A composition for improving the penetration effect of the bark extract of *Bretschneidera sinensis*, its preparation method and application

Abstract

This invention provides a composition for improving the penetration effect of *Bretschneidera sinensis* bark extract, its preparation method, and its application, relating to the field of cosmetic technology. The invention provides an anti-aging composition for improving the penetration effect of *Bretschneidera sinensis* bark extract, comprising: recombinant human type III collagen, *Bretschneidera sinensis* bark extract, *Annona squamosa* fruit extract, *Magnolia denudata* flower extract, and a film-forming agent; the film-forming agent is composed of rhamnose and a trimethylpentanediol / adipic acid / glycerol cross-linked polymer. The combination of recombinant human type III collagen and the film-forming agent enhances stratum corneum hydration, optimizes the controlled release efficiency of active ingredients, and broadens the penetration pathway, forming a highly efficient penetration network from the skin surface to the deep layers. The combined use of *Bretschneidera sinensis* bark extract, *Annona squamosa* fruit extract, and *Magnolia denudata* flower extract can construct a multi-dimensional antioxidant network, activate longevity proteins to combat glycation and DNA damage, and comprehensively delay skin aging.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a composition for improving the penetration effect of extracts from the bark of the variegated bellflower tree, its preparation method, and its application. Background Technology

[0002] As research into the biology of skin aging deepens, it has been confirmed that skin aging is a complex process involving both internal mechanisms and the external environment, manifesting as a series of problems such as wrinkle formation, skin laxity, decreased elasticity, impaired barrier function, and reduced radiance. In response to skin aging, the focus of cosmetic research has shifted from traditional surface concealing and basic moisturizing to achieving anti-aging and repair through the addition of highly active ingredients (such as plant extracts, peptides, and vitamins).

[0003] However, the outermost layer of the skin, the stratum corneum, forms a natural physical barrier that protects the body from external stimuli and prevents moisture loss. It also significantly limits the transdermal absorption of active ingredients. Most active substances with a molecular weight greater than 500 Da or those that are hydrophilic struggle to effectively penetrate this "brick wall" structure, resulting in extremely low bioavailability. They cannot reach effective concentrations in the target skin layer (such as the dermis), thus severely restricting the efficacy of the final product.

[0004] Currently, to improve the permeability of active ingredients, chemical penetration enhancers, physical penetration enhancers, and carrier systems are commonly used. Chemical penetration enhancers include azones, alcohols, and fatty acids. While these substances can disrupt the lipid structure of the stratum corneum to increase permeability, they often have potential skin irritation. Long-term use may damage the skin barrier, causing sensitivity, inflammation, and other problems, making it difficult to balance safety and efficacy. Physical penetration enhancers, such as microneedling and ultrasound infusion, are highly effective but complex to operate, expensive, and usually require professional intervention in specialized institutions, making them difficult to apply to everyday home skincare products. Carrier systems, such as liposomes, nanoemulsions, and polymer microparticles, can encapsulate active ingredients, assisting their penetration of the stratum corneum. However, existing carrier systems suffer from complex manufacturing processes or excessively high costs. Furthermore, traditional penetration enhancers often have limited functionality. The market urgently needs a novel solution that not only safely and efficiently penetrates the skin barrier to deliver multiple active ingredients but also ideally possesses certain skincare benefits, thus achieving a perfect fusion of penetration enhancement and efficacy.

[0005] Therefore, developing a novel, multifunctional composition that can improve the transdermal penetration rate of various anti-aging agents without damaging the skin is of great significance for developing next-generation high-performance skincare products. Summary of the Invention

[0006] The purpose of this invention is to provide a composition for improving the penetration effect of the bark extract of the variegated bellflower tree, its preparation method and application. The composition provided by this invention has significant anti-aging effects.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] This invention provides an anti-aging composition for improving the penetration effect of *Bretschneidera sinensis* bark extract, comprising the following raw materials in parts by weight: 0.05-0.2 parts recombinant human type III collagen, 0.05-0.1 parts *Bretschneidera sinensis* bark extract, 0.01-0.03 parts *Annona squamosa* fruit extract, 0.03-0.08 parts *Magnolia denudata* flower extract, and 1.5-2.5 parts film-forming agent; wherein the film-forming agent is composed of rhamnose and a trimethylpentanediol / adipic acid / glycerol crosspolymer.

[0009] Preferably, the weight ratio of rhamnose to trimethylpentanediol / adipic acid / glycerol crosslinking polymer is (0.5-1):(1-1.5).

[0010] Preferably, the extract of *Annona squamosa* fruit is obtained by anaerobic fermentation of *Annona squamosa* fruit using compound microorganisms.

[0011] More preferably, the composite microorganism consists of Lactobacillus plantarum WCFS1 and Bifidobacterium animalis W3.

[0012] Preferably, the Magnolia officinalis flower extract is obtained by extracting Magnolia officinalis flower buds with a eutectic solvent and purifying them with a macroporous adsorption resin.

[0013] More preferably, the eutectic solvent is composed of choline chloride and glycerol.

[0014] The present invention also provides a method for preparing the above-mentioned anti-aging composition, comprising: mixing recombinant human type III collagen, extract of the bark of the variegated bellflower, extract of the flower of the magnolia officinalis, extract of the fruit of the hairy custard apple, rhamnose, trimethylpentanediol / adipic acid / glycerol crosspolymer and water, homogenizing, and obtaining the anti-aging composition.

[0015] Preferably, the homogenization rotation speed is 3000-4000 rpm and the time is 10-15 min.

[0016] The present invention also provides an application of the above composition in the preparation of anti-aging and repair products.

