Rapid detection device for groundwater pollution based on biosensor array

Abstract

The application discloses a groundwater pollution rapid detection device based on a biosensor array, and relates to the technical field of detection devices.The device comprises a crawling pipeline, a biosensing detection module, a sampling module and a liquid feeding module.The sampling module, the liquid feeding module and the biosensing detection module are independently assembled and are connected through standardized interfaces, which facilitates maintenance and cleaning, and simultaneously solves the problem of independent work and connection faults of traditional split-type equipment modules.The liquid feeding module of the application can automatically feed sample liquid to the detection module, and the whole process is seamlessly connected, thereby avoiding time loss caused by independent work of modules in traditional split-type devices, reducing redundant structures, and reducing the overall volume by arranging the modules along an axis instead of stacking them, which is more suitable for deployment in narrow spaces.

Description

Technical Field

[0001] This invention relates to the field of detection device technology, and in particular to a rapid detection device for groundwater pollution based on a biosensor array. Background Technology

[0002] Groundwater is a vital water resource for maintaining ecological balance and supporting social production and daily life, and its water quality safety is of paramount importance. However, in the process of industrialization and urbanization, illegal discharge of industrial wastewater, excessive use of chemical fertilizers and pesticides in agricultural production, infiltration of domestic sewage, and leakage from landfills have led to a large number of harmful substances, such as heavy metals, persistent organic pollutants, and toxic microorganisms, entering groundwater bodies.

[0003] In the field of groundwater pollution detection, traditional detection equipment has many limitations. Early integrated detection equipment combined sampling, transportation, and detection functions into a single structure. While it could achieve basic detection, cleaning and maintenance were inconvenient. The entire device had to be disassembled to maintain internal pipelines or core components, which not only made operation complex and maintenance costs high, but also made the equipment bulky and difficult to deploy in confined spaces. At the same time, its integrated structure resulted in poor portability, making it impossible to transport or carry in multiple trips, and its applicability in complex environments such as the field was low.

[0004] While traditional split-type testing equipment solves some of the portability issues, it suffers from a lack of standardized interfaces and collaborative control logic, resulting in gaps in module integration. The sampling, sample delivery, and testing modules operate independently, requiring manual transfer of sample solutions. This increases the risk of contamination and leads to low testing efficiency due to the time-consuming manual operation, often requiring several hours for a single test.

[0005] To address these issues, this invention proposes a rapid groundwater pollution detection device based on a biosensor array. Summary of the Invention

[0006] In view of the problems existing in the prior art, the present invention is proposed.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a rapid detection device for groundwater pollution based on a biosensor array, comprising:

[0008] Straight-tube crawling pipe;

[0009] The biosensor detection module located at the top of the crawling pipe has an upper connecting pipe at its bottom.

[0010] The sampling module is located at the bottom of the crawling pipe, and its top is equipped with a lower connecting pipe;

[0011] The liquid delivery module located in the middle of the crawling pipe includes a crawling unit, a liquid extraction unit, and a docking unit. The crawling unit carries the liquid extraction unit and the docking unit, and drives the liquid extraction unit and the docking unit to crawl along the axis of the crawling pipe to dock with the sampling module or the biosensor detection module in sequence. When the docking unit docks with the sampling module, its liquid extraction unit draws and stores the sample liquid through the sampling module. When the docking unit docks with the biosensor detection module, the liquid extraction unit sends the temporarily stored sample liquid to the biosensor detection module for rapid detection.

[0012] As a preferred embodiment of the rapid detection device for groundwater pollution based on a biosensor array according to the present invention, the crawling unit includes a frame body located in the middle, multiple sets of mounting base plates arranged around the frame body, multiple horizontal support arms connected to the mounting base plates, and walking components arranged on the horizontal support arms. The walking components supported by the multiple sets of horizontal support arms are arranged parallel to the central axis of the frame body.

[0013] As a preferred embodiment of the rapid detection device for groundwater pollution based on a biosensor array according to the present invention, the number of docking units is two sets and they are symmetrically arranged on both sides of the frame body. The frame body includes a connecting base threaded to the frame body and a rigid pipe vertically assembled on the connecting base. One end of the rigid pipe located in the inner cavity of the frame body is machined to form an external thread pipe end, and a pressure sensor is provided on the docking surface of the other end of the rigid pipe.

