A composition having anti-inflammatory and anti-allergic effects and use thereof

Abstract

The present application relates to a kind of compositions with anti-inflammatory and anti-allergic effects and its application, and the preparation raw materials of the composition include boletus, phyllanthus and rose apple.The present application uses steam explosion coupling heating reflux preparation to prepare the composition with boletus, phyllanthus, rose apple as preparation raw material, the method not only significantly improves extraction efficiency and biological activity, compared with traditional extraction technology, also avoids the use of organic reagent, more environmentally friendly and safe.

Description

Technical Field

[0001] This invention belongs to the field of material extraction technology and relates to a composition with anti-inflammatory and anti-allergic effects and its application. Background Technology

[0002] In recent years, multiple environmental stressors (such as dietary imbalance, pollution exposure, persistent stress, and allergen stimulation) have collectively induced the formation of a chronic pro-inflammatory microenvironment in the body, leading to immune homeostasis imbalance and increased susceptibility to allergic reactions. Studies have found that by targeting and inhibiting the release of the inflammatory mediator NO and hyaluronidase activity, it is possible not only to effectively block the cascade activation of key inflammatory signaling pathways such as NF-κB, but also to maintain the integrity of the extracellular matrix structure, thereby significantly alleviating tissue edema and allergic reactions, ultimately repairing the skin barrier and maintaining the body's immune balance.

[0003] Boletus ( Boletus Boletus edulis, a large wild fungus, is renowned as one of the "Four Famous Fungi" due to its rich nutrition and succulent texture. It is rich in flavonoids, polysaccharides, proteins, ergosterol, minerals, vitamins, amino acids, and cellulose, among other active substances, possessing antioxidant and immunomodulatory effects. However, its application in anti-allergy treatments is rarely seen.

[0004] Phyllanthus emblica ( Phyllanthus emblica L. Phyllanthus emblica is a perennial tree belonging to the Euphorbiaceae family and the Phyllanthus genus. It is a fruit that is both used for medicinal and edible purposes. Modern pharmacological studies have shown that Phyllanthus emblica is rich in active ingredients such as polyphenols, polysaccharides, vitamin C, and superoxide dismutase (SOD). Phyllanthus emblica fruit has been shown to have potential medicinal value in terms of anti-oxidation and anti-tumor effects. Its anti-inflammatory effect is significant, and it is regarded as an ideal natural anti-inflammatory drug.

[0005] prickly pear ( Rosa Roxburghii Also known as Wenxian fruit or Songchungui, it is the fruit of the perennial deciduous shrub Rosa laevigata, belonging to the Rosaceae family. Prickly pear has a rich aroma, a crisp texture, and a sour taste. Due to its high vitamin C content, it is hailed as the "King of Vitamin C" and the "Three Kings Sacred Fruit." Prickly pear possesses antioxidant, anti-inflammatory, antibacterial, and immune-regulating effects, and has broad application prospects in pharmaceuticals and cosmetics.

[0006] Natural plant-derived anti-allergic and anti-inflammatory components have gradually become a research and application hotspot due to their high safety, mild effects, and multi-target regulation. CN119700629A and CN115737754B both disclose compositions with anti-inflammatory and anti-allergic effects. Although they differ in their composition and application focus, their formulation designs share certain commonalities, including diverse components, complex formulations, and relatively simple mechanisms of action. Furthermore, these compositions all rely on organic solvent extraction systems, leading to the risk of solvent residue in the final product, which significantly limits their market application scope. SUMMARY

[0007] In view of the deficiencies of the prior art, the present application aims to provide a composition with anti-inflammatory and anti-allergic effects and application thereof.

[0008] To achieve the object of the present application, the following technical solutions are adopted:

[0009] In a first aspect, the present application provides a composition with anti-inflammatory and anti-allergic effects, the preparation raw materials of the composition comprising Boletus, Phyllanthus emblica and Rosa roxburghii.

[0010] The present application innovatively uses Boletus, Phyllanthus emblica and Rosa roxburghii to construct a composite system, which not only completely avoids the residue of organic solvents through a green extraction process to ensure product safety, but also realizes the synergistic effect of the three plants through scientific formula matching, inhibits the activity of hyaluronidase and the expression of pro-inflammatory factors (NO), and proves that the composition has both anti-inflammatory and anti-allergic effects through anti-allergic testing on a zebrafish model in vivo, which has broad application prospects.

[0011] Preferably, the preparation raw materials of the composition comprise Boletus 5-20 parts, Phyllanthus emblica 5-20 parts and Rosa roxburghii 2-10 parts by mass fraction.

[0012] The mass fraction of Boletus can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc., the mass fraction of Phyllanthus emblica can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc., and the mass fraction of Rosa roxburghii can be selected from 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc. Other specific point values within the above numerical range can be selected, which will not be described one by one here.

