Fermented fertilizer containing pig manure and preparation method thereof

Abstract

The invention discloses a fermented fertilizer containing pig manure and a preparation method thereof, and belongs to the technical field of fertilizer preparation. The invention provides a preparation method of a fermented fertilizer containing pig manure, which comprises the following steps: crushing pepper branches, honeysuckle branches and cherry branches until the length is not more than 3cm, drying in the sun, and mixing with pig manure to obtain a fermentation substrate; mixing the fermentation substrate with a compound enzyme, adding water to adjust the water content of the mixed material to 30-40%, and stirring and fermenting for 5-7 days to obtain a primary fermentation product; and mixing the primary fermentation product, zeolite powder and a complex microbial inoculant, carrying out stacking fermentation, turning the stack for 1-3 times every day, and fermenting for 23-25 days to obtain the organic fertilizer, the compound enzyme is cellulase, pectinase and urease; the complex microbial inoculants are acinetobacter calcoaceticus, brevibacillus laterosporus and candida krusei. The fermented fertilizer provided by the invention can effectively improve the tolerance of plants to salt stress and improve various growth indexes of the plants.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of fertilizer preparation, and relates to a fermented fertilizer containing pig manure and a preparation method thereof. BACKGROUND

[0002] With the development of modern intensive agriculture and salt accumulation in irrigated areas, soil salinization has become a global problem that restricts crop productivity. Salt stress significantly inhibits plant growth through mechanisms such as osmotic stress, ion toxicity, and oxidative damage, leading to decreased biomass, metabolic disorders, and even death. Currently, conventional methods to alleviate salt stress (such as the use of amendments, the breeding of salt-tolerant varieties, etc.) have limitations such as high cost, long cycle, or unstable effects. Although organic fertilizers can improve soil structure, their physiological protective effects on plants in saline environments are limited, especially their lack of targeted ability to enhance the antioxidant system.

[0003] Existing fermented fertilizers containing livestock and poultry manure focus on nutrient supply, but ignore the enhancement of stress resistance under salt stress. For example, in traditional composting processes, the degradation efficiency of lignocellulosic raw materials (such as tree branches) is low, limiting the conversion of organic matter; the compatibility of microbial agents is single, making it difficult to synergistically regulate the osmotic balance and oxidative stress response of plants. In addition, the enrichment of sodium ions in saline soils can easily damage the stability of the cell membrane, and conventional fertilizers lack mechanisms to alleviate this. Therefore, there is an urgent need to develop a functional fertilizer that can efficiently degrade agricultural waste, activate plant salt-tolerant physiological pathways, and adapt to saline soils, in order to achieve the dual goals of resource recycling and stress resistance cultivation. SUMMARY

[0004] The present application aims to develop a fermented fertilizer containing pig manure to improve the tolerance of plants to salt stress.

[0005] In one aspect, the present application relates to a method for preparing a fermented fertilizer containing pig manure, which comprises: crushing pepper branches, honeysuckle branches, and cherry branches to a length of not more than 3 cm, then drying, and then mixing with pig manure as a fermentation substrate; Mixing the fermentation substrate and a composite enzyme, adding water to adjust the moisture content of the mixture to 30-40%, and stirring and fermenting for 5-7 days to obtain a primary fermentation product; Mixing the primary fermentation product, zeolite powder, and a composite microbial agent, and then stacking and fermenting, with turning 1-3 times per day, for 23-25 days to obtain a fermented fertilizer containing pig manure; The composite enzyme is cellulase, pectinase, and urease; The composite microbial agent is Acinetobacter calcoaceticus, Brevibacillus laterosporus, and Kluyveromyces marxianus.

[0006] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the ratio of the pepper branches, honeysuckle branches, cherry branches and pig manure in the fermentation substrate is 1-2:1-2:1-2:1 by mass ratio.

[0007] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the addition amount of the compound enzyme is 0.05-0.1% by mass percentage.

[0008] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the ratio of the cellulase, pectinase and urease in the compound enzyme is 1-3:1-3:1 by mass ratio.

[0009] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the addition amount of the compound microbial agent is 0.1-0.3% of the primary fermentation product by mass percentage.

