Method for constructing DNA fingerprint of chrysosporium keratinophilum and identifying chrysosporium keratinophilum variety by applying combination of missense coding region MNP molecular markers

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By constructing a DNA fingerprint map of Auricularia auricula-judae based on missense region (MNP) molecular markers, the problem of poor universality in Auricularia auricula-judae variety identification was solved, enabling rapid and accurate variety identification and germplasm resource management, and promoting the breeding of new varieties.

CN121137220BActive Publication Date: 2026-06-23YUNNAN JUNSHIJIE BIOTECHNOLOGY CO LTD

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Patent Information

Authority / Receiving Office: CN · China
Patent Type: Patents(China)
Current Assignee / Owner
Filing Date: 2025-09-15
Publication Date: 2026-06-23

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Patent Timeline

15 Sep 2025

Application

23 Jun 2026

Publication

CN121137220B

IPC: C12Q1/6895; C12N15/11; G16B30/10; C12R1/645

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Microbiological testing/measurement Microorganism based processes

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BiotechnologyGenetic diversity

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Abstract

The application discloses a method for constructing a DNA fingerprint of Tremella aurantialba and identifying a Tremella aurantialba variety by using a MNP molecular marker combination located in a missense coding region, wherein the MNP molecular marker combination is composed of 108 MNP molecular markers, and the method for constructing the DNA fingerprint of the Tremella aurantialba by using the MNP molecular marker combination comprises the following steps: extracting DNA of Tremella aurantialba germplasm resources or varieties used for constructing the fingerprint, and detecting genotypes of 108 MNP sites; and then, arranging the genotypes of the 108 MNP sites of each Tremella aurantialba germplasm resource or variety according to a site number order to form a specific sequence, namely the DNA fingerprint of the Tremella aurantialba. Further, the DNA fingerprint of the Tremella aurantialba is used for identifying Tremella aurantialba varieties. The molecular marker combination has high specificity and resolution, and can reveal a real level of genetic diversity of the Tremella aurantialba. By using the MNP molecular marker combination to establish a DNA fingerprint library, Tremella aurantialba varieties can be identified, and the detection result is accurate and efficient.

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Description

Technical Field

[0001] This invention relates to the field of molecular biology technology of golden ear fungus, and in particular to a method for constructing a DNA fingerprint map of golden ear fungus using MNP molecular markers located in the missense coding region, and a method for identifying golden ear fungus varieties using the constructed map. Background Technology

[0002] Golden ear fungus is an edible and medicinal fungus. Its fruiting body is rich in polysaccharides, carotenoids, amino acids, minerals, and vitamins, and it has now been domesticated and industrialized. However, as more and more people cultivate golden ear fungus, the current identification of varieties mainly relies on ITS sequences and SSR markers, which suffers from poor universality, limited coverage, and a lack of standardized management. This leads to unclear understanding of varieties, often resulting in "synonyms," and also presents some problems in defining and protecting selected varieties. There is an urgent need to develop a rapid and convenient identification method.

[0003] DNA fingerprinting, based on molecular markers, offers significant advantages for identifying germplasm resources. Commonly used molecular markers include restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), simple repeat sequence (SSR), single nucleotide polymorphism (SNP), insertion / deletion (INDEL), and multinucleotide polymorphism (MNP). However, there are few reports on using MNP marker genotyping technology to construct fingerprint profiles for *Auricularia auricula-judae* (Golden Ear) and to identify its germplasm using MNP markers. Therefore, constructing a DNA fingerprint database of MNP sites located in missense coding regions is of great significance for efficient identification of *Auricularia auricula-judae* varieties and further breeding of new varieties. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a method for constructing a DNA fingerprint of *Auricularia auricula-judae* using a combination of molecular markers located in the missense coding region (MNP) and a method for identifying *Auricularia auricula-judae* varieties using the fingerprint.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] A method for constructing a DNA fingerprint of *Auricularia auricula-judae* using a combination of MNP molecular markers located in the missense coding region, wherein the MNP molecular marker combination consists of 108 MNP molecular markers, numbered Na-001 to Na-108, and the molecular marker information is as follows:

[0007]

[0008] In the above information, the markers are interpreted as follows: The molecular marker with MNP number Na-001 is located on chromosome 1, with physical positions from 157077 to 157177, containing 7 SNPs at physical positions 157077, 157090, 157091, 157092, 157099, 157100, and 157140. The alleles corresponding to these physical positions are T / C, A / G, A / G, T / C, T / C, T / C, and T / C, respectively. Among these, 157077, 157091, 157092, 157100, and 157140 are missense coding regions, encoding the gene NAU27000055. The interpretations of the other molecular markers are similar to those of the Na-001 molecular marker.

[0009] The method for constructing a DNA fingerprint of *Eurydon cylindrica* using the MNP molecular marker combination is as follows:

[0010] S1. Extract DNA from the golden ear germplasm resources or varieties used to construct fingerprint maps and detect the genotypes of 108 MNP loci from Na-001 to Na-108.

[0011] S2. For each Golden Ear germplasm resource or variety, the genotypes at the 108 MNP loci are combined and sorted according to the locus numbering order to form a specific sequence. This specific sequence is the Golden Ear DNA fingerprint map. The map number, name, and DNA fingerprint are as follows:

[0012] Serial Number name DNA fingerprinting 1 18A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 2 1A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C T / CC / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C T / T T / T C / C G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 3 27A C / C G / G G / G C / C C / C C / C C / C G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T G / G C / C C / C T / T C / CC / C A / A T / T A / A A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C T / T T / T C / C T / TC / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A A / A G / G C / C A / A G / G C / CG / G A / A T / T C / C T / T T / T G / G A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C T / T C / C A / A G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C A / A T / T G / G G / G G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T G / G C / C G / G T / T T / T A / A T / TT / T T / T A / A A / A C / C A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GA / A T / T G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CC / CC / CG / GT / TT / TA / AC / CA / AC / CT / TT / TC / CT / TG / GG / GA / AC / CA / AT / TT / TA / AA / AG / GG / GA / AC / CC / CA / AT / TT / TA / AA / AG / GA / AC / CT / TA / AC / CC / CT / TT / TC / CC / CG / GC / CC / CC / CT / TT / TC / CC / CC / CT / TG / GT / TG / GC / CG / GC / CA / AG / GG / GG / GC / CA / AC / CG / GC / C 4 29A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A T / T G / G G / G C / CG / G A / A C / C T / T C / C T / T C / C T / T T / T C / C G / G C / C C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A C / C T / T C / C T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C T / T T / T C / C C / CG / G G / G C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G C / C C / C A / A A / A T / T T / T G / G C / C T / T A / A G / G C / CG / G A / A T / T C / C T / T T / T G / G T / T G / G C / C G / G T / T C / C A / A C / CT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A G / G A / A T / T G / GA / A G / G C / C T / T A / A T / T A / A G / G C / C A / A G / G T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A C / CA / A A / A T / T G / G A / A C / C T / T A / A T / T A / A T / T A / A T / T G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T T / T T / T T / T G / GA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CC / CC / CG / GT / TT / TA / AC / GA / GC / CT / TT / TC / CT / TG / GG / GA / AC / CT / TA / AC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AA / AC / CT / TA / AC / CC / CA / AT / TC / CG / GA / AT / TT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AG / GT / TG / GA / AC / CA / AA / AA / AA / AA / AT / TA / AC / CG / GC / C 5 45A T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T A / A G / G G / G A / A G / G C / C T / T G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G G / G T / T A / A G / G G / G A / A T / T T / T G / GA / A A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C T / T T / T C / C C / CG / G G / G T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T G / G C / C T / T G / G A / A T / TG / C A / C T / A C / T T / C C / C T / T T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C A / A A / A C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G A / A A / A T / T C / C A / A G / G G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T C / C G / G G / G G / G G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AG / G G / G G / G G / G A / A A / A C / C C / C G / G T / T T / T G / G G / G T / T G / GG / G C / C G / G T / T A / A T / T G / G A / A G / G C / C G / G G / G C / C A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CC / CC / CA / AC / CC / CC / CC / CA / AC / CT / TG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / AC / AC / CT / TT / TT / TG / GC / CC / CG / GC / CA / AG / GT / TT / TA / AG / GT / TT / TT / AC / TG / GA / AT / TC / CC / CC / CT / TT / TC / CC / CC / CT / TG / GT / TG / GC / CG / GT / TG / GA / AA / AA / AT / TA / AC / CG / GC / C 6 58A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T G / G C / C C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A C / C T / T C / C T / T T / T C / CG / G G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T C / C C / C G / G T / TC / C T / T T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T T / T T / T G / G G / G C / C A / A A / A G / G C / C G / G A / A T / TG / G A / A T / A C / T T / T C / C T / T T / T G / G T / T T / T T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C T / T T / T G / G T / T T / T C / C G / G A / A A / A C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C T / T A / A T / T A / A A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TT / T T / T A / A G / G A / A G / G T / T C / C G / G T / T T / T G / G G / G T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A G / G C / C A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TA / AC / CC / CC / CC / GA / GC / TT / GT / TC / CT / TG / GG / GA / AC / CA / AT / TT / TA / AA / AG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AT / TG / GC / CG / GC / CA / AG / GG / GG / GC / CA / AC / CG / GC / C 7 5A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / C A / C T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 8 9A C / T G / A G / A C / T C / T C / T C / T G / C C / G A / C G / A T / C G / T G / A C / TG / A A / G C / T A / T G / C G / T A / C G / T C / T T / C G / A C / G C / A T / C C / AC / A A / G T / C A / C A / G C / G A / G G / T G / A C / G T / G C / A T / G T / C G / CA / G G / A C / T C / T T / C A / G C / T G / A T / C A / G C / T T / T T / T C / G T / CC / G T / G C / T C / G A / G C / A T / C A / C T / A A / T C / G G / A G / C C / T A / GC / T T / A G / T G / A T / G T / C C / G A / G A / C T / A T / C C / A T / C A / G T / CG / A C / G T / G C / T C / T A / G A / G C / T A / T A / G G / C C / T A / G G / A C / TG / G A / A T / A C / T T / C T / C G / T A / T A / G C / T G / T T / C C / T A / T C / TG / T C / G C / T C / T G / C C / T G / A G / A T / C C / G G / T A / G G / A T / G G / TC / A A / C G / T A / C C / T C / T G / A T / C T / C C / G G / C A / G A / C T / C A / TC / A A / G C / A G / A G / C G / A A / G C / T G / A G / C T / A G / A A / T T / C G / AA / C G / C C / T C / T G / A C / T G / A A / G A / C G / A C / G T / A G / A C / T C / TG / A T / G C / T T / C C / G A / G G / A G / A G / A G / A G / T T / C G / A A / G G / CT / A G / A G / T C / G G / A A / C A / T G / A C / T A / C T / G G / A G / T G / T A / GC / C G / A A / G C / G C / A T / C A / G T / C G / A C / T G / A T / A T / A A / C T / AT / G T / G A / G A / G C / A A / G C / T T / C A / G C / T C / T T / G T / G T / C G / AA / G T / C G / G T / T A / A T / T G / A G / A A / G T / C A / G A / G T / C A / CT / CT / C T / C A / G C / T T / C A / C C / T C / T C / G C / T C / T G / A T / C T / C A / CC / C A / A C / T T / G T / G C / T T / G G / A G / C A / C C / A A / T T / A T / C A / AA / A G / A G / C A / T C / T C / T A / G T / C T / C A / G A / C G / A A / G C / T T / GA / G C / G C / T T / A T / T C / C C / G G / A C / T C / T C / T C / G T / C T / C C / TC / A C / T T / C G / A T / G G / T C / G G / A C / T A / G G / A G / A G / A C / T A / GC / G G / A C / T 9 BY1A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 10 D2A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A T / T G / G G / G T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C A / A G / G C / C T / T C / CC / C A / A T / T A / A G / G G / G A / A G / G G / G G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C G / G C / C A / A C / C A / A T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A A / A G / G C / C G / G A / A T / TG / G A / A T / T C / C T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C T / T C / C A / A G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T A / A T / T G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A G / G T / T T / T A / A C / C C / C T / T T / T C / C A / AG / G C / C A / A G / G G / G C / C A / A G / G A / A T / T A / A A / A T / T C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T G / G T / T T / T A / AC / C A / A C / C T / T T / T C / C T / T G / G G / G A / A C / C T / T A / A C / C G / GC / C G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T G / GG / G G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C G / GG / G A / A T / T 11 D3A T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T C / C C / C G / G T / TC / C T / T T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G T / T C / C A / A A / A T / T C / C A / A T / T A / A T / TG / G C / C T / T T / T T / T G / G G / G C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / T C / C T / C T / T G / G T / T G / G C / C G / G C / C T / T T / T T / TG / G C / C C / C C / C G / G C / C A / A A / A C / C C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T G / G T / T T / T C / C G / G A / A A / A C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G A / A A / A G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C A / A T / T A / A T / T G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T T / T A / A T / T T / T G / G G / G T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TA / AC / CC / CC / CC / CA / AC / CT / TG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / AC / AC / CT / TT / TT / TG / GC / CC / CG / GC / CA / AG / GT / TT / TA / AG / GC / CT / AT / TC / CG / GA / AT / TC / CC / CC / CT / TT / TC / CC / CC / CT / TG / GT / TG / GG / GA / AT / TG / GA / AA / AA / AT / TA / AC / CG / GC / C 12 D51A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A T / T C / C T / C T / T G / G A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A A / A G / G A / A A / A T / T T / TA / A G / G T / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C A / A C / C T / T A / A C / C C / C T / T T / T C / C A / AA / A T / T G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TG / GT / TT / TA / AC / CA / AC / TT / GG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TC / CC / CT / TG / GT / TG / GG / GA / AC / CA / AA / AA / AA / AT / TA / AC / CG / GC / C 13 D60A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / GA / A G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T T / T T / T C / C T / TC / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T G / G C / C T / T A / A G / G C / CG / G A / A T / T C / C T / C C / C T / T T / T G / G T / T T / T T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C A / A T / T A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T G / G C / C G / G T / T A / A C / C A / AG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T C / C A / AG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TG / GT / TT / TA / AC / CA / AC / CT / TT / TC / CT / TG / GG / GA / AC / CA / AT / TC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AG / GT / TG / GA / AC / CA / AG / GG / GG / GC / CG / GG / GA / AT / T 14 D9A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G G / G T / T T / T C / C G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G C / C C / C A / A A / A C / C A / A A / A G / G C / C G / G A / A T / TG / G A / A T / A C / T T / T T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C T / T A / A T / T A / A G / G C / C G / G C / C T / T G / G T / T T / TA / A G / G T / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C C / C G / G G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G A / A C / C G / G T / T T / T A / A C / C C / C T / T T / T T / T G / GA / A T / T A / A G / G G / G C / C A / A G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TA / AC / CC / CC / CC / CA / AC / CT / TT / TC / CT / TG / GG / GA / AC / CA / AT / TT / TA / AA / AG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / TT / AC / TC / CG / GC / CT / TT / TG / GC / CC / CT / TC / CC / CT / TG / GT / TG / GG / GA / AC / CA / AG / GG / GG / GC / CA / AC / CG / GC / C 15 F1_2-9 C / T G / A G / A C / T C / T C / T C / T G / C C / G A / C G / A T / C G / T G / A C / TG / A A / G C / T A / T G / C G / T A / C G / T C / T T / C G / A C / G C / A T / C C / AC / A A / G T / C A / C A / G C / G A / G G / T G / A C / G T / G C / A T / G T / C G / CA / G G / A C / T C / T T / C A / G C / T G / A T / C A / G C / T T / C T / C C / G T / CC / G T / G C / T C / G A / G C / A T / C A / C T / A A / T C / G G / A G / C C / T A / GC / T T / A G / T G / A T / G T / C C / G A / G A / C T / A T / C C / A T / C A / G T / CG / A C / G T / G C / T C / T A / G A / G C / T A / T A / G G / C C / T A / G G / A C / TG / G A / A T / A C / T T / C T / C G / T A / T A / G C / T G / T T / C C / T A / T C / TG / T C / G C / T C / T G / C C / T G / A G / A T / C C / G G / T A / G G / A T / G G / TC / A A / C G / T A / C C / T C / T G / A T / C T / C C / G G / C A / G A / C T / C A / TC / A A / G C / A G / A G / C G / A A / G C / T G / A G / C T / A G / A A / T T / C G / AA / C G / C C / T C / T G / A C / T G / A A / G A / C G / A C / G T / A G / A C / T C / TG / A T / G C / T T / C C / G A / G G / A G / A G / G G / G G / T T / C G / A A / G G / CT / A G / A G / T C / G G / A A / C A / T A / A T / T A / A T / T G / A G / T G / T A / GC / G G / A A / G C / G C / A T / C A / G T / C G / A C / T G / A T / A A / A C / C T / AT / G T / G A / G A / A C / C A / G C / T T / C A / G C / T C / T T / G T / G T / C G / AA / G T / C G / A T / G A / G T / C G / A G / A A / G T / C A / G A / G T / C A / CT / CT / C T / C A / G C / T T / C A / C C / T C / T C / G C / C C / C G / A T / C T / C A / CC / C A / A C / C T / T T / G C / T T / G G / A G / C A / A C / C A / T T / A T / C A / GA / C A / A C / C T / T T / T T / T G / G C / C T / C A / G A / C G / A A / G C / T T / GA / G C / G C / T T / A T / T C / C C / G G / A C / T C / T C / T C / G T / C T / C C / TC / A C / T T / C G / A T / G G / T C / G G / A C / T A / G G / A G / A G / A C / T A / GC / G G / A C / T 16 F1-21A C / T G / A G / A C / T C / T C / T C / T G / C C / G A / C G / A C / C T / T A / A T / TA / A G / G T / T T / T C / C G / T A / C G / T C / T T / C G / A C / G A / A C / C A / AA / A G / G C / C C / C A / G C / G A / G G / T G / A C / G T / G C / A T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / C T / C C / G T / CC / G T / G C / T C / G A / G C / A T / C A / C T / A A / T C / G G / A G / C C / T A / GC / T T / T G / G G / A T / G C / C G / G A / G A / C T / A T / C C / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G C / T A / T A / G G / C C / T A / G G / A C / TG / G A / A T / T C / C T / T C / C T / T A / A A / A C / C G / G T / C C / T A / T C / TG / G C / C C / C C / C G / G C / C G / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / C A / TC / A A / G C / A G / A G / C G / A A / G C / T G / A G / C T / A G / A A / T T / C G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / T C / TG / A T / G C / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / CT / A G / A G / T C / G G / A A / C A / T G / A C / T A / A T / T G / A G / G G / T A / GC / G A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / T T / C A / C C / T C / T C / G C / T C / T G / G T / T T / T A / AC / C A / A C / T T / G T / G C / T T / G G / A G / C A / C C / A A / A T / A T / T A / AA / A G / A G / C A / T C / T C / T A / G T / C T / T A / A A / C G / A A / G C / T T / GA / G C / C C / C A / A T / T C / C G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G C / T A / G G / A G / A G / A T / T A / GC / G G / A C / T 17 F2A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 18 K18-21A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A T / T C / C T / T C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / C T / T T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 19 KF1_23 T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / GA / A A / A T / T T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C T / TC / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T G / G A / A T / TG / G A / A T / T C / C T / C C / C T / T T / T G / G T / T T / T T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C T / T T / T G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G T / T T / TA / A G / G T / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C A / A T / T G / G G / G G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C A / A C / C C / C G / G C / C C / C T / T T / T C / C A / AA / A T / T G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G G / G T / T G / G A / A C / C C / C A / A A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A G / G T / T T / A T / T C / C C / C G / G C / C C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A G / G C / C G / GG / G A / A T / T 20 LLA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / T T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 21 MD T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C A / A C / C C / C G / G C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TG / GT / TT / TA / AC / GA / GC / CT / TG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AA / AG / GA / AC / CT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AG / GT / TG / GA / AT / TG / GG / GG / GA / AT / TG / GG / GA / AT / T 22 MX9A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 C / C G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 23 NLA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C T / C T / C C / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / G C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 24 X18A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / C A / C T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / G A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 25 XDA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 26 XR11A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / C T / T T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 27 YLA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 28 YRA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C

