Application of acer truncatum seed oil as base oil in cosmetics

Abstract

The invention relates to application of acer truncatum oil as base oil in cosmetics, and belongs to the technical field of cosmetics. Experiments show that the acer truncatum oil has the effects of moisturizing, resisting aging and resisting oxidation, and cosmetics with the effects of moisturizing, relieving, resisting inflammation, repairing and the like can be developed by taking the acer truncatum oil as functional base oil, such as body lotion and hand cream for sensitive skin and dry, red and swollen skin in winter; in combination with the beauty trend of whole oil maintenance, a pure oil product / an oil skin-care basic raw material and a water / oil two-phase anti-aging moisturizing and repairing product can be developed. As a natural grease substance, the acer truncatum seed oil has low solubility in a water-based solvent, so that the application of the acer truncatum seed oil in water-based cosmetics is limited. According to the invention, a double-gel system consisting of hydrogel and oleogel is adopted, so that the solubility of the acer truncatum seed oil in a water phase is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the application of Acer truncatum Bunge seed oil as base oil in cosmetics, belonging to the technical field of cosmetics. BACKGROUND

[0002] Base oil is a non-volatile oil extracted from the seeds, flowers, rhizomes or fruits of plants, which can lubricate the skin, can be directly used for skin massage, and is also the best base oil for diluting essential oil; it is also widely used for oil massage care of the skin of newborns and infants, and for the treatment of various skin diseases such as atopic dermatitis, xeroderma, eczema, psoriasis and other skin pathologies. Therefore, base oil plays an important role in the development of health care products and natural and environmentally friendly cosmetics in recent years, and has attracted great attention. The first record of its use can be traced back to China around 200 BC. The base oils currently sold on the market include jojoba oil, sweet almond oil, grape seed oil, rosehip oil, olive oil, argan oil, coconut oil, sunflower seed oil, white kernel oil, etc., but the intellectual property rights thereof are controlled by foreign companies.

[0003] 95% of the components of base oil extracted from plants are polyols, long-chain saturated or unsaturated monocarboxylic acids (oleic acid, linoleic acid and linolenic acid) triglycerides, and 5% are characteristic components in different plant extracts, such as phospholipids, glycolipids, sulfatides, sphingolipids, waxes, squalene, carotenoids and chlorophyll pigments, vitamin E, phytosterols, polyphenols, triterpenols, etc. Among them, triterpenols and the like have antioxidant, anti-inflammatory and other effects. The use of base oil on the skin can enhance the skin barrier function, improve the epidermal temperature regulation function, effectively moisturize, etc.

[0004] The non-specific effects of base oil on the skin are due to its occlusive effect through hydrophobicity, which can coat and lubricate the stratum corneum and the intercellular space of keratinocytes to protect the skin, keep the skin moisturized, reduce trans-epidermal water loss (TEWL), and thus improve the hydration of the skin. The specific effects of base oil are mediated by free fatty acids and non-saponified compounds, including antibacterial, anti-inflammatory and antioxidant effects. In addition, free fatty acids and triglycerides have been shown to penetrate the stratum corneum and enhance the transdermal absorption of other substances. Therefore, the combination of free fatty acids and triglycerides in base oil plays a key role in thickening, emulsifying, coating carriers and carrying in cosmetic formulations, making base oil an indispensable basic ingredient of cosmetics.

[0005] In summary, cosmetic base oil has basic functions such as emulsification, thickening, water retention, etc., and also plays specific effects such as anti-aging, anti-allergy, anti-skin inflammation, etc. Therefore, it is of great significance to develop cosmetic base oil with Chinese independent intellectual property rights by taking advantage of the diversity of plant species in China, as well as the advantages of agricultural and industrial mass production.

[0006] Acer truncatum Bunge is a deciduous tree of Aceraceae, with single leaf, opposite, paper, often five-lobed, widely distributed in China, and is a unique tree species in China. Acer truncatum oil (i.e. Acer truncatum seed oil) is a vegetable oil processed from the seeds of Acer truncatum. Acer truncatum seed oil has been listed as a new food raw material by the state in 2011. Taking this kind of bulk vegetable oil as an effective basic oil raw material to develop a product with higher value, such as cosmetics, will be a new industry road for Acer truncatum seed oil. Acer truncatum seed oil is rich in fatty acids, and also contains flavonoids and polyphenols, vitamins A and E, plant terpenes, sterols and the like, so that it has the effects of anti-inflammatory, soothing, anti-aging and the like, and has the conditions of high-value effective plant oil. SUMMARY

[0007] In view of the deficiencies of the prior art, the application provides an application of Acer truncatum seed oil as a basic oil in cosmetics.

