Plant acne-removing composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a plant acne-removing composition as well as a preparation method and application thereof. The acne removing composition is prepared from a plant extract and hydroxypropyl cyclodextrin, the plant extract is prepared from the following components in parts by weight: 0.1 to 4 parts of cortex magnoliae officinalis extract, 0.1 to 2 parts of liquorice root extract, 0.1 to 2 parts of peony root bark extract and 0.01 to 0.1 part of radix stephaniae tetrandrae extract. The four extract components are combined according to a specific proportion, the acne removing composition has a remarkable multi-target synergistic effect on the acne removing effect, the pathogenesis of acnes can be comprehensively intervened from multiple links such as bacteriostasis, inflammation resistance, sebum secretion adjustment and barrier repair, the instant and long-acting acne removing effect is achieved, and through inclusion of hydroxypropyl cyclodextrin, the acne removing effect is improved. The dissolvability and bioavailability of the plant extract can be remarkably improved, the stability of the composition is enhanced, the composition is suitable for cosmetics of various systems, transdermal absorption is promoted, and irritation is reduced.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a plant acne-removing composition, a preparation method and application thereof. BACKGROUND

[0002] Acne (acne vulgaris) is a chronic inflammatory disease of the pilosebaceous unit mediated by multiple factors, which is highly prevalent in the areas with dense sebaceous glands such as the face, chest and back. Its pathological process can be divided into four key links: 1, excessive secretion of sebum: androgens (especially dihydrotestosterone DHT) stimulate the proliferation of sebaceous glands, leading to excessive synthesis of lipids (the core reason for the high incidence of adolescent acne); 2, abnormal keratinization of hair follicles: keratinocytes differentiate in disorder, forming microcomedones to block the hair follicle orifice and create an anaerobic environment; 3, microbial colonization and inflammation: Propionibacterium acnes decomposes sebum to generate free fatty acids, activates TLR2 / 4 receptors and releases IL-1α, TNF-α and other pro-inflammatory factors, inducing redness and pustules; 4, secondary barrier damage: inflammatory response damages the skin physical barrier, forming pigmentation and scars (acne marks / acne pits). Factors such as genetics, high GI diet, stress and misuse of cosmetics aggravate acne by regulating the above links.

[0003] Current acne-removing products on the market still have many problems: chemical drugs (such as retinoids, peroxides) can cause dryness and desquamation (> 30% incidence), and long-term use can lead to skin barrier damage; antibiotics (clindamycin, etc.) have a certain risk of drug resistance; hormone drugs are prone to cause irreversible damage such as telangiectasia; chemical peeling (fruit acid / salicylic acid) may cause skin stinging, redness and swelling, and is not suitable for sensitive skin.

[0004] In comparison, plant-derived acne-removing products are safe, have fewer side effects, and are suitable for long-term use and sensitive skin. However, single plant extracts also have market pain points such as instability of active ingredients such as polyphenols and flavonoids, easy oxidation and inactivation, affecting product shelf life; most plant active ingredients have large molecular weight and high lipophilicity, which limits their application in products; high content of unstable and difficult to partition from the lipid phase to the deeper hydrophilic dermis layer, penetrate into the deep layer of hair follicles, and have slow acne-removing effect.

[0005] In view of the above, it is necessary to develop a plant acne-removing composition that can synergistically act on multiple targets such as antibacterial, anti-inflammatory, oil control and repair, solve the stability defects of plant ingredients, have good immediate acne-removing effect, and be suitable for long-term safe use, to provide a safer and more effective solution for acne patients. SUMMARY

[0006] The present application aims to overcome the deficiencies of the prior art and provide a plant acne-removing composition, a preparation method and application thereof.

[0007] To achieve the above object, the technical scheme adopted by the present application comprises: In the first aspect, the present application provides a plant acne-removing composition, which comprises plant extracts and hydroxypropyl cyclodextrin; the plant extracts comprise the following components in parts by weight: Magnolia officinalis bark extract 0.1-4 parts, Glycyrrhiza extract 0.1-2 parts, Paeonia suffruticosa root bark extract 0.1-2 parts, and Stephania tetrandra extract 0.01-0.1 parts.

