Nuciferine research experimental mouse feeding control system

Abstract

The application discloses a kind of experimental mouse feeding control system applied to nuciferine research, it is related to intelligent experimental control technical field, it includes control module and the indicator light, detection mechanism and display electrically connected to control module, detection mechanism is installed in breeding cage and is used to detect feeding behavior, it includes mesh plate and weighing sensor, load-bearing platform is arranged below mesh plate, weighing sensor is installed on load-bearing platform, mesh plate is located on weighing sensor, feed trough in breeding cage is located on mesh plate, mesh plate is placed back to position after experimental mouse is taken out;Indicator light is two and is respectively installed in breeding cage, indicator light is RGB lamp type, control module is based on the weight data fed back by weighing sensor and whether the color of indicator light is switched within the preset time length to control indicator light to switch color and prompt.The present application has the effect of improving the success rate of nuciferine animal experiment.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of intelligent experiment control, in particular to an experimental mouse feeding control system applied to nuciferine research. BACKGROUND

[0002] With the continuous improvement of people's living standards and the increase of Internet entertainment and office time, obesity is becoming a new problem threatening people's health. Relevant agencies and personnel believe that nuciferine in lotus leaves has a good effect on preventing obesity, so it is necessary to carry out research on it. Such research is generally carried out through experimental mice.

[0003] In the above research, DIO (high-fat diet-induced) mice, ob / ob (leptin gene mutant) mice, db / db (leptin receptor gene mutant) mice and other animals are needed, and need to be divided into multiple groups for long-term cultivation and observation by different feeding methods. Only relying on labels, tables and other records, staff are prone to feeding, injection time differences and other omissions, and there is a certain probability of mouse disease and death during the 6-12 week long-term cultivation process, resulting in experimental delay and failure. Therefore, a new technical solution is proposed in the present application. SUMMARY

[0004] In order to improve the success rate of nuciferine animal experiments, the present application provides an experimental mouse feeding control system applied to nuciferine research.

[0005] The present application provides an experimental mouse feeding control system applied to nuciferine research, which adopts the following technical solution:

[0006] An experimental mouse feeding control system applied to nuciferine research, comprising a control module, and an indicator light, a detection mechanism and a display electrically connected to the control module, the detection mechanism is installed in a feeding cage and is used for detecting feeding behavior, and comprises a mesh plate and a weighing sensor, a load-bearing table is arranged below the mesh plate, the weighing sensor is installed on the load-bearing table, the mesh plate is located on the weighing sensor, and a feeding trough in the feeding cage is located on the mesh plate, the mesh plate is used for experimental mice to return to the position after taking out; the indicator light is two and is installed in the feeding cage respectively, the indicator light is of RGB lamp type, and the control module is configured to:

[0007] Define a weight interval M1 caused by feeding to change weight, and define a weight interval M2 caused by injection to change weight;

[0008] Define one indicator light as a feeding indicator light and switch between color A and color C, and define the other indicator light as an injection indicator light and switch between color B and color D;

[0009] If the weight data change received by the weighing sensor is greater than G1 and falls within the weight interval M1, the feeding indicator light is switched to color A; wherein G1 is the minimum value of the weight change when the experimental mouse is fed;

[0010] If the weight data change received by the weighing sensor is greater than G2 and falls within the weight interval M2, the injection indicator light is switched to color B; wherein G2 is the minimum value of the weight change when the experimental mouse is injected, G2>G1;

[0011] Timing is performed, and if the feeding indicator light is not switched to color A after timing for t1 length, the feeding indicator light is switched to color C; wherein t1 length is the interval length of feeding the experimental mouse;

[0012] If the injection indicator light is not switched to color B after timing for t2 length, the injection indicator light is switched to color D; wherein t2 length is the interval length of injecting the experimental mouse;

[0013] If the feeding indicator light or the injection indicator light is switched to color A or color B, the feeding indicator light or the injection indicator light is turned off and timing is restarted.

[0014] Optionally, the top of the breeding cage is rotationally connected with a baffle, the initial state of the baffle is vertically downward, the side wall of the baffle is close to one side of the mesh plate when the baffle is in the initial state, and the distance between the baffle and the mesh plate is at least for the experimental mouse to pass through, and the baffle in the initial state separates the breeding cage into an experimental mouse diet area and an experimental mouse living area; a reduction motor and an induction unit are installed at the top of the side wall of the breeding cage, the rotation shaft of the reduction motor is fixed with the baffle, and the reduction motor and the induction unit are electrically connected to a control module, and the control module is configured to:

[0015] If a signal feedback that the induction unit detects an obstacle is received, the reduction motor is controlled to rotate the baffle after t3 length;

[0016] If a signal feedback that the injection indicator light is switched to color B is received, the reduction motor is controlled to perform a baffle recovery work.