[0014] As a preferred embodiment of the rapid detection device for groundwater pollution based on a biosensor array according to the present invention, the pumping unit includes a support base plate assembled on the inner wall of the frame, a gear pump arranged on the support base plate, and a hose passing through the inner cavity of the gear pump. Both ends of the hose are equipped with pipe joints, and the hose is threadedly connected to a rigid pipe through the pipe joints.

[0015] Both ends of the hose that extend from the gear pump are folded and arranged within the internal cavity of the frame, forming a chamber for temporary storage of the sample solution inside the hose.

[0016] As a preferred embodiment of the rapid groundwater pollution detection device based on a biosensor array according to the present invention, the sampling module includes a sampling tube with a two-piece symmetrical structure, a double-layer mounting base disposed inside the sampling tube, and an arc-shaped clamping plate disposed at the bottom of the outer wall of each sampling tube; the lower connecting pipe is supported and limited by the double-layer mounting base, the lower connecting pipe extends outward through the constricted end of the sampling tube, the side of the lower connecting pipe opposite to the constricted end of the sampling tube is higher than the bottom end of the sampling tube, and the mating surface of its port is covered with a sealing silicone strip.

[0017] As a preferred embodiment of the rapid groundwater pollution detection device based on a biosensor array according to the present invention, a pretreatment module is provided at the constricted end of the sampling tube. The pretreatment module includes a split pretreatment tube and a sieve screen snapped into the split pretreatment tube. A snap-fit ​​connector is fitted to the end of the split pretreatment tube facing the sampling tube. The split pretreatment tube is snapped into an arc-shaped clamping plate through the snap-fit ​​connector.

[0018] As a preferred embodiment of the rapid groundwater pollution detection device based on a biosensor array according to the present invention, the sampling tube is provided with a fastening component on its exterior. The fastening component includes an interleaved distribution of elastic rubber strips and annular airbags. The inner diameter of the elastic rubber strips and annular airbags under natural contraction is smaller than the outer diameter of the sampling tube, and the outer diameter of the elastic rubber strips and annular airbags under natural contraction is larger than the inner diameter of the outer diameter crawling pipe.

[0019] As a preferred embodiment of the rapid groundwater pollution detection device based on a biosensor array according to the present invention, the biosensor detection module includes a protective cylinder, a stabilizing component arranged outside the protective cylinder, a signal conversion module, a signal transmission module, a real-time detection module, and a pipe mounting plate arranged from top to bottom inside the protective cylinder, the upper connecting pipe being vertically inserted into the center of the pipe mounting plate, and the upper connecting pipe penetrating one end of the pipe mounting plate and connecting to the real-time detection module.

[0020] As a preferred embodiment of the rapid groundwater pollution detection device based on a biosensor array according to the present invention, the real-time detection module includes a base box and a biosensor array arranged on the bottom surface of the base box. The biosensor array includes multiple independent sensing units. The port of the upper connecting pipe is located at the center of the base box and is flush with the bottom surface of the base box. Multiple connecting grooves extend outward from the port of the upper connecting pipe. The multiple connecting grooves are used to transport the drug solution in the upper connecting pipe to the multiple independent sensing units.

[0021] The bottom surface of the base box is inclined, sloping downwards from the center of the base box to the edge.

[0022] As a preferred embodiment of the rapid groundwater pollution detection device based on a biosensor array according to the present invention, the independent sensing unit includes a detection cavity opened on the bottom surface of the base box and a sensitive layer arranged inside the detection cavity;

[0023] The signal transmission module includes a needle suspension plate and multiple signal transmission needles vertically inserted into the needle suspension plate. The signal transmission needles are connected to the sensitive layer inside the detection cavity.

[0024] The beneficial effects of this invention are as follows: In this application, the sampling module, liquid delivery module, and biosensor detection module are independently assembled and connected through standardized interfaces, which facilitates maintenance and cleaning. At the same time, it solves the problem of independent operation and disconnection of modules in traditional split-type devices. The liquid delivery module of this application can automatically deliver the sample liquid to the biosensor detection module. The whole process is seamlessly connected, avoiding the time loss caused by the independent operation of modules in traditional split-type devices. At the same time, the modular design reduces redundant structures. The crawling pipe serves as a common carrier, and the modules are distributed along the axis rather than stacked, reducing the overall volume and making it more suitable for deployment in narrow spaces. Attached Figure Description

[0025] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the overall structure of the rapid groundwater pollution detection device based on a biosensor array in this invention.

[0027] Figure 2 A top view of the overall structure of the rapid groundwater pollution detection device based on a biosensor array in this invention;

[0028] Figure 3 This is an isometric view of the overall structure of the rapid groundwater pollution detection device based on a biosensor array in this invention.