[0008] The technical scheme of the application is as follows: The application of Acer truncatum seed oil as a basic oil in cosmetics.

[0009] According to the application, the Acer truncatum seed oil can promote the expression of type 1 collagen and reduce the expression of MMP9, and has the effect of anti-aging.

[0010] According to the application, the Acer truncatum seed oil has the effects of anti-oxidation and moisturizing.

[0011] According to the application, the Acer truncatum seed oil has the effect of emulsification.

[0012] A cosmetic composition containing Acer truncatum seed oil.

[0013] According to the application, the cosmetic composition further comprises other active ingredients or adjuvants acceptable in the cosmetic field.

[0014] According to the application, the cosmetic composition is a double gel of Acer truncatum seed oil.

[0015] Further preferably, the double gel of Acer truncatum seed oil is a uniform gel system formed by stirring an oil gel and a hydrogel at room temperature.

[0016] Further preferably, the oil gel is composed of Acer truncatum seed oil, an emulsifier and water, wherein the emulsifier is laureth-4, and the mass ratio of the Acer truncatum seed oil, the emulsifier and the water is 3:12:2.

[0017] Further preferably, the preparation method of the oil gel is as follows: the emulsifier and the water are added to the Acer truncatum seed oil, and stirred at 70-80 DEG C until completely dissolved, and then treated at a constant temperature of 0-4 DEG C for 24-30 h to obtain the oil gel.

[0018] Further preferably, the preparation method of the hydrogel is: adding a calcium carbonate solution with a mass concentration of 0.04-0.06% into a sodium alginate solution with a mass concentration of 1-5% after stirring the sodium alginate solution uniformly at 70-80°C, the mass ratio of the sodium alginate solution to the calcium carbonate solution being 2000:(0.9-1.1), and cooling to 20-30°C to form the hydrogel.

[0019] Further preferably, the oil gel has a mass percentage of 60-75% in the double gel.

[0020] The cosmetic composition is applied in cosmetics.

[0021] According to the application, the cosmetic has the effects of moisturizing, soothing, anti-inflammatory, repairing, anti-aging and antioxidant.

[0022] The cosmetic contains the king sago seed oil.

[0023] According to the application, the cosmetic has the effects of moisturizing, soothing, anti-inflammatory, repairing, anti-aging and antioxidant.

[0024] The cosmetic includes skin care products.

[0025] Beneficial effects: The unsaturated fatty acid content of the king sago seed oil is up to 92% or more, which is close to that of rosehip oil and is the second highest among all high-value plant oils. The proportion of unsaturated fatty acids in the king sago seed oil is the closest to the proportion of fatty acids on the human skin among all high-value plant oils. The content of ω-3 and ω-6 unsaturated fatty acids in the king sago seed oil is as high as 50%, which is only inferior to that of avocado oil and rosehip oil, and the king sago seed oil can excellently inhibit inflammatory factors TNF-α and IL-6, and has the effects of anti-inflammation, wound healing promotion and anti-aging. The king sago seed oil contains 5-7% of nervonic acid (ω-9) unsaturated fatty acid, which has the effects of anti-inflammation and protection of the nervous system, and this is a unique effect of the king sago seed oil. The content of vitamin E in the king sago seed oil is about 125 mg / 100 g, which is much higher than that of olive oil, so that the king sago seed oil has strong antioxidant and anti-aging ability and stability. Human efficacy experiments show that the king sago seed oil can reduce the trans-epidermal water loss rate of the skin within 2-24 h, and has an instant repair function, which is rarely seen in other high-value cosmetic plant oils. In summary, the king sago seed oil is a high-value cosmetic base oil.

[0026] The application finds through experiments that the Acer truncatum seed oil has the effects of moisturizing, anti-aging and anti-oxidation, and can develop cosmetics with effects of moisturizing, soothing, anti-inflammation and repairing, such as body lotion and hand cream for sensitive skin and dry and red skin in winter, by taking the Acer truncatum seed oil as "efficacy type base oil". In combination with the "all oil care" beauty trend, the Acer truncatum seed oil can also be used to develop pure oil products, oil-based skin care raw materials and water / oil two-phase anti-aging moisturizing and repairing products. As a natural oil substance, the solubility of the Acer truncatum seed oil in water-based solvents is low, which limits its application in water-based cosmetics. The application adopts a double gel system composed of hydrogel and oil gel to improve the solubility of the Acer truncatum seed oil in water phase. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 LC spectrum of the Acer truncatum seed oil 1 H NMR spectrum.

