Coffee germplasm cold resistance simulation test device

Abstract

The invention relates to the technical field of plant cold resistance testing, in particular to a coffee germplasm cold resistance simulation testing device which comprises an outer cylinder, a semicircular cylinder is arranged in the outer cylinder, a detachable cultivation tray is arranged in the semicircular cylinder in a sliding mode, a protruding part located over the cultivation tray is arranged on the outer cylinder, and a refrigerator is arranged on one side of the outer cylinder; the refrigerator comprises a sleeve arranged on one side of the outer cylinder, a guide cylinder is arranged in the sleeve, the guide cylinder and the sleeve rotate to form an annular cavity, and a refrigerating pipe is arranged in the annular cavity through a supporting plate. The fan blows air flow to circulate in the annular cavity and the guide cylinder, the air flow blows on the spiral section of the refrigeration pipe, the cooled refrigeration liquid releases cold when flowing through the zigzag section, it is ensured that the cold resistance difference of different coffee germplasm under the unified low-temperature stress condition can be accurately captured, and the repeatability and credibility of test data are high.

Description

Technical Field

[0001] This application relates to the technical field of plant cold resistance testing, and in particular to a device for simulating the cold resistance testing of coffee germplasm. Background Technology

[0002] As a typical tropical and subtropical economic crop, the cold resistance of coffee germplasm resources directly affects the resilience of the coffee industry as its planting areas expand. Against the backdrop of intensifying global climate fluctuations, low-temperature freezing damage has become a key factor restricting the expansion of coffee cultivation and the stability of yields—ranging from mild yellowing of leaves and hindered photosynthesis to severe frost damage to branches, root necrosis, and even the death of the entire plant. Therefore, screening and cultivating coffee germplasm with strong cold resistance has become an important topic in the field of coffee breeding, and accurately assessing the cold resistance level of different coffee germplasm is the prerequisite and foundation for carrying out this work.

[0003] Traditional assessments of coffee cold resistance rely heavily on observations of natural low-temperature environments in the field. This approach is not only limited by natural conditions such as region, season, and climate, but also suffers from long testing cycles, poor repeatability, and difficulty in accurately controlling the intensity, duration, and associated environmental parameters of low-temperature stress. Consequently, the scientific validity and comparability of the assessment results are insufficient.

[0004] For example, application number CN202310516736.3 describes a testing device for the cold resistance performance of plant branches, relating to the field of plant cold resistance testing technology. This prior art includes an open bracket and a sample placement row. The sample placement row is placed on a groove in the open bracket and fixed by a wire support and anti-side slider fixed to the bottom of the sample placement row. A semiconductor cooling chip is installed inside the sample placement row, placed in the cavity between the upper pressure plate and the placement plate, positioned between square rounded corner holes in the upper pressure plate and the placement plate, and fixed by a limiting strip and a supporting wall. This device can effectively improve the working efficiency and accuracy of plant branch cold resistance testing, easily fix and place the plant branch samples to be tested, prevent the heat generated by the branches from dissipating easily, and simplify the testing operation.

[0005] However, the aforementioned existing technologies still have some shortcomings when testing the cold resistance of coffee varieties:

[0006] The aforementioned existing technologies rely on the compressor refrigeration principle of external low-temperature equipment to achieve cooling. Cooling is achieved through heat exchange between the evaporator and the internal space of the external low-temperature chamber, absorbing heat from the air inside and lowering the temperature. Simultaneously, the refrigerant evaporates and returns to the compressor to complete the cycle. While this allows for a relatively wide temperature control range, it has significant shortcomings in humidity control, making it difficult to adapt to the sensitive temperature and humidity requirements of coffee varieties. Coffee originated in tropical and subtropical regions, and its physiological state and cold resistance response are highly dependent on specific temperature and humidity conditions. Under natural low-temperature stress, coffee-growing areas are often accompanied by high humidity (such as cold waves accompanied by rainfall) or periods of low humidity (such as dry and cold winters). These humidity changes directly affect the degree of frost damage to coffee branches (e.g., high humidity easily leads to leaf freezing, while low humidity may exacerbate branch dehydration).