[0013] Preferably, the composition is prepared by a method comprising the following steps:

[0014] (1) Boletus and Phyllanthus emblica are respectively pretreated by steam explosion;

[0015] (2) Boletus after pretreatment, Phyllanthus emblica after pretreatment, Rosa roxburghii and water are mixed and refluxed to extract to obtain an extract;

[0016] (3) The extract is filtered and concentrated to obtain a concentrated solution, which is refrigerated and flocculated, filtered and dried to obtain the composition.

[0017] Compared with the traditional process, the pure water extraction technology is adopted, the use of organic solvents is completely avoided, the product safety is ensured, the demand of consumers for natural and healthy products is met, the environmental pollution risk is reduced, the process flow is simple, the energy consumption is low, and the industrial production is suitable.

[0018] Preferably, the water content of the boletus is 20-30 wt%, and the water content of the phyllanthus emblica is 25-35 wt%.

[0019] The water content of the boletus can be selected as 20 wt%, 21 wt%, 22 wt%, 23 wt%, 24 wt%, 25 wt%, 26 wt%, 27 wt%, 28 wt%, 29 wt%, 30 wt%, etc., and the water content of the phyllanthus emblica can be selected as 25 wt%, 26 wt%, 27 wt%, 28 wt%, 29 wt%, 30 wt%, 31 wt%, 32 wt%, 33 wt%, 34 wt%, 35 wt%, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0020] Preferably, the pressure of the steam explosion of the boletus is 0.4-0.8 MPa, the temperature is 140-180 ℃, and the time is 30-60 s.

[0021] The pressure can be selected as 0.4 MPa, 0.5 MPa, 0.6 MPa, 0.7 MPa, 0.8 MPa, etc., the temperature can be selected as 140 ℃, 150 ℃, 160 ℃, 170 ℃, 180 ℃, etc., and the time can be selected as 30 s, 32 s, 35 s, 38 s, 40 s, 42 s, 45 s, 48 s, 50 s, 52 s, 55 s, 58 s, 60 s, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0022] Preferably, the steam explosion of the boletus further includes drying.

[0023] Preferably, the water content of the pretreated boletus is 8 wt% or less, for example, 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0024] Preferably, the pressure of the steam explosion of the phyllanthus emblica is 0.5-0.8 MPa, the temperature is 150-180 ℃, and the time is 60-100 s.

[0025] The pressure can be selected from 0.5 MPa, 0.55 MPa, 0.6 MPa, 0.65 MPa, 0.7 MPa, 0.75 MPa, 0.8 MPa, etc., the temperature can be selected from 150 ℃, 155 ℃, 160 ℃, 165 ℃, 170 ℃, 175 ℃, 180 ℃, etc., and the time can be selected from 60 s, 65 s, 70 s, 75 s, 80 s, 85 s, 90 s, 95 s, 100 s, etc. Other specific point values within the above numerical range can be selected, and here they will not be repeated one by one.

[0026] Preferably, the Phyllanthus emblica after steam explosion also includes drying.

[0027] Preferably, the moisture content of the pretreated Phyllanthus emblica is 5-10 wt%, for example, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, etc. Other specific point values within the above numerical range can be selected, and here they will not be repeated one by one.

[0028] Preferably, the moisture content of the Phyllanthus emblica is 5-10 wt%, for example, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, etc. Other specific point values within the above numerical range can be selected, and here they will not be repeated one by one.

[0029] Preferably, the solid-liquid ratio of the reflux extraction is 1 g:(10-20) mL, wherein the specific point values in (10-20) can be selected from 10, 12, 14, 16, 18, 20, etc. Other specific point values within the above numerical range can be selected, and here they will not be repeated one by one.

[0030] Preferably, the reflux extraction time is 0.5-1 h, and the number of times is 1-3 times.

[0031] The time can be selected from 0.5 h, 0.6 h, 0.7 h, 0.8 h, 0.9 h, 1 h, etc., and the number of times can be selected from 1 time, 2 times, 3 times, etc. Other specific point values within the above numerical range can be selected, and here they will not be repeated one by one.

[0032] Preferably, the solid content of the concentrated liquid is 8-12 wt%, for example, 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, etc. Other specific point values within the above numerical range can be selected, and here they will not be repeated one by one.

[0033] Preferably, the temperature of the refrigerated flocculation is 2-6 ℃, and the time is 0.5-2 h.

[0034] The temperature can be selected as 2 ℃, 2.5 ℃, 3 ℃, 3.5 ℃, 4 ℃, 4.5 ℃, 5 ℃, 5.5 ℃, 6 ℃, etc., and the time can be selected as 0.5 h, 0.6 h, 0.8 h, 1.0 h, 1.2 h, 1.4 h, 1.6 h, 1.8 h, 2 h, etc., and other specific point values in the above numerical range can be selected, which will not be described here one by one.

