Ecological improvement matrix prepared from waste slag stone and capable of improving stability of soil aggregate and preparation method of ecological improvement matrix

Abstract

The invention belongs to the technical field of solid waste resource utilization, and discloses an ecological improvement matrix prepared from waste slag stone and used for improving soil aggregate stability and a preparation method of the ecological improvement matrix. According to the method, the problem of resource utilization of the waste slag stone in the production and processing process of a stone factory is solved, the waste particle slag stone is crushed, and biomass charcoal, corn straw, urea and a microbial agent which has the capacity of dissolving phosphorus, dissolving potassium and fixing nitrogen and can generate exopolysaccharides, such as paenibacillus mucilaginosus JZ1 and scenedesmus EMC7 are added, so that the biomass charcoal, the corn straw, the urea and the microbial agent are added, and the biomass charcoal, the corn straw and the urea are added. And fully stirring to prepare the ecological improvement substrate. The ecological improvement matrix is applied to soil, soil nutrients can be released in a long-acting mode, the content of large aggregates in the soil is increased through electronic adsorption, organic and inorganic cementation and other modes, the damage rate of the soil aggregates is reduced, the corrosion resistance of the soil is improved, and finally reasonable utilization of slag stone resources is achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of solid waste resource utilization, and particularly relates to an ecological improvement substrate for improving the stability of soil aggregates based on waste slag stone of a stone processing plant and a preparation method thereof. BACKGROUND

[0002] Soil erosion is one of the major environmental problems faced globally, it not only leads to land degradation, but also seriously affects agricultural production and the health of the ecosystem. The occurrence of soil erosion is related to a variety of factors, including water flow erosion, wind action, land use patterns, and the nature of the soil itself. Among the many influencing factors, the stability of soil aggregates is a key parameter that determines the ability of soil to resist erosion. Soil aggregates are porous structures formed by the cementation of soil particles through organic and inorganic substances, their size, shape and stability directly affect the porosity, air permeability and water retention of the soil, and thus affect the growth of plant roots and the cycling of soil nutrients.

[0003] Currently, conventional soil improvement methods have limitations and are difficult to fully meet the dual needs of improving aggregate stability and waste resource utilization. Slag stone, as an industrial byproduct, is usually derived from waste generated during mining, road construction, building demolition or industrial production. These materials are often considered waste, and waste slag stones (such as crushed stone, stone powder) occupy land and are prone to dust pollution, but their potential value is rarely tapped. Slag stone is mainly composed of minerals, rich in minerals such as silicon and calcium, and has a certain particle size and hardness, which makes it have potential application value in improving soil structure. The physical properties of slag stone, such as particle size distribution and surface roughness, can increase the porosity of the soil, improve the aeration and permeability of the soil, and adsorb organic matter, thereby helping to enhance the stability of soil aggregates. Microorganisms play a core role in the material cycle of the soil ecosystem, and certain microbial groups also play a crucial role in the formation and stability of soil structure. They secrete polysaccharides, proteins, glycoproteins and other sticky extracellular polymers during growth and metabolism, which act as natural "cementing agents" to bind soil particles together, promoting the formation of soil aggregates. The functioning of the above-mentioned functions of microorganisms is based on the ability of microorganisms to survive and reproduce in the environment. Biochar and corn straw are rich in organic matter, providing a good environment for the healthy growth of microorganisms and further improving the activity of microorganisms, promoting the role of microorganisms in material conversion and soil structure optimization in the soil.

[0004] The prior art, such as preparation of substrates using coal gangue and sludge, steel slag-coal gangue composite substrates, etc., although realizes the resource utilization of solid waste, but the targeted improvement of soil aggregate stability is insufficient. Soil aggregate is the key carrier of soil organic carbon stability, and its mineral-organic complex-microorganism characteristics directly affect the water and fertilizer retention capacity of soil. Therefore, by processing waste slag stone in a stone mill, combined with agricultural and forestry waste, specific functional microorganisms, etc., an ecological restoration substrate material is prepared, which not only realizes the recycling of waste slag stone resources, improves the ecological restoration effect, but also eliminates air, water pollution and soil erosion caused by random piling of waste. SUMMARY

[0005] The purpose of the present application is to provide an ecological improvement substrate based on waste slag stone configuration for improving soil aggregate stability and a preparation method thereof, realizing the recycling of waste slag stone resources.

