Acne mask and preparation process thereof

Abstract

The invention discloses an acne mask and a preparation process thereof, and belongs to the field of dressings, the acne mask comprises 5-15 g of magnolia flower, 10-20 g of bighead atractylodes rhizome, 10-20 g of honeysuckle, 10-20 g of bletilla striata, 10-20 g of scutellaria baicalensis, 10-20 g of dandelion, 5-10 g of asarum, 5-10 g of mint, 10-20 g of rheum officinale, 10-20 g of golden cypress, 10-20 g of coptis chinensis, 10-20 g of fructus forsythiae, 10-20 g of radix angelicae and 3-8 g of borneol. The problems that existing western medicines have large irritation to skin mucous membranes, skin dryness and water shortage are easily caused, and skin flora distribution is disordered are solved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of dressings, and particularly relates to a facial mask for acne and a preparation process thereof. BACKGROUND

[0002] Chinese medicine's understanding of the causes and pathogenesis of acne

[0003] The earliest record of acne in Chinese medicine appeared in Huangdi Neijing·Suwen, which believed that the onset of acne was related to internal dampness-heat and external wind-cold. In the Ming Dynasty, Gong Yanxian in Wanbing Huichun and Shen Douyuan in Waigong Qiyuan mentioned that the onset of acne was related to wind-heat of lung meridian and fire-heat of upper energizer. Traditional Chinese medicine believes that the causes and pathogenesis of acne are mainly external wind, wind-heat of lung meridian, liver and kidney yin deficiency, internal dampness-heat, yin-yang imbalance, and disorder of the uterus and conception vessel.

[0004] Long-term stay in a humid and high-temperature environment, often eat spicy and stimulating food, and high mental stress can all cause the spleen and stomach to fail to function normally, cause internal dampness-heat, and finally cause acne. The occurrence of acne is also closely related to the lungs. In the Sui Dynasty, Niao Yuanfang pointed out in Zhushi Yunchuanlun that "wind" and "heat" were one of the causes of acne. In the Ming Dynasty, Chen Shigong believed in Waike Zhengzong that the cause of acne was lung and spleen heat. If liver stagnation and kidney deficiency persist for a long time, the body's tianqi metabolism will be disorderly, which can also lead to phlegm and blood stasis type acne.

[0005] Western medicine's understanding of the causes and pathogenesis of acne

[0006] The sebaceous gland is the site of acne, and excessive keratinization of the sebaceous gland can make the duct diameter smaller, increase the secretion of sebaceous gland cells, and cause a large amount of sebum to accumulate in the pore, causing blockage and leading to acne. The secretion of androgens is also related to the onset of acne. An increase in androgen secretion can promote sebaceous gland secretion and lead to the occurrence of acne.

[0007] The abnormal growth of bacterial flora on the human skin can also lead to the occurrence of acne. Current research has found that the bacteria associated with the onset of acne include Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. When Propionibacterium acnes proliferates in large numbers, it can produce a series of inflammatory reactions, promote sebaceous gland secretion, and trigger a series of diseases such as sebaceous gland duct hyperplasia, ultimately leading to the occurrence of acne.

[0008] Acne is also related to environmental factors, mental health, and unhealthy lifestyle habits. Excessive psychological and emotional stress increases the likelihood of developing acne, and those with acne are more susceptible to emotional distress; the longer the condition persists, the greater the emotional impact. Prolonged exposure to the face from electronic screens such as mobile phones and computers can cause hormonal imbalances and increased sebum secretion, leading to or worsening acne. Over-cleansing the face can damage the skin's barrier function, also triggering acne. An unhealthy diet can also contribute to acne; long-term consumption of dairy products, high-sugar, high-salt, and spicy foods can induce acne.

[0009] Traditional Chinese Medicine Treatment for Acne

[0010] smear

[0011] Topical application methods mainly include traditional Chinese medicine creams, ointments, tinctures, and powders. Zhao Zhanzhou et al. used a self-made acne ointment for external application to treat mild to moderate acne. Chen Jiemin used a sterile three-edged needle combined with a Sanhuang tincture to treat acne. The preparation method of the Sanhuang tincture is as follows: Mix 1g of Scutellaria baicalensis powder, 19g of rhubarb powder, 1g of Coptis chinensis powder, and 1g of pollen with 75% alcohol until homogeneous.

[0012] Fumigation

[0013] Fumigation therapy involves boiling Chinese herbs in water or heating them in other ways, and then applying the steam generated after boiling directly to the affected skin. This method accelerates skin metabolism by heating the affected skin, effectively softening keratinized hair follicles, and allowing the therapeutic components of the Chinese herbs to be more easily absorbed by the skin, thereby achieving the goal of treating acne. Mei Lihong et al. used self-selected Chinese herbs to perform fumigation therapy on acne patients (a mixture of Phellodendron bark, rhubarb, and mulberry bark soaked in warm water for 30 minutes, boiled twice, with the first boiled over high heat and the second over low heat, and the second boiled over high heat and low heat for 10 minutes each).

