Whitening composition containing jasmine extract as well as preparation method and application of whitening composition

Abstract

The invention provides a whitening composition containing a jasmine extract as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The whitening composition is prepared from the following components in parts by weight: 1 to 10 parts of nicotinamide; 0.1 to 5 parts of taurine; 0.1 to 3 parts of lecithin; 0.01 to 2 parts of inositol; 0.01 to 1 part of ceramide NP; 0.1 to 3 parts of tranexamic acid; 0.01-2 parts of glutathione, 0.1-5 parts of a jasmine flower lactobacillus fermentation extract and 20-60 parts of water. By controlling the types and mass ratio of the specific components, especially the mass ratio of lecithin to tranexamic acid, inositol to glutathione, and nicotinamide to taurine to jasmine lactic acid bacteria fermentation extract, the obtained composition not only can inhibit melanin generation and transport, but also can promote permeation of effective components, so that the anti-aging effect of the composition is improved, and the anti-aging effect of the composition is improved. The content of effective components in the skin is increased, so that the absorption effect and the bioavailability of the product are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a whitening composition containing jasmine extract, a preparation method and application. BACKGROUND

[0002] Melanin is mainly produced by melanocytes in the basal layer of the epidermis of human skin, which can reduce the damage of ultraviolet light to the skin. The content and distribution of melanin determine the color of the skin. However, abnormal accumulation of melanin in the basal layer can cause hyperpigmentation, leading to melasma, freckles, age spots and other conditions, affecting people's quality of life. With the continuous deepening of the study of melanin biosynthesis, researchers have found that tyrosinase plays an important role in melanin biosynthesis. It is the main rate-limiting enzyme in the process of converting tyrosine and dopa to melanin. Its overexpression is the main cause of hyperpigmentation. Therefore, inhibiting the activity of tyrosinase can block the reaction chain of melanin biosynthesis and reduce the generation of melanin, achieving the effect of whitening.

[0003] Based on the pathway and influencing factors of melanin production, the main approaches to skin whitening and skin care include the following aspects: (1) reducing the number of melanocytes by inhibiting the proliferation of skin melanocytes; (2) reducing melanin biosynthesis by inhibiting the catalytic activity of tyrosinase, the key enzyme for skin melanin production, to achieve the purpose of whitening; (3) preventing the damage of ultraviolet light in sunlight to the skin (tanning and sunburn) to slow down skin aging; (4) removing oxygen free radicals in the skin by using antioxidants to slow down melanin production and slow down skin aging.

[0004] Based on the above influencing factors, Chinese patent CN108498413A discloses a cosmetic composition containing a whitening compound, which comprises 1-3% of nicotinamide, 1-4% of a whitening compound, 0.5-1.5% of citrus peel extract, 0.1-0.5% of arbutin and 0.1-5% of umbellate artemisia extract, etc. The components of the whitening compound are water, butylene glycol, arbutin, citric acid, sodium sulfite, acetyl tyrosine, strawberry saxifrage extract, peony root extract, ascorbyl glucoside, radix scutellariae extract, glutathione and hydrolyzed cherry plum. The characteristics of this invention are that the raw materials selected at each whitening node have synergistic effect, which has synergistic whitening effect; it can not only inhibit the activity of tyrosinase and inhibit the transfer of melanin, but also brighten the skin color, and also has a synergistic effect at each node in the whitening path, finally achieving a safe, effective and stable whitening effect.

[0005] For example, Chinese patent CN104027293A discloses a whitening cosmetic and a preparation method thereof. The invention is mainly composed of a variety of plant compositions as main raw materials, mainly including rice bran extract, rhodiola extract, ginkgo leaf extract, tea polyphenol, lemon juice concentrate, chitosan, pueraria extract, konjac flour, sunflower disc extract, sunflower seed extract, soap nut, hemicellulose, honey; in addition, it also contains a small amount of glycerol, 1,3 propylene glycol, squalane, sodium hyaluronate, carbomer, triethanolamine, allantoin, nano titanium dioxide, collagen powder, antibacterial agent, isooctyl ferulate and glutathione. The composition can be used in whitening formulations, and has the effects of whitening skin, moisturizing, antibacterial and anti-inflammatory, removing free radicals, inhibiting melanin formation, inhibiting dopachrome tautomerase and tyrosinase activity.