[0013] The method for identifying *Auricularia auricula-judae* varieties using the DNA fingerprinting constructed in this invention involves extracting DNA from the spores of the *Auricularia auricula-judae* germplasm resources or varieties to be tested, detecting the genotypes of 108 MNP loci (Na-001~Na-108) in the genomic DNA of the germplasm resources, determining the genotype of the MNP molecular markers based on the MNP loci detection results, comparing it with the constructed DNA fingerprinting, and calculating the genetic similarity GS. When the genetic similarity GS of the *Auricularia auricula-judae* germplasm resources or varieties to be tested is greater than or equal to 96%, they are identified as the same germplasm or variety; the genetic similarity GS = n / N × 100%, where N is the number of MNP loci, and n is the number of MNP loci with the same genotype for each *Auricularia auricula-judae* germplasm resource or variety.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] This invention relates to a molecular marker ensemble for constructing DNA fingerprinting of *Auricularia auricula-judae* (golden ear fungus). Composed of 108 missense region (MNP) molecular markers (333 SNPs), it possesses high specificity and resolution, enabling the accurate utilization of the true level of genetic diversity in *Auricularia auricula-judae*. The DNA fingerprinting library established using this MNP molecular marker ensemble accurately reflects the DNA fingerprinting information of different *Auricularia auricula-judae* varieties, facilitating variety identification. The detection results are precise and efficient, effectively distinguishing 28 *Auricularia auricula-judae* germplasm resource varieties, providing excellent technical support for the protection of *Auricularia auricula-judae* germplasm resources. Attached Figure Description

[0016] Figure 1 This invention presents a phylogenetic tree of 28 golden ear germplasm resources constructed based on 108 missense coded MNPs (333 SNPs);

[0017] Figure 2 This shows the genotyping results of 28 *Auricularia auricula-judae* materials in Example 2 of this invention. Each row represents one material, and each column represents one SNP genotype. Blue, red, purple, and orange represent alleles C / C, A / A, G / G, and T / T, respectively. Unknown alleles are shown in white, and alleles are represented by 0 / 0; heterozygous sites are shown in gray.

[0018] Figure 3 for Figure 2 Enlarged view of segment A in the image;

[0019] Figure 4 for Figure 2 Enlarged view of segment B in the image;

[0020] Figure 5 for Figure 2 Enlarged view of segment C in the image;

[0021] Figure 6 for Figure 2Enlarged view of segment D in the image;

[0022] Figure 7 This shows the genetic similarity results of 28 golden ear fungus materials in Example 2 of this invention. The genetic similarity changes from blue to red.

[0023] Figure 8 This shows the genotyping results of the golden ear fungus materials D13A and 3-13, D25A and 3-25, D40A and 3-40, and D47A and 3-47 in Example 3 of this invention. Each row represents one material, and each column represents one SNP genotype. Blue, red, purple, and orange represent alleles C / C, A / A, G / G, and T / T, respectively. Unknown alleles are white, and alleles are represented by 0 / 0; heterozygous sites are represented by gray.

[0024] Figure 9 for Figure 8 Enlarged view of segment A in the image;

[0025] Figure 10 for Figure 8 Enlarged view of segment B in the image;

[0026] Figure 11 for Figure 8 Enlarged view of segment C in the image;

[0027] Figure 12 for Figure 8 Enlarged view of segment D in the image;

[0028] Figure 13 The genetic similarity results of D13A and 3-13, D25A and 3-25, D40A and 3-40, and D47A and 3-47 golden ear materials in Example 3 of the present invention are shown, where the genetic similarity gradually changes from blue to red. Detailed Implementation

[0029] The specific embodiments of the present invention will be described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0030] Example 1. Missense coding region (MNP) sites (molecular marker combinatorial) used to construct a DNA fingerprint of *Eurydon edulis*.

[0031] This invention uses 28 Auricularia auricula germplasm resource samples, the sample information of which is shown in Table 1. Genomic DNA was extracted from Auricularia auricula buds and whole-genome resequencing was completed at BGI Genomics Co., Ltd. in Shenzhen. Based on the sequencing data, 108 missense coding region MNPs (333 SNPs) were obtained.

[0032] Table 1. Information on 28 Golden Ear Germplasm Resource Samples

[0033] Serial Number strain strain type source 1 18A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 2 1A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 3 27A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 4 29A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 5 45A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 6 58A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 7 5A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 8 9A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 9 BY1A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 10 D2A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 11 D3A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 12 D51A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 13 D60A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 14 D9A Single spore Yunnan Junshijie Biotechnology Co., Ltd. 15 F1_2-9 Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 16 F1-21A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 17 F2A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 18 K18-21A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 19 KF1_23 Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 20 LLA Cultivated varieties Gutian area, Fujian 21 MD Single spore Yunnan Junshijie Biotechnology Co., Ltd. 22 MX9A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 23 NLA Cultivated varieties Ninglang area of Lijiang, Yunnan 24 X18A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 25 XDA Cultivated varieties Xundian area of Kunming, Yunnan 26 XR11A Cultivated varieties Yunnan Junshijie Biotechnology Co., Ltd. 27 YLA Cultivated varieties Yunlong area of Dali, Yunnan 28 YRA Cultivated varieties Yongren area of Chuxiong, Yunnan

[0034] The MNP site screening method is as follows:

[0035] (1) Trimmomatic-0.39 was used for quality control of sequencing data, with default parameters;

[0036] (2) The quality-controlled data were compared with the reference genome Ns_27 (NCBI project number: PRJNA1264744) using BWA 0.7.17 software to generate a sam file;

[0037] (3) Use SAMtools 1.22 to convert the SAM file to a BAM file, and then sort and remove duplicates. Analyze each material separately to ensure the accuracy of individual variation;

[0038] (4) Use GATK 4.5 to merge the bam files of each strain, and then perform mutation detection to obtain SNP data;

[0039] (5) The SNP data were further screened using VCFtools 0.1.17, with the criteria being a deletion rate of more than 20% and a minimum allele frequency of less than 0.05, resulting in 131,632 SNPs.

[0040] (6) Using a sliding window length of 100 bp, the 131,632 obtained SNPs were used to detect windows containing 2 to 10 SNPs. Intervals with a polymorphism information content (PIC) less than 0.4 and overlapping were removed. Finally, 343 MNPs (1120 SNPs) were selected based on a minimum adjacent window spacing of 50 kb.

[0041] (7) SnpEff 5.2 was used to annotate the 343 MNPs (1120 SNPs) obtained. Sites located in the coding region and which are missense variants were screened, and finally 108 MNPs (333 SNPs) were obtained. See Table 2 for details.