[0028] Figure 2 LC spectrum of the Acer truncatum seed oil

[0029] Figure 3 GC-MS spectrum of the Acer truncatum seed oil

[0030] Figure 4 Survival rate column chart of HDF cells under the action of Acer truncatum seed oil with different concentrations

[0031] Figure 5 Collagen type I expression of HDF cells under the action of Acer truncatum seed oil, wherein A is an SDS-PAGE gel electrophoresis chart of collagen type I, and B is a column chart of collagen type I expression level.

[0032] Figure 6 Column chart of collagenase expression level of HDF cells under the action of Acer truncatum seed oil

[0033] Figure 7 ROS level detection results of HaCaT cells under the action of Acer truncatum seed oil, wherein the left chart is a ROS flow cell detection chart, and the right chart is a column chart of ROS level statistics results.

[0034] Figure 8 Mixed solution chart of Acer truncatum seed oil and deionized water

[0035] Figure 9 Column chart of NO level of an inflammatory cell model under the action of Acer truncatum seed oil with different concentrations

[0036] Figure 10 Centrifugal test results of Acer truncatum seed oil double gel samples, wherein, from left to right in the chart, BG40, BG50, BG60 and BG75 are arranged.

[0037] Figure 11 The results of the alternating hot and cold cyclic treatment test on the double gel samples of Acer truncatum seed oil are shown in the figure. From left to right, they are BG40, BG50, BG60, and BG75.

[0038] Figure 12 The image shows the infrared spectrum of the double-gel sample of Acer truncatum seed oil.

[0039] Figure 13 The bar chart shows the conductivity of the double-gel sample of Acer truncatum seed oil.

[0040] Figure 14 This is a frequency scan of a double-gel sample of Acer truncatum seed oil containing beeswax.

[0041] Figure 15 A bar chart showing the changes in skin stratum corneum moisture content for different emulsion sample groups.

[0042] Figure 16 The repair effect of different emulsion sample groups on the skin model torn by tape. Detailed Implementation

[0043] The technical solution of the present invention will be further described in detail below with reference to the embodiments. Unless otherwise specified, the experimental steps involved in the embodiments are conventional operations in the art. Unless otherwise specified, the materials and reagents involved in the embodiments are common commercially available products.

[0044] The Acer truncatum seed oil used in this example was purchased from Shandong Ruifeng Agricultural and Forestry Technology Co., Ltd. The relative contents of saturated and unsaturated fatty acids in the Acer truncatum seed oil were tested as follows: Table 1. Relative content of major saturated and unsaturated fatty acids in the Acer truncatum seed oil used in the examples

[0045] Among them, the unsaturated fatty acid content of Acer truncatum seed oil reaches 92.49%, and it contains 5.52% functional nervonic acid ω-9, which exists in brain tissue and can promote the repair and regeneration of damaged nerve tissue; it also contains 11.14% eicosenoic acid ω-3.

[0046] Example 1: Composition and structural analysis of Acer truncatum seed oil Take 100mL of commercially available Acer truncatum seed oil, filter it using silica gel powder and defatted cotton, and obtain a clear, transparent, golden-yellow liquid. Then, proceed with the following steps: 1 H NMR, LC, and GC-MS analyses were performed to obtain its composition and purity.

[0047] NMR experimental method for Acer truncatum seed oil: THF-d8 was used as the solvent to dissolve Acer truncatum seed oil to a concentration of 30 mg / mL. The NMR results of the Acer truncatum seed oil were obtained at 400 MHz.1 H NMR spectrum.

[0048] LC method for Acer truncatum seed oil: Dissolve 1g of Acer truncatum seed oil in 10mL of n-hexane, add excess KOH-methanol solution, and reflux in an 80℃ water bath for 15-30 minutes under nitrogen protection. Methyl esterification of fatty acids is performed using the BF3-methanol method. Add 10mL of n-hexane and 10mL of saturated sodium chloride solution, and shake thoroughly. Collect the upper n-hexane layer and dry it using an anhydrous sodium sulfate column. The dried n-hexane solution is then ready for analysis. Use a reversed-phase C18 column with water-acetonitrile-isopropanol as the mobile phase and perform gradient elution according to the program in Table 2. Inject 10μL of the sample and perform LC detection at 242nm.