[0006] The present application provides an ecological improvement substrate based on slag stone, supplemented by the addition of ecological carbon, straw, specific functional microorganisms and urea, to form an ecological improvement substrate. The substrate can effectively improve the stability of soil aggregates and the resistance of soil through organic and inorganic cementation. In addition, the added functional microbial strains can not only increase the soil exopolysaccharide, but also effectively release trace elements and nutrients in the substrate, which is beneficial to the improvement of soil quality.

[0007] Specifically, the present application provides an ecological improvement substrate based on waste slag stone configuration for improving soil aggregate stability, which is based on slag stone, supplemented by the addition of biomass carbon, plant straw, specific functional microorganisms and urea, to form an ecological improvement substrate. The specific functional microorganism is a microorganism with the functions of phosphorus and potassium solubilization, nitrogen fixation, and the ability to produce exopolysaccharide.

[0008] In the ecological improvement substrate, the volume ratio of slag stone, biomass carbon, straw, urea and specific functional microorganism is: 0-8.0:0.5-2.0:0.5-2.0:0.025-0.1:0.05-0.2; preferably 0-4.0:0.5-2.0:0.5-2.0:0.025-0.1:0.05-0.2; more preferably 0-2.0:0.5-2.0:0.5-2.0:0.025-0.1:0.05-0.2; more preferably 0-1.0:1:1:0.05:0.1; more preferably 0.5:1:1:0.05:0.1; further preferably 1.0:1:1:0.05:0.1.

[0009] The microorganism is Paenibacillus mucilaginosus and / or Scenedesmus sp., preferably, the microorganism is Paenibacillus mucilaginosus JZ1, with a preservation number of CGMCC No.28359, and / or Scenedesmus sp. EMC7, with a preservation number of CGMCC No.41200.

[0010] In the ecological improvement substrate, the concentration of JZ1 is 10 10 CFU / mL, and the concentration of EMC7 is 10 6 CFU / mL, and the two are mixed at a volume ratio of 1:1.

[0011] The ecological improvement substrate is prepared by mixing the slag, biomass charcoal, straw, urea and microorganism according to the proportion of claim 2.

[0012] The application also provides an ecological improvement product comprising the ecological improvement substrate.

[0013] The application also provides a preparation method of the ecological improvement substrate for improving the stability of soil aggregates by using waste slag, which comprises mixing slag, biomass charcoal, straw, urea and specific functional microorganism according to the above-mentioned proportion, wherein the specific functional microorganism is a microorganism with the functions of phosphorus solubilization, potassium solubilization, nitrogen fixation and extracellular polysaccharide production, preferably, the microorganism is Paenibacillus mucilaginosus and / or Scenedesmus sp., more preferably, the microorganism is Paenibacillus mucilaginosus JZ1, with a preservation number of CGMCC No.28359, and / or Scenedesmus sp. EMC7, with a preservation number of CGMCC No.41200.

[0014] The application also provides a method for recycling waste slag resources, which comprises mixing the waste slag, biomass charcoal, straw, urea and specific functional microorganism according to the above-mentioned proportion to prepare an ecological improvement substrate, and further preferably, adding the ecological improvement substrate to soil, preferably, the microorganism is Paenibacillus mucilaginosus and / or Scenedesmus sp., more preferably, the microorganism is Paenibacillus mucilaginosus JZ1, with a preservation number of CGMCC No.28359, and / or Scenedesmus sp. EMC7, with a preservation number of CGMCC No.41200.

[0015] The application also provides the use of the ecological improvement substrate for improving soil.

[0016] The application also provides a method for improving soil, wherein the ecological improvement substrate is added to the soil.

[0017] The straw can be corn straw, sorghum straw, wheat straw, cotton straw or other plant straw, which can provide a good environment for the healthy growth of microorganisms, further improve the activity of microorganisms, and promote the microorganisms to play the role of material conversion and soil structure optimization in the soil.

[0018] The ecological improvement substrate of the application is applied to the soil, can long-acting release soil nutrients, increase the content of large aggregates in the soil through electron adsorption, organic and inorganic cementation, reduce the damage rate of soil aggregates, improve the corrosion resistance of the soil, and finally realize the rational utilization of slag resources. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 Quantitative determination of exopolysaccharide produced by three strains of algae.

