Infant formula milk powder with low beta-lactoglobulin and preparation method thereof

Abstract

The invention relates to the technical field of formula milk powder, in particular to infant formula milk powder with low beta-lactoglobulin and a preparation method of the infant formula milk powder. In order to enable the milk powder to be closer to breast milk components, the components in the milk powder are limited, energy is provided for babies by adding animal fat and vegetable fat into the milk powder, intake of linoleic acid and other components of the babies is fully considered when the types of the fat are limited, and the linseed oil, sunflower seed oil and other components are added into the milk powder, so that the milk powder is more suitable for the babies. The nutritional requirements of infants are met; in order to further approach breast milk components, the raw milk and the whey powder are subjected to membrane separation process treatment to remove beta-lactoglobulin components in the milk powder, so that the total content of beta-lactoglobulin in the milk powder is reduced, the protein composition and content in the milk powder are effectively optimized, and the formula milk powder is closer to breast milk.

Description

Technical Field

[0001] This invention relates to the field of formula milk powder technology, specifically to an infant formula milk powder with low β-lactoglobulin and its preparation method. Background Technology

[0002] Breast milk is the ideal natural food for infants, providing comprehensive and suitable nutrition for their growth and development. However, due to various realities such as workplace stress and physical conditions faced by modern women, the current breastfeeding rate for infants remains relatively low. Against this backdrop, formula milk powder made primarily from cow's and goat's milk has gradually become a substitute for breast milk to meet the nutritional needs of infants. However, because the lipid composition of cow's and goat's milk differs from that of breast milk, and because cow's and goat's milk contain a large amount of β-lactoglobulin, a secretory protein unique to the mammary epithelial cells of ungulates such as cows, sheep, and horses, whose core function is to serve the offspring of these species, β-lactoglobulin is an "easily absorbed nutrient carrier" for them. But for human infants, it not only has no nutritional value but may also become a "foreign protein," triggering allergies and affecting the infant's gastrointestinal health and even development. Summary of the Invention

[0003] The purpose of this invention is to provide an infant formula milk powder with low β-lactoglobulin and its preparation method, so as to solve the problems mentioned in the background art.

[0004] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a low-β-lactoglobulin infant formula milk powder, having the following technical features: the low-β-lactoglobulin infant formula milk powder is composed of 170-190 parts raw cow's milk, 40-47.5 parts low-β-lactoglobulin demineralized whey powder, 18-21 parts lactose, 5-10 parts animal fat, 25-29 parts mixed vegetable oil, 0.6-1.5 parts DHA, 1.1-2.0 parts ARA, 0.2-0.3 parts calcium powder, 0.2-0.4 parts compound vitamins, and 0.15-0.3 parts compound minerals.

[0005] Furthermore, the calcium powder is calcium carbonate;

[0006] The vegetable oil mixture is obtained by mixing 10-11.5 parts corn oil, 10-10.5 parts low erucic acid rapeseed oil, 1.8-2.5 parts coconut oil, 2-2.5 parts flaxseed oil and 1.2-2 parts sunflower seed oil.

[0007] Furthermore, the animal fat is anhydrous butter.

[0008] Furthermore, each gram of the complex vitamin contains 7500-9500 IU of vitamin A, 2.5-3 mg of vitamin B1, 2-2.5 mg of vitamin B6, and 5-8 μg of vitamin B1.12 350-450mg Vitamin C, 1400-1500U Vitamin D, 50-60mg Vitamin E, 250-300μg Vitamin K1, 450-600μg Folic Acid and 60-80μg Biotin;

[0009] The composite mineral contains 45-55 mg magnesium, 25-30 mg iron, 25-30 mg zinc, 80-110 μg selenium, and 350-450 μg iodine per gram.

[0010] Furthermore, the preparation method of the low-β-lactoglobulin desalted whey powder includes the following steps:

[0011] a. Sterilize the raw milk and centrifuge it for 20-30 minutes to remove the top fat. Collect the lower layer of skimmed milk and concentrate it using an ultrafiltration membrane. After concentration, a milk protein concentrate is obtained.

[0012] b. Mix the milk protein concentrate with 2 to 4 times its volume of deionized water, stir and disperse evenly, add alkali adjuster to adjust the pH value to 11 to 12, stir for 5 to 10 minutes, add acid adjuster again to adjust the pH value to 4.5 to 5.5, continue stirring for 30 to 45 minutes, let stand, and wait for flocculation and precipitation to form before use.

[0013] c. Perform membrane separation on the whey concentrate that produces flocculation and precipitation in step b, and collect the membrane retentate and membrane permeate separately;

[0014] d. After adjusting the pH of the membrane effluent to neutral, stir to reconstitute and obtain a reconstituted solution. After desalting and drying, β-lactoglobulin powder is obtained.