[0049] Table 2. Mobile phase gradient program for liquid chromatography of Acer truncatum seed oil

[0050] GC-MS experimental method for Acer truncatum seed oil: Same as LC experiment, Acer truncatum seed oil was first pretreated by esterification, and 10 μL was injected.

[0051] Acer truncatum seed oil 1 H NMR spectrum as shown Figure 1 As shown in the spectrum, the signal at chemical shifts of 0.85–0.89 ppm corresponds to the oxymethyl group (-O-CH3) on vitamin E; the sextet signals at chemical shifts of 1.27 ppm and 1.98–2.06 ppm correspond to the methylene group (-CH2-) of the long-chain alkyl group of unsaturated fatty acids; the signal at chemical shift of 1.56 ppm corresponds to the enylmethyl group (-CH=CH-CH3) on vitamin A; the triplet signal at chemical shift of 2.25 ppm corresponds to the methyl hydrogen on the α-carbon of linolenic acid; the sextet signal at chemical shift of 2.75 ppm corresponds to the methyl hydrogen (-CH3) on vitamin E; the signal at chemical shift of 3.56 ppm corresponds to the tetrahydrofuran solvent peak (THF-d8); the two sextet signals at chemical shifts of 4.08 ppm and 4.28 ppm correspond to the isoprene methyl hydrogen on vitamin E; and the sextet signal at chemical shift of 5.3 ppm corresponds to the enylmethyl hydrogen (-CH=CH-CH3) on linolenic acid and linoleic acid.

[0052] The LC spectrum of Acer truncatum seed oil is as follows: Figure 2 As shown in the spectrum, the peak signal at retention time 3.353 min should be the impurity in Acer truncatum seed oil (possibly free fatty acids, phospholipids, or plant tissue residues), accounting for 7.181% of the peak area; the peak signal at retention time 7.133 min belongs to the main component of Acer truncatum seed oil, accounting for 92.819% of the peak area. Therefore, according to the LC analysis results, the purity of Acer truncatum seed oil is 92.819%.

[0053] The GC-MS spectrum of Acer truncatum seed oil is as follows: Figure 3 As shown in the spectrum, the peak signal at retention time 28.094 min should be linoleic acid ω-6, with a peak area accounting for 36.75%; the peak signal at retention time 32.35 min should be oleic acid ω-9, with a peak area accounting for 25.8%; the peak signal at retention time 30.154 min should be erucic acid ω-9, with a peak area accounting for 11.43%; the peak signal at retention time 34.079 min should be eicosenoic acid ω-3, with a peak area accounting for 11.14%; and the peak signal at retention time 25.876 min should be nervonic acid ω-9, with a peak area accounting for 5.52%.

[0054] Example 2: Biological evaluation of cytotoxicity To evaluate whether Acer truncatum oil has cytotoxicity, HDF cells (Human Dermal Fibroblasts) were treated with different concentrations of Acer truncatum oil, and cell viability was detected by CCK-8 assay after 24 h.

[0055] Experimental methods: HDF cells (Human Dermal Fibroblasts) were digested and counted, then resuspended in culture medium to achieve a cell density of 1 × 10⁶ cells / mL. 5 Cells were added to each well of a 96-well plate with 100 μL of cell suspension. A stock solution of *Acer truncatum* seed oil was prepared to a concentration of 200 mg / mL using DMSO. This stock solution was then diluted with culture medium to concentrations of 100 μg / mL, 200 μg / mL, 400 μg / mL, 800 μg / mL, 1600 μg / mL, and 2000 μg / mL, respectively. After overnight cell adhesion, another 100 μL of the above-mentioned different concentrations of *Acer truncatum* seed oil was added to each well, resulting in final working concentrations of 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, 800 μg / mL, and 1000 μg / mL, with three replicates per group. After incubation for 24 hours, the culture medium in the wells was aspirated, and then 10% CCK-8 solution was added, 100 μL per well. After incubation at 37°C for 1.5 h, the absorbance was measured at 450 nm using a microplate reader, and the cell viability was calculated.

[0056] Cell viability results as follows Figure 4 As shown in the figure, the survival rate of HDF cells treated with different concentrations of Acer truncatum seed oil remained above 90%, indicating that Acer truncatum seed oil has almost no cytotoxic activity. Furthermore, at concentrations of 100-800 μg / mL, 6-8% of fibroblasts proliferated, thus Acer truncatum seed oil also has the effect of promoting wound healing.