[0017] Preferably, the aging is photoaging and natural aging.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This invention provides an anti-aging composition that enhances the penetration effect of *Bretschneidera sinensis* bark extract, comprising: recombinant human type III collagen, *Bretschneidera sinensis* bark extract, *Annona squamosa* fruit extract, *Magnolia denudata* flower extract, and a film-forming agent; the film-forming agent is composed of rhamnose and a trimethylpentanediol / adipic acid / glycerol cross-linked polymer. The combined use of recombinant human type III collagen and the film-forming agent strengthens stratum corneum hydration, optimizes the controlled release efficiency of active ingredients, and broadens the penetration pathway, forming a highly efficient penetration network from the skin surface to the deep layers. The combined use of *Bretschneidera sinensis* bark extract, *Annona squamosa* fruit extract, and *Magnolia denudata* flower extract constructs a multi-dimensional antioxidant network, promoting collagen synthesis and inhibiting degradation to protect the supporting structure, activating longevity proteins to combat glycation and DNA damage, and comprehensively delaying skin aging (wrinkles, sagging, dullness); through multi-target anti-inflammatory and soothing effects, it rebuilds the entire cellular barrier from the stratum corneum to the deep cellular layers, supporting wound healing and improving tolerance, making it suitable for sensitive skin, damaged skin, and post-medical aesthetic care. The composition of this invention enhances the transdermal efficiency and anti-aging efficacy of active ingredients through multi-component compounding, and can be widely used in the fields of skin care products or anti-aging drugs to achieve better skin repair and rejuvenation effects. Detailed Implementation

[0020] This invention provides an anti-aging composition for improving the penetration effect of *Bretschneidera sinensis* bark extract, comprising the following raw materials in parts by weight: 0.05-0.2 parts recombinant human type III collagen, 0.05-0.1 parts *Bretschneidera sinensis* bark extract, 0.01-0.03 parts *Annona squamosa* fruit extract, 0.03-0.08 parts *Magnolia denudata* flower extract, and 1.5-2.5 parts film-forming agent; wherein the film-forming agent is composed of rhamnose and a trimethylpentanediol / adipic acid / glycerol crosspolymer.

[0021] Compared to traditional chemical penetration enhancers (such as alcohol and propylene glycol), the recombinant human type III collagen of this invention is gentler in promoting penetration. It binds to keratinocytes in the stratum corneum through hydrogen bonds, adsorbs water molecules to form a hydrating microenvironment, and widens the penetration channels. It can also encapsulate the bark extract of *Bretschneidera sinensis* and deliver it to the dermis without damaging the lipid structure of the stratum corneum. In addition to its penetration-enhancing effect, it has high homology with human collagen, is non-allergenic, promotes keratinocyte proliferation and differentiation, and synergistically repairs the skin barrier with extracts of *Annona squamosa* fruit and *Magnolia denudata* flower. It also replenishes type III collagen in the dermis and synergizes with the "inhibition of collagen degradation" effect of *Bretschneidera sinensis* bark extract, strengthening the anti-aging effect. It is suitable for sensitive skin and post-medical aesthetic care, avoiding the dryness and irritation problems of traditional chemical penetration enhancers.

[0022] The weight ratio of rhamnose and trimethylpentanediol / adipic acid / glycerol crosslinked polymer described in this invention is preferably (0.5-1):(1-1.5), more preferably 0.8:1.2.

[0023] The rhamnose of this invention has film-forming and moisturizing effects, enhancing the skin barrier's moisturizing ability. It forms a breathable film on the skin surface, which not only reduces water evaporation but also increases the residence time of other active ingredients (such as *Bretschneidera sinensis* bark extract) on the skin surface, thereby promoting their gradual penetration. Rhamnose also has soothing properties, reducing the impact of external stimuli on the skin and creating a more stable environment for the penetration of active ingredients. The trimethylpentanediol / adipic acid / glycerol crosspolymer has excellent film-forming properties and stability. The film it forms has a balance of breathability and flexibility, can encapsulate lipid-soluble or water-soluble active ingredients, has a controlled-release effect, and enhances the adhesion of active ingredients to the skin surface, reducing the loss of active ingredients due to evaporation or rinsing, indirectly improving penetration efficiency. The film-forming agent of this invention can form a breathable film on the skin surface, maintaining stratum corneum hydration by locking in water, softening the skin structure to promote the penetration of active ingredients. At the same time, its adhesiveness can prolong the residence time of active substances, achieving continuous release and absorption, thereby synergistically enhancing the penetration effect of ingredients such as *Bretschneidera sinensis* bark extract.

[0024] Recombinant human type III collagen and film-forming agents exhibit a synergistic effect in enhancing skin penetration: Recombinant human type III collagen itself possesses excellent skin affinity and moisturizing properties, softening the stratum corneum and creating favorable conditions for the penetration of other active ingredients. When the film-forming agent (a cross-linked polymer of rhamnose and trimethylpentanediol / adipic acid / glycerol) forms a film on the skin surface, it not only locks in moisture and maintains a high hydration state but also prolongs the contact time between the active ingredients and the skin through a sustained-release effect. The combined action of collagen and the film-forming agent further enhances the permeability of the stratum corneum, thereby significantly improving the penetration efficiency and bioavailability of active ingredients such as the bark extract of *Bretschneidera sinensis*.

[0025] The preferred extract of *Bretschneidera sinensis* bark in this invention is *Bretschneidera sinensis* bark extract 1 as described in patent CN119837808B. The preparation method of *Bretschneidera sinensis* bark extract 1 is as follows: S1. Air-dry *Bretschneidera sinensis* bark until the moisture content is ≤5%, and grind it into coarse powder; S2. Mix the coarse powder of *Bretschneidera sinensis* bark with rapeseed oil and polyglycerol-3 diisostearate, heat to 55℃, and stir at 300 rpm for 20 min to obtain a mixture; the mass ratio of *Bretschneidera sinensis* bark powder, rapeseed oil, and polyglycerol-3 diisostearate is 1:3:0.2; S3. Under N2 atmosphere, perform ultrasonic-assisted extraction on the mixture in S2 to obtain the extract; extraction temperature: 55℃, ultrasonic frequency: 30 kHz, extraction time: 45 min; S4. Filter the extract to remove insoluble matter, centrifuge, and collect the supernatant to obtain the *Bretschneidera sinensis* bark extract.

[0026] The bark extract of *Bretschneidera sinensis* of this invention contains polyphenols (such as flavonoids and tannins), polyols, and terpenoids. These compounds can directly scavenge free radicals (ROS, hydroxyl radicals), inhibit lipid peroxidation, provide electrons to neutralize oxidatively active molecules, and inhibit collagen degradation. Furthermore, they can activate Sirtuins family proteins, achieving DNA repair, anti-glycation, telomere maintenance, and inflammation regulation, thus achieving anti-aging effects. They also have repairing effects on deep cells, strengthening the skin barrier, and soothing and anti-inflammatory properties. Applying the *Bretschneidera sinensis* bark extract of this invention to skincare products can achieve comprehensive effects such as reducing wrinkles, firming the skin, improving dullness, and enhancing skin resilience and radiance.