[0007] As a key factor influencing coffee growth and stress resistance, the cold resistance of plant branches is generally overlooked in existing plant branch cold resistance testing devices, and this deficiency has a particularly significant impact on the accuracy of coffee germplasm cold resistance testing. Under low-temperature conditions, changes in light duration and intensity can directly alter coffee's cold resistance strategy by regulating photosynthetic efficiency, the accumulation of cold-resistant substances (such as proline and soluble sugars), and hormone levels (such as abscisic acid content). For example, short-day conditions may trigger coffee's cold stress mechanism, prompting it to accumulate more protective substances to enhance its cold resistance, while under long-day or low-light conditions, coffee's cold resistance response may exhibit completely different characteristics.

[0008] Based on this, and given the above viewpoints, there is still room for improvement in the existing methods for testing the cold resistance of coffee varieties. Summary of the Invention

[0009] To address the aforementioned technical problems, this application provides a coffee germplasm cold resistance simulation testing device, employing the following technical solution:

[0010] A cold resistance simulation testing device for coffee germplasm includes an outer cylinder, a semi-cylinder inside the outer cylinder, a detachable cultivation tray slidably disposed inside the semi-cylinder, a protrusion located directly above the cultivation tray on the outer cylinder, and a cooler disposed on one side of the outer cylinder.

[0011] The refrigerator includes a sleeve on one side of the outer cylinder, and multiple circumferentially evenly distributed support plates inside the sleeve. One end of the multiple support plates is connected to a guide tube, which forms an annular cavity with the sleeve. A refrigeration pipe is installed in the annular cavity through the support plates. Both ends of the refrigeration pipe pass through the protrusion and are interconnected to form a circuit.

[0012] Preferably, the refrigeration pipe is provided with a spiral section located in the annular cavity and a tortuous section located in the protrusion.

[0013] Preferably, an air collecting hood is provided at one end of the guide tube, and an air inlet pipe is connected to the air collecting hood;

[0014] One end of the air inlet pipe passes through the protrusion and is then fitted onto the outside of the refrigeration pipe.

[0015] Preferably, the air inlet pipe is provided with a bend section corresponding to the bend section, and the bend section is provided with several air outlet holes.

[0016] Preferably, one end of the guide tube is provided with an airflow regulating component;

[0017] The air volume regulating component includes a sliding groove opened at one end of the support plate, a sliding block slidably disposed in the sliding groove, a baffle plate disposed on the sliding block, and multiple baffle plates forming a baffle ring.

[0018] Preferably, a rotating rod that is threadedly connected to the sliding block is rotatably provided in the sliding groove, and a transmission gear is provided at the upper end of the rotating rod after it rotatably passes through the sleeve. Multiple gear rings that mesh with the transmission gear are rotatably provided on the sleeve.

[0019] Preferably, one end of the sleeve is provided with an air inlet regulating component;

[0020] The air inlet regulating component includes a connecting cylinder at one end of the sleeve, a connecting ring inside the connecting cylinder, several air inlet holes on the sleeve and the guide cylinder, a partition on one side of the connecting ring, a connecting rod slidingly passing through the partition, and a windproof cone corresponding to the connecting ring at one end of the connecting rod.

[0021] A return spring is provided between the partition and the piston, and an air guide pipe is provided on the connecting cylinder between the partition and the connecting ring. The air guide pipe is connected to the outer cylinder.

[0022] Preferably, a piston is slidably provided at one end of the connecting rod, and the piston and one end of the connecting cylinder form a receiving cavity. A receiving bottle is located inside the outer cylinder, and a conduit is provided between the receiving bottle and the receiving cavity.

[0023] Preferably, a light fixture is provided between the outer cylinder and the semi-cylinder;

[0024] The lighting device includes a gap formed between a semi-cylinder and an outer cylinder, a rotating ring is rotatably installed in the gap, a lamp plate is installed on the rotating ring, and several lamp beads are installed at the lower end of the lamp plate.

[0025] Preferably, a toothed ring is provided on the rotating ring, and a drive gear that meshes with the toothed ring is rotatably provided at the bottom of the outer cylinder.

[0026] In summary, this application includes at least one of the following beneficial technical effects:

[0027] 1. This invention uses a pump to circulate the refrigerant within the refrigerant pipe. During operation, a fan at one end of the sleeve is activated, causing airflow to circulate within the annular cavity and guide tube. The airflow blows onto the spiral section of the refrigerant pipe, releasing its cooling capacity as the refrigerant flows through the tortuous section. This ensures that the differences in cold resistance of different coffee varieties under uniform low-temperature stress conditions can be accurately captured, resulting in high repeatability and reliability of the test data.