[0035] In a second aspect, the present application provides a use of the composition having anti-inflammatory and anti-allergic effects according to the first aspect in the preparation of a product having anti-inflammatory and anti-allergic effects.

[0036] Preferably, the product comprises a cosmetic product.

[0037] Compared with the prior art, the present application has the following beneficial effects:

[0038] (1) The present application uses steam explosion coupled with heating reflux to prepare a composition with boletus, phyllanthus emblica and roxburgh rose as raw materials. This method not only significantly improves the extraction efficiency and biological activity, but also avoids the use of organic reagents, is more environmentally friendly and safe compared with traditional extraction techniques.

[0039] (2) The composition prepared by the preparation method of the present application has synergistic effect, the inhibition rate of the composition on hyaluronidase is significantly improved, and the expression of inflammatory factor NO and zebrafish in vivo model can be effectively inhibited. Trypsin, has stronger anti-allergic and anti-inflammatory effects. BRIEF DESCRIPTION OF DRAWINGS

[0040] Figure 1 is a zebrafish trypsin expression result graph, ### indicates compared with the normal control group, p <0.001; *** indicates compared with the model control group, p <0.001; ** indicates compared with the model control group, p <0.01; ++ indicates compared with the example 1 group, p <0.01. DETAILED DESCRIPTION

[0041] The technical solutions of the present application will be further described through specific embodiments. Those skilled in the art should understand that the embodiments are only to help understand the present application, and should not be regarded as a specific limitation on the present application.

[0042] Preparation Example 1

[0043] The present preparation example provides a preparation method of boletus extract, which comprises:

[0044] (1) Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, and steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate of <0.1 seconds. Circulate the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product.

[0045] (2) Mix the pretreated Boletus product with water at a ratio of 1 g: 15 mL, heat and reflux for 45 min, extract twice, pass through a 600 mesh filter cloth and a 5 μm filter bag to obtain the extract; concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0046] Preparation Example 2

[0047] This preparation example provides a method for preparing Phyllanthus emblica extract, the preparation method comprising:

[0048] (1) The moisture content of Phyllanthus emblica was adjusted to 25 wt%, and steam explosion treatment was carried out at 0.8 MPa and 180 ℃ for 60 s, with a pressure release rate of <0.1 seconds. The explosion was repeated once, and the product was dried to a moisture content of 8 wt%. The product was then pulverized to a mesh size of no more than 80 mesh to obtain the pretreated Phyllanthus emblica product.

[0049] (2) Mix the pretreated amla product with water at a ratio of 1 g: 15 mL, heat and reflux for 45 min, extract twice, pass through a 600 mesh filter cloth and a 5 μm filter bag to obtain the extract; concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0050] Preparation Example 3

[0051] This preparation example provides a method for preparing a prickly pear extract, the method comprising:

[0052] (1) Dry the prickly pear directly to a moisture content of 8 wt%, and pulverize it to a mesh size not exceeding 80 to obtain the pretreated prickly pear product;

[0053] (2) Mix the pretreated prickly pear product with water at a ratio of 1 g: 15 mL, heat and reflux for 45 min, extract twice, pass through a 600 mesh filter cloth and a 5 μm filter bag to obtain the extract; concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0054] Example 1

[0055] The embodiment provides a composition with anti-inflammatory and anti-allergic effects, and raw materials for preparing the composition include Boletus 10 parts, Phyllanthus emblica 10 parts and Rosa roxburghii 6 parts in mass fraction.

[0056] The preparation method comprises the following steps:

[0057] (1) The water content of Boletus is adjusted to 20 wt%, and the Boletus is cut into 2-5 cm particles, and then subjected to steam explosion treatment at 0.6 MPa and 165 DEG C for 40 s, the pressure release rate is less than 0.1 s, the cycle explosion is performed once, the dryness is performed until the water content is 4 wt%, and the crushing is performed until the particle size is not more than 80 meshes, so that a pretreated Boletus product is obtained;

[0058] (2) The water content of Phyllanthus emblica is adjusted to 25 wt%, and the Phyllanthus emblica is subjected to steam explosion treatment at 0.8 MPa and 180 DEG C for 60 s, the pressure release rate is less than 0.1 s, the cycle explosion is performed once, the dryness is performed until the water content is 8 wt%, and the crushing is performed until the particle size is not more than 80 meshes, so that a pretreated Phyllanthus emblica product is obtained;

[0059] (3) The Rosa roxburghii is directly dried until the water content is 8 wt%, and then crushed until the particle size is not more than 80 meshes, so that a pretreated Rosa roxburghii product is obtained;

[0060] (4) The pretreated Boletus product, the pretreated Phyllanthus emblica product, the pretreated Rosa roxburghii product and water are mixed, the solid-liquid ratio is 1 g:15 mL, heated reflux extraction is performed for 45 min, the extraction is performed twice, 600 mesh filter cloth and 5 μm filter bag are used, and then an extract is obtained;

[0061] (5) The extract is concentrated until the solid content is 10 wt%, and then stored at 4 DEG C for 1 h, flocculation, filtration, sterilization of the filtrate and drying are performed, and then the composition is obtained.