[0014] External application

[0015] External application of traditional Chinese medicine, also known as herbal plaster application, involves pulverizing Chinese medicinal herbs, mixing them with distilled water, honey, or alcohol, or using other methods to create a paste. After sterilization, this paste is applied to the face using a facial mask sheet or directly to the patient's face. The advantage of direct application is that it allows the medicinal components to directly contact the skin and be absorbed, exerting their effects directly in the hair follicles. This promotes increased moisture content in the stratum corneum and the absorption of active ingredients, increasing the absorption rate and reducing drug loss during absorption and metabolism.

[0016] External washing method of traditional Chinese medicine

[0017] Traditional Chinese medicine external washing involves using a medicinal liquid made from decocting or soaking Chinese herbs to cleanse the affected skin. Through the warming effect of the medicinal liquid and transdermal absorption, the medicinal herbs exert their effects, thereby improving skin condition, reducing inflammation and pain, and relieving swelling and itching to treat acne.

[0018] Western medicine treatments for acne

[0019] Organic acid drugs

[0020] Among organic acid medications, fruit acids are commonly used to treat acne. Also known as alpha-hydroxy acids, fruit acids have a simple structure, and most are natural organic acids extracted from fruits. They are safe, non-toxic, and have a small molecular weight, allowing them to penetrate well into the dermis, accelerate the epidermal cell renewal cycle, inhibit the growth and reproduction of Propionibacterium acnes, alleviate skin inflammation, regulate sebaceous gland secretion, and promote the regeneration and synthesis of collagen. This effectively reduces acne lesions and speeds up the skin repair process, making them highly effective in treating acne.

[0021] Retinoids

[0022] Retinoic acid, a derivative of vitamin A, can inhibit melanin production, reduce acne scars, and inhibit granulocyte chemotaxis, thus exerting an anti-inflammatory effect. It can also regulate the differentiation of epithelial cells in the pilosebaceous duct, reduce intercellular adhesion, dissolve microcomedones and comedones, and prevent their formation. Once comedones are dissolved, the anaerobic environment for Propionibacterium acnes to survive is disrupted, reducing the production of inflammatory lesions such as papules and pustules. However, these medications can irritate the skin, causing dryness, peeling, tightness, and localized erythema.

[0023] Antibacterial drugs

[0024] A benzoyl peroxide drug

[0025] Benzoyl peroxide is an oxidizing agent. When applied topically to acne-prone skin, it releases nascent oxygen, which can kill Propionibacterium acnes and reduce free fatty acids in the epidermis. It is commonly used to treat mild to moderate acne and can also be used in combination with other medications to treat severe acne. However, benzoyl peroxide can also irritate the skin, causing dryness, itching, discoloration, and damage to the skin barrier.

[0026] Class B antibiotics

[0027] Antibiotics come in both oral and topical forms. Oral antibiotics include macrolides and dimethylamine tetracyclines, while topical antibiotics include clindamycin, fusidic acid, and chloramphenicol. Topical antibiotics are generally suitable for patients with mild to moderate acne, while oral antibiotics are suitable for treating moderate to severe acne. Antibiotics can reduce the number of inflammatory lesions and have the advantages of rapid onset of action, minimal irritation, and short treatment courses. However, they are not suitable for long-term use and can lead to drug resistance in Propionibacterium acnes.

[0028] C hormone drugs

[0029] In treating acne, steroid hormones such as dexamethasone and androgen blockers such as spironolactone and tanshinone are commonly used. These hormonal drugs reduce the synthesis of androgen precursors and inhibit androgen activity, thereby reducing sebum secretion and treating acne. However, long-term use of hormonal drugs can produce serious side effects. Dexamethasone is a glucocorticoid; its use by pregnant women increases the risk of miscarriage.

[0030] Physical therapy

[0031] Physical therapy primarily plays an adjunctive role in the treatment of acne. This method is effective quickly and has few side effects. Physical therapies include laser therapy, photodynamic therapy, radiofrequency therapy, and intense pulsed light (IPL).

[0032] Comprehensive therapy

[0033] Comprehensive therapy combines topical Chinese medicine with modern optics, oral medications, nanotechnology, acupuncture, acids such as salicylic acid, fruit acids, and IPL instruments to treat acne.

[0034] Long-term use of Western medicine to treat acne can easily lead to drug resistance and cause endocrine disorders. In contrast, traditional Chinese medicine is effective in relieving clinical symptoms and controlling the condition of acne, and has the advantages of fewer side effects, less recurrence, and less skin irritation.

[0035] Currently, there are two Chinese patents (CN103751055A and CN107281029A). One is an acne mask powder that uses only raw medicinal powder, resulting in low bioavailability of the medicinal materials. The other uses water-extracted Chinese herbal extracts, but the effects are not significant and need further improvement.

[0036] This patent is based on the clinical application of traditional Chinese medicine facial masks in the treatment of acne. It conducts research on the preparation process of traditional Chinese medicine acne facial masks, uses the Oxford cup method to determine the antibacterial effect of Staphylococcus aureus and Staphylococcus epidermidis in the production of the masks, and selects the top three masks with the best overall antibacterial effect by the diameter of the inhibition ring. Then, it studies the antibacterial effect of Propionibacterium acnes, conducts skin and mucous membrane irritation experiments on rabbits, and also selects volunteers for human trials.