[0008] The main active components of Magnolia officinalis extract are Magnolol and Honokiol, which have the effects of antibiosis, anti-inflammation, and anti-oxidation. The main active components of Glycyrrhiza extract are triterpene saponins (4%-24%, mainly glycyrrhizic acid), total flavonoids (1%-5%), alkaloids, polysaccharides, and amino acids (1%-2%), which have the effects of anti-oxidation, anti-inflammation, anti-allergy, whitening, and sun protection. The main active components of Paeonia suffruticosa root bark extract are paeonol and paeoniflorin, which have the effects of anti-oxidation, anti-aging, whitening, and antibiosis. The main active component of Stephania tetrandra extract is tetrandrine, which has the effects of anti-allergy, anti-inflammation, analgesia, swelling reduction, redness and itching elimination, and skin immune system repair.

[0009] It is found through experiments that the complex plant extract described in the present application can inhibit the growth of acne-causing bacteria such as Propionibacterium acnes by the mutual cooperation of the four active components in the optimal amount ratio range, reduce the pathogenic bacteria that cause acne from the source, relieve the inflammatory reactions such as skin redness and pain caused by acne, quickly reduce the local discomfort, regulate the secretion of skin oil, reduce the problem of pore blockage caused by excessive secretion of sebum, and reduce the risk of acne recurrence, so as to achieve the effect of instant acne removal through the synergistic effect of multi-target points such as bacteriostasis, anti-inflammation, oil control, and repair.

[0010] In addition, the hydroxypropyl cyclodextrin in the composition can wrap the four plant extract components through its cyclic molecular structure, avoid the oxidation and degradation of the active components such as phenols in the extract due to factors such as light, high temperature, and oxygen, effectively improve the chemical stability of the composition, and the four plant extracts are mostly fat-soluble, direct mixing can easily cause stratification and precipitation, the hydrophilic outer layer of hydroxypropyl cyclodextrin can effectively improve the solubility of the four plant extracts in water-based formulations, and ensure the uniformity of the texture of the composition. In addition, the cyclic structure of hydroxypropyl cyclodextrin can assist the active components to penetrate the stratum corneum, improve the concentration of the active components in the deep layer of the skin, and further improve the acne-removing and oil-controlling effects of the composition.

[0011] Therefore, the plant acne-removing composition described in the present application can achieve instant and comprehensive acne-removing effect through multi-target synergistic effect, and the wrapping effect of hydroxypropyl cyclodextrin can significantly improve the stability of the composition, so as to ensure the continuous and effective product efficacy.

[0012] Preferably, the plant extract comprises the following components by weight: Magnolia officinalis bark extract 1-3.5 parts, Glycyrrhiza extract 0.5-1.5 parts, Paeonia suffruticosa root bark extract 0.5-1.5 parts, and Stephania tetrandra extract 0.02-0.06 parts.

[0013] Preferably, the plant extract comprises the following components by weight: Magnolia officinalis bark extract 2 parts, Glycyrrhiza extract 1 part, Paeonia suffruticosa root bark extract 1 part, and Stephania tetrandra extract 0.04 parts.

[0014] It has been found through experiments that when the four plant extracts are compounded in the optimal amount ratio defined above, the prepared composition has the best inhibitory effect on Propionibacterium acnes and the highest inhibitory rate on TNF-α, thereby achieving effective acne-removing effect through excellent antibacterial and anti-inflammatory effects.

[0015] Preferably, the mass ratio of the plant extract and hydroxypropyl cyclodextrin is (0.31-8.1):(3-7).

[0016] The amount ratio of the plant extract and hydroxypropyl cyclodextrin has an important influence on the solubility, stability, and acne-removing effect of the composition. If the amount of hydroxypropyl cyclodextrin is insufficient, the active components cannot be completely wrapped, causing oxidative degradation, and thus the acne-removing effect of the composition is reduced. If the amount of hydroxypropyl cyclodextrin is excessive, a large amount of empty hydroxypropyl cyclodextrin cannot participate in inclusion, causing waste of raw materials, and the excessive water-soluble hydroxypropyl cyclodextrin can destroy the system balance, causing the viscosity of the formula to increase and affecting the skin feel. It has been found through experiments that when the plant extract and hydroxypropyl cyclodextrin are compounded in the above-mentioned preferred amount ratio range, a water-soluble plant acne-removing composition with good stability can be obtained, which further enhances the acne-removing effect and is not limited by the product dosage form in subsequent application.

