Bacteriostatic deodorization composition, preparation method thereof and bacteriostatic deodorant

Abstract

The invention discloses a bacteriostasis and deodorization composition, a preparation method thereof and a bacteriostasis and deodorization agent, the bacteriostasis and deodorization composition comprises a plant extract and a composite biological enzyme preparation, and the mass ratio of the plant extract to the composite biological enzyme preparation is 1: (0.1-0.5); the composite biological enzyme preparation comprises at least one plant enzyme and at least one animal enzyme, and the mass ratio of the plant enzyme to the animal enzyme is 1: (0.1-0.7). By implementing the cleaning agent, the effect of removing peculiar smell is achieved while cleaning and antibiosis are achieved, and no harm is caused to human beings and pets.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bacteriostatic deodorant preparations, and particularly relates to a bacteriostatic deodorant composition, a preparation method thereof and a bacteriostatic deodorant. BACKGROUND

[0002] The individual odor of pets mainly comes from odor glands, saliva, urine and feces, etc., and the contact with bacteria, parasites and viruses directly affects the formation of odor substances, so that pets produce odor, and the parts that often produce odor include oral cavity, gastrointestinal tract, skin, etc. The physical deodorant removes odor through physical adsorption of porous substances, and the adsorption time is long and the deodorization speed is slow. The chemical deodorant converts odor components into odorless substances through oxidation, reduction, addition, condensation and other chemical reactions, but the chemical components should be carefully selected to avoid harming the health of humans and pets. The biological deodorant degrades common odor factors by using microbial flora metabolism or its metabolites. The plant deodorant generates odorless and non-toxic substances by extracting effective components of plants and chemically reacting with odor molecule groups. In addition, pets are more sensitive to odor than humans, and considering their licking habit, the safety requirements for related products are higher. SUMMARY

[0003] The technical problem to be solved by the present application is to provide a bacteriostatic deodorant composition and a preparation method thereof, which can remove odor while cleaning and antibacterial, and will not harm humans and pets.

[0004] The technical problem to be solved by the present application is to provide a bacteriostatic deodorant composition and a preparation method thereof, which can remove odor while cleaning and antibacterial, and will not harm humans and pets.

[0005] In order to solve the above problems, the present application discloses a bacteriostatic deodorant composition, which comprises a plant extract and a composite biological enzyme preparation, and the mass ratio of the plant extract and the composite biological enzyme preparation is 1:(0.1-0.5); the composite biological enzyme preparation comprises at least one plant enzyme and at least one animal enzyme, and the mass ratio of the plant enzyme and the animal enzyme is 1:(0.1-0.7).

[0006] As an improvement of the above-mentioned scheme, the plant enzyme comprises one or more of bromelain, papain, horseradish peroxidase, pectinase and laccase;

[0007] The animal enzyme comprises one or more of egg white lysozyme, pepsin and trypsin;

[0008] The activity of the composite biological enzyme preparation is 1500 U / g-5000 U / g.

[0009] As the improvement of the above-mentioned scheme, the complex biological enzyme preparation comprises bromelain and egg white lysozyme, and the mass ratio of the bromelain and the egg white lysozyme is 1:(0.2-0.5).

[0010] As the improvement of the above-mentioned scheme, the activity of the bromelain is 1500U / g-2500U / g, and the activity of the egg white lysozyme is 3500U / g-5000U / g.

[0011] As the improvement of the above-mentioned scheme, the complex biological enzyme preparation is a fixed complex biological enzyme preparation; the fixed enzyme carrier is modified starch.

[0012] The modified starch comprises cationic starch and oxidized starch, the cationic starch is used for fixing the bromelain, and the oxidized starch is used for fixing the egg white lysozyme.

[0013] As the improvement of the above-mentioned scheme, the plant extract comprises chamomile extract, peony extract and lavender extract, and the mass ratio of the chamomile extract, the peony extract and the lavender extract is 1:(0.5-1):(0.8-1.2).

[0014] As the improvement of the above-mentioned scheme, the chamomile extract is obtained by extracting 50wt%-70wt% of ethanol aqueous solution at 60°C-65°C for 1.5h-2.5h;

[0015] The peony extract is obtained by extracting 50wt%-60wt% of ethanol aqueous solution at 70°C-75°C for 1.5h-2h;

[0016] The lavender extract is obtained by extracting 65wt%-75wt% of ethanol aqueous solution at 50°C-55°C for 1h-1.5h.

