Coffee composition with memory improving function and application thereof

Abstract

The invention relates to the technical field of food biology, in particular to a coffee composition with a memory improving function and application of the coffee composition. The coffee composition comprises the following components in parts by weight: 3 parts of Clerodendranthus spicatus polypeptide, 1 part of nervonic acid and 16 parts of Korean coffee. The composition can activate an Nrf2 / HO-1 signal channel to relieve the oxidative stress reaction of the hippocampus and improve the non-space learning ability and memory ability. The traditional Chinese medicine composition is high in safety, few in side effect and mild in effect, is suitable for early prevention and long-term conditioning of people with hypomnesis, and is particularly suitable for people with natural memory decline caused by age increase and people with hypomnesis caused by overstrain of brain.

Description

Technical Field

[0001] This invention relates to the field of food biotechnology, and in particular to a coffee composition with memory-improving function and its application. Background Technology

[0002] Memory decline refers to a decrease in the brain's ability to store and recall information, often manifesting as difficulty remembering recent events, learning new knowledge, or retrieving past memories. Its inducing factors are complex and diverse, mainly falling into two categories: first, physiological factors, such as the natural aging process, genetic factors, and neurodegenerative diseases like Alzheimer's disease; second, exogenous factors, including long-term mental stress and sleep deprivation, nutritional imbalances due to poor dietary habits, brain trauma, cardiovascular diseases affecting cerebral blood supply, as well as certain drug side effects, thyroid dysfunction, and psychosomatic health problems such as depression. These factors may act alone or in combination, damaging brain cell function and connections, thereby leading to memory decline.

[0003] Currently, treatment for memory loss primarily involves medication, cognitive training, lifestyle modifications, and management of cardiovascular risk factors. However, existing methods have significant limitations. First, while mainstream medications can alleviate symptoms to some extent, they cannot reverse or halt the fundamental progression of the disease and may cause side effects such as nausea and dizziness. Second, the effectiveness of non-pharmacological methods varies from person to person, making standardization and sustainability difficult. The lack of precise, personalized treatment plans tailored to different causes, such as genetic, metabolic, and vascular factors, results in limited overall efficacy and fails to meet the enormous clinical demand. Therefore, there is an urgent need for effective methods that are highly safe, have few side effects, and are gentle in their effects. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a coffee composition for improving memory function and its application. The composition can enhance non-spatial learning and memory abilities by activating the Nrf2 / HO-1 signaling pathway, reducing oxidative stress in the hippocampus. This invention is highly safe, has few side effects, and is gentle in its effects, making it suitable for early prevention and long-term management of memory decline, especially for those experiencing natural memory decline due to age and those whose memory loss is caused by excessive mental exertion.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] The present invention provides a coffee composition with memory-improving function, comprising the following components in parts by weight: 3 parts of ginseng polypeptide, 1 part of nervonic acid and 16 parts of decaffeinated coffee.

[0007] Preferably, the preparation method of the ginseng polypeptide includes the following steps:

[0008] 1) Mix the ginseng powder with an ethanol solution and extract by ultrasonication. After centrifugation, filter the supernatant under reduced pressure to obtain the filtrate.

[0009] 2) Pass the filtrate obtained in step 1) through an AB-8 macroporous resin column and elute with water, 5% ethanol solution, 15% ethanol solution and 25% ethanol solution, respectively, for 2 hours, 2 hours, 3 hours and 4 hours. After mixing the solutions eluted with ethanol solution, dry at 40°C to constant weight to obtain ginseng polypeptide.

[0010] Preferably, the ratio of the ginseng powder to the ethanol solution in step 1) is 1:10 g / mL;

[0011] The volume percentage of the ethanol solution is 65%;

[0012] The conditions for ultrasonic extraction include: ultrasonic power of 350W and extraction time of 35 minutes;

[0013] The centrifugation conditions include: centrifugation at 3000 rpm for 10 minutes.

[0014] The conditions for the reduced pressure filtration include: medium-speed qualitative filter paper with a pore size of 10 μm and a filtration pressure of 0.08 MPa.