[0017] Preferably, the mass ratio of the plant extract and hydroxypropyl cyclodextrin is (2.02-6.56):(3-7).

[0018] More preferably, the mass ratio of the plant extract and hydroxypropyl cyclodextrin is 4.04:5.

[0019] In a second aspect, the present application provides a preparation method of the plant acne-removing composition, comprising the following steps: The Magnolia officinalis bark extract, Glycyrrhiza extract, Paeonia suffruticosa root bark extract, Stephania tetrandra extract, and hydroxypropyl cyclodextrin are uniformly mixed.

[0020] Preferably, the Magnoliae officinalis cortex extract, the Glycyrrhizae radix extract, the Paeoniae radix extract and the Stephaniae tetrandrae radix extract are obtained by conventional ethanol reflux extraction at a temperature of 80-90℃ for 1-3h, and then dried by concentration.

[0021] When the medicinal material is Magnoliae officinalis cortex, the solid-liquid ratio is 1:(7-9) and the ethanol concentration is 60-90vol.%; when the medicinal material is Glycyrrhizae radix, the solid-liquid ratio is 1:(9-11) and the ethanol concentration is 50-70vol.%; when the medicinal material is Paeoniae radix, the solid-liquid ratio is 1:(7-9) and the ethanol concentration is 40-60vol.%; and when the medicinal material is Stephaniae tetrandrae radix, the solid-liquid ratio is 1:(9-11) and the ethanol concentration is 60-80vol.%.

[0022] The skilled in the art can prepare the extracts according to the method of the present application or by choosing a common method by themselves, and theoretically, the extraction method has no significant effect on the efficacy of the extracts.

[0023] In a third aspect, the present application provides the use of the plant acne-removing composition in the preparation of an acne-removing product.

[0024] Preferably, the product comprises a cosmetic, and the cosmetic dosage form comprises at least one of cosmetic water, gel, essence, mask, emulsion and cream.

[0025] In a fourth aspect, the present application provides an acne-removing cosmetic comprising the plant acne-removing composition, and the mass percentage of the plant acne-removing composition in the acne-removing cosmetic is 0.5-4%.

[0026] Preferably, the acne-removing cosmetic further comprises an auxiliary material and water; the auxiliary material comprises at least one of an emulsifier, an emollient, a surfactant, a thickening agent, a humectant, an oil, a preservative, a chelating agent and a fragrance.

[0027] Preferably, the emulsifier comprises at least one of sorbitan isostearate, polysorbate-20, cetylstearyl alcohol, polysorbate-60, glyceryl stearate and PEG-100 stearate; and / or, the emollient comprises at least one of squalane, caprylic triglyceride, capric triglyceride, dimethicone and jojoba seed oil; and / or, the surfactant comprises at least one of sorbitan stearate, lecithin, stearic acid, sodium dodecylbenzenesulfonate, sodium dodecyl sulfate, polysorbate and glycerol fatty acid ester; and / or, the thickening agent comprises at least one of xanthan gum and carbomer; and / or, the humectant comprises at least one of glycerol, 1,3-propanediol, 1,2-hexanediol, butylene glycol, 1,2-pentanediol, allantoin and sodium hyaluronate.

[0028] Compared with the prior art, the present application has the following beneficial effects: (1) The thick bark extract, licorice extract, peony root bark extract and radix stephaniae tetrandrae extract are combined in a specific ratio in the present application, which has a significant multi-target synergistic effect on acne removal, can comprehensively intervene in the pathogenesis of acne from the aspects of bacteriostasis, anti-inflammation, regulation of sebum secretion, barrier repair and the like. (2) The composition solves the problems of poor permeability, poor absorption, difficulty in achieving instant acne removal and the like of traditional plant acne-removing products, significantly improves the solubility and bioavailability of fat-soluble active ingredients such as magnolol and paeonol in the plant extract by the hydrophobic cavity and hydrophilic outer wall of hydroxypropyl cyclodextrin, enhances the stability of the composition to make it suitable for various cosmetic systems, promotes transdermal absorption and reduces irritation; through synergistic matching of the components, the plant acne-removing composition with good instant acne-removing effect and suitable for long-term safe use is finally obtained. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 The instant acne-removing effect comparison chart before and after the subject uses the plant acne-removing gel for 24 hours; Figure 2 The long-acting acne-removing effect comparison chart before and after the subject uses the plant acne-removing gel for 14 days. DETAILED DESCRIPTION