[0057] Example 3: Biological activity of Acer truncatum seed oil 1. Promotes collagen expression Collagen is a major component of the extracellular matrix, with type 1 and type 3 collagen being the most abundant. Matrix metalloproteinases (MMPs) are a family of zinc-containing peptide hydrolases that can lead to the degradation of collagen.

[0058] Protein detection experimental method: HDF cells were digested and prepared into 2×10⁻⁶ cells. 5 Cell suspension was seeded at a concentration of 2 mL / well in 6-well plates. A stock solution of 200 mg / mL Acer truncatum seed oil was prepared using DMSO. Cells were cultured overnight. After cell attachment, 4 μL of the Acer truncatum seed oil stock solution was added, and the cells were cultured for another 24 h. Cells were collected, total cellular protein was extracted, and the expression of Collagen I, MMP9, and β-actin was detected by SDS-PAGE gel electrophoresis using specific antibodies.

[0059] Type I collagen detection method: HDF cells were used at a concentration of 2×10⁻⁶. 4 Cells were seeded at a density of 100 μL / well in 96-well plates and cultured overnight for 24 hours. A stock solution of *Acer truncatum* seed oil was prepared with DMSO to a concentration of 200 mg / mL, and then diluted with culture medium to concentrations of 300, 600, 900, 1200, and 1600 μg / mL. After overnight cell attachment, 100 μL of culture medium containing different concentrations of *Acer truncatum* seed oil was added to each well, resulting in final concentrations of 150, 300, 450, 600, and 800 μg / mL, with three replicates for each concentration. A negative control group with blank culture medium and a positive control group with 10 ng / mL TGF-β were also included. Cells were cultured for another 24 hours. ELISA assays were performed according to the Elabscience kit instructions: 100 μL of supernatant was added to a new 96-well plate and incubated at 37°C for 90 min. 100 μL of biotinylated antibody working solution was added to each well and incubated at 37°C for 1 h. After washing three times with washing buffer, add 100 μL of HRP enzyme conjugate working solution to each well, incubate at 37°C for 30 min, and wash five times. Add 90 μL of TMB solution to each well and react for 5-10 min, then add 50 μL of stop solution to terminate the reaction. Measure the absorbance at 450 nm using a microplate reader, and calculate the concentration using a linear regression equation based on the type I collagen standard curve.

[0060] The results are as follows Figure 5 As shown, the results indicate that Acer truncatum seed oil promotes the expression of type I collagen and reduces the expression of MMP9, suggesting that Acer truncatum seed oil can increase the content of type I collagen. At a concentration of 450 μg / mL, Acer truncatum seed oil has a 17% type I collagen proliferation effect.

[0061] Method for detecting collagenase: HDF cells were digested and prepared into 2×10⁻⁶ cells. 5 Cell suspension of 2 mL / well was seeded into 6-well plates and cultured overnight. After cell attachment, 55.6 μL of Acer truncatum seed oil was added to a concentration of 25 mg / mL. An equal volume of blank culture medium was added to the control group. Treatment lasted 24 h. RNA was extracted using a total RNA column extraction kit, and after quantification, 1 μg of RNA from each sample was used for reverse transcription. Real-time quantitative PCR was performed on the cDNA obtained from reverse transcription of different samples using SYBR dye. Specific primers were used to detect the expression of MMP1, MMP3, and MMP9, with GAPDH as an internal control. The expression values ​​of each gene in the control group were set to 1.

[0062] Test results as follows Figure 6 As shown, Acer truncatum seed oil, at a concentration of 25 mg / mL, exhibits inhibitory effects on 74% of type I collagenase (mmp-1), 25% of type III collagenase (mmp-3), and 27% of type IX collagenase (mmp-9).

[0063] 2. Antioxidant activity The ability of Acer truncatum oil to scavenge intracellular H2O2-induced ROS was further evaluated using HaCaT cells (human keratinocytes).

[0064] Experimental methods: HaCaT cells (human keratinocytes) were digested, counted, and the cell density was adjusted to 1×10⁶ cells / mL. 5 HaCaT cells were pretreated with 1 mL of cell suspension in each well of a 12-well plate. Three replicates were prepared for each well: a control group, an H2O2 treatment group, and an H2O2 combined with Acer truncatum seed oil treatment group. After overnight cell adhesion, 55.6 μL of Acer truncatum seed oil was added to each well of the H2O2 combined with Acer truncatum seed oil treatment group to achieve a concentration of 25 mg / mL. Equal volumes of blank culture medium were added to the control and H2O2 treatment groups. HaCaT cells were then pretreated for 2 h with H2O2 at a final concentration of 800 μM for 24 h. Reactive oxygen species (ROS) were detected by flow cytometry after staining with the H2DCFDA probe.