[0037] Previously, Chinese patent CN103751055A disclosed an acne mask. Its effective Chinese herbal ingredients include a Chinese herbal extract at a ratio of 0.2-2g of medicinal material / mL, which is extracted by decoction of the following Chinese herbs in the indicated weight ratios: Scutellaria baicalensis 15-30 parts, Coptis chinensis 25-35 parts, Phellodendron chinense 30-40 parts, Taraxacum mongolicum 20-30 parts, Angelica sinensis 10-15 parts, Lonicera japonica 15-25 parts, Forsythia suspensa 5-15 parts, and Houttuynia cordata 0-10 parts. Each mask contains 80mL of the herbal extract. Propylene glycol, carrageenan, and ethylparaben are added. The ethylparaben is heated to 80-90℃ and stirred thoroughly for 15 minutes. The pH is adjusted to 5.5-6.5, cooled to about 50℃, and 0.5% hyaluronic acid and collagen are added. The mixture is stirred until completely dissolved, formed into a film, packaged, and sterilized. It has the effect of removing oil and treating acne, but the effect of this patent is not significant and needs to be improved.

[0038] Chinese patent CN107281029A discloses an acne mask powder, mainly composed of 260-280 parts rhubarb, 260-280 parts turmeric, 260-280 parts phellodendron bark, 170-190 parts bletilla rhizome, 120-140 parts angelica root, 120-140 parts red peony root, 120-140 parts trichosanthes root, 120-140 parts indigo naturalis, 120-140 parts licorice root, and 120-140 parts vegetable and fruit enzyme powder. The herbs are dried, dust-removed, and then pulverized into a powder of at least 200 mesh. All raw materials are pure traditional Chinese medicine, the preparation process is simple, and there are no chemical additives. It has the effects of clearing heat and detoxifying, anti-inflammatory and insecticidal, cooling blood and promoting blood circulation, reducing swelling and removing acne, and is used for nodular, cystic, and mixed acne. However, this patented mask powder is a raw herb powder, and its bioavailability is not high, requiring further improvement.

[0039] Turmeric: It breaks up blood stasis, promotes qi circulation, regulates menstruation, and relieves pain. It is used to treat abdominal distension and pain, arm pain, abdominal masses, amenorrhea due to blood stasis in women, postpartum abdominal pain due to blood stasis, injuries from falls, and carbuncles.

[0040] Phellodendron bark: Clears heat, dries dampness, drains fire, and detoxifies. Treats dysentery, diarrhea, thirst, jaundice, weakness, nocturnal emission, urinary tract infections, hemorrhoids, hematochezia, leukorrhea, steaming bone fever, red and swollen eyes, sores in the mouth and tongue, and boils and carbuncles.

[0041] Bletilla striata: Rich in starch, glucose, volatile oil, mucilage, etc., it can be applied topically to eliminate acne scars on the face.

[0042] It leaves skin smooth and flawless. The bulb contains Bletilla striata gum, starch, volatile oil, etc., which have the effects of reducing swelling and stopping bleeding, and whitening the skin. It can be applied externally to treat traumatic bleeding, carbuncles, burns, boils, etc.

[0043] Angelica dahurica: dispels wind and dampness, promotes blood circulation and drains pus, promotes tissue regeneration and relieves pain. Used for headache, toothache, sinusitis, hemorrhoids, leukorrhea, carbuncles and sores, and itchy skin.

[0044] Honeysuckle: The dried flower buds or unopened flowers of *Lonicera japonica*, a plant belonging to the genus *Lonicera* in the family Caprifoliaceae. It has the effect of clearing heat and detoxifying.

[0045] Dandelion: A herbaceous plant belonging to the Asteraceae family, the whole plant is used medicinally. It has the effects of reducing swelling and lumps, soothing the liver and relieving depression, clearing heat and detoxifying, and removing dampness and reducing swelling.

[0046] Forsythia: A shrub belonging to the genus Forsythia in the family Oleaceae. Its fruit is used medicinally. It has the effects of reducing swelling and lumps, and clearing heat and detoxifying.

[0047] Scutellaria baicalensis: A perennial herbaceous plant belonging to the Lamiaceae family, its root is used medicinally. It has the effects of clearing heat and drying dampness, purging fire and detoxifying, and stopping bleeding.

[0048] Asarum: Aristolochiaceae family, the dried whole herb is used medicinally, and has the effects of dispelling wind and cold, clearing the orifices and relieving pain, warming the lungs and resolving phlegm.

[0049] In addition, there is rhubarb, which has the effects of clearing heat and purging fire, cooling blood and detoxifying, and purging accumulations; and phellodendron bark, which has the effects of clearing heat and drying dampness, purging fire and eliminating steaming, and detoxifying and healing sores.