[0027] The extract of *Annona squamosa* fruit described in this invention is preferably obtained from *Annona squamosa* fruit through anaerobic fermentation using a compound microorganism. The compound microorganism is preferably composed of *Lactobacillus plantarum* WCFS1 and *Bifidobacterium animalis* W3. *Lactobacillus plantarum* WCFS1 was purchased from the China Center for Type Culture Collection (CCTCC NO: M2021228) and is disclosed in patent CN115305217A. The preferred bacterial count in the compound microorganism is (6-10) × 10⁶. 8 8 × 10⁶ cells / g, more preferably 8 × 10⁶ cells / g. 8 / g; Bifidobacterium animalis W3 was purchased from the China General Microbiological Culture Collection Center, accession number CGMCC No. 25262, disclosed in patent CN117224462A, and the preferred bacterial count in the composite microorganism is (3-7)×10⁻⁶. 8 5 × 10⁻⁶ cells / g, more preferably 5 × 10⁻⁶ cells 8 The inoculation amount of the compound microorganisms is preferably 3%-5% of the weight of the slurry, more preferably 4%. The preferred preparation method of the hairy custard apple fruit extract includes: using a high-speed blender to intermittently crush fresh hairy custard apple whole fruit at 20000-30000 rpm for 30 seconds, pausing for 30 seconds, repeating 3-4 times to obtain a slurry, adding 12-17 times the weight of the slurry of water, sterilizing, inoculating with compound microorganisms, anaerobic fermenting at 35-40℃ and pH 6-7 for 56-72 hours, sterilizing by ultraviolet light irradiation, filtering with a 0.22μm membrane, and vacuum freeze-drying the filtrate to obtain the hairy custard apple fruit extract.

[0028] This invention involves fermenting *Lactobacillus plantarum* WCFS1 and *Bifidobacterium animalis* W3 together with *Annona squamosa* fruit. This process decomposes and transforms the components of the fruit, enriching the variety of active ingredients in the extract and increasing the content of active ingredients (such as fermented polyphenols, short-chain fatty acids, and active peptides). The preparation of this *Annona squamosa* fruit extract achieves efficient retention of active ingredients and enhanced functional efficacy. In anti-aging, it delays aging through anti-oxidation, collagen synthesis promotion, and moisturizing and wrinkle reduction. In repair, it supports skin recovery through anti-inflammatory soothing, barrier reconstruction, and wound healing. It possesses dual anti-aging and repairing value, making it suitable for skincare products, medical dressings, and other fields, especially for early signs of aging, sensitive skin, and post-cosmetic surgery care.

[0029] The preferred method for preparing the Magnolia officinalis flower extract of this invention includes: pulverizing dried Magnolia officinalis flower buds through a 50-100 mesh sieve to obtain Magnolia officinalis pollen; adding a eutectic solvent aqueous solution at a ratio of 1:15-20 g / mL; extracting at 60-70℃ and 200-400 rpm for 60-90 min; cooling to 25-30℃; and centrifuging at 8000-10000 rpm for 10-15 min to obtain the supernatant; and further processing the supernatant according to a 1.5- After adsorption at a flow rate of 2.5 BV / h using an AB-8 macroporous adsorption resin column, the extract is eluted with 3-5 BV of water (flow rate 2 BV / h) to remove eutectic solvents, sugars, proteins, and other highly polar impurities. Then, it is eluted with 4-6 BV of a 68%-72% ethanol solution (flow rate 1-2 BV / h). The eluent containing the 68%-72% ethanol solution is collected and freeze-dried under vacuum to a water content of 2wt%-5wt% to obtain the Magnolia officinalis flower extract. The preferred method for preparing the eutectic solvent aqueous solution includes: mixing choline chloride and glycerol at a molar ratio of 1:1.5-2.5 and stirring at 200-400 rpm and 70-80°C for 50-60 min to obtain the eutectic solvent; mixing the eutectic solvent and water at a weight ratio of (7-8):(2-3) and stirring at 200-400 rpm for 5-10 min to obtain the eutectic solvent aqueous solution.

[0030] Compared to traditional methods, this invention uses a eutectic solvent to prepare Magnolia officinalis flower extract, achieving higher purity and activity in a more environmentally friendly, efficient, and gentle manner. The Magnolia officinalis flower extract of this invention is rich in lignans (such as magnolin and magnolol), volatile oils, and flavonoids, exhibiting free radical scavenging, antioxidant, matrix metalloproteinase inhibitory, collagen-protecting, and cell-aging-delaying effects. It also possesses anti-inflammatory, soothing, barrier-repairing, and elastase-inhibiting repairing properties. Therefore, the Magnolia officinalis flower extract of this invention can effectively prevent and reduce wrinkles, improve firmness, improve rough and dull skin, and enhance skin tolerance, achieving highly effective anti-aging and repair.

[0031] This invention utilizes extracts from the bark of *Bretschneidera sinensis*, the fruit of *Annona squamosa*, and the flower of *Magnolia denudata* as active ingredients in an anti-aging composition. The combination of these three ingredients comprehensively scavenges free radicals, reduces oxidative damage, and enhances antioxidant effects. *Bretschneidera sinensis* bark extract reduces collagen degradation by inhibiting collagenase activity; *Magnolia denudata* flower extract specifically inhibits matrix metalloproteinases and elastases, preventing abnormal degradation of collagen and elastin; and *Annona squamosa* fruit extract promotes collagen and elastin synthesis. *Bretschneidera sinensis* bark extract can reduce skin yellowing and stiffness caused by the accumulation of advanced glycation end products (AGEs); *Magnolia denudata* flower extract can prolong the lifespan of epidermal cells and dermal fibroblasts; and *Annona squamosa* fruit extract can improve cellular energy metabolism, thereby… Delaying skin aging; the bark extract of *Bonbour glauca* can soothe nerve endings and reduce sensitivity symptoms such as redness and burning; the fruit extract of *Annona squamosa* can regulate the skin flora balance, reduce inflammation, and relieve itching and erythema; the flower extract of *Magnolia denudata* can quickly soothe acute inflammatory reactions and enhance skin immune tolerance. The three exert anti-inflammatory effects through multiple mechanisms such as inhibiting inflammatory factors, regulating flora, and enhancing tolerance; the fruit extract of *Annona squamosa* promotes the proliferation and differentiation of keratinocytes and accelerates the reconstruction of the stratum corneum; the flower extract of *Magnolia denudata* can enhance the barrier's water-locking ability; the bark extract of *Bonbour glauca* can reduce transdermal water loss, improve the dermal-epidermal junction structure, and enhance skin resilience. The combination of the three can achieve full structural reconstruction from the stratum corneum to the deep cells. As can be seen from the above, the combination of these three ingredients can construct a multi-dimensional antioxidant network, promoting collagen synthesis and inhibiting degradation to protect the supporting structure, activating longevity proteins to combat glycation and DNA damage, and comprehensively delaying skin aging (wrinkles, sagging, dullness); through multi-target anti-inflammatory and soothing effects, it can rebuild the barrier from the stratum corneum to the deep cells, supporting wound healing and improving tolerance, and is suitable for sensitive skin, damaged skin, and post-medical aesthetic care.