[0017] Optionally, the control module is configured to:

[0018] If a signal feedback that the feeding indicator light is switched to color A is received, the weight data and the specific time currently received by the weighing sensor are recorded, a database is established, and the weight data is uploaded by binding the ID of the weighing sensor;

[0019] According to the ID of the weighing sensor, the experimental mouse information corresponding to the weight data is identified;

[0020] The weight data of any experimental mouse is sorted according to the time axis;

[0021] Based on the weight data and time sequence, a weight history development trend chart of the experimental mouse is generated.

[0022] Optionally, if the cages are multiple and arranged in an array, one control module is arranged in each cage, group string mechanisms are arranged between the control modules for mutual connection, the group string mechanism comprises multiple sliding connection units, the sliding connection unit comprises a protruding block, a groove piece and an electrode sheet, the protruding block is two and is fixed to one side wall and the bottom of the cage respectively, the groove piece is two and is fixed to the side wall and the upper part of the cage opposite to the protruding block, the electrode sheet is four and is installed on the protruding block and the groove piece respectively, the four electrode sheets on the cage are connected to four I / O ends of the control module respectively, the protruding block and the groove piece are in sliding connection, and the electrode sheets of the protruding block and the groove piece connected to each other abut against each other.

[0023] The control module of one cage in the outermost group is selected as a master control unit, and the control modules of the other cages are selected as slave units, the master control unit selects one I / O end of the connected electrode sheet as an initial function end to sequentially initiate the slave interface setting process, the slave statistical process and the address allocation process and make the slave cooperate.

[0024] Optionally, the slave interface setting process comprises:

[0025] The master control unit is configured to:

[0026] select one I / O end of the connected electrode sheet as an initial function end, and send an interface setting instruction through the initial function end;

[0027] if an interface setting instruction returned by a certain slave unit is received, stop;

[0028] The slave unit is configured to:

[0029] if an interface setting instruction sent by the master control unit is received, feed back a signal receiving instruction to the master control unit, and set the receiving I / O end as a signal receiving end;

[0030] select the I / O end corresponding to the other electrode sheet opposite to the electrode sheet position corresponding to the signal receiving end as a signal sending end, and send an interface setting instruction;

[0031] if the sending is successful, the I / O end sending the interface setting instruction is set as the signal sending end;

[0032] if the sending fails, select the I / O end corresponding to the other electrode sheet below the electrode sheet position corresponding to the signal receiving end as the signal sending end, and send an interface setting instruction.

[0033] Optionally, the slave statistical process comprises:

[0034] The master control unit is configured to:

[0035] send the slave statistical data through the initial function end; wherein the slave statistical data comprises a command and a data field, and the initial data field is 0;

[0036] if the slave statistical data returned by a certain slave unit is received, stop;

[0037] The slave unit is configured to:

[0038] if the slave statistical data sent by the master control unit is received, update the slave statistical data, and the updating rule is data field + 1;

[0039] forward the updated slave statistical data to the slave unit of the next order.

[0040] Optionally, the address allocation process comprises:

[0041] The master control unit is configured to:

[0042] read the number N of slave units from the data field in the slave statistical data;

[0043] generate N address combinations according to a preset address generation rule, and obtain a new data field;

[0044] send the address allocation data through the initial function end; wherein the address allocation data comprises an allocation command and the new data field.

[0045] Optionally, the control module is configured to:

[0046] based on the connection order of the breeding cages, divide the multiple breeding cages in the same row into the same group, and allocate a group code;

[0047] based on the group information of the experimental mice input by the experimental personnel, respectively name the group names of each group, match and bind the corresponding group codes, and upload to the database;

[0048] if a feedback signal of switching of any one indicator light is received, search the database to obtain the remaining breeding cages in the group to which the breeding cage of the indicator light belongs, and control the indicator light of the remaining breeding cage to perform flickering prompt work.

[0049] Optionally, the top of the groove piece is provided with a protective sheet, one end of the protective sheet is a slope structure, both ends of the protruding block are wedge-shaped structures that fit the slope structure of the protective sheet, a mounting groove is arranged in the groove piece for the protective sheet to extend and retract, a spring is fixed to the groove wall of the mounting groove, the spring extends horizontally and one end of the spring is fixed to the protective sheet, and when the spring is in a natural state, the protective sheet extends to block the slot of the groove piece.

[0050] Optionally, the control module is configured to:

[0051] Upload and record the experimental mouse feeding / injection schedule input by the experimenter;

[0052] Based on the experimental mouse feeding / injection schedule, determine the working time period triggering the color change of the feeding / injection indicator light, and if the current time is outside the feeding / injection working time, control the corresponding indicator light to always remain off.