[0050] Currently, acne treatment in China mostly relies on Western medicines such as antibiotics and all-trans retinoic acid. While these Western medicines can quickly control the inflammatory response of acne, they are also highly irritating to the skin and mucous membranes, easily causing dryness, dehydration, and imbalances in the skin's microbiome. Summary of the Invention

[0051] Purpose of the invention: The purpose of this invention is to provide an acne mask and its preparation process, in order to solve the problems of existing Western medicines being highly irritating to the skin and mucous membranes, easily causing dryness and dehydration of the skin, and causing skin flora imbalance.

[0052] To achieve the above objectives, the present invention provides the following technical solution: an acne mask, characterized in that the formula includes 5-15g of Magnolia biondii, 10-20g of Atractylodes macrocephala, 10-20g of Lonicera japonica, 10-20g of Bletilla striata, 10-20g of Scutellaria baicalensis, 10-20g of Taraxacum mongolicum, 5-10g of Asarum heterotropoides, 5-10g of Mentha haplocalyx, 10-20g of Rheum palmatum, 10-20g of Phellodendron chinense, 10-20g of Coptis chinensis, 10-20g of Forsythia suspensa, 10-20g of Angelica dahurica, and 3-8g of Borneol.

[0053] The preferred ingredients are: Magnolia biondii 10g, Atractylodes macrocephala 15g, Lonicera japonica 15g, Bletilla striata 15g, Scutellaria baicalensis 15g, Taraxacum mongolicum 15g, Asarum heterotropoides 5g, Mentha haplocalyx 5g, Rheum palmatum 15g, Phellodendron chinense 15g, Coptis chinensis 15g, Forsythia suspensa 15g, Angelica dahurica 15g, and Borneol 5g.

[0054] A process for preparing an acne mask, characterized in that:

[0055] Step 1: Add 6 times the volume of 50% ethanol to the above raw material formula, soak for 24 hours, filter, and concentrate the filtrate to 100mL;

[0056] Step 2: Add 5 mL of distilled water to a 25 mL beaker, boil for 5 minutes, and after returning to room temperature, add 3.5 g of precisely weighed glycerin and stir until the glycerin dissolves.

[0057] Step 3: Add 5mL of distilled water, 1mL of sweet orange essential oil, and 3mL of filtrate to a 25mL beaker, and stir until dissolved;

[0058] Step 4: Mix the two liquids from Step 2 and Step 3 thoroughly to obtain the mask liquid. After preparation, sterilize the mask under a sterilizing lamp for 1-3 hours, then refrigerate it at 4°C. The glycerin concentration is 0.7g / mL.

[0059] Compared with the prior art, the beneficial effects of the present invention are:

[0060] The main purpose of this invention is to prepare various traditional Chinese medicine acne masks through different extraction methods and formulations, and to study their potential antibacterial and anti-acne effects.

[0061] The materials used in the experiment were mostly common and readily available. The preparation process of the face mask was relatively simple, requiring no complex equipment or specialized techniques. The simple operation reduced errors and uncertainties in the preparation process, improving the quality and stability of the face mask.

[0062] Some of the Chinese medicinal herbs and essential oils used in the experiment typically have natural, refreshing scents that can improve the aroma of the face mask. Examples include the delicate fragrance of honeysuckle and the coolness of mint. These scents not only bring pleasure but also have a cooling, calming, and relaxing effect.

[0063] The glycerin and other substances used in the experiment can give the mask good lubrication and moisturizing properties, allowing it to adhere to the skin during use, lock in moisture and nutrients on the skin surface, and prevent external environmental irritation and pollution.

[0064] Refrigerating facial mask liquid can extend its shelf life, prevent the growth and contamination of microorganisms, and maintain the stability and effectiveness of the active ingredients. Using a sterilizing lamp can eliminate microbial contamination in the mask liquid, ensuring the safety and hygiene of the mask.

[0065] This patent uses a combination extraction technology and extracts from traditional Chinese medicine decoctions and ethanol soaking solutions to make a facial mask liquid. The three have different antibacterial effects against Staphylococcus aureus and Staphylococcus epidermidis, with the mask made from the ethanol soaking solution being more effective. Attached Figure Description

[0066] Figure 1 This is a flowchart of the present invention. Detailed Implementation

[0067] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0068] Example

[0069] The formula (Magnolia biondii 10g, Atractylodes macrocephala 15g, Lonicera japonica 15g, Bletilla striata 15g, Scutellaria baicalensis 15g, Taraxacum mongolicum 15g, Asarum heterotropoides 5g, Mentha haplocalyx 5g, Rheum palmatum 15g, Phellodendron chinense 15g, Coptis chinensis 15g, Forsythia suspensa 15g, Angelica dahurica 15g, Borneol 5g) is soaked in 6 times its volume of 50% ethanol for 24 hours, then filtered. The filtrate is concentrated to 100mL. 3mL of the extract is taken, and 5mL of 0.7g / mL glycerin, 5mL of distilled water, and 1mL of sweet orange essential oil are added. The resulting mask has the best antibacterial effect against three acne-causing bacteria (Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes). Rabbit skin irritation experiments have proven that this patented mask is non-irritating, and human trials have shown significant results.