[0006] However, the existing composition contains a variety of components, and the large amount of mixed functional components sometimes causes problems in use and safety. Therefore, it is necessary to develop a composition with simple components and better whitening effect, which can inhibit tyrosinase activity and brighten skin color, and a preparation method thereof. SUMMARY

[0007] Based on the problems in the prior art, the present invention aims to provide a whitening composition, a preparation method and an application with jasmine extract as the functional component.

[0008] In order to achieve the above-mentioned purpose, the technical scheme adopted by the present invention is as follows: A whitening composition containing jasmine extract, which comprises the following components by weight fraction: nicotinamide 1-10 parts; taurine 0.1-5 parts; lecithin 0.1-3 parts; inositol 0.01-2 parts; ceramide NP 0.01-1 part; tranexamic acid 0.1-3 parts; glutathione 0.01-2 parts, jasmine flower lactic acid bacteria fermented extract 0.1-5 parts and water 20-60 parts.

[0009] Preferably, the whitening composition comprises the following components by weight fraction: nicotinamide 2-9 parts; taurine 0.5-4.5 parts; lecithin 0.5-2.8 parts; inositol 0.1-1.8 parts; ceramide NP 0.02-0.08 parts; tranexamic acid 0.5-2.8 parts; glutathione 0.05-1.8 parts, jasmine flower lactic acid bacteria fermented extract 0.5-4.5 parts and water 40-55 parts.

[0010] Preferably, the whitening composition comprises, by weight parts: niacinamide 3-8 parts; taurine 1-4.5 parts; lecithin 1-2.5 parts; inositol 0.5-1.5 parts; ceramide NP 0.03-0.07 parts; tranexamic acid 0.8-2.2 parts; glutathione 0.1-1.5 parts, jasmine lactic acid bacteria fermented extract 1-4 parts and water 40-50 parts.

[0011] Further preferably, the whitening composition comprises, by weight parts: niacinamide 4-8 parts; taurine 1.5-3.5 parts; lecithin 1.5-2.2 parts; inositol 0.8-1.2 parts; ceramide NP 0.04-0.06 parts; tranexamic acid 1-2 parts; glutathione 0.5-1 parts, jasmine lactic acid bacteria fermented extract 2-3 parts and water 40-45 parts.

[0012] Still further preferably, the whitening composition comprises, by weight parts: niacinamide 5-8 parts; taurine 2-3 parts; lecithin 1.8-2 parts; inositol 0.8-1.0 parts; ceramide NP 0.05-0.06 parts; tranexamic acid 1.5-1.8 parts; glutathione 0.6-0.8 parts, jasmine lactic acid bacteria fermented extract 2-2.5 parts and water 40-45 parts.

[0013] Further preferably, the niacinamide is selected from any value or range between 1-10 parts by weight, in particular from: 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts or any range between any two of them.

[0014] The taurine is selected from any value or range between 0.1-5 parts by weight, in particular from: 0.1 part, 0.2 part, 0.3 part, 0.5 part, 1 part, 1.5 part, 2 part, 2.5 part, 3 part, 3.5 part, 4 part, 4.5 part, 5 part or any range between any two of them.

[0015] The lecithin is selected from any value or range between 0.1-3 parts by weight, in particular from: 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.8 part, 1 part, 1.2 part, 1.5 part, 1.8 part, 2.0 part, 2.2 part, 2.5 part, 2.8 part, 3.0 part or any range between any two of them.

[0016] The myo-inositol is selected from any value or range between 0.01-2 parts by weight, and specifically can be selected from: 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, or a range between any two of them.

[0017] The ceramide NP is selected from any value or range between 0.01-1 parts by weight, and specifically can be selected from: 0.01 parts, 0.03 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.3 parts, 0.5 parts, 0.8 parts, 1 parts, or a range between any two of them.

[0018] The tranexamic acid is selected from any value or range between 0.1-3 parts by weight, and specifically can be selected from: 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2.0 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3.0 parts, or a range between any two of them.