[0065] Flow cytometry analysis results as follows Figure 7 As shown, the results indicate that the ROS level in HaCaT cells significantly increased after H2O2 treatment, while ROS production decreased after Acer truncatum seed oil treatment. Acer truncatum seed oil was able to inhibit 48% of H2O2-induced ROS production, indicating that it has antioxidant effects.

[0066] 3. Moisturizing properties According to the detection method reported in the literature, at room temperature, accurately weigh 0.5g of Acer truncatum oil sample and place it in a 50mL centrifuge tube with a diameter of 3cm. Add deionized water equal to 40% of the mass of the Acer truncatum oil sample, with the added water mass being H0. Mix the sample as follows: Figure 8 As shown, after mixing, the centrifuge tubes were placed open in a desiccator containing dry silica gel for 60 hours. The moisture content H of the Acer truncatum oil sample after the 60-hour period was measured. n The moisture retention rate is calculated using the following formula: Moisturizing rate (%) =

[0067] Wherein: H n =0.2011, H0=0.2030. According to the above formula, the moisturizing rate of Acer truncatum oil after 60 hours is 99.1%, indicating that Acer truncatum oil has good moisturizing properties.

[0068] 4. Anti-inflammatory activity Experimental methods: RAW264.7 cells were used at a concentration of 4 × 10⁻⁶ cells / year. 4 Inoculate 100 μL / well of Acer truncatum seed oil into 96-well plates at a density of 100 μL / well. After 24 hours of incubation, discard the culture medium. Prepare a stock solution of Acer truncatum seed oil with DMSO to a concentration of 200 mg / mL, and then dilute the stock solution with culture medium to Acer truncatum seed oil concentrations of 150, 300, 450, 600, and 800 μg / mL, respectively. After discarding the culture medium, add 100 μL of culture medium containing different concentrations of Acer truncatum seed oil to each well, with 3 replicates for each concentration. Set up a negative control with blank culture medium and a positive control with dexamethasone (20 μg / mL), and pretreat for 2 hours. Then add LPS to each well to a final concentration of 1 ng / mL and continue incubation for 24 hours. Perform ELISA detection according to the NO detection kit instructions: take 50 μL of standard and sample into a new 96-well plate, and add 50 μL of Griess Reagent I and 50 μL of Griess Reagent II to each well sequentially. The absorbance was measured at a wavelength of 540 nm using an ELISA reader, and the concentration of NO in the sample was calculated based on the standard curve.

[0069] Test results as follows Figure 9 As shown, Acer truncatum seed oil can reduce NO levels in LPS-induced inflammatory cell models, with concentrated Acer truncatum seed oil at 450 μg / mL exhibiting a 41.8% NO inhibition effect.

[0070] Example 4: Preparation of Acer truncatum seed oil double gel To improve the solubility of Acer truncatum seed oil in aqueous solvents and ensure the smooth progress of subsequent experiments, this invention first prepares Acer truncatum seed oil into a dual-gel system.

[0071] Hydrogel: Prepare a 1% sodium alginate solution, then stir at 500 rpm at 80°C until homogeneous. Add a 0.05% calcium carbonate solution (sodium alginate to calcium carbonate solution mass ratio 2000:1), and cool to 25°C to form a hydrogel. If the sodium alginate solution concentration is low (e.g., 0.5%), gel formation is not possible.

[0072] Oil gel: Emulsifier and water were added to Acer truncatum seed oil and dissolved completely at 80℃ and 500 rpm. The solution was then kept at 4℃ for 24 hours to obtain an oil gel. The amounts of emulsifier, water, and Acer truncatum seed oil are shown in Table 3. Table 3. Amounts of Acer truncatum seed oil, emulsifier, and water in the oleogel system, unit: g

[0073] Therefore, a bigel was finally prepared by adding 12g of lauryl ether-4 and 2g of water to 3g of Acer truncatum seed oil to form a stable oleogel.

[0074] Preparation of bigels: The prepared oleogel and hydrogel were stirred at 500 rpm at room temperature until they were uniformly mixed to form a bigel. Based on the proportion of oleogel in the bigels of 40%, 50%, 60%, and 75%, the bigels were named BG40, BG50, BG60, and BG75, respectively.

[0075] Four double gels, BG40, BG50, BG60, and BG75, were centrifuged at 10000 rpm for 10 min, and the results are as follows: Figure 10 As shown, none of the four bigels exhibited stratification, indicating good stability.