[0015] Preferably, the method for preparing the nervonic acid includes the following steps:

[0016] 1) Crush and dry the seeds of Acer truncatum to obtain Acer truncatum seed powder;

[0017] 2) The Acer truncatum seed powder described in step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 32 MPa, the separation temperature is 58°C, the extraction temperature is 45°C, and the extraction time is 1.5 hours. The material in the separation vessel is then collected.

[0018] 3) The material collected in step 2) is subjected to low-temperature crystallization. After crystallization is completed, solid-liquid separation is performed, and the liquid phase component is collected.

[0019] 4) Perform molecular distillation on the liquid phase component obtained in step 3), collect the distillate, and obtain nervonic acid. Preferably, the conditions for low-temperature crystallization in step 3) are: temperature -20°C and time 10 hours;

[0020] The solid-liquid separation method is vacuum filtration, and the conditions for vacuum filtration include: medium-speed qualitative filter paper with a pore size of 10 μm and a filtration pressure of 0.08 MPa.

[0021] Preferably, the conditions for molecular distillation in step 4) are: pressure of 1.5 Pa and evaporation surface temperature of 165 °C.

[0022] Preferably, the method for preparing the decaffeinated coffee includes the following steps:

[0023] 1) Grind and dry the coffee beans to obtain coffee powder;

[0024] 2) The coffee powder described in step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 20 MPa, the separation temperature is 58°C, the extraction temperature is 50°C, and the extraction time is 1.5 hours.

[0025] 3) Collect the solid material in the extraction vessel from step 2) to obtain decaffeinated coffee.

[0026] The present invention also provides a method for preparing the coffee composition described above, comprising the following steps:

[0027] The ginseng polypeptide, nervonic acid, and decaffeinated coffee are uniformly mixed to form a coffee composition.

[0028] The present invention also provides the application of the coffee composition described above in the preparation of products for alleviating memory decline and assisting in improving memory.

[0029] Preferably, the product is food, medicine, or health product.

[0030] Ginseng polypeptides, serving as a nutrient base for nerve cell growth, can promote neuronal survival and synaptic plasticity, providing a structural basis for memory function. Nervonic acid is a key component for the repair of brain nerve fibers, helping to enhance the efficiency of nerve signal transmission and improve cognitive pathways. Low-caffeine coffee, while avoiding the side effects of high caffeine such as anxiety and insomnia, reduces nerve inflammation and oxidative stress damage through its antioxidant components, such as chlorogenic acid. These three components work synergistically through multiple pathways of neurotrophic, neurorepair, and neuroprotective mechanisms, thereby achieving a comprehensive relief of memory decline.

[0031] The beneficial effects of this invention are:

[0032] The composition provided by this invention can reduce oxidative stress in the hippocampus and improve non-spatial learning and memory abilities by activating the Nrf2 / HO-1 signaling pathway. This invention is highly safe, has few side effects, and is gentle in its effects, making it suitable for early prevention and long-term management of memory decline, especially for those experiencing natural memory decline due to age and those whose memory decline is caused by excessive mental exertion. Attached Figure Description

[0033] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0034] Figure 1The effect of the composition on the ability of mice to recognize new objects; (A) the total exploration time of mice for new and old objects; (B) the discrimination index of mice for new objects;

[0035] Figure 2 The effect of the composition on the activity of SOD and GSH-Px and the level of MDA in mouse serum (A) and hippocampus (B);

[0036] Figure 3 The composition was designed to protect against D-galactose-induced oxidation by upregulating the Nrf2 / HO-1 signaling pathway in the brain. (A) Western blot analysis was performed on the protein expression levels of Nrf2 and Nucl-Nrf2, and grayscale quantitative analysis was performed. (B) Western blot analysis was performed on the protein expression levels of HO-1 and NQO1, and grayscale quantitative analysis was performed. Detailed Implementation

[0037] The present invention provides a coffee composition with memory-improving function, comprising the following components in parts by weight: 3 parts of ginseng polypeptide, 1 part of nervonic acid and 16 parts of decaffeinated coffee.

