Preparation-and-use type plasma activated water wound surface cleaning treatment device and preparation-and-use type plasma activated water wound surface cleaning treatment method

Abstract

The invention relates to the field of plasma technology and medical equipment. The invention provides a ready-to-use plasma activated water wound cleaning treatment device and method.The ready-to-use plasma activated water wound cleaning treatment device comprises a box body, a power module is arranged in the box body, an electrode assembly is integrated in the power module and comprises a ground electrode and a high-voltage electrode, the ground electrode is of a woven metal mesh bandage structure, and the high-voltage electrode is integrated in the power module; the high-voltage electrode is wrapped by a quartz tube with breathable micropores in the bottom, the quartz tube is connected with an air pump in the box body through an air pipe, and the high-voltage electrode is connected with a power module through a high-voltage wire. According to the invention, rapid, stable and batch preparation of plasma activated water required by a wound infection area can be realized, so that the treatment efficiency is improved, and the use process is simplified. According to the device, activated water rich in active substances is efficiently generated through plasma discharge, and the device can be directly used for local sterilization, anti-inflammation and healing promotion treatment of wound surfaces, so that the clinical requirement for instant preparation and instant use is met.

Description

Technical Field

[0001] This invention relates to the fields of plasma technology and medical equipment, and in particular to a device and method for on-the-go plasma-activated water wound cleaning treatment. Background Technology

[0002] Plasma-activated water (PAW) is being applied in wound treatment. This innovative therapy is based on an active liquid system generated by low-temperature plasma excitation. It fully utilizes its rich content of various active substances (such as H₂O₂, NO₂⁻, and ·OH) to exhibit excellent antibacterial, anti-inflammatory, and tissue-regulating capabilities, potentially overcoming many limitations of traditional wound treatment methods. PAW possesses broad-spectrum bactericidal capabilities, effectively eliminating bacteria, fungi, and viruses in wounds, particularly showing good efficacy against antibiotic-resistant strains. Compared to traditional antibiotic dressings or chemical disinfectants, PAW, due to its low temperature and high biocompatibility, significantly reduces the risk of irritation and thermal damage to wound tissues during use, making it suitable for various acute and chronic wounds, including burns, diabetic foot ulcers, and complex surgical incisions. Furthermore, the active substances in PAW can penetrate deep into the wound microenvironment, overcoming biofilm barriers that traditional drugs struggle to penetrate, which is of great significance for treating chronic, refractory, and recurrent wound infections. As a non-invasive, low-irritant, and highly safe treatment method, plasma-activated water (PAW) is expected to improve patient compliance, reduce treatment cycles, and enhance overall prognosis. With the continuous development of low-temperature plasma and its liquid media application technologies and the deepening of clinical validation, PAW is poised to become a safe, efficient, and personalized new treatment option in wound management, providing a superior solution for infection control and tissue repair.

[0003] Existing low-temperature plasma activated water preparation devices still have certain limitations in terms of structural design and functional implementation. For example, although a two-stage ionization structure with an ionization chamber and a water chamber connected in series is adopted, the ionization efficiency is heavily dependent on a fixed structural layout, and the electric field distribution is difficult to adjust flexibly, resulting in poor adaptability to changes in operating parameters. Furthermore, these devices are large and cumbersome to use, relying mainly on initially set gas-liquid paths and component arrangements, lacking real-time adjustment capabilities, and hindering precise control of output parameters according to different clinical conditions or usage requirements. In addition, due to the relatively simple gas-liquid contact method, the reaction interface between gas and liquid is limited, affecting the depth and uniformity of plasma interaction. The overall system integration is low, with numerous pipeline connections and a complex structure, increasing size and hindering miniaturization and modular integration. Some devices do not effectively suppress exhaust gas generation through gas path design, still requiring a back-end exhaust gas treatment module to simply guide or purify emissions, failing to achieve zero exhaust gasification or minimized exhaust gas control at the source, which is completely insufficient for applications with high requirements for clinical environmental friendliness and safety. Summary of the Invention

[0004] The technical problem to be solved: How can the plasma activated water (PAW) wound cleaning and treatment device achieve rapid, stable, and batch preparation of plasma activated water required for infected wound areas, thereby improving treatment efficiency and simplifying the usage process? How can it solve the problems of large equipment size, low integration, unstable active substances, and inability to achieve on-the-spot preparation in existing technologies?