[0042] Table 2. Molecular marker information of 108 missense coding regions (MNPs) and 333 SNPs in *Eurydon cylindrica*

[0043]

[0044] In Table 1, the meaning of the markers is as follows: The molecular marker with MNP number Na-001 is located on chromosome 1, with physical positions from the beginning to the end of 157077-157177, containing 7 SNPs with physical positions of 157077, 157090, 157091, 157092, 157099, 157100, and 157140, respectively. The alleles corresponding to the above physical positions are T / C, A / G, A / G, T / C, T / C, T / C, and T / C, respectively. Among them, 157077, 157091, 157092, 157100, and 157140 are missense coding regions, and the coding gene is NAU27000055. The meaning of other molecular markers is explained in the same way as that of the molecular marker with the number Na-001.

[0045] Example 2. Genotyping and fingerprinting of *Auricularia auricula-judae* germplasm resources.

[0046] Constructing a DNA fingerprint: DNA was extracted from spore samples of *Auricularia auricula-judae* germplasm resources used for constructing the DNA fingerprint. Genotypes at 108 MNPs (333 SNPs) in the *Auricularia auricula-judae* germplasm samples were obtained through whole-genome resequencing at BGI Genomics Co., Ltd. in Shenzhen. Based on the genotyping results, the genotypes at the 108 MNPs (333 SNPs) for each *Auricularia auricula-judae* germplasm resource were combined and sorted according to the locus numbering order to form a specific sequence. This specific sequence constitutes the DNA fingerprint of the *Auricularia auricula-judae* germplasm resource or variety, as detailed in Table 3. A phylogenetic tree was constructed using the 108 MNPs (333 SNPs) located in the coding region and exhibiting missense variants. These 108 MNPs (333 SNPs) reflect the true population diversity, as detailed in Table 3. Figure 1 The phylogenetic tree shown is based on 108 missense MNPs (333 SNPs) constructed according to this invention for 28 *Auricularia auricula-judae* germplasm resources. Genotyping results of the 28 *Auricularia auricula-judae* materials are shown below. Figure 2 Each row represents one data set, and each column represents a SNP genotype. Blue, red, purple, orange, and white represent alleles C / C, A / A, G / G, T / T, and 0 / 0, respectively. Heterozygous sites are shown in gray. For clearer display... Figure 2 ,Will Figure 2 Segmented magnification, divided into Figure 3 , Figure 4 , Figure 5 and Figure 6 The genetic similarity results of 28 golden ear materials are shown in the figure. Figure 7 The genetic similarity gradient changes from blue to red. Among the 28 *Auricularia auricula-judae* materials, cultivated strains 18A, 5A, LLA, NLA, X18A, XDA, YLA, and YRA all showed similarities exceeding 96%, suggesting they were likely obtained asexually from the same strain. The same was true for cultivated strains 1A, BY1A, F2A, K18-21A, MX9A, and XR11A. Table 3 shows the fingerprint patterns of *Auricularia auricula-judae* germplasm resources.