[0062] Embodiment 2

[0063] The embodiment provides a composition with anti-inflammatory and anti-allergic effects, and raw materials for preparing the composition include Boletus 5 parts, Phyllanthus emblica 20 parts and Rosa roxburghii 10 parts in mass fraction.

[0064] The preparation method comprises the following steps:

[0065] (1) The water content of Boletus is adjusted to 20 wt%, and the Boletus is cut into 2-5 cm particles, and then subjected to steam explosion treatment at 0.6 MPa and 165 DEG C for 40 s, the pressure release rate is less than 0.1 s, the cycle explosion is performed once, the dryness is performed until the water content is 4 wt%, and the crushing is performed until the particle size is not more than 80 meshes, so that a pretreated Boletus product is obtained;

[0066] (2) Adjust the moisture content of Phyllanthus emblica to 35 wt%, and perform steam explosion treatment at 0.5 MPa and 155 ℃ for 100 s with a pressure release rate <0.1 s, and dry to a moisture content of 5 wt%, and crush to not more than 80 meshes to obtain a pretreated Phyllanthus emblica product;

[0067] (3) Directly dry Rosa roxburghii to a moisture content of 5 wt%, and crush to not more than 80 meshes to obtain a pretreated Rosa roxburghii product;

[0068] (4) Mix the pretreated Boletus product, the pretreated Phyllanthus emblica product, the pretreated Rosa roxburghii product and water, and heat to reflux extraction for 0.5 h at a solid-liquid ratio of 1 g:10 mL, extract for 3 times, and pass through 600-mesh filter cloth and a 5-μm filter bag to obtain an extract;

[0069] (5) Concentrate the extract to a solid content of 8 wt%, and store at 2 ℃ for 1.5 h for flocculation, filter, sterilize the filtrate, and dry to obtain the product.

[0070] Example 3

[0071] The present example provides a composition with anti-inflammatory and anti-allergic effects, and the preparation raw materials of the composition include Boletus 20 parts, Phyllanthus emblica 5 parts and Rosa roxburghii 2 parts in terms of mass fraction.

[0072] The preparation method thereof includes:

[0073] (1) Adjust the moisture content of Boletus to 25 wt%, cut to 2-5 cm particles, and perform steam explosion treatment at 0.8 MPa and 180 ℃ for 30 s with a pressure release rate <0.1 s, and dry to a moisture content of 6 wt%, and crush to not more than 80 meshes to obtain a pretreated Boletus product;

[0074] (2) Adjust the moisture content of Phyllanthus emblica to 30 wt%, and perform steam explosion treatment at 0.6 MPa and 165 ℃ for 80 s with a pressure release rate <0.1 s, and dry to a moisture content of 10 wt%, and crush to not more than 80 meshes to obtain a pretreated Phyllanthus emblica product;

[0075] (3) Directly dry Rosa roxburghii to a moisture content of 10 wt%, and crush to not more than 80 meshes to obtain a pretreated Rosa roxburghii product;

[0076] (4) Mix the pretreated Boletus product, the pretreated Phyllanthus emblica product, the pretreated Rosa roxburghii product and water, and heat to reflux extraction for 1 h at a solid-liquid ratio of 1 g:20 mL, extract for 1 time, and pass through 600-mesh filter cloth and a 5-μm filter bag to obtain an extract;

[0077] (5) The extract solution was concentrated to a solid content of 12 wt%, and then flocculated at 6 ℃ for 0.8 h. After filtration, the filtrate was sterilized and dried to obtain the product.

[0078] Example 4

[0079] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (1) of the preparation method is "adjusting the water content of Boletus to 10 wt%, cutting to 2-5 cm particles, steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, pressure release rate <0.1 s, one cycle of explosion, drying to a water content of 4 wt%, and crushing to not more than 80 mesh to obtain a pretreated Boletus product", and other operations remain unchanged.

[0080] Example 5

[0081] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (1) of the preparation method is "adjusting the water content of Boletus to 40 wt%, cutting to 2-5 cm particles, steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, pressure release rate <0.1 s, one cycle of explosion, drying to a water content of 4 wt%, and crushing to not more than 80 mesh to obtain a pretreated Boletus product", and other operations remain unchanged.

