Traditional Chinese medicine composition for relieving gouty arthritis as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition for relieving gouty arthritis and a preparation method and application thereof. The traditional Chinese medicine composition for relieving gouty arthritis provided by the invention is prepared from the following raw materials in parts by weight: 10 to 45 parts of triquetrous tadehagi herb, 10 to 45 parts of herba elsholtziae and 10 to 45 parts of lalang grass rhizome. The traditional Chinese medicine composition for relieving gouty arthritis is simple in raw material composition and low in cost, can effectively relieve the joint swelling degree, inflammation and gait conditions of gouty joint rats, improve the levels of TNF-alpha, IL-1beta and IL6 of the rats, reduce the level of inflammation-related cytokines and relieve the inflammatory state of the rats, has a remarkable curative effect on gouty arthritis, and is free of toxic and side effects. The traditional Chinese medicine composition is high in safety, small in side effect, free of obvious liver and kidney function damage, free of obvious influence on growth and development and suitable for long-term taking.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of medicine, and particularly relates to a traditional Chinese medicine composition for relieving gouty arthritis and a preparation method and application thereof. BACKGROUND

[0002] Gouty arthritis is an inflammatory reaction caused by the deposition of urate crystals in the synovial membrane, cartilage and other tissues of joints, and is mainly manifested as joint pain, swelling and fever, which seriously affects the quality of life of patients. At present, the drugs for treating gouty arthritis in clinical practice mainly include non-steroidal anti-inflammatory drugs, colchicine and glucocorticoids, and although these drugs have certain effects in relieving pain and inflammation, they have adverse reactions such as gastrointestinal reactions, liver and kidney function damage and the like. Traditional Chinese medicines have unique advantages in treating gouty arthritis, such as Chinese patent application CN107468955A discloses a traditional Chinese medicine for treating gouty arthritis, which is composed of volatile oil and water extract of Chinese herbal medicines, and the components and mass ratio of the Chinese herbal medicines are as follows: 10 of Huangbai, 10 of Cangzhu, 30 of Tufuling, 10 of Gansu, 15 of Weilingxian, 10 of Chishao, 15 of Huzhang, 10 of Dangui, 8 of Shancigu, 10 of Duhuo, 15 of Dangshen and 10 of Jiudaohuang. However, the raw material composition of the traditional Chinese medicine is complex, and the cost is high. Therefore, it has important clinical significance to develop a traditional Chinese medicine composition with simple raw material composition, low cost, relatively small side effects and high safety and effectiveness for relieving gouty arthritis. SUMMARY

[0003] In view of the deficiencies of the prior art, the purpose of the present application is to provide a traditional Chinese medicine composition for relieving gouty arthritis and a preparation method and application thereof. The traditional Chinese medicine composition for relieving gouty arthritis provided by the present application has simple raw material composition, low cost, can effectively relieve the joint swelling degree, inflammation and gait of gouty arthritis rats, improve the TNF-α, IL-1β and IL6 levels of rats, reduce the levels of inflammation-related cytokines, relieve the inflammation state of rats, has remarkable curative effect on gouty arthritis, is high in safety, small in side effects, has no obvious liver and kidney function damage, and has no obvious influence on growth and development.

[0004] The technical scheme of the present application is as follows:

[0005] A traditional Chinese medicine composition for relieving gouty arthritis, comprising the following raw materials and weight fractions: 10-45 parts of Guchu tea, 10-45 parts of Xiangru, and 10-45 parts of Baimaogen.

[0006] Further, the traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and weight fractions: 11.25-22.5 parts of Guchu tea, 11.25-22.5 parts of Xiangru, and 11.25-22.5 parts of Baimaogen.

[0007] Further, the traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and their weight fractions: 11.25-22.5 parts of Folium Trichosanthis, 11.25-15 parts of Herba Moslae, and 11.25-22.5 parts of Radix Imperatae.

[0008] Further, the traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and their weight fractions: 22.5 parts of Folium Trichosanthis, 15 parts of Herba Moslae, and 15 parts of Radix Imperatae.

