Betel nut liquid fertilizer for promoting root growth and enhancing disease resistance and preparation method and application thereof

Abstract

The application discloses a liquid fertilizer for areca nut, which promotes root growth and enhances disease resistance, and a preparation method and application thereof, and belongs to the technical field of fertilizers. The fertilizer is composed of a complex rooting agent of indole lactic acid 0.1-0.5 g / L, 4-amino-5-imidazole formamide 0.1-0.2 g / L and polyaspartic acid 5.0-6.0 g / L, and is compounded with bacillus velezensis and combined with corresponding nutrient elements according to the nutrient requirements of the areca nut, so that the components play a synergistic role. Experimental results show that the liquid fertilizer can effectively promote the growth of the root system of the areca nut, improve the yield of the areca nut, reduce the harm of the areca nut yellowing disease, and improve the content of soil organic matter without causing burden to the soil. Meanwhile, the preparation method of the fertilizer is simple, convenient to operate, and suitable for large-scale production and popularization and application.

Description

Technical Field

[0001] This invention belongs to the field of fertilizer technology, specifically relating to a liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance, as well as its preparation method and application. Background Technology

[0002] areca nut ( Areca catechu Areca catechu (L.) is an evergreen tree belonging to the genus Areca in the family Arecaceae, order Arecaceae, class Magnoliopsida. It is one of the four major southern Chinese medicinal herbs and an important tropical economic crop in Hainan and other regions, possessing high economic and medicinal value. During areca cultivation, problems such as slow root growth, soil acidification and compaction, and frequent diseases severely affect the yield and quality of areca nuts.

[0003] Currently available areca nut fertilizers, as well as those reported in some literature, often only provide a single nutrient element and cannot simultaneously meet the needs of promoting root growth and enhancing disease resistance. While some conventional fertilizers claim to have certain root-promoting or disease-resistant effects, their actual application in areca nut cultivation is not ideal, failing to fundamentally solve the problems of root growth and disease resistance faced in areca nut planting. Therefore, developing a fertilizer suitable for areca nut growth and capable of effectively enhancing disease resistance would significantly promote the development of the areca nut industry. Summary of the Invention

[0004] To address the aforementioned research deficiencies, this invention provides a liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance, along with its preparation method and applications.

[0005] The technical solution of this invention mainly includes the following:

[0006] A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance, comprising the following components: Component 1, Component 2, and Component 3;

[0007] Component 1: Indolelactic acid 0.1~0.5 g / L, 4-amino-5-imidazolium carboxamide 0.1~0.2 g / L and polyaspartic acid 5.0~8.0 g / L;

[0008] Component 2: Bacillus belye;

[0009] Component 3: Fish peptides, brown sugar, potassium dihydrogen phosphate, potassium nitrate, urea, magnesium sulfate, calcium carbonate, and trace elements.

[0010] Preferably, the contents of each component in component three in the areca nut liquid fertilizer are as follows: fish peptide 40%–50% (v / v), brown sugar 10–20 g / L, potassium dihydrogen phosphate 60.0 g / L, potassium nitrate 120.0 g / L, urea 30.0 g / L, magnesium sulfate 30.0 g / L, calcium carbonate 30.0 g / L, and trace elements 18.0 g / L.

[0011] Preferably, the trace elements are borax, zinc sulfate, copper sulfate pentahydrate, ferrous sulfate heptahydrate, and manganese chloride tetrahydrate.

[0012] Preferably, the mass ratio of borax, zinc sulfate, copper sulfate pentahydrate, ferrous sulfate heptahydrate, and manganese chloride tetrahydrate among the trace elements is 10:2:3:2:1.

[0013] Preferably, the effective viable count of Bacillus vesiculosus in the areca nut liquid fertilizer is ≥ 5 billion CFU / mL.

[0014] This invention also provides a method for preparing the areca nut liquid fertilizer, which includes the following steps: measuring fish peptides, adding trace elements, and mixing well; then sequentially adding polyaspartic acid and a combination of 4-amino-5-imidazolium carboxamide and indolelactic acid, and mixing well; then adding brown sugar, potassium dihydrogen phosphate, potassium nitrate, urea, magnesium sulfate, and calcium carbonate, and adding water to dissolve as much as possible; finally, adding Bacillus belye and adding water to make up the volume.

[0015] On the other hand, the present invention provides the application of the aforementioned areca nut liquid fertilizer in promoting areca nut root growth and / or improving areca nut disease resistance.