[0032] The anti-aging composition of the present invention preferably includes fibroin and mannan.

[0033] The anti-aging composition of the present invention preferably includes a moisturizer, an emulsifier, and a preservative, all of which are selected from raw materials known in the cosmetics field.

[0034] This invention also provides a method for preparing the anti-aging composition described above, comprising: mixing recombinant human type III collagen, *Bretschneidera sinensis* bark extract, *Magnolia denudata* flower extract, *Annona squamosa* fruit extract, rhamnose, trimethylpentanediol / adipic acid / glycerol crosspolymer, and water, and homogenizing to obtain the anti-aging composition. The homogenization speed is preferably 3000-4000 rpm, more preferably 3500 rpm, and the homogenization time is preferably 10-15 min, more preferably 12 min.

[0035] The present invention also provides an application of the above composition in the preparation of anti-aging and repair products, wherein the aging is photoaging and natural aging.

[0036] The technical solutions of this invention will be clearly and completely described below with reference to the embodiments thereof. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0037] Unless otherwise specified, the following embodiments are all conventional methods.

[0038] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0039] Some of the raw materials and their sources are as follows:

[0040] The bark extract of the variegated bellflower tree is the variegated bellflower tree bark extract 1 in patent CN119837808B;

[0041] Lactobacillus plantarum WCFS1: purchased from China Center for Type Culture Collection, accession number CCTCC NO:M2021228, and published in patent CN115305217A;

[0042] Bifidobacterium animalis W3: purchased from China General Microbiological Culture Collection Center, accession number CGMCC No. 25262, and disclosed in patent CN117224462A;

[0043] Recombinant human type III collagen: purchased from Xi'an Baiwangda Pharmaceutical Technology Co., Ltd.;

[0044] Rhamnose: L-rhamnose, purchased from Xi'an Nanpu Biotechnology Co., Ltd.;

[0045] Trimethylpentanediol / adipic acid / glycerol crosspolymer: purchased from Shanghai Zibang Biopharmaceutical Co., Ltd.;

[0046] Lactobacillus delbrueckii WHH3887: purchased from China Culture Collection Center for Microbial Cultures, accession number CGMCCNo.20090, and published in patent CN112625971A;

[0047] Bifidobacterium adolescentis ZJ2: purchased from China Culture Collection Center for Microbial Cultures, accession number CGMCC NO.18901, published in patent CN112725219A;

[0048] The hydroxyl radical scavenging ability test kit, the ABTS radical scavenging ability test kit, and the superoxide anion scavenging ability test kit were all purchased from Beijing Solarbio Science & Technology Co., Ltd.

[0049] The mouse superoxide dismutase (SOD) ELISA kit, mouse glutathione peroxidase (GSH-Px) ELISA kit, mouse catalase (CAT) ELISA kit, and mouse malondialdehyde (MDA) ELISA kit were all purchased from Shanghai Keaibo Biotechnology Co., Ltd.

[0050] Example 1

[0051] Preparation of anti-aging compositions

[0052] (1) Preparation of extract from hairy soursop fruit

[0053] Fresh whole fruits of *Annona squamosa* were intermittently crushed at 25,000 rpm for 30 seconds using a high-speed blender, then paused for 30 seconds. This process was repeated four times to obtain a slurry. Water with a weight of 15 times the slurry was added, and the mixture was sterilized. Then, a compound microbial culture was inoculated, and the mixture was anaerobic fermented at 37°C and pH 6.5 for 64 hours. The mixture was then sterilized by UV irradiation, filtered through a 0.22 μm membrane, and the filtrate was freeze-dried under vacuum to a water content of 3 wt% to obtain *Annona squamosa* fruit extract.

[0054] The compound microorganisms consist of *Lactobacillus plantarum* WCFS1 and *Bifidobacterium animalis* W3, with the *Lactobacillus plantarum* WCFS1 having a bacterial count of 8 × 10⁻⁶. 8 The number of Bifidobacterium animalis W3 cells / g was 5 × 10⁻⁶. 8 The inoculum size is 4% of the slurry weight, with each microorganism inoculated at a rate of 1 / g.

[0055] (2) Preparation of Magnolia officinalis flower extract

[0056] The dried Magnolia officinalis flower buds were pulverized and passed through an 80-mesh sieve to obtain Magnolia officinalis pollen. A eutectic solvent aqueous solution was added at a ratio of 1:18 g / mL, and the mixture was extracted at 65℃ and 300 rpm for 75 min. After cooling to 28℃, the mixture was centrifuged at 9000 rpm for 12 min to obtain the supernatant. The supernatant was then adsorbed onto an AB-8 macroporous adsorption resin column at a flow rate of 2 BV / h, eluted with 4 BV of water (flow rate 2 BV / h), and then eluted with 5 BV of 70% ethanol solution (flow rate 1.5 BV / h). The eluent containing the 70% ethanol solution was collected and freeze-dried under vacuum to a water content of 3 wt% to obtain the Magnolia officinalis flower extract.

[0057] The preparation of the eutectic solvent aqueous solution is as follows: choline chloride and glycerol are mixed at a molar ratio of 1:2 and stirred at 300 rpm and 75°C for 55 min to obtain the eutectic solvent. The eutectic solvent is then mixed with water at a weight ratio of 7.5:2.5 and stirred at 300 rpm for 8 min to obtain the eutectic solvent aqueous solution.

[0058] (3) Preparation of anti-aging composition

[0059] Accurately weigh the following components per 100 parts by weight: 0.15 parts recombinant human type III collagen, 0.08 parts *Cephalotaxus fortunei* bark extract, 0.02 parts *Annona squamosa* fruit extract, 0.05 parts *Magnolia denudata* flower extract, 0.8 parts rhamnose, 1.2 parts trimethylpentanediol / adipic acid / glycerol crosspolymer, 4 parts glycerol, 8 parts squalane / caprylic / capric triglyceride, 2 parts stearyl alcohol polyether-21, 0.8 parts stearyl alcohol polyether-2, 0.5 parts phenoxyethanol, 0.25 parts ethylhexylglycerin, and the balance deionized water;

[0060] Glycerin, rhamnose, trimethylpentanediol / adipic acid / glycerin crosspolymer, and deionized water were stirred at 72°C and 250 rpm for 18 min to obtain an aqueous phase. Squalane / caprylic / capric triglyceride, stearyl alcohol polyether-21, and stearyl alcohol polyether-2 were dissolved at 78°C for 8 min to obtain an oil phase. The oil phase and aqueous phase were mixed and homogenized at 3500 rpm for 12 min to obtain a primary emulsion. After cooling to 40°C, extracts of *Bretschneidera sinensis* bark, *Annona squamosa* fruit, and *Magnolia denudata* flower were added, along with recombinant human type III collagen, phenoxyethanol, and ethylhexylglycerin. The pH was adjusted to 6.2 to obtain the anti-aging composition.