[0010] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the ratio of the Acinetobacter calcoaceticus, Brevibacillus laterosporus and Candida krusei in the compound microbial agent is 1:2-4:1 by cfu / mL.

[0011] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the addition amount of the zeolite powder is 3-8% of the primary fermentation product by mass percentage.

[0012] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the particle size of the zeolite powder is not higher than 300 mesh.

[0013] Further, in the preparation method of the fermented fertilizer containing pig manure provided by the application, the stirring fermentation is stirring for 10-15 min every 2-4 h, and the temperature is 50-65℃. The water content is maintained at 30-35% during the stacking fermentation.

[0014] On the other hand, the application relates to a fermented fertilizer containing pig manure, which is prepared by the preparation method of the fermented fertilizer containing pig manure.

[0015] Compared with the prior art, the technical scheme provided by the application has at least the following beneficial effects or advantages: The present application aims at the key limiting factor of plant growth in saline soil, and develops a pig manure fermentation fertilizer with agricultural waste resource and efficient salt resistance function through multi-component synergy and segmented process. First, the pepper branches, honeysuckle branches and cherry branches are selected to form the fermentation substrate with the pig manure, and the natural salt-resistant active substances in the pepper branches are used to pre-activate the plant stress resistance pathway; then the complex enzyme is used to efficiently break down the lignocellulose structure at 50-65 DEG C stirring fermentation stage, in which the pectinase specifically degrades the pectin in the intercellular layer of the branches to release oligosaccharide signal molecules to induce plant immune response, and the urease precisely regulates the nitrogen release to solve the problem of low organic matter conversion rate in traditional composting; then the complex microbial agent and 300 mesh ultrafine zeolite powder are introduced to realize the triple bio-mineral synergy in the stacking fermentation stage, the calcium acetate acinetobacter secretes compatible solutes to directly enhance the plant cell osmotic regulation ability, the brefeldin-resistant short-spore bacillus synthesizes antibacterial lipopeptide and auxin to promote root development, the candida tropicalis maintains the fermentation microecological balance and antagonizes soil-borne pathogens, and the porous structure of the zeolite selectively adsorbs Na + and slowly releases K + / Ca 2+ , significantly reduces the ion toxicity and membrane lipid damage. In summary, the present application precisely degrades the lignocellulose in the pepper, honeysuckle and cherry branches by using the complex enzyme, and realizes the efficient conversion of agricultural waste through the segmented process of "stirring fermentation + stacking fermentation" by combining the synergistic effect of the complex microbial agent, in which the calcium acetate acinetobacter enhances the plant osmotic regulation, the brefeldin-resistant short-spore bacillus promotes the root growth, the candida tropicalis maintains the microbial balance, and the zeolite powder adsorbs the sodium ions in the saline soil to relieve the ion toxicity. The fertilizer significantly improves the plant salt tolerance, and under the stress of 150 mmol / L NaCl, the tomato plant height, biomass and antioxidant enzyme activity are significantly improved, and the membrane lipid peroxidation product MDA content is reduced. The core advantage is to deeply integrate the waste resource and the activation of the plant salt resistance physiological pathway, which provides an efficient solution for the saline soil treatment. DETAILED DESCRIPTION

[0016] In the following, the technical solutions of the present application will be described in conjunction with the examples. However, the present application is not limited to the following examples. The experimental methods and detection methods described in each example are conventional methods unless otherwise specified. The reagents and materials described are commercially available unless otherwise specified.

[0017] In the following examples, the pepper branches, honeysuckle branches and cherry branches are obtained by collecting and removing the leaves, the variety of pepper is Yangling 8819, the variety of honeysuckle is Jinhua No. 3, and the variety of cherry is Jimi.

[0018] In the following examples, cellulase, pectinase, and urease were all obtained commercially. Cellulase (FDG-2225) was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., pectinase (FDY-2212) was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., and urease (CAS: 9002-13-5) was purchased from Guangdong Fangxin Biotechnology Co., Ltd., and the enzyme activity of each was not less than 60,000 U / g.