[0016] Preferably, promoting areca nut root growth includes promoting areca nut root division and / or promoting areca nut root elongation.

[0017] Preferably, the application step is as follows: diluting the areca liquid fertilizer and then spraying or dripping it onto the areca plants.

[0018] Preferably, the disease resistance includes resistance to areca nut yellowing disease.

[0019] The beneficial effects of this invention are:

[0020] This invention uses relatively mild indole-lactic acid (compared to indole-acetic acid) as a root promoter, combined with Bacillus belyi, and promotes root growth through the synergistic effect of 4-amino-5-imidazolium carboxamide and polyaspartic acid (PASP). At the same time, it adds organic matter such as fish peptides and brown sugar, as well as inorganic nutrients required by areca nut in a specific ratio, thereby simultaneously achieving multiple effects such as promoting areca nut root growth, increasing yield, reducing areca nut diseases, and increasing soil organic matter content.

[0021] Specifically, the present invention has the following advantages:

[0022] 1) The compound formula provided by this invention is beneficial to promoting the growth of areca nut roots, promoting root division and root elongation.

[0023] 2) The compound formula provided by this invention is beneficial in alleviating areca nut yellowing disease.

[0024] 3) The composite formulation provided by this invention is beneficial to improving photosynthetic efficiency.

[0025] 4) This invention formulates a special formula for areca nuts based on their nutritional needs during the growth process. It not only promotes root growth but also provides precise nutrients, improves soil microbial ecology, increases soil organic matter, and does not burden the soil.

[0026] 5) The preparation method provided by this invention is simple, easy to operate, and suitable for large-scale production and use. Detailed Implementation

[0027] To facilitate a better understanding of the technical content of this invention by those skilled in the art, the invention will be further described in detail below through specific embodiments. Unless otherwise specified, any reagents, pharmaceuticals, or bacterial agents involved in the following embodiments are commercially available products.

[0028] In the following examples or comparative examples, the fish peptides are fish peptide stock solutions prepared using conventional methods, namely, stock solutions obtained by enzymatic hydrolysis of pure fish followed by filtration and then bacterial hydrolysis. Specific steps include: cleaning the whole fish, crushing it into a slurry, heating the fish slurry to 55°C and adjusting the pH to neutral, adding trypsin at 0.02% by mass, and hydrolyzing for approximately 7-8 hours. After hydrolysis, adding Bifidobacterium and Lactobacillus at 0.02% by mass respectively, stirring and fermenting for 7-10 days, aerating for 1 hour daily, to obtain the fish peptide stock solution.

[0029] Example 1

[0030] A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance is prepared by the following steps:

[0031] (1) Measure 500 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0032] (2) Add PASP 5.0 g, 4-amino-5-imidazolium carboxamide 0.1 g and ILA 0.1 g in sequence and mix well.

[0033] (3) Add 10 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0034] (4) Add Bacillus belysinus, mix well, and add water to make up to 1 liter so that the effective viable count is ≥ 5 billion CFU / mL.

[0035] Example 2

[0036] A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance is prepared by the following steps:

[0037] (1) Measure 500 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0038] (2) Add PASP 5.0 g, 4-amino-5-imidazolium carboxamide 0.1 g and ILA 0.5 g in sequence and mix well.

[0039] (3) Add 10 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0040] (4) Add Bacillus belysinus, mix well, and add water to make up to 1 liter so that the effective viable count is ≥ 5 billion CFU / mL.

[0041] Example 3

[0042] A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance is prepared by the following steps:

[0043] (1) Measure 400 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0044] (2) Add PASP 8.0g, 4-amino-5-imidazolium carboxamide 0.2g and ILA 0.1g in sequence and mix well.

[0045] (3) Add 20 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0046] (4) Add Bacillus belysinus, mix well, and add water to make up to 1 liter so that the effective viable count is ≥ 5 billion CFU / mL.

[0047] Comparative Example 1

[0048] A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance (ILA-free) is prepared as follows:

[0049] (1) Measure 500 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0050] (2) Add 5.0 g of PASP and 0.1 g of 4-amino-5-imidazolium carboxamide in sequence and mix well.

[0051] (3) Add 10 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0052] (4) Add Bacillus belysinus, mix well, and add water to make up to 1 liter so that the effective viable count is ≥ 5 billion CFU / mL.