[0070] Mask formula selection

[0071] This experiment used various Chinese medicinal herb combinations and folk remedies, employing different extraction methods to prepare different Chinese herbal face masks. The formulas are shown in Table 1.

[0072]

[0073] Table 1. Traditional Chinese Medicine Facial Mask Formulas

[0074] 4.3.2 Extraction of medicinal materials

[0075] Accurately weigh the herbal powders from Formulas 1, 2, and 3 and add them separately to an extraction machine. Add six times the volume of distilled water for extraction. Filter the condensate and add the filtrate to a round-bottom flask. Concentrate the filtrate to half its volume using a rotary evaporator at 75°C. REF _Ref19698 \n \h

[16] After obtaining the extract and returning it to room temperature, label it in sequence as "1, 2, 3" and refrigerate it.

[0076] Accurately weigh the mixed Chinese herbal powders from Formula 1, Formula 2, and Formula 3 and add them separately to beakers. Then add six times the volume of 50% ethanol to the flask. REF _Ref22520 \n \h

[17] After soaking for 24 hours, the solution was filtered to obtain the filtrate. The filtrate was added to a round-bottom flask and concentrated to half its volume at 75°C using a rotary evaporator to obtain the extract. After returning to room temperature, the extract was labeled in sequence as "4, 5, 6" and then refrigerated.

[0077] Accurately weigh the mixed Chinese herbal powders from Formulas 1, 2, and 3, and add them to a 1 L round-bottom flask. Add six times the volume of distilled water to the flask, and decoct at 80°C for 60 minutes. Repeat this process twice. Filter the condensate, combine the filtrates, and concentrate them to 1 / 4 volume using a rotary evaporator to obtain the extract. After restoring to room temperature, label them sequentially as "7, 8, 9". Refrigerate.

[0078] Accurately weigh out the amount from formula 4, add 50 mL of water to dissolve it, filter, and label it "10". Refrigerate.

[0079] Accurately weigh out Formula 5, add 50 mL of distilled water, soak for 24 hours, filter, and label the filtrate with "11". Refrigerate.

[0080] Accurately weigh out Formula 6, filter to obtain 50 mL of filtrate, and label it "12". Refrigerate.

[0081] Accurately weigh out the amount from formula 7, filter it to obtain 50 mL of filtrate, and label it "13". Refrigerate it.

[0082] 4.3.3 Preparation of Facial Mask

[0083] Add 5 mL of distilled water to a 25 mL beaker, boil for 5 minutes, and after returning to room temperature, add 3.5 g of precisely weighed glycerol and stir until the glycerol dissolves.

[0084] Add 5 mL of distilled water, 1 mL of sweet orange essential oil, and 3 mL of the "1" extract prepared earlier to a 25 mL beaker, and stir until dissolved.

[0085] Mix the two liquids from (1) and (2) thoroughly to obtain the No. 1 facial mask liquid. After preparation, sterilize the mask under a sterilizing lamp for 1-3 hours, then refrigerate it at 4°C.

[0086] Prepare mask liquids No. 2 to No. 13 according to steps (1), (2) and (3), and refrigerate at 4°C.

[0087] 4.3.4 Screening of antibacterial effects of face masks

[0088] The antibacterial effect of masks No. 1 to No. 13 was determined by the Oxford cup method, and the top three masks with the best overall antibacterial effect were selected by the diameter of the inhibition ring.

[0089] Experimental Operation

[0090] The specific experimental steps are as follows: Sterilized LB agar medium was poured into plates and allowed to cool and solidify. Then, 100 μL of Staphylococcus aureus and Staphylococcus epidermidis suspensions were added to the medium and spread evenly using a spreader. Each medium was placed in an Oxford cup, and 200 μL of the negative control group (a facial mask solution using water instead of the medication), the positive control group (DouMeiYan Acne Treatment Cream), and 1–13 μL of the facial mask solution were added respectively. Staphylococcus aureus and Staphylococcus epidermidis were incubated at 37°C for 24 hours, and the diameter of the inhibition zone was measured. Three experiments were conducted, and the data were collected.

[0091] 4.3.4.1 Results of Staphylococcus aureus antibacterial experiment

[0092]

[0093] Table 2. Diameter of the inhibition zone of Staphylococcus aureus (mm)

[0094] As shown in Table 2 (REF _Ref20936 \h), among the 13 types of face masks, the top five masks in terms of their inhibitory effect on the growth and reproduction of Staphylococcus aureus are:

[0095] Mask No. 2 (12.26±0.22mm)

[0096] Mask No. 4 (10.33±0.23mm)

[0097] Mask No. 5 (10.33±0.47mm)

[0098] Mask No. 1 (9.17±0.23mm)

[0099] Mask No. 6 (8.66±0.23mm)

[0100] Since the average diameter of the antibacterial rings of masks #4 and #5 is the same, we can consider their RSD to determine which one is more stable. Since mask #4 has a smaller RSD, we believe that the antibacterial effect of mask #4 is more stable.