[0019] In the following examples, *Acinetobacter calcareae*, *Bacillus laterosporus*, and *Candida krusei* were all commercially available. *Acinetobacter calcareae* (ATCC 23055) was purchased from Shanghai Jihe Biotechnology Co., Ltd., *Bacillus laterosporus* (ATCC 64) from Shunyou (Shanghai) Biotechnology Co., Ltd., and *Candida krusei* (ATCC 14243) from Shanghai Fuxiang Biotechnology Co., Ltd. Before use, the mixture was reconstituted with an appropriate amount of water and brought to a final volume of 50 mL. The effective viable count of each of the *Acinetobacter calcareae*, *Bacillus laterosporus*, and *Candida krusei* was determined to be 1 × 10⁻⁶. 9 cfu / mL.

[0020] Example 1 This embodiment provides a process for preparing fermented fertilizer containing pig manure.

[0021] S1. Raw material processing: Collect fresh chili pepper branches, honeysuckle branches, and cherry branches, crush them into branches no longer than 3cm using a pulverizer, and sun-dry them to obtain chili pepper branches, honeysuckle branches, and cherry branches for later use; grind zeolite into powder and sieve it through a 300-mesh sieve to obtain zeolite powder for later use; mix the collected pig manure and perform solid-liquid separation to remove excess liquid and obtain pig manure with a moisture content of 40% for later use.

[0022] S2. Primary fermentation: Chili pepper branches, honeysuckle branches, cherry branches, and pig manure were mixed in a mass ratio of 1:1:1:1 and used as the fermentation substrate. Cellulase, pectinase, and urease were mixed in a mass ratio of 1:1:1 as a compound enzyme. The compound enzyme was added to the fermentation substrate at a dosage of 0.05%, and an appropriate amount of water was added and mixed evenly. Finally, water was added until the moisture content of the mixture was 30%. The mixture was stirred and fermented at a temperature of 50℃ for 10 minutes every 2 hours. The primary fermentation product was obtained after 5 days of stirring and fermentation.

[0023] S3. Secondary Fermentation: *Acinetobacter calcareosa*, *Bacillus laterosporus*, and *Candida krusei* were used as a compound inoculum at a CFU / mL ratio of 1:2:1. The compound inoculum was added to the primary fermentation product at a concentration of 0.1%, and zeolite powder was added to the primary fermentation product at a concentration of 3%. After thorough mixing, the mixture was stacked (trapezoidal shape, 1.2m high, 1m³ in volume). 3Before fermentation, the moisture content should be controlled to be no higher than 30%. The pile should be turned over once a day, and the moisture content should be checked to be about 30% each time. The product is obtained after 25 days of fermentation.

[0024] Example 2 This embodiment provides a process for preparing fermented fertilizer containing pig manure.

[0025] S1. Raw material processing: Collect fresh chili pepper branches, honeysuckle branches, and cherry branches, crush them into branches no longer than 3cm using a pulverizer, and sun-dry them to obtain chili pepper branches, honeysuckle branches, and cherry branches for later use; grind zeolite into powder and sieve it through a 300-mesh sieve to obtain zeolite powder for later use; mix the collected pig manure and perform solid-liquid separation to remove excess liquid and obtain pig manure with a moisture content of 40% for later use.

[0026] S2. Primary fermentation: Chili pepper branches, honeysuckle branches, cherry branches, and pig manure were mixed in a mass ratio of 1:2:1:1 and used as the fermentation substrate. Cellulase, pectinase, and urease were mixed in a mass ratio of 2:2:1 as a compound enzyme. The compound enzyme was added to the fermentation substrate at a dosage of 0.06%, and an appropriate amount of water was added and mixed evenly. Finally, water was added until the moisture content of the mixture was 35%. The mixture was stirred and fermented at a temperature of 60℃ for 12 minutes every 3 hours. The primary fermentation product was obtained after 6 days of stirring and fermentation.

[0027] S3. Secondary Fermentation: *Acinetobacter calcareae*, *Bacillus laterosporus*, and *Candida krusei* were used as a compound inoculum at a CFU / mL ratio of 1:3:1. The compound inoculum was added to the primary fermentation product at a concentration of 0.2%, and zeolite powder was added at a concentration of 5%. After thorough mixing, the mixture was stacked (trapezoidal shape, 1.2m high, 1m³ in volume). 3 Before fermentation, the moisture content should be controlled to be no higher than 30%. The pile should be turned over twice a day, and the moisture content should be checked to be about 30% each time. The product is obtained after 24 days of fermentation.