[0047] Serial Number name DNA fingerprinting 1 18A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 2 1A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C T / CC / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C T / T T / T C / C G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 3 27A C / C G / G G / G C / C C / C C / C C / C G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T G / G C / C C / C T / T C / CC / C A / A T / T A / A A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C T / T T / T C / C T / TC / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A A / A G / G C / C A / A G / G C / CG / G A / A T / T C / C T / T T / T G / G A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C T / T C / C A / A G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C A / A T / T G / G G / G G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T G / G C / C G / G T / T T / T A / A T / TT / T T / T A / A A / A C / C A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GA / A T / T G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CC / CC / CG / GT / TT / TA / AC / CA / AC / CT / TT / TC / CT / TG / GG / GA / AC / CA / AT / TT / TA / AA / AG / GG / GA / AC / CC / CA / AT / TT / TA / AA / AG / GA / AC / CT / TA / AC / CC / CT / TT / TC / CC / CG / GC / CC / CC / CT / TT / TC / CC / CC / CT / TG / GT / TG / GC / CG / GC / CA / AG / GG / GG / GC / CA / AC / CG / GC / C 4 29A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A T / T G / G G / G C / CG / G A / A C / C T / T C / C T / T C / C T / T T / T C / C G / G C / C C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A C / C T / T C / C T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C T / T T / T C / C C / CG / G G / G C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G C / C C / C A / A A / A T / T T / T G / G C / C T / T A / A G / G C / CG / G A / A T / T C / C T / T T / T G / G T / T G / G C / C G / G T / T C / C A / A C / CT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A G / G A / A T / T G / GA / A G / G C / C T / T A / A T / T A / A G / G C / C A / A G / G T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A C / CA / A A / A T / T G / G A / A C / C T / T A / A T / T A / A T / T A / A T / T G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T T / T T / T T / T G / GA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CC / CC / CG / GT / TT / TA / AC / GA / GC / CT / TT / TC / CT / TG / GG / GA / AC / CT / TA / AC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AA / AC / CT / TA / AC / CC / CA / AT / TC / CG / GA / AT / TT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AG / GT / TG / GA / AC / CA / AA / AA / AA / AA / AT / TA / AC / CG / GC / C 5 45A T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T A / A G / G G / G A / A G / G C / C T / T G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G G / G T / T A / A G / G G / G A / A T / T T / T G / GA / A A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C T / T T / T C / C C / CG / G G / G T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T G / G C / C T / T G / G A / A T / TG / C A / C T / A C / T T / C C / C T / T T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C A / A A / A C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G A / A A / A T / T C / C A / A G / G G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T C / C G / G G / G G / G G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AG / G G / G G / G G / G A / A A / A C / C C / C G / G T / T T / T G / G G / G T / T G / GG / G C / C G / G T / T A / A T / T G / G A / A G / G C / C G / G G / G C / C A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CC / CC / CA / AC / CC / CC / CC / CA / AC / CT / TG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / AC / AC / CT / TT / TT / TG / GC / CC / CG / GC / CA / AG / GT / TT / TA / AG / GT / TT / TT / AC / TG / GA / AT / TC / CC / CC / CT / TT / TC / CC / CC / CT / TG / GT / TG / GC / CG / GT / TG / GA / AA / AA / AT / TA / AC / CG / GC / C 6 58A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T G / G C / C C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A C / C T / T C / C T / T T / T C / CG / G G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T C / C C / C G / G T / TC / C T / T T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T T / T T / T G / G G / G C / C A / A A / A G / G C / C G / G A / A T / TG / G A / A T / A C / T T / T C / C T / T T / T G / G T / T T / T T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C T / T T / T G / G T / T T / T C / C G / G A / A A / A C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C T / T A / A T / T A / A A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TT / T T / T A / A G / G A / A G / G T / T C / C G / G T / T T / T G / G G / G T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A G / G C / C A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TA / AC / CC / CC / CC / GA / GC / TT / GT / TC / CT / TG / GG / GA / AC / CA / AT / TT / TA / AA / AG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AT / TG / GC / CG / GC / CA / AG / GG / GG / GC / CA / AC / CG / GC / C 7 5A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / C A / C T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 8 9A C / T G / A G / A C / T C / T C / T C / T G / C C / G A / C G / A T / C G / T G / A C / TG / A A / G C / T A / T G / C G / T A / C G / T C / T T / C G / A C / G C / A T / C C / AC / A A / G T / C A / C A / G C / G A / G G / T G / A C / G T / G C / A T / G T / C G / CA / G G / A C / T C / T T / C A / G C / T G / A T / C A / G C / T T / T T / T C / G T / CC / G T / G C / T C / G A / G C / A T / C A / C T / A A / T C / G G / A G / C C / T A / GC / T T / A G / T G / A T / G T / C C / G A / G A / C T / A T / C C / A T / C A / G T / CG / A C / G T / G C / T C / T A / G A / G C / T A / T A / G G / C C / T A / G G / A C / TG / G A / A T / A C / T T / C T / C G / T A / T A / G C / T G / T T / C C / T A / T C / TG / T C / G C / T C / T G / C C / T G / A G / A T / C C / G G / T A / G G / A T / G G / TC / A A / C G / T A / C C / T C / T G / A T / C T / C C / G G / C A / G A / C T / C A / TC / A A / G C / A G / A G / C G / A A / G C / T G / A G / C T / A G / A A / T T / C G / AA / C G / C C / T C / T G / A C / T G / A A / G A / C G / A C / G T / A G / A C / T C / TG / A T / G C / T T / C C / G A / G G / A G / A G / A G / A G / T T / C G / A A / G G / CT / A G / A G / T C / G G / A A / C A / T G / A C / T A / C T / G G / A G / T G / T A / GC / C G / A A / G C / G C / A T / C A / G T / C G / A C / T G / A T / A T / A A / C T / AT / G T / G A / G A / G C / A A / G C / T T / C A / G C / T C / T T / G T / G T / C G / AA / G T / C G / G T / T A / A T / T G / A G / A A / G T / C A / G A / G T / C A / CT / CT / C T / C A / G C / T T / C A / C C / T C / T C / G C / T C / T G / A T / C T / C A / CC / C A / A C / T T / G T / G C / T T / G G / A G / C A / C C / A A / T T / A T / C A / AA / A G / A G / C A / T C / T C / T A / G T / C T / C A / G A / C G / A A / G C / T T / GA / G C / G C / T T / A T / T C / C C / G G / A C / T C / T C / T C / G T / C T / C C / TC / A C / T T / C G / A T / G G / T C / G G / A C / T A / G G / A G / A G / A C / T A / GC / G G / A C / T 9 BY1A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 10 D2A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A T / T G / G G / G T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C A / A G / G C / C T / T C / CC / C A / A T / T A / A G / G G / G A / A G / G G / G G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C G / G C / C A / A C / C A / A T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A A / A G / G C / C G / G A / A T / TG / G A / A T / T C / C T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C T / T C / C A / A G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T A / A T / T G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A G / G T / T T / T A / A C / C C / C T / T T / T C / C A / AG / G C / C A / A G / G G / G C / C A / A G / G A / A T / T A / A A / A T / T C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T G / G T / T T / T A / AC / C A / A C / C T / T T / T C / C T / T G / G G / G A / A C / C T / T A / A C / C G / GC / C G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T G / GG / G G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C G / GG / G A / A T / T 11 D3A T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T C / C C / C G / G T / TC / C T / T T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G T / T C / C A / A A / A T / T C / C A / A T / T A / A T / TG / G C / C T / T T / T T / T G / G G / G C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / T C / C T / C T / T G / G T / T G / G C / C G / G C / C T / T T / T T / TG / G C / C C / C C / C G / G C / C A / A A / A C / C C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T G / G T / T T / T C / C G / G A / A A / A C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G A / A A / A G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C A / A T / T A / A T / T G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T T / T A / A T / T T / T G / G G / G T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TA / AC / CC / CC / CC / CA / AC / CT / TG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / AC / AC / CT / TT / TT / TG / GC / CC / CG / GC / CA / AG / GT / TT / TA / AG / GC / CT / AT / TC / CG / GA / AT / TC / CC / CC / CT / TT / TC / CC / CC / CT / TG / GT / TG / GG / GA / AT / TG / GA / AA / AA / AT / TA / AC / CG / GC / C 12 D51A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A T / T C / C T / C T / T G / G A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A A / A G / G A / A A / A T / T T / TA / A G / G T / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C A / A C / C T / T A / A C / C C / C T / T T / T C / C A / AA / A T / T G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TG / GT / TT / TA / AC / CA / AC / TT / GG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TC / CC / CT / TG / GT / TG / GG / GA / AC / CA / AA / AA / AA / AT / TA / AC / CG / GC / C 13 D60A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / GA / A G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T T / T T / T C / C T / TC / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T G / G C / C T / T A / A G / G C / CG / G A / A T / T C / C T / C C / C T / T T / T G / G T / T T / T T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C A / A T / T A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T G / G C / C G / G T / T A / A C / C A / AG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T C / C A / AG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TG / GT / TT / TA / AC / CA / AC / CT / TT / TC / CT / TG / GG / GA / AC / CA / AT / TC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AG / GT / TG / GA / AC / CA / AG / GG / GG / GC / CG / GG / GA / AT / T 14 D9A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G G / G T / T T / T C / C G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G C / C C / C A / A A / A C / C A / A A / A G / G C / C G / G A / A T / TG / G A / A T / A C / T T / T T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C T / T A / A T / T A / A G / G C / C G / G C / C T / T G / G T / T T / TA / A G / G T / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C C / C G / G G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G A / A C / C G / G T / T T / T A / A C / C C / C T / T T / T T / T G / GA / A T / T A / A G / G G / G C / C A / A G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TA / AC / CC / CC / CC / CA / AC / CT / TT / TC / CT / TG / GG / GA / AC / CA / AT / TT / TA / AA / AG / GG / GA / AC / CC / CA / AT / TT / TA / AC / CA / AG / GT / TT / TA / AC / CC / CT / TT / AC / TC / CG / GC / CT / TT / TG / GC / CC / CT / TC / CC / CT / TG / GT / TG / GG / GA / AC / CA / AG / GG / GG / GC / CA / AC / CG / GC / C 15 F1_2-9 C / T G / A G / A C / T C / T C / T C / T G / C C / G A / C G / A T / C G / T G / A C / TG / A A / G C / T A / T G / C G / T A / C G / T C / T T / C G / A C / G C / A T / C C / AC / A A / G T / C A / C A / G C / G A / G G / T G / A C / G T / G C / A T / G T / C G / CA / G G / A C / T C / T T / C A / G C / T G / A T / C A / G C / T T / C T / C C / G T / CC / G T / G C / T C / G A / G C / A T / C A / C T / A A / T C / G G / A G / C C / T A / GC / T T / A G / T G / A T / G T / C C / G A / G A / C T / A T / C C / A T / C A / G T / CG / A C / G T / G C / T C / T A / G A / G C / T A / T A / G G / C C / T A / G G / A C / TG / G A / A T / A C / T T / C T / C G / T A / T A / G C / T G / T T / C C / T A / T C / TG / T C / G C / T C / T G / C C / T G / A G / A T / C C / G G / T A / G G / A T / G G / TC / A A / C G / T A / C C / T C / T G / A T / C T / C C / G G / C A / G A / C T / C A / TC / A A / G C / A G / A G / C G / A A / G C / T G / A G / C T / A G / A A / T T / C G / AA / C G / C C / T C / T G / A C / T G / A A / G A / C G / A C / G T / A G / A C / T C / TG / A T / G C / T T / C C / G A / G G / A G / A G / G G / G G / T T / C G / A A / G G / CT / A G / A G / T C / G G / A A / C A / T A / A T / T A / A T / T G / A G / T G / T A / GC / G G / A A / G C / G C / A T / C A / G T / C G / A C / T G / A T / A A / A C / C T / AT / G T / G A / G A / A C / C A / G C / T T / C A / G C / T C / T T / G T / G T / C G / AA / G T / C G / A T / G A / G T / C G / A G / A A / G T / C A / G A / G T / C A / CT / CT / C T / C A / G C / T T / C A / C C / T C / T C / G C / C C / C G / A T / C T / C A / CC / C A / A C / C T / T T / G C / T T / G G / A G / C A / A C / C A / T T / A T / C A / GA / C A / A C / C T / T T / T T / T G / G C / C T / C A / G A / C G / A A / G C / T T / GA / G C / G C / T T / A T / T C / C C / G G / A C / T C / T C / T C / G T / C T / C C / TC / A C / T T / C G / A T / G G / T C / G G / A C / T A / G G / A G / A G / A C / T A / GC / G G / A C / T 16 F1-21A C / T G / A G / A C / T C / T C / T C / T G / C C / G A / C G / A C / C T / T A / A T / TA / A G / G T / T T / T C / C G / T A / C G / T C / T T / C G / A C / G A / A C / C A / AA / A G / G C / C C / C A / G C / G A / G G / T G / A C / G T / G C / A T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / C T / C C / G T / CC / G T / G C / T C / G A / G C / A T / C A / C T / A A / T C / G G / A G / C C / T A / GC / T T / T G / G G / A T / G C / C G / G A / G A / C T / A T / C C / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G C / T A / T A / G G / C C / T A / G G / A C / TG / G A / A T / T C / C T / T C / C T / T A / A A / A C / C G / G T / C C / T A / T C / TG / G C / C C / C C / C G / G C / C G / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / C A / TC / A A / G C / A G / A G / C G / A A / G C / T G / A G / C T / A G / A A / T T / C G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / T C / TG / A T / G C / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / CT / A G / A G / T C / G G / A A / C A / T G / A C / T A / A T / T G / A G / G G / T A / GC / G A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / T T / C A / C C / T C / T C / G C / T C / T G / G T / T T / T A / AC / C A / A C / T T / G T / G C / T T / G G / A G / C A / C C / A A / A T / A T / T A / AA / A G / A G / C A / T C / T C / T A / G T / C T / T A / A A / C G / A A / G C / T T / GA / G C / C C / C A / A T / T C / C G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G C / T A / G G / A G / A G / A T / T A / GC / G G / A C / T 17 F2A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 18 K18-21A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A T / T C / C T / T C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / C T / T T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 19 KF1_23 T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / GA / A A / A T / T T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C T / TC / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T G / G A / A T / TG / G A / A T / T C / C T / C C / C T / T T / T G / G T / T T / T T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C T / T T / T G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G C / C G / G G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G T / T T / TA / A G / G T / T C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C A / A T / T G / G G / G G / G A / AC / C G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C A / A C / C C / C G / G C / C C / C T / T T / T C / C A / AA / A T / T G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G G / G T / T G / G A / A C / C C / C A / A A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A G / G T / T T / A T / T C / C C / C G / G C / C C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A G / G C / C G / GG / G A / A T / T 20 LLA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / T T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 21 MD T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G G / GT / T G / G G / G C / C G / G A / A A / A G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C A / A C / C C / C G / G C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / TT / TA / AC / CT / TA / AC / CC / CC / CT / TT / TG / GT / TT / TA / AC / GA / GC / CT / TG / GT / TG / GA / AC / CC / CA / AT / TA / AC / CG / GC / CG / GG / GA / AC / CC / CA / AT / TT / TA / AA / AG / GA / AC / CT / TA / AC / CC / CT / AT / TC / CC / CG / GC / CT / TT / TG / GC / CC / CT / TA / AT / TC / CA / AG / GT / TG / GA / AT / TG / GG / GG / GA / AT / TG / GG / GA / AT / T 22 MX9A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / T T / G T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 C / C G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 23 NLA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C T / C T / C C / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / G C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 24 X18A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / C A / C T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / G A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 25 XDA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 26 XR11A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / TA / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / C A / AA / A G / G C / C C / C G / G G / G A / A G / G G / G C / C T / T C / C T / T T / T G / GA / A G / G C / C T / T C / C G / G T / T A / A C / C G / G T / T T / T T / T C / C C / CG / G G / G T / T G / G G / G C / C T / T A / A T / T A / A C / C G / G G / G C / C A / AC / C T / T G / G A / A G / G C / C G / G G / G C / C A / A C / C A / A C / C G / G C / CA / A G / G G / G T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / TG / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A C / C G / G T / T C / C A / A C / CG / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / TA / A C / C T / T C / C C / C C / C G / G T / T T / T C / C G / G A / A A / A T / T A / AA / A G / G A / A A / A C / C A / A G / G T / T A / A G / G T / T G / G A / A T / T G / GA / A G / G C / C C / C G / G C / C G / G A / A A / A G / G C / C T / T G / G C / C C / CG / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / GT / T G / G G / G C / C G / G A / A A / A A / A T / T A / A T / T G / G G / G G / G A / AC / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / TG / G G / G G / G G / G A / A A / A C / C T / T A / A C / C C / C T / T T / T T / T G / GG / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / A T / T A / AT / TT / T T / T A / A C / C T / T A / A C / C C / C C / C C / C C / C G / G T / T T / T A / AC / C A / A C / C T / T T / T C / C T / T G / G G / G A / A C / C A / A T / T T / T A / AA / A G / G G / G A / A C / C C / C A / A T / T T / T A / A C / C A / A G / G T / T T / TA / A C / C C / C 0 / 0 0 / 0 0 / 0 G / G A / A T / T C / C C / C C / C T / T T / T C / CC / C C / C T / T G / G T / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / GG / G A / A T / T 27 YLA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C 28 YRA T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G T / T G / G G / G C / CG / G A / A C / C A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / T C / CC / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C C / CG / G A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / C G / G T / TC / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / GT / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / C T / T A / A T / TG / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T A / A G / G C / CG / G A / A T / A C / T T / C T / T G / G T / T G / G T / T T / T C / C T / T T / T T / TT / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / GC / C A / A G / G A / A T / T T / T A / A C / C C / C G / G C / C G / G C / C C / C T / TC / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / AC / C C / C T / T T / T A / A T / T A / A G / G C / C A / A G / G A / A A / A T / T T / TA / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / CA / A A / A T / T G / G A / A C / C T / T G / G C / C C / C G / G A / A T / T T / T G / GG / G G / G A / A C / C C / C T / T A / A T / T A / A T / T A / A A / A A / A C / C A / AT / T T / T A / A A / A C / C G / G T / T C / C G / G T / T T / T G / G G / G C / C A / AA / A T / T A / A G / G G / G C / C A / A A / A G / G C / C G / G G / G C / C C / CC / CC / C C / C G / G T / T C / C C / C T / T T / T G / G T / T T / T A / A C / C C / C C / CC / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / GC / C A / A C / C T / T T / T T / T G / G C / C C / C G / G A / A G / G A / A C / C G / GG / G G / G T / T T / A T / A C / T C / C G / G C / C T / T T / T G / G C / C C / C T / TA / A T / T C / C A / A G / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / AC / C G / G C / C