[0028] 2. In this invention, the airflow blown out from the guide tube is captured by an air collector at one end. The airflow flows within the space between the air inlet pipe and the cooling pipe, making full contact with the low-temperature cooling pipe for further cooling. Combined with the precise coverage of the cultivation tray by the air outlet, directional delivery of cold energy is achieved. Simultaneously, relying on the airflow regulator to precisely allocate the airflow ratio and the air inlet regulator to automatically switch between internal and external circulation, the temperature gradient within the cultivation chamber can be stably controlled, avoiding interference from environmental fluctuations.

[0029] 3. This invention achieves uniform light sweep across all cultivation chambers by driving gear transmission, which avoids the problem of local brightness difference in fixed lighting and simulates the dynamic characteristics of natural lighting. In addition, the lamp plate is located at the lower end of the curved section of the cooling pipe, which can indirectly cool the lamp by utilizing the cooling capacity, thus extending the life of the lamp. At the same time, the full-spectrum lighting parameters are adapted to the light requirements of coffee seed germination, providing a more realistic environmental basis for cold resistance testing. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the structure of the present invention.

[0031] Figure 2 This is a cross-sectional view of the outer cylinder of the present invention.

[0032] Figure 3 This is an exploded view of the outer cylinder and the culture tray of the present invention.

[0033] Figure 4 This is a schematic diagram of the structure of the cooler of the present invention.

[0034] Figure 5 This is the present invention. Figure 4 A magnified view of part A.

[0035] Figure 6 This is a cross-sectional view of the cooler of the present invention.

[0036] Figure 7 This is a schematic diagram of the structure between the air collecting hood and the air inlet pipe of the present invention.

[0037] Figure 8 This is a schematic diagram of the air volume regulating component of the present invention.

[0038] Figure 9 This is the present invention. Figure 8 A magnified view of section B.

[0039] Figure 10 This is a schematic diagram of the air intake regulating component of the present invention.

[0040] Figure 11 This is a planar sectional view of the air inlet regulating component of the present invention.

[0041] Figure 12 This is a schematic diagram of the structure between the cooler and the lighting device of the present invention.

[0042] Figure 13 This is a schematic diagram of the structure between the outer cylinder, the cultivation tray and the illuminator of the present invention.

[0043] Figure 14 This is a schematic diagram of the structure of the lighting device of the present invention.

[0044] Explanation of reference numerals in the attached figures:

[0045] 1. Outer cylinder; 11. Protrusion; 2. Semi-cylinder; 3. Culture tray; 31. Culture chamber; 4. Glass window; 5. Refrigerator; 51. Sleeve; 52. Fan; 53. Support plate; 54. Guide tube; 55. Annular cavity; 56. Refrigeration pipe; 561. Spiral section; 562. Bending section; 57. Air collector hood; 571. Gap; 58. Air inlet pipe; 581. Bending section; 582. Air outlet; 6. Air volume regulating component; 61. Sliding groove; 62. Sliding block; 63. Wind deflector; 64. Wind deflector ring; 65. Rotating rod; 66. Transmission gear; 67. Gear ring; 7. Air inlet adjustment component; 71. Air inlet hole; 72. Connecting cylinder; 73. Connecting ring; 74. Partition plate; 75. Connecting rod; 751. Wind deflector cone; 752. Piston; 76. Air guide pipe; 77. Receiving cavity; 78. Conduit; 79. Return spring; 8. Lighting device; 81. Gap; 82. Rotating ring; 83. Light panel; 84. Gear ring; 85. Drive gear. Detailed Implementation

[0046] The following is in conjunction with the appendix Figures 1 to 14 This application will be described in further detail.

[0047] This application discloses a cold resistance simulation testing device for coffee germplasm, which can ensure that the grinding powder and polishing liquid are fully mixed and stirred, and avoid the grinding powder from clumping in the polishing liquid, thus affecting the mixing effect.

[0048] Example 1:

[0049] When testing the cold resistance of coffee germplasm, coffee germplasm is planted in a specific area, the area is cooled and the humidity is controlled to simulate a low-temperature environment, and the germination of coffee germplasm is tested under low-temperature conditions to achieve the purpose of testing the cold resistance of coffee germplasm.