[0076] Four bigels, BG40, BG50, BG60, and BG75, were subjected to alternating hot and cold treatment cycles at room temperature (3 h) and -20°C (3 h), respectively, for a total of 4 cycles. The results are as follows: Figure 11 As shown, none of the four bigels exhibited stratification, indicating good stability.

[0077] Infrared spectroscopy analysis was performed on four bigels: BG40, BG50, BG60, and BG75. The infrared spectra are shown below. Figure 12 As shown, all dual-gel samples were at approximately 3300 cm⁻¹ -1 2900cm -1 1740cm -1 and 1100cm -1Distinct absorption peaks were observed in the vicinity, corresponding to the characteristic vibrations of OH, CH, C=O, and CO bonds, respectively, indicating that the components used were successfully retained and distributed in the samples. With increasing oleogel ratio, the OH absorption peak gradually broadened, indicating that in addition to the hydrogen bonds formed within the hydrogel, hydrogen bonds also formed between the hydrogel and oleogel, suggesting that the bigel structure stabilized the oil-water interface through physical network construction. Furthermore, the main characteristic peaks of each bigel sample were consistent, and no new absorption peaks were observed, indicating that no significant chemical reaction occurred during the preparation of the bigel samples, and the components used were mainly physically mixed.

[0078] To test the emulsifying properties of the bigel sample, a crude emulsion was prepared, and its conductivity was measured. Specifically, a 1% aqueous solution of the bigel was prepared and then mixed with soybean oil at a mass ratio of 4:1. The mixture was homogenized at 10,000 rpm for 2 minutes, and the emulsion conductivity was measured. This emulsion has an O / W structure. The oil-to-hydrogel ratio in the bigel significantly affects the emulsifying properties; the lower the conductivity, the more stable the emulsion. The emulsion conductivity measurement results (…) Figure 13 The results show that BG75 exhibits the best electrical conductivity characteristics, suggesting that it has the best emulsification stability and encapsulation efficiency.

[0079] Furthermore, beeswax, an oleogelizing agent, was added during the preparation of the oleogel, and the performance changes of the bigel were examined. In the oleogel preparation process, 10g of lauryl ether-4, 2g of beeswax (oleogelizing agent), and 2g of water were added to 3g of Acer truncatum seed oil, and the oleogel was prepared according to the above conditions. Based on the oleogel ratio of 50% in the bigel, the bigel was prepared according to the above method. The rheological properties of the bigel were tested, and the frequency sweep results were obtained. Figure 14 As shown, its storage modulus increases sharply with increasing frequency, exhibiting a strong frequency dependence. This indicates that adding an oleogel agent not only does not increase the structural stability of the dual gel of this invention, but also worsens its structural stability. Therefore, lauryl ether-4 can be used alone to prepare oleogels without the need for additional oleogel agents.

[0080] Finally, a bigel aqueous solution was prepared using bigel BG75 for subsequent experiments.

[0081] Example 5: Emulsion containing Acer truncatum seed oil dual gel The emulsion containing Acer truncatum seed oil double gel has the following formula as shown in Table 4.

[0082] Table 4. Emulsion formulations containing Acer truncatum seed oil dual gel, unit: percentage by mass

[0083] Emulsion A5 was prepared based on the above emulsion A1 by adding phase F to the emulsion A1 formulation. The composition and formulation of phase F are shown in the table below: Table 5. Composition and proportion of phase F in emulsion A5 containing Acer truncatum seed oil double gel, unit: mass percentage

[0084] The above-mentioned emulsion containing Acer truncatum seed oil double gel is prepared by the following method: (1) Preparation of phase A and phase B: Weigh phase A and phase B raw materials according to the proportion, heat them at 80°C until completely dissolved, and then cool them to 40°C for later use.

[0085] (2) Preparation of C phase: Weigh the C phase raw materials except gelatin, heat and dissolve them, adjust the pH to 6.0-7.0 with 1 mol / L sodium hydroxide solution, and then add 10g of pre-prepared 5% gelatin solution.

[0086] (3) First, slowly add phase A to the treated phase C and homogenize at a high speed of 5000 rpm for 3 minutes; then add phase B and homogenize again at the same speed of 5000 rpm for 3 minutes to obtain a homogeneous emulsion base. Finally, under a constant temperature of 50°C, add phase D, phase E and phase F (if any) to the emulsion base in sequence and stir until completely dissolved and homogeneous to obtain the final emulsion.