[0015] The permeate was desalted and dried to obtain low-β-lactoglobulin desalted whey powder.

[0016] Furthermore, in step a, during ultrafiltration membrane concentration, the ultrafiltration membrane used has a molecular weight cutoff of 80~120kDa, an inlet pressure of 0.8~1MPa, and a concentration factor of 3~5 times.

[0017] Furthermore, in step b, the alkali regulator is food-grade sodium hydroxide; and the acid regulator is citric acid.

[0018] Furthermore, in step c, the membrane pore size used during membrane separation is 0.2~1.2μm.

[0019] Furthermore, a method for preparing a low-β-lactoglobulin infant formula includes the following steps:

[0020] S1. After pasteurizing raw milk, add low-β-lactoglobulin demineralized whey powder, lactose, animal fat, mixed vegetable fat, DHA, ARA, calcium powder, multivitamins, and multiminerals to it, and then homogenize it under vacuum to obtain a mixed emulsion.

[0021] S2. After vacuum concentrating the mixed emulsion to a dry matter content of 40-55 wt%, spray-dry it and collect the dried product to obtain low β-lactoglobulin infant formula milk powder.

[0022] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0023] In order to make the milk powder closer to the composition of breast milk, the present invention has limited the components and provides energy for infants by adding animal fats and vegetable oils. In addition, when limiting the types of oils, the present invention fully considers the intake of infants' linoleic acid and other components, and adds flaxseed oil, sunflower seed oil and other components to meet the nutritional needs of infants.

[0024] Furthermore, in order to further approximate the composition of breast milk, the present invention also processes the whey powder by performing a membrane separation process to remove the β-lactoglobulin component, thereby reducing the total content of β-lactoglobulin in the milk powder. This effectively reduces the allergic irritation to the infant's intestines caused by foreign proteins such as β-lactoglobulin. This process also optimizes the protein composition and content in the milk powder, making the formula milk powder closer to breast milk. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Example 1. A method for preparing an infant formula milk powder with low β-lactoglobulin content, comprising the following steps:

[0027] S1. By weight, 170 parts of raw milk were pasteurized and then 40 parts of low-β-lactoglobulin demineralized whey powder, 18 parts of lactose, 5 parts of anhydrous butter, 25 parts of mixed vegetable oils, 0.6 parts of DHA powder, 1.1 parts of ARA powder, 0.2 parts of calcium carbonate powder, 0.2 parts of multivitamins, and 0.15 parts of multiminerals were added. After vacuum mixing, the mixture was homogenized to obtain a mixed emulsion.

[0028] The compound vitamin contains, per gram, 7500 IU of vitamin A, 2.5 mg of vitamin B1, 2 mg of vitamin B6, and 5 μg of vitamin B1. 12 400mg Vitamin C, 1500U Vitamin D, 50mg Vitamin E, 250μg Vitamin K1, 600μg Folic Acid and 80μg Biotin;

[0029] The compound mineral contains 45mg magnesium, 30mg iron, 30mg zinc, 110μg selenium, and 450μg iodine per gram.

[0030] The preparation method of the low β-lactoglobulin demineralized whey powder includes the following steps:

[0031] a. Sterilize raw milk and centrifuge at 4500g for 20 minutes to remove the top fat. Collect the lower layer of skimmed milk and concentrate it using an ultrafiltration membrane. The ultrafiltration membrane used has a molecular weight cutoff of 100kDa, an inlet pressure of 0.8MPa, and a concentration factor of 3. After concentration, a milk protein concentrate is obtained.

[0032] b. Mix the milk protein concentrate with three times its volume of deionized water, stir and disperse evenly, add food-grade sodium hydroxide, adjust the pH to 11.5, stir for 5 minutes, add acid adjuster again to adjust the pH to 4.5, continue stirring for 30 minutes, let stand, and wait for flocculation and precipitation to form before use.

[0033] c. Use a filter membrane with a pore size of 0.5 micrometers to perform membrane separation on the whey concentrate that produced flocculation and precipitation in step b, and collect the membrane retentate and membrane permeate separately;

[0034] d. After adjusting the pH of the membrane effluent to neutral, stir to reconstitute and obtain a reconstituted solution. After desalting and drying, β-lactoglobulin powder is obtained.