[0053] In summary, the present application has the following beneficial technical effects: By setting the indicator light to prompt the feeding or injection behavior, the experimenter can observe the light color of the indicator light to know whether to feed or inject the experimental mouse, which facilitates the experimenter to verify whether the work is missed, effectively reduces the possibility of the experimenter missing feeding or injecting the experimental mouse, and improves the survival rate of feeding experimental mice and the success rate of nuciferine animal experiments. BRIEF DESCRIPTION OF DRAWINGS

[0054] Figure 1 is a control structure connection schematic diagram of the present application;

[0055] Figure 2 is a schematic diagram of the overall structure of the feeding cage of the present application;

[0056] Figure 3 is a sectional view of the feeding cage of the present application;

[0057] Figure 4 is Figure 3 is an enlarged schematic diagram of part A of

[0058] Marked: 1, control module; 2, indicator light; 3, detection mechanism; 31, weighing sensor; 32, mesh plate; 33, load-bearing table; 4, sliding connection unit; 41, recessed part; 42, protruding block; 43, protective sheet; 44, spring; 5, display; 6, baffle; 7, speed reducer motor; 8, induction unit. DETAILED DESCRIPTION

[0059] The following will be described in detail in combination with the accompanying Figures 1-4 The present application will be further described in detail.

[0060] The present application discloses an experimental mouse feeding control system applied to nuciferine research.

[0061] Reference Figure 1The application is applied to the experimental mouse feeding control system for nuciferine research, which comprises a control module 1 and indicator lights 2, a detection mechanism 3 and a display 5 electrically connected to the control module 1, wherein the display 5 is used for displaying a working state or inputting a control instruction by an experimental personnel and can be a computer display screen in a laboratory. The detection mechanism 3 is installed in a feeding cage and is used for detecting feeding behaviors (such as injection and feeding), and comprises a mesh plate 32 and a weighing sensor 31, a load-bearing table 33 is arranged below the mesh plate 32, the weighing sensor 31 is installed on the load-bearing table 33, the mesh plate 32 falls above the weighing sensor 31, that is, the mesh plate 32 is a weighing detection area of the experimental mouse, and the experimental mouse after injection is put back to the mesh plate 32, and the sensing end of the weighing sensor 31 faces the direction of the mesh plate 32.

[0062] Referring to Figure 2 The indicator lights 2 are two in total and are installed on the feeding cage, can be installed on a more conspicuous position of the feeding cage (such as the front surface and a position close to the side edge, without affecting the observation of the state of the experimental mouse), and are RGB light types, can switch multiple colors through a light control unit of the RGB light, and the control module 1 is configured as:

[0063] S1, a weight interval M1 caused by feeding is defined, and a weight interval M2 caused by injection is defined. M1 is estimated according to the total weight of the food and drinking water for the experimental mouse, such as 5-12 g; since the experimental mouse needs to be caught out and put back when being injected, the position for putting back is arranged in the area where the mesh plate 32 is located, and M2 is estimated according to the weight of the experimental mouse itself, such as 18-40 g, and if it is a young mouse, the weight interval can be reduced; the specific interval of M1 and M2 is defined by the experimental personnel according to the state of the experimental mouse.

[0064] S2, one indicator light 2 is defined as a feeding indicator light and is switched between color A and color C, and the other indicator light 2 is defined as an injection indicator light and is switched between color B and color D; for example, color A is green, color C is red, color B is blue, and color D is yellow.

[0065] S3, if the weight data change fed back by the weighing sensor 31 is greater than G1 and falls within the weight interval M1, the indicator light 2 is switched to color A; wherein G1 is a preset minimum weight change value when the experimental mouse is fed. For example, the weight change fed back by the weighing sensor 31 is 7 g, and G1 is the minimum weight change value, that is, 5 g, so it can be judged that the experimental mouse is fed, and the feeding indicator light is switched to green to prompt the experimental personnel that the experimental mouse has been fed.

[0066] If the weight data change fed back by the weighing sensor 31 is greater than G2 and falls in the weight interval M2, the injection indicator light is switched to color B, wherein G2 is a preset minimum weight change value during injection of the experimental mouse, and G2>G1; for example, if the weight change fed back by the weighing sensor 31 is 20 g and G2 is the minimum weight change value during injection, i.e. 18 g, it can be judged that the experimental mouse is injected, and the injection indicator light is switched to blue to prompt the experimental personnel that the injection has been performed.

[0067] S4, timing is performed; if the feeding indicator light is not switched to color A after timing for t1 duration, the feeding indicator light is controlled to be switched to color C; wherein t1 duration is an interval duration of feeding of the experimental mouse, for example, 12 h; if the color of the feeding indicator light is not switched after 12 h, it is judged that the feeding is forgotten, and the color of the feeding indicator light is switched to red to prompt the experimental personnel.

[0068] If the injection indicator light is not switched to color B after timing for t2 duration, the injection indicator light is controlled to be switched to color D; wherein t2 duration is an interval duration of injection of the experimental mouse, for example, 48 h; if the color of the injection indicator light is not switched after 48 h, it is judged that the injection is forgotten, and the color of the injection indicator light is switched to yellow to prompt the experimental personnel.

