Portable isothermal amplification detection equipment

Abstract

The invention relates to the technical field of nucleic acid detection, and particularly discloses portable isothermal amplification detection equipment which comprises a machine body, a hollow groove is formed in the machine body, an adjusting assembly is arranged in the hollow groove and used for lifting a sample, a heating assembly is arranged at the bottom of the hollow groove, and the adjusting assembly is connected with the heating assembly. The heating assembly is used for heating a sample, circulating assemblies are arranged at the two ends of the front side of the heating assembly and used for heat circulation, a lifting plate is driven to be lifted by opening a top cover, the top end of a jacking block makes contact with a first sliding plate at the moment, then the lifting plate is lifted, and a to-be-detected sample tube is put into the machine body at the moment; and the other end of the traction rope is limited by a pulley block, so that a connecting strip is pulled to descend, a supporting seat is driven to be separated from a reset spring to descend, heating is stopped, meanwhile, the whole lifting plate is lifted, a sample tube is lifted, and a worker can take the sample tube conveniently.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid detection technology, specifically a portable isothermal amplification detection device. Background Technology

[0002] Isothermal amplification nucleic acid detection instruments are clinical testing devices used for nucleic acid amplification experiments. They feature key functions such as temperature control, reaction tube loading, temperature uniformity, time control, and real-time monitoring. These functions help laboratory personnel perform efficient and accurate nucleic acid amplification experiments. Isothermal amplification nucleic acid detection instruments are widely used in clinical laboratories for various fields, including the detection of animal-derived components from meat, foodborne pathogens, genetically modified organisms, animal diseases, and scientific research. The main advantages of isothermal amplification nucleic acid detection instruments include high specificity, high fidelity, multi-channel operation, and isothermal amplification. The multi-channel design allows for the simultaneous detection of multiple target gene sequences, improving detection efficiency. However, in conventional isothermal amplification detection equipment, after the sample is heated, the sample tube is inside the base, making it inconvenient for staff to hold when removing the sample tube, easily leading to it falling out. Therefore, we propose a portable isothermal amplification detection device. Summary of the Invention

[0003] The purpose of this invention is to provide a portable isothermal amplification detection device to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a portable isothermal amplification detection device, comprising a body, a hollow groove inside the body, an adjustment component inside the hollow groove for lifting the sample, a heating component at the bottom of the hollow groove, the adjustment component and the heating component being connected to each other, the heating component for heating the sample, and circulation components at both ends of the front side of the heating component for heat circulation.

[0005] The adjustment assembly includes a lifting plate, a lifting plate, and a limiting plate. A lifting block is fixedly connected to the bottom front side of the lifting plate. A first sliding plate is fixedly connected to the left and right sides of the lifting plate. The lifting block is in contact with the first sliding plate. A spool is fixedly connected to the inner side of the lifting block. A traction rope is wound inside the spool. A heating assembly is slidably connected inside the limiting plate.

[0006] The heating component includes a support base, with several second sliding plates fixedly connected to both the left and right ends of the support base. Adjacent second sliding plates are connected by connecting strips. A heating plate is provided on the top of the support base, and reset springs are provided on both the left and right sides of the bottom of the support base. A circulation component is provided at the bottom of each of the front second sliding plates.

[0007] The circulation component includes a fixed base and a connecting rod. A fan is rotatably connected to the middle of the fixed base, and a connecting rod is fixedly connected to the other end of the connecting rod. The other end of the connecting rod is fixedly connected to the bottom of the fan.

[0008] The hollow trough is equipped with two pulley sets, located on the inner wall of the rear side and the inner wall of the bottom of the hollow trough, respectively. The traction rope is connected to the connecting strip through the pulley sets.

[0009] The machine body has several ventilation holes on the left and right sides of the internal support, a placement seat at the bottom of the lifting plate, and a temperature sensor on the front inner wall of the machine body.

[0010] The machine body has a control panel on the front, a display screen on the top, and several control buttons at the bottom.

[0011] The top of the machine body is rotatably connected to a top cover, and a latch is provided on the front side of the top cover. The machine body and the top cover are interlocked by the latch.

[0012] Anti-slip pads are fixedly attached to the four corners of the bottom of the machine.