[0005] Technical solution: To address the above problems, this invention provides a ready-to-use plasma-activated water wound cleaning device, comprising a housing, a power module housed inside the housing, and an electrode assembly integrated within the power module. The electrode assembly includes a ground electrode and a high-voltage electrode. The ground electrode is a woven metal mesh bandage structure. The high-voltage electrode is wrapped by a quartz tube with breathable micropores at the bottom. The quartz tube is connected to an air pump inside the housing via an air pipe. The high-voltage electrode is connected to the power module via a high-voltage wire.

[0006] The power module includes a self-dielectric DBD micropore bubble discharge electrode, an air pump unit, a DC bus control unit controlled by an STM32 CNC unit, a human-machine interface control unit, and a PWM generator unit. The DC bus control unit controls the AC-DC programmable bus power unit to output DC power to the Marx main power circuit unit through electrical connection lines. The Marx main power circuit unit supplies the high voltage required by the high-voltage electrode through a high-voltage line. Simultaneously, the air pump unit supplies air to the quartz tube. A magnetically isolated drive unit and a PWM generator unit are also provided, connected to the Marx main power module, generating control signals to the primary winding of the magnetic ring of the Marx main power module. The magnetic isolation drive unit uses a single insulated wire to directly pass through the nano-amorphous magnetic ring to form the primary side circuit, and the secondary side uses a 3-turn winding. The magnetic isolation drive unit includes two high-speed switching transistors. The primary side uses a half-bridge circuit structure as the excitation source, and its alternating conduction is controlled by the front-stage PWM signal.

[0007] The high-voltage electrode is a metal needle, and the metal needle has a high-voltage electrode shell on the outside of the quartz tube. One end of the metal needle is connected to a high-voltage wire.

[0008] The quartz tube is fitted with a protective sleeve.

[0009] A hollow flexible tube is provided, and the air pipe and high-voltage wire are combined and installed inside the hollow flexible tube.

[0010] The box body is equipped with a mechanical button control panel, and the mechanical button control panel is equipped with mechanical knobs.

[0011] It also includes an HMI (Human-Machine Interface) control panel, which integrates a touch screen display. Users can directly select the required preparation parameters on the screen, including discharge time, discharge voltage, water volume and pulse parameters, and the current operating status and remaining time information are displayed on the screen simultaneously.

[0012] This invention also provides a method for on-demand plasma-activated water wound cleaning, using the aforementioned on-demand plasma-activated water wound cleaning device, comprising the following steps: Step S01: Remove the ground electrode, electrode structure air tube, high-voltage wire, high-voltage electrode, high-voltage electrode shell, quartz tube and protective sleeve, and pull out the protective sleeve; Step S02: Connect the mains power socket; Step S03: The ground electrode is a woven metal mesh bandage structure that wraps around the treatment solution bottle and serves as the dielectric layer in the DBD mode; Step S04: Turn on the air pump to continuously introduce air into the quartz glass tube, insert the quartz tube containing the high-voltage electrode into the solution to generate microbubbles in the solution; select the corresponding solution specification on the HMI human-machine interface, then input the voltage parameters, and then start the device on the screen to begin the preparation and activation; Step S05: The preparation will stop automatically after the preparation time is over. The activated water preparation is complete. Take the prepared activated water and wipe it on the wound.

[0013] In step S04, the voltage and current parameters of the input DC power are observed through the mechanical control panel. If the screen is interrupted and cannot be operated, the voltage is adjusted and the power is cut off in an emergency by using the mechanical buttons on the mechanical control panel.

[0014] Beneficial effects: This device features a highly integrated design, a compact and lightweight structure, and is easy to carry and use. It achieves efficient generation of plasma-activated water rich in active substances at room temperature and pressure, making it particularly suitable for various applications such as bedside treatment, outdoor emergency wound care, and daily home care. This portable design not only significantly improves the device's applicability but also dramatically enhances the efficiency of wound infection control and treatment effectiveness, providing a completely new solution for medical care. Furthermore, it enables the rapid, stable, and batch preparation of plasma-activated water required for infected wound areas, thereby improving treatment efficiency and simplifying the usage process. The device efficiently generates activated water rich in active substances through plasma discharge, which can be directly used for local wound sterilization, anti-inflammation, and wound healing promotion, meeting the clinical need for immediate use.