[0017] Correspondingly, the application further discloses a preparation method of the bacteriostatic and deodorant composition.

[0018] Correspondingly, the application further discloses a bacteriostatic and deodorant agent comprising the bacteriostatic and deodorant composition.

[0019] The bacteriostatic and deodorant agent is in a liquid dosage form, and further comprises a solvent; the solvent comprises a surfactant and deionized water, and the mass ratio of the bacteriostatic and deodorant composition and the buffer protection system is 1:(5-10).

[0020] The antibacterial and deodorizing agent is in solid form, and the antibacterial and deodorizing agent also includes a carrier; the carrier is one or more of activated carbon, zeolite, diatomaceous earth, activated clay, bentonite, and white carbon black, and the mass ratio of the antibacterial and deodorizing composition to the carrier is 1:(3-5).

[0021] Accordingly, the present invention also discloses the application of the above-mentioned antibacterial and deodorizing agent in pet deodorizing products.

[0022] Implementing this invention has the following beneficial effects:

[0023] The antibacterial and deodorizing composition provided by this invention includes a compound biological enzyme preparation composed of at least one plant enzyme and at least one animal enzyme. The animal enzyme directly destroys the bacterial cell wall, while the plant enzyme targets and decomposes odor substances. It has a fast onset of action and a wide range of effects. The compound biological enzyme preparation is non-toxic and harmless. It works synergistically with plant extracts to effectively remove odors while being more easily accepted by pets, and has no side effects on pets or humans. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in further detail below.

[0025] This invention provides an antibacterial and deodorizing composition comprising plant extracts and a compound bio-enzyme preparation, wherein the mass ratio of the plant extracts to the compound bio-enzyme preparation is 1:(0.1-0.5); the compound bio-enzyme preparation comprises at least one plant enzyme and at least one animal enzyme, wherein the mass ratio of the plant enzyme to the animal enzyme is 1:(0.1-0.7). Preferably, the mass ratio of the plant enzyme to the animal enzyme is 1:(0.4-0.7).

[0026] The antibacterial and deodorizing composition provided by this invention includes a compound biological enzyme preparation composed of at least one plant enzyme and at least one animal enzyme. The animal enzyme directly destroys the bacterial cell wall, while the plant enzyme targets and decomposes odor substances. It has a fast onset of action and a wide range of effects. The compound biological enzyme preparation is non-toxic and harmless. It works synergistically with plant extracts to effectively remove odors while being more easily accepted by pets, and has no side effects on pets or humans.

[0027] Plant extracts contain chemical substances similar to catechol, hydroquinone, or resorcinol. When they come into full contact with odor molecules, they can undergo chemical reactions such as decomposition, polymerization, substitution, replacement, and addition, weakening the chemical bonds in the odor molecules and changing their original molecular structure to generate non-toxic and odorless substances. In particular, they can combine with ammonia compounds and / or sulfur compounds to generate odorless substances. They also have a certain odor masking effect, are relatively safe, and can instantly cover odors, providing good immediate odor removal.

[0028] In an embodiment, the plant extracts include chamomile extract, peony extract, and lavender extract, and the mass ratio of the chamomile extract, the peony extract, and the lavender extract is 1:(0.5-1):(0.8-1.2), for example, 1:0.6:0.9, 1:0.5:1, 1:1:1, 1:0.8:0.8, or 1:0.6:1.1, but not limited thereto.

[0029] The chamomile extract mainly has antibacterial and antioxidant effects. The polyphenols, flavonoids, and cyclic ether compounds in the chamomile extract have significant antibacterial activity and can effectively inhibit the growth of common pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. In addition, the chamomile extract also has strong antioxidant activity and can scavenge free radicals and protect cells from oxidative damage. The chamomile extract can also reduce the odor on the surface of the pet and has a calming effect on the nervous system of the pet, which helps to relieve anxiety and tension.

[0030] The peony extract mainly has antibacterial and anti-inflammatory effects. Paeoniflorin is the main active ingredient, and it has good bacteriostatic effect on bacterial skin diseases. In addition, the peony extract can promote skin repair while inhibiting bacterial growth, reducing the risk of pet skin infection.