[0050] Reference Figure 1 ,Figure 2 and Figure 3 The present invention relates to a coffee germplasm cold resistance simulation testing device, comprising an outer cylinder 1, a semi-cylinder 2 disposed inside the outer cylinder 1, a detachable cultivation tray 3 slidably disposed inside the semi-cylinder 2, glass windows 4 disposed on both sides of the cultivation tray 3, and a plurality of evenly distributed cultivation chambers 31 constructed inside the cultivation tray 3.

[0051] When testing the cold resistance of coffee germplasm, the coffee germplasm is first pretreated. Plump seeds of the germplasm to be tested are selected, disinfected with sodium hypochlorite solution, rinsed with deionized water, and then soaked in distilled water until they swell. Then, the cultivation tray 3 is pulled out. One side of the glass window 4 is connected to the semi-cylinder 2 and remains stationary, while the other side is connected to the cultivation tray 3 and pulled out with the cultivation tray 3. Then, sterilized seedling substrate is filled into each cultivation chamber 31.

[0052] One pre-treated coffee seed is sown into each cultivation chamber 31. After sowing, the cultivation tray 3 is pushed back to its original position along the slide rail of the semi-cylinder 2, so that the glass windows 4 on both sides are sealed and fitted together, ensuring that a closed environment is formed inside the semi-cylinder 2.

[0053] The refrigerator 5 on one side of the outer cylinder 1 is activated, and cold air enters from the protrusion 11 located directly above the cultivation tray 3 on the outer cylinder 1. The germination status of seeds in each cultivation chamber 31 is observed and recorded through the glass window 4, and the germination rate is calculated. If it is necessary to determine the extreme cold resistance temperature, the temperature is lowered until no new germination occurs and the seeds die. Finally, the cold resistance of the germplasm is evaluated based on the germination data.

[0054] Reference Figure 4 , Figure 5 and Figure 6 As shown, specifically, the cooler 5 includes a sleeve 51 provided on one side of the outer cylinder 1. Multiple circumferentially evenly distributed support plates 53 are provided inside the sleeve 51. One end of the multiple support plates 53 is provided with a guide tube 54. The guide tube 54 and the sleeve 51 rotate to form an annular cavity 55. A cooling pipe 56 is provided in the annular cavity 55 through the support plates 53. Both ends of the cooling pipe 56 penetrate into the protrusion 11, and the two ends of the cooling pipe 56 are interconnected to form a circuit.

[0055] The refrigerant is injected into the refrigerant pipe 56 and is circulated in the refrigerant pipe 56 by pumping. When working, the fan 52 set at one end of the sleeve 51 is started. The fan 52 blows airflow in the annular cavity 55 and the guide tube 54. The airflow blows on the spiral section 561 set in the annular cavity 55 on the refrigerant pipe 56. The cooled refrigerant releases its cooling capacity when it flows through the tortuous section 562 located in the protrusion 11 on the refrigerant pipe 56.

[0056] Reference Figure 6 and Figure 7As shown, during this process, the airflow blown out of the guide tube 54 is captured by the air collecting hood 57 set at one end. The air collecting hood 57 is connected to the air inlet pipe 58. One end of the air inlet pipe 58 passes through the protrusion 11 and is sleeved on the outside of the cooling pipe 56. The airflow will enter the bend section 581 set on the air inlet pipe 58 corresponding to the bend section 562. The airflow will flow in the interlayer space between the air inlet pipe 58 and the cooling pipe 56, and make full contact with the low temperature cooling pipe 56 for further cooling. Then it will be blown out through several air outlets 582 opened on the bend section 581. Several air outlets 582 are distributed directly above the cultivation tray 3 to accurately deliver cold energy to each cultivation chamber 31.

[0057] The fan 52 drives the airflow from the guide tube 54 into the annular cavity 55, and flows through the spiral section 561 of the cooling pipe 56 for preliminary heat exchange. Most of the airflow is captured by the air collector shroud 57 and guided into the air inlet pipe 58, while a small portion of the airflow is discharged through the gap 571 reserved between the air collector shroud 57 and the guide tube 54, maintaining the airflow pressure balance in the annular cavity 55 and ensuring smoother airflow circulation driven by the fan 52.

[0058] Humidity can be controlled by humidifying the air inlet duct 58 with a humidifier (existing technology) and allowing the moisture to enter the outer cylinder 1 with the airflow.