[0029] Figure 4 For the present invention Figure 3 Enlarged view of the A-section structure;

[0030] Figure 5 This is a schematic diagram of the internal structure of the frame in this invention;

[0031] Figure 6 This is a schematic diagram of the overall structure of the sampling module in this invention;

[0032] Figure 7 This is a schematic diagram of the overall structure of the biosensing detection module in this invention;

[0033] Figure 8 For the present invention Figure 7 Enlarged view of the structure of part B.

[0034] Reference numerals: 01. Crawling pipe; 02. Biosensor detection module; 021. Protective casing; 022. Upper connecting pipe; 023. Stabilizing component; 024. Pipe mounting plate; 025. Signal conversion module; 026. Signal transmission module; 0261. Needle suspension plate; 0262. Signal transmission needle; 027. Real-time detection module; 0271. Base box; 0272. Connecting groove; 0273. Detection chamber; 0274. Sensitive layer; 03. Liquid delivery module; 031. Docking unit; 0311. Hoses; 0312. Support base plate; 0313. Gear pump; 0314. Pipe joint; 032. Crawling unit; 0321. Frame body; 0 322. Mounting base plate; 0323. Horizontal support arm; 0324. Wheel system support; 0325. Track body; 0326. Drive roller; 0327. Base plate; 0328. Connecting pair; 0329. Rectangular window; 033. Liquid extraction unit; 0331. Connecting base; 0332. Rigid pipe; 034. Drive component; 04. Sampling module; 041. Sampling cylinder; 042. Lower connecting pipe; 043. Double-layer mounting base; 044. Sealing silicone strip; 045. Elastic rubber strip; 046. Annular airbag; 047. Arc-shaped clamping plate; 05. Pretreatment module; 051. Split-type pretreatment cylinder; 052. Screening mesh; 053. Clamping connector. Detailed Implementation

[0035] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0036] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0037] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places throughout this specification does not necessarily refer to the same embodiment, nor is it a single embodiment or an embodiment selectively excluded from other embodiments.

[0038] Reference Figures 1-8 As shown, a rapid detection device for groundwater pollution based on a biosensor array includes...

[0039] Straight-tube crawler pipe 01;

[0040] The biosensor detection module 02 located at the top of the crawling pipe 01 has an upper connecting pipe 022 at its bottom.

[0041] The sampling module 04 is located at the bottom of the crawling pipe 01, and its top is provided with a lower connecting pipe 042;

[0042] The liquid delivery module 03, located in the middle of the crawling pipe 01, includes a crawling unit 032, a liquid extraction unit 033, and a docking unit 031. The crawling unit 032 carries the liquid extraction unit 033 and the docking unit 031, and drives the liquid extraction unit 033 and the docking unit 031 to crawl along the axis of the crawling pipe 01 to dock with the sampling module 04 or the biosensor detection module 02 in turn. When the docking unit 031 docks with the sampling module 04, its liquid extraction unit 033 draws and stores the sample liquid through the sampling module 04. When the docking unit 031 docks with the biosensor detection module 02, the liquid extraction unit 033 sends the temporarily stored sample liquid to the biosensor detection module 02 for rapid detection. The sample liquid mentioned above is groundwater.

[0043] like Figures 1-5 As shown, the crawling unit 032 includes a frame body 0321 located in the middle, multiple sets of mounting base plates 0322 arranged around the frame body 0321, multiple horizontal support arms 0323 connected to the mounting base plates 0322, and walking components arranged on the horizontal support arms 0323. The walking components supported by the multiple sets of horizontal support arms 0323 are arranged parallel to the central axis of the frame body 0321.

[0044] Furthermore, the frame body 0321 has large openings at both ends, and a spiral keyway is provided at the large openings of the frame body 0321. The frame body 0321 has multiple rectangular windows 0329 on its side, and the multiple rectangular windows 0329 and multiple sets of mounting base plates 0322 are staggered together.

[0045] Furthermore, multiple sets of mounting base plates 0322 are arranged at equal intervals on the side wall of the frame body 0321. A base plate 0327 is provided between the mounting base plates 0322 and the frame body 0321. The mounting base plates 0322 are connected to the frame body 0321 through the base plate 0327. The two ends of the horizontal support arm 0323 are connected to the mounting base plates 0322 and the traveling component, respectively. A connecting pair 0328 is provided at the connection point. The connecting pair 0328 is configured with matching bolts and nuts.