[0087] Emulsion Function Test: Experimental Method: Volunteers cleaned the inner forearms with a cleaning product, rinsed them with water, and then patted them dry with lint-free absorbent paper towels. Laboratory technicians selected six areas on the inner sides of both arms, creating a model of slight skin barrier damage using adhesive tape. Each area was 3cm × 3cm. Test product lotions A1, A2, A3, A4, and A5 were applied evenly to five areas in a single application at a rate of 2.0 g / cm². 2 The other area was left untreated as a blank area. The moisture content of the stratum corneum and the repair of the torn skin in the test area on the inner side of the subject's forearm were tested before injury (BL), 15 min after injury (T#), 10 min after using the test product (T0), 2 h (T2), 4 h (T4), 6 h (T6), and 24 h (T24).

[0088] Compared to 15 minutes after injury, the results of the skin stratum corneum moisture content improvement rate were as follows: Figure 15As shown, in terms of moisturizing, 10 minutes (T0) after application, lotions A1-A4 all effectively increased the moisture content of the stratum corneum, while lotion A5, which contained other functional ingredients, showed the best improvement in stratum corneum moisture content. At 2 and 4 hours after application, lotions A1 and A4 exhibited superior hydrating and moisturizing effects compared to lotions A2 and A3. At 24 hours (T24), lotions A4 and A5 still maintained a high rate of improvement in stratum corneum moisture content. The lotions prepared from Acer truncatum seed oil possess excellent immediate hydrating capabilities, showing significant improvement in stratum corneum moisture content after application. Furthermore, these lotions can be combined with other functional ingredients to create products with even better hydrating effects.

[0089] The repair status of the torn skin is as follows: Figure 16 As shown in the images, after being subjected to tape tearing, the skin exhibited obvious mechanical damage (manifested as stratum corneum peeling and microcrack formation) as well as inflammation and sensitivity. After receiving different sample treatments, the area of ​​redness and inflammation gradually decreased over time, indicating that the specific soothing, anti-inflammatory, and repairing components of Acer truncatum seed oil acted on the skin to reduce the skin irritation and damage caused by tape tearing. Among them, the skin sensitivity of the test area of ​​lotion A5, which contained other functional ingredients, showed the most positive changes, with a significant reduction in redness and inflammation and an improvement in sensitivity.

Claims

1. Application of Acer truncatum seed oil as a base oil in cosmetics.

2. The application as described in claim 1, characterized in that, The Acer truncatum seed oil can promote the expression of type 1 collagen and reduce the expression of MMP9, thus having an anti-aging effect; the Acer truncatum seed oil has antioxidant and moisturizing effects; the Acer truncatum seed oil has emulsifying effects.

3. Cosmetic compositions containing Acer truncatum seed oil.

4. The cosmetic composition according to claim 3, characterized in that, The cosmetic composition may also include other active ingredients or excipients acceptable in the cosmetic field.

5. The cosmetic composition according to claim 3, characterized in that, The cosmetic composition is a double gel of Acer truncatum seed oil.

6. The cosmetic composition according to claim 5, characterized in that, The Acer truncatum seed oil dual gel is a homogeneous gel system formed by stirring an oleogel and a hydrogel at room temperature.

7. The cosmetic composition according to claim 6, characterized in that, The oleogel is composed of Acer truncatum seed oil, emulsifier and water, wherein the emulsifier is lauryl ether-4, and the mass ratio of Acer truncatum seed oil, emulsifier and water is 3:12:

2.

8. The cosmetic composition according to claim 7, characterized in that, The oleogel is prepared by adding emulsifier and water to Acer truncatum seed oil, stirring at 70-80℃ until completely dissolved, and then treating at a constant temperature of 0-4℃ for 24-30 hours to obtain the oleogel.

9. The cosmetic composition according to claim 6, characterized in that, The hydrogel is prepared by stirring a sodium alginate solution with a mass concentration of 1-5% at 70-80°C until homogeneous, then adding a calcium carbonate solution with a mass concentration of 0.04-0.06%, wherein the mass ratio of sodium alginate solution to calcium carbonate solution is 2000:(0.9-1.1), and cooling to 20-30°C to form a hydrogel. Preferably, the oleogel accounts for 60-75% of the mass percentage of the dual gel.

10. The use of the cosmetic composition according to any one of claims 3-9 in cosmetics; Preferably, the cosmetic product has moisturizing, soothing, anti-inflammatory, repairing, anti-aging, and antioxidant effects.

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