Cultivation method for regulating and controlling excellent characters of Korean pine seedlings by using plant hormones

Abstract

The invention belongs to the technical field of plant cultivation, and particularly relates to a cultivation method for regulating and controlling excellent characters of Korean pine seedlings through plant hormones. The invention discloses a cultivation method for regulating and controlling excellent characters of Korean pine seedlings based on DA-6. According to the method, aiming at Korean pine seedlings of different seedling ages, a plant growth regulator DA-6 with a specific concentration is adopted for spraying, and foliage spraying is conducted three times in July to August of the vigorous growth period of the seedlings. The result of the embodiment shows that the method can remarkably promote the growth of the overground part and the root system of the Korean pine seedling, effectively solves the technical problems of slow growth, long cultivation period and short supply of the Korean pine seedling, and has the advantages of simplicity and convenience in operation, low cost, remarkable effect and environmental friendliness.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of plant cultivation, and particularly relates to a cultivation method for regulating excellent traits of Korean pine seedlings by using plant hormones. BACKGROUND

[0002] Korean pine (Pinus koraiensis Sieb. et Zucc.) is a kind of evergreen coniferous tree, belonging to Pinaceae and Pinus. Pinus koraiensis Pinaceae Spreng Pinus L.

[0003] Korean pine is a national second-class protected plant, with a long life span and a tall tree shape, which can be as high as 40 meters or more, with a straight trunk and a conical crown. Its leaves are needle-shaped, long and narrow. It is cold-resistant and can adapt to the cold winter climate in the northeast. As a pioneer species, it can establish a stable community through natural succession and has outstanding ecological carrying capacity in the northeast.

[0004] As a valuable economic tree, Korean pine is an excellent timber tree species. Its sapwood is light yellow white, and its heartwood is light yellow brown or light brown red. It is light and soft, with straight texture and fine structure, and has high density, high hardness, low water absorption rate and strong corrosion resistance, and is widely used in the manufacture of furniture, bridges and ships. It contains rich polyphenols and resins, which can be used as industrial raw materials for food, cosmetics, medicine and high polymer materials. Korean pine seeds contain oil, phenols and amino acids, and have high edible value. The pine polyphenols in pine nuts also have many biomedical effects such as free radical scavenging, anti-inflammatory, antibacterial and blood sugar lowering.

[0005] More attention should be paid to the fact that Korean pine has a very high ecological value, which is more than 1300 times the economic value, mainly in terms of carbon absorption and oxygen emission, climate regulation, water conservation, wind and sand prevention, species diversity protection and national security protection. It has broad development prospects and great development potential, and is an important tree species with unique characteristics in China.

[0006] Since the 1950s, over-exploitation of forests has led to the large-scale cutting of original broad-leaved Korean pine forests, and many generations of natural secondary forests and plantations have been formed. The secondary forests of Korean pine in the northeast are mainly formed by human disturbance.

[0007] Korean pine is a semi-sun positive shallow-rooted tree species, which prefers light and requires high soil moisture and atmospheric humidity. It is intolerant to dry and wet soil and severe cold. It grows best in warm and cold and humid conditions with a humidity of 0.7 or more, and in deep and fertile and well-drained acid brown forest soil. Young trees can tolerate shade and are mainly produced in the corresponding environment with an altitude of 150-1800 meters in the northeast Changbai Mountain area, Jilin Mountain area and Aihui in the Xiaoxing'an Mountains.

[0008] ​​​Plant growth regulators refer to hormones or biological and chemical agents artificially synthesized or extracted from organisms that have the function of regulating the growth and development process of plants. Plant growth regulators play an important role in the growth and development process of plants. Plant growth regulators can break seed dormancy, improve seed germination rate, and promote plant growth and development.