[0082] Example 6

[0083] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (1) of the preparation method is "adjusting the water content of Boletus to 20 wt%, cutting to 2-5 cm particles, steam explosion treatment at 0.2 MPa and 130 ℃ for 100 s, pressure release rate <0.1 s, one cycle of explosion, drying to a water content of 4 wt%, and crushing to not more than 80 mesh to obtain a pretreated Boletus product", and other operations remain unchanged.

[0084] Example 7

[0085] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (1) of the preparation method is "adjusting the water content of Boletus to 20 wt%, cutting to 2-5 cm particles, steam explosion treatment at 1.0 MPa and 190 ℃ for 20 s, pressure release rate <0.1 s, one cycle of explosion, drying to a water content of 4 wt%, and crushing to not more than 80 mesh to obtain a pretreated Boletus product", and other operations remain unchanged.

[0086] Example 8

[0087] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (1) of the preparation method is "adjusting the moisture content of Boletus to 20 wt%, cutting to 2-5 cm particles, performing pressure holding treatment at 0.2 MPa, 130 ℃, for 40 s, drying to a moisture content of 4 wt%, and crushing to not more than 80 mesh to obtain a pretreated Boletus product", and other operations remain unchanged.

[0088] Example 9

[0089] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (1) of the preparation method is "drying Boletus to a moisture content of 4 wt%, crushing to not more than 80 mesh to obtain a pretreated Boletus product", and other operations remain unchanged.

[0090] Example 10

[0091] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (2) of the preparation method is "adjusting the moisture content of Phyllanthus emblica to 15 wt%, performing steam explosion treatment at 0.8 MPa, 180 ℃, for 60 s, with a pressure release rate <0.1 s, and cyclic explosion 1 time, drying to a moisture content of 8 wt%, and crushing to not more than 80 mesh to obtain a pretreated Phyllanthus emblica product", and other operations remain unchanged.

[0092] Example 11

[0093] This example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method. The step (2) of the preparation method is "adjusting the moisture content of Phyllanthus emblica to 45 wt%, performing steam explosion treatment at 0.8 MPa, 180 ℃, for 60 s, with a pressure release rate <0.1 s, and cyclic explosion 1 time, drying to a moisture content of 8 wt%, and crushing to not more than 80 mesh to obtain a pretreated Phyllanthus emblica product", and other operations remain unchanged.

[0094] Example 12

[0095] The present example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The step (2) of the preparation method is "adjusting the moisture content of the Phyllanthus emblica to 25 wt%, performing steam explosion treatment at 0.2 MPa, 130°C, for 120 s, with a pressure release rate <0.1 s, with one cycle of explosion, drying to a moisture content of 8 wt%, and pulverizing to not more than 80 mesh to obtain a pretreated Phyllanthus emblica product", and the other operations remain unchanged.

[0096] Example 13

[0097] The present example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The step (2) of the preparation method is "adjusting the moisture content of the Phyllanthus emblica to 25 wt%, performing steam explosion treatment at 1.2 MPa, 200°C, for 30 s, with a pressure release rate <0.1 s, with one cycle of explosion, drying to a moisture content of 8 wt%, and pulverizing to not more than 80 mesh to obtain a pretreated Phyllanthus emblica product", and the other operations remain unchanged.

[0098] Example 14

[0099] The present example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The step (2) of the preparation method is "adjusting the moisture content of the Phyllanthus emblica to 25 wt%, performing pressure holding treatment at 0.2 MPa, 130°C, for 60 s, drying to a moisture content of 8 wt%, and pulverizing to not more than 80 mesh to obtain a pretreated Phyllanthus emblica product", and the other operations remain unchanged.

[0100] Example 15

[0101] The present example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The step (2) of the preparation method is "drying the Phyllanthus emblica to a moisture content of 8 wt%, and pulverizing to not more than 80 mesh to obtain a pretreated Phyllanthus emblica product", and the other operations remain unchanged.

[0102] Example 16

[0103] The present example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The step (5) of the preparation method is "concentrating the extract to a solid content of 10 wt%, sterilizing the filtrate after filtration, and drying to obtain the product", and the other operations remain unchanged.

[0104] Example 17

[0105] The present example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in the preparation method.