[0015] This invention employs innovative magnetic isolation drive synchronization technology. Through optimized design of the primary-side single-turn series core structure and the secondary-side gate drive circuit, it achieves three core advantages: 1) It breaks through the synchronization problem of high-voltage signal transmission, ensuring stable system operation; 2) It significantly optimizes the edge characteristics of nanosecond-level pulses; 3) Compared with traditional fiber optic solutions, it requires fewer components, reduces size, and combines high reliability with extreme compactness. This technology enables the device to efficiently generate plasma-activated water at room temperature and pressure, providing a breakthrough solution for portable medical devices.

[0016] The replaceable self-dielectric DBD microporous bubble discharge electrode structure allows for the replacement of different ground electrodes according to different usage requirements.

[0017] The electrodes are housed inside the power supply, and the integrated design makes it easier to carry and use, while also effectively preventing damage. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the entire device.

[0019] Figure 2It is a block diagram of the overall constituent units.

[0020] Figure 3 This is a schematic diagram of a magnetically isolated drive.

[0021] Figure 4 It is an operation flowchart.

[0022] Figure 5 These are the discharge states and electrical waveforms of PAW fabrication, where 5(a) is the nanosecond pulse drive and 5(b) is the nanosecond pulse discharge waveform.

[0023] Figure 6 This is a schematic diagram showing the temperature change over time when different electrodes are used to prepare PAW.

[0024] Figure 7 This is a schematic diagram illustrating the inactivation effect of PAW on Pseudomonas aeruginosa.

[0025] Figure 8 The diagrams show the bactericidal rates of PAW against two types of Vibrio at different storage and treatment times. 8(a) shows the bactericidal rate of PAW against Vibrio vulnificus at different storage and treatment times, and 8(b) shows the bactericidal rate of PAW against Vibrio parahaemolyticus at different storage and treatment times.

[0026] Explanation of reference numerals in the attached diagram: 1. Ground electrode; 2. Mechanical button control panel; 3. Mechanical knob; 4. HMI human-machine interface control panel; 5. Air tube and high-voltage wire assembly; 6. High-voltage electrode housing; 7. Quartz tube; 8. Protective sleeve; 9. Mains power socket. Detailed Implementation

[0027] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0028] This invention provides a ready-to-use plasma activated water (PAW) wound cleaning and treatment device, which is mainly used for efficient spectral sterilization of wounds, ready-to-use, and portable. It solves the problems of large device size, low integration, unstable active substances, and inability to achieve ready-to-use in the prior art.

[0029] The specific structure of a ready-to-use plasma activated water (PAW) wound cleaning and treatment device is as follows: Figure 1 As shown, the device includes a housing, with the power module located inside the housing and the electrode assembly integrated inside the power module. This achieves miniaturization and modularity of the device, allowing the electrodes to be completely stored inside the power housing when not in operation, effectively avoiding the risks of bumps, contamination, or accidental electric shock caused by exposed electrodes.

[0030] The electrode assembly includes a ground electrode 1 and a high-voltage electrode. The ground electrode 1 is a woven metal mesh bandage structure used to wrap the body of the treatment solution bottle. The solution bottle wall is made of high-precision polyethylene (HDPE) material, which serves as the medium layer in the DBD mode to form a "self-medium".

[0031] The high-voltage electrode can be replaced due to corrosion caused by repeated use, and the ground electrode can be selected in different sizes for different types of solution bottles to ensure uniform electric field distribution.

[0032] The high-voltage electrode is encased in a quartz tube 7 with permeable micropores at the bottom. The quartz tube 7 is connected to an air pump inside the chamber via an air pipe, and the high-voltage electrode is connected to a power module via a high-voltage wire. The air pump continuously supplies air into the quartz tube 7, inserting the high-voltage electrode into the solution. Due to the low resistivity of the solution, it can be considered an equipotential body, and through the action of the self-medium, a high voltage is generated between the high-voltage electrode and the solution. Small bubbles blown out with the air, due to their low air content and low breakdown voltage, will first generate plasma discharge within the bubbles, producing active particles. The bubbles then enter the preparation solution bottle through the permeable micropores at the bottom of the quartz tube 9. Because of the presence of active particles inside, the bubbles will burst while still in the solution due to particle movement, and the active particles will enter the solution and form PAW (Polymerized Active Wheat).