[0059] Reference Figure 8 and Figure 9 As shown, an airflow regulating component 6 is provided at one end of the guide tube 54. The airflow regulating component 6 can distribute the airflow into the annular cavity 55 and the guide tube 54. By adjusting the airflow ratio between the two, the heat exchange efficiency between the airflow and the spiral section 561 of the cooling pipe 56 can be flexibly controlled, thereby adjusting the intensity of the cold air finally delivered to the cultivation tray 3 to adapt to the different requirements of different coffee varieties for low-temperature environment in the test and improve the accuracy of low-temperature simulation.

[0060] Specifically, the airflow regulating component 6 includes a sliding groove 61 opened at one end of the support plate 53, a sliding block 62 slidably disposed in the sliding groove 61, a baffle plate 63 disposed on the side of each sliding block 62 facing the center of the guide tube 54, and multiple baffle plates 63 spliced ​​together in the circumferential direction to form an openable and closable annular baffle ring 64. A rotating rod 65 is rotatably disposed in the sliding groove 61 along the length direction, the rotating rod 65 is threadedly connected to the sliding block 62, and a transmission gear 66 is disposed at the upper end of the rotating rod 65 after rotating through the sleeve 51. Multiple gear rings 67 that mesh with the transmission gear 66 are rotatably disposed on the sleeve 51.

[0061] During adjustment, rotating the gear ring 67 can synchronously drive all transmission gears 66 to rotate, causing the rotating rod 65 to rotate, so that the sliding block 62 moves synchronously closer to or further away from the center of the guide cylinder 54 along the sliding groove 61; when the sliding block 62 drives the baffle plate 63 to converge towards the center, the inner diameter of the baffle ring 64 decreases, blocking the ventilation gap 571 between the guide cylinder 54 and the annular cavity 55, reducing the amount of air entering the annular cavity 55 and increasing the amount of air flowing through the inside of the guide cylinder 54.

[0062] Conversely, when the sliding block 62 moves the wind deflector 63 outward, the inner diameter of the wind deflector ring 64 increases, the ventilation gap 571 expands, the air volume entering the annular cavity 55 increases, and the air volume in the guide tube 54 decreases, thereby accurately distributing the air volume ratio between the two.

[0063] Reference Figure 10 and Figure 11 As shown, one end of the sleeve 51 is equipped with an air inlet regulator 7. The air inlet regulator 7 can flexibly select the airflow circulation mode according to the test requirements. During the low temperature rapid cooling stage, external circulation is used to accelerate heat dissipation by utilizing external airflow. During the constant temperature stabilization stage, internal circulation is switched to reduce the interference of environmental fluctuations on the temperature and humidity of the incubation chamber 31 and improve the stability of low temperature simulation.

[0064] Specifically, the air inlet regulating component 7 includes a connecting cylinder 72 provided at one end of the sleeve 51. The sleeve 51 and the guide cylinder 54 are provided with a plurality of air inlet holes 71. A connecting ring 73 is provided inside the connecting cylinder 72. A partition 74 is provided on one side of the connecting ring 73. A connecting rod 75 is slidably passed through the partition 74. A wind-blocking cone 751 corresponding to the connecting ring 73 is provided at one end of the connecting rod 75. An air guide pipe 76 is provided on the connecting cylinder 72 between the partition 74 and the connecting ring 73. The guide pipe 76 is connected to the outer cylinder 1. A piston 752 is slidably provided at one end of the connecting rod 75. The piston 752 and one end of the connecting cylinder 72 form a receiving cavity 77. A receiving bottle (not shown in the figure) is provided inside the outer cylinder 1. A guide pipe 78 is provided between the receiving bottle and the receiving cavity 77.

[0065] Hydraulic oil is injected into both the receiving bottle and the receiving cavity 77. In the initial state, the outer cylinder 1 is at room temperature, and the hydraulic oil in the receiving bottle and the receiving cavity 77 is in a state of pressure balance. The hydraulic oil in the receiving cavity 77 pushes the piston 752 to move towards the partition 74, compressing the return spring 79 between the partition 74 and the piston 752, causing the connecting rod 75 to move the wind deflector 751 away from the connecting ring 73, forming a gap 571 between the wind deflector 751 and the connecting ring 73. When the fan 52 is started, the airflow in the outer cylinder 1 enters the space between the connecting ring 73 and the partition 74 through the air guide pipe 76, and then passes through the gap 571 between the wind deflector 751 and the connecting ring 73 to enter the sleeve 51. After mixing with the airflow entering from the outside through the air inlet 71, it participates in heat exchange.