[0013] This invention has at least the following beneficial effects:

[0014] By opening the top cover, the lifting plate is raised, at which point the top of the lifting block contacts the first sliding plate, thus raising the lifting plate. The sample tube to be tested is then placed into the machine body. Simultaneously, as the lifting plate rises, the spool rises synchronously, pulling the traction rope. The other end of the traction rope is restrained by a pulley system, pulling the connecting rod downwards and causing the support seat to disengage from the return spring, thus stopping heating. Simultaneously, the second sliding plate descends and presses down the connecting rod, causing its other end to retract, thereby pulling the connecting rod and adjusting the airflow direction of the fan to blow outwards. After the sample tube to be tested is placed, the top cover is closed, and the lifting plate descends, causing the spool to reset with the heating and circulation components, bringing the sample tube into the placement seat. It is then heated by the heating plate and circulated by the fan for amplification testing. The testing method, amplification temperature, and time can be adjusted via the control panel according to different needs. Attached Figure Description

[0015] Figure 1 This is a perspective view of the present invention;

[0016] Figure 2 This is a perspective view of the invention in its unfolded state;

[0017] Figure 3 This is a right sectional view of the present invention;

[0018] Figure 4 This is a first schematic diagram of the internal structure of the present invention;

[0019] Figure 5 This is a schematic diagram of the heating component of the present invention;

[0020] Figure 6 This is a schematic diagram of the regulating component and the circulation component of the present invention;

[0021] Figure 7 This is a second schematic diagram of the internal structure of the present invention.

[0022] In the diagram: 1. Body; 2. Top cover; 3. Lock; 4. Display screen; 5. Control panel; 6. Control button; 7. Hollow slot; 8. Adjustment component; 801. First sliding plate; 802. Lifting plate; 803. Lifting plate; 804. Ventilation hole; 805. Lifting block; 806. Limiting plate; 807. Pulley block; 808. Traction rope; 809. Spool; 810. Placement seat; 9. Anti-slip mat; 10. Heating component; 1001. Connecting strip; 1002. Second sliding plate; 1003. Return spring; 1004. Heating plate; 1005. Support seat; 11. Circulation component; 1101. Fixed seat; 1102. Connecting rod; 1103. Connecting rod; 1104. Fan; 12. Temperature sensor. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Please see Figures 1 to 7 :

[0025] Example 1

[0026] This invention provides a technical solution: a portable isothermal amplification detection device, including a body 1, a hollow groove 7 inside the body 1, an adjustment component 8 inside the hollow groove 7 for lifting the sample, a heating component 10 at the bottom of the hollow groove 7, the adjustment component 8 and the heating component 10 being connected to each other, the heating component 10 for heating the sample, and circulation components 11 at both ends of the front side of the heating component 10 for heat circulation;

[0027] The adjustment assembly 8 includes a lifting plate 802, a lifting plate 803, and a limiting plate 806. A lifting block 805 is fixedly connected to the bottom front side of the lifting plate 802. A first sliding plate 801 is fixedly connected to the left and right sides of the lifting plate 803. The lifting block 805 is in contact with the first sliding plate 801. A spool 809 is fixedly connected to the inner side of the lifting block 805. A traction rope 808 is wound inside the spool 809. A heating assembly 10 is slidably connected inside the limiting plate 806.

[0028] First, by opening the top cover 2, the lifting plate 802 is raised. At this time, the top of the lifting block 805 contacts the first sliding plate 801, thereby raising the lifting plate 803. The sample tube to be tested is then placed into the machine body 1. Simultaneously, as the lifting plate 802 rises, the spool 809 rises synchronously, thus pulling the traction rope 808. The other end of the traction rope 808 is restrained by the pulley group 807, thereby pulling the connecting bar 1001 down and causing the support base 1005 to disengage from the return spring 1003 and descend, thus stopping heating. Simultaneously, the second sliding plate 1002 descends... The pressure rod 1103 causes the other end to retract, thereby pulling the connecting rod 1102 and the fan 1104 to adjust the airflow direction, thus blowing outward. After the sample tube to be tested is placed, the top cover 2 is closed, and then the lifting plate 802 descends, driving the spool 809, heating component 10 and circulation component 11 to reset, and bringing the sample tube to be tested into the placement seat 810. It is heated by the heating plate 1004 and circulated by the fan 1104 for amplification detection. At the same time, the detection mode, amplification temperature and time can be adjusted by the control panel 5 according to different needs.

[0029] The heating assembly 10 includes a support base 1005. Several second sliding plates 1002 are fixedly connected to both ends of the support base 1005. The second sliding plates 1002 on adjacent sides are connected by connecting strips 1001. A heating plate 1004 is provided on the top of the support base 1005. Return springs 1003 are provided on both the left and right sides of the bottom of the support base 1005. A circulation assembly 11 is provided on the bottom of the front second sliding plate 1002. The support base 1005 is used to support the heating plate 1004 and drive the heating plate 1004 to rise and fall. The return springs 1003 are used to lift the support base 1005, thereby driving the support base 1005 to return to its original position, which facilitates subsequent heating.

[0030] The circulation assembly 11 includes a fixed base 1101 and a connecting rod 1103. A fan 1104 is rotatably connected to the middle of the fixed base 1101. A connecting rod 1102 is fixedly connected to the other end of the connecting rod 1103. The other end of the connecting rod 1102 is fixedly connected to the bottom of the fan 1104. The fan 1104 is used to provide airflow, guide heat circulation, and ensure heat balance. At the same time, the connecting rod 1102 is used to drive the fan 1104 to change its angle, so that when the top cover 2 is opened, the airflow direction can be reversed and blown upward to prevent ash and other substances from entering the body 1.