[0019] The glutathione is selected from any value or range between 0.01-2 parts by weight, and specifically can be selected from: 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, or a range between any two of them.

[0020] The jasmine lactic acid bacteria fermented extract is selected from any value or range between 0.1-5 parts by weight, and specifically can be selected from: 0.1 parts, 0.2 parts, 0.3 parts, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, or a range between any two of them.

[0021] As some preferred embodiments, the mass ratio of the lecithin and the tranexamic acid is 1:1; the mass ratio of the myo-inositol and the glutathione is 1:1.

[0022] It is unexpectedly found in the implementation of the present application that the addition of lecithin, tranexamic acid, myo-inositol and glutathione in the components and the control of the mass ratio between the components not only make the composition have better whitening effect, but also promote the penetration of the effective components in the product, increase the content of the effective components in the skin, thereby improving the absorption effect and bioavailability of the product.

[0023] As some preferred embodiments, the mass ratio of the nicotinamide, taurine and the jasmine lactic acid bacteria fermented extract is 2-5:1.5:1. Preferably, the mass ratio of the nicotinamide, taurine and the jasmine lactic acid bacteria fermented extract is 2-4:1.5:1. Further preferably, the mass ratio of the nicotinamide, taurine and jasmine lactic acid bacteria fermented extract is 4:1.5:1.

[0024] As a preferred embodiment, the whitening composition comprises the following components by weight fraction: nicotinamide 8 parts; taurine 3 parts; lecithin 1.8 parts; inositol 0.8 parts; ceramide NP 0.05 parts; tranexamic acid 1.8 parts; glutathione 0.8 parts, jasmine lactic acid bacteria fermented extract 2 parts and water 40 parts.

[0025] In the implementation of the present application, lecithin, tranexamic acid, inositol and glutathione are used in combination, and by controlling the mass ratio between the components, not only can the synthesis pathway of melanin be inhibited and the whitening effect be enhanced, but also the stability of the composition can be significantly improved; nicotinamide, taurine and jasmine lactic acid bacteria fermented extract are mixed, and by controlling the mass ratio, not only can the generation and transport of melanin be inhibited while improving tolerance and repairing the skin barrier, but also the irritation of the product can be significantly reduced, so that the prepared composition not only has a better whitening effect, but also helps to repair the skin barrier, so that the skin has better stability.

[0026] The present application also provides a preparation method of the above-mentioned whitening composition, comprising the following steps: First, nicotinamide, taurine, lecithin, inositol, ceramide NP, tranexamic acid and glutathione are added to water, and after being mixed uniformly, jasmine lactic acid bacteria fermented extract is added and continues to be mixed until uniform, thereby obtaining the whitening composition.

[0027] The present application also provides the use of the above-mentioned whitening composition in the preparation of a cosmetic with whitening effect.

[0028] A cosmetic with whitening effect, which comprises the above-mentioned whitening composition and a cosmetically acceptable auxiliary material.

[0029] The auxiliary material is selected from one or more of a filler, an emulsifying agent, an emollient, a pH adjusting agent, a suspending agent, a stabilizer and a humectant.

[0030] As preferred, the cosmetic is a spray.

[0031] Compared with the prior art, the present application has the following beneficial effects: Existing whitening cosmetics mostly use an emulsion system as a carrier, and usually have the problems of large molecular weight of the base, low skin barrier permeability, poor compatibility of active substances with the carrier or unobvious whitening effect.

[0032] The application can not only inhibit the synthesis of melanin and enhance the whitening effect, but also significantly improve the stability of the composition by mixing lecithin, tranexamic acid, inositol and glutathione in a specific ratio during implementation; mixing nicotinamide, taurine and jasmine lactic acid bacteria fermented extract and controlling the mass ratio can not only inhibit the generation and transport of melanin, but also improve tolerance, repair the skin barrier, and significantly reduce the irritation of the product, so that the prepared composition not only has better whitening effect, but also helps to repair the skin barrier.