[0061] Example 2

[0062] Preparation of anti-aging compositions

[0063] (1) Preparation of extract from hairy soursop fruit

[0064] Fresh whole fruit of *Annona squamosa* was processed in an intermittent blender at 20,000 rpm for 30 seconds, paused for 30 seconds, and repeated 4 times to obtain a slurry. Water with 12 times the weight of the slurry was added, and the mixture was sterilized. Then, it was inoculated with a compound microorganism and anaerobic fermented at 35°C and pH 6 for 72 hours. The mixture was then sterilized by UV irradiation, filtered through a 0.22 μm membrane, and the filtrate was freeze-dried under vacuum to a water content of 2 wt% to obtain *Annona squamosa* fruit extract.

[0065] The compound microorganisms consist of *Lactobacillus plantarum* WCFS1 and *Bifidobacterium animalis* W3, with the *Lactobacillus plantarum* WCFS1 having a bacterial count of 6 × 10⁻⁶. 8 The number of Bifidobacterium animalis W3 cells / g is 3 × 10⁻⁶. 8The inoculum size is 5% of the slurry weight, and the inoculum size of the compound microorganisms is 5%.

[0066] (2) Preparation of Magnolia officinalis flower extract

[0067] The dried Magnolia officinalis flower buds were pulverized and passed through a 50-mesh sieve to obtain Magnolia officinalis pollen. A eutectic solvent aqueous solution was added at a ratio of 1:15 g / mL, and the mixture was extracted at 60℃ and 200 rpm for 90 min. After cooling to 25℃, the mixture was centrifuged at 8000 rpm for 15 min to obtain the supernatant. The supernatant was then adsorbed onto an AB-8 macroporous adsorption resin column at a flow rate of 1.5 BV / h, eluted with 3 BV of water (flow rate 2 BV / h), and then eluted with 4 BV of 68% ethanol solution (flow rate 1 BV / h). The eluent of the 68% ethanol solution was collected and freeze-dried under vacuum to a water content of 2 wt% to obtain the Magnolia officinalis flower extract.

[0068] The preparation of the eutectic solvent aqueous solution is as follows: choline chloride and glycerol are mixed at a molar ratio of 1:1.5 and stirred at 200 rpm and 70°C for 60 min to obtain the eutectic solvent. The eutectic solvent is then mixed with water at a weight ratio of 7:3 and stirred at 200 rpm for 10 min to obtain the eutectic solvent aqueous solution.

[0069] (3) Preparation of anti-aging composition

[0070] Accurately weigh the following components per 100 parts by weight: 0.05 parts recombinant human type III collagen, 0.05 parts *Cephalotaxus fortunei* bark extract, 0.03 parts *Annona squamosa* fruit extract, 0.03 parts *Magnolia denudata* flower extract, 1 part rhamnose, 1.5 parts trimethylpentanediol / adipic acid / glycerol crosspolymer, 1.5 parts panthenol, 5 parts squalane, 2 parts PEG-100 stearate, 0.8 parts benzyl alcohol, and the balance being deionized water;

[0071] Panthenol, rhamnose, trimethylpentanediol / adipic acid / glycerol crosspolymer, and deionized water were stirred at 70°C and 200 rpm for 20 min to obtain an aqueous phase. Squalane and PEG-100 stearate were dissolved at 75°C for 10 min to obtain an oil phase. The oil phase and aqueous phase were mixed and homogenized at 3000 rpm for 15 min to obtain a primary emulsion. After cooling to 38°C, extracts of *Bretschneidera sinensis* bark, *Annona squamosa* fruit, and *Magnolia denudata* flower, recombinant human type III collagen, and benzyl alcohol were added, and the pH was adjusted to 6 to obtain an anti-aging composition.

[0072] Example 3

[0073] Preparation of anti-aging compositions

[0074] (1) Preparation of extract from hairy soursop fruit

[0075] Fresh whole fruit of *Annona squamosa* was processed in an intermittent blender at 30,000 rpm for 30 seconds, paused for 30 seconds, and repeated three times to obtain a slurry. 17 times the weight of water was added to the slurry, and after sterilization, it was inoculated with compound microorganisms and anaerobic fermented at 40℃ and pH 7 for 56 hours. After sterilization by UV irradiation, the filtrate was filtered through a 0.22μm membrane and then freeze-dried under vacuum to a water content of 4wt% to obtain *Annona squamosa* fruit extract.

[0076] The compound microorganism consists of *Lactobacillus plantarum* WCFS1 and *Bifidobacterium animalis* W3, with the *Lactobacillus plantarum* WCFS1 having a bacterial count of 1 × 10⁻⁶. 9 The number of Bifidobacterium animalis W3 cells / g was 7 × 10⁻⁶. 8 The inoculum size is 3% of the slurry weight, with each microorganism inoculated at a rate of 1 / g.

[0077] (2) Preparation of Magnolia officinalis flower extract

[0078] The dried Magnolia officinalis flower buds were crushed and passed through a 100-mesh sieve to obtain Magnolia officinalis pollen. A eutectic solvent aqueous solution was added at a ratio of 1:20 g / mL, and the mixture was extracted at 70℃ and 400 rpm for 60 min. After cooling to 30℃, the mixture was centrifuged at 10000 rpm for 10 min to obtain the supernatant. The supernatant was then adsorbed onto an AB-8 macroporous adsorption resin column at a flow rate of 2.5 BV / h, eluted with 5 BV of water (flow rate 2 BV / h), and then eluted with 6 BV of 72% ethanol solution (flow rate 2 BV / h). The eluent containing the 72% ethanol solution was collected and freeze-dried under vacuum to a water content of 3 wt% to obtain the Magnolia officinalis flower extract.

[0079] The preparation of the eutectic solvent aqueous solution is as follows: choline chloride and glycerol are mixed at a molar ratio of 1:2.5 and stirred at 400 rpm and 80°C for 50 min to obtain the eutectic solvent. The eutectic solvent is then mixed with water at a weight ratio of 8:2 and stirred at 400 rpm for 5 min to obtain the eutectic solvent aqueous solution.