[0031] The hollow trough 7 has two pulley sets 807 inside, located on the rear inner wall and bottom inner wall of the hollow trough 7 respectively. The traction rope 808 is connected to the connecting bar 1001 through the pulley sets 807. The pulley sets 807 are used to ensure the stability of the winding traction of the traction rope 808 and to ensure the stability of the connecting bar 1001, so as to drive the support seat 1005 to sink and stop heating.

[0032] Example 2

[0033] Several ventilation holes 804 are provided on the left and right sides of the internal support of the machine body 1. A placement seat 810 is provided at the bottom of the lifting plate 803. A temperature sensor 12 is provided on the front inner wall of the machine body 1. The ventilation holes 804 are used for heat circulation to ensure temperature balance. The placement seat 810 is used to support and place the sample detection tube. The temperature sensor 12 is used to monitor the heating temperature to ensure the accuracy of the heating temperature.

[0034] A control panel 5 is provided on the front side of the machine body 1. A display screen 4 is provided on the top of the control panel 5. Several control buttons 6 are provided at the bottom of the display screen 4. The display screen 4 is used to display various data of the sample. The control buttons 6 are used to control the heating time, heating temperature, and number of cycles.

[0035] The top of the body 1 is rotatably connected to the top cover 2, and the front side of the top cover 2 is provided with a latch 3. The body 1 and the top cover 2 are interlocked by the latch 3.

[0036] Anti-slip pads 9 are fixedly connected to the four corners of the bottom of the body 1. The anti-slip pads 9 are used to ensure the stability of the body 1.

[0037] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0038] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A portable isothermal amplification detection device, comprising: The body (1) is characterized in that: a hollow groove (7) is provided inside the body (1), an adjustment component (8) is provided inside the hollow groove (7), the adjustment component (8) is used to lift the sample, a heating component (10) is provided at the bottom of the hollow groove (7), the adjustment component (8) and the heating component (10) are connected to each other, the heating component (10) is used to heat the sample, and a circulation component (11) is provided at both ends of the front side of the heating component (10), the circulation component (11) is used for heat circulation; The adjustment component (8) includes a lifting plate (802), a lifting plate (803), and a limiting plate (806). The front bottom of the lifting plate (802) is fixedly connected to a lifting block (805). The left and right sides of the lifting plate (803) are fixedly connected to a first sliding plate (801). The lifting block (805) is in contact with the first sliding plate (801). The inner side of the lifting block (805) is fixedly connected to a spool (809). The spool (809) has a traction rope (808) wound inside. The limiting plate (806) has a heating component (10) slidably connected inside.

2. The portable isothermal amplification detection device according to claim 1, characterized in that: The heating assembly (10) includes a support base (1005), and several second sliding plates (1002) are fixedly connected to both the left and right ends of the support base (1005). The second sliding plates (1002) on adjacent sides are connected by connecting strips (1001). A heating plate (1004) is provided on the top of the support base (1005). A return spring (1003) is provided on both the left and right sides of the bottom of the support base (1005). A circulation assembly (11) is provided at the bottom of the second sliding plate (1002) on the front side.

3. The portable isothermal amplification detection device according to claim 1, characterized in that: The circulation component (11) includes a fixed base (1101) and a connecting rod (1103). A fan (1104) is rotatably connected to the middle of the fixed base (1101). A connecting rod (1102) is fixedly connected to the other end of the connecting rod (1103). The other end of the connecting rod (1102) is fixedly connected to the bottom of the fan (1104).

4. The portable isothermal amplification detection device according to claim 1, characterized in that: The hollow groove (7) is provided with two pulley sets (807), located on the inner wall of the rear side and the inner wall of the bottom of the hollow groove (7), respectively. The traction rope (808) is connected to the connecting strip (1001) through the pulley sets (807).

5. The portable isothermal amplification detection device according to claim 1, characterized in that: The internal support of the body (1) has several ventilation holes (804) on the left and right sides, the bottom of the lifting plate (803) is provided with a placement seat (810), and the front inner wall of the body (1) is provided with a temperature sensor (12).

6. The portable isothermal amplification detection device according to claim 1, characterized in that: The front side of the body (1) is provided with a control panel (5), the top of the control panel (5) is provided with a display screen (4), and the bottom of the display screen (4) is provided with several control buttons (6).

7. The portable isothermal amplification detection device according to claim 1, characterized in that: The top of the body (1) is rotatably connected to a top cover (2), and a latch (3) is provided on the front side of the top cover (2). The body (1) and the top cover (2) are interlocked by the latch (3).

8. The portable isothermal amplification detection device according to claim 1, characterized in that: Anti-slip pads (9) are fixedly connected to the four corners of the bottom of the body (1).

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