[0046] Furthermore, the traveling component includes a wheel system support 0324 connected to the horizontal support arm 0323, two sets of transmission rollers 0326 disposed within the wheel system support 0324, and a track body 0325 driven by the two sets of transmission rollers 0326. The transmission rollers 0326 and the track body 0325 mesh with each other. One end of one set of transmission rollers 0326 protruding from the wheel system support 0324 is connected to a drive component 034. The drive component 034 is mounted on the surface of the wheel system support 0324 and is equipped with a rotary motor. The track body 0325 is guided by the wheel system support 0324 to form a specific shape. The output shaft of the rotary motor drives the transmission rollers 0326 to drive the track body 0325 meshing with the transmission rollers 0326 to rotate along the surface of the wheel system support 0324, while simultaneously driving the other set of transmission rollers 0326 to rotate.

[0047] Furthermore, the drive unit 034 also includes a receiver disposed within the rotary motor, which can remotely receive signals to control the rotary motor switch; when the track body 0325 rotates along the surface of the wheel support 0324, its entire crawling unit 032 carries the liquid pumping unit 033 and docking unit 031 to move within the pipeline.

[0048] The number of docking units 031 is two sets and they are symmetrically arranged on both sides of the frame body 0321. The frame body 0321 includes a connecting base 0331 threadedly connected to the large opening of the frame body 0321 and a rigid pipe 0332 vertically mounted on the connecting base 0331. One end of the rigid pipe 0332 located in the inner cavity of the frame body 0321 is machined to form an external thread pipe end, and a pressure sensor is provided on the docking surface of the other end of the rigid pipe 0332.

[0049] The liquid extraction unit 033 includes a support base plate 0312 mounted on the inner wall of the frame body 0321, a gear pump 0313 arranged on the support base plate 0312, and a hose 0311 passing through the inner cavity of the gear pump 0313. Both ends of the hose 0311 are equipped with pipe joints 0314, and the hose 0311 is threadedly connected to the rigid pipe 0332 through the pipe joints 0314.

[0050] Both ends of the tubing 0311 that protrude from the gear pump 0313 are folded and arranged inside the frame body 0321, forming a chamber for temporary storage of sample liquid inside the tubing 0311.

[0051] Furthermore, a silicone pad is provided inside the pipe joint 0314, and the pipe joint 0314 is tightly connected to the rigid pipe 0332 and the flexible hose 0311 respectively through the silicone pad.

[0052] like Figure 6As shown, the sampling module 04 includes a sampling tube 041 with a two-piece symmetrical structure, a double-layer mounting base 043 disposed inside the sampling tube 041, and an arc-shaped clamping plate 047 disposed at the bottom of the outer wall of each sampling tube 041; the lower connecting pipe 042 is supported and limited by the double-layer mounting base 043, the lower connecting pipe 042 extends outward through the constricted end of the sampling tube 041, the side of the lower connecting pipe 042 away from the constricted end of the sampling tube 041 is higher than the bottom end of the sampling tube 041, and the mating surface of its port is covered with a sealing silicone strip 044.

[0053] A pretreatment module 05 is provided at the constricted end of the sampling cylinder 041. The pretreatment module 05 includes a split pretreatment cylinder 051 and a sieve 052 snapped into the split pretreatment cylinder 051. A snap-fit ​​connector 053 is installed at the end of the split pretreatment cylinder 051 facing the sampling cylinder 041. The split pretreatment cylinder 051 is snapped onto the arc-shaped clamping plate 047 through the snap-fit ​​connector 053.

[0054] Furthermore, the sampling tube 041 is provided with fastening components on its exterior. The fastening components include interleaved elastic rubber strips 045 and annular air bladders 046. The inner diameter of the elastic rubber strips 045 and annular air bladders 046 under natural contraction is smaller than the outer diameter of the sampling tube 041, and the outer diameter of the elastic rubber strips 045 and annular air bladders 046 under natural contraction is larger than the inner diameter of the outer diameter crawling pipe 01.

[0055] Furthermore, an adapter groove is provided on the outer wall of the sampling tube 041 for engaging the elastic rubber strip 045 and the annular airbag 046.

[0056] like Figures 7-8 As shown, the biosensor detection module 02 includes a protective cylinder 021, a stabilizing component 023 arranged outside the protective cylinder 021, a signal conversion module 025, a signal transmission module 026, a real-time detection module 027 arranged inside the protective cylinder 021 from top to bottom, and a pipe mounting plate 024. An upper connecting pipe 022 is vertically inserted into the center of the pipe mounting plate 024, and one end of the upper connecting pipe 022 that penetrates through the pipe mounting plate 024 is connected to the real-time detection module 027.