[0035] The permeate was desalted and dried and concentrated to obtain low-β-lactoglobulin desalted whey powder;

[0036] The vegetable oil blend is made by mixing 10 parts corn oil, 10 parts low erucic acid rapeseed oil, 1.8 parts coconut oil, 2 parts flaxseed oil and 1.2 parts sunflower seed oil;

[0037] S2. After vacuum concentrating the mixed emulsion to a dry matter content of 40 wt%, spray-dry it and collect the dried product to obtain low β-lactoglobulin infant formula milk powder.

[0038] Example 2. A method for preparing an infant formula milk powder with low β-lactoglobulin content, comprising the following steps:

[0039] Compared with Example 1, this example changes the amount of each component added in step S1;

[0040] S1. By weight, 190 parts of raw milk were pasteurized and then 47.5 parts of low β-lactoglobulin demineralized whey powder, 21 parts of lactose, 10 parts of anhydrous butter, 29 parts of mixed vegetable oils, 1.5 parts of DHA powder, 2 parts of ARA powder, 0.3 parts of calcium carbonate powder, 0.4 parts of multivitamins, and 0.3 parts of multiminerals were added. After vacuum mixing, the mixture was homogenized to obtain a mixed emulsion.

[0041] The compound vitamin contains, per gram, 7500 IU of vitamin A, 2.5 mg of vitamin B1, 2 mg of vitamin B6, and 5 μg of vitamin B1. 12 400mg Vitamin C, 1500U Vitamin D, 50mg Vitamin E, 250μg Vitamin K1, 600μg Folic Acid and 80μg Biotin;

[0042] The compound mineral contains 45mg magnesium, 30mg iron, 30mg zinc, 110μg selenium, and 450μg iodine per gram.

[0043] The preparation method of the low β-lactoglobulin demineralized whey powder includes the following steps:

[0044] a. Sterilize raw milk and centrifuge at 4500g for 20 minutes to remove the top fat. Collect the lower layer of skimmed milk and concentrate it using an ultrafiltration membrane. The ultrafiltration membrane used has a molecular weight cutoff of 100kDa, an inlet pressure of 0.8MPa, and a concentration factor of 3. After concentration, a milk protein concentrate is obtained.

[0045] b. Mix the milk protein concentrate with three times its volume of deionized water, stir and disperse evenly, add food-grade sodium hydroxide, adjust the pH to 11.5, stir for 5 minutes, add acid adjuster again to adjust the pH to 4.5, continue stirring for 30 minutes, let stand, and wait for flocculation and precipitation to form before use.

[0046] c. Use a filter membrane with a pore size of 0.5 micrometers to perform membrane separation on the whey concentrate that produced flocculation and precipitation in step b, and collect the membrane retentate and membrane permeate separately;

[0047] d. After adjusting the pH of the membrane effluent to neutral, stir to reconstitute and obtain a reconstituted solution. After desalting and drying, β-lactoglobulin powder is obtained.

[0048] The permeate was desalted and dried and concentrated to obtain low-β-lactoglobulin desalted whey powder;

[0049] The vegetable oil blend is made by mixing 11.5 parts corn oil, 10.5 parts rapeseed oil, 2.5 parts coconut oil, 2.5 parts flaxseed oil and 2 parts sunflower seed oil.

[0050] S2. After vacuum concentrating the mixed emulsion to a dry matter content of 40 wt%, spray-dry it and collect the dried product to obtain low β-lactoglobulin infant formula milk powder.

[0051] Comparative Example 1. A method for preparing an infant formula milk powder with low β-lactoglobulin, comprising the following steps:

[0052] Compared with Example 1, this comparative example only added demineralized whey powder and did not use membrane separation technology to separate β-lactoglobulin;

[0053] S1. By weight, 170 parts of raw milk were pasteurized and then 40 parts of demineralized whey powder, 18 parts of lactose, 5 parts of anhydrous butter, 25 parts of mixed vegetable oil, 0.6 parts of DHA powder, 1.1 parts of ARA powder, 0.2 parts of calcium carbonate powder, 0.2 parts of multivitamins, and 0.15 parts of multiminerals were added. After vacuum mixing, the mixture was homogenized to obtain a mixed emulsion.

[0054] The compound vitamin contains, per gram, 7500 IU of vitamin A, 2.5 mg of vitamin B1, 2 mg of vitamin B6, and 5 μg of vitamin B1. 12 400mg Vitamin C, 1500U Vitamin D, 50mg Vitamin E, 250μg Vitamin K1, 600μg Folic Acid and 80μg Biotin;

[0055] The compound mineral contains 45mg magnesium, 30mg iron, 30mg zinc, 110μg selenium, and 450μg iodine per gram.