[0048] Example 3. Identification and genetic similarity calculation of golden ear germplasm resources.

[0049] A method for identifying a golden ear fungus variety includes extracting DNA from the golden ear fungus germplasm to be tested, using whole-genome resequencing to detect the genotypes of 108 MNP (333 SNP) loci in the genomic DNA of the golden ear fungus germplasm, comparing it with the golden ear fungus DNA fingerprint database in Table 4, calculating genetic similarity, and identifying the golden ear fungus germplasm. Detailed results are available in [link to results]. Figure 8 Genotyping results of D13A and 3-13, D25A and 3-25, D40A and 3-40, and D47A and 3-47 golden ear materials. Figure 13 Genetic similarity results of D13A and 3-13, D25A and 3-25, D40A and 3-40, D47A and 3-47 golden ear materials. Figure 8 In the diagram, each row represents one data set, and each column represents one SNP genotype. Blue, red, purple, and orange represent alleles C / C, A / A, G / G, and T / T, respectively. Unknown alleles are shown in white, and alleles are represented by 0 / 0; heterozygous sites are shown in gray. For clearer visualization... Figure 8 ,Will Figure 8 Segmented magnification, divided into Figure 9 , Figure 10 , Figure 11 and Figure 12 exhibit. Figure 13 In the diagram, genetic similarity changes from blue to red.

[0050] Taking samples 3-13, 3-25, 3-40, and 3-47 as examples, strain information is shown in Table 5. Sample DNA was re-extracted and named D13A, D25A, D40A, and D47A. Whole-genome resequencing was performed on these four samples, and the genotypes of 108 MNPs (333 SNPs) were extracted. The results showed that most of the alleles detected in D13A were identical to those in 3-13, D25A to 3-25, D40A to 3-40, and D47A to 3-47. Subsequently, the genetic similarity was calculated using the formula GS = n / N × 100%, where N is the number of MNP loci, and n is the number of MNP loci with the same genotype for each Golden Ear germplasm resource or variety. For example, D13A and 3-13, the number of MNP loci mentioned by N is 108, including 333 SNPs. Each SNP locus has 2 alleles, for a total of 666 alleles. n is the number of SNP loci with the same genotype when comparing D13A and 3-13. There are 664 identical alleles, that is, the genetic similarity GS is 664 / 666×100%=99.70%. Therefore, the genetic similarity of D13A and 3-13 is 99.70%. D25A and 3-25, D40A and 3-40, and D47A and 3-47 are calculated using the same method, and the genetic similarity GS are 99.85%, 99.70%, and 99.40%, respectively. It is determined that D13A and 3-13, D25A and 3-25, D40A and 3-40, and D47A and 3-47 are all of the same type, which is consistent with the facts.

[0051] Table 4. Variations of D13A and 3-13, D25A and 3-25, D40A and 3-40, D47A and 3-47 in the golden ear fungus