[0080] (3) Preparation of anti-aging composition

[0081] Accurately weigh the following components per 100 parts by weight: 0.18 parts recombinant human type III collagen, 0.1 parts *Cephalotaxus fortunei* bark extract, 0.01 parts *Annona squamosa* fruit extract, 0.08 parts *Magnolia denudata* flower extract, 0.5 parts rhamnose, 1 part trimethylpentanediol / adipic acid / glycerin crosspolymer, 3 parts glycerin, 2 parts petrolatum, 1.5 parts lanolin, 4 parts caprylic / capric triglyceride, 1.2 parts cetearyl alcohol polyether-20, 1 part cetearyl alcohol, 0.5 parts phenoxyethanol, 0.3 parts ethylhexylglycerin, and the balance deionized water;

[0082] Glycerin, rhamnose, trimethylpentanediol / adipic acid / glycerin crosspolymer, and deionized water were stirred at 75°C and 300 rpm for 15 min to obtain an aqueous phase. Petrolatum, lanolin, caprylic / capric triglyceride, cetearyl alcohol polyether-20, and cetearyl alcohol were dissolved at 78°C for 8 min to obtain an oil phase. The oil phase was mixed with the aqueous phase and homogenized at 4000 rpm for 10 min to obtain a primary emulsion. After cooling to 40°C, extracts of *Bretschneidera sinensis* bark, *Annona squamosa* fruit, and *Magnolia denudata* flower were added, along with recombinant human type III collagen, phenoxyethanol, and ethylhexylglycerin. The pH was adjusted to 6.5 to obtain the anti-aging composition.

[0083] Comparative Example 1

[0084] Unlike Example 1, recombinant human type III collagen was omitted, and the weight of rhamnose was adjusted to 0.86 parts, while the weight of trimethylpentanediol / adipic acid / glycerol crosspolymer was adjusted to 1.29 parts.

[0085] Comparative Example 2

[0086] Unlike Example 1, the rhamnose and trimethylpentanediol / adipic acid / glycerol crosspolymer were omitted, and the weight of recombinant human type III collagen was adjusted to 2.15 parts.

[0087] Comparative Example 3

[0088] Unlike Example 1, recombinant human type III collagen was replaced with laurocapram.

[0089] Comparative Example 4

[0090] Unlike Example 1, in step (1), the composite microorganism consists of Lactobacillus delbrueckii WHH3887 and Bifidobacterium adolescentis ZJ2, and the Lactobacillus delbrueckii WHH3887 count in the composite microorganism is 8 × 10⁻⁶. 8 The number of Bifidobacterium adolescentis ZJ2 cells / g is 5 × 10⁻⁶. 8 The number of microorganisms per gram was 4% of the slurry weight, with other conditions remaining unchanged.

[0091] Comparative Example 5

[0092] Unlike Example 1, in step (2), the eutectic solvent aqueous solution is replaced with a 70% volume fraction ethanol solution.

[0093] Experimental Example 1

[0094] In vitro antioxidant assay

[0095] The anti-aging compositions of Examples 1-3 and Comparative Examples 1-5 were mixed with deionized water at a volume ratio of 1:1 to obtain the test samples.

[0096] The hydroxyl radical scavenging rate and ABTS of the test samples in Examples 1-3 and Comparative Examples 1-5 were determined respectively. + Free radical scavenging rate and superoxide free radical scavenging rate.

[0097] Hydroxyl radical scavenging rate and ABTS were detected by spectrophotometry according to the kit instructions. + Free radical scavenging rate and superoxide free radical scavenging rate.

[0098] Hydroxyl radical scavenging rate and ABTS of the anti-aging compositions tested in Examples 1-3 and Comparative Examples 1-5 + The results of free radical scavenging rate and superoxide free radical scavenging rate are shown in Table 1.

[0099] Table 1. Antioxidant capacity of each anti-aging composition

[0100] Grouping Hydroxyl radical scavenging rate (%) ABTS+ free radical scavenging rate (%) Superoxide radical scavenging rate (%) Example 1 92.52 94.27 88.33 Example 2 90.17 92.53 86.71 Example 3 91.31 93.16 87.58 Comparative Example 1 69.80 71.51 67.90 Comparative Example 2 82.65 86.82 81.86 Comparative Example 3 75.98 77.68 73.54 Comparative Example 4 80.24 82.35 78.12 Comparative Example 5 83.56 85.14 81.45

[0101] Table 1 shows that Examples 1-3 exhibited the strongest antioxidant capacity. A comparison between Example 1 and Comparative Examples 1-3 indicates that discarding or replacing recombinant human type III collagen reduced its antioxidant performance, suggesting that recombinant human type III collagen possesses certain antioxidant properties. A comparison between Example 1 and Comparative Examples 4-5 shows that specific complex microorganisms influence the activity of the fermentation extract; low-eutectic solvent extraction is more efficient and better enhances the antioxidant properties of the extract.

[0102] Experimental Example 2

[0103] Animal experiments on natural aging

[0104] SPF-grade 8-week-old Kunming mice weighing 18-22g were selected. After 1 week of acclimatization feeding, the fur on the back of the neck of the mice was shaved off, covering an area of ​​about 2cm × 2cm. 72 hours after shaving, the mice were divided into 11 groups of 10 mice each, with half males and half females. There were no significant differences in weight, health status, etc. among the groups.

[0105] The grouping is as follows:

[0106] Blank control group: The neck and back were injected subcutaneously with physiological saline at a dose of 200 mg / kg every day, and 0.1 mL of distilled water was applied to the shaved area every day.

[0107] Model group: D-galactose was injected into the back of the neck at a dose of 200 mg / kg daily, and 0.1 mL of distilled water was applied to the shaved area daily.

[0108] Positive control group: D-galactose was injected into the back of the neck daily at a dose of 200 mg / kg, and 0.1 mL of 5% vitamin E cream was applied to the shaved area daily.

[0109] Examples 1-3: D-galactose was injected into the back of the neck at a dose of 200 mg / kg daily, and 0.1 mL of the corresponding anti-aging composition was applied to the shaved area daily.

[0110] Comparative groups 1-5: D-galactose was injected into the back of the neck daily at a dose of 200 mg / kg, and 0.1 mL of the corresponding anti-aging composition was applied to the shaved area daily.

[0111] The injection and application were repeated for 8 weeks. After the last application, the patient was kept on a fasting diet but allowed free access to water for 12 hours. Blood was collected from the eyeballs and placed in centrifuge tubes. After standing, the blood was centrifuged (3000 rpm, 10 min) to separate the serum for the determination of malondialdehyde (MDA).