[0069] S5, if the feeding indicator light or the injection indicator light is switched to color A or color B, the feeding indicator light or the injection indicator light is turned off and timing is restarted; wherein the feeding indicator light or the injection indicator light can be turned off one hour after being switched to color A or color B, which plays a prompting role and is automatically turned off after the experimental personnel completes the injection / feeding work.

[0070] According to the above setting, the feeding / injection behavior is prompted by the indicator light 2, the indicator light 2 switches the light color, which facilitates the experimental personnel to check the light color to verify whether the work is omitted after feeding / injection, effectively reduces the possibility of omission of the experimental personnel in feeding / injection of the experimental mouse, and improves the survival rate of the experimental mouse and the success rate of the animal experiment of theophylline.

[0071] According to the above setting, the misjudgment is reduced, and the indicator light 2 does not need to be kept standby outside the feeding or injection time.

[0072] Reference Figure 2 and Figure 3The top of the cage can be partially opened, and the open part is provided with a cover for easy grabbing of the experimental mouse for injection. After injection, the top cover is put back on. Alternatively, the top of the cage can be opened for grabbing the experimental mouse. The cage can be made of transparent acrylic plate material for easy observation of the inside of the cage. The cage is divided into two parts. The area occupied by the mesh plate 32 is the experimental mouse feeding area, and the area outside the mesh plate 32 is the experimental mouse living area. When feeding the experimental mouse, food and water are placed in the feeding trough in the feeding area for feeding. The experimental mouse living area is covered with sawdust. The height of the experimental mouse feeding area is greater than the height of the experimental mouse living area, effectively preventing the sawdust in the experimental mouse living area from flying into the experimental mouse feeding area with the movement of the experimental mouse, reducing the interference of the sawdust with the weighing sensor 31. The experimental mouse living area is provided with a lifting plate, and the lifting plate is provided with holes for laying sawdust and other materials for feeding the experimental mouse. Through this arrangement, the experimental mouse can excrete urine and other secretions through the holes. The bottom of the cage can be detachable or a chute opening can be provided at the bottom of the cage, and a waste tray can be placed. When cleaning the cage, the bottom of the cage or the chute opening can be removed to take out the waste tray. Lifting the bottom of the experimental mouse living area is conducive to cleaning the experimental mouse living area, maintaining the health of the experimental mouse, and reducing the probability of disease, thereby reducing the probability of experimental failure.

[0073] The top of the cage is rotatably connected to a baffle 6. The initial state of the baffle 6 is vertically downward. When the baffle 6 is vertically downward, it separates the experimental mouse living area and the experimental mouse feeding area. The edge of the baffle 6 is close to one side of the mesh plate 32 but does not touch it, and the distance between the baffle 6 and the mesh plate 32 is at least sufficient for the experimental mouse to pass through. The side of the baffle 6 facing the experimental mouse feeding area can be provided with a limiting block to reduce the possibility of mechanical failure caused by the experimental mouse hitting the baffle 6. A reduction motor 7 and a sensing unit 8 are installed at the top of the side wall of the cage. The sensing unit 8 can be an infrared reflection sensor for detecting whether there is an obstacle (i.e. whether a hand is inserted to catch the experimental mouse) blocking the infrared light beam. The infrared reflection sensor is installed at the top of the open side wall. A multi-beam infrared reflection sensor can be used to improve the accuracy of detection. If the top of the cage is detachable, the infrared reflection sensor can be installed at the top of the side wall of the cage. The reduction motor 7 is fixed to the rotating shaft of the baffle 6 through a shaft coupling. The emission end and the receiving end of the sensing unit 8 are horizontally emitted and received, and are oppositely distributed at the top of the side wall.

[0074] The reduction motor 7 and the sensing unit 8 are electrically connected to a control module 1. The control module 1 is configured as follows:

[0075] If the signal feedback that the sensing unit 8 detects the obstacle is received, the control module 1 controls the reduction gear motor 7 to execute the rotating baffle 6 work after the t3 time length; wherein the t3 time length is the preset time for taking out the experimental mouse, but needs to be less than the time for injecting the experimental mouse, such as 1 minute.

[0076] If the signal feedback that the injection indicator light switches to color B is received, the control module 1 controls the reduction gear motor 7 to execute the baffle 6 recovery work. The injection indicator light switching to color B indicates that the injection has been completed and the experimental mouse is put back, so the baffle 6 can be restored to the vertical state.

[0077] According to the above setting, the experimental personnel take out the experimental mouse from the experimental mouse living area, and after the sensing unit 8 detects the taking action, the reduction gear motor 7 drives the baffle 6 to rotate and block the top opening of the experimental mouse living area, so that the experimental personnel cannot put the experimental mouse back into the experimental mouse living area, effectively preventing the experimental personnel from mistakenly putting the experimental mouse back into the experimental mouse living area after injecting the experimental mouse, so as to improve the accuracy of injection behavior detection.