[0009] The raw material composition and the dosage are important technical features of the traditional Chinese medicine formula, and are closely related to the function of the product. Selecting appropriate raw material composition and dosage is of great significance to ensure the quality of the product. The present application has found, through a large number of creative experiments, that the appropriate dosage range of Folium Trichosanthis, Herba Moslae and Radix Imperatae for compounding has a good effect of relieving gouty arthritis.

[0010] Folium Trichosanthis is the aboveground part of Trichosanthes cucumerina. It has the effects of clearing heat and resolving toxins, removing dampness and reducing yellow, and eliminating accumulation and killing insects. Through its effects of clearing heat and resolving toxins and removing dampness, it helps to eliminate damp-heat evil from the body and reduce the inflammatory response of the joints.

[0011] Herba Moslae is the dried aboveground part of Mosla chinensis or M. perillae. It has the effects of inducing perspiration to relieve the exterior, eliminating dampness to harmonize the middle, and promoting water excretion to reduce swelling. Its effects of eliminating dampness to harmonize the middle and promoting water excretion help to regulate water metabolism in the body, promote the excretion of uric acid, and relieve the symptoms of gouty arthritis.

[0012] Radix Imperatae is the dried rhizome of Imperata cylindrica. It has the effects of cooling blood to stop bleeding and clearing heat to promote diuresis. Through the effect of clearing heat to promote diuresis, it promotes the excretion of metabolic waste such as uric acid, and reduces joint pain and swelling.

[0013] In the present application, the three traditional Chinese medicines of Folium Trichosanthis, Herba Moslae and Radix Imperatae synergistically exert the effects of clearing heat and resolving toxins, removing dampness and turbidity, promoting water excretion and reducing swelling, thereby achieving the purpose of relieving gouty arthritis.

[0014] Another object of the present application is to provide a preparation method of the traditional Chinese medicine composition for relieving gouty arthritis as described above, comprising the following steps:

[0015] Wash Folium Trichosanthis, Herba Moslae and Radix Imperatae, dry them, add water, the amount of water added is 6-10 times the total weight of Folium Trichosanthis, Herba Moslae and Radix Imperatae, soak for 20-40 minutes, then decoct, filter to obtain filtrate 1, add water to the residue, the amount of water added is 6-10 times the total weight of Folium Trichosanthis, Herba Moslae and Radix Imperatae, decoct, filter to obtain filtrate 2, combine filtrate 1 and filtrate 2, and concentrate to obtain the product.

[0016] Further, the amount of water added is 8 times the total weight of Folium Trichosanthis, Herba Moslae and Radix Imperatae, soak for 30 minutes, then decoct.

[0017] Further, the specific operation of decocting is as follows: boiling with large fire, and then decocting with small fire for 0.5-1.5 hours.

[0018] Further, the specific operation of decocting is as follows: boiling with large fire, and then decocting with small fire for 0.5-1.5 hours.

[0019] Another object of the present application is to provide the use of the above-mentioned traditional Chinese medicine composition for relieving gouty arthritis or the traditional Chinese medicine composition prepared by the preparation method of the traditional Chinese medicine composition for relieving gouty arthritis in the preparation of a medicine for relieving gouty arthritis.

[0020] Another object of the present application is to provide a medicine for relieving gouty arthritis, which comprises the above-mentioned traditional Chinese medicine composition for relieving gouty arthritis and a pharmaceutically acceptable excipient.

[0021] Compared with the prior art, the present application has the following advantages:

[0022] (1) The present application can effectively relieve the joint swelling degree, inflammation and gait of gouty arthritis rats, improve the levels of TNF-α, IL-1β and IL6 of rats, reduce the levels of inflammation-related cytokines, relieve the inflammation state of rats, and has a remarkable effect on gouty arthritis.

[0023] (2) The present application has simple raw material composition, small side effects, no obvious liver and kidney function damage, and no obvious influence on growth and development.