[0033] The composition prepared by the application is verified by Raman spectroscopy, and after continuous action for 6 hours, characteristic spectral peak signals can be detected in the dermis layer area (about 150-200 mu m deep), proving that the active components can effectively penetrate the stratum corneum and diffuse to the dermis; compared with traditional emulsion type whitening products, the composition provided by the application can realize active directional penetration without damaging the integrity of the skin barrier, ensuring that the ingredients continuously play the roles of antioxidant, anti-glycation and melanin inhibition in the deep layer of the skin, thereby bringing a faster and more lasting brightening improvement effect; that is, the application overcomes the problems of low penetration efficiency, shallow action layer and short efficacy maintenance time of existing whitening body milk, and realizes a breakthrough improvement from surface brightening to deep brightening. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 Raman spectrum of human in vivo skin before using the sample; Raman characteristic peaks are located at: 943 cm -1 , 1275 cm -1 , 1455 cm -1 , 1655 cm -1 , 2846 cm -1 , 2883 cm -1 , 2934 cm -1 and 3226 cm -1 ; Figure 2 Raman spectrum of nicotinamide; Raman characteristic peaks are located at: 624 cm -1 , 787 cm -1 , 836 cm -1 , 1040 cm -1 , 1122 cm -1 , 1160 cm -1 , 1209 cm -1 , 1389 cm -1 , 1579 cm -1 , 1593 cm -1 , 1675 cm -1 , 3066 cm-1 and 3099 cm -1 ; Figure 3 The content distribution image of nicotinamide at different depths of the skin at different times after using the sample of Example 3; Figure 4 The representative test images of the zebrafish embryos of each group; Note: The yellow selected line represents the gray scale collection area of melanin. DETAILED DESCRIPTION

[0035] In order to make the technical means, creative features, purposes and effects of the present application easy to understand, the following specific embodiments are further described. However, the following embodiments are only preferred embodiments of the present application, and are not all. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application. In the following embodiments, if not otherwise specified, the operation method used is a conventional operation method, the equipment used is a conventional equipment, and the equipment materials used in each embodiment are the same, and each component is a conventional component that can be obtained by commercial purchase.

[0036] The purchase of the plant extracts in the following embodiments is shown in Table 1 below: Table 1

[0037] Example 1: A whitening composition containing jasmine extract and a preparation method thereof The following components are included by weight fraction: nicotinamide 4 parts; taurine 1.5 parts; lecithin 1 part; inositol 0.1 part; ceramide NP 0.1 part; tranexamic acid 1 part; glutathione 0.1 part, jasmine lactic acid bacteria fermented extract 1 part, and water 20 parts.

[0038] Preparation method: First, nicotinamide, taurine, lecithin, inositol, ceramide NP, tranexamic acid and glutathione are added to water, mixed uniformly, and then the jasmine lactic acid bacteria fermented extract is added and mixed until uniform, to obtain the whitening composition.

[0039] Example 2: A whitening composition containing jasmine extract and a preparation method thereof The following components are included by weight fraction: nicotinamide 9 parts; taurine 4.5 parts; lecithin 2.8 parts; inositol 2 parts; ceramide NP 0.08 parts; tranexamic acid 2.8 parts; glutathione 2 parts, jasmine lactic acid bacteria fermented extract 3 parts, and water 60 parts.

[0040] Preparation method: The nicotinamide, taurine, lecithin, inositol, ceramide NP, tranexamic acid and glutathione are first added into water, mixed uniformly, then the jasmine flower lactic acid bacteria fermentation extract is added and mixed until uniform, to obtain the whitening composition.

[0041] Example 3: A whitening composition containing jasmine extract and a preparation method thereof The following components are included by weight fraction: 8 parts of nicotinamide; 3 parts of taurine; 1.8 parts of lecithin; 0.8 parts of inositol; 0.05 parts of ceramide NP; 1.8 parts of tranexamic acid; 0.8 parts of glutathione, 2 parts of jasmine flower lactic acid bacteria fermentation extract and 40 parts of water.

[0042] Preparation method: The nicotinamide, taurine, lecithin, inositol, ceramide NP, tranexamic acid and glutathione are first added into water, mixed uniformly, then the jasmine flower lactic acid bacteria fermentation extract is added and mixed until uniform, to obtain the whitening composition.

[0043] Comparative Example 1: The difference from Example 3 is that lecithin is not added, the content of tranexamic acid is increased to 3.6 parts, and the others are the same as Example 3.