[0030] In order to better illustrate the purposes, technical solutions and advantages of the present application, the present application will be further described below in combination with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present application and do not limit the present application.

[0031] The preparation methods of the extracts in the present application are as follows: (1) Preparation of the thick bark extract: the thick bark dry medicinal material is weighed, 75 vol.% ethanol aqueous solution is added according to a solid-liquid ratio of 1:8, and reflux extraction is carried out at 80 DEG C, 2 times, 1.5 h each time, to obtain an extract, which is dried to obtain the thick bark extract.

[0032] (2) Preparation of the licorice extract: the licorice dry medicinal material is weighed, 60 vol.% ethanol aqueous solution is added according to a solid-liquid ratio of 1:10, and reflux extraction is carried out at 85 DEG C, 2 times, 1 h each time, to obtain an extract, which is dried to obtain the licorice extract.

[0033] (3) Preparation of the peony root bark extract: the peony root bark dry medicinal material is weighed, 50 vol.% ethanol aqueous solution is added according to a solid-liquid ratio of 1:8, and reflux extraction is carried out at 90 DEG C, 2 times, 2 h each time, to obtain an extract, which is dried to obtain the peony root bark extract.

[0034] (4) Preparation of the extract of Stephania tetrandra S. Moore: The dried medicinal material of Stephania tetrandra S. Moore was weighed, 70 vol.% ethanol aqueous solution was added according to a material-to-liquid ratio of 1:10, and reflux extraction was performed at 90°C for 2 times, 1.5 h each time, to obtain an extract, which was dried to obtain the extract.

[0035] Examples 1-5 Examples 1-5 provide a plant acne-removing composition, the formulation of which is shown in Table 1. The preparation method of the composition comprises the following steps: The extract of Magnolia officinalis, the extract of Glycyrrhiza uralensis, the extract of Paeonia suffruticosa, the extract of Stephania tetrandra S. Moore, and the hydroxypropyl cyclodextrin were uniformly mixed.

[0036] Table 1 Formulation table of the composition of Examples 1-5 (parts by weight) Example 6 This example provides a plant acne-removing gel, which is prepared by using the plant acne-removing composition prepared in Example 1 as the active ingredient and collocating excipients. The formulation of the acne-removing gel is shown in Table 2.

[0037] Table 2 Formulation table of the acne-removing gel (mass percentage) The preparation process of the acne-removing gel is as follows: (1) The A phase was accurately weighed, heated in a water bath at 80°C, and uniformly homogenized for 5 min to obtain a uniform mixture, which was prepared for use; (2) The B phase was accurately weighed, uniformly mixed, and added to the A phase, which was uniformly stirred, cooled by stirring, and then the dissolved C1 phase was added, cooled to room temperature, and then the C2 phase was added for neutralization, which was uniformly stirred and discharged.

[0038] Comparative Examples 1-8 Comparative Examples 1-8 provide a plant acne-removing composition, the formulation of which is shown in Table 3.

[0039] Table 3 Formulation table of the composition of Comparative Examples 1-8 (parts by weight) Comparative Example 9 This comparative example provides a plant acne-removing composition, which is different from Example 1 only in that an equal amount of the extract of Scutellaria baicalensis Georgi is used instead of the extract of Paeonia suffruticosa.

[0040] Comparative Example 10 This comparative example provides a plant acne-removing composition, which is different from Example 1 only in that an equal amount of propylene glycol is used instead of hydroxypropyl cyclodextrin.

[0041] Effect Example 1 The plant acne-removing composition prepared in Examples 1-5 and Comparative Examples 1-2 and 10 is taken as a test sample, and the solubility and stability of the test sample are tested.