[0009] DA-6 (diethylaminoethanol hexanoate, N,N-diethyl aminoethyl hexanoate ) is a plant growth regulator with ultra-high biological activity, which is artificially synthesized. DA-6 belongs to tertiary amine substances in chemical classification and is generally referred to as fresh amine ester in production. At present, DA-6 has been widely used in various economic crops in agriculture and forestry. When used in combination with other plant growth regulators, it can increase the chlorophyll content in plant leaves and the sugar and protein content in fruits, and also improve the photosynthetic rate and carbon and nitrogen metabolism of plants, thereby increasing the yield and improving the quality of crops. DA-6 is a plant growth regulator with universality and significant effect. It is widely welcomed because of its advantages such as high efficiency, convenience, green, ecological environmental protection, fast degradation, no residue, resistance to adversity and disease, broad-spectrum efficacy, long-term use, low cost, high efficiency, etc.

[0010] Therefore, in view of the fact that the growth cycle of Korean pine seedlings is generally long, the current market has shown a clear situation of supply not meeting demand. Under this background, there is an urgent need for a cultivation method, the core goal of which is to accelerate the lignification process of Korean pine seedlings, accurately regulate the plant type structure, effectively improve the cultivation yield, and ultimately realize the large-scale cultivation of Korean pine seedlings with excellent traits. SUMMARY

[0011] The purpose of the present application is to provide a cultivation method for regulating the excellent traits of Korean pine seedlings. The method provided by the present application solves the problems of difficult operation, long cycle and high cost in cultivating excellent Korean pine seedlings by traditional methods.

[0012] In order to achieve the above-mentioned purpose, the present application provides the following technical solutions: The present application provides a cultivation method for regulating the excellent traits of Korean pine seedlings, which comprises the following steps: taking Korean pine seedlings as the spraying object, applying plant hormone DA-6 to the needle leaves and stem of the Korean pine seedlings during the vigorous growth period, and spraying uniformly until the needle leaves drip water.

[0013] Preferably, the spraying object is 2-0 two-year-old seedlings, and the seedlings that have not been transplanted or 2-1 two-year-old seedlings are transplanted once, and the three-year-old seedlings are cultivated for one year after transplantation.

[0014] Preferably, the vigorous growth period is from July to August.

[0015] Preferably, the spraying is performed three times, and the interval time of the spraying times is 11-15 days.

[0016] Preferably, the concentration of the applied plant hormone DA-6 is selected according to the type of seedling age of Korean pine seedlings: a first concentration range is applied to 2-0 type seedlings, and a second concentration range is applied to 2-1 type seedlings.

[0017] Preferably, the first concentration range is 8-12 mg / L, and the second concentration range is 25-35 mg / L.

[0018] Preferably, a DA-6 solution with a concentration of 10 mg / L is applied to 2-0 type seedlings.

[0019] Preferably, a DA-6 solution with a concentration of 30 mg / L is applied to 2-1 type seedlings.

[0020] Preferably, the spraying device uses a backpack sprayer or an electric sprayer.

[0021] Compared with the prior art, the present application has the following beneficial effects: 1) The present application determines the type and optimal concentration of plant regulators suitable for Korean pine seedlings through experiments, solving the problems of difficult operation, long cycle and high cost in traditional methods for cultivating fine Korean pine seedlings.

[0022] 2) The present application applies plant growth regulator DA-6 to the cultivation of Korean pine seedlings, and determines the optimal spraying concentration for Korean pine seedlings of different ages through experiments. This scheme can significantly promote the growth and development of Korean pine seedlings, specifically in the comprehensive promotion of multiple key growth indicators such as stolon, seedling height, new shoot length, aboveground biomass (fresh weight and dry weight), root biomass (main root and lateral root dry weight), and root architecture (main root length, number of Ⅰ and Ⅱ roots), thereby efficiently cultivating high-quality seedlings with excellent traits.

[0023] 3) This method only needs to be sprayed during the seedling growth season, without the need for complex equipment or high-technology operation processes, and is easy to implement in large nurseries. DA-6 regulator itself has low cost and small dosage, making the overall investment cost of this method low and the economic benefit high. BRIEF DESCRIPTION OF DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0025] Figure 1Figure for determination results of multiple growth indexes of 2-0 type Korean pine seedlings treated by DA-6 at different concentrations, including ground stem, crown width, seedling height, new shoot length, aboveground fresh weight, aboveground dry weight, root fresh weight, root dry weight, main root dry weight, lateral root dry weight, main root length, Ⅰ level root length, Ⅰ level root number, Ⅱ level root number, root width and the like; data is mean ± standard error (±SE); according to Duncan multiple range test, letters represent significance of test concentration (p<0.05); A and B are changes of DA-6 aboveground part indexes, C, D, E and F are changes of DA-6 underground part indexes.