[0024] (3) The preparation method of the traditional Chinese medicine prescription of the present application is simple, easy to operate, low in cost, and has a good market application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 Figure 1 is a graph showing the changes of ankle joints of a gouty arthritis rat model;

[0026] Figure 2 Figure 2 is a comparison graph of ankle joints of the rat model after 72 hours. DETAILED DESCRIPTION

[0027] The present application will be further described below through the description of specific embodiments, but this is not a limitation on the present application. Those skilled in the art can make various modifications or improvements according to the basic idea of the present application, as long as they do not deviate from the basic idea of the present application, and they are within the scope of the present application.

[0028] Example 1, a traditional Chinese medicine composition for relieving gouty arthritis

[0029] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and their weight fractions: 15 parts of Itea yunnanensis, 15 parts of Mosla chinensis Maxim, and 15 parts of Imperata cylindrica.

[0030] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0031] The above-mentioned weight parts of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae are washed, dried, and then added with water, the added amount of water being 8 times the total weight of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae, soaked for 30 minutes, then boiled with a large fire, and then boiled with a small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the total weight of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and the filtrate 2, concentrated to 1 g / mL of crude drug, prepared into a decoction, and then obtained.

[0032] Example 2, a traditional Chinese medicine composition for relieving gouty arthritis

[0033] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and weight parts: Herba Trichosanthis 22.5 parts, Herba Elsholtziae 11.25 parts, and Rhizoma Imperatae 11.25 parts.

[0034] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0035] The above-mentioned weight parts of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae are washed, dried, and then added with water, the added amount of water being 8 times the total weight of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae, soaked for 30 minutes, then boiled with a large fire, and then boiled with a small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the total weight of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and the filtrate 2, concentrated to 1 g / mL of crude drug, prepared into a decoction, and then obtained.

[0036] Example 3, a traditional Chinese medicine composition for relieving gouty arthritis

[0037] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and weight parts: Herba Trichosanthis 11.25 parts, Herba Elsholtziae 22.5 parts, and Rhizoma Imperatae 11.25 parts.

[0038] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0039] The above-mentioned weight parts of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae are washed, dried, and then added with water, the added amount of water being 8 times the total weight of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae, soaked for 30 minutes, then boiled with a large fire, and then boiled with a small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the total weight of the Herba Trichosanthis, Herba Elsholtziae and Rhizoma Imperatae, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and the filtrate 2, concentrated to 1 g / mL of crude drug, prepared into a decoction, and then obtained.

[0040] Example 4, a traditional Chinese medicine composition for relieving gouty arthritis

[0041] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and weight fractions: 11.25 parts of Itea orientalis, 11.25 parts of Mosla chinensis, and 22.5 parts of Imperata cylindrica.

[0042] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0043] The above Itea orientalis, Mosla chinensis, and Imperata cylindrica are washed, dried, and then added with water, the added amount of water being 8 times the total weight of Itea orientalis, Mosla chinensis, and Imperata cylindrica, soaked for 30 minutes, then boiled with large fire, and then boiled with small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the weight of Itea orientalis, Mosla chinensis, and Imperata cylindrica, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and filtrate 2, concentrated to 1 g / mL of crude drug amount, prepared into a decoction, and then obtained.

[0044] Comparative Example 1: A traditional Chinese medicine for relieving gouty arthritis

[0045] The traditional Chinese medicine for relieving gouty arthritis comprises the following raw materials and weight fractions: 45 parts of Itea orientalis.

[0046] The preparation method of the traditional Chinese medicine for relieving gouty arthritis is as follows:

[0047] The above Itea orientalis is washed, dried, and then added with water, the added amount of water being 8 times the weight of Itea orientalis, soaked for 30 minutes, then boiled with large fire, and then boiled with small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the weight of Itea orientalis, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and filtrate 2, concentrated to 1 g / mL of crude drug amount, prepared into a decoction, and then obtained.

[0048] Comparative Example 2: A traditional Chinese medicine for relieving gouty arthritis

[0049] The traditional Chinese medicine for relieving gouty arthritis comprises the following raw materials and weight fractions: 45 parts of Imperata cylindrica.