[0042] (1) Solubility test: The samples prepared in Examples 1-5 and Comparative Examples 1-2 and 10 are dissolved in water at a concentration of 1%, and the degree of dissolution is observed after magnetic stirring (500 rpm, 25°C) for 30 minutes. The specific results are shown in Table 4.

[0043] Precipitation rate = (precipitated mass / total mass of sample) x 100% Table 4 Sample Precipitation rate Water solubility determination Example 1 <1％ Complete dissolution Example 2 <1％ Complete dissolution Example 3 <1％ Complete dissolution Example 4 <1％ Complete dissolution Example 5 <1％ Complete dissolution Comparative Example 1 38.7％ Partial dissolution Comparative Example 2 89.6％ Trace dissolution Comparative Example 10 52.4％ Partial dissolution (2) Stability test: The samples prepared in Examples 1-5 and Comparative Examples 1-2 and 10 are dissolved in water at a concentration of 1%, and the state of the samples is observed after being placed in different test environments (room temperature (not protected from light), low temperature (-15±1°C), continuous light, and high temperature (45±1°C)) for 90 days and then being placed at room temperature to test the stability of the samples. The specific results are shown in Table 5.

[0044] Table 5 According to the results of the comparison between Examples 1 and Comparative Examples 1-2 and 10, hydroxypropyl cyclodextrin can encapsulate fat-soluble molecules in a hydrophobic cavity, block the destruction of active ingredients by external factors such as light, oxygen, and heat, effectively prevent the oxidative degradation of active molecules, and improve the solubility and stability of the composition. In Comparative Example 10, propylene glycol only solubilizes through hydrogen bonds and cannot prevent the agglomeration of fat-soluble components, i.e., the synergistic effect between the specific components in the present application cannot be achieved by conventional polyol solvents. When the amount ratio of the plant extract to hydroxypropyl cyclodextrin in Comparative Example 1 exceeds the limited range of the present application, the cyclodextrin encapsulation capacity is exceeded, resulting in an increase in free active components, which in turn affects the solubility and stability. Within the limited range of the present application, a water-soluble plant acne-removing composition with good stability can be obtained, thereby not being limited by the product dosage form in subsequent applications.

[0045] Effect Example 2 The plant acne-removing composition prepared in Examples 1-5 and Comparative Examples 3-9 is taken as a test sample, and the bacteriostatic and anti-inflammatory effects of the test sample are tested.

[0046] (1) Bacteriostatic effect test: According to the suspension quantitative bacteriostatic test in WS / T 650-2019 Antibacterial and Bacteriostatic Effect Evaluation Method 5.1.1, the inhibition rate of the samples in Examples 1-5 and Comparative Examples 3-9 on Propionibacterium acnes ATCC11827 is detected at a concentration of 0.5% for 1 h. The specific detection results are shown in Table 6.

[0047] Table 6 Sample Bacteriostatic rate Example 1 99.90％ Example 2 99.59％ Example 3 99.90％ Example 4 98.94％ Example 5 99.21％ Comparative Example 3 49.25％ Comparative Example 4 56.53％ Comparative Example 5 63.98％ Comparative Example 6 1.21％ Comparative Example 7 75.55％ Comparative Example 8 73.20％ Comparative Example 9 68.46％ The results of Table 6 show that the bacteriostatic rates of the compositions in the examples all reach more than 98%, indicating that the plant anti-acne compositions described in the present application have a strong inhibitory effect on P. acnes in a short time.