[0101] 4.3.4.2 Results of Staphylococcus epidermidis antibacterial test

[0102] The results of the experiment on the inhibition of Staphylococcus epidermidis by 13 kinds of facial masks are as follows:

[0103]

[0104] Table 3. Diameter of the inhibition zone of Staphylococcus epidermidis (mm)

[0105] According to the data in Table 3 (REF _Ref4081 \h), mask #2 showed the largest average diameter of the inhibition zone against Staphylococcus epidermidis, indicating a better antibacterial effect. Masks #5, #4, #7, and #6 followed. Therefore, the top five masks in terms of effectiveness against the growth and reproduction of Staphylococcus epidermidis are:

[0106] Mask No. 2 (10.60±0.29mm)

[0107] Mask No. 5 (9.40±0.29mm)

[0108] Mask No. 4 (9.06±0.09mm)

[0109] Mask No. 7 (8.53±0.04mm)

[0110] Mask No. 6 (8.40±0.29mm)

[0111] These face masks are effective in inhibiting the growth and reproduction of Staphylococcus epidermidis.

[0112] 4.3.4.3 Optimal Concentration Screening

[0113] Based on the antibacterial test results of the above 13 types of facial masks against Staphylococcus aureus and Staphylococcus epidermidis, the following conclusions can be drawn: Mask No. 2 showed the best effect in inhibiting the growth and reproduction of both Staphylococcus aureus and Staphylococcus epidermidis, making it the mask with the best overall antibacterial effect. Furthermore, masks No. 4 and No. 5 ranked among the top three in average diameter of inhibition zones in the antibacterial experiments against both pathogens, indicating that these two masks also possess good antibacterial properties. In conclusion, masks No. 2, No. 4, and No. 5 were selected for their respective optimal concentrations.

[0114] The antibacterial effects of masks No. 2, No. 4, and No. 5 on Staphylococcus aureus and Staphylococcus epidermidis were tested at different concentrations (positive control group, 5 times concentration, 10 times concentration, 1 times concentration of traditional Chinese medicine extract, and pure traditional Chinese medicine extract).

[0115] Experimental Operation

[0116] The specific experimental steps are as follows: Pour the sterilized agar medium into plates, allow it to cool and solidify, then add 100 μl of Staphylococcus aureus and Staphylococcus epidermidis bacterial suspensions to the medium and spread evenly. Place 5 Oxford cups in each medium and label them as follows:

[0117] A: Add 200ul of the positive control group (Doubenyan Acne Cream).

[0118] B: Add 200ul of the original concentration of the facial mask liquid (1x concentration).

[0119] C: Add 200ul of extract mask liquid (5 times concentration, obtained by concentrating the Chinese herbal extract 5 times).

[0120] D: Add 200ul of extract mask liquid (10 times concentration, obtained by concentrating the Chinese herbal extract 10 times).

[0121] E: Add 200ul of pure Chinese herbal extract (all Chinese herbal extract, no other liquids from the mask are added).

[0122] The antibacterial effects of different concentrations of masks No. 2, No. 4, and No. 5 against Staphylococcus aureus and Staphylococcus epidermidis are shown in the following figures:

[0123] The antibacterial data of the face mask against Staphylococcus epidermidis and Staphylococcus aureus are as follows:

[0124] Table 4. Diameter of the antibacterial zone of traditional Chinese medicine facial masks (mm) (N=3)

[0125]

[0126] After performing a t-test on the positive control data and other sample data, the result was P = 0.89 > 0.05. This indicates that there was no significant difference in the antibacterial efficacy against Staphylococcus aureus and Staphylococcus epidermidis between the positive control group and other samples.

[0127] Statistical data reveals that:

[0128] Mask No. 2: With increasing concentration, the antibacterial effect against Staphylococcus aureus increased slightly, but the increase was not significant. The antibacterial effect against Staphylococcus epidermidis increased slightly at a 5x concentration, but remained unchanged at a 10x concentration.

[0129] Mask No. 4: As the concentration increases, the antibacterial effect against Staphylococcus aureus decreases slightly, but the antibacterial effect against Staphylococcus epidermidis remains unchanged at 5 times the concentration, and then decreases at 10 times the concentration. Mask No. 4 at its original concentration has the best antibacterial effect against Staphylococcus aureus.

[0130] Mask No. 5: As the concentration increases, the antibacterial effect against Staphylococcus aureus decreases slightly, but the antibacterial effect against Staphylococcus epidermidis increases significantly at both 5x and 10x concentrations. Mask No. 5 at 5x concentration shows the best antibacterial effect against Staphylococcus epidermidis.

[0131] Based on the above analysis, we can draw the following conclusions: For Staphylococcus aureus, Mask No. 4 (original concentration) showed the best antibacterial effect. For Staphylococcus epidermidis, Mask No. 5 (5x concentration) showed the best antibacterial effect. Although Mask No. 2 did not show the best antibacterial effect at 10x concentration, considering its stability at different concentrations, it can still be considered. Therefore, the three samples ultimately selected for the Propionibacterium acnes antibacterial effect experiment were: Mask No. 4 (original concentration), Mask No. 5 (5x concentration), and Mask No. 2 (10x concentration). These samples all showed good effects in inhibiting Staphylococcus aureus and Staphylococcus epidermidis.