[0052] DNA fingerprinting

[0053] Serial Number name DNA fingerprinting 1 3-13 T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A C / C T / T A / A T / T A / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / CA / A A / A G / G C / C C / C A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / G A / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C T / T T / TC / C C / C G / G G / G T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / G T / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / CT / T A / A T / T G / G C / C T / T T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / T G / G A / A T / T C / C T / C T / T G / G T / T G / G T / T T / TC / C T / T T / T T / T G / G C / C C / C C / C G / G C / C G / G G / G T / T C / C G / G A / A G / G T / T G / G C / C A / A G / G A / A C / C C / C G / G T / T T / T C / CG / G A / A A / A T / T A / A A / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A A / A T / T C / C A / A C / C C / C T / T C / C G / G C / C G / G A / A A / AG / G C / C T / T G / G C / C C / C G / G T / T C / C T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G G / G T / T G / G G / G C / C G / G A / A A / A G / GC / C A / A T / T A / A T / T G / G A / A C / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / T G / G G / G G / G G / G A / A G / G T / TC / C G / G T / T T / T G / G G / G C / C A / A A / A T / T G / G T / T A / A T / T G / G A / A G / G C / C G / G A / AT / T A / A T / T T / T T / T A / A C / C T / T A / AC / C C / C C / C C / C C / C G / G T / T T / T A / A C / G A / G C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / G C / C G / G G / G A / A C / CC / C A / A T / T T / T A / A C / C A / A G / G T / T T / T A / A G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C T / T C / C C / C C / C T / T G / GT / T G / G G / G A / A C / C A / A G / G G / G G / G C / C A / A C / C G / G C / C 2 3-25 C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / T A / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / CA / A A / A G / G C / C C / C A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / G A / A A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / CG / G T / T C / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / A C / C T / T G / G A / A G / G T / T C / C A / A A / A T / T T / T C / CT / T A / A T / T G / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T G / G A / A T / T G / G A / A T / T C / C T / C T / T G / G T / T G / G T / T T / TC / C T / T T / T T / T T / T G / G T / T T / T C / C T / T A / A A / A C / C G / G T / T G / G A / A G / G T / T A / A C / C T / T C / C T / T T / T G / G T / T T / T C / CG / G A / A A / A C / C T / T C / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / A C / C C / C T / T T / T A / A T / T A / A G / G C / CA / A G / G T / T G / G C / C C / C G / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / G T / T G / G G / G C / C G / G A / A A / A A / AT / T A / A T / T G / G G / G G / G A / A C / C G / G A / A C / C C / C T / T A / A T / T A / A T / T G / G T / T T / T A / A T / T G / G G / G G / G G / G A / A A / A C / CT / T A / A C / C C / C T / T T / T T / T G / G G / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / AT / T A / A T / T T / T T / T A / A C / C T / T A / AC / C C / C C / C T / T T / T G / G T / T T / T A / A C / C A / A C / C T / T G / G T / T G / G A / A C / C A / A C / C T / T A / A T / T A / A A / A A / A C / C T / T T / TT / T G / G C / C C / C G / G C / C A / A G / G T / T T / T A / A C / C C / C A / A T / T C / C G / G A / A T / T C / C C / C C / C T / T T / T C / C C / C C / C T / T G / GT / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / G G / G A / A T / T 3 3-40 C / C G / G G / G C / C C / C C / C C / C G / G C / C A / A G / G C / C T / T A / A T / T A / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / CA / A A / A G / G C / C C / C A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / G A / A G / G C / C T / T C / C G / G T / T G / G T / T A / A C / C T / T T / TC / C C / C G / G G / G C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / G T / T A / A T / T G / G T / T T / T C / C A / A A / A T / T C / C A / AC / C G / G C / C A / A G / G G / G C / C C / C A / A A / A C / C A / A A / A G / G C / C G / G A / A T / T G / G A / A 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A T / T T / TC / C T / T T / T T / T G / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / T A / A C / C T / T C / C C / C C / C G / G T / T T / T C / CG / G A / A A / A T / T A / A A / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A A / A T / T C / C A / A C / C C / C T / T C / C G / G C / C G / G A / A A / AG / G C / C T / T G / G C / C C / C G / G T / T C / C T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / C A / A A / A T / T G / G A / A C / C T / T A / AT / T A / A T / T G / G G / G G / G A / A C / C A / A G / G G / G A / A C / C G / G C / C A / A T / T A / A A / A A / A C / C A / A G / G G / G G / G G / G A / A G / G T / TC / C G / G T / T T / T G / G G / G T / T G / G G / G C / C G / G T / T A / A T / T G / G A / A G / G C / C G / G G / GC / C C / C C / C C / C C / C G / G T / T C / C C / CT / T T / T G / G C / C C / C G / G T / T T / T A / A C / C A / A C / T T / G G / G T / T G / G A / A C / C C / C A / A T / T A / A T / T A / A A / A A / A C / C T / T T / TT / T G / G C / C T / T A / A A / A G / G A / A C / C G / G G / G G / G T / T 0 / 0 0 / 0 0 / 0 G / G A / A T / T T / T T / T G / G C / C C / C T / T A / A T / T C / C A / AG / G T / T C / C G / G C / C A / A G / G G / G G / G C / C G / G G / G A / A T / T 4 3-47 T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G C / C T / T A / A T / T A / A G / G T / T A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / TC / C C / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / G A / A G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T C / C C / CG / G T / T C / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / G T / T A / A T / T A / A G / G C / C G / G G / G C / C A / A C / C A / AT / T A / A T / T G / G C / C T / T C / C C / C A / A A / A T / T T / T G / G C / C T / T A / A G / G C / C G / G A / A T / A C / T T / C C / C T / T A / A A / A C / C G / GC / C T / T T / T T / T T / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / G C / C A / A G / G A / A C / C C / C G / G T / T T / T C / CG / G A / A A / A T / T A / A A / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A A / A T / T C / C A / A C / C C / C T / T T / T A / A T / T A / A G / G C / CA / A G / G A / A A / A T / T T / T A / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / C A / A A / A T / T G / G A / A C / C T / T G / GC / C C / C G / G A / A T / T G / G A / A C / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / T T / T T / T A / A A / A C / C A / A C / CT / T A / A C / C C / C T / T T / T T / T G / G G / G C / C A / A G / G G / G C / C A / A G / G A / A T / T A / A A / AT / T C / C C / C C / C C / C G / G T / T C / C C / CT / T T / T G / G T / T T / T G / G T / T T / T A / A C / G A / G C / T T / G G / G T / T G / G A / A C / C C / C A / A A / A T / T T / T A / A A / A G / G G / G A / A C / CC / C A / A T / T T / T A / A A / A G / G A / A C / C G / G G / G G / G T / T A / A T / A C / T G / G A / A T / T T / T T / T G / G C / C C / C T / T A / A T / T C / C A / AG / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / A C / C G / G C / C 5 D13A T / T A / A A / A T / T T / T T / T T / T C / C G / G C / C A / A C / C T / T A / A T / T A / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / CA / A A / A G / G C / C C / C A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / G A / A G / G C / C C / C T / T A / A C / C G / G T / T A / A C / C T / T T / TC / C C / C G / G G / G T / T G / G G / G A / A C / C C / C A / A T / T G / G A / A C / C T / T G / G T / T A / A T / T G / G T / T T / T C / C A / A A / A T / T T / T C / CT / T A / A T / T G / G C / C T / T T / T T / T G / G G / G T / T T / T A / A G / G C / C G / G A / A T / T G / G A / A T / T C / C T / C T / T G / G T / T G / G T / T T / TC / C T / T T / T T / T G / G C / C C / C C / C G / G C / C G / G G / G T / T C / C G / G A / A G / G T / T G / G C / C A / A G / G A / A C / C C / C G / G T / T T / T C / CG / G A / A A / A T / T A / A A / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A A / A T / T C / C A / A C / C C / C T / T C / C G / G C / C G / G A / A A / AG / G C / C T / T G / G C / C C / C G / G T / T C / C T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G G / G T / T G / G G / G C / C G / G A / A A / A G / GC / C A / A T / T A / A T / T G / G A / A C / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / T G / G G / G G / G G / G A / A G / G T / TC / C G / G T / T T / T G / G G / G C / C A / A A / A T / T G / G T / T A / A T / T G / G A / A G / G C / C G / G A / AT / T A / A T / T T / T T / T A / A C / C T / T A / AC / C C / C C / C C / C C / C G / G T / T T / T A / A C / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A T / T A / A C / C G / G C / C G / G G / G A / A C / CC / C A / A T / T T / T A / A C / C A / A G / G T / T T / T A / A G / G T / T T / A T / T C / C C / C G / G C / C T / T T / T G / G C / C T / T C / C C / C C / C T / T G / GT / T G / G G / G A / A C / C A / A G / G G / G G / G C / C A / A C / C G / G C / C 6 D25A C / C G / G G / G C / C C / C C / C C / C C / C G / G C / C A / A C / C T / T A / A T / T A / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / CA / A A / A G / G C / C C / C A / A C / C A / A G / G G / G C / C T / T C / C T / T T / T G / G A / A A / A T / T C / C T / T A / A C / C G / G T / T A / A C / C C / C C / CG / G T / T C / C T / T C / C C / C A / A C / C T / T A / A T / T A / A C / C G / G G / G C / C A / A C / C T / T G / G A / A G / G T / T C / C A / A A / A T / T T / T C / CT / T A / A T / T G / G C / C T / T C / C C / C A / A A / A C / C A / A G / G C / C T / T G / G A / A T / T G / G A / A T / T C / C T / T T / T G / G T / T G / G T / T T / TC / C T / T T / T T / T T / T G / G T / T T / T C / C T / T A / A A / A C / C G / G T / T G / G A / A G / G T / T A / A C / C T / T C / C T / T T / T G / G T / T T / T C / CG / G A / A A / A C / C T / T C / C A / A C / C G / G G / G G / G A / A C / C G / G C / C A / A A / A T / T C / C A / A C / C C / C T / T T / T A / A T / T A / A G / G C / CA / A G / G T / T G / G C / C C / C G / G T / T C / C C / C G / G G / G G / G G / G G / G G / G G / G T / T G / G A / A G / G T / T G / G G / G C / C G / G A / A A / A A / AT / T A / A T / T G / G G / G G / G A / A C / C G / G A / A C / C C / C T / T A / A T / T A / A T / T G / G T / T T / T A / A T / T G / G G / G G / G G / G A / A A / A C / CT / T A / A C / C C / C T / T T / T T / T G / G G / G C / C G / G T / T A / A T / T G / G G / G A / A T / T A / A A / AT / T A / A T / T T / T T / T A / A C / C T / T A / AC / C C / C C / C T / T T / T G / G T / T T / T A / A C / C A / A C / C T / T G / G T / T G / G A / A C / C A / A C / C T / T A / A T / T A / A A / A A / A C / C T / T T / TT / T G / G C / C C / C G / G C / C A / A G / G T / T T / T A / A C / C C / C A / A T / T C / C G / G A / A T / T C / C C / C C / C T / T T / T C / C C / C C / C T / T G / GT / T G / G C / C G / G T / T G / G A / A A / A A / A T / T G / G G / G A / A T / T 7 D40A C / C G / G G / G C / C C / C C / C C / C G / G C / C A / A G / G C / C T / T A / A T / T A / A G / G T / T T / T C / C T / T C / C T / T T / T C / C G / G C / C A / A C / CA / A A / A G / G C / C C / C A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / G A / A G / G C / C T / T C / C G / G T / T G / G T / T A / A C / C T / T T / TC / C C / C G / G G / G C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / G T / T A / A T / T G / G T / T T / T C / C A / A A / A T / T C / C A / AC / C G / G C / C A / A G / G G / G C / C C / C A / A A / A C / C A / A A / A G / G C / C G / G A / A T / T G / C A / C 0 / 0 0 / 0 0 / 0 C / C T / T A / A A / A T / T T / TC / C T / T T / T T / T G / G C / C C / C C / C G / G C / C A / A A / A C / C G / G T / T G / G A / A G / G T / T A / A C / C T / T C / C C / C C / C G / G T / T T / T C / CG / G A / A A / A T / T A / A A / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A A / A T / T C / C A / A C / C C / C T / T C / C G / G C / C G / G A / A A / AG / G C / C T / T G / G C / C C / C G / G T / T C / C T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / C A / A A / A T / T G / G A / A C / C T / T A / AT / T A / A T / T G / G G / G G / G A / A C / C A / A G / G G / G A / A C / C G / G C / C A / A T / T A / A A / A A / A C / C A / A G / G G / G G / G G / G A / A G / G T / TC / C G / G T / T T / T G / G G / G T / T G / G G / G C / C G / G T / T A / A T / T G / G A / A G / G C / C G / G G / GC / C C / C C / C C / C C / C G / G T / T C / C C / CT / T T / T G / G C / C C / C G / G T / T T / T A / A C / C A / A C / T T / G G / G T / T G / G A / A C / C C / C A / A T / T A / A T / T A / A A / A A / A C / C T / T T / TT / T G / G C / C T / T A / A A / A G / G A / A C / C G / G G / G G / G T / T 0 / 0 0 / 0 0 / 0 G / G A / A T / T T / T T / T G / G C / C C / C T / T A / A T / T C / C A / AG / G T / T C / C G / G C / C A / A G / G G / G G / G C / C G / G G / G A / A T / T 8 D47A T / T A / A A / A T / T T / T T / T T / T G / G C / C A / A G / G C / C T / T A / A T / T A / A G / G T / T A / A G / G G / G A / A G / G C / C T / T A / A G / G C / C T / TC / C C / C A / A T / T A / A A / A C / C G / G T / T A / A G / G G / G A / A G / G C / C G / G A / A G / G C / C C / C T / T A / A C / C A / A C / C G / G T / T C / C C / CG / G T / T C / C T / T C / C C / C A / A A / A C / C C / C A / A T / T G / G A / A C / C T / T G / G T / T A / A T / T A / A G / G C / C G / G G / G C / C A / A C / C A / AT / T A / A T / T G / G C / C T / T C / C C / C A / A A / A T / T T / T G / G C / C T / T A / A G / G C / C G / G A / A T / A C / T T / C C / C T / T A / A A / A C / C G / GC / C T / T T / T T / T T / T G / G T / T T / T C / C T / T G / G G / G T / T C / C G / G A / A G / G T / T G / G C / C A / A G / G A / A C / C C / C G / G T / T T / T C / CG / G A / A A / A T / T A / A A / A G / G A / A A / A C / C A / A G / G T / T A / A C / C A / A A / A T / T C / C A / A C / C C / C T / T T / T A / A T / T A / A G / G C / CA / A G / G A / A A / A T / T T / T A / A G / G T / T T / T C / C A / A A / A A / A A / A A / A T / T C / C A / A G / G C / C A / A A / A T / T G / G A / A C / C T / T G / GC / C C / C G / G A / A T / T G / G A / A C / C A / A G / G G / G A / A C / C G / G C / C G / G C / C G / G T / T T / T A / A T / T T / T T / T A / A A / A C / C A / A C / CT / T A / A C / C C / C T / T T / T T / T G / G G / G C / C A / A G / G G / G C / C A / A G / G A / A T / T A / A A / AT / T C / C C / C C / C C / C G / G T / T C / C C / CT / T T / T G / G T / T T / T G / G T / T T / T A / A C / C A / A C / C T / T G / G T / T G / G A / A C / C C / C A / A A / A T / T T / T A / A A / A G / G G / G A / A C / CC / C A / A T / T T / T A / A A / A G / G A / A C / C G / G G / G G / G T / T A / A T / A C / T G / G A / A T / T T / T T / T G / G C / C C / C T / T A / A T / T C / C A / AG / G T / T G / G A / A C / C A / A G / G G / G G / G C / C A / A C / C G / G C / C