[0078] In another embodiment of the present application, the control module 1 is configured to:

[0079] Upload and record the experimental mouse feeding / injection schedule input by the experimental personnel; for example, feeding needs to be performed from 8:00 to 9:00 in the morning and from 8:00 to 9:00 in the evening every day, and injection needs to be performed from 8:00 to 9:00 every other day.

[0080] Based on the experimental mouse feeding / injection schedule, determine the working time period for triggering the color change of the feeding / injection indicator light, and if the current time is outside the feeding / injection working time, control the corresponding indicator light 2 to always remain off. For example, according to the above schedule, compare whether the current time is within the feeding or injection time, and if it is outside the feeding or injection time, always keep the indicator light 2 off, even if the weight data change feedback by the weighing sensor 31 reaches the preset requirement, the indicator light 2 is always off.

[0081] Further, in another embodiment of the present application, a vibration motor can be selected to be arranged below the mesh plate 32, the vibration motor is installed in a preset mounting seat of the breeding cage, and the vibration motor and the mesh plate 32 are in contact and used to vibrate the mesh plate 32, so that the wood chips and the like that may be carried by the experimental mouse to the mesh plate 32 can be cleaned by vibration, thereby reducing the probability of detection error. The vibration motor vibrates outside the feeding / injection working time, which can be three times a day, each for 30s for cleaning work.

[0082] Since the experimental mouse may die halfway during the breeding process before the experimental results are detected, the data for experimental comparison is reduced, and the following settings are made:

[0083] The control module 1 is configured to:

[0084] If the feeding indicator light switches to color A, record the weight data fed back by the current weighing sensor 31 and the specific time, establish a database and upload the weight data bound to the ID of the weighing sensor; if the weight of the experimental mouse is detected immediately after adding food, the weight of the newly added food is subtracted.

[0085] According to the ID of the weighing sensor, the weight data corresponding to the experimental mouse information is identified; the identification example is that the user preloads the ID of the weighing sensor and the number of the experimental mouse, and they are one-to-one corresponding.

[0086] The weight data of any experimental mouse is sorted according to the time axis, and the experimental mouse weight history development trend chart is generated based on the weight data and time sequence. By recording the weight data of the experimental mouse at each meal, and generating the weight data into a historical development trend chart (such as a line chart) according to the time sequence, the weight development trend of the experimental mouse can be more intuitively seen, even if the experimental mouse dies in the middle, the data can be used as a reference.

[0087] According to the above setting, the data utilization rate of the experimental mouse is improved, even if the experimental mouse dies in the middle, the feeding / injection time cost will not be wasted, and the weight data recorded during feeding can be used as a reference.

[0088] If the breeding cage is multiple and the multiple breeding cages are arranged in an array, the array direction is a vertical array, and a shelf can be provided for the breeding cage to be placed; each breeding cage is provided with a control module 1, and a group string mechanism is arranged between the control modules 1, the group string mechanism is used to connect adjacent breeding cages to each other, and the group string mechanism includes a plurality of sliding connection units 4, the sliding connection unit 4 includes a protruding block 42, a groove piece 41 and an electrode sheet. Among them, the protruding block 42 is two and is fixed on one side wall and the bottom of the breeding cage, the groove piece 41 is two and is fixed on the other side wall and the top of the breeding cage opposite to the protruding block 42, and the electrode sheet is installed on the protruding block 42 and the groove piece 41. Thus, there are four electrode sheets on one breeding cage, and the four electrode sheets are respectively electrically connected to the four I / O ports of the control module 1, the protruding block 42 and the groove piece 41 are slidingly connected, and the electrode sheets of the protruding block 42 and the groove piece 41 inserted with each other abut each other, and the I / O ports of each control module 1 are connected through the electrode sheets. Assuming that a shelf is provided for the breeding cage to be placed, a sliding groove is needed to be provided on the shelf for the protruding block 42 of the breeding cage to slide, so as not to hinder the connection of the breeding cages to each other.

[0089] The top of the recessed part 41 is provided with a protective sheet 43, one end of the protective sheet 43 is a bevel structure, both ends of the protrusion 42 are wedge structures matched with the angle of the bevel structure of the protective sheet 43, a mounting groove is arranged in the recessed part 41 for the protective sheet 43 to extend and retract, a spring 44 is fixed in the mounting groove, one end of the spring 44 is connected to the side wall of the protective sheet 43, the extension direction of the spring 44 is transverse, and when the spring 44 is in the initial state, the protective sheet 43 extends out of the mounting groove and blocks the slot of the recessed part 41. According to the above arrangement, when the protrusion 42 slides and inserts, the protective sheet 43 can be pushed open, and when the protrusion 42 slides and pulls out, the protective sheet 43 can automatically restore to block the slot of the recessed part 41 by the action force of the spring 44. The protective sheet 43 can be provided with two sheets, which are arranged in an up-down staggered manner and installed on the two side groove walls of the recessed part 41. When the two protective sheets 43 extend, the protective sheets 43 overlap each other, which can better block the slot of the recessed part 41.