[0132] Antibacterial activity test of Propionibacterium acnes

[0133] Propionibacterium acnes is one of the main pathogens causing acne (pimples, blackheads, etc.). Antibacterial experiments against Propionibacterium acnes can be used to evaluate the effectiveness of face masks in inhibiting the growth of this bacterium and analyze their anti-acne effects.

[0134] Pour the sterilized brain and heart extract culture medium into plates. After cooling and solidification, add 100 μl of Propionibacterium acnes suspension to the medium and spread it evenly with a spreader. Place 5 Oxford cups in each culture medium and label them accordingly.

[0135] a: Add 200ul of positive control group (Doubenyan Acne Cream)

[0136] b: Add 200ul of No. 4 face mask (1x concentration)

[0137] c: Add 200ul of No. 5 face mask (5 times concentration)

[0138] d: Add 200ul of No. 2 face mask (10 times concentration)

[0139] e: Add 200 μL of negative control group (using water instead of the herbal extract).

[0140] Propionibacterium acnes was cultured at 37°C for 48 hours. The diameter of the inhibition zone was measured. Two more replicate experiments were performed. Statistical data were collected to analyze the antibacterial effect.

[0141] The experimental data on the inhibitory effect of the face mask on Propionibacterium acnes are shown in the table below:

[0142]

[0143] Table 5. Diameter of the antibacterial zone (mm) of traditional Chinese medicine facial masks

[0144] After performing t-tests on the positive control group and mask groups 5, 2, and 4, the p-value for mask group 5 was ≥0.05, indicating no significant difference, and its antibacterial effect was comparable to that of the positive control group. However, after comparing masks 2 and 4 with the positive control group, the p-value was ≤0.05, indicating significant differences, and their antibacterial effects differed greatly from those of the positive control group.

[0145] After performing a t-test between the negative control group and each sample group, the result was P≤0.05. This means that compared with the negative control group, each sample group showed an inhibitory effect on Propionibacterium acnes.

[0146] In summary, the positive control group showed the best antibacterial effect, with a larger inhibition zone diameter. Mask No. 2 (10x concentration), Mask No. 4 (original concentration), and Mask No. 5 (5x concentration) also showed some antibacterial effects, with Mask No. 5 (5x concentration) showing an antibacterial effect closer to that of the positive control group. Therefore, Mask No. 5 (5x concentration) exhibits good inhibitory effects on the growth and reproduction of Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes, and can be considered the optimal choice.

[0147] Summary of antibacterial test results

[0148] The Oxford Cup antibacterial experiment (Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes) showed that the 13 prepared facial masks had a certain inhibitory effect on the growth and reproduction of Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes. Among the 13 masks, masks No. 2, No. 4, and No. 5 showed the most significant antibacterial effects against Staphylococcus aureus and Staphylococcus epidermidis. When selecting the optimal concentration of the herbal extract after choosing three mask formulas, masks No. 2 (10 times the extract concentration), No. 4 (original concentration), and No. 5 (5 times the extract concentration) showed the most prominent antibacterial effects against Staphylococcus aureus and Staphylococcus epidermidis. After the antibacterial experiment against Propionibacterium acnes, mask No. 5 (5 times the extract concentration) showed the most prominent and stable antibacterial effect against all three acne-causing bacteria.

[0149] Therefore, the preparation method of the face mask is as follows: 10g of Magnolia biondii, 15g of Atractylodes macrocephala, 15g of Lonicera japonica, 15g of Bletilla striata, 15g of Scutellaria baicalensis, 15g of Taraxacum mongolicum, 5g of Asarum heterotropoides, 5g of Mentha haplocalyx, 15g of Rheum palmatum, 15g of Phellodendron chinense, 15g of Coptis chinensis, 15g of Forsythia suspensa, 15g of Angelica dahurica, and 5g of Borneol. Add six times the volume of 50% ethanol, soak for 24 hours, filter, concentrate the filtrate to 100mL, measure 3mL of the extract, add 5mL of 0.7g / mL glycerin, 5mL of distilled water and 1mL of sweet orange essential oil. The prepared face mask has the best antibacterial effect against three acne-causing bacteria (Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes).

[0150] Skin and mucous membrane irritation test

[0151] Experimental reagents and materials: homemade face mask, physiological saline, hair removal agent, 75% alcohol, scissors, dropper, gauze, plastic film, non-irritating adhesive tape and bandages.

[0152] Laboratory animals: Six rabbits with intact skin were used in each embodiment;

[0153] Specific experimental methods

[0154] 24 hours before the experiment, use a hair removal agent to remove the hair on both sides of the spine on the back of the rabbit, taking care not to damage the skin. The area to be hair removed should be approximately 3cm x 3cm on each side.

[0155] Apply 0.5 ml of the mask of this invention directly to the skin, then cover it with two layers of gauze (2.5 cm × 2.5 cm) and a layer of plastic film, and then secure it with non-irritating adhesive tape and bandage. The other side of the skin serves as a control. A closed-cell test was conducted, with the application time being 2 hours. Skin reactions such as erythema, eschar, and edema were observed at the application site at 1, 24, 48, and 72 hours after application.