[0028] Example 3 This embodiment provides a process for preparing fermented fertilizer containing pig manure.

[0029] S1. Raw material processing: Collect fresh chili pepper branches, honeysuckle branches, and cherry branches, crush them into branches no longer than 3cm using a pulverizer, and sun-dry them to obtain chili pepper branches, honeysuckle branches, and cherry branches for later use; grind zeolite into powder and sieve it through a 300-mesh sieve to obtain zeolite powder for later use; mix the collected pig manure and perform solid-liquid separation to remove excess liquid and obtain pig manure with a moisture content of 40% for later use.

[0030] S2. Primary fermentation: Chili pepper branches, honeysuckle branches, cherry branches, and pig manure were mixed in a mass ratio of 1:2:2:1 and used as the fermentation substrate. Cellulase, pectinase, and urease were mixed in a mass ratio of 3:2:1 as a compound enzyme. The compound enzyme was added to the fermentation substrate at a dosage of 0.08%, and an appropriate amount of water was added and mixed evenly. Finally, water was added until the moisture content of the mixture was 35%. The mixture was stirred and fermented at a temperature of 60℃ for 12 minutes every 3 hours. The primary fermentation product was obtained after 6 days of stirring and fermentation.

[0031] S3. Secondary Fermentation: *Acinetobacter calcareae*, *Bacillus laterosporus*, and *Candida krusei* were used as a compound inoculum at a CFU / mL ratio of 1:3:1. The compound inoculum was added to the primary fermentation product at a concentration of 0.2%, and zeolite powder was added at a concentration of 5%. After thorough mixing, the mixture was stacked (trapezoidal shape, 1.2m high, 1m³ in volume). 3 Before fermentation, the moisture content should be controlled to be no higher than 30%. The pile should be turned over twice a day, and the moisture content should be checked to be about 30% each time. The product is obtained after 24 days of fermentation.

[0032] Example 4 This embodiment provides a process for preparing fermented fertilizer containing pig manure.

[0033] S1. Raw material processing: Collect fresh chili pepper branches, honeysuckle branches, and cherry branches, crush them into branches no longer than 3cm using a pulverizer, and sun-dry them to obtain chili pepper branches, honeysuckle branches, and cherry branches for later use; grind zeolite into powder and sieve it through a 300-mesh sieve to obtain zeolite powder for later use; mix the collected pig manure and perform solid-liquid separation to remove excess liquid and obtain pig manure with a moisture content of 40% for later use.

[0034] S2. Primary fermentation: Chili pepper branches, honeysuckle branches, cherry branches, and pig manure were mixed in a mass ratio of 2:2:2:1 and used as the fermentation substrate. Cellulase, pectinase, and urease were mixed in a mass ratio of 3:3:1 as a compound enzyme. The compound enzyme was added to the fermentation substrate at a dosage of 0.1%, and an appropriate amount of water was added and mixed evenly. Finally, water was added until the moisture content of the mixture was 40%. The mixture was stirred and fermented at a temperature of 65℃ for 15 minutes every 4 hours. The primary fermentation product was obtained after 7 days of stirring and fermentation.

[0035] S3. Secondary Fermentation: *Acinetobacter calcareae*, *Bacillus laterosporus*, and *Candida krusei* were used as a compound inoculum at a CFU / mL ratio of 1:4:1. The compound inoculum was added to the primary fermentation product at a concentration of 0.3%, and zeolite powder was added to the primary fermentation product at a concentration of 8%. After thorough mixing, the mixture was stacked (trapezoidal shape, 1.2m high, 1m³ in volume). 3Before fermentation, the moisture content should be controlled to be no higher than 35%. The pile should be turned over 3 times a day, and the moisture content should be checked to be about 35% each time. The product is obtained after 23 days of fermentation.

[0036] Comparative Example 1 This comparative example is the same as Example 2, except that the fermentation substrate does not contain chili pepper branches.

[0037] Comparative Example 2 This comparative example is the same as Example 2, except that the complex enzyme does not contain pectinase.

[0038] Comparative Example 3 This comparative example is the same as Example 2, except that the compound bacterial agent does not contain Acinetobacter calcium acetate.