[0090] The control module 1 of one of the breeding cages in the outermost group is selected as the master control unit (such as the breeding cage in the upper right corner), and the control modules 1 of the other breeding cages are slaves. Signals or instructions are transmitted through the electrode sheets abutting each other. The master control unit selects an I / O end of a connected electrode sheet as an initial function end to sequentially initiate the slave interface setting process, the slave statistical process and the address allocation process and make the slaves cooperate.

[0091] According to the above arrangement, the number of breeding cages can be automatically counted, and the addresses can be allocated according to the physical connection order, which is more convenient and efficient.

[0092] The slave interface setting process includes:

[0093] The master control unit is configured to:

[0094] An I / O end of a connected electrode sheet is selected as an initial function end, and an interface setting instruction is sent through the initial function end; for example, the I / O end of the electrode sheet connected between the breeding cage in the upper right corner and the breeding cage on the side is selected as the initial function end, and an interface setting instruction is sent to the slave unit through the I / O end. The sending order is based on the physical connection order and can be from right to left or from top to bottom.

[0095] If an interface setting instruction returned by a certain slave unit is received, the process is stopped. If an interface setting instruction returned by a certain slave unit is received, it means that the interface setting of each slave unit has been completed.

[0096] The slave unit is configured to:

[0097] If the interface setting instruction sent by the master unit is received, a signal receiving instruction is fed back to the master unit, and the I / O end receiving the signal is set as a signal receiving end; for example, when the feeding cage is placed in the normal direction, the I / O end connected to the electrode piece on the right side receives the interface setting instruction sent by the master unit, and feeds back a signal receiving instruction to the master unit, so that the I / O end is set as a signal receiving end.

[0098] The I / O end corresponding to the electrode piece opposite to the electrode piece corresponding to the signal receiving end is selected as a signal sending end, and an interface setting instruction is sent; for example, when the feeding cage is placed in the normal direction, the I / O end connected to the electrode piece on the left side is selected as a signal sending end, and an interface setting instruction is sent to the next slave unit.

[0099] If the sending is successful, the I / O end sending the interface setting instruction is set as a signal sending end; the sending success indicates that the signal receiving instruction fed back by the next slave unit is received.

[0100] If the sending fails, the I / O end corresponding to the electrode piece below the electrode piece corresponding to the signal receiving end is selected as a signal sending end, and an interface setting instruction is sent; the sending failure indicates that the signal receiving instruction fed back by the next slave unit is not received, so that the I / O end corresponding to another electrode piece is selected as a signal sending end; the sending sequence of the application is from right to left and from top to bottom, so that the I / O end corresponding to the electrode piece below the electrode piece when the feeding cage is placed in the normal direction is selected as a new signal sending end to resend the interface setting instruction.

[0101] According to the above setting, the signal sending end and the signal receiving end of each control module 1 can be automatically confirmed, and subsequent slave statistical process and address allocation process can be facilitated.

[0102] The slave statistical process includes:

[0103] The master unit is configured to:

[0104] The slave statistical data is sent through the initial function end; the slave statistical data includes an instruction and a data field, and the initial data field is 0; the instruction is a statistical instruction, and each slave is required to perform statistical work, and the data field is used to record the number of all slaves.

[0105] If the slave statistical data returned by a certain slave unit is received, the process is stopped; for example, there is no other slave unit that has not performed statistical work on the side of the slave unit at the lower right corner, the statistical data is transmitted back, and the master unit receives the statistical data to complete the statistics and stop working.

[0106] The slave unit is configured to:

[0107] If the slave statistical data sent by the master control unit is received, the slave statistical data is updated, and the updating rule is data field + 1; for example, the slave unit on the side of the master control unit receives the statistical instruction, and the original data field 0 is added by 1;

[0108] The updated slave statistical data is forwarded to the next order slave unit; after the slave unit completes its own + 1 counting, the statistical instruction is passed to the next adjacent slave unit, for example, the current slave unit completes the statistical work, the updated data field is passed, and the next slave unit adds 1 to the updated data field to represent the current number of slave units; according to the above setting, the total number of breeding cages can be automatically counted, and the statistical efficiency is improved.

[0109] The address allocation process includes:

[0110] The master control unit is configured to:

[0111] The number of slave units N is read from the data field in the slave statistical data; for example, the data returned by the last slave unit to the host is 11, and the number of slave units is 11.

[0112] N address combinations are generated according to the preset address generation rule to obtain a new data field; the address generation rule can be to encode the slave units according to their order, and the size of the encoding increases according to the number of slave units; for example, 1, 2, 3, ….