[0031] The lavender extract mainly has antibacterial, antioxidant, and calming effects. The sesquiterpenes, phenols, and ester compounds in the lavender extract have significant antibacterial activity and can effectively inhibit the growth of common pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. In addition, the lavender extract also has good antioxidant activity and can scavenge free radicals and protect cells from oxidative damage. The lavender extract can also help pets relax and relieve anxiety, thereby improving the overall health of the pet.

[0032] The chamomile extract, the peony extract, and the lavender extract are used synergistically to enhance the antibacterial effect. The chamomile extract and the lavender extract mainly inhibit the growth of bacteria to achieve antibacterial effect, and the peony extract inhibits the adhesion of bacteria and destroys their structure to achieve antibacterial effect. The synergistic use of the three can more comprehensively cover a variety of pathogenic bacteria and improve the overall antibacterial effect. In addition, the synergistic use of the three can further enhance the antioxidant effect and reduce the oxidative stress reaction on the surface of the pet, thereby improving the skin health. The chamomile and lavender extracts with calming and soothing effects can provide immediate fragrance masking and help pets relieve anxiety and tension.

[0033] In one embodiment, the chamomile extract is obtained by extracting the chamomile dry powder with 50wt%-70wt% ethanol aqueous solution at 60-65°C for 1.5-2.5h. In a preferred embodiment, the chamomile extract comprises a first extract and a second extract, specifically, the chamomile dry powder is extracted with 50wt%-60wt% ethanol aqueous solution at 60-65°C for 1-1.5h to obtain the first extract and the residue, and the residue is extracted with 60wt%-70wt% ethanol aqueous solution at 60-65°C for 0.5-1h to obtain the second extract, so that the flavonoids and terpenes are enriched in different stages, and the content of the effective components in the chamomile extract is increased.

[0034] In one embodiment, the peony extract is obtained by extracting the peony dry powder with 50wt%-60wt% ethanol aqueous solution at 70-75°C for 1.5-2h.

[0035] In one embodiment, the lavender extract is obtained by extracting the lavender dry powder with 65wt%-75wt% ethanol aqueous solution at 50-55°C for 1-1.5h.

[0036] By limiting the extraction method, the content of the effective components in the plant extract is increased, and the effect of the plant extract is significantly improved.

[0037] The composite biological enzyme preparation can exert the effects of deodorization and bacteriostasis. Among them, the plant enzyme can quickly decompose odor molecules, and the animal enzyme can further decompose residual protein and fat substances, so as to realize more thorough deodorization effect. In addition, the plant enzyme can also act as an auxiliary component of the animal enzyme to improve its stability and activity, so that it still maintains good catalytic effect under different environmental conditions. In order to ensure the effect of the composite biological enzyme preparation, the activity of the plant enzyme and the animal enzyme should be greater than 1000U / g, and in one embodiment, the activity of the plant enzyme and the animal enzyme is 1500U / g-5000U / g.

[0038] Optionally, the plant enzyme comprises one or more of bromelain, papain, horseradish peroxidase, pectinase, and laccase. The plant enzyme can hydrolyze and cut the sulfur bond in the odor molecules, and also can catalyze the polymerization of phenols and sulfides into odorless macromolecules, realizing long-acting deodorization.

[0039] Optionally, the animal enzyme includes one or more of egg white lysozyme, pepsin, and trypsin. The animal enzyme can hydrolyze bacterial cell walls, has a targeted bactericidal function, and kills odor-producing bacteria. The plant extract, plant enzyme, and animal enzyme work synergistically, while improving the removal rate of odor molecules and the bacteriostatic effect. The plant extract deodorizes while creating a suitable environment for the work of the biological enzyme. The plant enzyme pretreats macromolecular malodorous substances, improving the action efficiency of the animal enzyme. The animal enzyme removes pathogenic bacteria. The three work synergistically, achieving rapid deodorization and ensuring long-term stability of the deodorization and bacteriostatic effect.