[0033] When not in use, the plasma activated water (PAW) wound cleaning and treatment device has the ground electrode 1, electrode structure air pipe, high-voltage wire, high-voltage electrode, high-voltage electrode shell 6, and quartz tube 7 stored inside the box. When in use, they are taken out.

[0034] In one embodiment, 8. further includes an HMI (Human-Machine Interface) control panel 4, which integrates a touch screen display. Users can directly select the required preparation parameters on the screen, including discharge time, discharge voltage, water volume, and pulse parameters, and the current operating status and remaining time information are simultaneously displayed on the screen. This plasma-activated water preparation method, which uses a screen for human-machine interaction, aims to improve the ease of operation and intelligent operation of the device. Through the integrated touch screen display, the user selects the required solution specifications, sets the preparation parameters, and controls the power-on start / stop.

[0035] The preparation parameters include key process conditions such as discharge time, discharge voltage, water volume, and pulse parameters, and the current operating status and remaining time are displayed simultaneously on the screen. Users can directly select the required preparation parameters on the screen, simplifying the traditional manual adjustment steps, lowering the user threshold, and improving the accuracy and repeatability of the operation. In one embodiment, a mechanical button operating panel 2 is provided outside the housing, and a mechanical knob 3 is provided on the mechanical button operating panel. The operating panel of this invention is composed of mechanical buttons and a screen interaction system, combining the intuitive reliability of traditional physical buttons with the intelligent convenience of a graphical user interface.

[0036] This invention involves wrapping a ground electrode around a saline bottle. After connecting to mains power, a high-voltage electrode is inserted into the saline bottle. Bubbles are blown out through the micropores at the bottom of the electrode's quartz tube. Voltage parameters are input through the HMI (Human-Machine Interface) screen, and the start button is pressed to begin preparing activated water. The preparation automatically stops after the preparation time is over. Once the activated water preparation is complete, a medical cotton ball is used with tweezers to apply the prepared activated water to the wound.

[0037] The overall component block diagram is as follows Figure 2 As shown, it is divided into a magnetically isolated drive unit, an STM32 numerical control unit, a Marx main power circuit unit, a bus power unit, a self-dielectric DBD micropore bubble discharge electrode, and an air pump unit. The STM32 numerical control unit controls three modules—the DC bus control unit, the human-machine interface control unit, and the PWM generation unit. The DC bus control unit controls the AC-DC programmable bus power unit to output DC power to the Marx main power circuit unit via electrical connections. The Marx main power circuit unit then supplies the high voltage required for discharge to the self-dielectric DBD micropore bubble discharge electrode via electrical connections. Simultaneously, the air pump unit supplies air to the self-dielectric DBD micropore bubble discharge electrode.

[0038] The Marx main power module: The gate circuit on the secondary side of the magnetic ring is controlled by the input signal of the half-bridge on the primary side of the magnetic ring, which converts the input of the programmable bus power supply into a high-voltage pulse output.

[0039] The bus power supply outputs stable DC power to the Marx main power module.

[0040] The human-machine interface control unit (HMI) communicates with the programmable bus power supply via 485.

[0041] The air pump unit supplies air to the high-voltage electrode quartz tube, causing it to generate microbubbles in the solution.

[0042] HMI Touchscreen: On the pre-designed screen, users can select the required solution specifications, set voltage parameters, and control power-on / off operation. The electrode structure comprises a high-voltage electrode and a ground electrode. The high-voltage electrode has an air tube connected to the air pump and a high-voltage output line connected to the power mainboard at the top, and a microporous quartz tube at the bottom. The ground electrode is available in three sizes to accommodate various solution specifications. The preparation capacity can be adjusted according to actual clinical needs. The device supports rapid activation of multiple liquid volumes, covering various application scenarios from small-volume individual wound treatment to medium-volume bedside care and even batch fluid supply. This significantly improves the system's versatility and adaptability, meeting the clinical requirements for personalized and refined treatment. In one embodiment, to achieve portability, integration, and ensure the stability of nanosecond pulse discharge, this invention abandons traditional fiber isolation or general magnetic isolation modules and designs a highly optimized magnetic isolation drive unit. The PWM generation unit and the magnetic isolation drive unit generate control signals on the ARM board and half-bridge circuit and send them to the primary side of the magnetic ring of the Marx main power module.