[0106] The preparation method is as follows:

[0107] (1) The water content of Boletus edulis is adjusted to 20 wt%, cut into 2-5 cm particles, and subjected to steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate <0.1 s, and the cycle is repeated once, and then dried to a water content of 4 wt%, and ground to not more than 80 mesh, to obtain a pretreated Boletus edulis product;

[0108] (2) The water content of Phyllanthus emblica is adjusted to 25 wt%, and subjected to steam explosion treatment at 0.8 MPa and 180 ℃ for 60 s, with a pressure release rate <0.1 s, and the cycle is repeated once, and then dried to a water content of 8 wt%, and ground to not more than 80 mesh, to obtain a pretreated Phyllanthus emblica product;

[0109] (3) The Rosa roxburghii is dried to a water content of 8 wt%, and ground to not more than 80 mesh, to obtain a pretreated Rosa roxburghii product;

[0110] (4) The pretreated Boletus edulis product, pretreated Phyllanthus emblica product, and pretreated Rosa roxburghii product are mixed with water respectively, with a solid-liquid ratio of 1 g:15 mL, heated to reflux for 45 min, extracted twice, filtered through 600 mesh cloth and 5 μm filter bag, to obtain Boletus edulis extract, Phyllanthus emblica extract, and Rosa roxburghii extract;

[0111] (5) The Boletus edulis extract, Phyllanthus emblica extract, and Rosa roxburghii extract are concentrated to a solid content of 10 wt% respectively, and then stored at 4 ℃ for 1 h for flocculation, and then filtered, and the filtrate is sterilized and dried, to obtain Boletus edulis extract, Phyllanthus emblica extract, and Rosa roxburghii extract;

[0112] (6) The Boletus edulis extract, Phyllanthus emblica extract, and Rosa roxburghii extract are mixed, and the composition is obtained.

[0113] Comparative Example 1

[0114] The present comparative example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in that it does not contain Boletus edulis, and the reduced mass is proportionally distributed to the mass of Phyllanthus emblica and Rosa roxburghii.

[0115] The preparation method is as described in Example 1.

[0116] Comparative Example 2

[0117] The present comparative example provides a composition with anti-inflammatory and anti-allergic effects, which is different from Example 1 only in that it does not contain Phyllanthus emblica, and the reduced mass is proportionally distributed to the mass of Boletus edulis and Rosa roxburghii.

[0118] Preparation method Reference Example 1.

[0119] Comparative Example 3

[0120] This comparative example provides a composition with anti-inflammatory and anti-allergic effects, which is distinguished from Example 1 only in that it does not contain Physalis pruinosa, and the reduced mass is proportionally distributed to the mass of Boletus and Phyllanthus emblica.

[0121] Preparation method Reference Example 1.

[0122] Test Example 1

[0123] Hyaluronidase inhibition effect test

[0124] Prepare 0.25 mg / mL hyaluronidase (HAS) solution and 1 mg / mL sodium hyaluronate solution with 0.1 mM acetic acid buffer. Prepare 1 mg / mL tannic acid and sample solution with water as solvent. Mix 500 μL of HAS solution and 500 μL of sample solution uniformly, set a control blank group, incubate at 37 ℃ for 20 min, add 100 μL of 0.4 M sodium hydroxide solution and 0.4 M potassium borate solution to terminate the reaction, then mix 100 μL of reaction solution with 100 μL of dimethylamino benzaldehyde color developing agent, and measure the absorbance at 37 ℃, 585 nm:

[0125]

[0126] A—Absorbance value of sample solution

[0127] B—Absorbance value of sample blank

[0128] C—Absorbance value of control solution

[0129] D—Absorbance value of control blank

[0130] The value of hyaluronidase inhibition rate (%) is shown in Table 1.

[0131] Table 1

[0132]

[0133] From the data in Table 1, it can be seen that the composition described in the application has excellent hyaluronidase inhibiting effect, and the pretreatment process of Boletus, Phyllanthus emblica and Rosa roxburghii will affect the efficacy of the composition. The composition prepared by mixing extraction of Boletus, Phyllanthus emblica and Rosa roxburghii has certain synergistic effect on the above efficacy, and the lack of any one of them will affect the efficacy of the composition.

[0134] Test Example 2

[0135] Collagenase inhibiting effect test

[0136] Tetracycline hydrochloride (positive control) with a concentration of 1 mg / mL and 2 mg / mL of the sample solution to be tested were prepared with TrisHCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2). Blank group, control group, sample group and sample blank group were set up, 60 L of buffer solution instead of the sample solution to be tested was added to the control group and the sample group, 0.2 mg / mL of collagenase solution was added to the control group and the sample group 140 L of buffer solution instead of the sample solution to be tested was added to the control group and the sample group, 0.2 mg / mL of collagenase solution was added to the control group and the sample group 140 L, the same volume of buffer solution was added instead of the blank group and the sample blank group. After 20 min in a 37 ℃ water bath, 0.5 mg / mL of substrate FALGPA buffer salt solution was added 40 L, 20 min in a 37 ℃ water bath, the absorbance value of each component at 330 nm was measured. The inhibition rate of collagenase was calculated according to the following formula:

[0137] Sample blank group: sample solution 60 μL + buffer 140 μL + substrate 40 μL

[0138] Sample group: sample solution 60 μL + collagenase 140 μL + substrate 40 μL

[0139] Control group: buffer 60 μL + collagenase 140 μL + substrate 40 μL

[0140] Blank group: buffer 200 μL + substrate 40 μL

[0141]

[0142] Each group was measured 3 times on average, the average value was taken, and the collagenase inhibition rate was calculated. The results are shown in Table 2.