[0040] In a preferred embodiment, the complex biological enzyme preparation includes bromelain and egg white lysozyme, and the mass ratio of the bromelain to the egg white lysozyme is 1:(0.2-0.5), for example 1:0.25, 1:0.3, 1:0.35, 1:0.4, or 1:0.45, but is not limited thereto. Bromelain hydrolyzes protein odor precursors, decomposing them into small molecular fragments and reducing the generation of mercaptans and ammonia. Egg white lysozyme directly damages bacterial cell walls, cutting off the source of microbial metabolism and odor production. Moreover, the proteolytic action of bromelain can expose the peptidoglycan layer of the bacterial cell wall, significantly enhancing the bactericidal efficiency of the egg white lysozyme. In addition, the mild decomposition characteristics of bromelain can reduce the potential irritation of the egg white lysozyme to pet skin. The complex biological enzyme preparation is natural, environmentally friendly, and has both safety and high efficiency.

[0041] In an embodiment, the activity of the bromelain is 1500 U / g-2500 U / g, and the activity of the egg white lysozyme is 3500 U / g-5000 U / g. By controlling the activity of the bromelain and the egg white lysozyme, the efficacy and safety are balanced. Controlling the activity of the egg white lysozyme to be 3500 U / g-5000 U / g can improve the bacteriostatic performance while reducing irritation. Controlling the activity of the bromelain to be 1500 U / g-2500 U / g can ensure the removal rate of ammonia and other odors while reducing the hydrolysis of the egg white lysozyme.

[0042] In a preferred embodiment, the complex biological enzyme preparation is an immobilized complex biological enzyme preparation, and the immobilized enzyme carrier is modified starch. Specifically, the modified starch includes cationic starch and oxidized starch. The cationic starch is used to immobilize the bromelain, and the oxidized starch is used to immobilize the egg white lysozyme. Preferably, the cationic starch is obtained by introducing ammonium groups on cassava starch using a cationic etherifying agent, and the oxidized starch is obtained by oxidizing cassava starch using ethanol-sodium hypochlorite. The bromelain and the egg white lysozyme are immobilized on different immobilized enzyme carriers, further reducing the mutual influence of the bromelain and the egg white lysozyme.

[0043] Specifically, the cationic starch is prepared as follows: spray the cassava starch with a first NaOH solution, and stir for 0.5-1.5 hours to obtain alkali-activated cassava starch; mix a second NaOH solution with an etherifying agent, and then spray the mixture into the alkali-activated cassava starch, and stir for 1.5-2.5 hours to obtain mixed cassava starch; irradiate the mixed cassava starch in a microwave at 400-500 W for 1-4 minutes, and then neutralize to pH 6-8, and then wash, dry, crush, and obtain the cationic starch. The amount of the etherifying agent is 3-5% of the mass of the cassava starch, the amount of NaOH is 3-5% of the mass of the cassava starch, the mass ratio of the first NaOH solution to the second NaOH solution is 1:(0.3-0.6), and the etherifying agent can be 3-chloro-2-hydroxypropyl trimethylammonium chloride.

[0044] The bromelain is immobilized as follows: dissolve the cationic starch in a buffer solution, add the bromelain, continue stirring for 0.3-0.6 hours, adjust the pH to 5-7, rinse, and then dry in a thermostat at 37°C to obtain the immobilized bromelain in powder form. The mass ratio of the bromelain to the cationic starch is 1:(10-20).

[0045] The preparation method of the cationic starch provided by the application can synthesize cationic starch with high degree of substitution and more adsorption sites, and can complete enzyme loading through electrostatic adsorption in 0.3-0.6 hours, so that efficient immobilization of bromelain can be achieved.

[0046] The oxidized starch is prepared as follows: mix the cassava starch with an ethanol-sodium hypochlorite mixed solution, hydrolyze at 40-50°C for 80-100 minutes, neutralize to pH 6-7, filter, and then place the filter cake in an oven at 40-60°C for 20-24 hours to obtain the oxidized starch. The concentration of the ethanol-sodium hypochlorite mixed solution is 8-12%, and the amount of the ethanol-sodium hypochlorite mixed solution is 2-8% of the mass of the cassava starch.

[0047] The egg white lysozyme is immobilized as follows: dissolve the oxidized starch in a buffer solution, add the egg white lysozyme, continue stirring for 0.5-1.5 hours, adjust the pH to 3-4, rinse, and then dry in a thermostat at 37°C to obtain the immobilized egg white lysozyme in powder form. The mass ratio of the egg white lysozyme to the oxidized starch is 1:(10-20).