[0053] Comparative Example 2

[0054] A liquid fertilizer for areca nut that promotes root growth and enhances disease resistance (excluding the combination of PASP and 4-amino-5-imidazolium carboxamide) is prepared as follows:

[0055] (1) Measure 500 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0056] (2) Add 0.5 g of ILA and mix well.

[0057] (3) Add 10 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0058] (4) Add Bacillus belysinus, mix well, and add water to make up to 1 liter so that the effective viable count is ≥ 5 billion CFU / mL.

[0059] Comparative Example 3

[0060] A liquid fertilizer for areca nut that promotes root growth and enhances disease resistance (using carbendazim instead of 4-amino-5-imidazolium carbendazim) is prepared as follows:

[0061] (1) Measure 500 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0062] (2) Add PASP 5.0 g, carbendazim (N-(2-benzimidazolyl)-methyl carbamate) 0.1 g and ILA 0.5 g in sequence, and mix well.

[0063] (3) Add 10 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0064] (4) Add Bacillus belysinus, mix well, and add water to make up to 1 liter so that the effective viable count is ≥ 5 billion CFU / mL.

[0065] Comparative Example 4

[0066] A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance (without Bacillus vesiculosus) is prepared as follows:

[0067] (1) Measure 500 mL of fish peptides, add 10.0 g of borax, 2.0 g of zinc sulfate, 3.0 g of copper sulfate pentahydrate, 2.0 g of ferrous sulfate heptahydrate and 1.0 g of manganese chloride tetrahydrate, and mix well.

[0068] (2) Add PASP 5.0 g, 4-amino-5-imidazolium carboxamide 0.1 g and ILA 0.5 g in sequence and mix well.

[0069] (3) Add 10 g of brown sugar, 60.0 g of potassium dihydrogen phosphate, 120.0 g of potassium nitrate, 30.0 g of urea, 30.0 g of magnesium sulfate and 30.0 g of calcium carbonate, mix well and add water to dissolve as much as possible.

[0070] (4) Add water to make up to 1 liter.

[0071] Comparative Example 5

[0072] Conventional water-soluble fertilizer containing macro-elements (liquid, macro-elements ≥400g / L, N:P2O5:K2O ratio 1:1:1).

[0073] Experimental Example: Fertilization Verification Experiment for Areca Nut

[0074] The areca nut liquid fertilizer prepared in Examples 1, 2, 1, 2, 3, and 4, and the water-soluble fertilizer containing macro-elements in Comparative Example 5 were used as test subjects.

[0075] In a betel nut plantation in Chengxi Village, Yazhou District, Chengmai County, Hainan Province, where plants with moderate yellowing disease (45% incidence rate) had been growing for 7 years and exhibited similar growth patterns, the above-mentioned fertilizer was diluted with water 300-500 times and applied via sprinkler or drip irrigation at a rate of 5 liters per mu (approximately 0.067 hectares), once a month, while normal orchard management was maintained. Each treatment consisted of 50 plants, and various indicators were statistically analyzed after one year of fertilization. The indicators for each treatment are shown in Table 3.

[0076] The incidence rate of areca nut yellowing disease is calculated as follows:

[0077] Incidence rate (%) = (Total number of plants - Number of healthy plants) / (Total number of plants surveyed) × 100

[0078] The method for calculating the disease index of areca nut yellowing is as follows:

[0079] Disease index = 100 × [∑(number of diseased plants at each level × representative value of severity at each level) / (total number of plants surveyed × highest representative value)];

[0080] Chlorophyll content determination method: The chlorophyll content of the third leaf was measured using a handheld chlorophyll content meter (model: SPAD-502, Zhejiang Top Instrument Co., Ltd.).

[0081] Table 1 Disease Classification

[0082]

[0083] Table 2 Classification Standards for the Severity of Areca Chloride Yellowing Disease

[0084]

[0085] Table 3. Corresponding indicators after different treatments

[0086]

[0087] The corresponding indicators in Example 3 are similar to those in Example 1 and Example 2, with no significant differences. The data are omitted.

[0088] Based on the results of the examples and comparative examples in Table 3, it can be seen that the areca nut yields of Examples 1 and 2 reached 24.6~25.3 kg / plant, while the yields of Comparative Examples 1 to 5 were 20.8, 19.3, 21.5, 18.6, and 17.0 kg / plant, respectively, showing a significant difference from the examples. Furthermore, the number of areca nut roots (250~310) and root length (1.52~1.61 cm) in the examples were much higher than those in the comparative examples (the maximum number of roots was only 200, and the longest root length was only 1.35 cm). This indicates that the synergistic effect of ILA, 4-amino-5-imidazolium carboxamide, PASP, and Bacillus belyssus can create a positive cycle from root development, nutrient absorption, and increased yield.