[0020] Figure 2 Soil physical property characteristics of different addition level sample plots.

[0021] Figure 3 Soil aggregate water stability mass fraction of different treatment levels.

[0022] Figure 4 Soil aggregate mean weight diameter of different treatment levels.

[0023] Figure 5 Soil aggregate geometric mean diameter of different treatment levels.

[0024] Figure 6 Erosion sediment content change characteristics over time of different treatment levels.

[0025] wherein, Figures 3 to 6 In the formula, treatment 1, treatment 2 and treatment 3 represent that the soil of the sample plot is added with substrate 1, substrate 2 and substrate 3 respectively, and the control is soil without adding the substrate. Different lowercase letters represent that there is a significant difference between different treatments in the same land use type, P<0.05.

[0026] Biological material preservation information:

[0027] The Paenibacillus mucilaginosus JZ1 is a bacterial strain, which was preserved in the China General Microbiological Culture Collection Center on September 5, 2023, and the abbreviation thereof is CGMCC (unit address: No. 3, Beichen West Road, Chaoyang District, Beijing), the preservation number thereof is CGMCC No. 28359, and the classification and naming thereof is Paenibacillus mucilaginosus.

[0028] Scenedesmus sp. strain EMC7 was deposited in China General Microbiological Culture Collection Center on July 5, 2024, and the deposit number is CGMCC No. 41200, and the classification name is Scenedesmus sp. DETAILED DESCRIPTION

[0029] The technical solutions of the present application are further described below by examples.

[0030] I. Raw materials of ecological substrate and preparation

[0031] The raw materials include waste slag (from Zhongtie No. 4 Bureau slag sand and stone processing yard), biomass charcoal (made by Beijing Jingpu Garden Biological Engineering Co., Ltd. by pyrolysis and carbonization of landscaping waste), corn straw collected from local farmland in Jian Shui, Yunnan, and urea purchased from Ma Junwei Agricultural Operating Department in Jian Shui County, Yunnan (produced by Anyang Zhongying Fertilizer Co., Ltd.).

[0032] The obtained waste slag is mechanically granulated by using a vibrating screen machine and a pulverizer, and the particle size is controlled to be less than 0.053 mm. The obtained biomass charcoal is mechanically pulverized to make the particle size less than 0.125 mm. The corn straw is dried to make the water content less than 10%, and then pulverized by using a pulverizer to ensure that the diameter of the straw is less than 5 mm.

[0033] II. Screening of microbial and algal strains

[0034] To promote soil nutrient transformation and improve soil aggregates, 5 strains of bacteria and 3 species of algae with the functions of phosphorus and potassium dissolution, nitrogen fixation, and the ability to produce extracellular polysaccharides were selected from the Karst rocky desertification area in Jian Shui, Yunnan, which were isolated in the laboratory earlier. The selected microbial strains were determined by quantitative determination.

[0035] Among them, JZ1 is a bacterial strain with a preservation number of CGMCC No. 28359. The preservation number of EMC7 algal strain is CGMCC No. 41200.

[0036] The quantitative determination method of phosphorus dissolution capacity is as follows: 5 strains of bacteria are inoculated in inorganic phosphorus liquid medium (medium formula: glucose 10 g / L, FeSO4·7H2O 0.03 g / L, NaCl 0.3 g / L, KCl 0.3 g / L, MnSO4·4H2O 0.03 g / L, MgSO4·7H2O 0.3 g / L, (NH4)2SO4 0.5 g / L, CaPO4 10 g / L, natural pH, 121 °C sterilization for 30 min) with an inoculation amount of 1%, and cultured at 30 °C and 180 r·min-1 After 72 h of shaking culture, the bacterial suspension was centrifuged at 10,000 r / min for 15 min at 4 ℃, 2 ml of supernatant was taken and reserved, and sterile culture medium was used as a blank reference. 1 ml of sample was transferred to a 25 ml volumetric flask, diluted with water to about 15 ml, 2-3 drops of nitrophenol indicator were added, and the solution was adjusted to light yellow with 4 mol / L NaOH solution, 2.5 ml of molybdenum-antimony color developing agent was accurately added, shaken well, and water was added to constant volume, and placed at room temperature above 15 ℃ for 30 min. The absorbance of the solution was determined by colorimetry at a wavelength of 700 nm, and the corresponding phosphorus content (μg / ml) was calculated.