[0057] Furthermore, the stabilizing component 023 is constructed in the same way as the fastening component, and is used to install the biosensing detection module 02 inside the crawling pipe 01.

[0058] The real-time detection module 027 includes a base box 0271 and a biosensor array arranged on the bottom surface of the base box 0271. The biosensor array includes multiple independent sensing units. The port of the upper connecting pipe 022 is located at the center of the base box 0271 and is flush with the bottom surface of the base box 0271. Multiple connecting grooves 0272 extend outward from the port of the upper connecting pipe 022. The multiple connecting grooves 0272 are used to transport the drug solution in the upper connecting pipe 022 to the multiple independent sensing units.

[0059] The bottom surface of the base box 0271 is inclined, sloping downward from the center of the base box 0271 to the edge.

[0060] Furthermore, the independent sensing unit includes a detection cavity 0273 formed on the bottom surface of the base box 0271 and a sensitive layer 0274 arranged inside the detection cavity 0273. The sensitive layer 0274 is a biometric material. Contaminants will combine with it to form a complex, which will cause changes in the physical properties of the sensitive layer 0274, thereby changing the conductivity and dielectric constant of the sensitive layer 0274, which will in turn cause the sensitive layer 0274 to generate a weak electrical signal, and thus generate changes in current and voltage.

[0061] The signal transmission module 026 includes a needle suspension plate 0261 and a plurality of signal transmission needles 0262 vertically inserted on the needle suspension plate 0261. The signal transmission needles 0262 are connected to the sensitive layer 0274 inside the detection cavity 0273.

[0062] Furthermore, the signal conversion module 025 has a built-in amplifier circuit and filter chip, which is used to receive the signal transmitted by the signal transmission pin 0262. After amplifying and reducing the noise of the received signal, it is transmitted to the signal conversion module 025 through the wire.

[0063] Furthermore, the signal conversion module 025 is used to convert the conditioned electrical signal into a digital signal, and calculate the type and concentration of pollutants in the sample solution by combining it with a pre-set calibration curve, and finally send it to an external terminal to realize rapid detection of groundwater pollution; wherein the calibration curve is a curve generated by different pollutant concentrations corresponding to specific signal intensities.

[0064] Working principle: Before entering the formal sampling and testing process, the groundwater pollution rapid detection device must first complete the module installation and initial positioning of the liquid delivery module 03 to ensure that each component is in the preset working position.

[0065] First, the sampling module 04 is deployed; the elastic rubber strip 045 and the annular airbag 046 are sleeved on the outer wall of the sampling cylinder 041 in an alternating manner, and the elastic rubber strip 045 and the annular airbag 046 are set in the corresponding adapter slots so that the elastic rubber strip 045 and the annular airbag 046 are not easy to slide along the cylinder body after being sleeved on the outer wall of the sampling cylinder 041.

[0066] Secondly, the elastic rubber strip 045 and the annular airbag 046 are sleeved on the outer wall of the sampling tube 041, thereby generating a fastening force in the sampling tube 041 so that the two-piece symmetrical sampling tube 041 forms a complete tube body, which has a constricted end and a flared end.

[0067] The sieve 052 is embedded into the inner cavity of the split pretreatment cylinder 051. The protrusions on the edge of the sieve 052 engage and fix with the grooves on the inner wall of the split pretreatment cylinder 051 to prevent displacement due to water flow impact during filtration. Then, the end of the split pretreatment cylinder 051 facing the sampling cylinder 041 is aligned with the arc-shaped retaining plate 047 at the constricted end of the sampling cylinder 041 so that the retaining connector 053 is fully embedded in the surface of the arc-shaped retaining plate 047. The pretreatment module 05 and the sampling cylinder 041 form a stable connection, and the sieve 052 is exactly at the front end of the water inlet of the sampling cylinder 041, preparing for sample filtration.