[0043] The structural principle of the magnetically isolated drive unit is as follows: Figure 3 As shown, this invention uses a single insulated wire to directly pass through a nanocrystalline amorphous magnetic ring to form the primary side circuit. This design minimizes the inherent leakage inductance and distributed capacitance of traditional multi-turn windings, laying a solid foundation for obtaining pulse waveforms with extremely fast rise / fall times (nanosecond level) from a physical structure perspective.

[0044] The secondary side employs a minimal 3-turn winding. This number of turns is precisely calculated, achieving the optimal balance between sufficient drive voltage, good magnetic coupling, and minimizing parasitic parameters. It ensures efficient energy and signal transmission while avoiding waveform ringing and edge deterioration caused by excessive turns.

[0045] A nanocrystalline amorphous magnetic ring with excellent high-frequency characteristics was selected as the magnetic core. This material has extremely high permeability, extremely low coercivity and high-frequency loss, which can efficiently couple the high-frequency components contained in nanosecond-level pulses, ensuring that the pulse waveform has low distortion and high fidelity during transmission. At the same time, its high saturation magnetic induction intensity avoids the risk of magnetic core saturation during pulse transmission, directly realizing the extreme miniaturization of the drive module.

[0046] The primary side of the magnetically isolated drive unit employs a half-bridge circuit structure as the excitation source. This structure consists of two high-speed switching transistors, which are alternately turned on by a preceding PWM signal. This generates a high-quality square wave with extremely steep edges and dead-time control to drive the primary side. The powerful current output capability provided by the half-bridge drive effectively overcomes the low inductance characteristics that may exist in a single-turn winding on the primary side of the transformer, rapidly establishing and removing the magnetic field, thereby greatly optimizing the rise speed of the pulse leading edge and the fall speed of the trailing edge. This design effectively prevents magnetic saturation of the magnetic ring, and the magnetic core can achieve automatic and perfect magnetic reset in each working cycle. This magnetic ring provides a stable, high-voltage, nanosecond pulse output for the series superposition of multi-stage pulse power circuits.

[0047] This invention proposes a magnetically isolated drive synchronization scheme, which effectively solves the signal transmission synchronization problem by using a single-turn series structure on the primary side in conjunction with a gate drive circuit on the secondary side. Compared with traditional fiber optic isolated drive schemes (high cost, complex structure, and large size), this transformer magnetically isolated drive scheme has advantages such as compact structure and fewer components. This design not only reduces the overall size but also optimizes the edge parameters of nanosecond pulses, achieving extreme pulse edge performance, thereby solving the discharge stability problem. Even in air, this device can still achieve efficient discharge plasma excitation.

[0048] This invention employs innovative magnetic isolation drive synchronization technology. Through optimized design of the primary-side single-turn series core structure and the secondary-side gate drive circuit, it achieves three core advantages: 1) It breaks through the synchronization problem of high-voltage signal transmission, ensuring stable system operation; 2) It significantly optimizes the edge characteristics of nanosecond-level pulses; 3) Compared with traditional fiber optic solutions, it requires fewer components, reduces size, and combines high reliability with extreme compactness. This technology enables the device to efficiently generate plasma-activated water at room temperature and pressure, providing a solution for portable medical devices.

[0049] In one embodiment, the high-voltage electrode of the present invention is a metal needle, and the metal needle has a high-voltage electrode housing 6 on the outer part of the quartz tube 7, and one end of the metal needle is connected to a high-voltage wire.

[0050] In one embodiment, a protective sleeve 8 is provided outside the quartz tube 7 to protect the high-voltage electrode. The protective sleeve 8 is sleeved on the head of the quartz tube 7, which can improve the mechanical safety of the glass tube dielectric barrier discharge (DBD) electrode.

[0051] In one embodiment, in order to better house the high-voltage electrode in the housing, a hollow flexible tube is provided, and the air pipe and high-voltage wire are placed inside the hollow flexible tube, forming an air pipe and high-voltage wire assembly 5.