[0054] Table 5. Information on strains of *Auricularia auricula-judae* D13A and 3-13, D25A and 3-25, D40A and 3-40, and D47A and 3-47.

[0055] Serial Number strain strain type source 1 3-13 Single spore Yunnan Junshijie Biotechnology Co., Ltd. 2 3-25 Single spore Yunnan Junshijie Biotechnology Co., Ltd. 3 3-40 Single spore Yunnan Junshijie Biotechnology Co., Ltd. 4 3-47 Single spore Yunnan Junshijie Biotechnology Co., Ltd. 5 D13A 3-13 Obtained through asexual reproduction Yunnan Junshijie Biotechnology Co., Ltd. 6 D25A Obtained through asexual reproduction on March 25th. Yunnan Junshijie Biotechnology Co., Ltd. 7 D40A Obtained through asexual reproduction between 3 and 40 Yunnan Junshijie Biotechnology Co., Ltd. 8 D47A Obtained through asexual reproduction between 3 and 40 Yunnan Junshijie Biotechnology Co., Ltd.

[0056] The foregoing description of specific exemplary embodiments of the present invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various exemplary embodiments of the invention, as well as various choices and modifications. Therefore, the scope of protection of the present invention is not limited to the embodiments.

[0057] The MNP molecular markers and the constructed *Gynostemma pentaphyllum* DNA map provided by this invention can be applied to any of the following aspects:

[0058] (1) Used for constructing DNA fingerprinting of golden ear fungus;

[0059] (2) Used for genotyping of golden ear fungus;

[0060] (3) Used for the identification of golden ear germplasm resources and varieties;

[0061] (4) Used for the breeding of golden ear germplasm resources;

[0062] (5) Used for molecular marker-assisted breeding of golden ear fungus.

Claims

1. A method for constructing a DNA fingerprint of *Gynostemma pentaphyllum* using a combination of molecular markers located in the missense coding region (MNP), characterized in that... The MNP molecular marker assemblage consists of 108 MNP molecular markers, numbered Na-001 to Na-108, with Ns_27 as the reference genome and NCBI project number: PRJNA1264744; Molecular marker information is as follows: ； In the above information, the markers are interpreted as follows: The molecular marker with MNP number Na-001 is located on chromosome 1, with physical positions from 157077 to 157177, containing 7 SNPs at physical positions 157077, 157090, 157091, 157092, 157099, 157100, and 157140. The alleles corresponding to these physical positions are T / C, A / G, A / G, T / C, T / C, T / C, and T / C, respectively. Among these, 157077, 157091, 157092, 157100, and 157140 are missense coding regions, encoding the gene NAU27000055. The interpretations of the other molecular markers are similar to those of the Na-001 molecular marker. The method for constructing a DNA fingerprint of *Eurydon cylindrica* using the MNP molecular marker combination is as follows: S1. Extract DNA from the golden ear germplasm resources or varieties used to construct fingerprint maps and detect the genotypes of 108 MNP loci from Na-001 to Na-108. S2. For each Golden Ear germplasm resource or variety, the genotypes at the 108 MNP loci are combined and sorted according to the locus numbering order to form a specific sequence. This specific sequence is the Golden Ear DNA fingerprint map. The map number, name, and DNA fingerprint are as follows:

2. A method for identifying *Auricularia auricula-judae* varieties using a DNA fingerprint constructed according to claim 1, characterized in that, DNA was extracted from the spores of the *Auricularia auricula-judae* germplasm resources or varieties to be tested. The genotypes of 108 MNP loci (Na-001~Na-108) in the genomic DNA of the germplasm were detected. The genotyping of the MNP molecular markers was determined based on the MNP loci detection results. The results were compared with the constructed DNA fingerprint and the genetic similarity GS was calculated. When the genetic similarity GS of the *Auricularia auricula-judae* germplasm resources or varieties to be tested was greater than or equal to 96%, they were identified as the same germplasm or variety. The genetic similarity GS = n / N × 100%, where N is the number of MNP loci, and n is the number of MNP loci with the same genotype for each *Auricularia auricula-judae* germplasm resource or variety.