[0066] When the temperature inside the outer cylinder 1 decreases, the hydraulic oil in the receiving bottle shrinks due to the cold. Under the elastic force of the return spring 79, the hydraulic oil in the receiving cavity 77 flows back to the receiving bottle through the conduit 78. The piston 752 moves away from the partition 74 as the hydraulic oil decreases, causing the connecting rod 75 and the wind deflector 751 to move closer to the connecting ring 73, reducing the gap 571 between the wind deflector 751 and the connecting ring 73, reducing the amount of airflow entering the sleeve 51 from the outer cylinder 1. At the same time, the amount of outside air entering through the air inlet 71 increases relatively, thereby automatically adjusting the ratio of internal and external airflow circulation to meet the heat exchange requirements in low-temperature environments.

[0067] Due to the obstruction of the connecting cylinder 72, after the fan 52 is started, the air in the annular cavity 55 and the guide cylinder 54 is continuously blown out, forming a negative pressure effect. At this time, the outside air will automatically fill into the annular cavity 55 and the guide cylinder 54 through the air inlet 71 on the sleeve 51 and the guide cylinder 54 to replenish the amount of air blown out.

[0068] At initial ambient temperature, the wind deflector 751 and the connecting ring 73 maintain a gap 571. The airflow inside the outer cylinder 1 enters the sleeve 51 through the air guide pipe 76 and the gap 571, mixing with the outside air introduced by the air inlet 71, and jointly participating in the heat exchange cycle within the annular cavity 55. When the temperature inside the outer cylinder 1 decreases, the hydraulic oil in the receiving bottle contracts, and the return spring 79 pushes the piston 752 to move the wind deflector 751 closer to the connecting ring 73, reducing the gap 571. This reduces the amount of airflow entering the outer cylinder 1, and the proportion of outside air introduced by the air inlet 71 increases accordingly. Through this automatically adjusted airflow replenishment method, a stable airflow cycle is maintained within the annular cavity 55 and the guide pipe 54, providing a continuous heat exchange medium for the spiral section 561 of the refrigeration pipe 56, ensuring precise and controllable low-temperature environment in the cultivation tray 3 area.

[0069] Example 2:

[0070] Reference Figure 12 , Figure 13 and Figure 1 As shown, based on Example 1, an illuminator 8 is provided between the outer cylinder 1 and the semi-cylinder 2. The illuminator 8 continuously provides stable light. After the light penetrates the semi-cylinder 2, it covers all the cultivation chambers 31 of the cultivation tray 3, ensuring that the seeds in each chamber receive uniform light.

[0071] Specifically, the lighting device 8 includes a gap 81 formed between the semi-cylinder 2 and the outer cylinder 1. A rotating ring 82 is rotatably disposed in the gap 81. A lamp plate 83 located at the lower end of the bend section 562 is disposed on the rotating ring 82. Several lamp beads (not shown in the figure) are disposed at the lower end of the lamp plate 83. A toothed ring 84 is disposed on the rotating ring 82. A drive gear 85 that meshes with the toothed ring 84 is rotatably disposed at the bottom of the outer cylinder 1.

[0072] During operation, the drive motor drives the drive gear 85 to rotate, and the gear ring 84 drives the rotating ring 82 to rotate slowly in the gap 81. The lamp plate 83 rotates synchronously with the rotating ring 82, so that the light emitted by the lamp beads can evenly sweep across each cultivation chamber 31 of the cultivation tray 3, avoiding local brightness differences caused by fixed lighting.

[0073] Meanwhile, the lamp board 83 is located at the lower end of the curved section 562, which can indirectly reduce the working temperature of the lamp beads by utilizing the cooling energy emitted by the cooling pipe 56, thus extending its service life. Moreover, the rotating lighting mode is closer to the dynamic characteristics of natural light, enhancing the simulation of the coffee seed germination environment.