[0044] Comparative Example 2: The difference from Example 3 is that glutathione is not added, the content of inositol is increased to 1.6 parts, and the others are the same as Example 3.

[0045] Comparative Example 3: The difference from Example 3 is that the mass ratio of nicotinamide, taurine and jasmine flower lactic acid bacteria fermentation extract is 3:1:8, i.e. 3 parts of nicotinamide; 1 part of taurine; 8 parts of jasmine flower lactic acid bacteria fermentation extract, and the others are the same as Example 3.

[0046] Effect experiment: 1. Irritation test - acute eye irritation test Test material: samples prepared in Examples 1-3 and Comparative Examples 1-3; Experimental animals: New Zealand rabbits (ordinary level, female, body weight 2.0-2.5 kg), 3 in each group, divided into 6 groups, animal qualification certificate number: NO.44411600016326.

[0047] Source of animals: purchased from Guangdong Medical Experimental Animal Center (Sanshui Base), experimental animal production license number: SCXK (Yue) 2023-0035.

[0048] Housing environment: ordinary environment, temperature 21.3-22.7℃, relative humidity 64.3-65.2 RH%, experimental animal environment use license number: SYXK (Yue) 2023-0216.

[0049] Feed: Provided by Linyi Shengquan Oil and Fat Chemical Co., Ltd. Jiangquan Feed Factory, License No.: Lu Shi Zhen (2024) 16062, Quality Certificate No.: NO. 20240923000284.

[0050] The above-mentioned animals were examined (including using sodium fluorescein) for 24 hours before the test, and no eye irritation symptoms were found.

[0051] Test method: Take the above-mentioned animals, gently pull the lower eyelid of the right eye of the rabbit, and drop 100 mg of the test substance into the conjunctival sac, so that the upper and lower eyelids are passively closed for 1 s. Do not flush the eyes within 24 hours after dropping the test substance, and do not treat the left eye as a self-control.

[0052] At 1 h, 24 h, 48 h, 72 h, and 4 d and 7 d after dropping the test substance, the eyes of the animals were examined using a magnifying glass and a pupil pen lamp, and at the same time, after observing and recording the results at 24 h, the eyes of all animals were examined using sodium fluorescein in each examination, and the eye irritation reaction was recorded and scored according to Table 1 of Chapter 6, Part 5 of the Cosmetic Safety Technical Specification (2015 Edition). If no irritation reaction is found at 72 h, the test can be terminated; if cumulative corneal or other eye irritation is found, which does not recover within 7 d, the observation time needs to be extended to 21 d, and an observation report needs to be provided.

[0053] After detection, the results of the rabbit acute eye irritation test of the test substance were as follows: the whitening composition prepared in Examples 1-3 was tested under non-flushing conditions, and the highest mean score of the test substance on the rabbit eye irritation was 0.00 for the cornea, 0.00 for the iris, and 0.00 for the conjunctiva, which was non-irritating; the whitening composition prepared in Comparative Examples 1-2 was tested under non-flushing conditions, and the highest mean score of the test substance on the rabbit eye irritation was 0.00 for the cornea, 0.00 for the iris, and 0.00 for the conjunctiva, which was non-irritating; the whitening composition prepared in Comparative Example 3 was tested under non-flushing conditions, and the highest mean score of the test substance on the rabbit eye irritation was 0.00 for the cornea, 0.00 for the iris, and 2.00 for the conjunctiva, which appeared to be slightly swollen, and was slightly irritating.

[0054] 2. Penetration rate detection Test purpose: The human (in vivo) Raman non-invasive optical test method was used to study the penetration effect of nicotinamide in the human skin in the sample at 0.5 h, 1 h, 2 h and 6 h after using the sample by different methods.

[0055] Test method: Select a 1cm x 1cm area at the front end of the human arm for testing: test samples are compositions prepared in Examples 1-3 and Comparative Examples 1-3, and test at 0.5h, 1h, 2h and 6h time points on the skin in the test area, and calculate the relative penetration rate of nicotinamide in the sample at each time point after using the sample.

[0056] Test instrument: LabRAM Odyssey high-speed high-resolution microconfocal Raman spectrometer (HORIBA).