[0156] Results evaluation: At each observation time point, the formation of skin erythema and edema in the animals was scored according to Table 6. The scores of the six animals were added together at each time point (1, 24, 48, 72h) and divided by the number of animals to obtain the average skin irritation response score (irritation index) at different time points.

[0157]

[0158] Table 6. Scoring criteria for skin irritation reaction

[0159]

[0160] Table 7 Skin Irritation Intensity Grading

[0161] Experimental results:

[0162] Skin lesion test results: The total score of lesions in the 6 rabbits at 1h, 24h, 48h, and 72h was 0, and the stimulation index was less than 0.5cm. 2 It is a non-irritating product.

[0163] Evaluation of the therapeutic effect of facial masks

[0164] Experimental objective: To evaluate the efficacy of the present invention by using a self-made facial mask as a control group and Doubenyan acne cream as a control group.

[0165] Experimental subjects: Forty acne patients who had not used any acne removal products within 4 weeks and voluntarily participated in the cooperation were selected. Among them, 20 patients with grade III acne were divided into 2 groups; and 20 patients with grade IV acne were divided into 2 groups.

[0166] Observation period: Observations were conducted in the first, third, and sixth weeks respectively.

[0167] Detection indicators: total number of skin lesions, transepidermal water loss, skin moisture content, sebum content, and skin surface pH.

[0168] Evaluation criteria:

[0169]

[0170] Total number of skin lesions = Factor score × Skin lesion score

[0171]

[0172] Overall therapeutic effect index = (Total number of skin lesions before treatment - Total number of skin lesions after treatment) / Total number of skin lesions before treatment × 100%

[0173] Evaluation of therapeutic effect in Example 1

[0174] Twenty patients with grade III acne were included in the study. Ten patients used Doubenyan Acne Removal Cream as the control group, and ten patients used the mask of this invention as the sample group. The male and female groups were evenly divided. The mask was applied three times a week, and the efficacy was observed at the first, third, and sixth weeks after use.

[0175]

[0176] Therapeutic effects in week six:

[0177]

[0178] The data above shows that patients experienced some improvement in their symptoms after using Doubenyan Acne Cream, but the cure rate was very low. After using the mask of this invention, patients experienced significant symptom relief, and the cure rate increased significantly.

[0179] Evaluation of therapeutic effect in Example 2

[0180] Twenty patients with grade IV acne were divided into two groups: 10 patients used Doubenyan Acne Removal Cream as the control group, and 10 patients used the mask of this invention as the sample group. The male and female groups were evenly divided. The mask was applied three times a week, and the efficacy was observed at the first, third, and sixth weeks after use.

[0181]

[0182] Therapeutic effects in week six:

[0183]

[0184] The data above shows that patients experienced some improvement in their symptoms after using Doubenyan Acne Cream, but it did not achieve a cure. However, after using the mask of this invention, patients experienced significant symptom relief, achieving a cure.

[0185] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An acne mask, characterized in that: The formula includes 5-15g of Magnolia biondii, 10-20g of Atractylodes macrocephala, 10-20g of Lonicera japonica, 10-20g of Bletilla striata, 10-20g of Scutellaria baicalensis, 10-20g of Taraxacum mongolicum, 5-10g of Asarum heterotropoides, 5-10g of Mentha haplocalyx, 10-20g of Rheum palmatum, 10-20g of Phellodendron chinense, 10-20g of Coptis chinensis, 10-20g of Forsythia suspensa, 10-20g of Angelica dahurica, and 3-8g of Borneol.

2. The acne mask according to claim 1, characterized in that: The formula includes; Magnolia flower 10g, Atractylodes macrocephala 15g, Lonicera japonica 15g, Bletilla striata 15g, Scutellaria baicalensis 15g, Taraxacum mongolicum 15g, Asarum heterotropoides 5g, Mentha haplocalyx 5g, Rheum palmatum 15g, Phellodendron chinense 15g, Coptis chinensis 15g, Forsythia suspensa 15g, Angelica dahurica 15g, and Borneol 5g.

3. The acne mask preparation process according to claim 1 or 2, characterized in that: Step 1: Add 6 times the volume of 50% ethanol to the above raw material formula, soak for 24 hours, filter, and concentrate the filtrate to 100mL; Step 2: Add 5 mL of distilled water to a 25 mL beaker, boil for 5 minutes, and after returning to room temperature, add 3.5 g of precisely weighed glycerin and stir until the glycerin dissolves. Step 3: Add 5mL of distilled water, 1mL of sweet orange essential oil, and 3mL of filtrate to a 25mL beaker, and stir until dissolved; Step 4: Mix the two liquids from Step 2 and Step 3 thoroughly to obtain the mask liquid. After preparation, sterilize the mask under a sterilizing lamp for 1-3 hours, then refrigerate it at 4°C.

4. The acne mask preparation process according to claim 3, characterized in that: The glycerol concentration was 0.7 g / mL.

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