[0143] Table 2

[0144]

[0145] From the data in Table 2, it can be seen that the composition described in the application has excellent collagenase inhibitory effect, and the pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition, and the composition prepared by mixing Boletus edulis, Phyllanthus emblica and Rosa roxburghii has certain synergistic effect on the above efficacy, and the absence of any one of them will affect the efficacy of the composition.

[0146] Test Example 3

[0147] Tyrosinase inhibitory effect test

[0148] Prepare 0.5 mg / mL L-tyrosine solution and 100 μg / mL tyrosinase solution with phosphate buffer solution with pH = 6.8 as solvent, and prepare 1 mg / mL arbutin (positive control) solution and sample solution with water as solvent. Mix the tyrosine solution and the tyrosinase solution and the sample solution uniformly, set up a blank control group, and place it in a 37 ℃ environment for 30 min. Measure the absorbance at 475 nm, and calculate the tyrosinase activity inhibition rate of the sample according to the following formula:

[0149]

[0150] Sample group: 100 μL of tyrosine solution + 50 μL of sample solution + 50 μL of tyrosinase solution

[0151] Sample blank group: 100 μL of tyrosine solution + 50 μL of sample solution + 50 μL of buffer

[0152] Control group: 100 μL of tyrosine solution + 50 μL of tyrosinase + 50 μL of buffer solution

[0153] Control blank group: 100 μL of tyrosine solution + 100 μL of buffer solution

[0154] Each group is measured for an average of 3 times, and the average value is taken to calculate the tyrosinase inhibition rate value. The results are shown in Table 3.

[0155] Table 3

[0156]

[0157] From the data in Table 3, it can be seen that the composition described in the application has excellent tyrosinase inhibitory effect, and the pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition, and the composition prepared by mixing Boletus edulis, Phyllanthus emblica and Rosa roxburghii has certain synergistic effect on the above efficacy, and the absence of any one of them will affect the efficacy of the composition.

[0158] Test Example 4

[0159] α-amylase inhibitory effect test

[0160] Take 250 μL of phosphate buffer (pH 6.8), add 50 μL of the test sample (1 mg / mL) and 50 μL of α-amylase solution (1 unit / mL), shake well, mix at 37 °C and incubate in a water bath for 10 min. Add 200 μL of 1% soluble starch solution, mix at 37 °C and incubate in a water bath for another 10 min. Add 200 μL of 3,5-dinitrosalicylic acid (DNS reagent), mix well, incubate in a water bath at 80 °C for 10 min, then rapidly cool. Observe until the control group shows obvious color change, then stop. Use phosphate buffer instead of enzyme solution as a blank control and acarbose as a positive control. Each experiment is repeated 3 times. Measure the absorbance at 540 nm, and take the average absorbance of each sample from multiple wells to calculate the α-amylase inhibition rate of each test sample.

[0161]

[0162] Each group was measured 3 times on average, and the average value was taken. The results of the α-amylase inhibition rate (%) are shown in Table 4.

[0163] Table 4

[0164]

[0165] As shown in Table 4, the composition described in this application has excellent α-amylase inhibition effect. The pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition. The composition prepared by mixed extraction of Boletus edulis, Phyllanthus emblica and Prickly pear has a certain synergistic effect on the above-mentioned efficacy. The absence of any one of them will affect the efficacy of the composition.

[0166] Test Example 5

[0167] Anti-inflammatory effect test

[0168] RAW264.7 cells in logarithmic growth phase with good morphology were selected and prepared into 5×10⁶ cells using complete culture medium. 4 Cell suspension of cells / mL, at a ratio of 1000 cells / well. L was inoculated in 24-well plates, i.e., 5 × 10⁻⁶ wells. 4 Each sample was incubated at 37 °C in a 5% CO2 incubator for 24 h.

[0169] Drug administration and LPS: Blank control group, model control group, positive control group and sample group were set up.

[0170] Blank control group: Add complete culture medium to each well.

[0171] Positive control group: Add 5g of completely diluted culture medium to each well. M dexamethasone acetate sodium (DEX)

[0172] Negative control group: final concentration of 1 g / mL of LPS

[0173] Sample group: add the sample to be tested (concentration of 250 g / mL) diluted with complete medium to each well

[0174] Set 3 duplicate wells for each group, incubate at 37°C in a 5% CO2 incubator for 2 h, add 1 g / mL of LPS to each well except the blank control group, and incubate in the incubator for another 24 h.

[0175] Inflammatory factor detection: detect the content of NO in the collected cell supernatant according to the steps of the NO detection kit instructions. Analyze the experimental results by data processing software, and the results are shown in Table 5.