[0112] The mice were then euthanized, and the skin tissue from the shaved area was quickly cut off, rinsed with pre-cooled physiological saline to remove bloodstains and adhering substances, and blotted dry with filter paper. 0.1 g of skin tissue was mixed with 0.9 mL of 4℃ PBS solution (pH 7.4), homogenized at 3000 rpm for 15 s under ice bath conditions, and repeated 4 times to obtain a skin tissue homogenate.

[0113] The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in skin tissue homogenate were detected according to the kit instructions, and the content of malondialdehyde (MDA) in serum was also detected.

[0114] The results of SOD activity, GSH-Px activity, CAT activity in skin tissue and MDA content in serum are shown in Table 2.

[0115] Table 2. SOD activity, GSH-Px activity, CAT activity, and MDA content in each group of mice.

[0116] Group SOD (U / mL) GSH-Px (U / mL) CAT (U / mL) MDA (nmol / mL) Blank control group 98.27 903.23 11.32 5.37 Model group 55.18 437.81 5.02 13.80 Positive control group 90.61 836.12 10.20 5.66 Example 1 Group 93.45 842.67 10.57 5.55 Example 2 group 92.80 840.51 10.39 5.79 Example 3 Group 91.07 838.05 10.28 5.71 Comparative Example 1 72.24 694.98 7.36 9.24 Comparative Example 2 74.36 705.70 7.53 9.03 Comparative Example 3 77.92 721.34 7.95 8.89 Comparative Example 4 83.59 757.06 8.41 8.48 Comparative Example 5 86.73 769.49 8.74 7.91

[0117] Table 2 shows that, compared with the blank control group, the activity of SOD, GSH-Px, and CAT in the skin tissue of the model group was significantly reduced, indicating that the mouse natural aging model was successfully constructed. Examples 1-3 were close to or better than the positive control group, demonstrating that the composition of this invention has good anti-aging ability. A comparison of Example 1 and Comparative Examples 1-2 shows that recombinant human type III collagen and the film-forming agent have a synergistic effect; the combination of the two promotes the penetration of the active ingredients while controlling the release efficiency, allowing the active ingredients to penetrate the skin efficiently. A comparison of Example 1 and Comparative Example 3 shows that a suitable penetration enhancer can improve the anti-aging effect of the body. A comparison of Example 1 and Comparative Examples 4-5 shows that the extraction of raw materials using specific composite microorganisms and solvents can affect the anti-aging effect.

[0118] Experimental Example 3

[0119] Photoaging animal experiments

[0120] SPF-grade 8-week-old Kunming mice weighing 18-22g were selected. After 1 week of acclimatization feeding, the fur on the back of the neck of the mice was shaved off, covering an area of ​​about 2cm × 2cm. 72 hours after shaving, the mice were divided into 11 groups of 10 mice each, with half males and half females. There were no significant differences in weight, health status, etc. among the groups.

[0121] The grouping is as follows:

[0122] Blank control group: 0.1 mL of distilled water was applied to the shaved area 1 hour after each day's ultraviolet irradiation;

[0123] Model group: Apply 0.1 mL of distilled water to the shaved area 1 hour after daily UV exposure;

[0124] Positive control group: 0.1 mL of 5% vitamin E cream was applied to the shaved area 1 hour after daily ultraviolet irradiation;

[0125] Examples 1-3: One hour after daily UV irradiation, apply 0.1 mL of the corresponding anti-aging composition to the shaved area.

[0126] Comparative Examples 1-5: One hour after daily UV exposure, apply 0.1 mL of the corresponding anti-aging composition to the shaved area.

[0127] The ultraviolet irradiation method is as follows: combined UVA and UVB irradiation is used, with a UVA to UVB dose ratio of 9:1. Irradiation is performed once a day. In the first week, the combined ultraviolet irradiation dose is 100 mJ / cm². 2 The dose in week 2 was 200 mJ / cm². 2 The dose in week 3 was 300 mJ / cm. 2 The dose during weeks 4-8 was 400 mJ / cm². 2 .

[0128] (1) Macro score

[0129] 24 hours after applying the corresponding composition in week 8, at least 5 researchers, unaware of the group assignments, blindly scored the irradiated area on the back of the mice under standard light conditions. The scoring criteria are shown in Table 3, and the scoring results are shown in Table 4.

[0130] Table 3 Macro Scoring Criteria

[0131] wrinkle erythema Scales / Desquamation score Skin is smooth and firm, without any visible wrinkles. The skin color was normal, with no difference from the un-irradiated areas, and no signs of redness. The skin surface is free of any scales or dry flakes. 0 The skin is slightly rough, with extremely fine and sparse superficial lines. The skin is light pink with a slight redness. Slightly dry skin with fine powdery flakes 1 Clear but shallow wrinkles are visible; the lines are independent of each other and do not form a network. The skin appears distinctly pink or has localized mild redness. Dry skin with visible fine white scales 2 Wrinkles have deepened significantly, some forming incomplete mesh-like patterns. Skin is sagging. The skin is dark red or the red area is expanding. Extensive skin desquamation, with large, white or grayish-white scales visible on the skin. 3 Deep, coarse wrinkles cover the surface, forming a dense, rough, net-like or grooved pattern, giving it a leather-like appearance. The skin is deep red to purplish-red, with a rich color, and may be accompanied by slight swelling. —— 4

[0132] Table 4. Mouse scores in each group

[0133] Group Wrinkles (divided) Erythema (divided) Scales / Desquamation (classified) Total score (points) Blank control group 0 0 0 0 Model group 4.5 4.4 3.1 12.0 Positive control group 1.6 1.5 1.4 4.5 Example 1 Group 1.0 1.1 1.2 3.3 Example 2 group 1.1 1.4 1.3 3.8 Example 3 Group 1.3 1.2 1.3 3.8 Comparative Example 1 2.1 2.2 2.0 6.3 Comparative Example 2 2.3 2.0 1.8 6.1 Comparative Example 3 1.8 3.7 1.7 7.2 Comparative Example 4 1.9 1.8 1.5 5.2 Comparative Example 5 1.9 1.6 1.6 5.1

[0134] Table 4 shows that the model group had significantly higher scores in wrinkles, erythema, and scaling / desquamation compared to the blank control group, indicating the successful establishment of the photoaging mouse model. The effects of Examples 1-3 were higher than the positive control group, demonstrating the significant anti-photoaging effect of the composition of this invention. A comparison of Example 1 and Comparative Examples 1-2 shows that recombinant human type III collagen and the film-forming agent have a synergistic effect, alleviating skin wrinkles, erythema, and scaling / desquamation symptoms caused by ultraviolet light. A comparison of Example 1 and Comparative Example 3 shows that the erythema score was the highest, indicating that recombinant human type III collagen has lower skin irritation than laurocapram. A comparison of Example 1 and Comparative Examples 4-5 shows that different complex microorganisms and solvents can affect the anti-photoaging effect of the raw material extraction.