[0037] The quantitative determination method of potassium-lysing ability is as follows: 5 strains of bacteria are inoculated in 100 mL conical flasks containing 30 mL of potassium-lysing liquid medium at an inoculation amount of 1%, and cultured in a 28 ℃, 180 rpm shaking bed for 7 days. An equal volume of potassium-lysing liquid medium without inoculation of bacterial strains is used as a blank control. The fermentation broth after 7 days is collected, and 30% H2O2 is used for digestion until it is clear and transparent, about 30-60 min, centrifuged at 6000 rpm for 10 min, and the supernatant is transferred to a 50 mL volumetric flask for constant volume. The content of soluble potassium is determined using a flame spectrophotometer.

[0038] The quantitative determination method of nitrogen fixation ability is as follows: 5 strains of bacteria are inoculated in 100 mL conical flasks containing 30 mL Ashby nitrogen-free liquid medium at an inoculation amount of 1%, and cultured at 30°C, 180 rpm on a shaking table for 24 h, centrifuged at 6000 r / min for 10 min, and the supernatant is filtered through a 0.25 μm filter membrane to remove bacteria and stored for use. The nitrogen fixation ability of microorganisms is evaluated by the activity of nitrogenase. The determination method of nitrogenase activity is analyzed by using nitrogenase (NITS) enzyme-linked immunoassay kit (Jiangsu Meijiang Biological Technology Co., Ltd., JM-1305702-48T). Blank holes (blank control holes do not add samples and enzyme-labeled reagents, and the rest of the steps are the same), standard holes and sample holes are set. The specific operation is as follows: accurately add 50 μL of standard sample to the enzyme-labeled coating plate, add 40 μL of sample diluent to the sample hole first, then add 10 μL of sample to be tested (the final dilution of the sample is 5 times) and shake gently to mix, seal the plate with sealing film after incubation at 37°C for 30 min, and then pour off the diluted supernatant; dilute the reagent kit with washing solution 30 times, and add it to each sample hole, stand for 30 s, then discard, repeat 5 times, and pat dry; except for the blank control hole, 50 μL of enzyme-labeled reagent is added to each hole, and incubated at 37°C for 30 min again; repeat the above washing steps; then add 50 μL of color developing agent A and 50 μL of color developing agent B to each hole in turn, shake gently to mix, and develop color at 37°C for 10 min; add 50 μL of stop solution to each hole to stop the reaction; adjust 0 with the blank hole, and measure the absorbance (OD value) of each hole at 450 nm wavelength with an enzyme-labeled instrument. According to the final concentration of the standard material liquid, the nitrogenase activity of each strain supernatant is calculated.

[0039] Quantitative determination of exopolysaccharide (EPS): 5 strains of bacteria were inoculated in 10% TSB liquid medium at an inoculation amount of 1%, and cultured at 30°C, 180 rpm for 48 h; 3 strains of algae were inoculated in 30 mL of BG11 liquid medium at an inoculation amount of 5%, and cultured at 27°C, 2700 lx light, with shaking for 2 minutes at a fixed time every day, for 4 weeks. The fermentation broth was centrifuged at 4000 rpm for 15 min, and the supernatant was added with 3 times the volume of anhydrous ethanol, and placed in a refrigerator at 4°C overnight, then centrifuged at 4000 rpm for 15 min to obtain the precipitate, which was dissolved with an appropriate amount of distilled water to obtain an EPS sample solution. The polysaccharide content was determined by anthrone sulfuric acid colorimetry. A glucose standard solution (0.1 g / L) was prepared, and a number of test tubes with stoppers were taken, and different proportions of the glucose standard solution and the prepared anthrone sulfuric acid solution were added to the test tubes according to the table. After adding the reagents, the test tubes were shaken well, placed in a boiling water bath for 15 min, and then taken out and cooled to room temperature. The absorbance of each tube at 620 nm was measured by an enzyme-labeled instrument, and a standard curve was drawn according to the measured absorbance. 1 mL of the sample was taken, and the absorbance at 620 nm was measured according to the same method, and the exopolysaccharide content was calculated according to the standard curve.