[0048] According to the results of Example 1 and Comparative Examples 3-9, the Magnolia officinalis bark extract, Glycyrrhiza extract, Paeonia extract and Stephania tetrandra extract have a significant synergistic effect on the inhibition of P. acnes. Among them, the Magnolia officinalis extract destroys the bacterial cell membrane and inhibits the formation of biofilm; the Glycyrrhiza extract interferes with the synthesis of bacterial lipids by inhibiting bacterial fatty acid metabolism enzymes, thereby enhancing the bacteriostatic effect; the Paeonia extract inhibits bacterial quorum sensing and reduces the secretion of virulence factors to prevent acne from worsening; the Stephania tetrandra extract inhibits bacterial proliferation through calcium antagonism, which can further reduce the risk of drug resistance. The effect of the composition described in the present application on inhibiting P. acnes is significantly better than that of single component, double component and three components, and when the amount ratio of Magnolia officinalis bark extract, Glycyrrhiza extract, Paeonia extract and Stephania tetrandra extract deviates slightly from the range defined in the present application, the bacteriostatic effect of the prepared composition is also significantly lower than that of the examples, indicating that the four active components described in the present application can better play a synergistic effect within a specific ratio range, and once the ratio exceeds the range, the synergistic effect between the components will be reduced or even lost, which will significantly weaken the instant acne-removing effect of the composition. In addition, the inhibition rate of the composition is also significantly reduced when the commonly used Huangqin extract with bacteriostatic effect is used instead of Paeonia extract, indicating that the specific plant extracts selected in the present application cannot be replaced conventionally, otherwise the synergism between the components will be affected, resulting in poor overall bacteriostatic effect. At the same time, although the amount of Stephania tetrandra extract added is small, it greatly improves the bacteriostatic performance of the entire composition.

[0049] (2) Anti-inflammatory effect test: The inhibition rate of TNF-α of the samples of Examples 1-5 and Comparative Examples 3-9 was detected according to T / SHRH 034-2020 “Cosmetic soothing effect test - in vitro TNF-α inflammatory factor content determination lipopolysaccharide induced macrophage cell line inflammatory cell model test method”.

[0050] The samples of Examples 1-5 and Comparative Examples 3-9 all do not affect the growth of cells at a concentration of 0.1 wt.%, so 0.1 wt.% concentration was selected for the experiment. The specific test results are shown in Table 7.

[0051] Table 7 Sample Inhibition rate Example 1 32.55％ Example 2 25.02％ Example 3 29.16％ Example 4 13.38％ Example 5 17.90％ Comparative Example 3 4.34％ Comparative Example 4 7.09％ Comparative Example 5 8.73％ Comparative Example 6 2.31％ Comparative Example 7 9.26％ Comparative Example 8 9.90％ Comparative Example 9 9.87％ The results in Table 7 show that the composition in the examples has a significant inhibitory effect on the inflammatory factor TNF-a, and the inhibitory effect of the composition on TNF-a is significantly better than that of single components, two components and three components, proving that the composition described in the application has a significant synergistic effect in anti-inflammatory. And after replacing the peony root bark extract with a plant extract also having anti-inflammatory efficacy, the inhibition rate decreased significantly, which further confirms that the four extracts are indispensable.

[0052] Effect Example 3 This effect example takes the anti-acne gel prepared in Example 6 as the test sample, and tests its anti-acne effect through human body trial.

[0053] (1) Safety test: Before the test, human skin patch test was carried out according to the human skin patch test method stipulated in the "Cosmetic Safety Technology Specification" (2015 edition), and the test results are shown in the observation of the skin condition of the sample group and the control group 1h, 24h and 48h after removing the test sample patch tester. The results show that no skin adverse reactions were observed in the sample group 1h, 24h and 48h, indicating that the anti-acne product prepared from the anti-acne composition of the application has low skin irritation, low sensitization and high safety.

[0054] (2) Instant anti-acne effect test: Select 10 people aged 18-35 with new inflammatory acne (diameter 2-5mm, redness score≥2 points) on the face without using antibiotics / Vitamin A drugs. Use the above 2% plant anti-acne gel for 8h and 24h, and test the same test site before and after use. The data results and evaluation results before and after use are compared by statistical test method to determine whether there is a statistical difference. The specific test method is as follows: 1. Pretreatment: stand for 30 minutes after cleansing (constant temperature and humidity environment: 22℃±1, RH 50%±5) 2. Product application: accurately apply 10mg of product (microsyringe to control the amount) to the acne area 3. Image acquisition: Equipment: VISIA-CR, skin surface oil tester Time points: 0h (baseline), 8h, 24h 4. Test index: red area, acne volume, skin oil content 5. Data statistics: descriptive statistics was performed on the measurement values, including quantity, mean, standard deviation, minimum value, median value, and maximum value, etc. The difference between the initial value of each test area and the measurement value at other time points, the change rate, and the measurement value at each time point were used to statistically analyze the difference between different time points. Self- before and after comparison was used, and if the data was normally distributed, paired T test was used to analyze the difference between the two groups of data. If the data was not normally distributed, rank sum test was used to analyze the difference between the two groups of data. Double-tailed test was used in the statistical method, and the test level was a = 0.05. The specific test results are shown in Table 8.