[0048] The preparation method of the oxidized starch provided by the application can inhibit excessive degradation of the starch while oxidizing the starch, and the obtained oxidized starch has good thermal stability and anti-retrogradation property. The oxidized starch immobilized egg white lysozyme has the advantages of high activity, strong stability, and controllable release.

[0049] Correspondingly, the application further discloses a preparation method of the bacteriostatic and deodorizing composition.

[0050] Compared with directly mixing the plant extract and the composite biological enzyme preparation, dispersing the plant extract with part of the composite biological enzyme preparation can enhance the dispersibility of the plant extract in the system and avoid affecting the performance of the bacteriostatic and deodorizing composition due to the too large or uneven plant extract particles.

[0051] Correspondingly, the application further discloses a bacteriostatic and deodorizing agent comprising the bacteriostatic and deodorizing composition. It can be understood that the bacteriostatic and deodorizing agent can be in a solid dosage form and / or a liquid dosage form. When the bacteriostatic and deodorizing agent is in the liquid dosage form, the bacteriostatic and deodorizing agent further comprises a solvent. The solvent comprises a surfactant and deionized water, and the mass ratio of the bacteriostatic and deodorizing composition to the solvent is 1:(5-10). The surfactant can be alkyl polyglycoside or cocamidopropyl betaine, but is not limited thereto. When the bacteriostatic and deodorizing agent is in the solid dosage form, the bacteriostatic and deodorizing agent further comprises a carrier. The carrier is one or more of activated carbon, zeolite, diatomite, activated white clay, bentonite and white carbon black, and the use amount ratio of the bacteriostatic and deodorizing composition to the carrier is 1:(3-5).

[0052] Correspondingly, the application further discloses an application of the bacteriostatic and deodorizing agent in a pet deodorizing product.

[0053] The application is further described below with specific examples.

[0054] Example 1

[0055] The bacteriostatic and deodorizing composition comprises a plant extract and a composite biological enzyme preparation, the mass ratio of the plant extract to the composite biological enzyme preparation is 1:0.1, the plant extract is tea tree extract, the composite biological enzyme preparation comprises bromelain and egg white lysozyme, the mass ratio of the bromelain to the egg white lysozyme is 1:0.7, the activity of the bromelain is 5000 U / g, and the activity of the egg white lysozyme is 5000 U / g. The tea tree extract is obtained by extracting 60wt% ethanol aqueous solution at 60℃ for 2h.

[0056] Example 2

[0057] The present example provides a bacteriostatic and deodorizing composition, which is different from example 1 in that the plant extract is a chamomile extract, a peony extract and a lavender extract, and the mass ratio of the chamomile extract, the peony extract and the lavender extract is 1:0.6:1. The chamomile extract is obtained by extracting chamomile dried powder with 60wt% ethanol aqueous solution at 60℃ for 2h, the peony extract is obtained by extracting peony dried powder with 55wt% ethanol aqueous solution at 75℃ for 2h, and the lavender extract is obtained by extracting lavender dried powder with 65wt% ethanol aqueous solution at 55℃ for 1h.

[0058] The rest are the same as example 1.

[0059] Example 3

[0060] The present example provides a bacteriostatic and deodorizing composition, which is different from example 2 in that the chamomile extract includes a first extract and a second extract, and the chamomile dried powder is extracted with 60wt% ethanol aqueous solution at 60℃ for 1.5h to obtain the first extract and residue, and the residue is extracted with 65wt% ethanol aqueous solution at 65℃ for 0.5h to obtain the second extract.

[0061] The rest are the same as example 2.

[0062] Example 4

[0063] The present example provides a bacteriostatic and deodorizing composition, which is different from example 3 in that the mass ratio of bromelain and egg white lysozyme is 1:0.4.

[0064] The rest are the same as example 3.

[0065] Example 5

[0066] The present example provides a bacteriostatic and deodorizing composition, which is different from example 4 in that the activity of bromelain is 2000U / g, and the activity of egg white lysozyme is 4000U / g.

[0067] The rest are the same as example 4.

[0068] Example 6

[0069] The present example provides a bacteriostatic and deodorizing composition, which is different from example 5 in that the complex biological enzyme preparation is a fixed complex biological enzyme preparation, the bromelain is fixed by cationic starch, and the egg white lysozyme is fixed by oxidized starch.