[0050] The preparation method of the traditional Chinese medicine for relieving gouty arthritis is as follows:

[0051] The above Imperata cylindrica is washed, dried, and then added with water, the added amount of water being 8 times the weight of Imperata cylindrica, soaked for 30 minutes, then boiled with large fire, and then boiled with small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the weight of Imperata cylindrica, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and filtrate 2, concentrated to 1 g / mL of crude drug amount, prepared into a decoction, and then obtained.

[0052] Comparative Example 3: A traditional Chinese medicine for relieving gouty arthritis

[0053] The traditional Chinese medicine for relieving gouty arthritis comprises the following raw materials and weight fractions: 45 parts of Mosla chinensis.

[0054] The preparation method of the traditional Chinese medicine for relieving gouty arthritis is as follows:

[0055] The above-mentioned Elsholtzia argyi is washed, dried, and then added with water, the added amount of water being 8 times the weight of the Elsholtzia argyi, soaked for 30 minutes, then boiled with a large fire, and then boiled with a small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the weight of the Elsholtzia argyi, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and the filtrate 2, concentrated to 1 g / mL of crude drug, prepared into a decoction, and then obtained.

[0056] Comparative Example 4, a traditional Chinese medicine composition for relieving gouty arthritis

[0057] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and their weight parts: 22.5 parts of Itea chinensis and 22.5 parts of Imperata cylindrica.

[0058] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0059] The above-mentioned Itea chinensis and Imperata cylindrica are washed, dried, and then added with water, the added amount of water being 8 times the total weight of the Itea chinensis and the Imperata cylindrica, soaked for 30 minutes, then boiled with a large fire, and then boiled with a small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the total weight of the Itea chinensis and the Imperata cylindrica, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and the filtrate 2, concentrated to 1 g / mL of crude drug, prepared into a decoction, and then obtained.

[0060] Comparative Example 5, a traditional Chinese medicine composition for relieving gouty arthritis

[0061] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and their weight parts: 22.5 parts of Itea chinensis and 22.5 parts of Elsholtzia argyi.

[0062] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0063] The above-mentioned Itea chinensis and Elsholtzia argyi are washed, dried, and then added with water, the added amount of water being 8 times the total weight of the Itea chinensis and the Elsholtzia argyi, soaked for 30 minutes, then boiled with a large fire, and then boiled with a small fire for 1 hour, filtered to obtain filtrate 1, added with water to the residue, the added amount of water being 8 times the total weight of the Itea chinensis and the Elsholtzia argyi, boiled, filtered to obtain filtrate 2, combined the filtrate 1 and the filtrate 2, concentrated to 1 g / mL of crude drug, prepared into a decoction, and then obtained.

[0064] Comparative Example 6, a traditional Chinese medicine composition for relieving gouty arthritis

[0065] The traditional Chinese medicine composition for relieving gouty arthritis comprises the following raw materials and their weight parts: 22.5 parts of Elsholtzia argyi and 22.5 parts of Imperata cylindrica.

[0066] The preparation method of the traditional Chinese medicine composition for relieving gouty arthritis is as follows:

[0067] The above-mentioned Elsholtzia and Imperata cylindra were washed and dried, and then water was added in an amount of 8 times the total weight of the Elsholtzia and Imperata cylindra. After soaking for 30 minutes, boiling was performed with a large fire, and then decoction was performed with a small fire for 1 hour. Filtrate 1 was obtained by filtration. Water was added to the residue in an amount of 8 times the total weight of the Elsholtzia and Imperata cylindra, and then decoction was performed, and filtrate 2 was obtained by filtration. Filtrate 1 and filtrate 2 were combined, concentrated to a crude drug amount of 1 g / mL, and prepared into a decoction to obtain the traditional Chinese medicine composition for relieving gouty arthritis.

[0068] 1. Experimental materials

[0069] 1.1 Experimental animals

[0070] Table 1 Animal information

[0071]

[0072]

[0073] 1.2 Drugs and reagents

[0074] The Imperata cylindra, Gynostemma pentaphyllum and Elsholtzia used in the experiment were identified by Professor Wang Honggang of Guangdong University of Chinese Medicine.