[0055] Table 8 The results of Table 8 show that after using the 2% plant acne-removing gel according to the present application for 8h, the skin redness is significantly reduced by 16.20% (P<0.001), the acne volume is significantly reduced by 29.25% (P<0.05), and the oil secretion is also significantly reduced by 15.74% (P<0.05); after 24h, the skin redness is significantly reduced by 21.84% (P<0.01), the acne volume is significantly reduced by 52.31% (P<0.05), and the oil secretion is also significantly reduced by 19.57% (P<0.05). The test results show that the acne-removing gel prepared from the acne-removing composition according to the present application can quickly reduce swelling and redness, inhibit oil secretion, and achieve the effect of instant acne removal in a short time. Figure 1 A comparison chart of instant acne-removing effect before and after using the plant acne-removing gel for 24h for a subject.

[0056] (2) Long-acting acne-removing effect test: According to the “Q / CX 001-2022 Test Method for Acne-removing Effect of Cosmetics”, 30 subjects aged 18-60 years with facial skin of oily or mixed oily type (10 males and 20 females) were selected. The above-mentioned 2% plant acne-removing gel was used for 14 days, and the same test site before and after use was tested. The data results and evaluation results before and after use were compared by statistical test method, so as to determine whether there was a statistical difference.

[0057] 1. Test indexes are as follows: (1) Facial lesion count The researcher counted the lesions (total of acne, papules, pustules, nodules, and cysts) according to the lesion photos of the subjects.

[0058] (2) ISGA score The researcher scored according to the ISGA scale according to the lesion photos of the subjects.

[0059] Detection process: ① Cleanse; ② Wait for 30 minutes; ③ Take a photo of the acne side of the face with a mobile phone; ④ Repeat ①-③ at any time point 7-14 days after use; ⑤ The researcher quantitatively evaluates according to the photo.

[0060] (3) Sebum At each visit, the test site is tested with a skin surface oil tester, 1 time for each test site, and recorded. If the value measured by the oil absorption method of the extinction band is ≥200 μg / cm 2 The second test is performed on the same test site, and the oil content is the sum of the values of the two times.

[0061] 2. The statistical method is as follows: Using SPSS analysis software, the data after use is compared with the base value, the significance test of normal distribution of the data improvement value is performed by Shapiro-Wilk Test, P>0.05, then normal distribution is performed, paired t test is performed, and the significant difference level α is taken as 0.05. If P<0.05, then non-normal distribution is performed, rank sum test is performed, and the significant difference level α is taken as 0.05.

[0062] The percentage of lesion reduction (%) = (ISGA score before use- ISGA score after use) / ISGA score before use x 100%; The lesion regression rate (%) = (the number of lesions before use-the number of lesions after use) / the number of lesions before use x 100%.

[0063] Evaluation criteria: recovery: lesion regression rate ≥90%; significant effect: lesion regression rate 60%-89%; improvement: lesion regression rate 20%-59%; ineffective: lesion regression rate ≤20%.

[0064] Table 9 is the ISGA scale, and Table 10 is the specific test results.

[0065] Table 9

[0066] Table 10 The results of Table 10 show that the lesion count and ISGA score before and after use are statistically improved. The percentage of lesion reduction is 72.06%, the lesion regression rate is 76.59% (P<0.001), and the amount of sebum secretion is also significantly reduced by 25.42% (P<0.05). The test results show that the acne-removing gel prepared from the acne-removing composition has significant acne-removing effect on a large area of acne while being safe and mild in long-term use. Figure 2 The long-acting acne-removing effect comparison chart of a subject before and after using the plant acne-removing gel for 14 days. The long-acting acne-removing effect comparison chart of a subject before and after using the plant acne-removing gel for 14 days.