[0070] The rest are the same as example 5.

[0071] Comparative example 1

[0072] The present comparative example provides a bacteriostatic and deodorizing composition, which includes a plant extract, and the plant extract is a tea tree extract.

[0073] The rest are the same as Example 1.

[0074] Comparative Example 2

[0075] This comparative example provides a bacteriostatic deodorizing composition including a complex biological enzyme preparation, the complex biological enzyme preparation including bromelain and egg white lysozyme, the mass ratio of the bromelain and the egg white lysozyme being 1:0.4.

[0076] The rest are the same as Example 1.

[0077] Comparative Example 3

[0078] This comparative example provides a bacteriostatic deodorizing composition including a plant extract and a biological enzyme preparation, the mass ratio of the plant extract and the biological enzyme preparation being 1:0.8, the plant extract being a tea tree extract, and the biological enzyme preparation being egg white lysozyme.

[0079] The rest are the same as Example 1.

[0080] The bacteriostatic deodorizing compositions prepared in Examples 1 to 6 and Comparative Examples 1 to 3 were tested.

[0081] (1) NH3 removal effect: 5 g of the bacteriostatic deodorant was added to the bottom of a 2.5 L reagent bottle, 100 μL of an ammonia-ethanol solution with a concentration of 2% was added along the inner wall of the reagent bottle, the bottle opening was immediately covered with plastic wrap, and after standing for 1 h, 100 mL of gas was drawn and the ammonia concentration was measured with an ammonia detection tube. The blank test only added an equal amount of the ammonia-ethanol solution, and the rest of the operations were the same. The experiment was repeated twice, and the average value was taken as the final ammonia concentration, and the removal rate of the bacteriostatic deodorant on ammonia was calculated.

[0082] (2) Bacteriostatic effect: E. coli, S. aureus, and Salmonella, which are common pathogenic bacteria in pet excrement, were used as indicator bacteria. The bacteriostatic deodorizing composition was mixed with samples containing known amounts of E. coli, S. aureus, and Salmonella, respectively, and after 2 h of action, the viable cell counts of E. coli, S. aureus, and Salmonella were tested, and the bacteriostatic rate was calculated.

[0083] The specific results are as follows:

[0084]

[0085]

[0086] Test Example

[0087] The bacteriostatic deodorizing compositions of Example 6 and Comparative Example 1 were used to prepare solid and liquid bacteriostatic deodorants, respectively, as Test Examples 1 to 4.

[0088] Test Example 1

[0089] This test example provides a bacteriostatic deodorant in solid form, which comprises the bacteriostatic deodorant composition prepared in Example 4 and a carrier, the carrier being activated carbon, and the mass ratio of the bacteriostatic deodorant composition to the carrier being 1:5.

[0090] Test Example 2

[0091] This test example provides a bacteriostatic deodorant in liquid form, which comprises the bacteriostatic deodorant composition prepared in Example 4 and a solvent, the solvent being a surfactant and deionized water, and the mass ratio of the bacteriostatic deodorant composition to the solvent being 1:10.

[0092] Test Example 3

[0093] This test example provides a bacteriostatic deodorant in solid form, which comprises the bacteriostatic deodorant composition prepared in Comparative Example 1 and a carrier, the carrier being activated carbon, and the mass ratio of the bacteriostatic deodorant composition to the carrier being 1:5.

[0094] Test Example 4

[0095] This test example provides a bacteriostatic deodorant in liquid form, which comprises the bacteriostatic deodorant composition prepared in Comparative Example 1 and a solvent, the solvent being a surfactant and deionized water, and the mass ratio of the bacteriostatic deodorant composition to the solvent being 1:10.