[0068] Then, the sampling module 04 is installed at the end of the crawling pipe 01. The end of the crawling pipe 01 facing the position to be measured is the lower installation end. During installation, the sampling tube 041 along with the pretreatment module 05 needs to be slowly pushed into the pipe from the lower installation end. Since the outer diameters of the elastic rubber strip 045 and the annular airbag 046 in their naturally contracted state are larger than the inner diameter of the crawling pipe 01, when the sampling tube 041 enters the pipe, both will be deformed by the inner wall of the pipe. The elastic rubber strip 045 will bend and contract inward, and the annular airbag 046 will be compressed to fit against the outer wall of the sampling tube 041. The reaction force generated by the deformation will make the sampling tube 041 fit tightly against the inner wall of the pipe. It should be noted that the groundwater needs to overflow the constricted end of the sampling tube 041 and the screen 052 will be directly exposed to the water source to be tested, providing a filtration barrier for the subsequent sample liquid to enter the sampling tube 041. The main body of the sampling tube 041 is located inside the pipe, and the lower connecting pipe 042 at its top extends upward out of the inner cavity of the sampling tube 041, reserving sufficient docking space for the docking unit 031 of the liquid delivery module 03.

[0069] Place the liquid delivery module 03 into the crawling pipe 01 and turn on the rotary motor to drive the liquid delivery module 03 to the middle of the sampling pipe.

[0070] Insert the liquid delivery module 03 into the crawling pipe 01 from the end furthest from the sampling module 04, ensuring the axis of the frame 0321 is aligned with the pipe axis during pushing. At this time, the track body 0325 of the walking component contacts the inner wall of the pipe. Due to the compression from the crawling pipe 01, the track body 0325 undergoes slight deformation, tightly fitting against the inner wall of the pipe. Activate the rotary motor to push the liquid delivery module 03 to the middle position of the crawling pipe 01.

[0071] The installation of the assembly and testing module relies on the deformation of the stabilizing structure. The stabilizing structure is deformed by the inner wall of the crawling pipe 01, generating radial pressure to fix the module to the upper part of the pipe. During the pushing process, it is necessary to observe the position of the upper docking pipe 022 to ensure that it is aligned with the rigid pipe 0332 of the docking unit 031.

[0072] First, the operator sends a sampling command to the receiver of the drive component 034 via a remote control terminal. The rotary motor starts and drives the track body 0325 to rotate forward, causing the liquid delivery module 03 to move downward along the pipeline axis. During the movement, the frame body 0321 stably supports the docking unit 031 through the horizontal arm, ensuring that the rigid pipeline 0332 is accurately aligned with the lower connecting pipe 042 of the sampling module 04. When the docking surface of the rigid pipeline 0332 contacts the lower connecting pipe 042, the pressure sensor detects the pressure signal and feeds it back to the control terminal. The motor stops running, and at this time, the sealing silicone strip 044 of the lower connecting pipe 042 and the contact surface of the rigid pipeline 0332 form a sealed space to prevent sample leakage.

[0073] Gear pump 0313 starts operating, generating negative pressure through the passage between hose 0311 and rigid pipe 0332, drawing groundwater from sampling tube 041 into the pipeline. The sample solution first passes through screen 052 to filter out micro-impurities, then flows into hose 0311 along the lower connecting pipe 042 and rigid pipe 0332. Because the ends of hose 0311 are folded to form temporary storage chambers, a relatively long cavity is formed to accommodate the sample solution. Under the continuous suction of gear pump 0313, the sample solution gradually fills the cavity until a certain volume is reached. The operator predicts the volume based on the operating time of gear pump 0313. After extraction is complete, gear pump 0313 stops operating. The gear structure built into gear pump 0313 naturally compresses the middle of hose 0311, causing hose 0311 to close at that position. Even if the liquid delivery module 03 tilts or vibrates during its movement, the sample liquid is not easy to flow out due to the blockage of the central passage under the action of gravity, thus achieving stable temporary storage of the sample liquid; then the rotary motor reverses its rotation, the liquid delivery module 03 moves upward and disengages from the sampling module 04, and returns to the middle of the pipeline to wait.

[0074] The operator sends a sample delivery command to the receiver of the drive component 034 via a remote control terminal. The rotary motor drives the track body 0325 to continue moving upward until the rigid pipe 0332 of the docking unit 031 aligns with the upper docking pipe 022 of the biosensor detection module 02. After confirming a tight seal via feedback signal from the pressure sensor, the gear pump 0313 reverses its rotation, pumping out the temporary sample solution from the chamber of the hose 0311. The sample solution flows along the rigid pipe 0332 and the upper docking pipe 022 into the base box 0271 of the biosensor detection module 02. Because the bottom surface of the base box 0271 slopes from the center to the edge, the sample solution naturally diffuses along the connecting groove 0272 under the action of gravity, evenly distributing to each detection chamber 0273, ensuring that each independent sensing unit can contact the sample solution. Then, the liquid delivery module 03 moves downward to disengage from the docking and returns to the middle of the sampling tube, completing the liquid delivery process.