[0038] In this invention, the preparation method of the ginseng polypeptide preferably includes the following steps:

[0039] 1) Mix the ginseng powder with an ethanol solution and extract by ultrasonication. After centrifugation, filter the supernatant under reduced pressure to obtain the filtrate.

[0040] 2) Pass the filtrate obtained in step 1) through an AB-8 macroporous resin column and elute with water, 5% ethanol solution, 15% ethanol solution and 25% ethanol solution, respectively, for 2 hours, 2 hours, 3 hours and 4 hours. After mixing the solutions eluted with ethanol solution, dry at 40°C to constant weight to obtain ginseng polypeptide.

[0041] In this invention, the preferred material-to-liquid ratio of the ginseng powder to the ethanol solution is 1:10 g / mL. The preferred volume percentage of the ethanol solution is 65%. The preferred conditions for ultrasonic extraction are: ultrasonic power of 350 W and extraction time of 35 minutes. The preferred conditions for centrifugation are: centrifugation at 3000 rpm for 10 minutes. The preferred conditions for vacuum filtration are: medium-speed qualitative filter paper with a pore size of 10 μm and a filtration pressure of 0.08 MPa.

[0042] In this invention, the method for preparing nervonic acid preferably includes the following steps:

[0043] 1) Crush and dry the seeds of Acer truncatum to obtain Acer truncatum seed powder;

[0044] 2) The Acer truncatum seed powder described in step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 32 MPa, the separation temperature is 58°C, the extraction temperature is 45°C, and the extraction time is 1.5 hours. The material in the separation vessel is then collected.

[0045] 3) The material collected in step 2) is subjected to low-temperature crystallization. After crystallization is completed, solid-liquid separation is performed, and the liquid phase component is collected.

[0046] 4) Perform molecular distillation on the liquid phase component obtained in step 3), collect the distillate, and obtain nervonic acid.

[0047] In this invention, the preferred conditions for low-temperature crystallization are: a temperature of -20°C and a time of 10 hours. In this invention, the preferred method for solid-liquid separation is vacuum filtration, and the preferred conditions for vacuum filtration are: a medium-speed qualitative filter paper with a pore size of 10 μm and a filtration pressure of 0.08 MPa. In this invention, the preferred conditions for molecular distillation are: a pressure of 1.5 Pa and an evaporation surface temperature of 165°C.

[0048] 7. In this invention, the method for preparing the decaffeinated coffee preferably includes the following steps:

[0049] 1) Grind and dry the coffee beans to obtain coffee powder;

[0050] 2) The coffee powder described in step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 20 MPa, the separation temperature is 58°C, the extraction temperature is 50°C, and the extraction time is 1.5 hours.

[0051] 3) Collect the solid material in the extraction vessel from step 2) to obtain decaffeinated coffee.

[0052] The present invention also provides a method for preparing the coffee composition described in the above technical solution, comprising the following steps: uniformly mixing the osmosin polypeptide, nervonic acid and decaffeinated coffee to form the coffee composition.

[0053] This invention also provides the application of the coffee composition described above in the preparation of products for alleviating memory decline and assisting in improving memory. In this invention, the product is preferably a food, pharmaceutical, or health product.

[0054] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0055] Example 1

[0056] A coffee composition for improving memory function comprises the following components in parts by weight: 3 parts of ginseng polypeptide, 1 part of nervonic acid, and 16 parts of decaffeinated coffee.

[0057] The preparation method of the ginseng polypeptide includes the following steps:

[0058] 1) After mixing the ginseng powder with an ethanol solution, ultrasonic extraction was performed. After centrifugation, the supernatant was filtered under reduced pressure to obtain the filtrate. The ratio of ginseng powder to ethanol solution was 1:10 g / mL, and the volume percentage of ethanol solution was 65%. The ultrasonic extraction conditions included: ultrasonic power of 350W, extraction time of 35 minutes, centrifugation conditions included: centrifugation at 3000 rpm for 10 minutes, and reduced pressure filtration conditions included: medium-speed qualitative filter paper with a pore size of 10 μm and filtration pressure of 0.08 MPa.