[0075] Table 2 Drug information

[0076]

[0077] Table 3 Reagent information

[0078] Name Batch No. Factory Normal saline H24031904 Guangdong Kelun Pharmaceutical Co., Ltd. Paraformaldehyde C11579047 Mcclin Biotech Co., Ltd. Isoflurane 2024101501 Shandong Ante Animal Husbandry Technology Co., Ltd. PBS buffer GA24080190624 Wuhan Saiver Biological Technology Co., Ltd. Sodium urate JS261820 Shanghai Yuan Ye Biological Technology Co., Ltd. Tween 80 20200601009 Tianjin Zhiyuan Chemical Reagent Co., Ltd. Anhydrous ethanol (analytical pure) 20220201310 Tianjin Zhiyuan Chemical Reagent Co., Ltd.

[0079] Table 4 Kit information

[0080]

[0081]

[0082] 1.3 Experimental instruments

[0083] Table 5 Experimental instruments

[0084]

[0085] 2. Experimental method

[0086] 2.1 Experimental grouping

[0087] The 36 SD rats were divided into 12 groups, namely a blank group, a model group, 4 example groups and 6 comparative example groups, 3 rats in each group.

[0088] 2.2 Modeling: After 1 h of administration on the 5th day, rats except the blank group were anesthetized with isoflurane, and the right ankle joint lateral rear of the rats was selected as the puncture point. 0.2 mL (25 mg / mL) of sodium urate suspension was injected into the right hind ankle joint cavity of the rats using a 1 mL disposable sterile injection needle. The injection standard was the swelling of the contralateral joint capsule. After injection, the surface skin turned red, swelled, and was hot, and the right foot was lame, which indicated that the modeling was successful.

[0089] 2.3 Administration: The groups of each example and the groups of the comparative examples were administered by gavage at a dose of 4.725 g / kg, and the blank group and the model group were administered with normal saline by gavage once a day for 7 consecutive days.

[0090] 2.4 Observation index:

[0091] 2.4.1 Detection of general signs of rats: From the 1st day of the experiment, the general state, body weight, fur, and excretion of rats were observed and recorded. The body weight of all rats was measured every day, and the data were recorded and saved for analysis of the effect of the drug on the body weight of the gouty arthritis rat model.

[0092] 2.4.2 Inflammation index and gait index: The inflammation index and gait index of the ankle joint of all rats were scored at 0 h, 6 h, 12 h, 24 h, 48 h, and 72 h after modeling. The scoring criteria are shown in Tables 6 and 7 (Note: If the grade is between two grades, the intermediate value is counted. For example, if the inflammation index grade is between 0 and 1, 1 point is counted).

[0093] Table 6 Inflammation index scoring criteria

[0094] Grade Inflammatory manifestations 0 level, 0 points Normal joints, no redness, swelling, fever 1 level, 2 points Redness, mild swelling of joint skin, but bone landmarks are still visible 2 level, 4 points Joint site is obviously red and swollen, and bone landmarks disappear 3 level, 6 points Redness and swelling of limbs other than joints

[0095] Table 7 Gait index scoring criteria

[0096]

[0097]

[0098] 2.5.3 Swelling degree of ankle joint: The diameters of the same region of the right ankle joint of all rats before modeling and at 0 h, 6 h, 12 h, 24 h, 48 h, and 72 h after modeling were measured by a vernier caliper, and the swelling degree of the ankle joint of the rats was recorded.

[0099] 2.5.4 Organ index: The liver and kidney of the rats were taken, washed with normal saline, and the water was absorbed with filter paper. The weight of the organs was accurately measured, and the organ index was calculated. Organ index (%) = organ weight (mg) / body weight of rat (g).

[0100] 2.5.5 Biochemical index: The serum samples of the rats were taken, and the determination of the contents of TNF-α, IL-1β, and IL6 was performed according to the operation of the Elisa kit.