[0057] Test environment: Environmental requirements: temperature: 22℃±2℃; humidity: 50%RH±10%RH.

[0058] Test process: The test subjects are explained, and after the subjects visit, the skin in the test area of the arm is cleaned with water, and after cleaning, the subjects enter the constant temperature and humidity room and sit for 30 min, and after 30 min, the subjects participating in the test are used according to the test requirements, and after 0.5h, 1h, 2h and 6h, the human in-vivo Raman test is carried out.

[0059] The penetration behavior of the product is confirmed by the characteristic Raman signal of the product that is different from the intrinsic signal of the skin, and the distribution in the different skin depth space is confirmed.

[0060] The data normal distribution test is carried out by Shapiro-Wilk Test using ORIGIN software, and the paired t test is used if it is normally distributed, otherwise the sum test of two related samples is used.

[0061] Relative penetration rate (%) = normalized (change of Raman characteristic peak caused after using the test product) x 100%.

[0062] The relative penetration rate test results are shown in Table 2 below.

[0063] Table 2

[0064] According to the test results in Table 2 above and Figure 3 The test results show that the whitening composition prepared in the application has better penetration, and the characteristic spectral peak signal can be detected in the dermis layer area after 6 hours of continuous action, that is, the active components of the whitening composition of the application can effectively penetrate the stratum corneum and diffuse to the dermis; and in Comparative Example 1 and Comparative Example 2, lecithin and glutathione are omitted, which will affect the penetration of the effective components in the product to a certain extent, thereby reducing the content of the effective components in the skin; in Comparative Example 3, the mass ratio between the components is changed, which also affects the penetration of the effective components, thereby reducing the content of the effective components penetrating into the skin.

[0065] 3. Whitening and freckle-removing efficacy detection - Zebrafish embryo melanin production inhibition rate test Test principle: Human skin melanocytes can produce tyrosinase, which can convert tyrosine into dopa pigment, which in turn forms melanin. Melanin is transmitted to the surrounding epidermal cells by melanocytes, causing the skin to darken. Zebrafish embryos start to form skin melanin at 24 hours of development, and the mechanism of skin melanin production is consistent with that of humans. By giving the test substance during the process of zebrafish embryo skin melanin formation, the inhibition of skin melanin is observed under a microscope to evaluate whether the test substance has whitening and freckle-removing efficacy.

[0066] Experimental organisms: Zebrafish embryos developed to 24 hours were selected as experimental organisms.

[0067] Reagent consumables: Main reagents: including N-phenylthiourea (positive drug PTU), methyl cellulose, MS-222, streptomyces protease, calcium chloride, magnesium sulfate, sodium bicarbonate, potassium chloride, etc. Main consumables: including 6 / 12 hole plates, 1.5mL / 10mL / 50mL centrifuge tubes, disposable Pasteur pipettes, disposable culture dishes.

[0068] Instrument equipment: Nikon upright microscope, analytical balance (one ten thousandth), pipette, water quality detection equipment (dissolved oxygen meter, salinity meter, hardness meter, pH meter), refrigerator, zebrafish breeding system, constant temperature incubator, etc.

[0069] Test procedure: Test sets blank control group, positive drug group (0.009 ‰) and sample group (5.0 g / L) respectively, each group has 3 biological repeats, each repeat sets 8 zebrafish embryos, and 5 mL of working solution corresponding to each concentration group is added to each hole; place each test group in a 28.5±0.5℃ constant temperature incubator for 24 hours; remove the membrane with 1mg / mL streptomyces protease solution; after fixing with 3% methyl cellulose, observe and take pictures under a stereomicroscope. When taking pictures, the zebrafish embryo should have its head to the left, abdomen to the bottom, and body kept horizontal. The picture results of all zebrafish embryos must be taken under the same instrument and environmental conditions, and the body position of zebrafish embryos must be kept consistent. Image J software is used to analyze the melanin gray value of each, and the difference significance test is performed on the data of each group.

[0070] Settlement: Calculate the whitening and freckle-removing effect of the test substance (expressed as melanin inhibition rate), the formula is as follows: Melanin production inhibition rate (%) = [(S1 S2) / S1] x 100% (1) S2 - average value of melanin gray value of zebrafish embryos in sample group; S1 - average value of melanin gray value of zebrafish embryo in blank control group; The specific detection results are shown in Table 3 below.