[0075] When the sample solution in the substrate chamber 0271 is delivered to each detection chamber 0273, the contaminants therein specifically bind to the sensitive layer 0274, which causes significant fluctuations in the conductivity and dielectric constant of the sensitive layer 0274, resulting in weak changes in current and voltage. The higher the concentration of contaminants, the greater the fluctuation amplitude of the electrical signal.

[0076] The signal transmission needle 0262 of the signal transmission module 026 captures changes in current and voltage in real time to form an electrical signal. The needle tip is in close contact with the sensitive layer 0274, and the weak electrical signal is led out to the needle body suspension plate 0261, and then transmitted to the signal conversion module 025 through the wire.

[0077] After receiving the raw electrical signal, the signal conversion module 025 converts the data and performs error calibration simultaneously to form a real-time monitoring curve. The real-time monitoring curve is compared with the calibration curve to calculate the type and concentration of pollutants in the sample solution, and finally sent to the external terminal.

[0078] Compared to traditional integrated devices that combine sampling, transportation, and detection functions into a single structure, this device adopts a modular design. The sampling module 04, liquid delivery module 03, and biosensor detection module 02 are independently assembled and connected via standardized interfaces. When cleaning or maintenance is required, each module can be disassembled individually without disassembling the entire device, reducing maintenance costs. Furthermore, the rapid detection device features a split structure, allowing for individual assembly and transport in multiple trips, thus improving the portability of the biosensor array-based rapid groundwater pollution detection device. Moreover, the standardized interface and pre-set collaborative control logic address the issue of "independent module operation and disconnected connections" common in traditional split-type devices. Traditional split-type devices often lack a unified docking standard for their modules. The sample liquid collected by the sampling module 04 needs to be manually transferred to the detection module, which not only increases the risk of sample liquid contamination but also prolongs the overall working time due to the time-consuming manual operation. In contrast, the sampling module 04, liquid delivery module 03, and biosensor detection module 02 of this device achieve automated connection of "sampling-sample delivery-detection" through precise docking of the rigid pipe 0332 with the connecting pipe and real-time feedback from the pressure sensor. The liquid delivery module 03 can deliver the sample liquid to the detection module. The whole process is seamlessly connected, avoiding the time loss caused by the independent operation of modules in traditional split-type devices.

[0079] Meanwhile, the modular design reduces redundant structures. The crawling pipe 01 serves as a shared carrier, with each module distributed along the axis rather than stacked, resulting in a smaller overall volume and making it more suitable for deployment in narrow spaces.

[0080] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection", and "linkage" should be interpreted broadly, and can be mechanical or electrical connection, or internal connection between two components, or direct connection. "Up", "down", "left", "right", etc. are only used to indicate relative positional relationship. When the absolute position of the described object changes, the relative positional relationship may change.

[0081] Secondly: The accompanying drawings of the embodiments disclosed in this invention only involve the structures involved in the embodiments disclosed in this invention. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this invention can be combined with each other.

[0082] In conclusion, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A rapid detection device for groundwater pollution based on a biosensor array, characterized in that, include: Straight-tube crawling pipe (01); The biosensor detection module (02) located at the top of the crawling pipe (01) has an upper connecting pipe (022) at its bottom. The sampling module (04) located at the bottom of the crawling pipe (01) has a lower connecting pipe (042) on its top. The liquid delivery module (03) located in the middle of the crawling pipe (01) includes a crawling unit (032), a liquid extraction unit (033), and a docking unit (031). The crawling unit (032) carries the liquid extraction unit (033) and the docking unit (031) and drives the liquid extraction unit (033) and the docking unit (031) to crawl along the axis of the crawling pipe (01) to dock with the sampling module (04) or the biosensor detection module (02) in turn. When the docking unit (031) docks with the sampling module (04), its liquid extraction unit (033) draws and stores the sample liquid through the sampling module (04). When the docking unit (031) docks with the biosensor detection module (02), the liquid extraction unit (033) sends the temporarily stored sample liquid to the biosensor detection module (02) for rapid detection. The crawling unit (032) includes a frame (0321) located in the middle. The number of docking units (031) is two sets, symmetrically arranged on both sides of the frame body (0321). The frame body (0321) includes a connecting base (0331) threadedly connected to the frame body (0321) and a rigid pipe (0332) vertically mounted on the connecting base (0331). One end of the rigid pipe (0332) located in the inner cavity of the frame body (0321) is machined into an externally threaded pipe end, and a pressure sensor is provided on the docking surface of the other end of the rigid pipe (0332). The liquid extraction unit (033) includes a component mounted on the frame body (0321). The inner wall of the frame (0321) includes a support base plate (0312), a gear pump (0313) arranged on the support base plate (0312), and a hose (0311) that passes through the inner cavity of the gear pump (0313). Both ends of the hose (0311) are equipped with pipe joints (0314). The hose (0311) is threadedly connected to the rigid pipe (0332) through the pipe joints (0314). Both ends of the hose (0311) that pass through the gear pump (0313) are folded and arranged in the inner cavity of the frame body (0321). A chamber for temporary storage of sample liquid is formed inside the hose (0311). The sampling module (04) includes a sampling tube (041) with a two-piece symmetrical structure, a double-layer mounting base (043) disposed inside the sampling tube (041), and an arc-shaped clamping plate (047) disposed at the bottom of the outer wall of each sampling tube (041). The lower connecting pipe (042) is supported and limited by the double-layer mounting base (043). The lower connecting pipe (042) extends outward through the constricted end of the sampling tube (041). The side of the lower connecting pipe (042) away from the constricted end of the sampling tube (041) is higher than the bottom end of the sampling tube (041), and the mating surface of its port is covered with a sealing silicone strip (044).