[0089] The chlorophyll content of areca nut leaves in the embodiment (37.9%–38.5%) was significantly higher than that in the comparative example (maximum only 33.8%), indicating that the compound formula can promote chlorophyll synthesis, improve photosynthetic efficiency, and provide an energy basis for growth and disease resistance. The chlorosis disease severity index (0.31–0.35) and incidence rate (46%–47%) in the embodiment were significantly lower than those in the comparative example (maximum severity index 0.55, maximum incidence rate 75%), proving that the areca nut liquid fertilizer of the present invention can effectively inhibit the deterioration of chlorosis and reduce disease.

[0090] Furthermore, the soil organic matter content in the embodiment was stable at 16.5 g / kg, and the pH was maintained at 6.6~6.7 (a slightly acidic environment suitable for areca nut growth); while the soil organic matter content in the comparative example was as low as 14.8 g / kg, and the pH dropped to as low as 5.3 (excessive acidity can easily aggravate chlorosis). It can be seen that the liquid fertilizer in the embodiment of the present invention can play a role in improving the soil.

[0091] Comparative Examples 1, 2, 4, and 5 significantly lagged behind the Example in all indicators, including root system performance, yield, disease resistance, and soil conditions. This fully demonstrates the irreplaceable synergistic effect among ILA, 4-amino-5-imidazolium carbendazim, PASP, and Bacillus belyssus, with each component exhibiting complementary functions in root development, nutrient utilization, disease resistance, and soil improvement. Replacing 4-amino-5-imidazolium carbendazim with other imidazole derivatives (such as carbendazim, Comparative Example 3) also failed to achieve the desired results.

[0092] In summary, this invention achieves comprehensive optimization of the entire chain of areca nut root growth, disease resistance, and soil improvement through precise synergy of multiple components.

[0093] The embodiments described above are only some embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention shall fall within the protection scope of the present invention.

Claims

1. A liquid fertilizer for areca nuts that promotes root growth and enhances disease resistance, characterized in that, It includes the following components: Component 1, Component 2, and Component 3; Component 1: Indolelactic acid 0.1~0.5 g / L, 4-amino-5-imidazolium carboxamide 0.1~0.2 g / L and polyaspartic acid 5.0~8.0 g / L; Component 2: Bacillus belyi; the effective viable count of Bacillus belyi in the areca nut liquid fertilizer is ≥ 5 billion CFU / mL; Component 3: Fish peptides 40%–50% (v / v), brown sugar 10–20 g / L, potassium dihydrogen phosphate 60.0 g / L, potassium nitrate 120.0 g / L, urea 30.0 g / L, magnesium sulfate 30.0 g / L, calcium carbonate 30.0 g / L, and trace elements 18.0 g / L.

2. The areca nut liquid fertilizer according to claim 1, characterized in that, The trace elements are borax, zinc sulfate, copper sulfate pentahydrate, ferrous sulfate heptahydrate, and manganese chloride tetrahydrate.

3. The areca nut liquid fertilizer according to claim 2, characterized in that, The mass ratio of the trace elements borax, zinc sulfate, copper sulfate pentahydrate, ferrous sulfate heptahydrate, and manganese chloride tetrahydrate is 10:2:3:2:

1.

4. The method for preparing areca nut liquid fertilizer according to any one of claims 1 to 3, characterized in that, Includes the following steps: Measure out the fish peptides, add trace elements, and mix well; then add polyaspartic acid, 4-amino-5-imidazolium carboxamide, and indolelactic acid in sequence, and mix well; then add brown sugar, potassium dihydrogen phosphate, potassium nitrate, urea, magnesium sulfate, and calcium carbonate, and add water to dissolve as much as possible; finally, add Bacillus belye and add water to make up to the final volume.

5. The use of the areca nut liquid fertilizer according to any one of claims 1 to 3 in promoting areca nut root growth and / or improving areca nut disease resistance.

6. The application according to claim 5, characterized in that, The promotion of areca nut root growth includes promoting root division and / or promoting root elongation.

7. The application according to claim 5, characterized in that, The steps for the application are as follows: The areca nut liquid fertilizer is diluted and then sprayed or dripped onto the areca nut plants.

8. The application according to claim 5, characterized in that, The disease resistance includes resistance to areca nut yellowing disease.

Images