[0059] 2) Pass the filtrate from step 1) through an AB-8 macroporous resin column and elute with water, 5% ethanol solution, 15% ethanol solution and 25% ethanol solution, respectively, for 2 hours, 2 hours, 3 hours and 4 hours. After mixing the solutions eluted with ethanol, dry at 40°C to constant weight to obtain ginseng polypeptide.

[0060] The preparation method of nervonic acid is as follows:

[0061] 1) Crush and dry the seeds of Acer truncatum to obtain Acer truncatum seed powder;

[0062] 2) The Acer truncatum seed powder from step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 32 MPa, the separation temperature is 58°C, the extraction temperature is 45°C, and the extraction time is 1.5 hours. The material in the separation vessel is then collected.

[0063] 3) The material collected in step 2) is subjected to low-temperature crystallization. After crystallization is completed, solid-liquid separation is performed to collect the liquid phase component. The conditions for low-temperature crystallization are: temperature -20℃ and time 10 hours. The solid-liquid separation method is vacuum filtration. The conditions for vacuum filtration are: medium-speed qualitative filter paper with a pore size of 10μm and filtration pressure of 0.08MPa.

[0064] 4) Perform molecular distillation on the liquid phase component obtained in step 3), collect the distillate, and obtain nervonic acid; the conditions for molecular distillation are: pressure of 1.5 Pa and evaporation surface temperature of 165 °C.

[0065] The preparation method for decaffeinated coffee includes the following steps:

[0066] 1) Grind and dry the coffee beans to obtain coffee powder;

[0067] 2) The coffee powder from step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 20 MPa, the separation temperature is 58°C, the extraction temperature is 50°C, and the extraction time is 1.5 hours.

[0068] 3) Collect the solid material in the extraction vessel from step 2) to obtain decaffeinated coffee.

[0069] The coffee composition is prepared by the following steps: uniformly mixing ginseng polypeptide, nervonic acid and decaffeinated coffee to form the coffee composition.

[0070] Example 2

[0071] Model preparation:

[0072] Forty healthy male Balb / c mice were selected and randomly divided into two groups: a control group and a model group. The model group received a subcutaneous injection of D-galactose solution (500 mg / kg / day) into the neck and back, while the control group received the same volume of sterile saline (NS) subcutaneously into the neck and back. Mice were weighed and the injection dosage adjusted every other day, and the modeling process continued for 6 weeks. All mice were fed and hydrated normally, and the room temperature was (24±2)℃. After successful modeling, the model group was further divided into three groups: a model group, a positive control group, and a composition group. The positive control group (vitamin E, 100 mg / kg / day) and the composition group (coffee composition prepared in Example 1, 500 mg / kg / day) were administered by gavage for 4 weeks, while the model group and the control group received the same volume of NS.

[0073] Indicator Testing:

[0074] 1. Morris Water Maze Test

[0075] Following the last administration, mice underwent a water maze test. The Morris water maze test included a concealed platform test and a spatial exploration test. The circular maze pool had a diameter of 0.67 m and a height of 0.25 m, and was divided into four quadrants. A circular safety platform with a diameter of 15 cm was placed in the center of the first quadrant, with water submerging the platform by 1 cm. The water temperature in the maze pool was controlled within the range of (25 ± 0.5) ℃.

[0076] Hidden Platform Test: Before the formal test, each mouse was trained twice daily for 60 seconds each time, and was placed into the water maze from different points (four quadrants) for a total of 4 days. During training, mice that failed to find the platform were guided, and the escape latency of each mouse was recorded. The learning and memory abilities of the mice were assessed by recording the total time from entering the water to finding the platform.