[0101] 2.5.6 Statistical analysis: All experimental data were analyzed by SPSS 22.0 statistical software, and the results were expressed as mean ± standard deviation (X ± S). The data between groups were analyzed by one-way ANOVA, and P<0.05 was considered statistically significant. If the data met the homogeneity of variance, LSD-t test was used to analyze the differences between groups; if the data did not meet the homogeneity of variance, Tukey's method was used for comparison between groups.

[0102] 3. Experimental results

[0103] 3.1 General state of rats: The effect of drugs on the body weight of rats is shown in Table 8. During the experiment, the body weight of rats in each group increased steadily, the rats had normal diet and water intake, the fur was lustrous, the mental state was good, the feces shape was normal, there was no loose stool, and there was no irritation to the gastrointestinal tract. As can be seen from Table 8, the drugs had no significant effect on the growth of rats.

[0104] Table 8 Effect of drugs on the body weight of rats n=3

[0105]

[0106]

[0107]

[0108] 3.2 Organ index: The effect of drugs on liver and kidney index is shown in Table 9. During the drug administration period, there was no significant difference in liver and kidney index of rats in each group, indicating that the drug treatment process had no obvious liver and kidney toxicity, and the safety was high.

[0109] Table 9 Effect of drugs on liver and kidney index n=3

[0110]

[0111]

[0112] 3.3 Joint swelling degree: The change diagram of ankle joint of gouty arthritis rat model is shown in Figure 1 , specifically, Figure 1 is the comparison of left and right feet of the same rat, the right ankle joint is modeled, and the left ankle joint is not modeled; among them Figure 1 is the front view, Figure 1 is the plan view, and it can be observed that the right ankle joint is obviously swollen. The joint swelling degree of rats in each modeling group increased significantly, indicating that the modeling of gouty arthritis was successful. The comparison diagram of ankle joint of rats in each group 72h after modeling is shown in Figure 2 , and the effect of drugs on the swelling degree of ankle joint of rats is shown in Table 10. From Figure 2As can be seen from Table 10, under the intervention of the rest of the groups of drugs, the joint swelling degree of the rats is reduced to different degrees, and the reduction of the four example groups is more obvious. The results show that, compared with the blank group, the ankle joint swelling index of the model group at 0h, 6h, 12h, 24h, 48h and 72h after modeling is significantly increased (P<0.001, P<0.01), and except for example 1, the ankle joint swelling index of the rest of the groups is the highest at 12h after modeling, indicating that the ankle joint swelling degree reaches the peak; compared with the model group, the joint swelling degree of example 1 is significantly reduced (P<0.01) at 12h after modeling; the ankle joint swelling degree of each example is significantly reduced (P<0.01, P<0.05) at 24h after modeling.

[0113] Table 10 Influence of drugs on ankle joint swelling degree of rats n=3

[0114]

[0115]

[0116]

[0117] Note: compared with the blank group, ### P<0.001, ## P<0.01, # P<0.05; compared with the model group, *** P<0.001, ** P<0.01, * P<0.05.

[0118] 3.4 Inflammation index and gait index:

[0119] The influence of drugs on the inflammation index of rats is shown in Table 11, and the results show that, compared with the blank group, the inflammation index of the model group at 0h, 6h, 12h, 24h, 48h and 72h after modeling is significantly increased (P<0.001, P<0.05); compared with the model group, the inflammation index of example 3 is significantly reduced (P<0.001) at 24h after modeling, the inflammation index of example 1 is significantly reduced (P<0.01), the inflammation index of example 2 and example 4 is significantly reduced (P<0.05), and the inflammation index of example 1 is significantly reduced (P<0.05) at 48h after modeling, indicating that the present application can relieve the inflammation state of rats.

[0120] The influence of the drugs on the gait index of the rats is shown in Table 12. After the rats in each group were administered the drugs by gavage, the gait index of the rats in each example was lower than that in the model group, indicating that the application can alleviate the action function state of the rats. The results show that, compared with the blank group, the gait index of the model group at each time point after modeling was significantly increased (P<0.001, P<0.01); compared with the model group, the gait index of Example 1 was significantly reduced at 0h and 72h after modeling (P<0.01, P<0.01), the gait index of Example 2 and Example 3 was significantly reduced at 24h and 72h after modeling (P<0.05, P<0.05), and the gait index of Example 4 was significantly reduced at 24h after modeling (P<0.05).

