped with a rotating linkage component that drives the soil-inserting rod to rotate. The base frame is equipped with a moving reciprocating component that drives the soil-binding rod to move.
2. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 1, characterized in that, The rotating block assembly also includes a base ring and a rotating disk. Several connecting plates are evenly installed on the inner side of the base ring. Each connecting plate is movably connected to a soil lifting rod. An arc-shaped swing arm is fixedly installed at the top of the soil lifting rod. A rotating arm is movably connected to the top of the arc-shaped swing arm through a rotating shaft. A rotating disk is movably set at the top center of the base ring. The rotating arm is movably connected to the bottom of the rotating disk through a rotating shaft.
3. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 2, characterized in that, Several soil-inserting rods are evenly installed around the bottom of the rotating disk, and the soil-inserting rods are all movable inside the base ring. Several triangular soil-lifting plates are evenly installed around the bottom of the soil-lifting rods, and the triangular soil-lifting plates are movable in the soil of the cultivation holes. The rotating arm and the arc-shaped swing arm are both movable between the base ring and the rotating disk. The arc-shaped swing arm has a protective column fixedly installed at the end away from the connecting plate.
4. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 3, characterized in that, The soil-tilting assembly also includes a first bevel gear movable inside the tilting frame. Several support plates are evenly installed around the top of the base ring. An arc-shaped rack plate is fixedly installed at the top of the support plate. A support shaft is movably arranged around the inside of the protective column. A drive gear is fixedly installed at the top of the support shaft, and the drive gear is movably meshed with the arc-shaped rack plate. The tilting frame is fixedly installed at the bottom of the protective column by bolts. The bottom of the support shaft extends through into the inside of the tilting frame, and the first bevel gear is fixedly installed at the bottom of the support shaft in the tilting frame.
5. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 4, characterized in that, The tilting frame has a tilting shaft inside, and a tilting plate is movably connected to the outside of the tilting frame via a rotating shaft. A second bevel gear is fixedly installed at one end of the tilting shaft, and the second bevel gear is movably meshed with the first bevel gear. The other end of the tilting shaft passes through the tilting frame and is installed on one of the tilting plates. A soil-binding rod is fixedly installed at the end of the tilting plate away from the tilting frame, and a connecting rod is fixedly installed between the soil-binding rods. The tilting plate, soil-binding rod, and connecting rod are all movable outside the tilting frame.
6. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 5, characterized in that, The ring-shaped binding assembly also includes a base rod and a binding rod. The base rod is fixedly installed on the side of the flip frame near the base ring. The binding rod is movably connected to the end of the base rod away from the flip frame. The base rod is fixedly installed with a telescopic spring inside. The top of the telescopic spring is fixedly installed with a binding rod. The binding rod is fixedly installed with an arc-shaped binding plate at the end away from the flip frame.
7. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 6, characterized in that, The rotating linkage assembly includes a rotating pulley, a transmission belt, and a servo motor. The base frame is equipped with a U-shaped movable rod that is movable on the top of the cultivation rack. An electric telescopic rod is fixedly installed on the top of the U-shaped movable rod, and an arc-shaped fixed plate is fixedly installed on the bottom of the electric telescopic rod. Several rotating pulleys are movably connected to the bottom of the arc-shaped fixed plate through a rotating shaft. The bottom of the rotating pulleys is installed at the top center of the rotating disk, and a transmission belt is connected to the outer periphery of the rotating pulleys. A servo motor is connected to the top of the arc-shaped fixed plate through a motor plate, and the output end of the servo motor is connected to the top center of one of the rotating pulleys through a rotating shaft. An L-shaped mounting rod is connected to the arc-shaped fixed plate, and the bottom end of the L-shaped mounting rod is fixedly installed at the top of the base ring.
8. The device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 7, characterized in that, The reciprocating motion assembly includes a stepper motor installed inside one end of the base frame, with a screw connected to the output end of the stepper motor. A moving block is threaded through the screw. A U-shaped movable rod is fixedly installed at the top of the moving block. A movable groove is opened at the top of the base frame, and the bottom of the U-shaped movable rod moves through the movable groove. A limit rod is fixedly installed inside the base frame, and both ends of the moving block move through the limit rod. A drainage hole is opened at the bottom of the cultivation tray inside the cultivation hole. A water collection rack with an L-shaped water outlet pipe is connected to the bottom of the cultivation tray. The cultivation tray is fixedly installed at the top of the base frame by support feet.
9. The method of using the device for cultivating *Ilex crenata* tissue culture seedlings based on softwood cuttings according to claim 8, characterized in that, It includes the following steps: Step 1: Fill the cultivation holes in the cultivation tray with moist and loose soil containing plant rooting nutrient solution. Insert the newly grown, green, tender Ilex crenata branches into the cultivation holes in the cultivation tray. During the growth of the cuttings, spray with hormone solution, provide shade and sun protection, and control temperature and humidity. When the roots of the cuttings develop in the soil of the cultivation holes and new buds appear, the Ilex crenata cuttings can be transplanted. Step 2: Stop spraying five days before transplanting to allow the soil substrate in the cultivation holes to become semi-dry. This forces the roots of the *Ilex crenata* cuttings to "seek water" and enhance their resistance. Remove the roots of the *Ilex crenata* cuttings along with the soil in the cultivation holes simultaneously, without washing the roots. Retain the original soil substrate surrounding the roots. Specifically, insert the insertion rod and lifting rod under the arc-shaped fixing plate into the soil in the cultivation holes. The drive belt drives the rotating disc under the rotating pulley to rotate. The insertion rod under the rotating disc causes the soil to rotate within the cultivation holes, facilitating transplanting. The rotating disc, through the rotating plate, drives the arc-shaped swing arm on the connecting plate to retract, simultaneously causing the lifting rod to rotate within the soil. This allows the triangular lifting plate of the lifting rod to enter the soil, facilitating the subsequent simultaneous transplanting and removal of the cutting roots and soil. Step 3: The arc-shaped swing arm retracts and moves around the protective column to form a protective barrier around the cutting seedling. During the rotation around the protective column, the active gear meshes with the arc-shaped rack and pinion plate to drive the support shaft to rotate. The support shaft drives the soil-closing rod on the flipping plate to rotate 180° through the first and second bevel gears, so that the soil-closing rod is inserted into the raised soil. The soil-closing rod and the flipping plate wrap around the soil, keeping the roots of the cutting seedling adhered to the soil. This ensures that the roots of the cutting seedling are fully wrapped with the original soil and does not become loose. Step 4: During the rotation and retraction of the arc-shaped swing arm, the arc-shaped binding plate connected to the flipping frame under the protective column clamps the bottom of the *Rhizophora speciosa* cutting. The telescopic spring on the base rod, in conjunction with the binding rod, provides limiting support to the bottom of the *Rhizophora speciosa* cutting. The *Rhizophora speciosa* cutting, the roots of the cutting, and the original soil in the cultivation hole become one during transplanting, realizing the overall transplanting of the *Rhizophora speciosa* cutting.