[0040] Table 1, Quantitative determination results of the functional effects of 5 strains of bacteria

[0041]

[0042] The higher the phosphorus solubilization, potassium solubilization, and nitrogen fixation are, the stronger the ability to convert and fix nutrients in the soil is, and the better the improvement of soil nutrients is. The higher the exopolysaccharide content is, the greater the contribution of the microorganism to the stability of soil aggregates is. As shown in Table 1, JZ1 has the abilities of phosphorus solubilization, potassium solubilization, and nitrogen fixation, and can also produce exopolysaccharide, so JZ1 is selected as the bacterial strain in the subsequent preparation of the ecological substrate. Figure 1 It can be seen that the exopolysaccharide content in the fermentation broth of the algae strain EMC7 is high, and in addition, it is verified that there is no antagonism between JZ1 and EMC7, so the algae strain is selected as the algae strain in the subsequent preparation of the ecological substrate, and the two microorganisms can be used together to prepare the ecological substrate.

[0043] III. Fermentation of JZ1 bacteria and EMC7 algae and preparation of ecological substrate

[0044] The JZ1 strain was inoculated into 10% TSB liquid medium at an inoculation amount of 1%, and cultured on a shaking table at 30°C for 36 h, then the absorbance of the bacterial solution was measured, the number of microbial cells was calculated, and the number of cells was adjusted to 10 10CFU / mL. The EMC7 strain was inoculated into BG11 liquid medium at an inoculation amount of 5%, and cultured at 28°C under illumination of 2700 lx, with fixed-time shaking twice a day for 1 minute each time. After 4 weeks of culture, the OD680 value was measured, and the number of algal cells was adjusted to 10 6 CFU / mL.

[0045] The diluted JZ1 and EMC7 were mixed at a volume ratio of 1:1, and each material prepared in the first part was formulated at different proportions, with the number of surviving microorganisms after addition as the main evaluation basis. The specific raw material ratio settings are shown in Table 2.

[0046] After the materials were thoroughly mixed, the mixed microbial liquid was uniformly sprayed on the mixture according to a solid-liquid ratio of 1:0.1 (V / V), and further stirred uniformly, and placed at room temperature in the dark. The number of surviving JZ1 and EMC7 was measured after 1 week and 2 months of storage.

[0047] Table 2, different raw material addition ratios (by volume ratio)

[0048]

[0049] As can be seen from Table 3, the number of surviving JZ1 and EMC7 decreased with the extension of storage time. Among them, in the raw material ratios of treatment 1, treatment 2 and treatment 3, the number of viable bacteria of JZ1 could be maintained at 10 8 CFU / g, and the number of viable bacteria of EMC7 could also be maintained at 10 4 CFU / g after 1 week and 2 months of storage. Therefore, the raw material ratios of treatment 1, treatment 2 and treatment 3 were selected, and two kinds of microorganisms were added, and the effect of the two kinds of microorganisms on soil aggregates was further studied.

[0050] Table 3, effect of different raw material addition ratios on the number of surviving JZ1 and EMC7

[0051]

[0052] Four, the effect of ecological substrates prepared based on different proportions of waste slag on soil aggregates of different land use types

[0053] As shown in Table 4, the slag stone (<0.053 mm), biomass charcoal, corn straw, urea and microbial fermentation liquid were mixed according to different volume ratios to form three types of substrates. The forest soil without adding the substrate was used as a control group. The three types of substrates (substrate 1, substrate 2 and substrate 3) were added to the Thuja sutchuenensis and Pinus massoniana forest soil (calcareous red soil, Jiubiao State-owned Forest Farm in Yunnan Jian Shui) respectively according to the specification of 150 g / kg, and were ploughed to a depth of 0-10 cm. The sample plot was set to 1x1 m to form three groups of treatment 1, treatment 2 and treatment 3. Each treatment level was set to four replicates to ensure that the substrate was fully mixed with the soil. After 8 months, the soil samples were collected and the soil physical indicators were determined, including the mass fraction of aggregate, mean weight diameter (MWD) and geometric mean diameter (GMD).