[0121] Table 11 Influence of drugs on inflammation index of rats n = 3

[0122]

[0123]

[0124]

[0125] Note: compared with the blank group, ### P<0.001, ## P<0.01, # P<0.05; compared with the model group, *** P<0.001, ** P<0.01, * P<0.05.

[0126] Table 12 Influence of drugs on gait index of rats n = 3

[0127]

[0128]

[0129] Note: compared with the blank group, ### P<0.001, ## P<0.01, # P<0.05; compared with the model group, *** P<0.001, ** P<0.01, * P<0.05.

[0130] 3.5 Influence on inflammatory factors in serum

[0131] The influence of the drugs on the content of inflammatory factors of rats is shown in Table 13. The results show that, compared with the blank group, the content of IL-1β, IL-6 and TNF-α in the serum of the model group of rats is significantly increased (P<0.01; P<0.05; P<0.001). Compared with the model group, the content of IL-1β in the example 2 group and the example 4 group is significantly reduced (P<0.01, P<0.05); the content of IL-6 in the comparative example 3, the comparative example 6, the example 2 and the example 3 is significantly reduced (P<0.05), wherein the content of IL-6 in the example 3 is the lowest; the content of TNF-α in the comparative example 2, the comparative example 5 and the example 4 is significantly reduced (P<0.05), and the content of TNF-α in the example 3 is significantly reduced (P<0.01); the content of TNF-α in the example 1 is extremely significantly reduced (P<0.001) (362.93±21.20 ng / L).

[0132] The results show that, after the intervention of the drugs of each group, the content of IL-1β, IL-6 and TNF-α of the rats of each comparative example and example is reduced to different degrees, but the reduction of the examples is more obvious, which indicates that the combination of the Folium Pteridis multifidae, Herba Elsholtzae and Rhizoma Imperatae can more effectively reduce the level of inflammatory related cytokines and relieve the inflammatory state of rats.

[0133] Table 13 Influence of drugs on the content of inflammatory factors of rats n=3

[0134] Grouping IL-1β (ng / L) IL-6 (pg / ml) TNF-α (ng / L) Blank group 39.68 ± 0.57 ** ]] 114.75 ± 0.64 * ]] 364.52 ± 3.54 *** ]] Model group 49.99 ± 0.73 ## ]] 156.55 ± 2.02 # ]] 417.54 ± 4.83 ### ]] Comparative example 1 46.80±1.51 140.95±3.10 399.58±3.74 Comparative example 2 45.96±0.18 137.57±3.84 387.18 ± 10.67 * <!-- 17 -->]] Comparative example 3 46.62±1.36 132.46 ± 1.40 * ]] 411.76±8.71 Comparative example 4 45.77±2.28 139.42±3.18 395.66±18.33 Comparative example 5 42.70±3.09 132.86±3.65 386.34 ± 17.19 * ]] Comparative example 6 42.44±1.65 135.27 ± 1.44 * ]] 391.47±21.91 Example 1 42.35±1.17 134.07±4.19 362.93 ± 21.20 *** ]] Example 2 41.71 ± 0.65 ** ]] 137.18 ± 2.06 * ]] 393.26±10.61 Example 3 43.67±1.82 124.36 ± 0.67 * ]] 372.74 ± 25.13 ** ]] Example 4 42.76 ± 0.85 * ]] 130.78±8.16 389.60 ± 21.08 * ]]

[0135] Note: compared with the blank group, ### P<0.001, ## P<0.01, S P<0.05; compared with the model group, *** P<0.001, ** P<0.01, * P<0.05.

[0136] 3.6 Conclusion

[0137] According to the above experimental results, the technical scheme provided by the present application has a significant effect in relieving inflammation and improving related symptoms.