[0052] This invention also provides a method for on-demand plasma-activated water wound cleaning, using the aforementioned on-demand plasma-activated water wound cleaning device, such as... Figure 4 As shown, it includes the following steps: Step S01: Remove the ground electrode 1, electrode structure air tube, high voltage wire, high voltage electrode, high voltage electrode shell 6, quartz tube 7 and protective sleeve 8, and pull out the protective sleeve 8. Step S02: Connect the mains power socket 9 to the power supply; Step S03: Ground electrode 1 is a woven metal mesh bandage structure that wraps around the treatment solution bottle and serves as the dielectric layer in the DBD mode; Step S04: Turn on the air pump to continuously introduce air into the quartz glass tube, insert the quartz tube 7 wrapped with the high voltage electrode into the solution, and generate microbubbles in the solution; select the corresponding solution specification on the HMI human-machine interface console 44, then input the voltage parameters, and then start the device on the screen to begin the preparation and activation. Step S05: The preparation will stop automatically after the preparation time is over. The activated water preparation is complete. Take the prepared activated water and wipe it on the wound.

[0053] In one implementation, during the preparation of activated water, the voltage and current parameters of the input DC power can be observed through the mechanical operating table 2. If the screen is interfered with and becomes unusable, the voltage can be adjusted and the power can be cut off in an emergency through the mechanical button 3 on the mechanical operating table 2.

[0054] The feasibility of plasma-activated water discharge is verified by the following experiment.

[0055] 1) Experimental verification of the stability of plasma-activated water discharge.

[0056] To verify the discharge stability of the plasma generated by the pulsed power-driven discharge method of this invention for preparing PAW (Pollution-Activated Wheatstone), the nanosecond pulse of this invention was used at 7 kV and 1 kHz, and 100 mL of physiological saline was selected to prepare PAW. The experiment showed that, as... Figure 5 As shown, the nanosecond pulse power supply of the present invention discharges uniformly and stably, as... Figure 5 As shown in (a). It can be seen from the discharge waveform that, as Figure 5 As shown in (b), the nanosecond pulse power supply of this work can obtain a large discharge current at a lower voltage amplitude, and the discharge current can characterize the discharge intensity, thus making PAW preparation more stable and efficient.

[0057] 2) Experimental verification of the repeatability of plasma-activated water preparation.

[0058] To verify the excellent repeatability of the on-demand, high-efficiency PAW wound cleaning and treatment device proposed in this invention, which ensures consistent active particle concentration in PAW under the same preparation parameters and time, 100 mL of physiological saline was used to prepare PAW. Using this integrated device with appropriate parameters: nanosecond pulse (7 kV, 1 kHz), PAW was prepared three times, each time for 10 min. Subsequently, a UV-Vis spectrophotometer (UV1800) was used to test the concentration of active substances in the activated water for each preparation. The different active particle concentrations measured in the three experiments are shown in Table 1.

[0059] surface The content of typical long-lived active RONS substances in PAW repeated preparation The results of three experiments show that the active particle components in the PAW prepared by the on-the-go high-efficiency PAW wound cleaning and treatment device proposed in this work have good repeatability.

[0060] 3) Experimental verification of discharge temperature of plasma-activated water solutions of different specifications.

[0061] Further verification of the proposed on-demand, high-efficiency PAW wound cleaning and treatment device demonstrates that PAW preparation can be maintained at a low temperature close to room temperature, thereby maximizing the lifespan of active particles and preventing their decomposition. The experiment compared the overall solution temperature during preparation of the self-dielectric DBD microporous gas-liquid discharge electrode developed in this work with that of a traditional arc discharge electrode. A thermal imager was used to record the overall temperature changes over time for both electrodes at preparation sizes of 100mL, 250mL, and 500mL, recording real-time temperatures at 0 min, 2 min, 4 min, 6 min, 8 min, and 10 min. Figure 6 As shown.

[0062] Figure 6 In the figure, A represents a traditional arc discharge electrode, and B represents a self-dielectric DBD microporous gas-liquid discharge electrode. As can be seen from the figure, regardless of the specifications of physiological saline used to prepare PAW (painful wound healing), the solution temperature at electrode A is significantly higher than that at electrode B. Furthermore, the smaller the amount of solution prepared, the greater the temperature difference. PAW prepared using the self-dielectric DBD microporous gas-liquid discharge electrode reaches thermal equilibrium within 5 minutes, at a temperature close to room temperature. Therefore, we can conclude that the experimental results show that, compared to traditional arc discharge electrodes, the self-dielectric DBD microporous gas-liquid discharge electrode developed in this high-efficiency, on-the-go PAW wound cleaning and treatment device effectively reduces the overall temperature of the PAW preparation solution.