[0074] The implementation principle of this invention is as follows:

[0075] (1): Select plump seeds of coffee varieties to be tested, disinfect them with sodium hypochlorite solution, rinse them with deionized water, soak them in distilled water until they swell, dry the surface moisture with sterile filter paper, pull out the cultivation tray 3, fill each cultivation chamber 31 with sterile seedling substrate, sow one pretreated seed in each chamber, push the cultivation tray 3 back to seal the glass windows 4 on both sides, forming a closed test chamber.

[0076] (2): The refrigerant is injected into the refrigerant pipe 56. The refrigerant is circulated in the refrigerant pipe 56 by pumping. When working, the fan 52 set at one end of the sleeve 51 is started. The fan 52 blows airflow in the annular cavity 55 and the guide tube 54. The airflow blows on the spiral section 561 set in the annular cavity 55 on the refrigerant pipe 56. The cooled refrigerant releases cold energy when it flows through the tortuous section 562 in the protrusion 11 on the refrigerant pipe 56.

[0077] (3): The airflow blown out of the guide tube 54 will be captured by the air collecting hood 57 set at one end. The air collecting hood 57 is connected to the air inlet pipe 58. One end of the air inlet pipe 58 passes through the protrusion 11 and is sleeved on the outside of the cooling pipe 56. The airflow will enter the bend section 581 set on the air inlet pipe 58 corresponding to the bend section 562. The airflow will flow in the interlayer space between the air inlet pipe 58 and the cooling pipe 56, and make full contact with the low temperature cooling pipe 56 to further cool down. Then it will be blown out by several air outlets 582 set on the bend section 581. Several air outlets 582 are distributed directly above the cultivation tray 3 to accurately deliver cold energy to each cultivation tray 3.

[0078] (4): During adjustment, rotating the gear ring 67 can synchronously drive all transmission gears 66 to rotate, causing the rotating rod 65 to rotate, so that the sliding block 62 moves closer to or further away from the center of the guide cylinder 54 along the sliding groove 61. When the sliding block 62 drives the baffle plate 63 to converge towards the center, the inner diameter of the baffle ring 64 decreases, blocking the ventilation gap 571 between the guide cylinder 54 and the annular cavity 55, reducing the amount of air entering the annular cavity 55 and increasing the amount of air flowing through the inside of the guide cylinder 54.

[0079] Conversely, when the sliding block 62 moves the wind deflector 63 outward, the inner diameter of the wind deflector ring 64 increases, the ventilation gap 571 expands, the air volume entering the annular cavity 55 increases, and the air volume in the guide tube 54 decreases, thereby accurately distributing the air volume ratio between the two.

[0080] (5): Hydraulic oil is injected into both the receiving bottle and the receiving cavity 77. In the initial state, the outer cylinder 1 is at room temperature, and the hydraulic oil in the receiving bottle and the receiving cavity 77 is in a pressure balance state. The hydraulic oil in the receiving cavity 77 pushes the piston 752 to move towards the partition 74, compressing the return spring 79 between the partition 74 and the piston 752, so that the connecting rod 75 drives the wind deflector 751 away from the connecting ring 73, forming a gap 571 between the wind deflector 751 and the connecting ring 73. When the fan 52 starts, the airflow in the outer cylinder 1 enters the space between the connecting ring 73 and the partition 74 through the air guide pipe 76, and then enters the sleeve 51 through the gap 571 between the wind deflector 751 and the connecting ring 73. It then mixes with the airflow entering from the outside through the air inlet 71 and participates in heat exchange.

[0081] (6): When the temperature inside the outer cylinder 1 decreases, the hydraulic oil in the receiving bottle shrinks due to the cold contraction. Under the elastic force of the return spring 79, the hydraulic oil in the receiving cavity 77 flows back to the receiving bottle through the conduit 78. The piston 752 moves away from the partition 74 as the hydraulic oil decreases, driving the connecting rod 75 and the wind deflector 751 to move closer to the connecting ring 73, reducing the gap 571 between the wind deflector 751 and the connecting ring 73, reducing the amount of airflow entering the sleeve 51 inside the outer cylinder 1. At the same time, the amount of outside air entering through the air inlet 71 increases relatively, thereby automatically adjusting the ratio of internal and external airflow circulation to meet the heat exchange requirements in low temperature environments.