[0056] The method for preparing the desalted whey powder includes the following steps:

[0057] a. Sterilize raw milk and centrifuge at 4500g for 20 minutes to remove the top fat. Collect the lower layer of skimmed milk and concentrate it using an ultrafiltration membrane. The ultrafiltration membrane used has a molecular weight cutoff of 100kDa, an inlet pressure of 0.8MPa, and a concentration factor of 3. After concentration, a milk protein concentrate is obtained.

[0058] d. After spray drying the milk protein concentrate, demineralized whey powder is obtained;

[0059] The vegetable oil blend is made by mixing 10 parts corn oil, 10 parts rapeseed oil, 1.8 parts coconut oil, 2 parts flaxseed oil and 1.2 parts sunflower seed oil.

[0060] S2. After vacuum concentrating the mixed emulsion to a dry matter content of 40 wt%, spray-dry it and collect the dried product to obtain low β-lactoglobulin infant formula milk powder.

[0061] Testing: The infant formula milk powders prepared in Examples 1-2 and Comparative Example 1 were tested according to GB 5009.5 for protein content; the β-lactoglobulin content in the infant formula milk powders prepared in Examples 1-2 and Comparative Example 1 was tested according to T / TDSTIA 007-2019, and the test results are shown in the table below.

[0062]

[0063] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A low-β-lactoglobulin infant formula, characterized in that: The low-β-lactoglobulin infant formula milk powder consists of 170-190 parts raw cow's milk, 40-47.5 parts low-β-lactoglobulin demineralized whey powder, 18-21 parts lactose, 5-10 parts animal fat, 25-29 parts mixed vegetable oil, 0.6-1.5 parts DHA, 1.1-2.0 parts ARA, 0.2-0.3 parts calcium powder, 0.2-0.4 parts multivitamins, and 0.15-0.3 parts multiminerals.

2. The infant formula milk powder with low β-lactoglobulin according to claim 1, characterized in that: The calcium powder is calcium carbonate; The vegetable oil mixture is obtained by mixing 10-11.5 parts corn oil, 10-10.5 parts low erucic acid rapeseed oil, 1.8-2.5 parts coconut oil, 2-2.5 parts flaxseed oil and 1.2-2 parts sunflower seed oil.

3. The infant formula milk powder with low β-lactoglobulin according to claim 1, characterized in that: The animal fat is anhydrous butter.

4. The infant formula milk powder with low β-lactoglobulin according to claim 1, characterized in that: The preparation method of the low β-lactoglobulin demineralized whey powder includes the following steps: a. Sterilize the raw milk and centrifuge it for 20-30 minutes to remove the top fat. Collect the lower layer of skimmed milk and concentrate it using an ultrafiltration membrane. After concentration, a milk protein concentrate is obtained. b. Mix the milk protein concentrate with 2 to 4 times its volume of deionized water, stir and disperse evenly, add alkali adjuster to adjust the pH value to 11 to 12, stir for 5 to 10 minutes, add acid adjuster again to adjust the pH value to 4.5 to 5.5, continue stirring for 30 to 45 minutes, let stand, and wait for flocculation and precipitation to form before use. c. Perform membrane separation on the whey concentrate that produces flocculation and precipitation in step b, and collect the membrane retentate and membrane permeate separately; d. After adjusting the pH of the membrane effluent to neutral, stir to reconstitute and obtain a reconstituted solution. After desalting and drying, β-lactoglobulin powder is obtained. The permeate was desalted and dried to obtain low-β-lactoglobulin desalted whey powder.

5. The infant formula milk powder with low β-lactoglobulin according to claim 4, characterized in that: In step a, during ultrafiltration membrane concentration, the ultrafiltration membrane used has a molecular weight cutoff of 80~120kDa, an inlet pressure of 0.8~1MPa, and a concentration factor of 3~5 times.

6. The infant formula milk powder with low β-lactoglobulin according to claim 4, characterized in that: In step b, the alkali regulator is food-grade sodium hydroxide; the acid regulator is citric acid.

7. The infant formula milk powder with low β-lactoglobulin according to claim 4, characterized in that: In step c, the membrane pore size used during membrane separation is 0.2~1.2μm.

8. A method for preparing an infant formula milk powder with low β-lactoglobulin as described in any one of claims 1 to 7, characterized in that, Includes the following steps: S1. After pasteurizing raw milk, add low-β-lactoglobulin demineralized whey powder, lactose, animal fat, mixed vegetable fat, DHA, ARA, calcium powder, multivitamins, and multiminerals to it, and then homogenize it under vacuum to obtain a mixed emulsion. S2. After vacuum concentrating the mixed emulsion to a dry matter content of 40-55 wt%, spray-dry it and collect the dried product to obtain low β-lactoglobulin infant formula milk powder.

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