2. The rapid detection device for groundwater pollution based on a biosensor array as described in claim 1, characterized in that: The crawling unit (032) further includes multiple sets of mounting base plates (0322) arranged around the frame body (0321), multiple horizontal support arms (0323) connected to the mounting base plates (0322), and walking components arranged on the horizontal support arms (0323). The walking components supported by the multiple sets of horizontal support arms (0323) are arranged parallel to the central axis of the frame body (0321).

3. The rapid detection device for groundwater pollution based on a biosensor array as described in claim 2, characterized in that: A pretreatment module (05) is provided at the constricted end of the sampling tube (041). The pretreatment module (05) includes a split pretreatment tube (051) and a sieve (052) snapped into the split pretreatment tube (051). A snap-fit ​​connector (053) is installed at one end of the split pretreatment tube (051) facing the sampling tube (041). The split pretreatment tube (051) is snapped into the arc-shaped clamping plate (047) through the snap-fit ​​connector (053).

4. The rapid detection device for groundwater pollution based on a biosensor array as described in claim 3, characterized in that: The sampling tube (041) is provided with a fastening component on its exterior. The fastening component includes an interlaced distribution of elastic rubber strips (045) and an annular airbag (046). The inner diameter of the elastic rubber strips (045) and the annular airbag (046) under natural contraction is smaller than the outer diameter of the sampling tube (041). The outer diameter of the elastic rubber strips (045) and the annular airbag (046) under natural contraction is larger than the inner diameter of the outer diameter crawling pipe (01).

5. The rapid detection device for groundwater pollution based on a biosensor array as described in claim 4, characterized in that: The biosensor detection module (02) includes a protective cylinder (021), a stabilizing component (023) arranged outside the protective cylinder (021), a signal conversion module (025), a signal transmission module (026), a real-time detection module (027), and a pipe mounting plate (024) arranged from top to bottom inside the protective cylinder (021). The upper connecting pipe (022) is vertically inserted into the center of the pipe mounting plate (024), and one end of the upper connecting pipe (022) penetrates the pipe mounting plate (024) and connects with the real-time detection module (027).

6. The rapid detection device for groundwater pollution based on a biosensor array as described in claim 5, characterized in that: The real-time detection module (027) includes a base box (0271) and a biosensor array arranged on the bottom surface of the base box (0271). The biosensor array includes multiple independent sensing units. The port of the upper connecting pipe (022) is located at the center of the base box (0271) and is flush with the bottom surface of the base box (0271). Multiple connecting grooves (0272) extend outward from the port of the upper connecting pipe (022). The multiple connecting grooves (0272) are used to transport the drug solution in the upper connecting pipe (022) to the multiple independent sensing units.

7. The rapid detection device for groundwater pollution based on a biosensor array as described in claim 6, characterized in that: The independent sensing unit includes a detection cavity (0273) formed on the bottom surface of the base box (0271) and a sensitive layer (0274) arranged inside the detection cavity (0273). The signal transmission module (026) includes a needle suspension plate (0261) and a plurality of signal transmission needles (0262) vertically inserted on the needle suspension plate (0261). The signal transmission needles (0262) are connected to the sensitive layer (0274) inside the detection cavity (0273).

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