[0026] Figure 2 Figure for determination results of multiple growth indexes of 2-1 type Korean pine seedlings treated by DA-6 at different concentrations, including ground stem, crown width, seedling height, branch diameter, new shoot length, lateral branch number, lateral branch length, aboveground fresh weight, aboveground dry weight, root fresh weight, root dry weight, main root dry weight, lateral root dry weight, main root length, Ⅰ level root length, Ⅰ level root number, Ⅱ level root number, root width and the like; data is mean ± standard error (±SE); according to Duncan multiple range test, letters represent significance of test concentration (p<0.05); A, B and C are changes of DA-6 aboveground part indexes, D, E and F are changes of DA-6 underground part indexes.

[0027] Figure 3 Figure for DA-6 spraying test at different concentrations.

[0028] Figure 4 Figure for phenotype of 2-0 type Korean pine seedlings treated by DA-6.

[0029] Figure 5 Figure for phenotype of 2-0 type Korean pine seedlings in a control group (CK).

[0030] Figure 6 Figure for phenotype of 2-1 type Korean pine seedlings treated by DA-6.

[0031] Figure 7 Figure for phenotype of 2-1 type Korean pine seedlings in a control group (CK). DETAILED DESCRIPTION

[0032] In order to further illustrate the present application, the technical solutions provided by the present application are described in detail below in combination with the drawings and examples, but they should not be understood as limiting the scope of protection of the present application.

[0033] The production process, experimental method or detection method involved in the embodiments of the present application, if no special description, are conventional methods in the prior art, and the name and / or abbreviation thereof belong to the conventional name in the art, which is very clear and explicit in the related application field, and the person skilled in the art can understand the conventional process steps and apply the corresponding equipment according to the name, and implement according to the conventional conditions or the conditions recommended by the manufacturer.

[0034] The various instruments, equipment, raw materials or reagents used in the embodiments of the present application have no special restriction on the source, and are conventional products that can be purchased through normal commercial channels, or can be prepared according to the conventional method well known to those skilled in the art.

[0035] Example 1 Experimental time and place: July to August, Jingshantun nursery in Yichun City, Heilongjiang Province.

[0036] Experimental materials: 2-0 type Korean pine seedlings (two-year-old non-transplanted seedlings) with uniform growth were selected.

[0037] Experimental design: A completely randomized block design was used, and five DA-6 concentration gradient treatment groups were set: 5 mg / L, 10 mg / L, 15 mg / L, 20 mg / L, and 30 mg / L, with water treatment as a control (CK). Each treatment was sprayed on 60 plants, and a total of 360 Korean pine seedlings were sprayed, as shown in Figure 3

[0038] Spraying method: Spraying was carried out three times on July 2, July 16, and July 30, using a backpack sprayer to uniformly spray the DA-6 solution of the corresponding concentration on the needle leaves and stems of the seedlings, so that the leaves were wet and water droplets were just dripping. Spraying was carried out in the evening on a sunny and windless day.

[0039] The Korean pine seedlings after DA-6 spraying were compared with the control, and the results of variance analysis are shown in Table 1 and Figure 1

[0040] After 30 days of the last spraying, 10 seedlings were randomly selected from each group, and the ground stem, seedling height, new shoot length, aboveground and root biomass, and root morphology index were measured. Single factor variance analysis was performed using SPSS software, and Duncan multiple comparison test was used to test the difference between different treatments (significance level p<0.05). The results of variance analysis are shown in Table 1 and Figure 1

[0041] Table 1 Variance analysis results of different DA-6 treatments on 2-0 Korean pine seedling growth indexes

[0042] ​​​Results: Analysis of variance showed that different concentrations of DA-6 treatment on 2-0 Korean pine seedlings, ground stem, height, new shoot length, aboveground fresh weight and dry weight, root dry weight, main root dry weight, lateral root dry weight, main root length, I class root number, II class root number and other key growth indicators have a very significant (p<0.01) or significant (p<0.05) effect.