[0077] Spatial exploration test: The platform was removed the day after the concealed platform test, and a 60-second spatial exploration test began. The time each mouse spent on the platform in the first quadrant and the number of times it crossed this quadrant were recorded. The maze test lasted for 6 days. After each test, the wet mice were dried with warm air before being returned to their cages.

[0078] 2. Novel object recognition test (NOR)

[0079] The new object recognition experiment is divided into three periods: familiarization period, training period, and testing period. ① Familiarization period (T0): A few days before the experiment, the mice are petted (for at least 1 minute each time) to reduce their unfamiliarity with the test subject. 24 hours before the experiment or training, the mice are placed in a 50cm cube-shaped experimental box and allowed to explore freely for 5 minutes to reduce their unfamiliarity with the testing environment. ② Training period (T1): Two identical objects, A1 and A2, are placed in opposite corners of the experimental box (northeast and southwest corners). The mice are allowed to explore freely for 5 minutes, and the total exploration time for the two identical objects is recorded. ③ Testing period (T2): Objects A1 or A2 used in T1 and a novel object B are placed in opposite corners of the experimental box, allowing free exploration for 5 minutes. The total exploration time for the new and old objects is recorded. The discrimination index (DI) is used as an indicator of learning and memory. Throughout the new object recognition experiment, the experimental objects were cleaned with 75% ethanol disinfectant to eliminate the odor of each experimental mouse and avoid interference with the experiment (a recognition response is indicated when the mouse faces the object and the distance between its nose and the object is ≤2cm).

[0080] 3. Detection of SOD and GSH-Px activity and MDA content in hippocampal tissue

[0081] The concentrations of SOD, GSH-Px, and MDA in hippocampal tissue were determined using ELISA, following the instructions in the kit manual.

[0082] 4. Western Blot analysis of the hippocampus in brain tissue

[0083] (I) Processing of hippocampal samples and determination of protein content

[0084] Preparation of samples for hippocampal tissue protein expression experiment: At the end of the experiment, mice were euthanized and their hippocampal tissue was carefully obtained and cut into very fine fragments. Cytoplasmic protein extraction reagents A and B were mixed at a ratio of 20:1. Then, lysis buffer was added at a ratio of 1:5, and the mixture was homogenized in an ice bath. After standing for 15 minutes, it was centrifuged at 2500 rpm for 5 minutes at low temperature. The supernatant was transferred to pre-chilled EP tubes, which are the hippocampal tissue proteins.

[0085] BCA method for determining hippocampal protein content: The protein content of hippocampal tissue was determined according to the instructions of the BCA protein assay kit.

[0086] (II) Sample Processing

[0087] Add the protein solution to 5×Loding buffer at a ratio of 4:1, denature in a boiling water bath for 15 minutes, and store at -20°C for later use.

[0088] (III) Electrophoresis

[0089] After preparing the stacking and separating gels, begin electrophoresis. Use 75V for the stacking gel and 120V for the separating gel. Stop electrophoresis when the bromophenol blue is approximately 1cm from the bottom.

[0090] (iv) Transfer membrane

[0091] After the activated filter paper, PVDF membrane and sponge pad are stacked into a "sandwich" structure, the transfer process begins, and the membrane is transferred at a constant current of 300mA in an ice bath for half an hour.

[0092] (V) Immune Response

[0093] Perform the incubation process of primary antibody, secondary antibody, and internal control in sequence according to the antibody instructions.

[0094] (vi) Chemiluminescence

[0095] Darkroom exposure was performed using a developing and fixing kit and an ECL chemiluminescence kit.

[0096] (vii) Gel Image Analysis

[0097] The imaging film was scanned and archived, then processed and desaturated using Photoshop, and its grayscale values ​​were analyzed using the Alpha software processing system.