[0063] 4) Experimental verification of the broad-spectrum bactericidal efficacy of plasma-activated water.

[0064] To verify the inhibitory effect of the prepared PAW on wound infection bacteria, the PAW prepared by this device was used to treat wound pathogens such as Pseudomonas aeruginosa, Vibrio vulnificus, and Vibrio parahaemolyticus to verify its inactivation effect on highly drug-resistant wound bacteria. First, the power supply was set to other operating modes to process 100 ml of physiological saline. The power output parameters were set to default mode (pulse frequency 1 kHz, pulse width 1 μs), output voltage 7 kV, and PAW preparation time 10 min. The bactericidal effect on Pseudomonas aeruginosa was as follows: Figure 7 As shown, PAW has an excellent inactivation effect on Pseudomonas aeruginosa. Treatment for 2 minutes can significantly reduce the number of Pseudomonas aeruginosa, and after 6 minutes of activation, it can basically achieve complete elimination.

[0065] Next, the inactivation effect of PAW on Vibrio vulnificus and Vibrio parahaemolyticus was tested. Activated water was prepared using the same voltage parameters as above, resulting in a total of 8 groups of PAW with identical parameters. Vibrio vulnificus and Vibrio parahaemolyticus were inoculated into 5 culture dishes, with one control group (without PAW treatment) and four experimental groups for each type of Vibrio. The experimental groups were treated with freshly prepared PAW, and PAW that was left to stand for 30 minutes, 60 minutes, and 120 minutes, respectively. The control group was treated with sterile physiological saline. The changes in Vibrio counts in each experimental and control group were recorded at regular intervals to analyze the difference in inhibitory effects of PAW standing time on the two types of Vibrio. The experimental results are as follows: Figure 8 As shown, where Figure 8 (a) Schematic diagram of the bactericidal rate of PAW against Vibrio vulnificus at different placement and treatment times. Figure 8 (b) is a schematic diagram of the bacterial count of Vibrio parahaemolyticus by PAW at different storage and treatment times.

[0066] Using freshly prepared PAW, after 5 min of treatment, the number of Vibrio vulnificus colonies decreased from 6.85 Log10 CFU / mL to 6.1 Log10 CFU / mL, and the number of Vibrio parahaemolyticus colonies decreased from 6.2 Log10 CFU / mL to 5.6 Log10 CFU / mL. After 20 min of treatment, the number of Vibrio vulnificus colonies decreased to 4.0 Log10 CFU / mL, and the number of Vibrio parahaemolyticus colonies decreased to 3.62 Log10 CFU / mL. Using PAW (polydioxanone) that had been left to stand for 120 min, after 5 min of treatment, the colony count of Vibrio vulnificus decreased from 6.85 Log10 CFU / mL to 6.55 Log10 CFU / mL, and the colony count of Vibrio parahaemolyticus decreased from 6.2 Log10 CFU / mL to 6.09 Log10 CFU / mL. After 20 min of treatment, the colony count of Vibrio vulnificus decreased to 5.82 Log10 CFU / mL, and the colony count of Vibrio parahaemolyticus decreased to 5.46 Log10 CFU / mL. It can be concluded that, regardless of the type of bacteria, freshly prepared PAW has the best bactericidal effect. The bactericidal activity gradually decreases with prolonged standing time; that is, the bactericidal activity of PAW decays with standing time, but it still retains a bactericidal effect.

[0067] This invention features a highly integrated design, a compact and lightweight structure, and is easy to carry and use. It achieves a breakthrough by efficiently generating plasma-activated water rich in active substances at room temperature and pressure, making it particularly suitable for various applications such as bedside treatment, outdoor emergency wound care, and daily home care. This portable design not only significantly improves the device's applicability but also dramatically enhances the efficiency of wound infection control and treatment effectiveness, providing a completely new solution for medical care.

[0068] Although the present invention has been disclosed above with reference to preferred embodiments, these are not intended to limit the invention. Any person skilled in the art can make various changes or modifications without departing from the spirit and scope of the invention. Therefore, the scope of protection of the present invention should be defined by the scope of the claims of this application.