[0113] The address allocation data is sent through the initial function end; the address allocation data includes allocation instructions and a new data field. The master control unit sends the address allocation instructions and the new data field, and the new data field includes multiple addresses, so that the slave units receive their own addresses in turn, and the slave units forward the remaining addresses to the next order slave unit after receiving their own addresses, until the address allocation is completed.

[0114] According to the above setting, the addresses can be allocated in order, the slave units receive their own addresses in turn, and the encoding is automatically completed, improving the work efficiency.

[0115] The control module 1 is configured to:

[0116] Based on the connection order of the breeding cages, the multiple breeding cages in the same row are divided into the same group, and a group code is allocated; for example, the breeding cages are arranged in a 4*4 array, the control module 1 of the breeding cages in the first row is allocated with codes 0-3, and a group code A is added, the control module 1 of the breeding cages in the second row is allocated with codes 4-7, and a group code B is added, and so on.

[0117] Based on the group information of the experimental mice input by the experimenters, the group name of each group is named, the corresponding group code is matched and bound, and is uploaded to the database; for example: the first row is the HFD control group, and the name is bound with the group code A; the second row is the low-dose lotus leaf alkaloid group, and the name is bound with the group code B, and so on; by adding the group name, it is convenient to distinguish the experimental mice of different groups, and if the different groups have different feeding methods, it is convenient to input different feeding / injection times and match the groups for binding.

[0118] If any one of the feedback signals of the indicator light 2 switching is received, the database is searched to obtain the remaining cages in the group to which the cage belongs, and the indicator light 2 of the remaining cages is controlled to perform flickering prompting work. For example: the feeding indicator light of one cage in the group code A starts to switch, it is judged that the experimenter starts to feed, then the database is searched to obtain the remaining cages in the group, the feeding indicator light is controlled to flicker, prompting the experimenter to feed, until the experimenter feeds, the indicator light 2 is always on with a preset color.

[0119] Through the above setting, the behavior of feeding the experimental mice can be monitored and prompted in groups, and the indicator light 2 flickers to play a prompting role, reducing the probability of the experimenter missing feeding / injection.

[0120] The above are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application, therefore: any equivalent changes made on the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A laboratory mouse feeding and control system for lotus leaf alkaloid research, characterized in that: The system includes a control module (1) and indicator lights (2), a detection mechanism (3), and a display (5) electrically connected to the control module (1). The detection mechanism (3) is installed in the feeding cage and is used to detect feeding behavior. It includes a mesh plate (32) and a weighing sensor (31). A support platform (33) is set below the mesh plate (32). The weighing sensor (31) is installed on the support platform (33). The mesh plate (32) is located on the weighing sensor (31). The feed trough in the feeding cage is located on the mesh plate (32). The mesh plate (32) is the position where the experimental mouse is put back after being taken out. There are two indicator lights (2) installed in the feeding cage respectively. The indicator lights (2) are RGB lights. The control module (1) is configured as follows: Define the weight range M1 for weight change caused by feeding, and define the weight range M2 for weight change caused by injection; Define one indicator light (2) as a feeding indicator light and switch between color A and color C, and define another indicator light (2) as an injection indicator light and switch between color B and color D; If the weight data received from the weighing sensor (31) is greater than G1 and falls within the weight range M1, then the feeding indicator light is switched to color A; where G1 is the preset minimum weight change when feeding the experimental mouse. If the weight data received from the weighing sensor (31) is greater than G2 and falls within the weight range M2, then the injection indicator light is switched to color B. Here, G2 is the preset minimum weight change when injecting the experimental mouse, and G2 > G1. The timing is set, and if the feeding indicator light does not switch to color A after the timing reaches t1, the feeding indicator light is controlled to switch to color C; where t1 is the interval between feedings of the experimental mice. If the injection indicator light does not switch to color B after the timer reaches t2, then the injection indicator light will be switched to color D; where t2 is the interval between injections in the experimental mouse. If the feeding indicator or injection indicator switches to color A or color B, then turn off the feeding indicator or injection indicator and restart the timer.

2. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 1, characterized in that: A baffle (6) is rotatably connected to the top of the breeding cage. The baffle (6) is initially vertically downward. When the baffle (6) is in its initial state, its sidewall is close to one side of the mesh plate (32), and the distance between the baffle (6) and the mesh plate (32) is at least enough for the experimental mouse to pass through. The baffle (6) in its initial state separates the breeding cage into a feeding area and a living area for the experimental mouse. A geared motor (7) and a sensing unit (8) are installed on the top of the sidewall of the breeding cage. The rotation shafts of the geared motor (7) and the baffle (6) are fixed. The geared motor (7) and the sensing unit (8) are electrically connected to the control module (1). The control module (1) is configured as follows: If the sensor unit (8) receives a signal feedback that it has detected an obstacle, then after time t3, the speed reduction motor (7) is controlled to rotate the baffle (6). If a signal feedback is received that the injection indicator light has switched to color B, the geared motor (7) is controlled to perform the baffle (6) restoration work.

3. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 2, characterized in that, The control module (1) is configured as follows: If a signal feedback is received that the feeding indicator light has switched to color A, the weight data fed back by the current weighing sensor (31) and the specific time are recorded, a database is established, and the weight data is bound to the ID of the weighing sensor and uploaded. The experimental mouse information corresponding to the weight data is identified based on the ID of the weighing sensor; The weight data of any single laboratory mouse were sorted according to the time axis; A historical trend chart of mouse weight was generated based on weight data and time sequence.

4. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 1, characterized in that: If there are multiple breeding cages arranged in an array, each breeding cage is equipped with a control module (1). The control modules (1) are connected to each other by a stringing mechanism. The stringing mechanism includes multiple sliding connection units (4). Each sliding connection unit (4) includes a protrusion (42), a groove (41), and an electrode plate. There are two protrusions (42) and they are respectively fixed to one side wall and the bottom of the breeding cage. There are two grooves (41) and they are fixed to the opposite side wall and the top of the breeding cage and the protrusion (42). There are four electrode plates and they are respectively installed on the protrusion (42) and the groove (41). The four electrode plates on the breeding cage are respectively connected to the four I / O terminals of the control module (1). The protrusion (42) and the groove (41) are slidably connected, and the electrode plates of the protrusion (42) and the groove (41) connected to each other abut against each other. The control module (1) of one of the breeding cages located on the outermost side is selected as the master control unit, and the control modules (1) of the other breeding cages are slave units. The master control unit selects an I / O terminal connected to the electrode plate as the initial functional terminal and sequentially initiates the slave interface setting process, slave statistics process and address allocation process and commands the slave to cooperate.

5. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 4, characterized in that, The slave interface setup process includes: The main control unit is configured as follows: Select an I / O terminal connected to the electrode plate as the initial function terminal, and send the interface setting command through the initial function terminal; If an interface setting command is received from a slave unit, then stop; The slave unit is configured as follows: If an interface setting command is received from the main control unit, a signal receiving command is sent back to the main control unit, and the receiving I / O terminal is set as a signal receiving terminal. Select the I / O terminal corresponding to the electrode plate opposite the position of the signal receiving terminal as the signal transmitting terminal, and set the transmitting interface command. If the transmission is successful, the I / O terminal of the sending interface setting command will be used as the signal sending terminal; If transmission fails, the I / O terminal corresponding to another electrode below the electrode position of the signal receiver is selected as the signal transmitter, and the transmission interface setting command is executed.

6. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 4, characterized in that, The slave machine statistics process includes: The main control unit is configured as follows: The slave statistics are sent through the initial function terminal; the slave statistics include instructions and data fields, and the initial data field is 0; If slave statistics data are received from a slave unit, then the process stops. The slave unit is configured as follows: If the slave statistics data is received from the master control unit, the slave statistics data is updated, and the update rule is data field +1; The updated slave statistics data are forwarded to the next slave unit in sequence.

7. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 4, characterized in that, The address allocation process includes: The main control unit is configured as follows: The number of slave units N is obtained by reading the data field from the slave statistics data. N address combinations are generated according to preset address generation rules to obtain a new data field; Address allocation data is sent through the initial function terminal; the address allocation data includes allocation instructions and new data fields.

8. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 7, characterized in that, The control module (1) is configured as follows: Based on the connection order of the rearing cages, multiple rearing cages in the same row are divided into the same group and assigned a group code; Based on the group information of the experimental mice entered by the experimenters, each group was named, the corresponding group code was matched and bound, and then uploaded to the database; If a feedback signal is received indicating that any indicator light (2) has switched, the database is searched to find the remaining cages in the group to which the cage of that indicator light (2) belongs, and the indicator light (2) of the remaining cages is controlled to flash to indicate that the cage is switched.

9. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 4, characterized in that: The top of the groove (41) is provided with a protective plate (43). One end of the protective plate (43) is a bevel structure. Both ends of the protrusion (42) are wedge-shaped structures that fit the bevel structure of the protective plate (43). The groove (41) is provided with a mounting groove for the protective plate (43) to extend and retract. A spring (44) is fixed to the groove wall of the mounting groove. The spring (44) is laterally telescopic and one end of the spring (44) is fixed to the protective plate (43). When the spring (44) is in its natural state, the protective plate (43) extends out and covers the groove opening of the groove (41).

10. The experimental mouse feeding and control system for lotus leaf alkaloid research according to claim 1, characterized in that, The control module (1) is configured as follows: Upload and record the feeding / injection schedule for laboratory mice entered by the experimenters; Based on the feeding / injection schedule of the experimental mice, the working time period for triggering the color change of the feeding / injection indicator light is determined. If the current time is outside the feeding / injection working time, the corresponding indicator light (2) is controlled to always remain off.

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