[0098] Results and Analysis:

[0099] (1) Results of the Morris water maze experiment

[0100] (I) Results of Covert Platform Tests

[0101] Table 1 reflects the changes in escape latency of mice in the control group, model group, positive control group, and combined group during the 5-day hidden platform exploration test. The escape latency of each group decreased with increasing training time, with the control group showing the fastest decrease and the model group the slowest. From day 2 to day 5, compared with the control group, mice in the model group spent more time searching for the hidden platform each day, suggesting a significant decline in their learning and memory abilities. Compared with the model group, on day 5 of the hidden platform test, the escape latency was 30.33±2.21s in the control group, 43.25±3.74s in the model group, 33.80±3.64s in the positive control group, and 28.35±2.91s in the combined group. The data show that the escape latency of mice in the combined group and positive control group was significantly reduced compared with the model group (P<0.05).

[0102] Table 1. Changes in the 5-day escape latency of mice in the experimental group.

[0103]

[0104] Note: Compared with the model group, #P<0.05, ##P<0.01; compared with the treatment group, *P<0.05, **P<0.01.

[0105] (II) Space Exploration Test Results

[0106] After the hiding platform was removed, the swimming trajectories of the model group mice became disordered, mainly swimming around the pool wall and randomly distributed in the four quadrants. This indicates that the model group mice did not accurately remember the original platform's location, suggesting a significant decline in their spatial memory ability. The data results are shown in Table 2. The control group mice spent 26.50±2.22s in the original platform quadrant, significantly longer than the model group (21.86±4.94s). After drug intervention, the VE and combination group mice primarily searched in the original platform quadrant and the adjacent quadrants to the left and right of it, spending 26.80±2.56s and 29.88±3.48s respectively in the original platform quadrant, significantly longer than the model group (P<0.05). Furthermore, the number of times the model group crossed the original platform was 1.71±0.70s, significantly lower than the 2.83±0.69s in the control group; while VE and the combination significantly increased the number of times the mice crossed the original platform, at 2.20±0.75s and 2.88±0.60s respectively, significantly higher than the model group (P<0.05). This indicates that the combination intervention can significantly improve the learning and memory abilities of the model mice (see Table 2).

[0107] Table 2 Results of spatial exploration tests in experimental mice

[0108]

[0109] Note: Compared with the model group, #P<0.05, ##P<0.01; compared with the treatment group, *P<0.05, **P<0.01.

[0110] (2) Results of the new object recognition experiment

[0111] Novel object recognition memory can be used to evaluate hippocampal-dependent non-spatial learning and memory abilities in mice. According to Figure 1 In the control group (A), the total exploration time was 67.5 ± 4.11 s, significantly higher than that in the model group (51.43 ± 3.77 s). However, treatment with the combination or vitamin E significantly increased the total exploration time for both new and old objects, to 69.88 ± 2.80 s and 68.40 ± 2.42 s, respectively. Figure 1 (A). Meanwhile, its NOR is as follows: Figure 1As shown in Figure B, the NOR in the model group (0.64±0.023) was significantly lower than that in the control group (0.86±0.030), while the NOR in mice treated with the combination or vitamin E significantly increased to 0.83±0.029 and 0.83±0.029, respectively. Figure 1 (B). Therefore, the composition can significantly improve the ability of mice to recognize new objects.

[0112] (3) The composition reduces oxidative stress.

[0113] The oxidative stress response of the composition to mouse serum and brain tissue was evaluated by measuring the activities of SOD and GSH-Px and the content of MDA in mouse serum and hippocampal tissue. Compared with the control group, the activities of SOD and GSH-Px in the serum and brain tissue of the model group mice were significantly decreased (P<0.05), while the MDA level was significantly increased (P<0.05). After treatment, the contents of SOD and GSH-Px in the serum and brain tissue of both the composition group and the positive control group were significantly restored. In addition, compared with the model group, the MDA content was significantly decreased after treatment with the composition and vitamin E, indicating that the composition has a protective effect on mouse serum and brain tissue (see...). Figure 2 China A and Figure 2 (B)

[0114] (4) The composition activates the Nrf2 / HO-1 signaling pathway

[0115] This study analyzed the effect of the composition on the Nrf2 / HO-1 signaling pathway by detecting the expression levels of Nrf2, HO-1 and NQO1 in the hippocampus of mouse brain tissue. Figure 3 Immunoblot analysis showed that D-galactose significantly inhibited the protein expression of Nrf2 and HO-1, while drug treatment reversed the inhibition of Nrf2 and related protein expression in the hippocampus by D-galactose. Therefore, the composition can alleviate oxidative stress-induced memory loss by activating the Nrf2 / HO-1 signaling pathway.