[0135] (2) Skin indicators

[0136] After scoring the mice, blood was collected by enucleation into centrifuge tubes, allowed to stand, and then centrifuged (3000 rpm, 10 min) to separate the serum for the determination of malondialdehyde (MDA).

[0137] The mice were then euthanized, and the skin tissue from the shaved area was quickly cut off, rinsed with pre-cooled physiological saline to remove bloodstains and adhering substances, and blotted dry with filter paper. 0.1 g of skin tissue was mixed with 0.9 mL of 4℃ PBS solution (pH 7.4), homogenized at 3000 rpm for 15 s under ice bath conditions, and repeated 4 times to obtain a skin tissue homogenate.

[0138] The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in skin tissue homogenate were detected, and the content of malondialdehyde (MDA) in serum was also measured.

[0139] SOD activity was detected by hydroxylamine method, GSH-Px activity was detected by colorimetry, CAT activity was detected by ammonium molybdate method, and MDA content was detected by thiobarbituric acid method.

[0140] The results of SOD activity, GSH-Px activity, CAT activity in skin tissue, and MDA content in serum are shown in Table 5.

[0141] Table 5. SOD activity, GSH-Px activity, CAT activity, and MDA content in each group of mice.

[0142] Group SOD (U / mL) GSH-Px (U / mL) CAT (U / mL) MDA (nmol / mL) Blank control group 96.23 896.63 11.53 5.15 Model group 52.65 428.27 4.81 14.23 Positive control group 88.91 821.82 10.38 5.81 Example 1 Group 86.08 830.58 10.17 5.69 Example 2 group 88.54 824.06 10.22 5.73 Example 3 Group 88.34 835.91 10.49 5.57 Comparative Example 1 70.39 683.78 7.15 9.30 Comparative Example 2 72.82 692.80 7.30 9.24 Comparative Example 3 68.16 677.14 6.92 9.72 Comparative Example 4 80.70 759.49 8.24 8.08 Comparative Example 5 82.47 765.35 8.56 7.86

[0143] Table 5 shows that, compared with the blank control group, the activity of SOD, GSH-Px, and CAT in the skin tissue of the model group was significantly reduced, indicating that the mouse photoaging model was successfully established. Examples 1-3 were superior to the positive control group, indicating that the composition of the present invention has good anti-photoaging effects. A comparison of Example 1 and Comparative Examples 1-2 shows that recombinant human type III collagen and the film-forming agent have a synergistic effect; the combination of the two promotes the penetration of the active ingredients while controlling the release efficiency, allowing the active ingredients to penetrate efficiently into the skin and improving the anti-photoaging effect. A comparison of Example 1 and Comparative Example 3 shows that a suitable penetration enhancer can improve the anti-photoaging effect of the body. A comparison of Example 1 and Comparative Examples 4-5 shows that the extraction of raw materials using specific composite microorganisms and solvents can affect the anti-photoaging effect.

[0144] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An anti-aging composition for improving the penetration effect of extracts from the bark of the variegated bellflower tree, characterized in that, It is composed of the following raw materials in parts by weight: 0.05-0.2 parts of recombinant human type III collagen, 0.05-0.1 parts of extract from the bark of the variegated bellflower tree, 0.01-0.03 parts of extract from the fruit of the hairy custard apple, 0.03-0.08 parts of extract from the flower of the magnolia officinalis, and 1.5-2.5 parts of film-forming agent; The film-forming agent is composed of rhamnose and a trimethylpentanediol / adipic acid / glycerol cross-linked polymer; The weight ratio of rhamnose to the trimethylpentanediol / adipic acid / glycerol crosslinked polymer is (0.5-1):(1-1.5); The preparation method of the soursop fruit extract is as follows: Fresh whole custard apples were intermittently crushed in a high-speed blender at 20,000-30,000 rpm for 30 seconds, then paused for 30 seconds. This process was repeated 3-4 times to obtain a slurry. 12-17 times the weight of the slurry was added to the slurry, and the mixture was sterilized. Then, a compound microorganism was inoculated and anaerobic fermented at 35-40℃ and pH 6-7 for 56-72 hours. The mixture was then sterilized by UV irradiation, filtered through a 0.22μm membrane, and the filtrate was freeze-dried under vacuum to obtain custard apple fruit extract. The compound microorganisms consisted of Lactobacillus plantarum WCFS1 and Bifidobacterium animalis W3. The preparation method of the Magnolia officinalis flower extract includes: crushing dried Magnolia officinalis flower buds through a 50-100 mesh sieve to obtain Magnolia officinalis pollen; adding a low-eutectic solvent aqueous solution at a ratio of 1:15-20 g / mL; extracting at 60-70℃ and 200-400 rpm for 60-90 min; cooling to 25-30℃; centrifuging at 8000-10000 rpm for 10-15 min to obtain a supernatant; adsorbing the supernatant onto an AB-8 type macroporous adsorption resin column at a flow rate of 1.5-2.5 BV / h; eluting with 3-5 BV of water at a flow rate of 2 BV / h; eluting with 4-6 BV of 68%-72% ethanol solution at a flow rate of 1-2 BV / h; collecting the eluent of 68%-72% ethanol solution; and freeze-drying the eluent under vacuum to a water content of 2wt%-5wt% to obtain the Magnolia officinalis flower extract.

2. The anti-aging composition according to claim 1, characterized in that, The eutectic solvent consists of choline chloride and glycerol.

3. A method for preparing the anti-aging composition according to any one of claims 1-2, characterized in that, include: Recombinant human type III collagen, extracts of *Bretschneidera sinensis* bark, extracts of *Magnolia denudata* flower, extracts of *Annona squamosa* fruit, rhamnose, trimethylpentanediol / adipic acid / glycerol crosspolymer, and water were mixed and homogenized to obtain an anti-aging composition.

4. The preparation method according to claim 3, characterized in that, The homogenization process is carried out at a speed of 3000-4000 rpm for 10-15 minutes.

5. The use of the composition according to any one of claims 1-4 in the preparation of anti-aging and repair products.

6. The application according to claim 5, characterized in that, The aging process is divided into photoaging and natural aging.