[0138] Compared with the blank group, the IL-1β, IL-6 and TNF-α levels of the model group were significantly increased (P<0.01, P<0.05, P<0.001), indicating that the modeling was successful, and the model rats had obvious inflammatory response. After the intervention of the various comparative examples and the example drugs, the IL-1β, IL-6 and TNF-α levels of the rats were reduced to different degrees, and the reduction of the example group was more obvious. The IL-1β level of Example 2 and Example 4 was significantly reduced; the IL-6 content of Example 2 and Example 3 was significantly reduced; the TNF-α level of Example 1, Example 3 and Example 4 was significantly reduced, and the effect of Example 1 on reducing the TNF-α level was extremely significant (P<0.001). This indicates that the technical scheme of the application can effectively reduce the level of inflammatory cytokines and relieve the inflammatory state of the rats. In combination with the experimental results of the inflammation index and the gait index, the example shows good effects in relieving the inflammatory state and the action function state of the rats, the inflammation index of the example is significantly reduced at a specific time point compared with the model group, and the gait index is also reduced to different degrees, which further verifies the effectiveness of the technical scheme of the application.

[0139] In summary, the technical scheme of the application can effectively relieve the inflammatory response, improve the joint dysfunction caused by inflammation, has important application value and promotion prospect, and provides a new idea and method for the treatment of related diseases.

Claims

1. A traditional Chinese medicine composition for relieving gouty arthritis, characterized in that, The ingredients and their weight proportions are as follows: 10-45 parts of gourd tea, 10-45 parts of fragrant sesame, and 10-45 parts of white peony root.

2. The traditional Chinese medicine composition for relieving gouty arthritis according to claim 1, characterized in that, The ingredients include the following ingredients and their weight proportions: 11.25-22.5 parts of gourd tea, 11.25-22.5 parts of sesame, and 11.25-22.5 parts of Imperata cylindrica root.

3. The traditional Chinese medicine composition for relieving gouty arthritis according to claim 2, characterized in that, The ingredients include the following ingredients and their weight proportions: 11.25-22.5 parts of gourd tea, 11.25-15 parts of fragrant sesame, and 11.25-22.5 parts of Imperata cylindrica root.

4. The traditional Chinese medicine composition for relieving gouty arthritis according to claim 3, characterized in that, The ingredients and their weight proportions are as follows: 22.5 parts of gourd tea, 15 parts of fragrant sesame, and 15 parts of white peony root.

5. The method for preparing the traditional Chinese medicine composition for relieving gouty arthritis according to any one of claims 1 to 4, characterized in that, Includes the following steps: Wash the gourd tea, sesame, and imperata root, dry them, add water (6-10 times the total weight of the gourd tea, sesame, and imperata root), soak for 20-40 minutes, then decoct and filter to obtain filtrate 1. Add water to the dregs (6-10 times the total weight of the gourd tea, sesame, and imperata root), decoct and filter to obtain filtrate 2. Combine filtrate 1 and filtrate 2, concentrate, and you have the final product.

6. The method for preparing the traditional Chinese medicine composition for relieving gouty arthritis according to claim 5, characterized in that, Add water in an amount eight times the total weight of the gourd tea, sesame, and imperata root, soak for 30 minutes, and then decoct.

7. The method for preparing the traditional Chinese medicine composition for relieving gouty arthritis according to claim 5, characterized in that, The specific steps for simmering are as follows: bring to a boil over high heat, then simmer over low heat for 0.5 to 1.5 hours.

8. The method for preparing the traditional Chinese medicine composition for relieving gouty arthritis according to claim 7, characterized in that, Simmer over low heat for 1 hour.

9. The use of the traditional Chinese medicine composition for relieving gouty arthritis according to any one of claims 1 to 4, or the traditional Chinese medicine composition for relieving gouty arthritis according to any one of claims 5 to 8, in the preparation of a medicament for relieving gouty arthritis.

10. A drug for relieving gouty arthritis, characterized in that, It includes the traditional Chinese medicine composition for relieving gouty arthritis as described in any one of claims 1 to 4 and pharmaceutically acceptable excipients.

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