Claims

1. A ready-to-use plasma-activated water wound cleaning and treatment device, comprising a housing, characterized in that: The power module is located inside the enclosure, and the electrode assembly is integrated inside the power module. The electrode assembly includes a ground electrode (1) and a high-voltage electrode. The ground electrode (1) is a woven metal mesh bandage structure. The high-voltage electrode is wrapped by a quartz tube (7) with breathable micropores at the bottom. The quartz tube (7) is connected to the air pump inside the enclosure through an air pipe. The high-voltage electrode is connected to the power module through a high-voltage wire.

2. The ready-to-use plasma-activated water wound cleaning and treatment device as described in claim 1, characterized in that: The power module includes a self-dielectric DBD micropore bubble discharge electrode, an air pump unit, a DC bus control unit controlled by an STM32 CNC unit, a human-machine interaction control unit, and a PWM generation unit. The DC bus control unit controls the AC-DC programmable bus power unit to output DC power to the Marx main power circuit unit through electrical connection lines. The Marx main power circuit unit supplies the high voltage required by the high voltage electrode through a high voltage line. At the same time, the air pump unit introduces air to the quartz tube (7). A magnetic isolation drive unit and a PWM generation unit are also provided and connected to the Marx main power module to generate control signals to the magnetic ring primary side of the Marx main power module.

3. The ready-to-use plasma-activated water wound cleaning and treatment device as described in claim 2, characterized in that: The magnetic isolation drive unit uses a single insulated wire to directly pass through the nano-amorphous magnetic ring to form the primary side circuit, and the secondary side uses a 3-turn winding. The magnetic isolation drive unit includes two high-speed switching transistors. The primary side uses a half-bridge circuit structure as the excitation source, and its alternating conduction is controlled by the front-stage PWM signal.

4. The ready-to-use plasma-activated water wound cleaning and treatment device as described in claim 1, characterized in that: The high-voltage electrode is a metal needle, and the metal needle has a high-voltage electrode shell (6) on the outside of the quartz tube (7). One end of the metal needle is connected to a high-voltage wire.

5. The ready-to-use plasma-activated water wound cleaning and treatment device as described in claim 1, characterized in that: The quartz tube (7) is provided with a protective sleeve (8).

6. The ready-to-use plasma-activated water wound cleaning and treatment device as described in any one of claims 1-5, characterized in that: A hollow flexible tube is provided, and the air pipe and high-voltage wire are installed inside the hollow flexible tube, forming an air pipe and high-voltage wire assembly (5).

7. The ready-to-use plasma-activated water wound cleaning and treatment device as described in any one of claims 1-5, characterized in that: The box body is provided with a mechanical button control panel (2), and the mechanical button control panel is provided with a mechanical knob (3).

8. The ready-to-use plasma-activated water wound cleaning and treatment device as described in claim 7, characterized in that: It also includes an HMI human-machine interface console (4), which integrates a touch screen display. Users can directly select the required preparation parameters on the screen, including discharge time, discharge voltage, water volume and pulse parameters, and the current operating status and remaining time information are displayed on the screen simultaneously.

9. A method for on-demand plasma-activated water wound cleaning, using the on-demand plasma-activated water wound cleaning device as described in any one of claims 1-8, characterized in that: Includes the following steps: Step S01: Remove the ground electrode (1), electrode structure air tube, high voltage wire, high voltage electrode, high voltage electrode shell (6), quartz tube (7) and protective sleeve (8), and pull out the protective sleeve (8). Step S02: Connect the mains power socket (9) to power; Step S03: The ground electrode (1) is a woven metal mesh bandage structure that wraps the treatment solution bottle and serves as the dielectric layer in the DBD mode; Step S04: Turn on the air pump to continuously introduce air into the quartz glass tube, insert the quartz tube (7) wrapped with the high voltage electrode into the solution, and generate microbubbles in the solution; select the corresponding solution specification on the HMI human-machine interface (4), then input the voltage parameters, and then start the device on the screen to begin the preparation and activation; Step S05: The preparation will stop automatically after the preparation time is over. The activated water preparation is complete. Take the prepared activated water and wipe it on the wound.

10. The method for treating wounds with plasma-activated water as described in claim 9, characterized in that: In step S04, the voltage and current parameters of the input DC power are observed through the mechanical operating panel (2). If the screen is interrupted and cannot be operated, the voltage is adjusted and the power is cut off in an emergency by using the mechanical button (3) on the mechanical operating panel (2).

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