[0043] Multiple comparison analysis as shown in Figure 1 , 10 mg / L DA-6 treatment group in most indicators are significantly better than the control group (CK), and the comprehensive promotion effect in all treatment groups is the most prominent, determined as the best spraying concentration of 2-0 type Korean pine seedlings.

[0044] As shown in Figure 4 and Figure 5 , the Korean pine seedlings treated with 10 mg / L DA-6 are significantly better than the water control group in plant height, stem thickness, root development and overall growth, fully demonstrating the significant effect of the present application in promoting the lignification of Korean pine seedlings, optimizing plant type and accelerating growth.

[0045] Example 2 Experimental time and place: same as example 1.

[0046] Experimental materials: 2-1 type Korean pine seedlings (three-year-old seedlings after one year of growth after transplanting once) were selected.

[0047] Experimental design: the experimental design is the same as example 1, and 5 DA-6 concentration gradient treatment groups are set as 5, 10, 15, 20, 30 mg / L, and water control. Each treatment has 60 seedlings.

[0048] Spraying method: three times of spraying were carried out on the same date, and the spraying time, frequency, equipment and operation standard were the same as example 1. The method is the same as example 1. The determination indexes include ground stem, crown width, height, new shoot length, aboveground and root biomass, root morphology, etc. The data is subjected to analysis of variance and Duncan multiple comparison. The results of analysis of variance are shown in table 2.

[0049] Table 2 Analysis of variance of DA-6 on 2-1 Korean pine seedling growth indexes

[0050] The analysis results show that different concentrations of DA-6 treatment on 2-1 Korean pine seedlings, ground stem, crown width, height, new shoot length, aboveground fresh weight and dry weight, root fresh weight and dry weight, main root dry weight, lateral root dry weight, I class root number, II class root number, root width and other key growth indicators have a very significant (p<0.01) effect.

[0051] Multiple comparison analysis shows (see Figure 2), the 30 mg / L DA-6 treatment group is significantly better than the control group (CK) and other concentration treatment groups in most indexes, and is determined as the best spraying concentration of 2-1 type Korean pine seedlings.

[0052] As shown in Figure 6 and Figure 7 the Korean pine seedlings treated by 30 mg / L DA-6 are significantly better than the water control group in crown formation, plant height growth, stem thickness and root development, which directly proves the excellent effect of the present application in optimizing the plant type, promoting lignification and accelerating growth of Korean pine seedlings.

[0053] Although the above embodiment has made a detailed description of the present application, it is only a part of the embodiments of the present application, but not all the embodiments, and other embodiments can be obtained according to the present embodiment without creativity, which all belong to the protection scope of the present application.

Claims

1. A method for cultivating superior traits of Korean pine seedlings, characterized in that, The method includes the following steps: taking Korean pine seedlings as the target of spraying, during the vigorous growth period of the Korean pine seedlings, the plant hormone DA-6 is applied and sprayed evenly on the needles and stems of the Korean pine seedlings until the needles drip water.

2. The method according to claim 1, characterized in that, The spraying targets are 2-0 two-year seedlings that have not been transplanted, or 2-1 two-year seedlings that have been transplanted once and then cultivated for one more year as three-year seedlings.

3. The method according to claim 1, characterized in that, The peak growth period is from July to August.

4. The method according to claim 1, characterized in that, The spraying was performed three times, with an interval of 11-15 days between each spraying.

5. The method according to claim 1, characterized in that, The concentration of the plant hormone DA-6 applied is selected according to the age type of the Korean pine seedlings: the first concentration range is applied to 2-0 type seedlings, and the second concentration range is applied to 2-1 type seedlings.

6. The method according to claim 5, characterized in that, The first concentration range is 8-12 mg / L, and the second concentration range is 25-35 mg / L.

7. The method according to claim 5, characterized in that, Apply a DA-6 solution at a concentration of 10 mg / L to 2-0 type seedlings.

8. The method according to claim 5, characterized in that, Apply a DA-6 solution at a concentration of 30 mg / L to type 2-1 seedlings.

9. The method according to claim 1, characterized in that, The spraying equipment is a backpack sprayer or an electric sprayer.

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