[0116] In summary, this composition can reduce oxidative stress in the hippocampus and improve non-spatial learning and memory abilities by activating the Nrf2 / HO-1 signaling pathway.

[0117] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A coffee composition with memory-improving function, characterized in that, It includes the following components in parts by weight: 3 parts of ginseng polypeptide, 1 part of nervonic acid, and 16 parts of decaffeinated caffeine.

2. The coffee composition according to claim 1, characterized in that, The preparation method of the ginseng polypeptide includes the following steps: 1) Mix the ginseng powder with an ethanol solution and extract by ultrasonication. After centrifugation, filter the supernatant under reduced pressure to obtain the filtrate. 2) Pass the filtrate obtained in step 1) through an AB-8 macroporous resin column and elute with water, 5% ethanol solution, 15% ethanol solution and 25% ethanol solution, respectively, for 2 hours, 2 hours, 3 hours and 4 hours. After mixing the solutions eluted with ethanol solution, dry at 40°C to constant weight to obtain ginseng polypeptide.

3. The coffee composition according to claim 2, characterized in that, In step 1), the ratio of the ginseng powder to the ethanol solution is 1:10 g / mL. The volume percentage of the ethanol solution is 65%; The conditions for ultrasonic extraction include: ultrasonic power of 350W and extraction time of 35 minutes; The centrifugation conditions include: centrifugation at 3000 rpm for 10 minutes; The conditions for the reduced pressure filtration include: medium-speed qualitative filter paper with a pore size of 10 μm and a filtration pressure of 0.08 MPa.

4. The coffee composition according to claim 1, characterized in that, The method for preparing the nervonic acid includes the following steps: 1) Crush and dry the seeds of Acer truncatum to obtain Acer truncatum seed powder; 2) The Acer truncatum seed powder described in step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 32 MPa, the separation temperature is 58°C, the extraction temperature is 45°C, and the extraction time is 1.5 hours. The material in the separation vessel is then collected. 3) The material collected in step 2) is subjected to low-temperature crystallization. After crystallization is completed, solid-liquid separation is performed, and the liquid phase component is collected. 4) Perform molecular distillation on the liquid phase component obtained in step 3), collect the distillate, and obtain nervonic acid.

5. The coffee composition according to claim 4, characterized in that, The conditions for low-temperature crystallization in step 3) are: temperature -20℃ and time 10 hours; The solid-liquid separation method is vacuum filtration, and the conditions for vacuum filtration include: medium-speed qualitative filter paper with a pore size of 10 μm and a filtration pressure of 0.08 MPa.

6. The coffee composition according to claim 4, characterized in that, The conditions for molecular distillation in step 4) are: pressure of 1.5 Pa and evaporation surface temperature of 165 °C.

7. The coffee composition according to claim 1, characterized in that, The method for preparing the decaffeinated coffee includes the following steps: 1) Grind and dry the coffee beans to obtain coffee powder; 2) The coffee powder described in step 1) is loaded into a supercritical CO2 fluid extraction vessel. The extraction pressure is 20 MPa, the separation temperature is 58°C, the extraction temperature is 50°C, and the extraction time is 1.5 hours. 3) Collect the solid material in the extraction vessel from step 2) to obtain decaffeinated coffee.

8. A method for preparing the coffee composition according to any one of claims 1 to 7, characterized in that, Includes the following steps: The ginseng polypeptide, nervonic acid, and decaffeinated coffee are uniformly mixed to form a coffee composition.

9. The use of the coffee composition of claim 1 in the preparation of products for relieving memory decline and assisting in improving memory.

10. The application according to claim 9, characterized in that, The product in question is food, medicine, or health supplement.

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