[0082] The embodiments described herein are preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made in accordance with the structure, shape, and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A cold resistance simulation testing device for coffee germplasm, comprising an outer cylinder (1) and a semi-cylinder (2) disposed inside the outer cylinder (1), characterized in that: A detachable culture tray (3) is slidably arranged inside the semi-cylinder (2), and a protrusion (11) located directly above the culture tray (3) is provided on the outer cylinder (1). A cooler (5) is provided on one side of the outer cylinder (1). The cooler (5) includes a sleeve (51) provided on one side of the outer cylinder (1). Multiple circumferentially evenly distributed support plates (53) are provided inside the sleeve (51). One end of the multiple support plates (53) is provided with a guide tube (54). The guide tube (54) and the sleeve (51) rotate to form an annular cavity (55). A cooling pipe (56) is provided inside the annular cavity (55) through the support plates (53). Both ends of the cooling pipe (56) penetrate into the protrusion (11), and the two ends of the cooling pipe (56) are interconnected to form a circuit.

2. The coffee germplasm cold resistance simulation testing device according to claim 1, characterized in that: The cooling pipe (56) is provided with a spiral section (561) located in the annular cavity (55) and a tortuous section (562) located in the protrusion (11).

3. The coffee germplasm cold resistance simulation testing device according to claim 2, characterized in that: One end of the guide tube (54) is provided with an air collecting hood (57), and an air inlet pipe (58) is connected to the air collecting hood (57); One end of the air inlet pipe (58) passes through the protrusion (11) and is then fitted over the refrigeration pipe (56).

4. The coffee germplasm cold resistance simulation testing device according to claim 3, characterized in that: Furthermore, the air inlet pipe (58) is provided with a bend section (581) corresponding to the bend section (562), and the bend section (581) is provided with several air outlet holes (582).

5. The coffee germplasm cold resistance simulation testing device according to claim 1, characterized in that: One end of the guide tube (54) is provided with an air volume regulating component (6); The air volume regulating component (6) includes a sliding groove (61) opened at one end of the support plate (53), a sliding block (62) is slidably arranged in the sliding groove (61), a baffle plate (63) is arranged on the sliding block (62), and multiple baffle plates (63) form a baffle ring (64).

6. The coffee germplasm cold resistance simulation testing device according to claim 5, characterized in that: A rotating rod (65) is rotatably installed in the sliding groove (61) and threadedly connected to the sliding block (62). The upper end of the rotating rod (65) is rotatably installed through the sleeve (51) and a transmission gear (66) is installed thereon. Multiple gear rings (67) that mesh with the transmission gear (66) are rotatably installed on the sleeve (51).

7. The coffee germplasm cold resistance simulation testing device according to claim 1, characterized in that: An air inlet regulating component (7) is provided at one end of the sleeve (51); The air inlet regulating component (7) includes a connecting cylinder (72) provided at one end of a sleeve (51), a connecting ring (73) provided inside the connecting cylinder (72), a plurality of air inlet holes (71) provided on the sleeve (51) and the guide cylinder (54), a partition (74) provided on one side of the connecting ring (73), a connecting rod (75) slidably passing through the partition (74), and a wind deflector (751) corresponding to the connecting ring (73) provided at one end of the connecting rod (75); A return spring (79) is provided between the partition (74) and the piston (752), and an air duct (76) is provided on the connecting cylinder (72) between the partition (74) and the connecting ring (73). The duct (78) is connected to the outer cylinder (1).

8. The coffee germplasm cold resistance simulation testing device according to claim 7, characterized in that: A piston (752) is slidably provided at one end of the connecting rod (75). The piston (752) and one end of the connecting cylinder (72) form a receiving cavity (77). There is a receiving bottle inside the outer cylinder (1). A conduit (78) is provided between the receiving bottle and the receiving cavity (77).

9. The coffee germplasm cold resistance simulation testing device according to claim 1, characterized in that: A lighting device (8) is provided between the outer cylinder (1) and the semi-cylinder (2); The lighting device (8) includes a gap (81) formed between the semi-cylinder (2) and the outer cylinder (1), a rotating ring (82) is rotatably arranged in the gap (81), a lamp plate (83) is arranged on the rotating ring (82), and a number of lamp beads are arranged at the lower end of the lamp plate (83).

10. The coffee germplasm cold resistance simulation testing device according to claim 9, characterized in that: A toothed ring (84) is provided on the rotating ring (82), and a drive gear (85) that meshes with the toothed ring (84) is rotatably provided at the bottom of the outer cylinder (1).

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