[0039] Comparative Example 4 This comparative example is the same as Example 2, except that the compound bacterial agent does not contain Candida krusei.

[0040] Example 5 This embodiment provides a pot experiment demonstrating how the above-mentioned fertilizer promotes salt-tolerant plant growth.

[0041] 72-cell seedling trays were used for seedling cultivation. When the tomato seedlings reached the three-leaf stage, seedlings of uniform growth were transplanted into pots containing peat moss. The pots, each with a 1L tray to prevent nutrient solution loss, contained 800mL of substrate. For the first 5 days after transplanting, the seedlings were watered daily with 50mL of 50% Hoagland's nutrient solution to help them recover. After the recovery period, the following treatments were administered: Blank control group: 100 mL of tap water was applied every 3 days.

[0042] Salt stress group: 100 mL of tap water was applied every 3 days, and 150 mmol / L NaCl solution was irrigated into the root system every 3 days.

[0043] Experimental Group 1: After the seedling establishment period, the fertilizer prepared in Example 1 was applied at a rate of 10g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100mL of tap water was applied every 3 days, and 150mmol / L NaCl solution was irrigated into the root system every 3 days.

[0044] Experimental Group 2: After the seedling establishment period, the fertilizer prepared in Example 2 was applied at a rate of 10g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100mL of tap water was applied every 3 days, and 150mmol / L NaCl solution was irrigated into the root system every 3 days.

[0045] Experimental Group 3: After the seedling establishment period, the fertilizer prepared in Example 3 was applied at a rate of 10g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100mL of tap water was applied every 3 days, and 150mmol / L NaCl solution was irrigated into the root system every 3 days.

[0046] Experimental Group 4: After the seedling establishment period, the fertilizer prepared in Example 4 was applied at a rate of 10 g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100 mL of tap water was applied every 3 days, and 150 mmol / L NaCl solution was irrigated into the root system every 3 days.

[0047] Control group 1: After the seedling establishment period, the fertilizer prepared in control group 1 was applied at a rate of 10 g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100 mL of tap water was applied every 3 days, and 150 mmol / L NaCl solution was irrigated into the root system every 3 days.

[0048] Control Group 2: After the seedling establishment period, the fertilizer prepared in Comparative Example 2 was applied at a rate of 10 g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100 mL of tap water was applied every 3 days, and 150 mmol / L NaCl solution was irrigated into the root system every 3 days.

[0049] Control Group 3: After the seedling establishment period, the fertilizer prepared in Comparative Example 3 was applied at a rate of 10 g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100 mL of tap water was applied every 3 days, and 150 mmol / L NaCl solution was irrigated into the root system every 3 days.

[0050] Control group 4: After the seedling establishment period, the fertilizer prepared in control group 4 was applied at a rate of 10 g / kg. The fertilizer was applied when the first application of tap water was mixed with tap water and applied together. 100 mL of tap water was applied every 3 days, and 150 mmol / L NaCl solution was irrigated into the root system every 3 days.

[0051] After 35 days of cultivation in an incubator, the growth indicators and salt stress-related indicators of tomatoes were observed, recorded, and measured. The salt stress-related indicators were measured using commercially available kits. The results of the growth indicators are shown in Table 1, and the results of the salt stress-related indicators are shown in Table 2.