[0096] The treated cat litter was respectively placed in 4 washed and dried cat litter pots, and an equal amount of fresh cat feces was placed in each cat litter pot. 20 g of the bacteriostatic deodorant of Test Example 1, 30 mL of the bacteriostatic deodorant of Test Example 2, 20 g of the bacteriostatic deodorant of Test Example 3, and 30 mL of the bacteriostatic deodorant of Test Example 4 were respectively placed or sprayed in the 4 cat litter pots. 8 test personnel were organized to conduct sensory olfactory evaluation on each treated cat litter pot on the 1st, 3rd, 5th, and 7th days, and the sensory olfactory evaluation criteria were as follows:

[0097]

[0098]

[0099] The treated cat litter of Test Example 1 to Test Example 4 was subjected to sensory olfactory evaluation, and the specific results were as follows:

[0100] Day 1 Day 3 Day 5 Day 7 Test Example 1 1 1 1 1 Test Example 2 2 1 1 1 Test Example 3 6 5 5 4 Test Example 4 6 6 6 5

[0101] The above describes the preferred embodiments of the application. It should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the application, and these improvements and refinements are also considered within the protection scope of the application.

Claims

1. An antibacterial and deodorizing composition, characterized in that, It includes plant extracts and compound bio-enzyme preparations, wherein the mass ratio of the plant extracts to the compound bio-enzyme preparations is 1:(0.1 to 0.5); the compound bio-enzyme preparations include at least one plant enzyme and at least one animal enzyme, wherein the mass ratio of the plant enzyme to the animal enzymes is 1:(0.1 to 0.7).

2. The antibacterial and deodorizing composition according to claim 1, characterized in that, The plant enzymes include one or more of bromelain, papain, horseradish peroxidase, pectinase, and laccase. The animal enzymes include one or more of egg white lysozyme, pepsin, and trypsin. The activity of the compound bio-enzyme preparation is 1500U / g to 5000U / g.

3. The antibacterial and deodorizing composition as described in claim 2, characterized in that, The compound biological enzyme preparation includes bromelain and egg white lysozyme, and the mass ratio of bromelain to egg white lysozyme is 1:(0.2-0.5).

4. The antibacterial and deodorizing composition as described in claim 3, characterized in that, The activity of the bromelain is 1500 U / g to 2500 U / g, and the activity of the egg white lysozyme is 3500 U / g to 5000 U / g.

5. The antibacterial and deodorizing composition according to claim 3, characterized in that, The composite bio-enzyme preparation is an immobilized composite bio-enzyme preparation; the immobilized enzyme carrier is modified starch. The modified starch comprises cationic starch and oxidized starch, wherein the cationic starch is used to immobilize the bromelain and the oxidized starch is used to immobilize the egg white lysozyme.

6. The antibacterial and deodorizing composition according to claim 1, characterized in that, The plant extracts include chamomile extract, peony extract and lavender extract, wherein the mass ratio of the chamomile extract, peony extract and lavender extract is 1:(0.5-1):(0.8-1.2).

7. The antibacterial and deodorizing composition according to claim 6, characterized in that, The chamomile extract was obtained by extraction with a 50wt%–70wt% aqueous ethanol solution at 60℃–65℃ for 1.5h–2.5h. The peony extract was obtained by extracting a 50wt%–60wt% aqueous ethanol solution at 70℃–75℃ for 1.5h–2h. The lavender extract was obtained by extracting 65wt% to 75wt% ethanol aqueous solution at 50℃ to 55℃ for 1h to 1.5h.

8. A method for preparing an antibacterial and deodorizing composition, characterized in that, The method for preparing the antibacterial and deodorizing composition according to any one of claims 1 to 7 is characterized by comprising the following steps: weighing plant enzymes and animal enzymes, mixing them evenly to obtain a compound biological enzyme preparation; dispersing plant extracts with an appropriate amount of the compound biological enzyme preparation, adding them to the remaining compound biological enzyme preparation, mixing them evenly, and thus obtaining the antibacterial and deodorizing composition.

9. An antibacterial and deodorizing agent, characterized in that, It comprises the antibacterial and deodorizing composition as described in any one of claims 1 to 7; The antibacterial and deodorizing agent is a liquid formulation, and the antibacterial and deodorizing agent further includes a solvent; the solvent includes a surfactant and deionized water, and the mass ratio of the antibacterial and deodorizing composition to the buffer protection system is 1:(5-10); The antibacterial and deodorizing agent is in solid form, and the antibacterial and deodorizing agent also includes a carrier; the carrier is one or more of activated carbon, zeolite, diatomaceous earth, activated clay, bentonite, and white carbon black, and the mass ratio of the antibacterial and deodorizing composition to the carrier is 1:(3-5).

10. The application of the antibacterial and deodorizing agent as described in claim 9 in pet deodorizing products.

Images