[0176] Table 5

[0177]

[0178] Note: ### indicates the comparison with the blank control group, p <0.001; *** indicates the comparison with the model control group, p <0.001.

[0179] As shown in Table 5, LPS stimulation led to a significant increase in the relative expression of NO ( p <0.001), indicating that the inflammation model was successfully constructed. Compared with the model control group, the test sample treatment significantly reduced the relative expression of NO ( p <0.05), and the effects of Examples 1-3 were at a higher level. The above results show that the composition of Boletus, Phyllanthus emblica and Rosa roxburghii has strong anti-inflammatory effect, and has certain synergistic effect, and the absence of any one of them will affect the efficacy of the composition.

[0180] Test Example 6

[0181] Anti-allergy test

[0182] Randomly select 3 dpf wild type AB strain zebrafish in 24-well plates, and treat 10 zebrafish in each well. The concentration of the test sample is 250 μg / mL, the positive control cromolyn sodium is 1000 μg / mL, and the normal control group and the model control group are set up at the same time, and the volume of each well is 1 mL, and the experiment is carried out in triplicate. Except for the normal control group, the rest of each experimental group is water-soluble to give compound C48 / 80 to establish a zebrafish allergic model. After co-treatment of each experimental group with reagent BAPNA for 24 h, the liquid is transferred to a 96-well plate, 10 tails / 250 μL / well, and the OD 405 value of the expression level of tryptase in each experimental group is detected

[0183]

[0184] Sample group: average value of absorbance of each sample group

[0185] Normal control group: average value of absorbance of the normal control group without giving compound C48 / 80

[0186] Sample blank group: absorbance value of the same volume of sample blank

[0187] Normal blank group: absorbance value of the same volume of embryo culture solution

[0188] The results are shown in the following table. Figure 1

[0189] As can be seen from the above, the composition described in the present application has excellent anti-allergic effect, and can significantly reduce the expression amount of tryptase, and the effect is better than that of boletus extract, phyllanthus extract and roxburgh rose extract. Figure 1 The applicant declares that the present application illustrates a composition with anti-inflammatory and anti-allergic effect and its application through the above examples, but the present application is not limited to the above examples, that is, it does not mean that the present application must rely on the above examples to be implemented. Those skilled in the art should understand that any improvement of the present application, equivalent replacement of each raw material of the product of the present application, addition of auxiliary ingredients, selection of specific modes, etc. all fall within the protection scope and disclosure scope of the present application.

[0190] The preferred embodiments of the present application are described in detail above, but the present application is not limited to the specific details in the above embodiments, and within the technical concept range of the present application, various simple modifications can be made to the technical solutions of the present application, and these simple modifications all belong to the protection scope of the present application.

[0191]

[0192] ​​It should be further noted that various specific technical features described in the above specific embodiments can be combined in any suitable manner, and in order to avoid unnecessary repetition, the present application will not describe various possible combinations again.

Claims

1. A composition having anti-inflammatory and anti-allergic effects, characterized in that, The raw materials for preparing the composition include, by mass parts, 5-20 parts of Boletus edulis, 5-20 parts of Phyllanthus emblica, and 2-10 parts of Prickly pear. The composition is prepared by a method comprising the following steps: (1) Boletus edulis and Phyllanthus emblica were pretreated by steam explosion. (2) Mix the pretreated Boletus, the pretreated Phyllanthus emblica, and Prickly pear with water, and reflux to extract the extract. (3) Filter and concentrate the extract to obtain a concentrated solution. Refrigerate the concentrated solution to flocculate it, filter it, and then dry it to obtain the final product. The water content of the porcini mushrooms mentioned in step (1) is 20-30 wt%, and the water content of the amla is 25-35 wt%. The steam explosion of the porcini mushrooms is carried out at a pressure of 0.4-0.8 MPa, a temperature of 140-180 ℃, and a time of 30-60 s. The pressure of the steam explosion of Phyllanthus emblica is 0.5-0.8 MPa, the temperature is 150-180 ℃, and the time is 60-100 s.

2. The composition with anti-inflammatory and anti-allergic effects according to claim 1, characterized in that, The reflux extraction time is 0.5-1 h, and the number of times is 1-3.

3. The composition with anti-inflammatory and anti-allergic effects according to claim 1, characterized in that, The material-to-liquid ratio for the reflux extraction is 1 g:(10-20) mL.

4. The composition with anti-inflammatory and anti-allergic effects according to claim 1, characterized in that, The refrigerated flocculation temperature is 2-6 ℃, and the time is 0.5-2 h.

5. The use of a composition having anti-inflammatory and anti-allergic effects according to any one of claims 1-4 in the preparation of cosmetics having anti-inflammatory and / or anti-allergic effects.

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