[0071] Table 3: Statistics of melanin gray value of zebrafish embryo in each group (n = 20)

[0072] According to the detection results in Table 3 above, compared with the blank control group, the melanin inhibition rate of the sample prepared in Example 1-3 at a concentration of 5.0 g / L on zebrafish embryo is more than 18%, especially the melanin inhibition rate of the sample prepared in Example 3 can reach 20.17%, which indicates that the whitening composition provided by the present application can significantly inhibit the melanin production of zebrafish embryo, and the test results show that the tested substance has the effect of whitening and removing spots. The whitening and spot-removing effect of the composition obtained by omitting lecithin or glutathione in Comparative Example 1-2 is obviously reduced, and the melanin inhibition rate is less than 15%, and changing the mass ratio of nicotinamide, taurine and lactic acid bacteria fermented extract of jasmine flowers in Comparative Example 3 also affects the whitening and spot-removing effect of the composition, that is, only the composition of the present application can achieve better whitening and spot-removing effect.

[0073] The above examples are only used to illustrate the technical solutions of the present application but not to limit it, and although the present application has been described in detail with reference to the above examples, the ordinary skilled in the art can still modify or equivalently replace the specific implementation solutions of the present application without departing from the spirit and scope of the present application, and any modification or equivalent replacement without departing from the spirit and scope of the present application is within the protection scope of the claims of the present application.

Claims

1. A whitening composition containing jasmine extract, characterized in that: The product comprises the following components by weight: 1-10 parts nicotinamide; 0.1-5 parts taurine; 0.1-3 parts lecithin; 0.01-2 parts inositol; 0.01-1 part ceramide NP; 0.1-3 parts tranexamic acid; 0.01-2 parts glutathione; 0.1-5 parts jasmine lactic acid bacteria fermentation extract; and 20-60 parts water.

2. The whitening composition according to claim 1, characterized in that: The product comprises the following components by weight: 3-8 parts nicotinamide; 1-4 parts taurine; 1-2.5 parts lecithin; 0.1-1.5 parts inositol; 0.03-0.07 parts ceramide NP; 0.8-2.2 parts tranexamic acid; 0.1-1.5 parts glutathione; 1-4 parts jasmine lactic acid bacteria fermentation extract; and 40-60 parts water.

3. The whitening composition according to claim 1, characterized in that: The mass ratio of lecithin to tranexamic acid is 1:

1.

4. The whitening composition according to claim 1, characterized in that: The mass ratio of inositol to glutathione is 1:

1.

5. The whitening composition according to claim 1, characterized in that: The mass ratio of nicotinamide, taurine, and jasmine lactic acid bacteria fermentation extract is 2-5:1.5:

1.

6. The whitening composition according to claim 5, characterized in that: The mass ratio of nicotinamide, taurine, and jasmine lactic acid bacteria fermentation extract is 4:1.5:

1.

7. The whitening composition according to claim 1, characterized in that: The product comprises the following components by weight: 8 parts nicotinamide; 3 parts taurine; 1.8 parts lecithin; 0.8 parts inositol; 0.05 parts ceramide NP; 1.8 parts tranexamic acid; 0.8 parts glutathione; 2 parts jasmine lactic acid bacteria fermentation extract; and 40 parts water.

8. A method for preparing the whitening composition according to any one of claims 1-7, characterized in that: Includes the following steps: First, add niacinamide, taurine, lecithin, inositol, ceramide NP, tranexamic acid and glutathione to water, mix well, then add jasmine lactic acid bacteria fermentation extract and continue mixing until uniform to obtain the whitening composition.

9. The use of the whitening composition according to any one of claims 1-7 in the preparation of cosmetics with whitening effects.

10. A cosmetic product with whitening effect, characterized in that: The cosmetic product comprises the whitening composition according to any one of claims 1-7 and cosmetic excipients acceptable thereto; the excipients are selected from one or more of fillers, emulsifiers, emollients, pH adjusters, suspending agents, stabilizers, and moisturizers; the cosmetic product is a spray.

Images