[0054] Table 4, raw material table of ecological improvement substrate

[0055]

[0056] The soil bulk density, porosity and water content characterize the aeration and water permeability of the soil. As shown in Table 5, in the Thuja sutchuenensis forest, the soil bulk density of the treatment 2 level sample plot was significantly lower than that of the other sample plots (P<0.05), which was about 0.86 times that of the control sample plot. Figure 2

[0057] In addition, the total porosity (53%), capillary water holding capacity (31%) and saturated water content (51%) of the treatment 2 level sample plot were the highest, followed by the treatment 3, treatment 1 and control sample plots. In the Pinus massoniana forest, the capillary water holding capacity and saturated water content of the treatment 2 level sample plot were significantly higher than those of the treatment 3, treatment 1 and control sample plots. In terms of total porosity, the porosity of the treatment 2 sample plot was higher than that of the treatment 3 sample plot, but there was no significant difference between them (P>0.05). The soil bulk density of the control and treatment 1 sample plots was significantly higher than that of the treatment 3 and treatment 2 sample plots (P<0.05).

[0058] As shown in Table 6, in the Thuja sutchuenensis forest, the content of large aggregates (>0.25 mm) in the treatment 2 was the highest (91.3%), followed by the treatment 3 (85.89%), treatment 1 (83.04%) and control sample plot (72.22%). In the Pinus massoniana forest, the content of large aggregates (>0.25 mm) in the treatment 2 was the highest (89.5%), followed by the treatment 1 (85.74%), treatment 3 (81.83%) and control sample plot (58.84%). Comprehensive comparison found that the mass fraction of large aggregates in the soil added with the substrate was significantly higher than that in the soil without adding the substrate. Figure 3

[0059] As shown in Table 6, in the Thuja sutchuenensis forest, the content of large aggregates (>0.25 mm) in the treatment 2 was the highest (91.3%), followed by the treatment 3 (85.89%), treatment 1 (83.04%) and control sample plot (72.22%). In the Pinus massoniana forest, the content of large aggregates (>0.25 mm) in the treatment 2 was the highest (89.5%), followed by the treatment 1 (85.74%), treatment 3 (81.83%) and control sample plot (58.84%). Comprehensive comparison found that the mass fraction of large aggregates in the soil added with the substrate was significantly higher than that in the soil without adding the substrate.

[0059] As shown in Table 6, in the Thuja sutchuenensis forest, the content of large aggregates (>0.25 mm) in the treatment 2 was the highest (91.3%), followed by the treatment 3 (85.89%), treatment 1 (83.04%) and control sample plot (72.22%). In the Pinus massoniana forest, the content of large aggregates (>0.25 mm) in the treatment 2 was the highest (89.5%), followed by the treatment 1 (85.74%), treatment 3 (81.83%) and control sample plot (58.84%). Comprehensive comparison found that the mass fraction of large aggregates in the soil added with the substrate was significantly higher than that in the soil without adding the substrate. Figure 4and Figure 5 As shown in the table, in the Thuja stand, the mean weight diameter (MWD) and geometric mean diameter (GMD) of the soil aggregates were 3.04-3.69 mm and 0.95-2.09 mm, respectively. The MWD (3.69 mm) and GMD (2.09 mm) of the soil aggregates in the treatment 2 plot were the largest, followed by the treatment 1 plot (MWD: 3.34 mm, GMD: 1.44 mm), the treatment 3 plot (MWD: 3.14 mm, GMD: 1.46 mm), and the control plot (MWD: 3.04 mm, GMD: 0.95 mm). In the Pinus massoniana stand, the MWD and GMD of the soil aggregates were 2.35-4.16 mm and 0.5-2.35 mm, respectively. The MWD (4.16 mm) and GMD (2.35 mm) of the soil aggregates in the treatment 2 plot were the largest, followed by the treatment 1 plot, the treatment 3 plot, and the control plot.

[0060] V. Effects of artificial ecological substrates based on waste slag on soil erosion

[0061] The soil (calcareous red soil) was filled in the soil flume (1 m long, 0.3 m wide, and 45 cm deep) to a soil layer thickness of 40 cm, and the slope was set to 10°. To ensure good water permeability of the soil flume during the simulated rainfall process, the bottom of the soil flume was uniformly perforated, and 5 cm of fine sand was placed at the bottom. To ensure the uniformity of the filled soil, the soil was filled in layers, with each layer being 5 cm thick.