[0067] In summary, the plant acne-removing composition of the present application mixes Magnolia officinalis bark extract, Glycyrrhiza extract, Paeonia suffruticosa root bark extract, Stephania tetrandra extract and hydroxypropyl cyclodextrin in a specific range, improves solubility and stability, and makes it suitable for various cosmetic systems. The components can play a synergistic effect, inhibit bacteria, anti-inflammatory, regulate sebum secretion, repair the barrier, and comprehensively intervene in the pathogenesis of acne from multiple aspects, and effectively improve the defects of many plant active ingredients (such as polysaccharides, flavonoids) that are difficult to penetrate the stratum corneum, resulting in limited local effect. Promote transdermal absorption, reduce irritation, optimize the bioavailability of each component, and ultimately obtain a plant acne-removing composition with good immediate acne-removing effect and suitable for long-term safe use.

[0068] Finally, it should be noted that the above examples are intended to illustrate the technical solutions of the present application rather than limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A plant-based acne-removing composition, characterized in that, The acne-removing composition comprises plant extracts and hydroxypropyl cyclodextrin; the plant extracts comprise the following components in parts by weight: 0.1-4 parts Magnolia officinalis bark extract, 0.1-2 parts Glycyrrhiza uralensis extract, 0.1-2 parts Paeonia suffruticosa root bark extract, and 0.01-0.1 parts Stephania tetrandra extract.

2. The plant-based acne-removing composition as described in claim 1, characterized in that, The plant extract comprises the following components in parts by weight: 1-3.5 parts Magnolia officinalis bark extract, 0.5-1.5 parts Glycyrrhiza uralensis extract, 0.5-1.5 parts Paeonia suffruticosa root bark extract, and 0.02-0.06 parts Stephania tetrandra extract.

3. The plant-based acne-removing composition as described in claim 2, characterized in that, The plant extract comprises the following components in parts by weight: 2 parts Magnolia officinalis bark extract, 1 part Glycyrrhiza uralensis extract, 1 part Paeonia suffruticosa root bark extract, and 0.04 parts Stephania tetrandra extract.

4. The plant-based acne-removing composition as described in claim 1, characterized in that, The mass ratio of the plant extract to hydroxypropyl cyclodextrin is (0.31-8.1):(3-7).

5. The method for preparing the plant-based acne-removing composition according to any one of claims 1-4, characterized in that, Includes the following steps: Simply mix the Magnolia officinalis bark extract, licorice extract, Paeonia suffruticosa root bark extract, Stephania tetrandra extract, and hydroxypropyl cyclodextrin evenly.

6. The use of the plant-based acne-removing composition according to any one of claims 1-4 in the preparation of acne-removing products.

7. The application as described in claim 6, characterized in that, The product includes cosmetics, and the cosmetic dosage form includes at least one of toner, gel, serum, mask, lotion, and cream.

8. An acne-removing cosmetic product, characterized in that, The acne-removing cosmetic product includes the plant-based acne-removing composition according to any one of claims 1-4, wherein the plant-based acne-removing composition has a mass percentage content of 0.5-4% in the acne-removing cosmetic product.

9. The acne-removing cosmetic product as described in claim 8, characterized in that, The acne-removing cosmetic also includes excipients and water; the excipients include at least one of emulsifiers, emollients, surfactants, thickeners, moisturizers, oils, preservatives, chelating agents, and fragrances.

10. The acne-removing cosmetic product as described in claim 9, characterized in that, The emulsifier comprises at least one of sorbitan isostearate, polysorbate-20, cetearyl alcohol, polysorbate-60, glyceryl stearate, and PEG-100 stearate; and / or, the emollient comprises at least one of squalane, caprylic triglyceride, caprylic triglyceride, polydimethylsiloxane, and jojoba seed oil; and / or, the surfactant comprises at least one of sorbitan stearate, lecithin, stearic acid, sodium laurylbenzene sulfonate, sodium lauryl sulfate, polysorbate, and fatty acid glycerides; and / or, the thickener comprises at least one of xanthan gum and carbomer; and / or, the humectant comprises at least one of glycerin, 1,3-propanediol, 1,2-hexanediol, butylene glycol, 1,2-pentanediol, allantoin, and sodium hyaluronate.

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