[0052] Table 1: Results of Growth Indicators Measurement

[0053] Table 2: Results of Salt Stress Tolerance Related Indicators

[0054] As shown in Tables 1 and 2, under 150 mmol / L NaCl stress, the tomato plant height, root length, and aboveground and underground fresh weight of the experimental groups treated with the fertilizers from each example were significantly better than those of the control group subjected to salt stress alone. Some indicators (such as plant height and aboveground fresh weight in experimental group 3) even exceeded those of the normally growing blank control group, indicating that the fertilizer not only effectively alleviated the inhibitory effect of salt stress on plant growth but also enhanced overall biomass accumulation. Regarding salt tolerance physiological indicators, each experimental group significantly reduced the content of malondialdehyde (MDA), a membrane lipid peroxidation product induced by salt stress (e.g., the aboveground MDA content in experimental group 3 was 30.51 nmol / g, significantly lower than the 53.61 nmol / g in the salt stress group), while maintaining or increasing the activities of antioxidant enzymes CAT (catalase) and SOD (superoxide dismutase) (e.g., the aboveground CAT content in experimental group 3 reached 50.19 U / g, higher than the 25.54 U / g in the salt stress group). This indicates that the fertilizer mitigated oxidative damage caused by salt stress by enhancing the plant's antioxidant capacity. Comparative experiments further validated the crucial role of the fertilizer formulation. The absence of pepper branches (control group 1) or specific microbial strains in the compound microbial agent (control groups 3 and 4) led to a significant decrease in plant growth indicators and antioxidant capacity (e.g., the underground fresh weight of control group 1 was only 3.60g, significantly lower than the 7.51g of experimental group 2; the aboveground CAT activity of control group 3 decreased to 37.82 U / g); while the lack of pectinase (control group 2) exacerbated MDA accumulation (44.86 nmol / g in the aboveground parts). The fertilizer prepared in Example 3 (corresponding to experimental group 3) showed the best performance in promoting biomass accumulation (plant height 25.02cm, aboveground fresh weight 35.43g) and maintaining the balance of the antioxidant system (lowest MDA, highest CAT). In summary, this fermented fertilizer effectively improved the salt tolerance and overall growth performance of tomatoes by optimizing the synergistic effect of enzymatic hydrolysis and microbial community.

[0055] The embodiments described above are some, but not all, of the embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art through related deductions and substitutions based on the inventive concept, without inventive effort, are within the scope of protection of the present invention.

Claims

1. A method for preparing a fermented fertilizer containing pig manure, characterized in that, include: Chili pepper branches, honeysuckle branches, and cherry branches are crushed into pieces no longer than 3cm, dried in the sun, and then mixed with pig manure as a fermentation substrate. The fermentation substrate and the compound enzyme are mixed, water is added to adjust the moisture content of the mixture to 30-40%, and the mixture is stirred and fermented for 5-7 days to obtain the primary fermentation product. The primary fermentation product, zeolite powder and compound microbial agent are mixed and then piled up for fermentation. The pile is turned over 1 to 3 times a day and fermented for 23 to 25 days to obtain the product. The complex enzyme is cellulase, pectinase, and urease; The compound microbial agent consists of Acinetobacter calcium acetate, Bacillus lateralis, and Candida krusei.

2. The method for preparing fermented fertilizer containing pig manure according to claim 1, characterized in that, By mass ratio, the ratio of chili branches, honeysuckle branches, cherry branches and pig manure in the fermentation substrate is 1~2:1~2:1~2:

1.

3. The method for preparing fermented fertilizer containing pig manure according to claim 1, characterized in that, The amount of the compound enzyme added is 0.05~0.1% by mass percentage.

4. The method for preparing fermented fertilizer containing pig manure according to claim 3, characterized in that, The ratio of cellulase, pectinase and urease in the compound enzyme is 1~3:1~3:1 by mass.

5. The method for preparing fermented fertilizer containing pig manure according to claim 1, characterized in that, The amount of the compound microbial agent added is 0.1 to 0.3% of the primary fermentation product by mass percentage.

6. The method for preparing fermented fertilizer containing pig manure according to claim 5, characterized in that, The ratio of Acinetobacter calciacetate, Bacillus laterosporus, and Candida krusei in the compound bacterial agent is 1:2 to 4:1, calculated in CFU / mL.

7. The method for preparing fermented fertilizer containing pig manure according to claim 1, characterized in that, The amount of zeolite powder added is 3-8% of the primary fermentation product by mass percentage.

8. The method for preparing fermented fertilizer containing pig manure according to claim 7, characterized in that, The particle size of the zeolite powder is no higher than 300 mesh.

9. The method for preparing fermented fertilizer containing pig manure according to claim 1, characterized in that, The stirring fermentation is carried out by stirring for 10-15 minutes every 2-4 hours at a temperature of 50-65℃. The moisture content is maintained at 30-35% during the stacking fermentation process.

10. A fermented fertilizer containing pig manure, characterized in that, It is prepared by the method for preparing fermented fertilizer containing pig manure according to any one of claims 1 to 9.

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