[0062] Before filling the upper layer of soil, the surface of the lower layer of soil was grabbed to reduce soil layering. In the surface soil layer (0-10 cm), three types of substrates (see Table 4 for the ratio) were added according to the specification of 150 kg / acre. The surface soil (0-10 cm) was mixed with the substrates, and a soil flume without added substrates was set as a control. A 2 mm aperture screen was placed above the surface soil, and the surface soil was pre-wetted under the condition of a rainfall intensity of 45 mm / h until water flow began to occur, and then the rainfall was stopped. After being placed outdoors for 8 months, a rainfall experiment was conducted with a rainfall intensity of 45 mm / h. Once runoff occurred, plastic buckets were used to collect runoff samples every 5 min. The rainfall time was 50 min after runoff occurred to ensure that the surface runoff reached a stable rate. The concentration of runoff sediment was determined based on the collected samples.

[0063] As Figure 6As shown, the soil added with artificial ecological substrate (treatment 1-3) has lower runoff sediment content than the soil without artificial ecological substrate (control) during rainfall erosion, and treatment 2 and treatment 3 are higher than treatment 1 in terms of erosion resistance.

[0064] Therefore, the ecological substrate prepared in the application has important roles in improving soil physical properties, improving soil aggregate stability and soil erosion resistance, etc.

Claims

1. An ecological improvement substrate for enhancing soil aggregate stability using waste slag, characterized in that, An ecological improvement substrate is made by using slag as a base and supplementing it with biochar, plant straw, specific functional microorganisms and urea. The functional microorganisms are microorganisms that have the functions of phosphorus solubilization, potassium solubilization and nitrogen fixation, and can produce extracellular polysaccharides.

2. The ecological improvement substrate according to claim 1, characterized in that, The volume ratio of the slag, biochar, straw, urea, and functional microorganisms is: 0-8.0:0.5-2.0:0.5-2.0:0.025-0.1:0.05-0.2; preferably 0-4.0:0.5-2.0:0.5-2.0:0.025-0.1:0.05-0.2; even more preferably 0-2.0:0.5-2.0:0.5-2.0:0.025-0.1:0.05-0.2; more preferably 0-1.0:1:1:0.05:0.1; even more preferably 0.5:1:1:0.05:0.1; and even more preferably 1.0:1:1:0.05:0.

1.

3. The ecological improvement substrate according to claim 2, characterized in that, The functional microorganism is Paenibacillus mucilaginosus and / or Scenedesmus sp., preferably Paenibacillus mucilaginosus JZ1, with accession number CGMCC No. 28359, and / or Scenedesmus sp. EMC7, with accession number CGMCC No. 41200.

4. The ecological improvement substrate according to claim 3, characterized in that, The concentration of JZ1 is 10. 10 CFU / mL, EMC7 concentration is 10 6 CFU / mL, the two are mixed at a volume ratio of 1:

1.

5. The ecological improvement substrate according to any one of claims 1-4, characterized in that, It is made by mixing the slag, biochar, straw, urea and microorganisms in a certain proportion.

6. An eco-friendly improved product, characterized in that, It includes the ecological improvement substrate as described in any one of claims 1-5.

7. A method for preparing an ecological improvement substrate as described in any one of claims 1 to 5, characterized in that, It is made by mixing slag, biochar, straw, urea and specific functional microorganisms in a certain proportion. The specific functional microorganisms are microorganisms with phosphorus solubilization, potassium solubilization and nitrogen fixation functions, and can produce extracellular polysaccharides. Preferably, the microorganisms are Paenibacillus mucilaginosus and / or Scenedesmus sp. More preferably, the microorganisms are Paenibacillus mucilaginosus JZ1, with accession number CGMCC No. 28359, and / or Scenedesmus EMC7, with accession number CGMCC No. 41200.

8. A method for reusing waste slag resources, characterized in that, The method includes mixing the waste slag, biochar, straw, urea, and functional microorganisms in the proportions described in claim 2 to prepare an ecological improvement substrate. More preferably, the method includes adding the ecological improvement substrate to the soil. Preferably, the microorganisms are Paenibacillus mucilaginosus and / or Scenedesmus sp.; more preferably, the microorganisms are Paenibacillus mucilaginosus JZ1, with accession number CGMCC No. 28359, and / or Scenedesmus sp. EMC7, with accession number CGMCC No. 41200.

9. The use of the ecological improvement substrate according to claims 1-5 for soil improvement.

10. A method for improving soil, characterized in that, Add the ecological improvement substrate described in claims 1-6 to the soil.

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